Homology
BLAST of MS020514 vs. NCBI nr
Match:
XP_022148542.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like [Momordica charantia])
HSP 1 Score: 932.2 bits (2408), Expect = 1.8e-267
Identity = 460/465 (98.92%), Postives = 462/465 (99.35%), Query Frame = 0
Query: 1 MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF
Sbjct: 1 MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
Query: 61 WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC
Sbjct: 61 WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP
Sbjct: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
GDVVILP FGAAVDEMLVL DKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMLVLKDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD
Sbjct: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQ-ERQDAMY 360
LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQ +RQDAMY
Sbjct: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQVKRQDAMY 360
Query: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELA 420
QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLI+GELA
Sbjct: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIYGELA 420
Query: 421 EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465
EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA
Sbjct: 421 EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465
BLAST of MS020514 vs. NCBI nr
Match:
XP_038902065.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X2 [Benincasa hispida])
HSP 1 Score: 797.7 bits (2059), Expect = 5.2e-227
Identity = 389/463 (84.02%), Postives = 421/463 (90.93%), Query Frame = 0
Query: 1 MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
MAISLQIPR P R F+S S G LR RKP LRCTADS SSP P+DPDFDAKLF
Sbjct: 1 MAISLQIPRFPLRIDFVSRKTFS--GVRALRCRKPLFLRCTADSSSSPEPIDPDFDAKLF 60
Query: 61 WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
WKN++RGENYNRKGFGHKEET+KLM+SEFTSE++NTLNENG +FTWGNVTVKLA AYGFC
Sbjct: 61 WKNFSRGENYNRKGFGHKEETVKLMSSEFTSEIVNTLNENGKQFTWGNVTVKLAGAYGFC 120
Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
+GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNKRL EMGV+ IPLEE KKQF+VVNP
Sbjct: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKQFNVVNP 180
Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
GDVVILP FGAAVDEM+VLNDK V+IVDTTCPWVSKVWN VDKHKK EYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMIVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHE 240
Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
ETIATASFAGKYIIVK+MAEA V D+ILGVE NG I TK EFLKKF+SAVS+GFDPD D
Sbjct: 241 ETIATASFAGKYIIVKDMAEAMDVCDYILGVEHNGLISTKAEFLKKFKSAVSKGFDPDID 300
Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQ 360
L+K+GI+NQTTMLK ETEEIGKL+E+TMMRKYGVEN+NEHF+SFNTIC+ATQ RQDAMY+
Sbjct: 301 LMKIGISNQTTMLKRETEEIGKLMEKTMMRKYGVENINEHFISFNTICNATQVRQDAMYE 360
Query: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAE 420
LVEED+DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL HGELA
Sbjct: 361 LVEEDVDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLTHGELAN 420
Query: 421 KENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
KENWLPDGH+TIGVTSGASTPDKVV NVLKKVFD+KG+GT QL
Sbjct: 421 KENWLPDGHITIGVTSGASTPDKVVGNVLKKVFDIKGDGTAQL 461
BLAST of MS020514 vs. NCBI nr
Match:
XP_004145400.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativus] >KAE8651867.1 hypothetical protein Csa_006064 [Cucumis sativus])
HSP 1 Score: 795.4 bits (2053), Expect = 2.6e-226
Identity = 386/463 (83.37%), Postives = 419/463 (90.50%), Query Frame = 0
Query: 1 MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
MAISLQ P PHR F SP S GA LR RKP LRCTADS SSP P+DPDFD KLF
Sbjct: 1 MAISLQSPCFPHRIQFFSPKTFSGVGA--LRCRKPLLLRCTADSSSSPKPVDPDFDPKLF 60
Query: 61 WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
KN++RGENYNRKGFGHKEET+KLMNSEFTSE++NTLNENG +FTWG VTVKLA AYGFC
Sbjct: 61 LKNFSRGENYNRKGFGHKEETIKLMNSEFTSEIVNTLNENGYQFTWGKVTVKLAGAYGFC 120
Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
+GVERAVQ A+EARKQ+PS TIWITNEIIHNPTVNKRL EMGVH IPLEE K+FDVVNP
Sbjct: 121 WGVERAVQFAYEARKQYPSETIWITNEIIHNPTVNKRLEEMGVHNIPLEEGNKRFDVVNP 180
Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
GDVVILP FGAAVDE+LVLNDK V+IVDTTCPWVSKVWN V KHKK EYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEILVLNDKKVKIVDTTCPWVSKVWNAVGKHKKGEYTSIIHGKYRHE 240
Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
ETIATASFAG+YIIVK+MAEA VSD+ILGVE NGSI TKEEFLKKF+SAVS+GFDPD D
Sbjct: 241 ETIATASFAGRYIIVKDMAEAACVSDYILGVEHNGSISTKEEFLKKFKSAVSKGFDPDID 300
Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQ 360
L+K+GIANQTTMLKGETEEIGKL+E+TMMRKYGVEN+NEHF+SFNTIC+ATQ RQDAMY+
Sbjct: 301 LMKIGIANQTTMLKGETEEIGKLMEKTMMRKYGVENINEHFISFNTICNATQVRQDAMYE 360
Query: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAE 420
LV+ED+DLMLVVGGWNSSNTSHLQEIAEARG+PSYWIDSEQRIGPGNSISYKL HGELA
Sbjct: 361 LVKEDVDLMLVVGGWNSSNTSHLQEIAEARGVPSYWIDSEQRIGPGNSISYKLAHGELAN 420
Query: 421 KENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
KENWLPDGH+TIGVTSGASTPDKVVE+VLKKVFD+KG+G QL
Sbjct: 421 KENWLPDGHITIGVTSGASTPDKVVEDVLKKVFDIKGDGNAQL 461
BLAST of MS020514 vs. NCBI nr
Match:
XP_023512039.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 794.7 bits (2051), Expect = 4.4e-226
Identity = 390/463 (84.23%), Postives = 422/463 (91.14%), Query Frame = 0
Query: 1 MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
MAISLQIPR+P R +S S AGA LR RKP +RCTADS S ++PDFDAKLF
Sbjct: 1 MAISLQIPRLPRRVDSVSSKSFSGAGA--LRFRKPLLVRCTADSSS----VNPDFDAKLF 60
Query: 61 WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
WKN++RGENYNRKGFG+KEE +KLM+SEFTSEV+NTLNENGN FTWGNVTVKLA AYGFC
Sbjct: 61 WKNFSRGENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFC 120
Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
+GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNKRL EMGV+ IPLEE KK+FDVVNP
Sbjct: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNP 180
Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
GDVVILP FGAAVDEMLVLNDK V+IVDTTCPWVSKVWN VDKHKK EYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHE 240
Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
ET+ATASFAGKYIIVK++ EATYV D+ILGVE NG I TKE FLKKF+SAVS+GFDPD D
Sbjct: 241 ETVATASFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSD 300
Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQ 360
L+KVGIANQTTMLKGETEEIGKLIE+TMMRKYG+EN+N+HF+SFNTIC+ATQ RQDAMYQ
Sbjct: 301 LMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQ 360
Query: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAE 420
LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL HGELA+
Sbjct: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAK 420
Query: 421 KENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
KENWLPDGHVTIGVTSGASTPDKVV NVLKKVF++KGEGT QL
Sbjct: 421 KENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQL 457
BLAST of MS020514 vs. NCBI nr
Match:
XP_022944389.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 790.0 bits (2039), Expect = 1.1e-224
Identity = 389/463 (84.02%), Postives = 422/463 (91.14%), Query Frame = 0
Query: 1 MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
MAISLQIPR+ R +S SA GA LR RKP +RCTADS S ++PDFDAKLF
Sbjct: 1 MAISLQIPRLHLRVDSVSSKRFSAGGA--LRCRKPLLVRCTADSSS----VNPDFDAKLF 60
Query: 61 WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
WKN++RGENYNRKGFGHKEET+KLM+SEFTSEV+NTLNENGN FTWGNVTVKLA AYGFC
Sbjct: 61 WKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFC 120
Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
+GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNKRL EMGV+ IPLEE KK+FDVVNP
Sbjct: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNP 180
Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
GDVVILP FGAAVDEMLVL+DK V+IVDTTCPWVSKVWN VDKHKK EYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHE 240
Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
ET+ATASFAGKYIIVK+M EATYV D+ILGVE NG I TKE FLKKF+SA+S+GFDPD D
Sbjct: 241 ETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSD 300
Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQ 360
L+KVGIANQTTMLKGETEEIGKLIE+TMMRKYG+EN+N+HF+SFNTIC+ATQ RQDAMYQ
Sbjct: 301 LMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQ 360
Query: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAE 420
LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL HGELA+
Sbjct: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAK 420
Query: 421 KENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
KENWLPDGHVTIGVTSGASTPDKVV NVLKKVF++KGEGT Q+
Sbjct: 421 KENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQV 457
BLAST of MS020514 vs. ExPASy Swiss-Prot
Match:
Q94B35 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISPH PE=1 SV=1)
HSP 1 Score: 685.3 bits (1767), Expect = 4.9e-196
Identity = 336/468 (71.79%), Postives = 394/468 (84.19%), Query Frame = 0
Query: 1 MAISLQIPRVPHRA-AFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGP---LDPDFD 60
MA++LQ R+ R F+ + LS +G+ LR RK S+RC++ ++P P +D DFD
Sbjct: 1 MAVALQFSRLCVRPDTFVRENHLSGSGS--LRRRKALSVRCSSGDENAPSPSVVMDSDFD 60
Query: 61 AKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARA 120
AK+F KN R +NYNRKGFGHKEET+KLMN E+TS+++ TL NG ++WG+VTVKLA+A
Sbjct: 61 AKVFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKA 120
Query: 121 YGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFD 180
YGFC+GVERAVQIA+EARKQFP +WITNEIIHNPTVNKRL +M V IP+E+ KKQFD
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFD 180
Query: 181 VVNPGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGK 240
VV DVVILP FGA VDEM VLNDK VQIVDTTCPWV+KVWN V+KHKK EYTS+IHGK
Sbjct: 181 VVEKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGK 240
Query: 241 YRHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFD 300
Y HEETIATASFAGKYIIVKNM EA YV D+ILG + +GS TKEEF++KF+ A+S+GFD
Sbjct: 241 YNHEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFD 300
Query: 301 PDYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQD 360
PD DL+KVGIANQTTMLKGETEEIG+L+E TMMRKYGVENV+ HF+SFNTICDATQERQD
Sbjct: 301 PDNDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQD 360
Query: 361 AMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHG 420
A+Y+LVEE +DLMLVVGGWNSSNTSHLQEI+EARGIPSYWIDSE+RIGPGN I+YKL +G
Sbjct: 361 AIYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYG 420
Query: 421 ELAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465
EL EKEN+LP G +TIGVTSGASTPDKVVE+ L KVFD+K E +QLA
Sbjct: 421 ELVEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQLA 466
BLAST of MS020514 vs. ExPASy Swiss-Prot
Match:
Q6AVG6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISPH PE=2 SV=1)
HSP 1 Score: 667.2 bits (1720), Expect = 1.4e-190
Identity = 327/455 (71.87%), Postives = 379/455 (83.30%), Query Frame = 0
Query: 13 RAAFISPSPLSAAGALPLRLRKPFSLRC---TADSPSSPGPLDPDFDAKLFWKNWARGEN 72
R+A +SP AA R P S+RC A S S+ LD DFD K F N R +N
Sbjct: 9 RSALLSP----AASPSRRARRAPSSVRCDSSAASSLSASASLDADFDKKQFRHNLTRSDN 68
Query: 73 YNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFCYGVERAVQI 132
YNRKGFGHK+ET++LM+ E+TS+VI TL ENGN+ TWG VTVKLA AYGFC+GVERAVQI
Sbjct: 69 YNRKGFGHKKETLELMSQEYTSDVIKTLKENGNQHTWGPVTVKLAEAYGFCWGVERAVQI 128
Query: 133 AFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNPGDVVILPTF 192
A+EARKQFP IW+TNEIIHNPTVNKRL +MGV IP++ K FDVV GDVV+LP F
Sbjct: 129 AYEARKQFPDDRIWLTNEIIHNPTVNKRLEDMGVQNIPVDAGIKDFDVVEQGDVVVLPAF 188
Query: 193 GAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHEETIATASFA 252
GAAV+EM LN+K VQIVDTTCPWVSKVWN+V+KHKK +YTSIIHGKY HEET+ATASFA
Sbjct: 189 GAAVEEMYTLNEKKVQIVDTTCPWVSKVWNMVEKHKKGDYTSIIHGKYSHEETVATASFA 248
Query: 253 GKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYDLIKVGIANQ 312
G YIIVKN+AEA+YV D+ILG + +GS TKEEFL+KF++AVS GFDPD DL+KVGIANQ
Sbjct: 249 GTYIIVKNIAEASYVCDYILGGQLDGSSSTKEEFLEKFKNAVSPGFDPDVDLVKVGIANQ 308
Query: 313 TTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQLVEEDMDLM 372
TTMLKGETEEIGKL+E+TMMR++GVENVN+HF++FNTICDATQERQDAMYQLV+E +DL+
Sbjct: 309 TTMLKGETEEIGKLVEKTMMRRFGVENVNDHFIAFNTICDATQERQDAMYQLVKEKVDLI 368
Query: 373 LVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAEKENWLPDGH 432
LVVGGWNSSNTSHLQEI E GIPSYWIDSEQRIGPGN ISYKL HGEL EKENWLP+G
Sbjct: 369 LVVGGWNSSNTSHLQEIGELSGIPSYWIDSEQRIGPGNKISYKLNHGELVEKENWLPEGP 428
Query: 433 VTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465
+TIGVTSGASTPDKVVE+ L+KVF++K + +Q A
Sbjct: 429 ITIGVTSGASTPDKVVEDALQKVFEIKRQEVLQAA 459
BLAST of MS020514 vs. ExPASy Swiss-Prot
Match:
B1WTZ2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (strain ATCC 51142 / BH68) OX=43989 GN=ispH PE=3 SV=1)
HSP 1 Score: 519.2 bits (1336), Expect = 4.7e-146
Identity = 249/406 (61.33%), Postives = 317/406 (78.08%), Query Frame = 0
Query: 56 DAKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLAR 115
D K F ++ + NY+RKGFGH+ E M MN+E+ S +I + EN ++ G+VT+KLA
Sbjct: 2 DTKAFKRSLQQSNNYHRKGFGHETEVMGTMNTEYQSSLIQKIRENNYQWQEGDVTIKLAE 61
Query: 116 AYGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQF 175
A+GFC+GVERAV +A+E R+ FP IWITNEIIHNP+VN+RL EM V +I + K F
Sbjct: 62 AFGFCWGVERAVAMAYETRQHFPQEKIWITNEIIHNPSVNQRLLEMNVGFIEVINGSKDF 121
Query: 176 DVVNPGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHG 235
VV GDVVILP FGA+V EM +LNDK IVDTTCPWVSKVWN V+KHKK++YTSIIHG
Sbjct: 122 SVVEAGDVVILPAFGASVTEMQLLNDKGCTIVDTTCPWVSKVWNSVEKHKKKDYTSIIHG 181
Query: 236 KYRHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGF 295
KY+HEET+AT+SFAG Y++V N+ EA YV D+IL NG K+EFL+KF++A S GF
Sbjct: 182 KYKHEETVATSSFAGTYLVVLNLKEAQYVCDYIL----NGG--NKDEFLEKFKNAHSEGF 241
Query: 296 DPDYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQ 355
DPD DLI++GIANQTTMLK ETE+IGKL E TM++KYG +NEHF+SFNTICDATQERQ
Sbjct: 242 DPDKDLIRLGIANQTTMLKSETEQIGKLFETTMLKKYGPTELNEHFMSFNTICDATQERQ 301
Query: 356 DAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIH 415
DAM LVEE++D+M+V+GG+NSSNT+HLQEI+ +GI SY IDS++RI PGN I +K +
Sbjct: 302 DAMLNLVEEEVDVMVVIGGFNSSNTTHLQEISIEKGIDSYHIDSDKRILPGNKIEHKPLE 361
Query: 416 GELAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTV 462
++ KENWLPDG + +GVTSGASTPDKVVE V++++F+ K G +
Sbjct: 362 KDIETKENWLPDGKIIVGVTSGASTPDKVVEAVIERIFEEKNAGVL 401
BLAST of MS020514 vs. ExPASy Swiss-Prot
Match:
B8HWD3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) OX=395961 GN=ispH PE=3 SV=1)
HSP 1 Score: 508.8 bits (1309), Expect = 6.3e-143
Identity = 246/402 (61.19%), Postives = 312/402 (77.61%), Query Frame = 0
Query: 56 DAKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLAR 115
D K F ++ Y+RKGFGH+ E +++ E+ S +I + ENGN F G VT++LA
Sbjct: 2 DTKAFKRSLHSSNQYHRKGFGHEAEVTEMLRFEYQSSLIQQIRENGNRFQRGEVTIQLAE 61
Query: 116 AYGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEER-KKQ 175
A+GFC+GVERAV +A+E R+ FP+ IWITNEIIHNP+VN+ L EM V +IP++E+ +K
Sbjct: 62 AFGFCWGVERAVALAYETRQHFPTERIWITNEIIHNPSVNQNLREMQVEFIPVDEQGQKD 121
Query: 176 FDVVNPGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIH 235
F VV DVVILP FGA+V EM +LNDK +I+DTTCPWVSKVWN V+KHKK YTSIIH
Sbjct: 122 FSVVAGEDVVILPAFGASVQEMQLLNDKGCKIMDTTCPWVSKVWNSVEKHKKGSYTSIIH 181
Query: 236 GKYRHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRG 295
GKY+HEETIAT+S+A Y+IV ++ EA YV ++IL NG +EEFL KF A S
Sbjct: 182 GKYQHEETIATSSYAATYLIVLDLKEAEYVCNYIL----NGG--DREEFLHKFRHAYSPD 241
Query: 296 FDPDYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQER 355
FDPD DL++VGIANQTTMLKGETE+IGKL ERT+MRKYG + +N+HF+SFNTICDATQER
Sbjct: 242 FDPDRDLVRVGIANQTTMLKGETEQIGKLFERTLMRKYGPDQINQHFLSFNTICDATQER 301
Query: 356 QDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLI 415
QDAM++LVE +DLM+V+GG+NSSNT+HLQEI+ R IPSY IDS RIGPGN + +K +
Sbjct: 302 QDAMFKLVEYPLDLMVVIGGFNSSNTTHLQEISIEREIPSYHIDSADRIGPGNRVEHKPL 361
Query: 416 HGELAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVK 457
H L KENWLP+G + +G+TSGASTPDKVV +VL+K+F +K
Sbjct: 362 HRPLEIKENWLPEGPIVVGITSGASTPDKVVSDVLEKIFALK 397
BLAST of MS020514 vs. ExPASy Swiss-Prot
Match:
B2IZV5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=ispH PE=3 SV=1)
HSP 1 Score: 506.9 bits (1304), Expect = 2.4e-142
Identity = 243/401 (60.60%), Postives = 312/401 (77.81%), Query Frame = 0
Query: 56 DAKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLAR 115
D K F ++ ENYNRKGFGH+ E + SE+ S +I + + G+VT++LA+
Sbjct: 2 DTKAFKRSLQHSENYNRKGFGHQAEVATQLQSEYQSNLIQEIRDRNYILQRGDVTIRLAQ 61
Query: 116 AYGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQF 175
A+GFC+GVERAV +A+E R+ FP+ IWITNEIIHNP+VN+R+ EM V +IP+E + K F
Sbjct: 62 AFGFCWGVERAVAMAYETRQHFPTEHIWITNEIIHNPSVNQRMQEMEVEFIPIEGKNKDF 121
Query: 176 DVVNPGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHG 235
VV GDVVILP FGA+V EM +L+DK +IVDTTCPWVSKVWN V+KHKK +YTSIIHG
Sbjct: 122 SVVGTGDVVILPAFGASVQEMQILHDKGCKIVDTTCPWVSKVWNTVEKHKKIDYTSIIHG 181
Query: 236 KYRHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGF 295
KY+HEET+AT+SFAGKY+IV N+ EA YV+D+I+ NG +EEFL KF A S GF
Sbjct: 182 KYKHEETVATSSFAGKYLIVLNLQEAQYVADYII----NGG--NREEFLTKFAKACSAGF 241
Query: 296 DPDYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQ 355
DPD DL +VGIANQTTMLK ETE+IGKL ERTM++KY +N+HF SFNTICDATQERQ
Sbjct: 242 DPDRDLERVGIANQTTMLKDETEQIGKLFERTMLQKYSPIELNQHFQSFNTICDATQERQ 301
Query: 356 DAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIH 415
DAM +LVE +++LM+V+GG+NSSNT+ LQ+IA R IPSY ID+ +RI G+SI ++ ++
Sbjct: 302 DAMLELVEHNLNLMVVIGGFNSSNTTQLQQIAFERRIPSYHIDTVERIKSGDSIEHRQLN 361
Query: 416 GELAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVK 457
G+L ENWLPDG + +G+TSGASTPDKVVE+V++K+F VK
Sbjct: 362 GQLITTENWLPDGEIVVGITSGASTPDKVVEDVIEKIFAVK 396
BLAST of MS020514 vs. ExPASy TrEMBL
Match:
A0A6J1D5C2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111017170 PE=3 SV=1)
HSP 1 Score: 932.2 bits (2408), Expect = 8.6e-268
Identity = 460/465 (98.92%), Postives = 462/465 (99.35%), Query Frame = 0
Query: 1 MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF
Sbjct: 1 MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
Query: 61 WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC
Sbjct: 61 WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP
Sbjct: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
GDVVILP FGAAVDEMLVL DKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMLVLKDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD
Sbjct: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQ-ERQDAMY 360
LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQ +RQDAMY
Sbjct: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQVKRQDAMY 360
Query: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELA 420
QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLI+GELA
Sbjct: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIYGELA 420
Query: 421 EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465
EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA
Sbjct: 421 EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465
BLAST of MS020514 vs. ExPASy TrEMBL
Match:
A0A6J1FWL7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448851 PE=3 SV=1)
HSP 1 Score: 790.0 bits (2039), Expect = 5.3e-225
Identity = 389/463 (84.02%), Postives = 422/463 (91.14%), Query Frame = 0
Query: 1 MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
MAISLQIPR+ R +S SA GA LR RKP +RCTADS S ++PDFDAKLF
Sbjct: 1 MAISLQIPRLHLRVDSVSSKRFSAGGA--LRCRKPLLVRCTADSSS----VNPDFDAKLF 60
Query: 61 WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
WKN++RGENYNRKGFGHKEET+KLM+SEFTSEV+NTLNENGN FTWGNVTVKLA AYGFC
Sbjct: 61 WKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFC 120
Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
+GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNKRL EMGV+ IPLEE KK+FDVVNP
Sbjct: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNP 180
Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
GDVVILP FGAAVDEMLVL+DK V+IVDTTCPWVSKVWN VDKHKK EYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHE 240
Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
ET+ATASFAGKYIIVK+M EATYV D+ILGVE NG I TKE FLKKF+SA+S+GFDPD D
Sbjct: 241 ETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSD 300
Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQ 360
L+KVGIANQTTMLKGETEEIGKLIE+TMMRKYG+EN+N+HF+SFNTIC+ATQ RQDAMYQ
Sbjct: 301 LMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQ 360
Query: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAE 420
LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL HGELA+
Sbjct: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAK 420
Query: 421 KENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
KENWLPDGHVTIGVTSGASTPDKVV NVLKKVF++KGEGT Q+
Sbjct: 421 KENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQV 457
BLAST of MS020514 vs. ExPASy TrEMBL
Match:
A0A1S3CAB0 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498435 PE=3 SV=1)
HSP 1 Score: 783.5 bits (2022), Expect = 4.9e-223
Identity = 382/463 (82.51%), Postives = 417/463 (90.06%), Query Frame = 0
Query: 1 MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
MAISLQ PR PHR F P S GA LR R+P LRCTADS SSP P+DPDF+AKLF
Sbjct: 1 MAISLQSPRFPHRIDFSLPKTFSGVGA--LRCRQPLLLRCTADSSSSPKPVDPDFNAKLF 60
Query: 61 WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
KN++RGENYNRKGFGHKEET+KLMNSEFTSE++ TL ENG +FTWGNVTVKLA AYGFC
Sbjct: 61 LKNFSRGENYNRKGFGHKEETVKLMNSEFTSEIVTTLKENGFQFTWGNVTVKLAGAYGFC 120
Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEE-RKKQFDVVN 180
+GVERAVQ A+EARKQ+PS TIWITNEIIHNPTVNKRL EMGVH IPLEE KK+FD VN
Sbjct: 121 WGVERAVQFAYEARKQYPSETIWITNEIIHNPTVNKRLEEMGVHNIPLEEGNKKRFDAVN 180
Query: 181 PGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRH 240
PGDVVILP FGAAVDE+LVLNDK V+IVDTTCPWVSKVWN V KHKK EYTSIIHGKY H
Sbjct: 181 PGDVVILPAFGAAVDEILVLNDKKVKIVDTTCPWVSKVWNAVGKHKKGEYTSIIHGKYSH 240
Query: 241 EETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDY 300
EETIATASFAGKYIIVK+MAEA YVSD+ILGVE +GSI TKEEFLKKF+SAVS+GFDPD
Sbjct: 241 EETIATASFAGKYIIVKDMAEAAYVSDYILGVEHDGSISTKEEFLKKFKSAVSKGFDPDI 300
Query: 301 DLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMY 360
L+K+GIANQTTMLKGETEEIGKL+E+TMMRKYGVEN+NEHF+SFNTIC+ATQ RQDAMY
Sbjct: 301 HLVKIGIANQTTMLKGETEEIGKLMEKTMMRKYGVENINEHFISFNTICNATQVRQDAMY 360
Query: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELA 420
+LV+ED+DLMLVVGGWNSSNTSHLQEIAEARG+PSYWIDSEQRIGPGNSISYKL HGELA
Sbjct: 361 ELVKEDVDLMLVVGGWNSSNTSHLQEIAEARGVPSYWIDSEQRIGPGNSISYKLAHGELA 420
Query: 421 EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQ 463
KENWLPDGH+TIGVTSGASTPDKVV +VLKKVFD+KG+GT Q
Sbjct: 421 NKENWLPDGHITIGVTSGASTPDKVVGDVLKKVFDIKGDGTAQ 461
BLAST of MS020514 vs. ExPASy TrEMBL
Match:
A0A6J1JG99 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484238 PE=3 SV=1)
HSP 1 Score: 776.2 bits (2003), Expect = 7.8e-221
Identity = 382/463 (82.51%), Postives = 416/463 (89.85%), Query Frame = 0
Query: 1 MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
MAISLQIPR+P R +S S GA LR KP +RCTADS S ++PDFDAKLF
Sbjct: 1 MAISLQIPRLPLRVDSVSSKRFSGGGA--LRCWKPLLVRCTADSSS----VNPDFDAKLF 60
Query: 61 WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
WKN++RGENYNRKGF HKEET+KLM+SEFTSEV+NTLNENGN FTWGNVTVKLA AYGFC
Sbjct: 61 WKNFSRGENYNRKGFEHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFC 120
Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
+GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNKRL EMGV+ IPLEE KK+FDVVNP
Sbjct: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNP 180
Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
GDVVILP FGAAVDEMLVL+DK V IVDTTCPWVSKVWN VDKHKK EYTSIIHGKY HE
Sbjct: 181 GDVVILPAFGAAVDEMLVLSDKKVTIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYHHE 240
Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
ET+ATASFAGKYIIVK+M EATYV D+ILGVE NG I TKE+FLKKF+SAVS+GFDPD D
Sbjct: 241 ETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEDFLKKFKSAVSKGFDPDSD 300
Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQ 360
L+KVGIANQTTMLK ET+EIGKLIE+TMMRKYG+EN++EHF+SFNTIC+ATQ RQDAMYQ
Sbjct: 301 LMKVGIANQTTMLKEETKEIGKLIEKTMMRKYGIENISEHFISFNTICNATQVRQDAMYQ 360
Query: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAE 420
LVEED DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL HGEL +
Sbjct: 361 LVEEDTDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLKHGELVK 420
Query: 421 KENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
KENWLPDGH+TIGVTSGASTPD+VV NVLKKVF++KGEGT QL
Sbjct: 421 KENWLPDGHITIGVTSGASTPDQVVGNVLKKVFEIKGEGTAQL 457
BLAST of MS020514 vs. ExPASy TrEMBL
Match:
A0A2N9ECS7 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS380 PE=3 SV=1)
HSP 1 Score: 735.3 bits (1897), Expect = 1.5e-208
Identity = 361/466 (77.47%), Postives = 402/466 (86.27%), Query Frame = 0
Query: 1 MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPG---PLDPDFDA 60
MAISLQ+ R+P R P L+ GA LRLRKP S+RCT DS SS +D DFDA
Sbjct: 1 MAISLQLCRIPTRTDLFLPESLAGPGA--LRLRKPLSVRCTGDSSSSSSSSVAVDSDFDA 60
Query: 61 KLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAY 120
K+F KN R +NYNRKGFGHKEET++LMN E+TS++I TL ENGNE+TWGNVTVKLA AY
Sbjct: 61 KVFRKNLTRSKNYNRKGFGHKEETLELMNREYTSDIIKTLKENGNEYTWGNVTVKLAEAY 120
Query: 121 GFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDV 180
GFC+GVERAVQIA+EARKQFP IWITNEIIHNPTVNKRL EM V IP+EE +KQF+V
Sbjct: 121 GFCWGVERAVQIAYEARKQFPDEKIWITNEIIHNPTVNKRLEEMEVQNIPIEEGQKQFEV 180
Query: 181 VNPGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKY 240
VN GDVV+LP FGAAVDEML L+DKNVQIVDTTCPWVSKVWN V+KHKK +YTSIIHGKY
Sbjct: 181 VNKGDVVVLPAFGAAVDEMLTLSDKNVQIVDTTCPWVSKVWNTVEKHKKGDYTSIIHGKY 240
Query: 241 RHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDP 300
HEET+ATASFAGKYIIVKNM EA YV D+ILG E NGS TKEEFL+KF+ AVS+GFDP
Sbjct: 241 AHEETVATASFAGKYIIVKNMTEAMYVCDYILGGELNGSSSTKEEFLEKFKYAVSKGFDP 300
Query: 301 DYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDA 360
D DL+K+GIANQTTMLKGETEEIGKL+ERTMMRKYGVENVNEHF+SFNTICDATQERQDA
Sbjct: 301 DSDLLKLGIANQTTMLKGETEEIGKLVERTMMRKYGVENVNEHFISFNTICDATQERQDA 360
Query: 361 MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGE 420
MY+LVEE +DLMLV+GGWNSSNTSHLQEIAE RGIPSYWIDSEQR+GPGN I+YKL HGE
Sbjct: 361 MYKLVEEKVDLMLVIGGWNSSNTSHLQEIAEDRGIPSYWIDSEQRVGPGNKIAYKLNHGE 420
Query: 421 LAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
L EKENWLP+G +TIGVTSGASTPDKVVE+VL KVFD+K E +QL
Sbjct: 421 LVEKENWLPEGPITIGVTSGASTPDKVVEDVLIKVFDIKREEALQL 464
BLAST of MS020514 vs. TAIR 10
Match:
AT4G34350.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase )
HSP 1 Score: 685.3 bits (1767), Expect = 3.5e-197
Identity = 336/468 (71.79%), Postives = 394/468 (84.19%), Query Frame = 0
Query: 1 MAISLQIPRVPHRA-AFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGP---LDPDFD 60
MA++LQ R+ R F+ + LS +G+ LR RK S+RC++ ++P P +D DFD
Sbjct: 1 MAVALQFSRLCVRPDTFVRENHLSGSGS--LRRRKALSVRCSSGDENAPSPSVVMDSDFD 60
Query: 61 AKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARA 120
AK+F KN R +NYNRKGFGHKEET+KLMN E+TS+++ TL NG ++WG+VTVKLA+A
Sbjct: 61 AKVFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKA 120
Query: 121 YGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFD 180
YGFC+GVERAVQIA+EARKQFP +WITNEIIHNPTVNKRL +M V IP+E+ KKQFD
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFD 180
Query: 181 VVNPGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGK 240
VV DVVILP FGA VDEM VLNDK VQIVDTTCPWV+KVWN V+KHKK EYTS+IHGK
Sbjct: 181 VVEKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGK 240
Query: 241 YRHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFD 300
Y HEETIATASFAGKYIIVKNM EA YV D+ILG + +GS TKEEF++KF+ A+S+GFD
Sbjct: 241 YNHEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFD 300
Query: 301 PDYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQD 360
PD DL+KVGIANQTTMLKGETEEIG+L+E TMMRKYGVENV+ HF+SFNTICDATQERQD
Sbjct: 301 PDNDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQD 360
Query: 361 AMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHG 420
A+Y+LVEE +DLMLVVGGWNSSNTSHLQEI+EARGIPSYWIDSE+RIGPGN I+YKL +G
Sbjct: 361 AIYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYG 420
Query: 421 ELAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465
EL EKEN+LP G +TIGVTSGASTPDKVVE+ L KVFD+K E +QLA
Sbjct: 421 ELVEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQLA 466
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022148542.1 | 1.8e-267 | 98.92 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like [Momordic... | [more] |
| XP_038902065.1 | 5.2e-227 | 84.02 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... | [more] |
| XP_004145400.1 | 2.6e-226 | 83.37 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativ... | [more] |
| XP_023512039.1 | 4.4e-226 | 84.23 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... | [more] |
| XP_022944389.1 | 1.1e-224 | 84.02 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q94B35 | 4.9e-196 | 71.79 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis... | [more] |
| Q6AVG6 | 1.4e-190 | 71.87 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativ... | [more] |
| B1WTZ2 | 4.7e-146 | 61.33 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (s... | [more] |
| B8HWD3 | 6.3e-143 | 61.19 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC... | [more] |
| B2IZV5 | 2.4e-142 | 60.60 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Nostoc punctiforme (strain... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1D5C2 | 8.6e-268 | 98.92 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momord... | [more] |
| A0A6J1FWL7 | 5.3e-225 | 84.02 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... | [more] |
| A0A1S3CAB0 | 4.9e-223 | 82.51 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... | [more] |
| A0A6J1JG99 | 7.8e-221 | 82.51 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... | [more] |
| A0A2N9ECS7 | 1.5e-208 | 77.47 | Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS380 PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G34350.1 | 3.5e-197 | 71.79 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase | [more] |
