MS009218.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS009218.1 vs. NCBI nr
Match: XP_022133537.1 (UPF0496 protein 3-like [Momordica charantia])

HSP 1 Score: 706.4 bits (1822), Expect = 1.3e-199
Identity = 371/372 (99.73%), Postives = 371/372 (99.73%), Query Frame = 0

Query: 1   MWPKIRDLKIRNSTHISSSIFACSAAQLLNFSQPVLISAFADAKQQIRNKPQKSFNVNDE 60
           MWPKIRDLKIRNSTHISSSIFACSAAQLLNFSQPVLISAFADAKQQIRNKPQKSFNVNDE
Sbjct: 1   MWPKIRDLKIRNSTHISSSIFACSAAQLLNFSQPVLISAFADAKQQIRNKPQKSFNVNDE 60

Query: 61  YICALRTKSYVEFFIKAQSVIEESSPPSTSSSTGRRRRRKLSETILLEPSQEAIPSILES 120
           YICALRTKSYVEFFIKAQSVIEESSPPSTSSSTGRRRRRKLS TILLEPSQEAIPSILES
Sbjct: 61  YICALRTKSYVEFFIKAQSVIEESSPPSTSSSTGRRRRRKLSGTILLEPSQEAIPSILES 120

Query: 121 PFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSLDSIENCSSPETI 180
           PFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSLDSIENCSSPETI
Sbjct: 121 PFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSLDSIENCSSPETI 180

Query: 181 ETIASEFLALREPFSDPDKHDFALIHDDYEAVSRRLNCTRKKVARKIKSIEIIETISCGL 240
           ETIASEFLALREPFSDPDKHDFALIHDDYEAVSRRLNCTRKKVARKIKSIEIIETISCGL
Sbjct: 181 ETIASEFLALREPFSDPDKHDFALIHDDYEAVSRRLNCTRKKVARKIKSIEIIETISCGL 240

Query: 241 VAITARTLTELFRFPPESFRRKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSR 300
           VAITARTLTELFRFPPESFRRKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSR
Sbjct: 241 VAITARTLTELFRFPPESFRRKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSR 300

Query: 301 LVARLDDAMDHGKAMARLFVERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVT 360
           LVARLDDAMDHGKAMARLFVERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVT
Sbjct: 301 LVARLDDAMDHGKAMARLFVERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVT 360

Query: 361 INRARGLVINQI 373
           INRARGLVINQI
Sbjct: 361 INRARGLVINQI 372

BLAST of MS009218.1 vs. NCBI nr
Match: XP_038884256.1 (UPF0496 protein At3g49070-like [Benincasa hispida])

HSP 1 Score: 459.1 bits (1180), Expect = 3.5e-125
Identity = 251/385 (65.19%), Postives = 300/385 (77.92%), Query Frame = 0

Query: 1   MWPKIRDLKIRNSTHISSSIFACSAAQLLNFS--QPVLISAFADAKQQIRNKPQKSFNVN 60
           MW K R  +IRNST +S SI AC  A L NFS  QPV IS FAD +Q+I  KPQKSFNVN
Sbjct: 1   MWTKFRHSEIRNSTKLSQSIIAC-FAHLCNFSQTQPVFISIFADEEQEIGKKPQKSFNVN 60

Query: 61  DEYICALRTKSYVEFFIKAQSVIEESSPPSTSSSTGRRRRRKLSETILLEPSQ-EAIPSI 120
           +EY+C LRTKS+ EFF KA+ +++ES P  TSSS    R RKLSETILL+P + EA+PSI
Sbjct: 61  EEYLCTLRTKSFSEFFTKAEFIVDESPPSITSSSAAADRCRKLSETILLQPVRLEAVPSI 120

Query: 121 LESPFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSLDSIENCSSP 180
           LES FLLMLP+LK L IDY NVSA+AS  CTRLL +V+ TRS SR IQ+SLDSIE C S 
Sbjct: 121 LESSFLLMLPELKGLFIDYLNVSAKASNLCTRLLTNVKSTRSKSRCIQQSLDSIEKCFSS 180

Query: 181 ETIETIASEFLALREPFSDPDKHDFALIHDDYEAVSRRLNCTRKKVARKIKSIEIIETIS 240
           ETIE+IAS+ L+LR PFSD DK DF LIH DY A+SRRLNCTRKKV RKI++I+II+ I+
Sbjct: 181 ETIESIASDLLSLRAPFSDLDKRDFTLIHADYTAISRRLNCTRKKVERKIRTIKIIDGIT 240

Query: 241 CGLVAITARTLTE----------LFRFPPESFRRKLLRYQMIRNGGLGEVGEQLEAAAKG 300
           CGL +IT RTLT+          +F +  +S  RKL+R++M+RNGGL +VGE++EAAAKG
Sbjct: 241 CGLNSITTRTLTDSVMAKDPGPVIFGYRLKSLGRKLVRHEMLRNGGLEKVGEKVEAAAKG 300

Query: 301 SYILNREFDTTSRLVARLDDAMDHGKAMARLFVERKEDKFAVQVAMDELKKSNLRLRNQV 360
           SYI+ RE DTTSRLV RL DA+DHGKAM RLF  RKEDKFAV VAMDE+KK+N+ +R QV
Sbjct: 301 SYIMKRELDTTSRLVVRLGDAVDHGKAMLRLFGGRKEDKFAVSVAMDEVKKNNVSMRKQV 360

Query: 361 EEVEEHLYLCIVTINRARGLVINQI 373
           E+VEEHLYLCIVTINR+R  VI Q+
Sbjct: 361 EDVEEHLYLCIVTINRSRASVIKQL 384

BLAST of MS009218.1 vs. NCBI nr
Match: KAG6602186.1 (UPF0496 protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 438.3 bits (1126), Expect = 6.4e-119
Identity = 243/352 (69.03%), Postives = 280/352 (79.55%), Query Frame = 0

Query: 32  SQPVLISAFADAKQQIRNKPQKSFNVNDEYICALRTKSYVEFFIKAQSVIEESSPPSTSS 91
           S+ + IS FAD K QIRNKPQKSFNVN+EY+CALR++S+VEFF KAQ +++E SPPSTSS
Sbjct: 8   SRFIWISVFADVKHQIRNKPQKSFNVNEEYLCALRSRSFVEFFAKAQLIVQE-SPPSTSS 67

Query: 92  STGRRRRRKLSETILLEPSQ-EAIPSILESPFLLMLPDLKSLLIDYFNVSAEASKFCTRL 151
           S      RK SETILL+  Q EA PSILESP L+MLP+L+ LLID+FNVSAEAS  C RL
Sbjct: 68  SAD-LYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASNLCHRL 127

Query: 152 LEDVELTRSNSRSIQKSLDSIENCSSPETIETIASEFLALREPFSDPDKHDFALIHDDYE 211
           L +++L RSNSR +QK LDSIE CSSP+ IETI S+ LA R P SD DK DFA IHDDY 
Sbjct: 128 LANLKLARSNSRFVQKLLDSIEKCSSPKEIETITSDLLAQRAPLSDLDKRDFARIHDDYA 187

Query: 212 AVSRRLNCTRKKVARKIKSIEIIETISCGLVAITARTLTELFR----------FPPESFR 271
           AVSR LN TRKKVARKI+SI+II+  +CGLVAIT+R LT L            F  +S R
Sbjct: 188 AVSRSLNITRKKVARKIRSIKIIDRFTCGLVAITSRGLTALVMAADQGPVLRYFRLKSIR 247

Query: 272 RKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGKAMARLFV 331
           RKLLR+QM+ NGGL  VGEQLEAAAKGSYILNREFDTTSRLV RL DA+DHGKAM RLFV
Sbjct: 248 RKLLRHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFV 307

Query: 332 ERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVINQI 373
           ERKEDKFAV VAMDE+K+SN+ +R QVE+VEEHLYLC VTINR+R  VINQ+
Sbjct: 308 ERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCFVTINRSRASVINQM 357

BLAST of MS009218.1 vs. NCBI nr
Match: XP_022990396.1 (UPF0496 protein 3-like [Cucurbita maxima])

HSP 1 Score: 438.0 bits (1125), Expect = 8.4e-119
Identity = 245/352 (69.60%), Postives = 281/352 (79.83%), Query Frame = 0

Query: 32  SQPVLISAFADAKQQIRNKPQKSFNVNDEYICALRTKSYVEFFIKAQSVIEESSPPSTSS 91
           S+ + IS FAD K QIRNKPQKSFNVN+EY+CALR++S+VEFF KAQ +++E SPPST S
Sbjct: 8   SRFIWISVFADVKHQIRNKPQKSFNVNEEYLCALRSRSFVEFFAKAQLIVQE-SPPST-S 67

Query: 92  STGRRRRRKLSETILLEPSQ-EAIPSILESPFLLMLPDLKSLLIDYFNVSAEASKFCTRL 151
           STG    RK SETILL+  Q EA PSILESP L+MLP+L+ LLID+FNVSAEAS  C RL
Sbjct: 68  STG-HHCRKFSETILLQIDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASNLCHRL 127

Query: 152 LEDVELTRSNSRSIQKSLDSIENCSSPETIETIASEFLALREPFSDPDKHDFALIHDDYE 211
           L +++L RSNSR +QK LDSIE CSSP+ IETI S+ LA R P SD DK DFA IHDDY 
Sbjct: 128 LANLKLARSNSRFVQKLLDSIEKCSSPKEIETITSDLLAQRAPLSDLDKRDFARIHDDYA 187

Query: 212 AVSRRLNCTRKKVARKIKSIEIIETISCGLVAITARTLTELFR----------FPPESFR 271
           AVSR LN TRKKVARKI+SI+II   +CGLVAIT+R LT L            F  +S R
Sbjct: 188 AVSRSLNITRKKVARKIRSIKIINKCTCGLVAITSRGLTALVMAADQGPVLRYFRLKSIR 247

Query: 272 RKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGKAMARLFV 331
           RKLLR+QM+ NGGL  VGEQLEAAAKGSYILNREFDTTSRLV RL DA+DHGKAM RLFV
Sbjct: 248 RKLLRHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFV 307

Query: 332 ERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVINQI 373
           ERKEDKFAV VAMDE+K+SN+ +R QVE+VEEHLYLCIVTINR+R  VINQ+
Sbjct: 308 ERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 356

BLAST of MS009218.1 vs. NCBI nr
Match: KAG7032870.1 (UPF0496 protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 438.0 bits (1125), Expect = 8.4e-119
Identity = 243/352 (69.03%), Postives = 280/352 (79.55%), Query Frame = 0

Query: 32  SQPVLISAFADAKQQIRNKPQKSFNVNDEYICALRTKSYVEFFIKAQSVIEESSPPSTSS 91
           S+ + IS FAD K QIRNKPQKSFNVN+EY+CALR++S+VEFF KAQ +++E SPPSTSS
Sbjct: 8   SRFIWISVFADVKHQIRNKPQKSFNVNEEYLCALRSRSFVEFFAKAQLIVQE-SPPSTSS 67

Query: 92  STGRRRRRKLSETILLEPSQ-EAIPSILESPFLLMLPDLKSLLIDYFNVSAEASKFCTRL 151
           S      RK SETILL+  Q EA PSILESP L+MLP+L+ LLID+FNVSAEAS  C RL
Sbjct: 68  SAD-LYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASNLCHRL 127

Query: 152 LEDVELTRSNSRSIQKSLDSIENCSSPETIETIASEFLALREPFSDPDKHDFALIHDDYE 211
           L +++L RSNSR +QK LDSIE CSSP+ IETI S+ LA R P SD DK DFA IHDDY 
Sbjct: 128 LVNLKLARSNSRFVQKLLDSIEKCSSPKEIETITSDLLAQRAPLSDLDKRDFARIHDDYA 187

Query: 212 AVSRRLNCTRKKVARKIKSIEIIETISCGLVAITARTLTELFR----------FPPESFR 271
           AVSR LN TRKKVARKI+SI+II+  +CGLVAIT+R LT L            F  +S R
Sbjct: 188 AVSRSLNITRKKVARKIRSIKIIDRFTCGLVAITSRGLTALVMAADQGPVLRYFRLKSIR 247

Query: 272 RKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGKAMARLFV 331
           RKLLR+QM+ NGGL  VGEQLEAAAKGSYILNREFDTTSRLV RL DA+DHGKAM RLFV
Sbjct: 248 RKLLRHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFV 307

Query: 332 ERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVINQI 373
           ERKEDKFAV VAMDE+K+SN+ +R QVE+VEEHLYLC VTINR+R  VINQ+
Sbjct: 308 ERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCFVTINRSRASVINQM 357

BLAST of MS009218.1 vs. ExPASy Swiss-Prot
Match: A2XCJ1 (UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2)

HSP 1 Score: 123.6 bits (309), Expect = 4.6e-27
Identity = 109/349 (31.23%), Postives = 167/349 (47.85%), Query Frame = 0

Query: 51  PQKSFNVNDEYICALRTKSYVEFFIK--------AQSVIEESSPPSTSSSTGRRRRRKLS 110
           P  SF+  +EY  A RT+SY +F+ +          +++         +++ R    +L 
Sbjct: 28  PSSSFDFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLF 87

Query: 111 ETILLEPSQEAIPSILESPFLLML-PDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNS 170
              LLEP Q A+ + L SP    L PD++ LL  Y+  +A AS  C+ LL+D+E  R   
Sbjct: 88  AEHLLEPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRY 147

Query: 171 RSIQKSLDSIENCSSPETIETIASEFLALREPFSDPDKHDFAL--IHDDYEAVSRRLNCT 230
           R ++ +L  +   +S   +  +A    AL +PF+        L  +      + R L+  
Sbjct: 148 RPLKHTLRKL---ASDVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLDAG 207

Query: 231 RKKVARKIKSI-EIIETISCGLVAITA-----------RTLTELFRFPPESFRRKLLRYQ 290
           RKK   +I+S+  +   +S   V   A             L     FP  S     L  +
Sbjct: 208 RKKARHRIRSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMS--PAWLGER 267

Query: 291 MIRNGGLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGKAMARLFVERKEDKF 350
                       QLEAAAKG+YILNR+ +T SRLVAR+ D  +H  A+ RL VE +    
Sbjct: 268 FFSGRAARRALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALLRLCVEHRPAAG 327

Query: 351 A------VQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVIN 371
           A      VQ  + +L K+    R Q++E+EEHL+LC +TIN+AR +V+N
Sbjct: 328 AGGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTINKARIMVMN 371

BLAST of MS009218.1 vs. ExPASy Swiss-Prot
Match: Q10RR9 (UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.7e-26
Identity = 108/349 (30.95%), Postives = 166/349 (47.56%), Query Frame = 0

Query: 51  PQKSFNVNDEYICALRTKSYVEFFIK--------AQSVIEESSPPSTSSSTGRRRRRKLS 110
           P  SF+  +EY  A RT+SY +F+ +          +++         +++ R    +L 
Sbjct: 28  PSSSFDFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLF 87

Query: 111 ETILLEPSQEAIPSILESPFLLML-PDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNS 170
              LLEP Q A+ + L SP    L PD++ LL  Y+  +A AS  C+ LL+D+E  R   
Sbjct: 88  AEHLLEPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRY 147

Query: 171 RSIQKSLDSIENCSSPETIETIASEFLALREPFSDPDKHDFAL--IHDDYEAVSRRLNCT 230
           R ++ +L  +   +S   +  +A    AL +PF+        L  +      + R L+  
Sbjct: 148 RPLKHTLRKL---ASDVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLDAG 207

Query: 231 RKKVARKIKSI-EIIETISCGLVAITA-----------RTLTELFRFPPESFRRKLLRYQ 290
           RKK   +I+S+  +   +S   V   A             L     FP  S     L  +
Sbjct: 208 RKKARHRIRSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMS--PAWLGER 267

Query: 291 MIRNGGLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGKAMARLFVERKEDKF 350
                       QLEAAAKG+YILNR+ +T SRLVAR+ D  +H  A+ RL VE +    
Sbjct: 268 FFSGRAARRALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALRRLCVEHRPAAG 327

Query: 351 A------VQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVIN 371
           A      VQ  + +L K+    R Q++E+EEHL+LC +T N+AR +V+N
Sbjct: 328 AGGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTTNKARIMVMN 371

BLAST of MS009218.1 vs. ExPASy Swiss-Prot
Match: Q9SMU4 (UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.1e-25
Identity = 110/354 (31.07%), Postives = 171/354 (48.31%), Query Frame = 0

Query: 37  ISAFADAKQQIRNKPQKSFNVNDEYICALRTKSYVEFFIKAQSVIEE--------SSPPS 96
           +S+   A      K     +V +EY  A RT+SY  F+ +   +  +        SSPP 
Sbjct: 51  LSSIGTASGSSNPKDGADVDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPI 110

Query: 97  TSSSTGRR-RRRKLSETILLEPSQEAIPSILESPFLLMLPDLKSLLIDYFNVSAEASKFC 156
            SSST  R    +L    LL+P    I  IL+     +    ++LL DYF  +A A   C
Sbjct: 111 ESSSTSARLMSYRLFAHNLLDPDLNTITRILD--VSRVGRHTRTLLSDYFLETANAFLLC 170

Query: 157 TRLLEDVELTRSNSRSIQKSLDSIENCSSPETIETIASEFLALREPFSDPDKHDFALIHD 216
           T+LL+++   RS   S++    S EN +S   I+   +E     +PF         LI  
Sbjct: 171 TQLLKNIHHLRSKYESLKPKFHS-ENHNSLALIDQF-TEISKWFDPFISSGSR-IQLIRS 230

Query: 217 DYEAVSRRLNCTRKKVARKIKSIEIIETISCGLVAITARTLTELFRFPPESFRRKLLRYQ 276
               + +RL   R K   K+K I  + T S GL+ + A T T +      +F   L    
Sbjct: 231 GCLYLLKRLESRRDKTRAKLKLINGL-THSSGLL-VLALTTTLIVTIASHAFALFLAAPT 290

Query: 277 MIRN--------GGLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGKAMARLF 336
           ++ +          L +   +L+ AAKG+YIL+R+ DT SRLV R++D ++H +AMA  +
Sbjct: 291 LLASQFKPAGLRNKLTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFW 350

Query: 337 VERKEDKF-AVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVINQI 373
           V R   +    +    ELK+       +++E+EEH+YLC +TINRAR L++ +I
Sbjct: 351 VGRGSGRVRGSEEVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEI 397

BLAST of MS009218.1 vs. ExPASy Swiss-Prot
Match: Q6DYE5 (UPF0496 protein At1g20180 OS=Arabidopsis thaliana OX=3702 GN=At1g20180 PE=2 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 4.8e-24
Identity = 100/359 (27.86%), Postives = 179/359 (49.86%), Query Frame = 0

Query: 56  NVNDEYICALRTKSYVEFFIKAQSVIE-------ESSPPSTSSSTGRRRRRKLSETILLE 115
           +VN+EY  A RT SY+E   KA+  +         SS PS SSS+        ++  LL+
Sbjct: 35  SVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSHFTD-YLLD 94

Query: 116 PSQEAIPSILESPFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSL 175
           P QE + ++++         L +L++ +F++S+EA   C  LL+ ++  + N   I++ +
Sbjct: 95  PPQETLDALMQD------SSLDNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVM 154

Query: 176 DSIEN-CSSPETIETI-----------ASEFLALREPFSD-PDKHDFALIHDDYEAVSRR 235
              +  C+  +T+E              S F AL+ P     ++  F ++HD    +  +
Sbjct: 155 KIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTK 214

Query: 236 LNCTRKKVARKIKSIEIIE------------TISCGLVAITARTLTELFRFPPE----SF 295
           L   ++++ RKI+  +  +             I   L+ I   ++  +F  P      SF
Sbjct: 215 LTSKKRRIRRKIRFFKFCKKLGGYSLVITHSAIVITLLIIALHSILGVFAAPALLGLCSF 274

Query: 296 ---RRKLLRYQMIRNG---GLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGK 355
              R+K  + +M ++     L ++G Q++ AAKG +IL  + DT SRL  RL D ++H K
Sbjct: 275 CLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRK 334

Query: 356 AMARLFVERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVINQI 373
            +A +  + ++ +  ++ A+ E      +  +Q++E+EEHLYLC  TINR+R LV+ QI
Sbjct: 335 TVAAMCAKSRKIE-VLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 385

BLAST of MS009218.1 vs. ExPASy Swiss-Prot
Match: A2YH25 (Putative UPF0496 protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_023618 PE=3 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 1.7e-18
Identity = 69/264 (26.14%), Postives = 130/264 (49.24%), Query Frame = 0

Query: 129 LKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSLDSIENCSSPETIETIASEFL 188
           +++LLI+YF+V+ EA + C+ LL  +   R +  ++++ L  ++     +  + +A   +
Sbjct: 123 VETLLIEYFDVTEEACEACSALLAAIGAARRHHLTLRRLLLRLDGGDDDDAKDALA-RHV 182

Query: 189 ALREPFSDPDKHDFALIHDDYEAVSRRLNCTRKKVARKIKSIEIIE-TISCGLVAITART 248
            L  P S     +F  +H     ++ RL   ++++ R  +++ I   T +  LV   A  
Sbjct: 183 RLDNPLSPGSLSEFHDVHARCSPLASRLAAAQRRLRRLARALRIARGTAAAALVGACAAA 242

Query: 249 L-----------------TELFRFPPESFRRKLLR--YQMIRNGGLGEVGEQLEAAAKGS 308
           +                    F   P    R   R   + + +      G  L+AAA+G+
Sbjct: 243 IVAAVVLAAHALVGIGVAAAAFGATPAGAARWWARRAAEKVSSRHYARAGATLDAAARGA 302

Query: 309 YILNREFDTTSRLVARLDDAMDHGKAMARLFVERKEDKFAVQVAMDELKKSNLRLRNQVE 368
           YI+ R+ DT SR+V R  D ++HG+ +AR+ +    ++  +Q    E ++    LR Q+ 
Sbjct: 303 YIVGRDLDTVSRMVRRAHDELEHGRDVARIAMRGHGERPLLQEVAREEEECEEDLRAQLA 362

Query: 369 EVEEHLYLCIVTINRARGLVINQI 373
           E+EEH+ LC++TINR R LV +++
Sbjct: 363 ELEEHVCLCLITINRTRRLVAHEM 385

BLAST of MS009218.1 vs. ExPASy TrEMBL
Match: A0A6J1BWZ5 (UPF0496 protein 3-like OS=Momordica charantia OX=3673 GN=LOC111006097 PE=3 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 6.1e-200
Identity = 371/372 (99.73%), Postives = 371/372 (99.73%), Query Frame = 0

Query: 1   MWPKIRDLKIRNSTHISSSIFACSAAQLLNFSQPVLISAFADAKQQIRNKPQKSFNVNDE 60
           MWPKIRDLKIRNSTHISSSIFACSAAQLLNFSQPVLISAFADAKQQIRNKPQKSFNVNDE
Sbjct: 1   MWPKIRDLKIRNSTHISSSIFACSAAQLLNFSQPVLISAFADAKQQIRNKPQKSFNVNDE 60

Query: 61  YICALRTKSYVEFFIKAQSVIEESSPPSTSSSTGRRRRRKLSETILLEPSQEAIPSILES 120
           YICALRTKSYVEFFIKAQSVIEESSPPSTSSSTGRRRRRKLS TILLEPSQEAIPSILES
Sbjct: 61  YICALRTKSYVEFFIKAQSVIEESSPPSTSSSTGRRRRRKLSGTILLEPSQEAIPSILES 120

Query: 121 PFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSLDSIENCSSPETI 180
           PFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSLDSIENCSSPETI
Sbjct: 121 PFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSLDSIENCSSPETI 180

Query: 181 ETIASEFLALREPFSDPDKHDFALIHDDYEAVSRRLNCTRKKVARKIKSIEIIETISCGL 240
           ETIASEFLALREPFSDPDKHDFALIHDDYEAVSRRLNCTRKKVARKIKSIEIIETISCGL
Sbjct: 181 ETIASEFLALREPFSDPDKHDFALIHDDYEAVSRRLNCTRKKVARKIKSIEIIETISCGL 240

Query: 241 VAITARTLTELFRFPPESFRRKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSR 300
           VAITARTLTELFRFPPESFRRKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSR
Sbjct: 241 VAITARTLTELFRFPPESFRRKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSR 300

Query: 301 LVARLDDAMDHGKAMARLFVERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVT 360
           LVARLDDAMDHGKAMARLFVERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVT
Sbjct: 301 LVARLDDAMDHGKAMARLFVERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVT 360

Query: 361 INRARGLVINQI 373
           INRARGLVINQI
Sbjct: 361 INRARGLVINQI 372

BLAST of MS009218.1 vs. ExPASy TrEMBL
Match: A0A6J1JMU4 (UPF0496 protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111487265 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 4.1e-119
Identity = 245/352 (69.60%), Postives = 281/352 (79.83%), Query Frame = 0

Query: 32  SQPVLISAFADAKQQIRNKPQKSFNVNDEYICALRTKSYVEFFIKAQSVIEESSPPSTSS 91
           S+ + IS FAD K QIRNKPQKSFNVN+EY+CALR++S+VEFF KAQ +++E SPPST S
Sbjct: 8   SRFIWISVFADVKHQIRNKPQKSFNVNEEYLCALRSRSFVEFFAKAQLIVQE-SPPST-S 67

Query: 92  STGRRRRRKLSETILLEPSQ-EAIPSILESPFLLMLPDLKSLLIDYFNVSAEASKFCTRL 151
           STG    RK SETILL+  Q EA PSILESP L+MLP+L+ LLID+FNVSAEAS  C RL
Sbjct: 68  STG-HHCRKFSETILLQIDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASNLCHRL 127

Query: 152 LEDVELTRSNSRSIQKSLDSIENCSSPETIETIASEFLALREPFSDPDKHDFALIHDDYE 211
           L +++L RSNSR +QK LDSIE CSSP+ IETI S+ LA R P SD DK DFA IHDDY 
Sbjct: 128 LANLKLARSNSRFVQKLLDSIEKCSSPKEIETITSDLLAQRAPLSDLDKRDFARIHDDYA 187

Query: 212 AVSRRLNCTRKKVARKIKSIEIIETISCGLVAITARTLTELFR----------FPPESFR 271
           AVSR LN TRKKVARKI+SI+II   +CGLVAIT+R LT L            F  +S R
Sbjct: 188 AVSRSLNITRKKVARKIRSIKIINKCTCGLVAITSRGLTALVMAADQGPVLRYFRLKSIR 247

Query: 272 RKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGKAMARLFV 331
           RKLLR+QM+ NGGL  VGEQLEAAAKGSYILNREFDTTSRLV RL DA+DHGKAM RLFV
Sbjct: 248 RKLLRHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFV 307

Query: 332 ERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVINQI 373
           ERKEDKFAV VAMDE+K+SN+ +R QVE+VEEHLYLCIVTINR+R  VINQ+
Sbjct: 308 ERKEDKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 356

BLAST of MS009218.1 vs. ExPASy TrEMBL
Match: A0A6J1H6T3 (UPF0496 protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460642 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 4.1e-119
Identity = 243/352 (69.03%), Postives = 281/352 (79.83%), Query Frame = 0

Query: 32  SQPVLISAFADAKQQIRNKPQKSFNVNDEYICALRTKSYVEFFIKAQSVIEESSPPSTSS 91
           S+ + IS FAD K QIRNKPQKSFNVN+EY+CALR++S+VEFF KAQ +++E SPPSTSS
Sbjct: 8   SRFIWISVFADVKHQIRNKPQKSFNVNEEYLCALRSRSFVEFFAKAQLIVQE-SPPSTSS 67

Query: 92  STGRRRRRKLSETILLEPSQ-EAIPSILESPFLLMLPDLKSLLIDYFNVSAEASKFCTRL 151
           S      RK SETILL+  Q EA PSILESP L+MLP+L+ LLID+FNVSAEAS  C RL
Sbjct: 68  SAD-LYCRKFSETILLQSDQLEANPSILESPLLVMLPELRGLLIDFFNVSAEASNLCHRL 127

Query: 152 LEDVELTRSNSRSIQKSLDSIENCSSPETIETIASEFLALREPFSDPDKHDFALIHDDYE 211
           L +++L RSNSR +QK LDSIE CSSP+ IETI S+ LA R P SD DK DFA IHDDY 
Sbjct: 128 LANLKLARSNSRFVQKLLDSIEKCSSPKEIETITSDLLAQRAPLSDLDKRDFARIHDDYA 187

Query: 212 AVSRRLNCTRKKVARKIKSIEIIETISCGLVAITARTLTELFR----------FPPESFR 271
           AVSR LN TRKKVARKI+SI+II+  +CGLVAIT+R LT L            F  +S R
Sbjct: 188 AVSRSLNITRKKVARKIRSIKIIDRFTCGLVAITSRGLTALVMAADQGPVLRYFRLKSIR 247

Query: 272 RKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGKAMARLFV 331
           RKLLR+QM+ NGGL  VGEQLEAAAKGSYILNREFDTTSRLV RL DA+DHGKAM RLFV
Sbjct: 248 RKLLRHQMLGNGGLERVGEQLEAAAKGSYILNREFDTTSRLVVRLGDAVDHGKAMVRLFV 307

Query: 332 ERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVINQI 373
           ERKE+KFAV VAMDE+K+SN+ +R QVE+VEEHLYLCIVTINR+R  VINQ+
Sbjct: 308 ERKENKFAVAVAMDEMKRSNVSIRKQVEDVEEHLYLCIVTINRSRASVINQM 357

BLAST of MS009218.1 vs. ExPASy TrEMBL
Match: A0A5D3DGL5 (UPF0496 protein 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1163G00820 PE=3 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 2.4e-111
Identity = 229/376 (60.90%), Postives = 281/376 (74.73%), Query Frame = 0

Query: 1   MWPKIRDLKIRNSTHISSSIFACSAAQLLNFSQPVLISAFADAKQQ-IRNKPQKSFNVND 60
           MW K    KI NST +S SI AC              S FAD + + IR K ++SFNVN+
Sbjct: 1   MWTKFSPSKIINSTKLSQSILAC--------------STFADQEHEIIRKKHKRSFNVNE 60

Query: 61  EYICALRTKSYVEFFIKAQSVIEESSPPSTSSSTGRRRRRKLSETILLEPSQ-EAIPSIL 120
           EY+C LRT+S+ EFF+K +S + ES P +TSSS+      + SETILL+P Q EA+PSIL
Sbjct: 61  EYLCTLRTESFAEFFLKPESNVHESPPSTTSSSSAADCCWRFSETILLQPGQLEAVPSIL 120

Query: 121 ESPFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSLDSIENCSSPE 180
           ES FLLMLP+LK L +DYFN+SA+AS  CTRLL + +LTRS SR IQ+SLDSIE C S E
Sbjct: 121 ESSFLLMLPELKGLFVDYFNLSAQASDLCTRLLANFKLTRSTSRCIQESLDSIEKCFSSE 180

Query: 181 TIETIASEFLALREPFSDPDKHDFALIHDDYEAVSRRLNCTRKKVARKIKSIEIIETISC 240
           T+E+IAS  LALR PFSD +K DFALIHDDY  +S RLNCTRKKVARKI+S++I++ I+C
Sbjct: 181 TVESIASNLLALRSPFSDLEKRDFALIHDDYTTISHRLNCTRKKVARKIRSMKIMDGITC 240

Query: 241 GLVAITARTLTELFRFP---PESFRRKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREF 300
           GL AIT RTLT+L +     P  F RKLLR++M+RNGGL +VGE+LEAAAKGSYIL RE 
Sbjct: 241 GLNAITTRTLTDLVKAADRGPGVFGRKLLRHEMLRNGGLEKVGEKLEAAAKGSYILKREL 300

Query: 301 DTTSRLVARLDDAMDHGKAMARLFVER-KEDKFAVQVAMDELKKSNLRLRNQVEEVEEHL 360
           +TTSRLV RL DA+D+GKAM RLF  R KEDKF V VAMDE+KK+N  +R +VE+VEEHL
Sbjct: 301 ETTSRLVVRLGDAVDNGKAMVRLFGGRKKEDKFGVVVAMDEVKKNNANIRKRVEDVEEHL 360

Query: 361 YLCIVTINRARGLVIN 371
            LCIV INR++  +IN
Sbjct: 361 CLCIVAINRSKASMIN 362

BLAST of MS009218.1 vs. ExPASy TrEMBL
Match: A0A1S3B3L4 (UPF0496 protein 3-like OS=Cucumis melo OX=3656 GN=LOC103485615 PE=3 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 2.4e-111
Identity = 229/376 (60.90%), Postives = 281/376 (74.73%), Query Frame = 0

Query: 1   MWPKIRDLKIRNSTHISSSIFACSAAQLLNFSQPVLISAFADAKQQ-IRNKPQKSFNVND 60
           MW K    KI NST +S SI AC              S FAD + + IR K ++SFNVN+
Sbjct: 1   MWTKFSPSKIINSTKLSQSILAC--------------STFADQEHEIIRKKHKRSFNVNE 60

Query: 61  EYICALRTKSYVEFFIKAQSVIEESSPPSTSSSTGRRRRRKLSETILLEPSQ-EAIPSIL 120
           EY+C LRT+S+ EFF+K +S + ES P +TSSS+      + SETILL+P Q EA+PSIL
Sbjct: 61  EYLCTLRTESFAEFFLKPESNVHESPPSTTSSSSAADCCWRFSETILLQPGQLEAVPSIL 120

Query: 121 ESPFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSLDSIENCSSPE 180
           ES FLLMLP+LK L +DYFN+SA+AS  CTRLL + +LTRS SR IQ+SLDSIE C S E
Sbjct: 121 ESSFLLMLPELKGLFVDYFNLSAQASDLCTRLLANFKLTRSTSRCIQESLDSIEKCFSSE 180

Query: 181 TIETIASEFLALREPFSDPDKHDFALIHDDYEAVSRRLNCTRKKVARKIKSIEIIETISC 240
           T+E+IAS  LALR PFSD +K DFALIHDDY  +S RLNCTRKKVARKI+S++I++ I+C
Sbjct: 181 TVESIASNLLALRSPFSDLEKRDFALIHDDYTTISHRLNCTRKKVARKIRSMKIMDGITC 240

Query: 241 GLVAITARTLTELFRFP---PESFRRKLLRYQMIRNGGLGEVGEQLEAAAKGSYILNREF 300
           GL AIT RTLT+L +     P  F RKLLR++M+RNGGL +VGE+LEAAAKGSYIL RE 
Sbjct: 241 GLNAITTRTLTDLVKAADRGPGVFGRKLLRHEMLRNGGLEKVGEKLEAAAKGSYILKREL 300

Query: 301 DTTSRLVARLDDAMDHGKAMARLFVER-KEDKFAVQVAMDELKKSNLRLRNQVEEVEEHL 360
           +TTSRLV RL DA+D+GKAM RLF  R KEDKF V VAMDE+KK+N  +R +VE+VEEHL
Sbjct: 301 ETTSRLVVRLGDAVDNGKAMVRLFGGRKKEDKFGVVVAMDEVKKNNANIRKRVEDVEEHL 360

Query: 361 YLCIVTINRARGLVIN 371
            LCIV INR++  +IN
Sbjct: 361 CLCIVAINRSKASMIN 362

BLAST of MS009218.1 vs. TAIR 10
Match: AT3G49070.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 119.0 bits (297), Expect = 8.0e-27
Identity = 110/354 (31.07%), Postives = 171/354 (48.31%), Query Frame = 0

Query: 37  ISAFADAKQQIRNKPQKSFNVNDEYICALRTKSYVEFFIKAQSVIEE--------SSPPS 96
           +S+   A      K     +V +EY  A RT+SY  F+ +   +  +        SSPP 
Sbjct: 51  LSSIGTASGSSNPKDGADVDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPI 110

Query: 97  TSSSTGRR-RRRKLSETILLEPSQEAIPSILESPFLLMLPDLKSLLIDYFNVSAEASKFC 156
            SSST  R    +L    LL+P    I  IL+     +    ++LL DYF  +A A   C
Sbjct: 111 ESSSTSARLMSYRLFAHNLLDPDLNTITRILD--VSRVGRHTRTLLSDYFLETANAFLLC 170

Query: 157 TRLLEDVELTRSNSRSIQKSLDSIENCSSPETIETIASEFLALREPFSDPDKHDFALIHD 216
           T+LL+++   RS   S++    S EN +S   I+   +E     +PF         LI  
Sbjct: 171 TQLLKNIHHLRSKYESLKPKFHS-ENHNSLALIDQF-TEISKWFDPFISSGSR-IQLIRS 230

Query: 217 DYEAVSRRLNCTRKKVARKIKSIEIIETISCGLVAITARTLTELFRFPPESFRRKLLRYQ 276
               + +RL   R K   K+K I  + T S GL+ + A T T +      +F   L    
Sbjct: 231 GCLYLLKRLESRRDKTRAKLKLINGL-THSSGLL-VLALTTTLIVTIASHAFALFLAAPT 290

Query: 277 MIRN--------GGLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGKAMARLF 336
           ++ +          L +   +L+ AAKG+YIL+R+ DT SRLV R++D ++H +AMA  +
Sbjct: 291 LLASQFKPAGLRNKLTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFW 350

Query: 337 VERKEDKF-AVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVINQI 373
           V R   +    +    ELK+       +++E+EEH+YLC +TINRAR L++ +I
Sbjct: 351 VGRGSGRVRGSEEVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEI 397

BLAST of MS009218.1 vs. TAIR 10
Match: AT1G20180.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 113.6 bits (283), Expect = 3.4e-25
Identity = 100/359 (27.86%), Postives = 179/359 (49.86%), Query Frame = 0

Query: 56  NVNDEYICALRTKSYVEFFIKAQSVIE-------ESSPPSTSSSTGRRRRRKLSETILLE 115
           +VN+EY  A RT SY+E   KA+  +         SS PS SSS+        ++  LL+
Sbjct: 35  SVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSHFTD-YLLD 94

Query: 116 PSQEAIPSILESPFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSL 175
           P QE + ++++         L +L++ +F++S+EA   C  LL+ ++  + N   I++ +
Sbjct: 95  PPQETLDALMQD------SSLDNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVM 154

Query: 176 DSIEN-CSSPETIETI-----------ASEFLALREPFSD-PDKHDFALIHDDYEAVSRR 235
              +  C+  +T+E              S F AL+ P     ++  F ++HD    +  +
Sbjct: 155 KIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTK 214

Query: 236 LNCTRKKVARKIKSIEIIE------------TISCGLVAITARTLTELFRFPPE----SF 295
           L   ++++ RKI+  +  +             I   L+ I   ++  +F  P      SF
Sbjct: 215 LTSKKRRIRRKIRFFKFCKKLGGYSLVITHSAIVITLLIIALHSILGVFAAPALLGLCSF 274

Query: 296 ---RRKLLRYQMIRNG---GLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGK 355
              R+K  + +M ++     L ++G Q++ AAKG +IL  + DT SRL  RL D ++H K
Sbjct: 275 CLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRK 334

Query: 356 AMARLFVERKEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVINQI 373
            +A +  + ++ +  ++ A+ E      +  +Q++E+EEHLYLC  TINR+R LV+ QI
Sbjct: 335 TVAAMCAKSRKIE-VLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 385

BLAST of MS009218.1 vs. TAIR 10
Match: AT1G20180.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 113.6 bits (283), Expect = 3.4e-25
Identity = 97/340 (28.53%), Postives = 171/340 (50.29%), Query Frame = 0

Query: 56  NVNDEYICALRTKSYVEFFIKAQSVIE-------ESSPPSTSSSTGRRRRRKLSETILLE 115
           +VN+EY  A RT SY+E   KA+  +         SS PS SSS+        ++  LL+
Sbjct: 35  SVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSHFTD-YLLD 94

Query: 116 PSQEAIPSILESPFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSL 175
           P QE + ++++         L +L++ +F++S+EA   C  LL+ ++  + N   I++ +
Sbjct: 95  PPQETLDALMQD------SSLDNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVM 154

Query: 176 DSIEN-CSSPETIETI-----------ASEFLALREPFSD-PDKHDFALIHDDYEAVSRR 235
              +  C+  +T+E              S F AL+ P     ++  F ++HD    +  +
Sbjct: 155 KIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTK 214

Query: 236 LNCTRKKVARKIKSIEIIETISCGLVAITARTLTELFRFPPESFRRKLLRYQMIRNG--- 295
           L   ++++ RKI  + +            A  L  L  F     R+K  + +M ++    
Sbjct: 215 LTSKKRRIRRKISILGV----------FAAPALLGLCSFC--LLRKKKAKGRMHKSNKDT 274

Query: 296 GLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGKAMARLFVERKEDKFAVQVA 355
            L ++G Q++ AAKG +IL  + DT SRL  RL D ++H K +A +  + ++ +  ++ A
Sbjct: 275 TLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRKTVAAMCAKSRKIE-VLKEA 334

Query: 356 MDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVINQI 373
           + E      +  +Q++E+EEHLYLC  TINR+R LV+ QI
Sbjct: 335 LREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 354

BLAST of MS009218.1 vs. TAIR 10
Match: AT3G19330.3 (Protein of unknown function (DUF677) )

HSP 1 Score: 86.3 bits (212), Expect = 5.8e-17
Identity = 87/350 (24.86%), Postives = 161/350 (46.00%), Query Frame = 0

Query: 34  PVLISAFADAKQQIRNKPQKSFNVNDEYICALRTKSYVEFFIKAQSVIEESSPPSTSSST 93
           P L S  A   +   +    +FN++ E   A +T SY +   +   V++        +  
Sbjct: 24  PSLESGVASTSRNDASISSPTFNLSRELAHAFQTPSYHDVRSRVHVVVD-------LTQI 83

Query: 94  GRRRRRKLSETIL---LEPSQEAIPSILESPFLLMLPDLKSLLIDYFNVSAEASKFCTRL 153
             R  +   E +L   L+P++E +   +     +    L +L+  YF  S +A++ C  L
Sbjct: 84  HHRLIQPDIELLLSQVLQPNKECVQEAIRH---VKQTTLTNLVSTYFQHSEDATRLCLNL 143

Query: 154 LEDVELTRSNSRSIQKSLDSI-ENCSSPETIETIA----SEFLAL---REPFSDPDKHDF 213
            ++V   R +  +    L +I    S P   E++       FL L     PFS P+ + F
Sbjct: 144 YQNVHSARHHLYTPLLDLFNIFPGDSLPAIDESLCDLAFDVFLKLDTFENPFSSPESYSF 203

Query: 214 ALIHDDYEAVSRRLNCTRKKVARKIKSIEIIETISCGLVAITARTLTELFRFPPESFRRK 273
               D     S+  +   +++ +    + +I   + G +            + P SF+RK
Sbjct: 204 ---RDTQLCFSQLKHNLDRRLRKSRSRVRLIHHATAGPLCSP---------YLPHSFKRK 263

Query: 274 LLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGKAMARLFVER 333
            L               QL AA+KG+++LN++ DT  RLV+RL   +++ K + RL +ER
Sbjct: 264 ELTNIC-----------QLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLIRLGLER 323

Query: 334 KEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVINQI 373
             D  ++Q  +  L+KS+L L +Q++++E+H+ L    +N+AR L++ +I
Sbjct: 324 GRDVHSIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEI 340

BLAST of MS009218.1 vs. TAIR 10
Match: AT3G19330.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 86.3 bits (212), Expect = 5.8e-17
Identity = 87/350 (24.86%), Postives = 161/350 (46.00%), Query Frame = 0

Query: 34  PVLISAFADAKQQIRNKPQKSFNVNDEYICALRTKSYVEFFIKAQSVIEESSPPSTSSST 93
           P L S  A   +   +    +FN++ E   A +T SY +   +   V++        +  
Sbjct: 24  PSLESGVASTSRNDASISSPTFNLSRELAHAFQTPSYHDVRSRVHVVVD-------LTQI 83

Query: 94  GRRRRRKLSETIL---LEPSQEAIPSILESPFLLMLPDLKSLLIDYFNVSAEASKFCTRL 153
             R  +   E +L   L+P++E +   +     +    L +L+  YF  S +A++ C  L
Sbjct: 84  HHRLIQPDIELLLSQVLQPNKECVQEAIRH---VKQTTLTNLVSTYFQHSEDATRLCLNL 143

Query: 154 LEDVELTRSNSRSIQKSLDSI-ENCSSPETIETIA----SEFLAL---REPFSDPDKHDF 213
            ++V   R +  +    L +I    S P   E++       FL L     PFS P+ + F
Sbjct: 144 YQNVHSARHHLYTPLLDLFNIFPGDSLPAIDESLCDLAFDVFLKLDTFENPFSSPESYSF 203

Query: 214 ALIHDDYEAVSRRLNCTRKKVARKIKSIEIIETISCGLVAITARTLTELFRFPPESFRRK 273
               D     S+  +   +++ +    + +I   + G +            + P SF+RK
Sbjct: 204 ---RDTQLCFSQLKHNLDRRLRKSRSRVRLIHHATAGPLCSP---------YLPHSFKRK 263

Query: 274 LLRYQMIRNGGLGEVGEQLEAAAKGSYILNREFDTTSRLVARLDDAMDHGKAMARLFVER 333
            L               QL AA+KG+++LN++ DT  RLV+RL   +++ K + RL +ER
Sbjct: 264 ELTNIC-----------QLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLIRLGLER 323

Query: 334 KEDKFAVQVAMDELKKSNLRLRNQVEEVEEHLYLCIVTINRARGLVINQI 373
             D  ++Q  +  L+KS+L L +Q++++E+H+ L    +N+AR L++ +I
Sbjct: 324 GRDVHSIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEI 340