MS014185 (gene) Bitter gourd (TR) v1

Overview
NameMS014185
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionpentatricopeptide repeat-containing protein At2g30100, chloroplastic
Locationscaffold5: 1272153 .. 1274780 (-)
RNA-Seq ExpressionMS014185
SyntenyMS014185
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTGTGCCCAGGGCTTTACTCCGGTGACTCAATTTGGTTTCTCGTTTTCTTTATCTTCTGCACTGAAAACTCAGAGGCAAAGATTCTTCTCCACTCCCCAATTGTATTGCCACTCGCCGGTAAATTTTTGCTTTATGATTTCTTGCATTACCTGCAACCACCGGAATTCTACCTTCTCTGTTCTGAAAGCCGGTAAGTTTCGGGACCTGAGGTTGTTCAAATCGGTTGAGTTGGACCAGTTCATCACGAGTGACGACGAAGACGAAATGGGAGATGGATTTTTTGAGGCAATCGAGGAGTTGGAGCGCATGACGAGGGAACCATCGGATGTTCTCGAAGAAATGAATGACCGCCTTTCAGCGAGGGAATTCCAGCTTGTGCTTGTGTACTTCTCTCAAGAAGGGAGAGATTCATGGTGTGCTCTTGAGGTTTTTGAGTGGCTCCAGAAAGAAAATCGGGTCGACAAGGAGACCATGGAGCTGATGGTGTCTATTATGTGCAGTTGGATTAAAAAATTGGTTGAGGGAGACCATAACGTCGGAGATGTGGTTGACCTTCTCGTAGATATGGATTGTGTAGGTTTGAAACCTCATTTTAGCATGATAGAAAAAGTCATTTCCTTGTATTGGGAAATGGGCGAGAAGGAACAAGCTATTTCGTTCGTGAAAGAGGTCTTGGGACGCAAGATTGCTTTTATGAAGGACGATCGGGAGGGGCATAAAGGGGGACCAAGCGGTTATCTCGCATGGAAGATGATGGTAAGCCTTCTTCTAATCGCCATTTTCATATTTTAAGCCATTGACCTTGCTTAAAAGACAATATCAGTATTATTTTGCTGTGTCTATCTATTACATCAATAAATCGGAAATGAAATGTAAGTTTAGCACTTCGATCATTGTTTACTACACTTCAGGTGTCGTATTTTGAATCCTGAAATGCCTAATCTTAGAGATTCCTTACTCTGTCTATTTTACTCCTCTAGTACTCATTTAGGTTAGTATCTTAATCTTATTGGATTTTGATTATGCACTCTATATTCAGCATCGGGTTTTTGTTGTGATGGCTTTGGTCAAGGTGGTAAGGGATATTTTTTGGCGTTGTGTTTGTCTAATTGCAATTGAAGGGCTCCTGGAAGAACTGTGCAGGTCTATTCATCAGAGAGCTAGATGGGGCATTCAGGGTATTATGGTTTAACAGTAATTTTGAAATTTATGGTTAAGCTGCGTTCTATTGTGGTTAAGCATGTTTGAATCTATTGTTGTGTTTGAGTGTTGGACAAACTCAGTCAGTTATGTTGTTAGTGAAGTTTATTTCTGCAGAGGTAGGATTTGGAAAAACAATGAAATCTTGTTTAGGGTGTTTTTTTCCCTACCAGAACTATGGTGTCATTGGTTTCACTCATTGAAAACTTTAGGGCATGTCTTTGTTCATTGTAATTTTCTTTGGATCTTAGTTGTCGTTACTCTCCCATTAGTCAGAGTATTTGTATTTCTTGCCTCTTGTTCAATTTTTTTGGTCGTCGAATTGCCTCTCTCAATGTCATCATTTATGTTGACTGTTGTATTGGACTTCCATTTCATATGCATCACTACTACTATTCTCTTGTTTGGATTAGTGGCACACTCCTATGGTTTCATTCATATTCGAAAAACATTGAGCTGTATAAGACCGAGTTAATCCAATCTCGTGACTATATTAGCACACTGCTGTAATTCTTCTTCTCTTAACCAGCAATGGTTGTGGATTAGCTGCCCAAGGATTTTAAATTACTACTTTCAAATTGTTATGTTTGTTCAGCACAACCCCAAGTGAAAAATTCAATTGCTTATGATCTTGAAAATCAATTCTCTTAGGTTGATGGTGACTATAGGGGTGCAGTGAAATTGGTGCTGCATCTTAGAGAATCTGGATTAAATCCAGAGGTTTATAGCTACCTCATCGCCATGACTGCTGTGGTTAAAGAGCTGAATGAATTTGCAAAAGCTCTACGCAAACTCAAAAGTTACACAAGAGATGGAATAGTAGCCGAACTTGATAAAGACAATGTTGGACTTGTGGAGAGATATCAGACAGAGCTTCTAGCCGATGGAGTACGATTATCCAACTGGGTGCTTGAAGAGGGAAGCTCTTCAATTCATGGGGTGGCTCATGAGAGACTTCTTGCTATGTATATTTGCGCCGGGCGAGGACTTGAGGCAGAGAGACAACTTTGGGAAATGAAGCTTGTAGGTAAGGAGGCTGATAGTGATCTCTATGATATCGTGCTAGCCATCTGTGCTTCACAGAAGGAGACTAGAGCAATGAACCGGTTGCTTACCAGGATTGAGATTATAAGTCCCCTGCTGAAGAAGAAGAGCCTATCATGGCTGCTAAGGGGTTACATAAAAGGAGGGCATTTCAGTGATGCTGCAGAAACATTAGTAAAAATGGTTGATTTGGGTTTTCTCCCAGAATACTTGGACAGAGTAGCTGTGCTGCAAGGCCTAAGAAAACGGATTCGGGAACCTGGAAGTGTGGAAACTTACTTCAAGCTCTGCAAGTGTCTCTCAGATGCTAATCTGATTGGACCTAGTCTTGTATATTTGCACTTACAGAAACACAAGCTTTGGGTCATTAAAATGCTT

mRNA sequence

ATGATTTGTGCCCAGGGCTTTACTCCGGTGACTCAATTTGGTTTCTCGTTTTCTTTATCTTCTGCACTGAAAACTCAGAGGCAAAGATTCTTCTCCACTCCCCAATTGTATTGCCACTCGCCGGTAAATTTTTGCTTTATGATTTCTTGCATTACCTGCAACCACCGGAATTCTACCTTCTCTGTTCTGAAAGCCGGTAAGTTTCGGGACCTGAGGTTGTTCAAATCGGTTGAGTTGGACCAGTTCATCACGAGTGACGACGAAGACGAAATGGGAGATGGATTTTTTGAGGCAATCGAGGAGTTGGAGCGCATGACGAGGGAACCATCGGATGTTCTCGAAGAAATGAATGACCGCCTTTCAGCGAGGGAATTCCAGCTTGTGCTTGTGTACTTCTCTCAAGAAGGGAGAGATTCATGGTGTGCTCTTGAGGTTTTTGAGTGGCTCCAGAAAGAAAATCGGGTCGACAAGGAGACCATGGAGCTGATGGTGTCTATTATGTGCAGTTGGATTAAAAAATTGGTTGAGGGAGACCATAACGTCGGAGATGTGGTTGACCTTCTCGTAGATATGGATTGTGTAGGTTTGAAACCTCATTTTAGCATGATAGAAAAAGTCATTTCCTTGTATTGGGAAATGGGCGAGAAGGAACAAGCTATTTCGTTCGTGAAAGAGGTCTTGGGACGCAAGATTGCTTTTATGAAGGACGATCGGGAGGGGCATAAAGGGGGACCAAGCGGTTATCTCGCATGGAAGATGATGGTTGATGGTGACTATAGGGGTGCAGTGAAATTGGTGCTGCATCTTAGAGAATCTGGATTAAATCCAGAGGTTTATAGCTACCTCATCGCCATGACTGCTGTGGTTAAAGAGCTGAATGAATTTGCAAAAGCTCTACGCAAACTCAAAAGTTACACAAGAGATGGAATAGTAGCCGAACTTGATAAAGACAATGTTGGACTTGTGGAGAGATATCAGACAGAGCTTCTAGCCGATGGAGTACGATTATCCAACTGGGTGCTTGAAGAGGGAAGCTCTTCAATTCATGGGGTGGCTCATGAGAGACTTCTTGCTATGTATATTTGCGCCGGGCGAGGACTTGAGGCAGAGAGACAACTTTGGGAAATGAAGCTTGTAGGTAAGGAGGCTGATAGTGATCTCTATGATATCGTGCTAGCCATCTGTGCTTCACAGAAGGAGACTAGAGCAATGAACCGGTTGCTTACCAGGATTGAGATTATAAGTCCCCTGCTGAAGAAGAAGAGCCTATCATGGCTGCTAAGGGGTTACATAAAAGGAGGGCATTTCAGTGATGCTGCAGAAACATTAGTAAAAATGGTTGATTTGGGTTTTCTCCCAGAATACTTGGACAGAGTAGCTGTGCTGCAAGGCCTAAGAAAACGGATTCGGGAACCTGGAAGTGTGGAAACTTACTTCAAGCTCTGCAAGTGTCTCTCAGATGCTAATCTGATTGGACCTAGTCTTGTATATTTGCACTTACAGAAACACAAGCTTTGGGTCATTAAAATGCTT

Coding sequence (CDS)

ATGATTTGTGCCCAGGGCTTTACTCCGGTGACTCAATTTGGTTTCTCGTTTTCTTTATCTTCTGCACTGAAAACTCAGAGGCAAAGATTCTTCTCCACTCCCCAATTGTATTGCCACTCGCCGGTAAATTTTTGCTTTATGATTTCTTGCATTACCTGCAACCACCGGAATTCTACCTTCTCTGTTCTGAAAGCCGGTAAGTTTCGGGACCTGAGGTTGTTCAAATCGGTTGAGTTGGACCAGTTCATCACGAGTGACGACGAAGACGAAATGGGAGATGGATTTTTTGAGGCAATCGAGGAGTTGGAGCGCATGACGAGGGAACCATCGGATGTTCTCGAAGAAATGAATGACCGCCTTTCAGCGAGGGAATTCCAGCTTGTGCTTGTGTACTTCTCTCAAGAAGGGAGAGATTCATGGTGTGCTCTTGAGGTTTTTGAGTGGCTCCAGAAAGAAAATCGGGTCGACAAGGAGACCATGGAGCTGATGGTGTCTATTATGTGCAGTTGGATTAAAAAATTGGTTGAGGGAGACCATAACGTCGGAGATGTGGTTGACCTTCTCGTAGATATGGATTGTGTAGGTTTGAAACCTCATTTTAGCATGATAGAAAAAGTCATTTCCTTGTATTGGGAAATGGGCGAGAAGGAACAAGCTATTTCGTTCGTGAAAGAGGTCTTGGGACGCAAGATTGCTTTTATGAAGGACGATCGGGAGGGGCATAAAGGGGGACCAAGCGGTTATCTCGCATGGAAGATGATGGTTGATGGTGACTATAGGGGTGCAGTGAAATTGGTGCTGCATCTTAGAGAATCTGGATTAAATCCAGAGGTTTATAGCTACCTCATCGCCATGACTGCTGTGGTTAAAGAGCTGAATGAATTTGCAAAAGCTCTACGCAAACTCAAAAGTTACACAAGAGATGGAATAGTAGCCGAACTTGATAAAGACAATGTTGGACTTGTGGAGAGATATCAGACAGAGCTTCTAGCCGATGGAGTACGATTATCCAACTGGGTGCTTGAAGAGGGAAGCTCTTCAATTCATGGGGTGGCTCATGAGAGACTTCTTGCTATGTATATTTGCGCCGGGCGAGGACTTGAGGCAGAGAGACAACTTTGGGAAATGAAGCTTGTAGGTAAGGAGGCTGATAGTGATCTCTATGATATCGTGCTAGCCATCTGTGCTTCACAGAAGGAGACTAGAGCAATGAACCGGTTGCTTACCAGGATTGAGATTATAAGTCCCCTGCTGAAGAAGAAGAGCCTATCATGGCTGCTAAGGGGTTACATAAAAGGAGGGCATTTCAGTGATGCTGCAGAAACATTAGTAAAAATGGTTGATTTGGGTTTTCTCCCAGAATACTTGGACAGAGTAGCTGTGCTGCAAGGCCTAAGAAAACGGATTCGGGAACCTGGAAGTGTGGAAACTTACTTCAAGCTCTGCAAGTGTCTCTCAGATGCTAATCTGATTGGACCTAGTCTTGTATATTTGCACTTACAGAAACACAAGCTTTGGGTCATTAAAATGCTT

Protein sequence

MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTFSVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRLSAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHNVGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREGHKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALRKLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMYICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKKKSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFKLCKCLSDANLIGPSLVYLHLQKHKLWVIKML
Homology
BLAST of MS014185 vs. NCBI nr
Match: XP_022148369.1 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Momordica charantia])

HSP 1 Score: 1019.6 bits (2635), Expect = 9.3e-294
Identity = 509/511 (99.61%), Postives = 509/511 (99.61%), Query Frame = 0

Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF
Sbjct: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL
Sbjct: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSYTRDGIVAELDKDNVGLVE YQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY
Sbjct: 301 KLKSYTRDGIVAELDKDNVGLVEIYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK
Sbjct: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK
Sbjct: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGP LVYLHLQKHKLWVIKML
Sbjct: 481 LCKCLSDANLIGPGLVYLHLQKHKLWVIKML 511

BLAST of MS014185 vs. NCBI nr
Match: XP_038901728.1 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Benincasa hispida])

HSP 1 Score: 928.7 bits (2399), Expect = 2.2e-266
Identity = 462/511 (90.41%), Postives = 485/511 (94.91%), Query Frame = 0

Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           M+CAQGFTP+TQFGFSFSLSSALKT R   FSTPQLY   PV FCFM+S I+CN+++STF
Sbjct: 1   MVCAQGFTPLTQFGFSFSLSSALKTDRHG-FSTPQLYSPPPVKFCFMVSRISCNYQDSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +AGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL
Sbjct: 61  SVSRAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGQHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKE+AISFVKEVLGR +AFMKDD EG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEKAISFVKEVLGRNLAFMKDDWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VLHLRESGL PEVY YLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLHLRESGLKPEVYCYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDGIVAELDK+NV LVE+YQTELLADGVRLSNWVLEEGS SIHGV HERLLAMY
Sbjct: 301 KLKSYARDGIVAELDKNNVELVEKYQTELLADGVRLSNWVLEEGSFSIHGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+GLEAERQLWEMKLVGKEAD+DLYDIVLAICASQKET+AM RLLTRIEI SP+ KK
Sbjct: 361 ICAGQGLEAERQLWEMKLVGKEADADLYDIVLAICASQKETKAMKRLLTRIEITSPMRKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAAETLVKM+DLGFLPEYLDRVAVLQGLRK+IREP +V+TY  
Sbjct: 421 KSLTWLLRGYIKGGHFHDAAETLVKMIDLGFLPEYLDRVAVLQGLRKQIREPENVDTYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQKHKLWV+KML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVVKML 510

BLAST of MS014185 vs. NCBI nr
Match: KAG7010495.1 (Pentatricopeptide repeat-containing protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 922.2 bits (2382), Expect = 2.0e-264
Identity = 461/511 (90.22%), Postives = 485/511 (94.91%), Query Frame = 0

Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGF+P+TQFGFSFSLSS LK++R   FS PQL   SPVNFCFM+S ITCNH+NSTF
Sbjct: 1   MICAQGFSPLTQFGFSFSLSSGLKSERLG-FSAPQLCSRSPVNFCFMVSRITCNHQNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +AGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTR+PSDVLEEMNDRL
Sbjct: 61  SVSRAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTRDPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGQHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYW+MGEKE+AISFVKEVLGRK+ FMKD+ EG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWDMGEKEKAISFVKEVLGRKLDFMKDNWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VL+LRESGL PEVY YLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLNLRESGLKPEVYCYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDG+VAELDKDNV LV+RYQ+ELLADGVRLSNWVL+EG SS HGV HERLLAMY
Sbjct: 301 KLKSYARDGMVAELDKDNVELVKRYQSELLADGVRLSNWVLDEGGSSSHGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+GLEAERQLWEMKLVGKEAD+DLYDIVLAICASQKETRAMNRLLTRIEI SP LKK
Sbjct: 361 ICAGQGLEAERQLWEMKLVGKEADADLYDIVLAICASQKETRAMNRLLTRIEITSPRLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAAETLVKMV+LGFLPEYLDRVAVLQGLRKRIREP +VETY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAETLVKMVNLGFLPEYLDRVAVLQGLRKRIREPENVETYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQK+KLWVIKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKYKLWVIKML 510

BLAST of MS014185 vs. NCBI nr
Match: XP_023512972.1 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 920.6 bits (2378), Expect = 5.9e-264
Identity = 461/511 (90.22%), Postives = 484/511 (94.72%), Query Frame = 0

Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTP+TQFGFSFSLSS LK++R   FS PQL   SPVNFCFM+S ITCNH+NSTF
Sbjct: 1   MICAQGFTPLTQFGFSFSLSSGLKSERLG-FSAPQLCSRSPVNFCFMVSRITCNHQNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +AGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTR+PSDVLEEMNDRL
Sbjct: 61  SVSRAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTRDPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGQHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYW+MGEKE+AISFVKEVLGRK+ FMKD+ EG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWDMGEKEKAISFVKEVLGRKLDFMKDNWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VL+LRESGL PEVY YLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLNLRESGLKPEVYCYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDG+VAELDKDNV LV+RYQ+ELLADGVRLSNWVL+EG SS H V HERLLAMY
Sbjct: 301 KLKSYARDGMVAELDKDNVELVKRYQSELLADGVRLSNWVLDEGGSSSHRVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+GLEAERQLWEMKLVGKEAD+DLYDIVLAICASQKETRAMNRLLTRIEI SP LKK
Sbjct: 361 ICAGQGLEAERQLWEMKLVGKEADADLYDIVLAICASQKETRAMNRLLTRIEITSPRLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAAETLVKMV+LGFLPEYLDRVAVLQGLRKRIREP +VETY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAETLVKMVNLGFLPEYLDRVAVLQGLRKRIREPENVETYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQK+KLWVIKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKYKLWVIKML 510

BLAST of MS014185 vs. NCBI nr
Match: XP_022944005.1 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Cucurbita moschata])

HSP 1 Score: 920.6 bits (2378), Expect = 5.9e-264
Identity = 461/511 (90.22%), Postives = 484/511 (94.72%), Query Frame = 0

Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTP+TQFGFSFSLSS LK++R   FS PQL   SPVNFCFM+S ITCNH+NSTF
Sbjct: 1   MICAQGFTPLTQFGFSFSLSSGLKSERLG-FSAPQLCSRSPVNFCFMVSRITCNHQNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +AGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTR+PSDVLEEMNDRL
Sbjct: 61  SVSRAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTRDPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGQHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           V DVVDLLVDMDCVGLKPHFSMIEKVISLYW+MGEKE+AISFVKEVLGRK+ FMKD+ EG
Sbjct: 181 VRDVVDLLVDMDCVGLKPHFSMIEKVISLYWDMGEKEKAISFVKEVLGRKLDFMKDNWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VL+LRESGL PEVY YLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLNLRESGLKPEVYCYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDG+VAELDKDNV LV+RYQ+ELLADGVRLSNWVL+EG SS HGV HERLLAMY
Sbjct: 301 KLKSYARDGMVAELDKDNVELVKRYQSELLADGVRLSNWVLDEGGSSSHGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+GLEAERQLWEMKLVGKEAD+DLYDIVLAICASQKETRAMNRLLTRIEI SP LKK
Sbjct: 361 ICAGQGLEAERQLWEMKLVGKEADADLYDIVLAICASQKETRAMNRLLTRIEITSPRLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAAETLVKMV+LGFLPEYLDRVAVLQGLRKRIREP +VETY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAETLVKMVNLGFLPEYLDRVAVLQGLRKRIREPENVETYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQK+KLWVIKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKYKLWVIKML 510

BLAST of MS014185 vs. ExPASy Swiss-Prot
Match: Q0WNN7 (Pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g30100 PE=2 SV=2)

HSP 1 Score: 604.7 bits (1558), Expect = 9.3e-172
Identity = 297/458 (64.85%), Postives = 367/458 (80.13%), Query Frame = 0

Query: 65  AGKFRDLRLFKSVELDQFITSDDE----DEMGDGFFEAIEELERMTREPSDVLEEMNDRL 124
           AGKFR++ L +SVELDQFITS++E    +E+G+GFFEAIEELERMTREPSD+LEEMN RL
Sbjct: 46  AGKFREMGLSRSVELDQFITSEEEEGEAEEIGEGFFEAIEELERMTREPSDILEEMNHRL 105

Query: 125 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 184
           S+RE QL+LVYF+QEGRDSWC LEVFEWL+KENRVD+E MELMVSIMC W+KKL+E + N
Sbjct: 106 SSRELQLMLVYFAQEGRDSWCTLEVFEWLKKENRVDEEIMELMVSIMCGWVKKLIEDECN 165

Query: 185 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKD---- 244
              V DLL++MDCVGLKP FSM++KVI+LY EMG+KE A+ FVKEVL R+  F       
Sbjct: 166 AHQVFDLLIEMDCVGLKPGFSMMDKVIALYCEMGKKESAVLFVKEVLRRRDGFGYSVVGG 225

Query: 245 -DREGHKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEF 304
              EG KGGP GYLAWK MVDGDYR AV +V+ LR SGL PE YSYLIAMTA+VKELN  
Sbjct: 226 GGSEGRKGGPVGYLAWKFMVDGDYRKAVDMVMELRLSGLKPEAYSYLIAMTAIVKELNSL 285

Query: 305 AKALRKLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEG--SSSIHGVAH 364
            K LR+LK + R G VAE+D  +  L+E+YQ+E L+ G++L+ W +EEG  + SI GV H
Sbjct: 286 GKTLRELKRFARAGFVAEIDDHDRVLIEKYQSETLSRGLQLATWAVEEGQENDSIIGVVH 345

Query: 365 ERLLAMYICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEI 424
           ERLLAMYICAGRG EAE+QLW+MKL G+E ++DL+DIV+AICASQKE  A++RLLTR+E 
Sbjct: 346 ERLLAMYICAGRGPEAEKQLWKMKLAGREPEADLHDIVMAICASQKEVNAVSRLLTRVEF 405

Query: 425 ISPLLKKKSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPG 484
           +    KKK+LSWLLRGY+KGGHF +AAETLV M+D G  PEY+DRVAV+QG+ ++I+ P 
Sbjct: 406 MGSQRKKKTLSWLLRGYVKGGHFEEAAETLVSMIDSGLHPEYIDRVAVMQGMTRKIQRPR 465

Query: 485 SVETYFKLCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
            VE Y  LCK L DA L+GP LVY+++ K+KLW++KM+
Sbjct: 466 DVEAYMSLCKRLFDAGLVGPCLVYMYIDKYKLWIVKMM 503

BLAST of MS014185 vs. ExPASy Swiss-Prot
Match: Q0WVV0 (Pentatricopeptide repeat-containing protein At1g10910, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g10910 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.2e-06
Identity = 45/215 (20.93%), Postives = 87/215 (40.47%), Query Frame = 0

Query: 253 MMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALRKLKSYTRDGIVA 312
           ++ +G     +KL   ++  GL P+V +Y   +   +K  N + KA+  +     +GI  
Sbjct: 176 LVKNGKLDSCIKLFDQMKRDGLKPDVVTYNTLLAGCIKVKNGYPKAIELIGELPHNGI-- 235

Query: 313 ELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMYICAGRGLEAERQ 372
                                              +  V +  +LA+    GR  EAE  
Sbjct: 236 ----------------------------------QMDSVMYGTVLAICASNGRSEEAENF 295

Query: 373 LWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKKKSLSWLLRGYIK 432
           + +MK+ G   +   Y  +L   + + + +  + L+T ++ I  +  K  ++ LL+ YIK
Sbjct: 296 IQQMKVEGHSPNIYHYSSLLNSYSWKGDYKKADELMTEMKSIGLVPNKVMMTTLLKVYIK 354

Query: 433 GGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRK 468
           GG F  + E L ++   G+    +    ++ GL K
Sbjct: 356 GGLFDRSRELLSELESAGYAENEMPYCMLMDGLSK 354

BLAST of MS014185 vs. ExPASy TrEMBL
Match: A0A6J1D3T2 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111017036 PE=4 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 4.5e-294
Identity = 509/511 (99.61%), Postives = 509/511 (99.61%), Query Frame = 0

Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF
Sbjct: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL
Sbjct: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSYTRDGIVAELDKDNVGLVE YQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY
Sbjct: 301 KLKSYTRDGIVAELDKDNVGLVEIYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK
Sbjct: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK
Sbjct: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGP LVYLHLQKHKLWVIKML
Sbjct: 481 LCKCLSDANLIGPGLVYLHLQKHKLWVIKML 511

BLAST of MS014185 vs. ExPASy TrEMBL
Match: A0A6J1FYE9 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448564 PE=4 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 2.8e-264
Identity = 461/511 (90.22%), Postives = 484/511 (94.72%), Query Frame = 0

Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTP+TQFGFSFSLSS LK++R   FS PQL   SPVNFCFM+S ITCNH+NSTF
Sbjct: 1   MICAQGFTPLTQFGFSFSLSSGLKSERLG-FSAPQLCSRSPVNFCFMVSRITCNHQNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +AGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTR+PSDVLEEMNDRL
Sbjct: 61  SVSRAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTRDPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGQHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           V DVVDLLVDMDCVGLKPHFSMIEKVISLYW+MGEKE+AISFVKEVLGRK+ FMKD+ EG
Sbjct: 181 VRDVVDLLVDMDCVGLKPHFSMIEKVISLYWDMGEKEKAISFVKEVLGRKLDFMKDNWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VL+LRESGL PEVY YLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLNLRESGLKPEVYCYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDG+VAELDKDNV LV+RYQ+ELLADGVRLSNWVL+EG SS HGV HERLLAMY
Sbjct: 301 KLKSYARDGMVAELDKDNVELVKRYQSELLADGVRLSNWVLDEGGSSSHGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+GLEAERQLWEMKLVGKEAD+DLYDIVLAICASQKETRAMNRLLTRIEI SP LKK
Sbjct: 361 ICAGQGLEAERQLWEMKLVGKEADADLYDIVLAICASQKETRAMNRLLTRIEITSPRLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAAETLVKMV+LGFLPEYLDRVAVLQGLRKRIREP +VETY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAETLVKMVNLGFLPEYLDRVAVLQGLRKRIREPENVETYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQK+KLWVIKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKYKLWVIKML 510

BLAST of MS014185 vs. ExPASy TrEMBL
Match: A0A6J1JH85 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484464 PE=4 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.4e-263
Identity = 458/511 (89.63%), Postives = 485/511 (94.91%), Query Frame = 0

Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICA GFTP+T+FGFSFSLSS LK++R   FS PQL   SPVNFCF++S ITCNH+NSTF
Sbjct: 1   MICAPGFTPLTKFGFSFSLSSGLKSKRLG-FSAPQLCSRSPVNFCFIVSRITCNHQNSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +AGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTR+PSDVLEEMNDRL
Sbjct: 61  SVSRAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTRDPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGQHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYW+MGEKE+AISFVKEVLGRK+ FMKD+ EG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWDMGEKEKAISFVKEVLGRKLDFMKDNWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VL+LRESGL PEVY +LIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLNLRESGLKPEVYCFLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDG+VAELDKDNV LV+RYQ+ELLADGVRLSNWVL+EGSSS HGV HERLLAMY
Sbjct: 301 KLKSYARDGMVAELDKDNVELVKRYQSELLADGVRLSNWVLDEGSSSSHGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+GLEAERQLWEMKLVGKEAD+DLYDIVLAICASQKETRAMNRLL+RIEI SP LKK
Sbjct: 361 ICAGQGLEAERQLWEMKLVGKEADADLYDIVLAICASQKETRAMNRLLSRIEITSPRLKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAAETLVKMV+LGFLPEYLDRVAVLQGLRKRIREP +VETY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAETLVKMVNLGFLPEYLDRVAVLQGLRKRIREPENVETYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQK+KLWVIKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKYKLWVIKML 510

BLAST of MS014185 vs. ExPASy TrEMBL
Match: A0A0A0KC35 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G182120 PE=4 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.5e-254
Identity = 446/511 (87.28%), Postives = 473/511 (92.56%), Query Frame = 0

Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTP+TQFGFSFSLSS L++QR   FSTP+LY         M+S I+CN+++STF
Sbjct: 1   MICAQGFTPLTQFGFSFSLSSPLESQRCG-FSTPRLY---------MVSPISCNYQDSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +A KFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL
Sbjct: 61  SVSRAAKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SARE QLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEG HN
Sbjct: 121 SAREIQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGRHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKE+A+ FVKEVLGR +AFMKDD EG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEKAVFFVKEVLGRNLAFMKDDWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VLHLRESGL PEVYSYLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLHLRESGLRPEVYSYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLK Y RDG VAELDK+NV LV +YQTELLADGV+LSNWVLEEGSSSI GV HERLLAMY
Sbjct: 301 KLKGYARDGFVAELDKNNVELVAKYQTELLADGVQLSNWVLEEGSSSIRGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+G+EAERQLWEMKLVGKEAD+DLYDIVLAICASQKET+AM RLLTRIEI SP++KK
Sbjct: 361 ICAGQGVEAERQLWEMKLVGKEADADLYDIVLAICASQKETKAMKRLLTRIEITSPMIKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAA TLVKM++LGFLPEYLDRVAVLQGLRK IREP SV TY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAGTLVKMINLGFLPEYLDRVAVLQGLRKEIREPESVHTYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQKHKLW+IKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKHKLWIIKML 501

BLAST of MS014185 vs. ExPASy TrEMBL
Match: A0A1S3CNE0 (pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502924 PE=4 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 7.7e-254
Identity = 446/511 (87.28%), Postives = 472/511 (92.37%), Query Frame = 0

Query: 1   MICAQGFTPVTQFGFSFSLSSALKTQRQRFFSTPQLYCHSPVNFCFMISCITCNHRNSTF 60
           MICAQGFTP+TQFGFSFSLSS L+TQR   FSTP+LY         M+S I+CN+++STF
Sbjct: 1   MICAQGFTPLTQFGFSFSLSSPLETQRYG-FSTPRLY---------MVSPISCNYQDSTF 60

Query: 61  SVLKAGKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120
           SV +A KFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL
Sbjct: 61  SVSRAAKFRDLRLFKSVELDQFITSDDEDEMGDGFFEAIEELERMTREPSDVLEEMNDRL 120

Query: 121 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 180
           SARE QLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWI KLVEG HN
Sbjct: 121 SAREIQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWINKLVEGRHN 180

Query: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKDDREG 240
           VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKE+AI FVKEVLGR +AFMKDD EG
Sbjct: 181 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEKAIFFVKEVLGRNLAFMKDDWEG 240

Query: 241 HKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALR 300
           HKGGPSGYLAWKMMVDGDYRGAVK+VLHLRESGL PEVYSYLIAMTAVVKELNEFAKALR
Sbjct: 241 HKGGPSGYLAWKMMVDGDYRGAVKMVLHLRESGLRPEVYSYLIAMTAVVKELNEFAKALR 300

Query: 301 KLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMY 360
           KLKSY RDG VAELDK+NV LV +YQTELLADGVRLSNWVLEEGSSSIHGV HERLLAMY
Sbjct: 301 KLKSYARDGYVAELDKNNVELVAKYQTELLADGVRLSNWVLEEGSSSIHGVVHERLLAMY 360

Query: 361 ICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKK 420
           ICAG+G+EAERQLWEMKL+GKEAD+DLYDIVLAICASQKE +AM RLLTRIEI SP++KK
Sbjct: 361 ICAGQGVEAERQLWEMKLLGKEADADLYDIVLAICASQKEIKAMKRLLTRIEITSPMIKK 420

Query: 421 KSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPGSVETYFK 480
           KSL+WLLRGYIKGGHF DAA T+VKM++LGFLPEYLDRVAVLQGLRK IREP  V TY  
Sbjct: 421 KSLTWLLRGYIKGGHFRDAAGTVVKMINLGFLPEYLDRVAVLQGLRKGIREPEIVHTYLD 480

Query: 481 LCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
           LCKCLSDANLIGPSLVYLHLQKHKLW+IKML
Sbjct: 481 LCKCLSDANLIGPSLVYLHLQKHKLWIIKML 501

BLAST of MS014185 vs. TAIR 10
Match: AT2G30100.1 (pentatricopeptide (PPR) repeat-containing protein )

HSP 1 Score: 604.7 bits (1558), Expect = 6.6e-173
Identity = 297/458 (64.85%), Postives = 367/458 (80.13%), Query Frame = 0

Query: 65  AGKFRDLRLFKSVELDQFITSDDE----DEMGDGFFEAIEELERMTREPSDVLEEMNDRL 124
           AGKFR++ L +SVELDQFITS++E    +E+G+GFFEAIEELERMTREPSD+LEEMN RL
Sbjct: 46  AGKFREMGLSRSVELDQFITSEEEEGEAEEIGEGFFEAIEELERMTREPSDILEEMNHRL 105

Query: 125 SAREFQLVLVYFSQEGRDSWCALEVFEWLQKENRVDKETMELMVSIMCSWIKKLVEGDHN 184
           S+RE QL+LVYF+QEGRDSWC LEVFEWL+KENRVD+E MELMVSIMC W+KKL+E + N
Sbjct: 106 SSRELQLMLVYFAQEGRDSWCTLEVFEWLKKENRVDEEIMELMVSIMCGWVKKLIEDECN 165

Query: 185 VGDVVDLLVDMDCVGLKPHFSMIEKVISLYWEMGEKEQAISFVKEVLGRKIAFMKD---- 244
              V DLL++MDCVGLKP FSM++KVI+LY EMG+KE A+ FVKEVL R+  F       
Sbjct: 166 AHQVFDLLIEMDCVGLKPGFSMMDKVIALYCEMGKKESAVLFVKEVLRRRDGFGYSVVGG 225

Query: 245 -DREGHKGGPSGYLAWKMMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEF 304
              EG KGGP GYLAWK MVDGDYR AV +V+ LR SGL PE YSYLIAMTA+VKELN  
Sbjct: 226 GGSEGRKGGPVGYLAWKFMVDGDYRKAVDMVMELRLSGLKPEAYSYLIAMTAIVKELNSL 285

Query: 305 AKALRKLKSYTRDGIVAELDKDNVGLVERYQTELLADGVRLSNWVLEEG--SSSIHGVAH 364
            K LR+LK + R G VAE+D  +  L+E+YQ+E L+ G++L+ W +EEG  + SI GV H
Sbjct: 286 GKTLRELKRFARAGFVAEIDDHDRVLIEKYQSETLSRGLQLATWAVEEGQENDSIIGVVH 345

Query: 365 ERLLAMYICAGRGLEAERQLWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEI 424
           ERLLAMYICAGRG EAE+QLW+MKL G+E ++DL+DIV+AICASQKE  A++RLLTR+E 
Sbjct: 346 ERLLAMYICAGRGPEAEKQLWKMKLAGREPEADLHDIVMAICASQKEVNAVSRLLTRVEF 405

Query: 425 ISPLLKKKSLSWLLRGYIKGGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRKRIREPG 484
           +    KKK+LSWLLRGY+KGGHF +AAETLV M+D G  PEY+DRVAV+QG+ ++I+ P 
Sbjct: 406 MGSQRKKKTLSWLLRGYVKGGHFEEAAETLVSMIDSGLHPEYIDRVAVMQGMTRKIQRPR 465

Query: 485 SVETYFKLCKCLSDANLIGPSLVYLHLQKHKLWVIKML 512
            VE Y  LCK L DA L+GP LVY+++ K+KLW++KM+
Sbjct: 466 DVEAYMSLCKRLFDAGLVGPCLVYMYIDKYKLWIVKMM 503

BLAST of MS014185 vs. TAIR 10
Match: AT1G10910.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 8.8e-08
Identity = 45/215 (20.93%), Postives = 87/215 (40.47%), Query Frame = 0

Query: 253 MMVDGDYRGAVKLVLHLRESGLNPEVYSYLIAMTAVVKELNEFAKALRKLKSYTRDGIVA 312
           ++ +G     +KL   ++  GL P+V +Y   +   +K  N + KA+  +     +GI  
Sbjct: 176 LVKNGKLDSCIKLFDQMKRDGLKPDVVTYNTLLAGCIKVKNGYPKAIELIGELPHNGI-- 235

Query: 313 ELDKDNVGLVERYQTELLADGVRLSNWVLEEGSSSIHGVAHERLLAMYICAGRGLEAERQ 372
                                              +  V +  +LA+    GR  EAE  
Sbjct: 236 ----------------------------------QMDSVMYGTVLAICASNGRSEEAENF 295

Query: 373 LWEMKLVGKEADSDLYDIVLAICASQKETRAMNRLLTRIEIISPLLKKKSLSWLLRGYIK 432
           + +MK+ G   +   Y  +L   + + + +  + L+T ++ I  +  K  ++ LL+ YIK
Sbjct: 296 IQQMKVEGHSPNIYHYSSLLNSYSWKGDYKKADELMTEMKSIGLVPNKVMMTTLLKVYIK 354

Query: 433 GGHFSDAAETLVKMVDLGFLPEYLDRVAVLQGLRK 468
           GG F  + E L ++   G+    +    ++ GL K
Sbjct: 356 GGLFDRSRELLSELESAGYAENEMPYCMLMDGLSK 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148369.19.3e-29499.61pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Momordica ... [more]
XP_038901728.12.2e-26690.41pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Benincasa ... [more]
KAG7010495.12.0e-26490.22Pentatricopeptide repeat-containing protein, chloroplastic [Cucurbita argyrosper... [more]
XP_023512972.15.9e-26490.22pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Cucurbita ... [more]
XP_022944005.15.9e-26490.22pentatricopeptide repeat-containing protein At2g30100, chloroplastic [Cucurbita ... [more]
Match NameE-valueIdentityDescription
Q0WNN79.3e-17264.85Pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Arabidop... [more]
Q0WVV01.2e-0620.93Pentatricopeptide repeat-containing protein At1g10910, chloroplastic OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1D3T24.5e-29499.61pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Momordic... [more]
A0A6J1FYE92.8e-26490.22pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Cucurbit... [more]
A0A6J1JH855.4e-26389.63pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Cucurbit... [more]
A0A0A0KC353.5e-25487.28Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G182120 PE=4 SV=1[more]
A0A1S3CNE07.7e-25487.28pentatricopeptide repeat-containing protein At2g30100, chloroplastic OS=Cucumis ... [more]
Match NameE-valueIdentityDescription
AT2G30100.16.6e-17364.85pentatricopeptide (PPR) repeat-containing protein [more]
AT1G10910.18.8e-0820.93Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 252..489
e-value: 2.9E-10
score: 42.0
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 94..233
e-value: 4.0E-6
score: 28.5
NoneNo IPR availablePANTHERPTHR47880OS05G0353300 PROTEINcoord: 4..511
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 419..453
score: 8.725252

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS014185.1MS014185.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding