MS017950.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS017950.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Phytocyanin domain-containing protein
Location	scaffold373: 4084980 .. 4086577 (+)
Sequence length	546
RNA-Seq Expression	MS017950.1
Synteny	MS017950.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAAATAAGAAGGTGTGGCTTCATGTGTTTTTTATGATAATTGCAGGTTTTGCATTAAGATCATGCACTGCCACAACATACATTGTGGGAGATTCTTCTGGTTGGGATATCAGCACTGATCTTGATACTTGGTCTCAGGGCAAGAGATTTTTTGTGGGAGATCTTCTTGGTACGTAATTCTCTTTCTTCTTGATCGAGTCCTTATTACTACTAAATGTTTGAAGAATGGTGTGTTATGTGAAATTTAAGAATGTTCATTTTGATATGTTGTTAGTAGAGTCGAATAATAAGTTATTATGGGCCAATGTTATATCTATGTATTAGGATCAAATTAAACATAGACTATATTCTCAATTATAAGTATATAATTTGATGTTGCATCCTAGTTTTATCAATAAATTTAAGATTTTGTGAGAGTGAAGACTTGGATTGAAAAATCAATTGAAAAGGCTCCACATAATTAACTAAGTTAAGTATTTAAGGTTAAATTTAAATTTTAGTTCATAAGCTTAAATAATTTGTCTCTTTATCCCTATACGTTTCATTTTAATTTTCACAAACATCATTAATAAATGTTAAGTGACGAGTGAATAATGATTTGATATGACATGACACAAGTAAATAGATTAATGAGATATATAGAATAAAGATAGATGAGAGAAAAAAGAAAAGATCTAGAACGTACTCCACCTAATCTTATCCAATTAGTTACTGTTGAAGTGTGAGGTGCAAGAGTACTGAACATGACTCTTGTAGAAGAGAAAGAAATGCGGTAAAGAGAAGTTTCAAGCATAGGAGTTCATTCCATGTTTTTCACCCCACATTCCTTCTTAGTTTGTATAGTTAGTCGGTGCTAACTACCAAATTATCATTTGTTAGTTCAATCAACATGTCAGGGCAACATTTTTCTTAATATACGTGAGTATTCGAGCTAGTTTATGCACACCTCAACTAATTTTAGAGGACAGCCGTCTAGTCCTCTAACGTTTGATCATGAGATTTGAAACTCATTCACTCTTAATTTATCCTTAGCATAGCTACCTAGCCTGCCTCCTTGGACCAATTAGCTACGGTGATGTTAATATTTAACTAACCAGATTGACAAGACATGATTGAAAAAGTTTCTTAAAATTTAAGAATTTGAGTAGAAAAACAACATAAAAATGGTGAGACGAGAGAGATTTTGAAATAATTATTGGCGAAATGTAATTTGTCTTGCAGTTTTCCAATACTCATCATCGGAGAGCTTAAACGAAGTGACGAGAGAGAATTTCGACAGCTGCAACACCGCCGCCGTGCTGAAAGCCTACTCCACCGGAAACACCACCGTGACGCTGTCGGAGCCGGGGCAGAGGTTCTTCGTCTCCGGCAACAGGCTGTACTGTCTCGGCGGCATGAAGCTACAAGTCAACGTCGAGAACAACCAATCATTCTCGCCGGCCGCCGCTCCTCAGCCCAACGGCACCGCCCTCCCCCGCCCTTCTTCCAAAACCGATGACACCGTTCCCGGCGCTTCGGCCGCCTCTATAATCGGTGGAAGAGAGGCTCTTGTGCTTGCATTTGTGTGTTACGTGATCTCTACTCTCATGGTGGTCTTT

mRNA sequence

CCAAATAAGAAGGTGTGGCTTCATGTGTTTTTTATGATAATTGCAGGTTTTGCATTAAGATCATGCACTGCCACAACATACATTGTGGGAGATTCTTCTGGTTGGGATATCAGCACTGATCTTGATACTTGGTCTCAGGGCAAGAGATTTTTTGTGGGAGATCTTCTTGTTTTCCAATACTCATCATCGGAGAGCTTAAACGAAGTGACGAGAGAGAATTTCGACAGCTGCAACACCGCCGCCGTGCTGAAAGCCTACTCCACCGGAAACACCACCGTGACGCTGTCGGAGCCGGGGCAGAGGTTCTTCGTCTCCGGCAACAGGCTGTACTGTCTCGGCGGCATGAAGCTACAAGTCAACGTCGAGAACAACCAATCATTCTCGCCGGCCGCCGCTCCTCAGCCCAACGGCACCGCCCTCCCCCGCCCTTCTTCCAAAACCGATGACACCGTTCCCGGCGCTTCGGCCGCCTCTATAATCGGTGGAAGAGAGGCTCTTGTGCTTGCATTTGTGTGTTACGTGATCTCTACTCTCATGGTGGTCTTT

Coding sequence (CDS)

CCAAATAAGAAGGTGTGGCTTCATGTGTTTTTTATGATAATTGCAGGTTTTGCATTAAGATCATGCACTGCCACAACATACATTGTGGGAGATTCTTCTGGTTGGGATATCAGCACTGATCTTGATACTTGGTCTCAGGGCAAGAGATTTTTTGTGGGAGATCTTCTTGTTTTCCAATACTCATCATCGGAGAGCTTAAACGAAGTGACGAGAGAGAATTTCGACAGCTGCAACACCGCCGCCGTGCTGAAAGCCTACTCCACCGGAAACACCACCGTGACGCTGTCGGAGCCGGGGCAGAGGTTCTTCGTCTCCGGCAACAGGCTGTACTGTCTCGGCGGCATGAAGCTACAAGTCAACGTCGAGAACAACCAATCATTCTCGCCGGCCGCCGCTCCTCAGCCCAACGGCACCGCCCTCCCCCGCCCTTCTTCCAAAACCGATGACACCGTTCCCGGCGCTTCGGCCGCCTCTATAATCGGTGGAAGAGAGGCTCTTGTGCTTGCATTTGTGTGTTACGTGATCTCTACTCTCATGGTGGTCTTT

Protein sequence

PNKKVWLHVFFMIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYSSSESLNEVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNVENNQSFSPAAAPQPNGTALPRPSSKTDDTVPGASAASIIGGREALVLAFVCYVISTLMVVF

Homology

[Close]

BLAST of MS017950.1 vs. NCBI nr
Match: XP_038877141.1 (stellacyanin-like [Benincasa hispida])

HSP 1 Score: 268.9 bits (686), Expect = 3.3e-68
Identity = 139/184 (75.54%), Postives = 156/184 (84.78%), Query Frame = 0


Query: 2   NKKVWLHVFFMIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYS 61
           N  +W+ V  ++I GFALR C ATTYIVGD+SGWDISTDLDTWSQGKRFFVGD+LVFQYS
Sbjct: 3   NNNIWVMV-LVVIGGFALR-CNATTYIVGDTSGWDISTDLDTWSQGKRFFVGDVLVFQYS 62

Query: 62  SSESLNEVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNV 121
           SS SLNEVTRENF++CNT  VLKAYS+GNTT+TLS+PGQRFFVS NRL CLGGMKLQVNV
Sbjct: 63  SSASLNEVTRENFNTCNTTNVLKAYSSGNTTITLSQPGQRFFVSANRLLCLGGMKLQVNV 122

Query: 122 ENNQSFSPAAAPQP--NGTALPRPSSKTDDT-VPGASAASIIGGREALVLAFVCYVISTL 181
           ENNQSFSPA APQP      LPRPSSKTD+  VP ASA+ +IGG+EAL + FVCYVIST 
Sbjct: 123 ENNQSFSPATAPQPPTQSDTLPRPSSKTDNNGVPSASASFVIGGKEALAIVFVCYVISTS 182

Query: 182 MVVF 183
            +VF
Sbjct: 183 FIVF 184

[Close]

BLAST of MS017950.1 vs. NCBI nr
Match: XP_011654854.1 (blue copper protein [Cucumis sativus] >KGN50322.1 hypothetical protein Csa_000514 [Cucumis sativus])

HSP 1 Score: 263.1 bits (671), Expect = 1.8e-66
Identity = 137/184 (74.46%), Postives = 151/184 (82.07%), Query Frame = 0


Query: 2   NKKVWLHVFFMIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYS 61
           N  +W+ V  ++I GFA   C ATTYIVGD+SGWDISTDLDTWSQGKRFFVGD+LVFQYS
Sbjct: 5   NNNIWVCV-LVVIFGFAFIRCNATTYIVGDTSGWDISTDLDTWSQGKRFFVGDVLVFQYS 64

Query: 62  SSESLNEVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNV 121
           S  SLNEVTRENF+SCNT  VLKAYS+GNTTVTLSEPG RFFVSGNRL CLGGMKLQVNV
Sbjct: 65  SLASLNEVTRENFNSCNTTNVLKAYSSGNTTVTLSEPGHRFFVSGNRLLCLGGMKLQVNV 124

Query: 122 ENNQSFSPAAAPQP---NGTALPRPSSKTD-DTVPGASAASIIGGREALVLAFVCYVIST 181
           ENNQSFSPAAAPQP      ALPRPSSKTD ++VP A+A  +IGG + L   FVCYVIST
Sbjct: 125 ENNQSFSPAAAPQPPSSQSDALPRPSSKTDNNSVPSAAAGVVIGGNQGLAFLFVCYVIST 184

[Close]

BLAST of MS017950.1 vs. NCBI nr
Match: XP_023549499.1 (stellacyanin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 255.8 bits (652), Expect = 2.9e-64
Identity = 132/181 (72.93%), Postives = 150/181 (82.87%), Query Frame = 0


Query: 3   KKVWLHVFFMIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYSS 62
           K +W++V  +II G AL SC ATTYIVGD+SGWDISTDLD WSQGKRF+VGD+LVFQYSS
Sbjct: 7   KNIWVYV-LVIIGGLAL-SCNATTYIVGDTSGWDISTDLDAWSQGKRFYVGDVLVFQYSS 66

Query: 63  SESLNEVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNVE 122
           S+SLNEVTREN+++CNT  VLKAY+ GNTTVTLS+PGQRFFVSGNRL CLGGMK+QVNVE
Sbjct: 67  SQSLNEVTRENYNTCNTTNVLKAYTNGNTTVTLSQPGQRFFVSGNRLLCLGGMKMQVNVE 126

Query: 123 NNQSFSPAAAPQ--PNGTALPRPSSKTD-DTVPGASAASIIGGREALVLAFVCYVISTLM 181
           NNQSFSPAAAPQ  P    LPRPSSKTD D VP  S   +IGG+EAL + F CYVIS   
Sbjct: 127 NNQSFSPAAAPQPPPQSNGLPRPSSKTDNDGVPSGSTRLVIGGKEALGIVFACYVISLAF 185

[Close]

BLAST of MS017950.1 vs. NCBI nr
Match: XP_008436972.1 (PREDICTED: stellacyanin [Cucumis melo])

HSP 1 Score: 253.4 bits (646), Expect = 1.4e-63
Identity = 132/182 (72.53%), Postives = 150/182 (82.42%), Query Frame = 0


Query: 2   NKKVWLHVFFMIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYS 61
           N   W+ V  ++I GFA   C ATTYIVGD+SGWDISTDLDTWSQGKRFFVGD+LVFQYS
Sbjct: 6   NNNTWVCV-LVVIFGFAFIRCNATTYIVGDTSGWDISTDLDTWSQGKRFFVGDVLVFQYS 65

Query: 62  SSESLNEVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNV 121
           SS SLNEVTRENF++CNT  VLKAYS+GNTTVTLS+PGQRFFVSGNRL CLGGMKLQVNV
Sbjct: 66  SSASLNEVTRENFNTCNTTNVLKAYSSGNTTVTLSKPGQRFFVSGNRLLCLGGMKLQVNV 125

Query: 122 ENNQSFSPAAAPQP----NGTALPRPSSKTD-DTVPGASAASIIGGREALVLAFVCYVIS 179
           ENNQSFSPAAAPQP    +   LPRPSSKTD ++VP  +A  ++ G++A  + FVC VIS
Sbjct: 126 ENNQSFSPAAAPQPPPSSHSDDLPRPSSKTDNNSVPNTAAGFVMEGKQARAILFVCCVIS 185

[Close]

BLAST of MS017950.1 vs. NCBI nr
Match: XP_022969745.1 (stellacyanin [Cucurbita maxima])

HSP 1 Score: 251.9 bits (642), Expect = 4.2e-63
Identity = 129/180 (71.67%), Postives = 149/180 (82.78%), Query Frame = 0


Query: 5   VWLHVFFMIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYSSSE 64
           +W++V  +II G AL SC A TYIVGD+SGWDISTDLDTWSQGKRF+VGD+LVFQYSSS+
Sbjct: 7   IWVYV-LVIIGGLAL-SCNAATYIVGDTSGWDISTDLDTWSQGKRFYVGDVLVFQYSSSQ 66

Query: 65  SLNEVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNVENN 124
           SLNEVTREN+++CNT  VLKAY+ GNTTVTLS+PGQRFFVSGNRL CLGGMK+QVNVENN
Sbjct: 67  SLNEVTRENYNTCNTTKVLKAYTNGNTTVTLSQPGQRFFVSGNRLLCLGGMKMQVNVENN 126

Query: 125 QSFSPAAAPQ--PNGTALPRPSSKTD-DTVPGASAASIIGGREALVLAFVCYVISTLMVV 182
           QSFSPAAAPQ  P    LPRPSSKTD D +P  S   +IGG+EAL +   CYVIS   ++
Sbjct: 127 QSFSPAAAPQPPPQSDGLPRPSSKTDNDGIPSGSTRHVIGGKEALGIVLACYVISLAFII 184

[Close]

BLAST of MS017950.1 vs. ExPASy Swiss-Prot
Match: O82081 (Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.5e-18
Identity = 53/141 (37.59%), Postives = 77/141 (54.61%), Query Frame = 0


Query: 9   VFFMIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYSSS-ESLN 68
           +   ++A   +    AT + +G  SGW +   L TW+ G+ F VGD LVF Y ++   + 
Sbjct: 8   IIISVLATTLIGLTVATDHTIGGPSGWTVGASLRTWAAGQTFAVGDNLVFSYPAAFHDVV 67

Query: 69  EVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNVENNQSF 128
           EVT+  FDSC     L  ++ GN+ V L+ PG+R+F+ G   +C  GMKL+VNV    + 
Sbjct: 68  EVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNVVPTATV 127

Query: 129 SPAAAPQPN---GTALPRPSS 146
           +P  AP PN       P PSS
Sbjct: 128 AP-TAPLPNTVPSLNAPSPSS 147

[Close]

BLAST of MS017950.1 vs. ExPASy Swiss-Prot
Match: Q02917 (Early nodulin-55-2 OS=Glycine max OX=3847 GN=ENOD55-2 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-13
Identity = 57/176 (32.39%), Postives = 87/176 (49.43%), Query Frame = 0


Query: 1   PNKKVWLHVFFMIIAGFALRSCTATTYIVGDS-SGWDI----STDLDTWSQGKRFFVGDL 60
           PN   +L +  M +    + +  A  Y+VG S   W         L  W+   RF +GD 
Sbjct: 6   PNASPFLVMLAMCL---LISTSEAEKYVVGGSEKSWKFPLSKPDSLSHWANSHRFKIGDT 65

Query: 61  LVFQYSS-SESLNEVTRENFDSCNTAAVLK-AYSTGNTTVTLSEPGQRFFVSGNRLYCLG 120
           L+F+Y   +ES++E    +++ CNT       ++ GNT V L++PG R F+SGN+ +C  
Sbjct: 66  LIFKYEKRTESVHEGNETDYEGCNTVGKYHIVFNGGNTKVMLTKPGFRHFISGNQSHCQM 125

Query: 121 GMKLQVNV-------ENNQSFSPAAAPQPNGTALPRPSS-KTDDTVPGASAASIIG 162
           G+KL V V       +N  S SP+ +P P+    P PS    +  V  +S A  IG
Sbjct: 126 GLKLAVLVISSNKTKKNLLSPSPSPSPPPSSLLSPSPSPLPNNQGVTSSSGAGFIG 178

[Close]

BLAST of MS017950.1 vs. ExPASy Swiss-Prot
Match: Q9SK27 (Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 1.2e-12
Identity = 57/150 (38.00%), Postives = 78/150 (52.00%), Query Frame = 0


Query: 7   LHV-FFMIIAGFALRSCTATTYIVGDSSGWDI----STDLDTWSQGKRFFVGDLLVFQY- 66
           LHV  F +I  F+L +    T + G S  W I    S     W+Q  RF VGD +VF+Y 
Sbjct: 12  LHVAIFSLIFLFSLAAANEVT-VGGKSGDWKIPPSSSYSFTEWAQKARFKVGDFIVFRYE 71

Query: 67  SSSESLNEVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVN 126
           S  +S+ EVT+E ++SCNT   L  Y+ G T V L   G  +F+SG   +C  G KL + 
Sbjct: 72  SGKDSVLEVTKEAYNSCNTTNPLANYTDGETKVKLDRSGPFYFISGANGHCEKGQKLSLV 131

Query: 127 VENNQSFSPAAAPQP----NGTAL-PRPSS 146
           V + +    + AP P    +G AL P P S
Sbjct: 132 VISPRHSVISPAPSPVEFEDGPALAPAPIS 160

[Close]

BLAST of MS017950.1 vs. ExPASy Swiss-Prot
Match: Q8LC95 (Early nodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g25090 PE=2 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 1.2e-12
Identity = 57/176 (32.39%), Postives = 86/176 (48.86%), Query Frame = 0


Query: 10  FFMIIAGFALRSCTATTYIVGDSSGWDI----STDLDTWSQGKRFFVGDLLVFQY-SSSE 69
           FF+I        C+    + G +S W I    S  L+ W++  RF VGD LV++Y    +
Sbjct: 10  FFLIFFLLTNLVCSKEIIVGGKTSSWKIPSSPSESLNKWAESLRFRVGDTLVWKYDEEKD 69

Query: 70  SLNEVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNVENN 129
           S+ +VT++ + +CNT      YS G+T V L   G  FF+SG++  C+ G KL + V ++
Sbjct: 70  SVLQVTKDAYINCNTTNPAANYSNGDTKVKLERSGPYFFISGSKSNCVEGEKLHIVVMSS 129

Query: 130 Q---------SFSPAAAPQPNGTALPRPSSKTDDTVPGASAASIIGGREALVLAFV 172
           +           SP+ AP P     P     T     G SA+S+   R+  VL FV
Sbjct: 130 RGGHTGGFFTGSSPSPAPSPALLGAP-----TVAPASGGSASSLT--RQVGVLGFV 178

[Close]

BLAST of MS017950.1 vs. ExPASy Swiss-Prot
Match: A0A0M4FTF3 (Blue copper protein OS=Petunia hybrida OX=4102 GN=BCP PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.3e-11
Identity = 39/116 (33.62%), Postives = 63/116 (54.31%), Query Frame = 0


Query: 25  TTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYSSS-ESLNEVTRENFDSCNTAAVL 84
           T Y VGD  GW I  D   W++GK F VGD LVF+Y+    ++ +V +  F +C      
Sbjct: 116 TEYWVGDDKGWTIDVDYQAWAKGKTFKVGDTLVFKYTKGHHNVFKVNQTGFQNCIAPPPS 175

Query: 85  KAYSTGNTTVTLSEPGQRFFVSGNRLYCL-GGMKLQVNVENNQSFSP---AAAPQP 136
           +  ++G+  +TL+ PG+++++ G   +C     KL + VE   + +P   A AP P
Sbjct: 176 EGLTSGHDVITLAAPGKKWYICGFPTHCSEHKQKLAITVEGAPAQTPPVWAPAPAP 231

[Close]

BLAST of MS017950.1 vs. ExPASy TrEMBL
Match: A0A0A0KPG5 (Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G167140 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 8.8e-67
Identity = 137/184 (74.46%), Postives = 151/184 (82.07%), Query Frame = 0


Query: 2   NKKVWLHVFFMIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYS 61
           N  +W+ V  ++I GFA   C ATTYIVGD+SGWDISTDLDTWSQGKRFFVGD+LVFQYS
Sbjct: 5   NNNIWVCV-LVVIFGFAFIRCNATTYIVGDTSGWDISTDLDTWSQGKRFFVGDVLVFQYS 64

Query: 62  SSESLNEVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNV 121
           S  SLNEVTRENF+SCNT  VLKAYS+GNTTVTLSEPG RFFVSGNRL CLGGMKLQVNV
Sbjct: 65  SLASLNEVTRENFNSCNTTNVLKAYSSGNTTVTLSEPGHRFFVSGNRLLCLGGMKLQVNV 124

Query: 122 ENNQSFSPAAAPQP---NGTALPRPSSKTD-DTVPGASAASIIGGREALVLAFVCYVIST 181
           ENNQSFSPAAAPQP      ALPRPSSKTD ++VP A+A  +IGG + L   FVCYVIST
Sbjct: 125 ENNQSFSPAAAPQPPSSQSDALPRPSSKTDNNSVPSAAAGVVIGGNQGLAFLFVCYVIST 184

[Close]

BLAST of MS017950.1 vs. ExPASy TrEMBL
Match: A0A1S3AT20 (stellacyanin OS=Cucumis melo OX=3656 GN=LOC103482542 PE=4 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 6.9e-64
Identity = 132/182 (72.53%), Postives = 150/182 (82.42%), Query Frame = 0


Query: 2   NKKVWLHVFFMIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYS 61
           N   W+ V  ++I GFA   C ATTYIVGD+SGWDISTDLDTWSQGKRFFVGD+LVFQYS
Sbjct: 6   NNNTWVCV-LVVIFGFAFIRCNATTYIVGDTSGWDISTDLDTWSQGKRFFVGDVLVFQYS 65

Query: 62  SSESLNEVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNV 121
           SS SLNEVTRENF++CNT  VLKAYS+GNTTVTLS+PGQRFFVSGNRL CLGGMKLQVNV
Sbjct: 66  SSASLNEVTRENFNTCNTTNVLKAYSSGNTTVTLSKPGQRFFVSGNRLLCLGGMKLQVNV 125

Query: 122 ENNQSFSPAAAPQP----NGTALPRPSSKTD-DTVPGASAASIIGGREALVLAFVCYVIS 179
           ENNQSFSPAAAPQP    +   LPRPSSKTD ++VP  +A  ++ G++A  + FVC VIS
Sbjct: 126 ENNQSFSPAAAPQPPPSSHSDDLPRPSSKTDNNSVPNTAAGFVMEGKQARAILFVCCVIS 185

[Close]

BLAST of MS017950.1 vs. ExPASy TrEMBL
Match: A0A6J1I0T8 (stellacyanin OS=Cucurbita maxima OX=3661 GN=LOC111468850 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 2.0e-63
Identity = 129/180 (71.67%), Postives = 149/180 (82.78%), Query Frame = 0


Query: 5   VWLHVFFMIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYSSSE 64
           +W++V  +II G AL SC A TYIVGD+SGWDISTDLDTWSQGKRF+VGD+LVFQYSSS+
Sbjct: 7   IWVYV-LVIIGGLAL-SCNAATYIVGDTSGWDISTDLDTWSQGKRFYVGDVLVFQYSSSQ 66

Query: 65  SLNEVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNVENN 124
           SLNEVTREN+++CNT  VLKAY+ GNTTVTLS+PGQRFFVSGNRL CLGGMK+QVNVENN
Sbjct: 67  SLNEVTRENYNTCNTTKVLKAYTNGNTTVTLSQPGQRFFVSGNRLLCLGGMKMQVNVENN 126

Query: 125 QSFSPAAAPQ--PNGTALPRPSSKTD-DTVPGASAASIIGGREALVLAFVCYVISTLMVV 182
           QSFSPAAAPQ  P    LPRPSSKTD D +P  S   +IGG+EAL +   CYVIS   ++
Sbjct: 127 QSFSPAAAPQPPPQSDGLPRPSSKTDNDGIPSGSTRHVIGGKEALGIVLACYVISLAFII 184

[Close]

BLAST of MS017950.1 vs. ExPASy TrEMBL
Match: A0A6J1ERB5 (stellacyanin OS=Cucurbita moschata OX=3662 GN=LOC111435827 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 7.7e-63
Identity = 129/172 (75.00%), Postives = 144/172 (83.72%), Query Frame = 0


Query: 12  MIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYSSSESLNEVTR 71
           MII G AL SC A TYIVGD+SGWDISTDLD+WSQGKRF+VGD+LVFQYSSS+SLNEVTR
Sbjct: 13  MIIGGLAL-SCNAATYIVGDTSGWDISTDLDSWSQGKRFYVGDVLVFQYSSSQSLNEVTR 72

Query: 72  ENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNVENNQSFSPAA 131
           EN+++CNT  VLKAY+ GNTTVTLS+PGQRFFVSGNRL CLGGMK+QVNVENNQSFSPAA
Sbjct: 73  ENYNTCNTTNVLKAYTNGNTTVTLSQPGQRFFVSGNRLLCLGGMKMQVNVENNQSFSPAA 132

Query: 132 APQ--PNGTALPRPSSKTD-DTVPGASAASIIGGREALVLAFVCYVISTLMV 181
           APQ  P    LPRPSSKTD D VP  S   +IGG+EAL + F CYVIS   +
Sbjct: 133 APQPPPQSDGLPRPSSKTDNDGVPSGSTRLVIGGKEALGIVFACYVISLAFI 183

[Close]

BLAST of MS017950.1 vs. ExPASy TrEMBL
Match: A0A5A7UGX7 (Stellacyanin OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00080 PE=4 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 5.0e-62
Identity = 129/178 (72.47%), Postives = 148/178 (83.15%), Query Frame = 0


Query: 6   WLHVFFMIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYSSSES 65
           W+ V  ++I GFA   C ATTYIVGD+SGWDISTDLDTWSQGKRFFVGD+LVFQYSSS S
Sbjct: 10  WVCV-LVVIFGFAFIRCNATTYIVGDTSGWDISTDLDTWSQGKRFFVGDVLVFQYSSSAS 69

Query: 66  LNEVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNVENNQ 125
           LNEVTRENF++CNT  VLKAYS+GNTTVTLS+PGQRFF SGNRL CLGGMKLQVNVENNQ
Sbjct: 70  LNEVTRENFNTCNTTNVLKAYSSGNTTVTLSKPGQRFFASGNRLLCLGGMKLQVNVENNQ 129

Query: 126 SFSPAAAPQP----NGTALPRPSSKTD-DTVPGASAASIIGGREALVLAFVCYVISTL 179
           SFSPAAAPQP    +   LP+PSSKTD ++VP  +A  ++ G++A  + FVC VISTL
Sbjct: 130 SFSPAAAPQPPPSSHSDDLPQPSSKTDNNSVPNTAAGFVMEGKQARAILFVCCVISTL 186

[Close]

BLAST of MS017950.1 vs. TAIR 10
Match: AT5G07475.1 (Cupredoxin superfamily protein )

HSP 1 Score: 159.1 bits (401), Expect = 3.4e-39
Identity = 78/153 (50.98%), Postives = 108/153 (70.59%), Query Frame = 0


Query: 12  MIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYSSSESLNEVTR 71
           +I     +R C ATTY VGDSSGWDIS+DL++W+ GKRF  GD+L+FQYSS+ S+ EV +
Sbjct: 15  IIFGVVVIRRCNATTYFVGDSSGWDISSDLESWTSGKRFSPGDVLMFQYSSTHSVYEVAK 74

Query: 72  ENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNVE-NNQSFSPA 131
           +N+ +CNT   ++ ++ GNTTV LS+PG RFFV GNRL+C  GM+L VNVE N  S +P 
Sbjct: 75  DNYQNCNTTDAIRTFTNGNTTVALSKPGNRFFVCGNRLHCFAGMRLLVNVEGNGPSQAPV 134

Query: 132 AAPQPNGTALPRPSSKTDDTVPG--ASAASIIG 162
            +PQ   + + +PSSK ++   G  +SAA  +G
Sbjct: 135 GSPQAATSGILQPSSKKNNPATGVASSAARFVG 167

[Close]

BLAST of MS017950.1 vs. TAIR 10
Match: AT2G32300.1 (uclacyanin 1 )

HSP 1 Score: 93.6 bits (231), Expect = 1.8e-19
Identity = 53/141 (37.59%), Postives = 77/141 (54.61%), Query Frame = 0


Query: 9   VFFMIIAGFALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYSSS-ESLN 68
           +   ++A   +    AT + +G  SGW +   L TW+ G+ F VGD LVF Y ++   + 
Sbjct: 8   IIISVLATTLIGLTVATDHTIGGPSGWTVGASLRTWAAGQTFAVGDNLVFSYPAAFHDVV 67

Query: 69  EVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNVENNQSF 128
           EVT+  FDSC     L  ++ GN+ V L+ PG+R+F+ G   +C  GMKL+VNV    + 
Sbjct: 68  EVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNVVPTATV 127

Query: 129 SPAAAPQPN---GTALPRPSS 146
           +P  AP PN       P PSS
Sbjct: 128 AP-TAPLPNTVPSLNAPSPSS 147

[Close]

BLAST of MS017950.1 vs. TAIR 10
Match: AT3G27200.1 (Cupredoxin superfamily protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.8e-17
Identity = 48/142 (33.80%), Postives = 81/142 (57.04%), Query Frame = 0


Query: 18  ALRSCTATTYIVGDSSGWDISTDLDTWSQGKRFFVGDLLVFQYSSSESLNEVTREN-FDS 77
           ++++  A  +++G S GW+ S D D+WS  + F VGD +VF+YS   S+ E+  E  + S
Sbjct: 17  SVKTALAARHVIGGSQGWEQSVDFDSWSSDQSFKVGDQIVFKYSELHSVVELGSETAYKS 76

Query: 78  CNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNVENNQSFSPAAAPQPN 137
           C+    + + S+GN  V LS+ G R+F  G   +C  GMK++VNV ++ S S A++P  +
Sbjct: 77  CDLGTSVNSLSSGNDVVKLSKTGTRYFACGTVGHCEQGMKIKVNVVSSDSKS-ASSPSGS 136

Query: 138 GTALPRPSSKTDDTVPGASAAS 159
           G+     S     +  G  A++
Sbjct: 137 GSGSDSGSGSGSSSGHGLRAST 157

[Close]

BLAST of MS017950.1 vs. TAIR 10
Match: AT4G31840.1 (early nodulin-like protein 15 )

HSP 1 Score: 84.3 bits (207), Expect = 1.1e-16
Identity = 59/165 (35.76%), Postives = 85/165 (51.52%), Query Frame = 0


Query: 7   LHVFFMIIAGFALRSCTATTYIVGDSSG-WDI----STDLDTWSQGKRFFVGDLLVFQY- 66
           L   F+ I+ F   S  A    VG  SG W I    S   + W+Q  RF VGD +VF+Y 
Sbjct: 7   LVTIFLCISVFFFSSVNANEVTVGGKSGDWKIPPSSSFSFNEWAQKARFKVGDFIVFKYE 66

Query: 67  SSSESLNEVTRENFDSCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVN 126
           +  +S+ +VTRE ++ CNT +   +Y+ GNT V L + G  +FVSG   +C  G KL++ 
Sbjct: 67  AGKDSVLQVTREAYEKCNTTSPKASYTDGNTKVKLDQAGPVYFVSGTEGHCQKGQKLRLV 126

Query: 127 V--ENNQSFSPAAAPQP-NGTALPRPSSKTDDTVPGASAASIIGG 163
           V    N +FSP  +P   +G A+           P + AA + GG
Sbjct: 127 VITPRNSAFSPGPSPSEFDGPAV----------APTSGAAKLAGG 161

[Close]

BLAST of MS017950.1 vs. TAIR 10
Match: AT5G26330.1 (Cupredoxin superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 2.4e-16
Identity = 55/170 (32.35%), Postives = 89/170 (52.35%), Query Frame = 0


Query: 19  LRSCTATTYIVGDSSGWDISTDLD--TWSQGKRFFVGDLLVFQYSSS-ESLNEVTRENFD 78
           LR   A  Y VGDS+GW    ++D   W+  K F +GD ++F+Y+    ++  VT   + 
Sbjct: 16  LRLSEAAVYKVGDSAGWTTIANVDYKLWASTKTFHIGDTVLFEYNPQFHNVMRVTHPMYR 75

Query: 79  SCNTAAVLKAYSTGNTTVTLSEPGQRFFVSGNRLYCLGGMKLQVNVENNQSFSPAAAPQP 138
           SCNT+  +  ++TGN ++TL+  G  FF  G   +CL G KL ++V    S +P + P  
Sbjct: 76  SCNTSKPISTFTTGNDSITLTNHGHHFFFCGVPGHCLAGQKLDLHVLLPASSTPLSDPPT 135

Query: 139 NGTALPRPSSKTDDTVPGAS---AASIIGGREALVLAFVCYVISTLMVVF 183
           + ++ P  ++     VPG S   AAS+     A ++A V  ++S     F
Sbjct: 136 SSSSSPPSTTIPAAGVPGPSPSLAASLPSMVTAQIVAVVTLLVSLAFTNF 185

The following BLAST results are available for this feature:

BLAST of MS017950.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877141.1	3.3e-68	75.54	stellacyanin-like [Benincasa hispida]	[more]
XP_011654854.1	1.8e-66	74.46	blue copper protein [Cucumis sativus] >KGN50322.1 hypothetical protein Csa_00051...	[more]
XP_023549499.1	2.9e-64	72.93	stellacyanin-like [Cucurbita pepo subsp. pepo]	[more]
XP_008436972.1	1.4e-63	72.53	PREDICTED: stellacyanin [Cucumis melo]	[more]
XP_022969745.1	4.2e-63	71.67	stellacyanin [Cucurbita maxima]	[more]

BLAST of MS017950.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82081	2.5e-18	37.59	Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1	[more]
Q02917	1.8e-13	32.39	Early nodulin-55-2 OS=Glycine max OX=3847 GN=ENOD55-2 PE=2 SV=1	[more]
Q9SK27	1.2e-12	38.00	Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 S...	[more]
Q8LC95	1.2e-12	32.39	Early nodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g25090 PE=2 S...	[more]
A0A0M4FTF3	1.3e-11	33.62	Blue copper protein OS=Petunia hybrida OX=4102 GN=BCP PE=2 SV=1	[more]

BLAST of MS017950.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPG5	8.8e-67	74.46	Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G167140...	[more]
A0A1S3AT20	6.9e-64	72.53	stellacyanin OS=Cucumis melo OX=3656 GN=LOC103482542 PE=4 SV=1	[more]
A0A6J1I0T8	2.0e-63	71.67	stellacyanin OS=Cucurbita maxima OX=3661 GN=LOC111468850 PE=4 SV=1	[more]
A0A6J1ERB5	7.7e-63	75.00	stellacyanin OS=Cucurbita moschata OX=3662 GN=LOC111435827 PE=4 SV=1	[more]
A0A5A7UGX7	5.0e-62	72.47	Stellacyanin OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00080 P...	[more]

BLAST of MS017950.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07475.1	3.4e-39	50.98	Cupredoxin superfamily protein	[more]
AT2G32300.1	1.8e-19	37.59	uclacyanin 1	[more]
AT3G27200.1	2.8e-17	33.80	Cupredoxin superfamily protein	[more]
AT4G31840.1	1.1e-16	35.76	early nodulin-like protein 15	[more]
AT5G26330.1	2.4e-16	32.35	Cupredoxin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 23..128 e-value: 1.3E-30 score: 107.7
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 24..121
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 36..115 e-value: 1.8E-20 score: 72.7
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 25..123 score: 34.646088
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 126..153
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 126..152
None	No IPR available	PANTHER	PTHR33021:SF70	OSJNBA0079A21.6 PROTEIN	coord: 7..175
None	No IPR available	CDD	cd04216	Phytocyanin	coord: 25..121 e-value: 2.85022E-41 score: 132.769
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 7..175

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS017950	MS017950	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS017950.1-cds	MS017950.1-cds-scaffold373:4084980..4085148	CDS
MS017950.1-cds	MS017950.1-cds-scaffold373:4086201..4086577	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS017950.1	MS017950.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0009055	electron transfer activity