MS014185.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS014185.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	pentatricopeptide repeat-containing protein At2g30100, chloroplastic
Location	scaffold5: 1272153 .. 1274780 (-)
Sequence length	1533
RNA-Seq Expression	MS014185.1
Synteny	MS014185.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTGTGCCCAGGGCTTTACTCCGGTGACTCAATTTGGTTTCTCGTTTTCTTTATCTTCTGCACTGAAAACTCAGAGGCAAAGATTCTTCTCCACTCCCCAATTGTATTGCCACTCGCCGGTAAATTTTTGCTTTATGATTTCTTGCATTACCTGCAACCACCGGAATTCTACCTTCTCTGTTCTGAAAGCCGGTAAGTTTCGGGACCTGAGGTTGTTCAAATCGGTTGAGTTGGACCAGTTCATCACGAGTGACGACGAAGACGAAATGGGAGATGGATTTTTTGAGGCAATCGAGGAGTTGGAGCGCATGACGAGGGAACCATCGGATGTTCTCGAAGAAATGAATGACCGCCTTTCAGCGAGGGAATTCCAGCTTGTGCTTGTGTACTTCTCTCAAGAAGGGAGAGATTCATGGTGTGCTCTTGAGGTTTTTGAGTGGCTCCAGAAAGAAAATCGGGTCGACAAGGAGACCATGGAGCTGATGGTGTCTATTATGTGCAGTTGGATTAAAAAATTGGTTGAGGGAGACCATAACGTCGGAGATGTGGTTGACCTTCTCGTAGATATGGATTGTGTAGGTTTGAAACCTCATTTTAGCATGATAGAAAAAGTCATTTCCTTGTATTGGGAAATGGGCGAGAAGGAACAAGCTATTTCGTTCGTGAAAGAGGTCTTGGGACGCAAGATTGCTTTTATGAAGGACGATCGGGAGGGGCATAAAGGGGGACCAAGCGGTTATCTCGCATGGAAGATGATGGTAAGCCTTCTTCTAATCGCCATTTTCATATTTTAAGCCATTGACCTTGCTTAAAAGACAATATCAGTATTATTTTGCTGTGTCTATCTATTACATCAATAAATCGGAAATGAAATGTAAGTTTAGCACTTCGATCATTGTTTACTACACTTCAGGTGTCGTATTTTGAATCCTGAAATGCCTAATCTTAGAGATTCCTTACTCTGTCTATTTTACTCCTCTAGTACTCATTTAGGTTAGTATCTTAATCTTATTGGATTTTGATTATGCACTCTATATTCAGCATCGGGTTTTTGTTGTGATGGCTTTGGTCAAGGTGGTAAGGGATATTTTTTGGCGTTGTGTTTGTCTAATTGCAATTGAAGGGCTCCTGGAAGAACTGTGCAGGTCTATTCATCAGAGAGCTAGATGGGGCATTCAGGGTATTATGGTTTAACAGTAATTTTGAAATTTATGGTTAAGCTGCGTTCTATTGTGGTTAAGCATGTTTGAATCTATTGTTGTGTTTGAGTGTTGGACAAACTCAGTCAGTTATGTTGTTAGTGAAGTTTATTTCTGCAGAGGTAGGATTTGGAAAAACAATGAAATCTTGTTTAGGGTGTTTTTTTCCCTACCAGAACTATGGTGTCATTGGTTTCACTCATTGAAAACTTTAGGGCATGTCTTTGTTCATTGTAATTTTCTTTGGATCTTAGTTGTCGTTACTCTCCCATTAGTCAGAGTATTTGTATTTCTTGCCTCTTGTTCAATTTTTTTGGTCGTCGAATTGCCTCTCTCAATGTCATCATTTATGTTGACTGTTGTATTGGACTTCCATTTCATATGCATCACTACTACTATTCTCTTGTTTGGATTAGTGGCACACTCCTATGGTTTCATTCATATTCGAAAAACATTGAGCTGTATAAGACCGAGTTAATCCAATCTCGTGACTATATTAGCACACTGCTGTAATTCTTCTTCTCTTAACCAGCAATGGTTGTGGATTAGCTGCCCAAGGATTTTAAATTACTACTTTCAAATTGTTATGTTTGTTCAGCACAACCCCAAGTGAAAAATTCAATTGCTTATGATCTTGAAAATCAATTCTCTTAGGTTGATGGTGACTATAGGGGTGCAGTGAAATTGGTGCTGCATCTTAGAGAATCTGGATTAAATCCAGAGGTTTATAGCTACCTCATCGCCATGACTGCTGTGGTTAAAGAGCTGAATGAATTTGCAAAAGCTCTACGCAAACTCAAAAGTTACACAAGAGATGGAATAGTAGCCGAACTTGATAAAGACAATGTTGGACTTGTGGAGAGATATCAGACAGAGCTTCTAGCCGATGGAGTACGATTATCCAACTGGGTGCTTGAAGAGGGAAGCTCTTCAATTCATGGGGTGGCTCATGAGAGACTTCTTGCTATGTATATTTGCGCCGGGCGAGGACTTGAGGCAGAGAGACAACTTTGGGAAATGAAGCTTGTAGGTAAGGAGGCTGATAGTGATCTCTATGATATCGTGCTAGCCATCTGTGCTTCACAGAAGGAGACTAGAGCAATGAACCGGTTGCTTACCAGGATTGAGATTATAAGTCCCCTGCTGAAGAAGAAGAGCCTATCATGGCTGCTAAGGGGTTACATAAAAGGAGGGCATTTCAGTGATGCTGCAGAAACATTAGTAAAAATGGTTGATTTGGGTTTTCTCCCAGAATACTTGGACAGAGTAGCTGTGCTGCAAGGCCTAAGAAAACGGATTCGGGAACCTGGAAGTGTGGAAACTTACTTCAAGCTCTGCAAGTGTCTCTCAGATGCTAATCTGATTGGACCTAGTCTTGTATATTTGCACTTACAGAAACACAAGCTTTGGGTCATTAAAATGCTT

mRNA sequence

ATGATTTGTGCCCAGGGCTTTACTCCGGTGACTCAATTTGGTTTCTCGTTTTCTTTATCTTCTGCACTGAAAACTCAGAGGCAAAGATTCTTCTCCACTCCCCAATTGTATTGCCACTCGCCGGTAAATTTTTGCTTTATGATTTCTTGCATTACCTGCAACCACCGGAATTCTACCTTCTCTGTTCTGAAAGCCGGTAAGTTTCGGGACCTGAGGTTGTTCAAATCGGTTGAGTTGGACCAGTTCATCACGAGTGACGACGAAGACGAAATGGGAGATGGATTTTTTGAGGCAATCGAGGAGTTGGAGCGCATGACGAGGGAACCATCGGATGTTCTCGAAGAAATGAATGACCGCCTTTCAGCGAGGGAATTCCAGCTTGTGCTTGTGTACTTCTCTCAAGAAGGGAGAGATTCATGGTGTGCTCTTGAGGTTTTTGAGTGGCTCCAGAAAGAAAATCGGGTCGACAAGGAGACCATGGAGCTGATGGTGTCTATTATGTGCAGTTGGATTAAAAAATTGGTTGAGGGAGACCATAACGTCGGAGATGTGGTTGACCTTCTCGTAGATATGGATTGTGTAGGTTTGAAACCTCATTTTAGCATGATAGAAAAAGTCATTTCCTTGTATTGGGAAATGGGCGAGAAGGAACAAGCTATTTCGTTCGTGAAAGAGGTCTTGGGACGCAAGATTGCTTTTATGAAGGACGATCGGGAGGGGCATAAAGGGGGACCAAGCGGTTATCTCGCATGGAAGATGATGGTTGATGGTGACTATAGGGGTGCAGTGAAATTGGTGCTGCATCTTAGAGAATCTGGATTAAATCCAGAGGTTTATAGCTACCTCATCGCCATGACTGCTGTGGTTAAAGAGCTGAATGAATTTGCAAAAGCTCTACGCAAACTCAAAAGTTACACAAGAGATGGAATAGTAGCCGAACTTGATAAAGACAATGTTGGACTTGTGGAGAGATATCAGACAGAGCTTCTAGCCGATGGAGTACGATTATCCAACTGGGTGCTTGAAGAGGGAAGCTCTTCAATTCATGGGGTGGCTCATGAGAGACTTCTTGCTATGTATATTTGCGCCGGGCGAGGACTTGAGGCAGAGAGACAACTTTGGGAAATGAAGCTTGTAGGTAAGGAGGCTGATAGTGATCTCTATGATATCGTGCTAGCCATCTGTGCTTCACAGAAGGAGACTAGAGCAATGAACCGGTTGCTTACCAGGATTGAGATTATAAGTCCCCTGCTGAAGAAGAAGAGCCTATCATGGCTGCTAAGGGGTTACATAAAAGGAGGGCATTTCAGTGATGCTGCAGAAACATTAGTAAAAATGGTTGATTTGGGTTTTCTCCCAGAATACTTGGACAGAGTAGCTGTGCTGCAAGGCCTAAGAAAACGGATTCGGGAACCTGGAAGTGTGGAAACTTACTTCAAGCTCTGCAAGTGTCTCTCAGATGCTAATCTGATTGGACCTAGTCTTGTATATTTGCACTTACAGAAACACAAGCTTTGGGTCATTAAAATGCTT

Coding sequence (CDS)

ATGATTTGTGCCCAGGGCTTTACTCCGGTGACTCAATTTGGTTTCTCGTTTTCTTTATCTTCTGCACTGAAAACTCAGAGGCAAAGATTCTTCTCCACTCCCCAATTGTATTGCCACTCGCCGGTAAATTTTTGCTTTATGATTTCTTGCATTACCTGCAACCACCGGAATTCTACCTTCTCTGTTCTGAAAGCCGGTAAGTTTCGGGACCTGAGGTTGTTCAAATCGGTTGAGTTGGACCAGTTCATCACGAGTGACGACGAAGACGAAATGGGAGATGGATTTTTTGAGGCAATCGAGGAGTTGGAGCGCATGACGAGGGAACCATCGGATGTTCTCGAAGAAATGAATGACCGCCTTTCAGCGAGGGAATTCCAGCTTGTGCTTGTGTACTTCTCTCAAGAAGGGAGAGATTCATGGTGTGCTCTTGAGGTTTTTGAGTGGCTCCAGAAAGAAAATCGGGTCGACAAGGAGACCATGGAGCTGATGGTGTCTATTATGTGCAGTTGGATTAAAAAATTGGTTGAGGGAGACCATAACGTCGGAGATGTGGTTGACCTTCTCGTAGATATGGATTGTGTAGGTTTGAAACCTCATTTTAGCATGATAGAAAAAGTCATTTCCTTGTATTGGGAAATGGGCGAGAAGGAACAAGCTATTTCGTTCGTGAAAGAGGTCTTGGGACGCAAGATTGCTTTTATGAAGGACGATCGGGAGGGGCATAAAGGGGGACCAAGCGGTTATCTCGCATGGAAGATGATGGTTGATGGTGACTATAGGGGTGCAGTGAAATTGGTGCTGCATCTTAGAGAATCTGGATTAAATCCAGAGGTTTATAGCTACCTCATCGCCATGACTGCTGTGGTTAAAGAGCTGAATGAATTTGCAAAAGCTCTACGCAAACTCAAAAGTTACACAAGAGATGGAATAGTAGCCGAACTTGATAAAGACAATGTTGGACTTGTGGAGAGATATCAGACAGAGCTTCTAGCCGATGGAGTACGATTATCCAACTGGGTGCTTGAAGAGGGAAGCTCTTCAATTCATGGGGTGGCTCATGAGAGACTTCTTGCTATGTATATTTGCGCCGGGCGAGGACTTGAGGCAGAGAGACAACTTTGGGAAATGAAGCTTGTAGGTAAGGAGGCTGATAGTGATCTCTATGATATCGTGCTAGCCATCTGTGCTTCACAGAAGGAGACTAGAGCAATGAACCGGTTGCTTACCAGGATTGAGATTATAAGTCCCCTGCTGAAGAAGAAGAGCCTATCATGGCTGCTAAGGGGTTACATAAAAGGAGGGCATTTCAGTGATGCTGCAGAAACATTAGTAAAAATGGTTGATTTGGGTTTTCTCCCAGAATACTTGGACAGAGTAGCTGTGCTGCAAGGCCTAAGAAAACGGATTCGGGAACCTGGAAGTGTGGAAACTTACTTCAAGCTCTGCAAGTGTCTCTCAGATGCTAATCTGATTGGACCTAGTCTTGTATATTTGCACTTACAGAAACACAAGCTTTGGGTCATTAAAATGCTT

Protein sequence

MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTFSVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRLSAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHNVGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREGHKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALRKLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMYICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKKKSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFKLCKCLSDANLIGPSLVYLHLQKHKLWVIKML

Homology

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BLAST of MS014185.1 vs. NCBI nr
Match: XP_022148369.1 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Momordica charantia])

HSP 1 Score: 1019.6 bits (2635), Expect = 9.3e-294
Identity = 509/511 (99.61%), Postives = 509/511 (99.61%), Query Frame = 0


Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF
Sbjct: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL
Sbjct: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSYTRDGIVAELDKDNVGLVE YQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY
Sbjct: 301 KLKSYTRDGIVAELDKDNVGLVEIYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK
Sbjct: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK
Sbjct: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGP LVYLHLQKHKLWVIKML
Sbjct: 481 LCKCLSDANLIGPGLVYLHLQKHKLWVIKML 511

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BLAST of MS014185.1 vs. NCBI nr
Match: XP_038901728.1 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Benincasa hispida])

HSP 1 Score: 928.7 bits (2399), Expect = 2.2e-266
Identity = 462/511 (90.41%), Postives = 485/511 (94.91%), Query Frame = 0


Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           M+CAQGFTP+TQFGFSFSLSSALKT R   FSTPQLY   PV FCFM+S I+CN+++STF
Sbjct: 1   MVCAQGFTPLTQFGFSFSLSSALKTDRHG-FSTPQLYSPPPVKFCFMVSRISCNYQDSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +AGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL
Sbjct: 61  SVSRAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGQHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKE+AISFVKEVLGR +AFMKDD EG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEKAISFVKEVLGRNLAFMKDDWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VLHLRESGL PEVY YLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLHLRESGLKPEVYCYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDGIVAELDK+NV LVE+YQTELLADGVRLSNWVLEEGS SIHGV HERLLAMY
Sbjct: 301 KLKSYARDGIVAELDKNNVELVEKYQTELLADGVRLSNWVLEEGSFSIHGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+GLEAERQLWEMKLVGKEAD+DLYDIVLAICASQKET+AM RLLTRIEI SP+ KK
Sbjct: 361 ICAGQGLEAERQLWEMKLVGKEADADLYDIVLAICASQKETKAMKRLLTRIEITSPMRKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAAETLVKM+DLGFLPEYLDRVAVLQGLRK+IREP +V+TY  
Sbjct: 421 KSLTWLLRGYIKGGHFHDAAETLVKMIDLGFLPEYLDRVAVLQGLRKQIREPENVDTYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQKHKLWV+KML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVVKML 510

[Close]

BLAST of MS014185.1 vs. NCBI nr
Match: KAG7010495.1 (Pentatricopeptide repeat-containing protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 922.2 bits (2382), Expect = 2.0e-264
Identity = 461/511 (90.22%), Postives = 485/511 (94.91%), Query Frame = 0


Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGF+P+TQFGFSFSLSS LK++R   FS PQL   SPVNFCFM+S ITCNH+NSTF
Sbjct: 1   MICAQGFSPLTQFGFSFSLSSGLKSERLG-FSAPQLCSRSPVNFCFMVSRITCNHQNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +AGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTR+PSDVLEEMNDRL
Sbjct: 61  SVSRAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTRDPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGQHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYW+MGEKE+AISFVKEVLGRK+ FMKD+ EG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWDMGEKEKAISFVKEVLGRKLDFMKDNWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VL+LRESGL PEVY YLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLNLRESGLKPEVYCYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDG+VAELDKDNV LV+RYQ+ELLADGVRLSNWVL+EG SS HGV HERLLAMY
Sbjct: 301 KLKSYARDGMVAELDKDNVELVKRYQSELLADGVRLSNWVLDEGGSSSHGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+GLEAERQLWEMKLVGKEAD+DLYDIVLAICASQKETRAMNRLLTRIEI SP LKK
Sbjct: 361 ICAGQGLEAERQLWEMKLVGKEADADLYDIVLAICASQKETRAMNRLLTRIEITSPRLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAAETLVKMV+LGFLPEYLDRVAVLQGLRKRIREP +VETY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAETLVKMVNLGFLPEYLDRVAVLQGLRKRIREPENVETYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQK+KLWVIKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKYKLWVIKML 510

[Close]

BLAST of MS014185.1 vs. NCBI nr
Match: XP_023512972.1 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 920.6 bits (2378), Expect = 5.9e-264
Identity = 461/511 (90.22%), Postives = 484/511 (94.72%), Query Frame = 0


Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTP+TQFGFSFSLSS LK++R   FS PQL   SPVNFCFM+S ITCNH+NSTF
Sbjct: 1   MICAQGFTPLTQFGFSFSLSSGLKSERLG-FSAPQLCSRSPVNFCFMVSRITCNHQNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +AGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTR+PSDVLEEMNDRL
Sbjct: 61  SVSRAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTRDPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGQHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYW+MGEKE+AISFVKEVLGRK+ FMKD+ EG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWDMGEKEKAISFVKEVLGRKLDFMKDNWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VL+LRESGL PEVY YLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLNLRESGLKPEVYCYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDG+VAELDKDNV LV+RYQ+ELLADGVRLSNWVL+EG SS H V HERLLAMY
Sbjct: 301 KLKSYARDGMVAELDKDNVELVKRYQSELLADGVRLSNWVLDEGGSSSHRVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+GLEAERQLWEMKLVGKEAD+DLYDIVLAICASQKETRAMNRLLTRIEI SP LKK
Sbjct: 361 ICAGQGLEAERQLWEMKLVGKEADADLYDIVLAICASQKETRAMNRLLTRIEITSPRLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAAETLVKMV+LGFLPEYLDRVAVLQGLRKRIREP +VETY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAETLVKMVNLGFLPEYLDRVAVLQGLRKRIREPENVETYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQK+KLWVIKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKYKLWVIKML 510

[Close]

BLAST of MS014185.1 vs. NCBI nr
Match: XP_022944005.1 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Cucurbita moschata])

HSP 1 Score: 920.6 bits (2378), Expect = 5.9e-264
Identity = 461/511 (90.22%), Postives = 484/511 (94.72%), Query Frame = 0


Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTP+TQFGFSFSLSS LK++R   FS PQL   SPVNFCFM+S ITCNH+NSTF
Sbjct: 1   MICAQGFTPLTQFGFSFSLSSGLKSERLG-FSAPQLCSRSPVNFCFMVSRITCNHQNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +AGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTR+PSDVLEEMNDRL
Sbjct: 61  SVSRAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTRDPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGQHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           V DVVDLLVDMDCVGLKPHFSMIEKVISLYW+MGEKE+AISFVKEVLGRK+ FMKD+ EG
Sbjct: 181 VRDVVDLLVDMDCVGLKPHFSMIEKVISLYWDMGEKEKAISFVKEVLGRKLDFMKDNWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VL+LRESGL PEVY YLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLNLRESGLKPEVYCYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDG+VAELDKDNV LV+RYQ+ELLADGVRLSNWVL+EG SS HGV HERLLAMY
Sbjct: 301 KLKSYARDGMVAELDKDNVELVKRYQSELLADGVRLSNWVLDEGGSSSHGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+GLEAERQLWEMKLVGKEAD+DLYDIVLAICASQKETRAMNRLLTRIEI SP LKK
Sbjct: 361 ICAGQGLEAERQLWEMKLVGKEADADLYDIVLAICASQKETRAMNRLLTRIEITSPRLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAAETLVKMV+LGFLPEYLDRVAVLQGLRKRIREP +VETY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAETLVKMVNLGFLPEYLDRVAVLQGLRKRIREPENVETYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQK+KLWVIKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKYKLWVIKML 510

[Close]

BLAST of MS014185.1 vs. ExPASy Swiss-Prot
Match: Q0WNN7 (Pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g30100 PE=2 SV=2)

HSP 1 Score: 604.7 bits (1558), Expect = 9.3e-172
Identity = 297/458 (64.85%), Postives = 367/458 (80.13%), Query Frame = 0


Query: 65  AGKFRDLRLFKSVELDQFITSDDE----DEMGDGFFEAIEELERMTREPSDVLEEMNDRL 124
           AGKFR++ L +SVELDQFITS++E    +E+G+GFFEAIEELERMTREPSD+LEEMN RL
Sbjct: 46  AGKFREMGLSRSVELDQFITSEEEEGEAEEIGEGFFEAIEELERMTREPSDILEEMNHRL 105

Query: 125 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 184
           S+RE QL+LVYF+QEGRDSWC LEVFEWL+KENRVD+E MELMVSIMC W+KKL+E + N
Sbjct: 106 SSRELQLMLVYFAQEGRDSWCTLEVFEWLKKENRVDEEIMELMVSIMCGWVKKLIEDECN 165

Query: 185 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKD---- 244
              V DLL++MDCVGLKP FSM++KVI+LY EMG+KE A+ FVKEVL R+  F       
Sbjct: 166 AHQVFDLLIEMDCVGLKPGFSMMDKVIALYCEMGKKESAVLFVKEVLRRRDGFGYSVVGG 225

Query: 245 -DREGHKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEF 304
              EG KGGP GYLAWK MVDGDYR AV +V+ LR SGL PE YSYLIAMTA+VKELN  
Sbjct: 226 GGSEGRKGGPVGYLAWKFMVDGDYRKAVDMVMELRLSGLKPEAYSYLIAMTAIVKELNSL 285

Query: 305 AKALRKLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEG--SSSIHGVAH 364
            K LR+LK + R G VAE+D  +  L+E+YQ+E L+ G++L+ W +EEG  + SI GV H
Sbjct: 286 GKTLRELKRFARAGFVAEIDDHDRVLIEKYQSETLSRGLQLATWAVEEGQENDSIIGVVH 345

Query: 365 ERLLAMYICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEI 424
           ERLLAMYICAGRG EAE+QLW+MKL G+E ++DL+DIV+AICASQKE  A++RLLTR+E 
Sbjct: 346 ERLLAMYICAGRGPEAEKQLWKMKLAGREPEADLHDIVMAICASQKEVNAVSRLLTRVEF 405

Query: 425 ISPLLKKKSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPG 484
           +    KKK+LSWLLRGY+KGGHF +AAETLV M+D G  PEY+DRVAV+QG+ ++I+ P 
Sbjct: 406 MGSQRKKKTLSWLLRGYVKGGHFEEAAETLVSMIDSGLHPEYIDRVAVMQGMTRKIQRPR 465

Query: 485 SVETYFKLCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
            VE Y  LCK L DA L+GP LVY+++ K+KLW++KM+
Sbjct: 466 DVEAYMSLCKRLFDAGLVGPCLVYMYIDKYKLWIVKMM 503

[Close]

BLAST of MS014185.1 vs. ExPASy Swiss-Prot
Match: Q0WVV0 (Pentatricopeptide repeat-containing protein At1g10910, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g10910 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.2e-06
Identity = 45/215 (20.93%), Postives = 87/215 (40.47%), Query Frame = 0


Query: 253 MMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALRKLKSYTRDGIVA 312
           ++ +G     +KL   ++  GL P+V +Y   +   +K  N + KA+  +     +GI  
Sbjct: 176 LVKNGKLDSCIKLFDQMKRDGLKPDVVTYNTLLAGCIKVKNGYPKAIELIGELPHNGI-- 235

Query: 313 ELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMYICAGRGLEAERQ 372
                                              +  V +  +LA+    GR  EAE  
Sbjct: 236 ----------------------------------QMDSVMYGTVLAICASNGRSEEAENF 295

Query: 373 LWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKKKSLSWLLRGYIK 432
           + +MK+ G   +   Y  +L   + + + +  + L+T ++ I  +  K  ++ LL+ YIK
Sbjct: 296 IQQMKVEGHSPNIYHYSSLLNSYSWKGDYKKADELMTEMKSIGLVPNKVMMTTLLKVYIK 354

Query: 433 GGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRK 468
           GG F  + E L ++   G+    +    ++ GL K
Sbjct: 356 GGLFDRSRELLSELESAGYAENEMPYCMLMDGLSK 354

[Close]

BLAST of MS014185.1 vs. ExPASy TrEMBL
Match: A0A6J1D3T2 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111017036 PE=4 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 4.5e-294
Identity = 509/511 (99.61%), Postives = 509/511 (99.61%), Query Frame = 0


Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF
Sbjct: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL
Sbjct: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSYTRDGIVAELDKDNVGLVE YQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY
Sbjct: 301 KLKSYTRDGIVAELDKDNVGLVEIYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK
Sbjct: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK
Sbjct: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGP LVYLHLQKHKLWVIKML
Sbjct: 481 LCKCLSDANLIGPGLVYLHLQKHKLWVIKML 511

[Close]

BLAST of MS014185.1 vs. ExPASy TrEMBL
Match: A0A6J1FYE9 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448564 PE=4 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 2.8e-264
Identity = 461/511 (90.22%), Postives = 484/511 (94.72%), Query Frame = 0


Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTP+TQFGFSFSLSS LK++R   FS PQL   SPVNFCFM+S ITCNH+NSTF
Sbjct: 1   MICAQGFTPLTQFGFSFSLSSGLKSERLG-FSAPQLCSRSPVNFCFMVSRITCNHQNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +AGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTR+PSDVLEEMNDRL
Sbjct: 61  SVSRAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTRDPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGQHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           V DVVDLLVDMDCVGLKPHFSMIEKVISLYW+MGEKE+AISFVKEVLGRK+ FMKD+ EG
Sbjct: 181 VRDVVDLLVDMDCVGLKPHFSMIEKVISLYWDMGEKEKAISFVKEVLGRKLDFMKDNWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VL+LRESGL PEVY YLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLNLRESGLKPEVYCYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDG+VAELDKDNV LV+RYQ+ELLADGVRLSNWVL+EG SS HGV HERLLAMY
Sbjct: 301 KLKSYARDGMVAELDKDNVELVKRYQSELLADGVRLSNWVLDEGGSSSHGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+GLEAERQLWEMKLVGKEAD+DLYDIVLAICASQKETRAMNRLLTRIEI SP LKK
Sbjct: 361 ICAGQGLEAERQLWEMKLVGKEADADLYDIVLAICASQKETRAMNRLLTRIEITSPRLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAAETLVKMV+LGFLPEYLDRVAVLQGLRKRIREP +VETY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAETLVKMVNLGFLPEYLDRVAVLQGLRKRIREPENVETYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQK+KLWVIKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKYKLWVIKML 510

[Close]

BLAST of MS014185.1 vs. ExPASy TrEMBL
Match: A0A6J1JH85 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484464 PE=4 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.4e-263
Identity = 458/511 (89.63%), Postives = 485/511 (94.91%), Query Frame = 0


Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICA GFTP+T+FGFSFSLSS LK++R   FS PQL   SPVNFCF++S ITCNH+NSTF
Sbjct: 1   MICAPGFTPLTKFGFSFSLSSGLKSKRLG-FSAPQLCSRSPVNFCFIVSRITCNHQNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +AGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTR+PSDVLEEMNDRL
Sbjct: 61  SVSRAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTRDPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGQHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYW+MGEKE+AISFVKEVLGRK+ FMKD+ EG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWDMGEKEKAISFVKEVLGRKLDFMKDNWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VL+LRESGL PEVY +LIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLNLRESGLKPEVYCFLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDG+VAELDKDNV LV+RYQ+ELLADGVRLSNWVL+EGSSS HGV HERLLAMY
Sbjct: 301 KLKSYARDGMVAELDKDNVELVKRYQSELLADGVRLSNWVLDEGSSSSHGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+GLEAERQLWEMKLVGKEAD+DLYDIVLAICASQKETRAMNRLL+RIEI SP LKK
Sbjct: 361 ICAGQGLEAERQLWEMKLVGKEADADLYDIVLAICASQKETRAMNRLLSRIEITSPRLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAAETLVKMV+LGFLPEYLDRVAVLQGLRKRIREP +VETY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAETLVKMVNLGFLPEYLDRVAVLQGLRKRIREPENVETYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQK+KLWVIKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKYKLWVIKML 510

[Close]

BLAST of MS014185.1 vs. ExPASy TrEMBL
Match: A0A0A0KC35 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G182120 PE=4 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.5e-254
Identity = 446/511 (87.28%), Postives = 473/511 (92.56%), Query Frame = 0


Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTP+TQFGFSFSLSS L++QR   FSTP+LY         M+S I+CN+++STF
Sbjct: 1   MICAQGFTPLTQFGFSFSLSSPLESQRCG-FSTPRLY---------MVSPISCNYQDSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +A KFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL
Sbjct: 61  SVSRAAKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SARE QLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREIQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGRHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKE+A+ FVKEVLGR +AFMKDD EG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEKAVFFVKEVLGRNLAFMKDDWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VLHLRESGL PEVYSYLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLHLRESGLRPEVYSYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLK Y RDG VAELDK+NV LV +YQTELLADGV+LSNWVLEEGSSSI GV HERLLAMY
Sbjct: 301 KLKGYARDGFVAELDKNNVELVAKYQTELLADGVQLSNWVLEEGSSSIRGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+G+EAERQLWEMKLVGKEAD+DLYDIVLAICASQKET+AM RLLTRIEI SP++KK
Sbjct: 361 ICAGQGVEAERQLWEMKLVGKEADADLYDIVLAICASQKETKAMKRLLTRIEITSPMIKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAA TLVKM++LGFLPEYLDRVAVLQGLRK IREP SV TY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAGTLVKMINLGFLPEYLDRVAVLQGLRKEIREPESVHTYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQKHKLW+IKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKHKLWIIKML 501

[Close]

BLAST of MS014185.1 vs. ExPASy TrEMBL
Match: A0A1S3CNE0 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502924 PE=4 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 7.7e-254
Identity = 446/511 (87.28%), Postives = 472/511 (92.37%), Query Frame = 0


Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTP+TQFGFSFSLSS L+TQR   FSTP+LY         M+S I+CN+++STF
Sbjct: 1   MICAQGFTPLTQFGFSFSLSSPLETQRYG-FSTPRLY---------MVSPISCNYQDSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +A KFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL
Sbjct: 61  SVSRAAKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SARE QLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWI KLVEG HN
Sbjct: 121 SAREIQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWINKLVEGRHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKE+AI FVKEVLGR +AFMKDD EG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEKAIFFVKEVLGRNLAFMKDDWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VLHLRESGL PEVYSYLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLHLRESGLRPEVYSYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDG VAELDK+NV LV +YQTELLADGVRLSNWVLEEGSSSIHGV HERLLAMY
Sbjct: 301 KLKSYARDGYVAELDKNNVELVAKYQTELLADGVRLSNWVLEEGSSSIHGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+G+EAERQLWEMKL+GKEAD+DLYDIVLAICASQKE +AM RLLTRIEI SP++KK
Sbjct: 361 ICAGQGVEAERQLWEMKLLGKEADADLYDIVLAICASQKEIKAMKRLLTRIEITSPMIKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAA T+VKM++LGFLPEYLDRVAVLQGLRK IREP  V TY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAGTVVKMINLGFLPEYLDRVAVLQGLRKGIREPEIVHTYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQKHKLW+IKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKHKLWIIKML 501

[Close]

BLAST of MS014185.1 vs. TAIR 10
Match: AT2G30100.1 (pentatricopeptide (PPR) repeat-containing protein )

HSP 1 Score: 604.7 bits (1558), Expect = 6.6e-173
Identity = 297/458 (64.85%), Postives = 367/458 (80.13%), Query Frame = 0


Query: 65  AGKFRDLRLFKSVELDQFITSDDE----DEMGDGFFEAIEELERMTREPSDVLEEMNDRL 124
           AGKFR++ L +SVELDQFITS++E    +E+G+GFFEAIEELERMTREPSD+LEEMN RL
Sbjct: 46  AGKFREMGLSRSVELDQFITSEEEEGEAEEIGEGFFEAIEELERMTREPSDILEEMNHRL 105

Query: 125 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 184
           S+RE QL+LVYF+QEGRDSWC LEVFEWL+KENRVD+E MELMVSIMC W+KKL+E + N
Sbjct: 106 SSRELQLMLVYFAQEGRDSWCTLEVFEWLKKENRVDEEIMELMVSIMCGWVKKLIEDECN 165

Query: 185 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKD---- 244
              V DLL++MDCVGLKP FSM++KVI+LY EMG+KE A+ FVKEVL R+  F       
Sbjct: 166 AHQVFDLLIEMDCVGLKPGFSMMDKVIALYCEMGKKESAVLFVKEVLRRRDGFGYSVVGG 225

Query: 245 -DREGHKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEF 304
              EG KGGP GYLAWK MVDGDYR AV +V+ LR SGL PE YSYLIAMTA+VKELN  
Sbjct: 226 GGSEGRKGGPVGYLAWKFMVDGDYRKAVDMVMELRLSGLKPEAYSYLIAMTAIVKELNSL 285

Query: 305 AKALRKLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEG--SSSIHGVAH 364
            K LR+LK + R G VAE+D  +  L+E+YQ+E L+ G++L+ W +EEG  + SI GV H
Sbjct: 286 GKTLRELKRFARAGFVAEIDDHDRVLIEKYQSETLSRGLQLATWAVEEGQENDSIIGVVH 345

Query: 365 ERLLAMYICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEI 424
           ERLLAMYICAGRG EAE+QLW+MKL G+E ++DL+DIV+AICASQKE  A++RLLTR+E 
Sbjct: 346 ERLLAMYICAGRGPEAEKQLWKMKLAGREPEADLHDIVMAICASQKEVNAVSRLLTRVEF 405

Query: 425 ISPLLKKKSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPG 484
           +    KKK+LSWLLRGY+KGGHF +AAETLV M+D G  PEY+DRVAV+QG+ ++I+ P 
Sbjct: 406 MGSQRKKKTLSWLLRGYVKGGHFEEAAETLVSMIDSGLHPEYIDRVAVMQGMTRKIQRPR 465

Query: 485 SVETYFKLCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
            VE Y  LCK L DA L+GP LVY+++ K+KLW++KM+
Sbjct: 466 DVEAYMSLCKRLFDAGLVGPCLVYMYIDKYKLWIVKMM 503

[Close]

BLAST of MS014185.1 vs. TAIR 10
Match: AT1G10910.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 8.8e-08
Identity = 45/215 (20.93%), Postives = 87/215 (40.47%), Query Frame = 0


Query: 253 MMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALRKLKSYTRDGIVA 312
           ++ +G     +KL   ++  GL P+V +Y   +   +K  N + KA+  +     +GI  
Sbjct: 176 LVKNGKLDSCIKLFDQMKRDGLKPDVVTYNTLLAGCIKVKNGYPKAIELIGELPHNGI-- 235

Query: 313 ELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMYICAGRGLEAERQ 372
                                              +  V +  +LA+    GR  EAE  
Sbjct: 236 ----------------------------------QMDSVMYGTVLAICASNGRSEEAENF 295

Query: 373 LWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKKKSLSWLLRGYIK 432
           + +MK+ G   +   Y  +L   + + + +  + L+T ++ I  +  K  ++ LL+ YIK
Sbjct: 296 IQQMKVEGHSPNIYHYSSLLNSYSWKGDYKKADELMTEMKSIGLVPNKVMMTTLLKVYIK 354

Query: 433 GGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRK 468
           GG F  + E L ++   G+    +    ++ GL K
Sbjct: 356 GGLFDRSRELLSELESAGYAENEMPYCMLMDGLSK 354

The following BLAST results are available for this feature:

BLAST of MS014185.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148369.1	9.3e-294	99.61	pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Momordica ...	[more]
XP_038901728.1	2.2e-266	90.41	pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Benincasa ...	[more]
KAG7010495.1	2.0e-264	90.22	Pentatricopeptide repeat-containing protein, chloroplastic [Cucurbita argyrosper...	[more]
XP_023512972.1	5.9e-264	90.22	pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Cucurbita ...	[more]
XP_022944005.1	5.9e-264	90.22	pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Cucurbita ...	[more]

BLAST of MS014185.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WNN7	9.3e-172	64.85	Pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Arabidop...	[more]
Q0WVV0	1.2e-06	20.93	Pentatricopeptide repeat-containing protein At1g10910, chloroplastic OS=Arabidop...	[more]

BLAST of MS014185.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D3T2	4.5e-294	99.61	pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Momordic...	[more]
A0A6J1FYE9	2.8e-264	90.22	pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Cucurbit...	[more]
A0A6J1JH85	5.4e-263	89.63	pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Cucurbit...	[more]
A0A0A0KC35	3.5e-254	87.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G182120 PE=4 SV=1	[more]
A0A1S3CNE0	7.7e-254	87.28	pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Cucumis ...	[more]

BLAST of MS014185.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30100.1	6.6e-173	64.85	pentatricopeptide (PPR) repeat-containing protein	[more]
AT1G10910.1	8.8e-08	20.93	Pentatricopeptide repeat (PPR) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 252..489 e-value: 2.9E-10 score: 42.0
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 94..233 e-value: 4.0E-6 score: 28.5
None	No IPR available	PANTHER	PTHR47880	OS05G0353300 PROTEIN	coord: 4..511
IPR002885	Pentatricopeptide repeat	PROSITE	PS51375	PPR	coord: 419..453 score: 8.725252

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS014185	MS014185	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS014185.1-cds	MS014185.1-cds-scaffold5:1272153..1272923	CDS
MS014185.1-cds	MS014185.1-cds-scaffold5:1274019..1274780	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS014185.1	MS014185.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding