MS004214.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS004214.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	thioredoxin-like 3-2, chloroplastic
Location	scaffold92: 874472 .. 876136 (+)
Sequence length	588
RNA-Seq Expression	MS004214.1
Synteny	MS004214.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAAAGCTTTGCCCCTTCACCTCGGAACCGCGAAATCTTTTGCCAAATTCCACGCCCCCTTTGATCTTTCCACGCCTCCCCATCTTCCGGCCGGGAGGCTCGCCGGCTCCGGTTTCAACAGGCTTCCTTTGCCCACAAGGTTTCCTCCGCTTTCCCAGAAAGTTTCTGCCACTGGGGAGAAGACTTCGCTTCTCGGGGCTTGGACCCAGGAGAGTCCCTTGCAGTTTCGAGATGAATCCCCTGTGTCCATCGAGCTCAAGTCCATTTCTAGCGAACTTGAATTTGACCAGGCAATTTTAGATGCAGCGGAACGCGATGAGCTCGTGATTCTTGTATGGTATGTACGCGTTTTCTGGGAATTTTTTTTGTGTTGATCGTGGAAATTTCTTCCCCAGCGTTTAAATTGGTATTTCTTTTTGGAAGATCTAGAGGATGTGAATGTATTTGTTTTTGGCTGGATCTTATTTGATCAGAAGTTTACTTGGCCAGATTTTCCCTGGGGTTTCATTGAAACAGAATAACTTCCGTTGCACATTGATTGTGATAATTTTGAAGTGAATGATGCTTTATTTTCTCCAGTTTTCCTTACCAGTATTTTAAAGTACAATTCTCTTTATCTTCTTTTGTTGAAAAATGAGTCTGTGTGGATCTGTAGGATGGCTGACTGGTGCAGAAATTATATTTATTTAAAACCCCAATTGGAAAGATTGGCTGCAGATTATTACCCCAGGTTAGCTCTTTAGCCCATTCCTTCTGACCAAGTAAATGGTTAAAATTTGTACCATATTATCATTCTCCAACTGCTGGTGGATAATTCTAAAAAGACCAGTGTGCCAGTACTTACTGAAGTTGTGTACGTGCTTGATAAATTGTAGATTGCAATTCTACTGCATTGATGTCAATACGGTGCCACACAAGCTAGTTGTTCGTGCCGGGTTAGCTGTAAGTCCCTTTCCAAGCTTTTGATACTTATAATGATCTTCATAGGAAAATTGTTCTTCTGTAAGGAAATGATTTTCTTTGGATATTGTAACTATGAACTATCCCTTTGTATTGTACTTCTCTGAAATGTTGGGGTCTGGGAATTATAACAACCTGGTAGTTTCAGGTTTGGCTAAGTCCACCTTTAGATTTTGGCAATTAAATTTTCTGTTGTCATGGGGACTCCAGATTTCTGTTGACAGTAGAAGTTGGACTTCGGTTTCATTACCATTAGGAACATTTCCAATATGCATTTTGAACATCCTGGACAGAGGGCATGTTGAGCTTGTATTAGTTTTATGGATGAGTTGATTGAATAAGCTGCATAATCTGTTTTCTCTCTCATTTTAATGTGTGCAAACTTAAAATCTTTCCATCCTGGGTTAACTGCTACAAGTCCTCATAGTGTTATTTTTGTACATTTTCTCATGTATCTCAACCTTTTGTCGTTTCAATCTACCAGAAGATGCCAGCTATACAGGTGAGTTCCTTCACGATTACGTCTTACAATTGGAAATCTTCCTCTCTACTACTAATCACTAATGTTCTGAAGATCTTGATGACATTTGTTACAGCTATGGAAGGATGGTAAGAAGCAAGATGAGGTGATTGGTCTCTACAAAGCATATTTAGTCGCCAACGATGTTCAGAAAATGATCGAACGTGAGCTAACAGGCGAG

mRNA sequence

ATGGTGAAAGCTTTGCCCCTTCACCTCGGAACCGCGAAATCTTTTGCCAAATTCCACGCCCCCTTTGATCTTTCCACGCCTCCCCATCTTCCGGCCGGGAGGCTCGCCGGCTCCGGTTTCAACAGGCTTCCTTTGCCCACAAGGTTTCCTCCGCTTTCCCAGAAAGTTTCTGCCACTGGGGAGAAGACTTCGCTTCTCGGGGCTTGGACCCAGGAGAGTCCCTTGCAGTTTCGAGATGAATCCCCTGTGTCCATCGAGCTCAAGTCCATTTCTAGCGAACTTGAATTTGACCAGGCAATTTTAGATGCAGCGGAACGCGATGAGCTCGTGATTCTTGTATGGATGGCTGACTGGTGCAGAAATTATATTTATTTAAAACCCCAATTGGAAAGATTGGCTGCAGATTATTACCCCAGATTGCAATTCTACTGCATTGATGTCAATACGGTGCCACACAAGCTAGTTGTTCGTGCCGGGTTAGCTCTATGGAAGGATGGTAAGAAGCAAGATGAGGTGATTGGTCTCTACAAAGCATATTTAGTCGCCAACGATGTTCAGAAAATGATCGAACGTGAGCTAACAGGCGAG

Coding sequence (CDS)

ATGGTGAAAGCTTTGCCCCTTCACCTCGGAACCGCGAAATCTTTTGCCAAATTCCACGCCCCCTTTGATCTTTCCACGCCTCCCCATCTTCCGGCCGGGAGGCTCGCCGGCTCCGGTTTCAACAGGCTTCCTTTGCCCACAAGGTTTCCTCCGCTTTCCCAGAAAGTTTCTGCCACTGGGGAGAAGACTTCGCTTCTCGGGGCTTGGACCCAGGAGAGTCCCTTGCAGTTTCGAGATGAATCCCCTGTGTCCATCGAGCTCAAGTCCATTTCTAGCGAACTTGAATTTGACCAGGCAATTTTAGATGCAGCGGAACGCGATGAGCTCGTGATTCTTGTATGGATGGCTGACTGGTGCAGAAATTATATTTATTTAAAACCCCAATTGGAAAGATTGGCTGCAGATTATTACCCCAGATTGCAATTCTACTGCATTGATGTCAATACGGTGCCACACAAGCTAGTTGTTCGTGCCGGGTTAGCTCTATGGAAGGATGGTAAGAAGCAAGATGAGGTGATTGGTCTCTACAAAGCATATTTAGTCGCCAACGATGTTCAGAAAATGATCGAACGTGAGCTAACAGGCGAG

Protein sequence

MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATGEKTSLLGAWTQESPLQFRDESPVSIELKSISSELEFDQAILDAAERDELVILVWMADWCRNYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLALWKDGKKQDEVIGLYKAYLVANDVQKMIERELTGE

Homology

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BLAST of MS004214.1 vs. NCBI nr
Match: XP_022138917.1 (thioredoxin-like 3-2, chloroplastic [Momordica charantia] >XP_022138918.1 thioredoxin-like 3-2, chloroplastic [Momordica charantia] >XP_022138919.1 thioredoxin-like 3-2, chloroplastic [Momordica charantia])

HSP 1 Score: 387.9 bits (995), Expect = 5.2e-104
Identity = 194/202 (96.04%), Postives = 194/202 (96.04%), Query Frame = 0


Query: 1   MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG 60
           MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG
Sbjct: 1   MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG 60

Query: 61  EKTSLLGAWTQESPLQFRDESPVSIELKSISSELEFDQAILDAAERDELVILVWMADWCR 120
           EKTSLLGAWTQESPLQFRDESPVSIELKSISSE EFDQAILDAAERDELVILVWMADWCR
Sbjct: 61  EKTSLLGAWTQESPLQFRDESPVSIELKSISSEHEFDQAILDAAERDELVILVWMADWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLA------LWKDGKKQDEVIG 180
           NYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLA      LWKDGKKQDEVIG
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIG 180

Query: 181 LYKAYLVANDVQKMIERELTGE 197
           LYKAYLVANDVQKMIE ELTGE
Sbjct: 181 LYKAYLVANDVQKMIECELTGE 202

[Close]

BLAST of MS004214.1 vs. NCBI nr
Match: XP_038891539.1 (thioredoxin-like 3-2, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 336.3 bits (861), Expect = 1.8e-88
Identity = 171/202 (84.65%), Postives = 178/202 (88.12%), Query Frame = 0


Query: 1   MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG 60
           MVKALPLHLG  KSF KF   FDL  PP LP+ RLAGSGFNRLPL  +FPPLSQKVSATG
Sbjct: 1   MVKALPLHLGIVKSFPKFDGSFDLPPPPQLPSARLAGSGFNRLPLCRKFPPLSQKVSATG 60

Query: 61  EK-TSLLGAWTQESPLQFRDESPVSIELKSISSELEFDQAILDAAERDELVILVWMADWC 120
           EK TSLLGAWTQ+S LQFRDESPVSIELKSI SELEFD+AI DA +R+ELVILVWMA+WC
Sbjct: 61  EKTTSLLGAWTQDSTLQFRDESPVSIELKSICSELEFDKAIADAEKRNELVILVWMANWC 120

Query: 121 RNYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLA-----LWKDGKKQDEVIG 180
           RNYIYLKPQLERLAADYYPRLQFYCIDVN VPHKLVVRAGLA     LWKDGKKQDEVIG
Sbjct: 121 RNYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAMPAIQLWKDGKKQDEVIG 180

Query: 181 LYKAYLVANDVQKMIERELTGE 197
           LYKAYLV NDVQKMIERELTGE
Sbjct: 181 LYKAYLVINDVQKMIERELTGE 202

[Close]

BLAST of MS004214.1 vs. NCBI nr
Match: XP_038891538.1 (thioredoxin-like 3-2, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 335.9 bits (860), Expect = 2.4e-88
Identity = 171/203 (84.24%), Postives = 178/203 (87.68%), Query Frame = 0


Query: 1   MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG 60
           MVKALPLHLG  KSF KF   FDL  PP LP+ RLAGSGFNRLPL  +FPPLSQKVSATG
Sbjct: 1   MVKALPLHLGIVKSFPKFDGSFDLPPPPQLPSARLAGSGFNRLPLCRKFPPLSQKVSATG 60

Query: 61  EK-TSLLGAWTQESPLQFRDESPVSIELKSISSELEFDQAILDAAERDELVILVWMADWC 120
           EK TSLLGAWTQ+S LQFRDESPVSIELKSI SELEFD+AI DA +R+ELVILVWMA+WC
Sbjct: 61  EKTTSLLGAWTQDSTLQFRDESPVSIELKSICSELEFDKAIADAEKRNELVILVWMANWC 120

Query: 121 RNYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLA------LWKDGKKQDEVI 180
           RNYIYLKPQLERLAADYYPRLQFYCIDVN VPHKLVVRAGLA      LWKDGKKQDEVI
Sbjct: 121 RNYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVI 180

Query: 181 GLYKAYLVANDVQKMIERELTGE 197
           GLYKAYLV NDVQKMIERELTGE
Sbjct: 181 GLYKAYLVINDVQKMIERELTGE 203

[Close]

BLAST of MS004214.1 vs. NCBI nr
Match: XP_008446853.1 (PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X9 [Cucumis melo])

HSP 1 Score: 329.3 bits (843), Expect = 2.2e-86
Identity = 165/201 (82.09%), Postives = 175/201 (87.06%), Query Frame = 0


Query: 1   MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG 60
           MVKALPLHLG  KSF KF + FDL   P L + RL GSGFNRLPL  +FPP+ QKVSATG
Sbjct: 1   MVKALPLHLGIVKSFPKFDSSFDLPPSPQLLSARLPGSGFNRLPLSRKFPPIFQKVSATG 60

Query: 61  EKTSLLGAWTQESPLQFRDESPVSIELKSISSELEFDQAILDAAERDELVILVWMADWCR 120
           EKTSLLGAWTQ+S LQFRDESPVSIELKSISSELEFD+AI DA ER+ELVILVWMA+WCR
Sbjct: 61  EKTSLLGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLA-----LWKDGKKQDEVIGL 180
           NYIYLKPQLERLAADYYPRLQFYCIDVN VPHKLVVRAGLA     LWKDGKKQDEV+GL
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAMPAIQLWKDGKKQDEVVGL 180

Query: 181 YKAYLVANDVQKMIERELTGE 197
           YKAYLV NDV+KMIERELT E
Sbjct: 181 YKAYLVVNDVRKMIERELTDE 201

[Close]

BLAST of MS004214.1 vs. NCBI nr
Match: XP_008446852.1 (PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X8 [Cucumis melo] >TYK09259.1 thioredoxin-like 3-2 [Cucumis melo var. makuwa])

HSP 1 Score: 328.9 bits (842), Expect = 2.9e-86
Identity = 165/202 (81.68%), Postives = 175/202 (86.63%), Query Frame = 0


Query: 1   MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG 60
           MVKALPLHLG  KSF KF + FDL   P L + RL GSGFNRLPL  +FPP+ QKVSATG
Sbjct: 1   MVKALPLHLGIVKSFPKFDSSFDLPPSPQLLSARLPGSGFNRLPLSRKFPPIFQKVSATG 60

Query: 61  EKTSLLGAWTQESPLQFRDESPVSIELKSISSELEFDQAILDAAERDELVILVWMADWCR 120
           EKTSLLGAWTQ+S LQFRDESPVSIELKSISSELEFD+AI DA ER+ELVILVWMA+WCR
Sbjct: 61  EKTSLLGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLA------LWKDGKKQDEVIG 180
           NYIYLKPQLERLAADYYPRLQFYCIDVN VPHKLVVRAGLA      LWKDGKKQDEV+G
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVVG 180

Query: 181 LYKAYLVANDVQKMIERELTGE 197
           LYKAYLV NDV+KMIERELT E
Sbjct: 181 LYKAYLVVNDVRKMIERELTDE 202

[Close]

BLAST of MS004214.1 vs. ExPASy Swiss-Prot
Match: Q8VZT6 (Thioredoxin-like 3-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=WCRKC2 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.7e-36
Identity = 79/171 (46.20%), Postives = 111/171 (64.91%), Query Frame = 0


Query: 28  PHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATGEKTSLLGAWTQESPLQFRDESPVSIEL 87
           P +P  R   S F+R P   ++   + K+    E+   +    Q+  LQ  D+SPVS+EL
Sbjct: 20  PLVPPYRQTSSSFSR-PRNFKYHSFTDKICLAAERIRAVDIQKQDGGLQELDDSPVSVEL 79

Query: 88  KSISSELEFDQAILDAAERDELVILVWMADWCRNYIYLKPQLERLAADYYPRLQFYCIDV 147
             I  E  FDQ + DA +  E V++VWMA WCR  IYLKP+LE+LAA++YPRL+FY +DV
Sbjct: 80  GPICGESHFDQVMEDAQKLGESVVIVWMAAWCRKCIYLKPKLEKLAAEFYPRLRFYHVDV 139

Query: 148 NTVPHKLVVRAG------LALWKDGKKQDEVIGLYKAYLVANDVQKMIERE 193
           N VP++LV RAG      + LW+DG+KQ EVIG +KA+ V N+V++MIE +
Sbjct: 140 NAVPYRLVSRAGVTKMPTIQLWRDGQKQAEVIGGHKAHFVVNEVREMIEND 189

[Close]

BLAST of MS004214.1 vs. ExPASy Swiss-Prot
Match: Q9FG36 (Thioredoxin-like 3-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=WCRKC1 PE=2 SV=3)

HSP 1 Score: 100.5 bits (249), Expect = 2.2e-20
Identity = 47/116 (40.52%), Postives = 76/116 (65.52%), Query Frame = 0


Query: 82  PVSIELKSISSELEFDQAILDAAERDELVILVWMADWCRNYIYLKPQLERLAADYYPRLQ 141
           P ++E++ I++  E D  +  A +  + +I+ WMA WCR  IYLKP+LE+LAA+Y  R +
Sbjct: 93  PTTLEMEPINNVEELDAVLSHARQLSQPIIIEWMASWCRKCIYLKPKLEKLAAEYNNRAK 152

Query: 142 FYCIDVNTVPHKLVVRAGLA------LWKDGKKQDEVIGLYKAYLVANDVQKMIER 192
           FY +DVN VP  LV R  ++      LWK+ + ++EVIG +K +LV  +V+++I +
Sbjct: 153 FYYVDVNKVPQTLVKRGNISKMPTIQLWKEDEMKEEVIGGHKGWLVIEEVRELINK 208

[Close]

BLAST of MS004214.1 vs. ExPASy Swiss-Prot
Match: Q6Z7L3 (Thioredoxin-like 3-1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0774100 PE=3 SV=2)

HSP 1 Score: 96.3 bits (238), Expect = 4.1e-19
Identity = 47/113 (41.59%), Postives = 75/113 (66.37%), Query Frame = 0


Query: 85  IELKSISSELEFDQAILDAAERDELVILVWMADWCRNYIYLKPQLERLAADYYPRLQFYC 144
           +E++ I S  + D A+  + E  + +I+ WMA WCR  IYLKP+LE++A + +P ++FY 
Sbjct: 80  VEMEEIDSMEKLDDALRWSVENKQPIIIDWMASWCRKCIYLKPRLEKIAGE-FPGVRFYF 139

Query: 145 IDVNTVPHKLVVRAGLA------LWKDGKKQDEVIGLYKAYLVANDVQKMIER 192
           +DVN VP  +V R  +       LWKDG+   EVIG +KA+LV ++V++MI++
Sbjct: 140 VDVNKVPQTVVKRGNITKMPTIQLWKDGEWAAEVIGGHKAWLVMDEVREMIQK 191

[Close]

BLAST of MS004214.1 vs. ExPASy TrEMBL
Match: A0A6J1CBF6 (thioredoxin-like 3-2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111009980 PE=4 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 2.5e-104
Identity = 194/202 (96.04%), Postives = 194/202 (96.04%), Query Frame = 0


Query: 1   MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG 60
           MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG
Sbjct: 1   MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG 60

Query: 61  EKTSLLGAWTQESPLQFRDESPVSIELKSISSELEFDQAILDAAERDELVILVWMADWCR 120
           EKTSLLGAWTQESPLQFRDESPVSIELKSISSE EFDQAILDAAERDELVILVWMADWCR
Sbjct: 61  EKTSLLGAWTQESPLQFRDESPVSIELKSISSEHEFDQAILDAAERDELVILVWMADWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLA------LWKDGKKQDEVIG 180
           NYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLA      LWKDGKKQDEVIG
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIG 180

Query: 181 LYKAYLVANDVQKMIERELTGE 197
           LYKAYLVANDVQKMIE ELTGE
Sbjct: 181 LYKAYLVANDVQKMIECELTGE 202

[Close]

BLAST of MS004214.1 vs. ExPASy TrEMBL
Match: A0A1S3BGW0 (thioredoxin-like 3-2, chloroplastic isoform X9 OS=Cucumis melo OX=3656 GN=LOC103489448 PE=4 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.1e-86
Identity = 165/201 (82.09%), Postives = 175/201 (87.06%), Query Frame = 0


Query: 1   MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG 60
           MVKALPLHLG  KSF KF + FDL   P L + RL GSGFNRLPL  +FPP+ QKVSATG
Sbjct: 1   MVKALPLHLGIVKSFPKFDSSFDLPPSPQLLSARLPGSGFNRLPLSRKFPPIFQKVSATG 60

Query: 61  EKTSLLGAWTQESPLQFRDESPVSIELKSISSELEFDQAILDAAERDELVILVWMADWCR 120
           EKTSLLGAWTQ+S LQFRDESPVSIELKSISSELEFD+AI DA ER+ELVILVWMA+WCR
Sbjct: 61  EKTSLLGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLA-----LWKDGKKQDEVIGL 180
           NYIYLKPQLERLAADYYPRLQFYCIDVN VPHKLVVRAGLA     LWKDGKKQDEV+GL
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAMPAIQLWKDGKKQDEVVGL 180

Query: 181 YKAYLVANDVQKMIERELTGE 197
           YKAYLV NDV+KMIERELT E
Sbjct: 181 YKAYLVVNDVRKMIERELTDE 201

[Close]

BLAST of MS004214.1 vs. ExPASy TrEMBL
Match: A0A5D3CG32 (Thioredoxin-like 3-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002530 PE=4 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.4e-86
Identity = 165/202 (81.68%), Postives = 175/202 (86.63%), Query Frame = 0


Query: 1   MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG 60
           MVKALPLHLG  KSF KF + FDL   P L + RL GSGFNRLPL  +FPP+ QKVSATG
Sbjct: 1   MVKALPLHLGIVKSFPKFDSSFDLPPSPQLLSARLPGSGFNRLPLSRKFPPIFQKVSATG 60

Query: 61  EKTSLLGAWTQESPLQFRDESPVSIELKSISSELEFDQAILDAAERDELVILVWMADWCR 120
           EKTSLLGAWTQ+S LQFRDESPVSIELKSISSELEFD+AI DA ER+ELVILVWMA+WCR
Sbjct: 61  EKTSLLGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLA------LWKDGKKQDEVIG 180
           NYIYLKPQLERLAADYYPRLQFYCIDVN VPHKLVVRAGLA      LWKDGKKQDEV+G
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVVG 180

Query: 181 LYKAYLVANDVQKMIERELTGE 197
           LYKAYLV NDV+KMIERELT E
Sbjct: 181 LYKAYLVVNDVRKMIERELTDE 202

[Close]

BLAST of MS004214.1 vs. ExPASy TrEMBL
Match: A0A1S3BGQ4 (thioredoxin-like 3-2, chloroplastic isoform X8 OS=Cucumis melo OX=3656 GN=LOC103489448 PE=4 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.4e-86
Identity = 165/202 (81.68%), Postives = 175/202 (86.63%), Query Frame = 0


Query: 1   MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG 60
           MVKALPLHLG  KSF KF + FDL   P L + RL GSGFNRLPL  +FPP+ QKVSATG
Sbjct: 1   MVKALPLHLGIVKSFPKFDSSFDLPPSPQLLSARLPGSGFNRLPLSRKFPPIFQKVSATG 60

Query: 61  EKTSLLGAWTQESPLQFRDESPVSIELKSISSELEFDQAILDAAERDELVILVWMADWCR 120
           EKTSLLGAWTQ+S LQFRDESPVSIELKSISSELEFD+AI DA ER+ELVILVWMA+WCR
Sbjct: 61  EKTSLLGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLA------LWKDGKKQDEVIG 180
           NYIYLKPQLERLAADYYPRLQFYCIDVN VPHKLVVRAGLA      LWKDGKKQDEV+G
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVVG 180

Query: 181 LYKAYLVANDVQKMIERELTGE 197
           LYKAYLV NDV+KMIERELT E
Sbjct: 181 LYKAYLVVNDVRKMIERELTDE 202

[Close]

BLAST of MS004214.1 vs. ExPASy TrEMBL
Match: A0A0A0KUX7 (Thioredoxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G622670 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 4.1e-86
Identity = 165/202 (81.68%), Postives = 174/202 (86.14%), Query Frame = 0


Query: 1   MVKALPLHLGTAKSFAKFHAPFDLSTPPHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATG 60
           MVKALPLH G  KSF KF + FDL   P L + RLAGSGFNRLPL  +FPPL QKVSATG
Sbjct: 1   MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG 60

Query: 61  EKTSLLGAWTQESPLQFRDESPVSIELKSISSELEFDQAILDAAERDELVILVWMADWCR 120
           EKTSL GAWTQ+S LQFRDESPVSIELKSISSELEFD+AI DA ER+ELVILVWMA+WCR
Sbjct: 61  EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNTVPHKLVVRAGLA------LWKDGKKQDEVIG 180
           NYIYLKPQLERLAADYYPRLQFYCIDVN VPHKLVVRAGLA      LWKDGKKQDEV+G
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLAKMPAIQLWKDGKKQDEVVG 180

Query: 181 LYKAYLVANDVQKMIERELTGE 197
           LYKAYLV NDV+KMIERELT E
Sbjct: 181 LYKAYLVVNDVRKMIERELTDE 202

[Close]

BLAST of MS004214.1 vs. TAIR 10
Match: AT5G04260.1 (WCRKC thioredoxin 2 )

HSP 1 Score: 152.9 bits (385), Expect = 2.6e-37
Identity = 79/171 (46.20%), Postives = 111/171 (64.91%), Query Frame = 0


Query: 28  PHLPAGRLAGSGFNRLPLPTRFPPLSQKVSATGEKTSLLGAWTQESPLQFRDESPVSIEL 87
           P +P  R   S F+R P   ++   + K+    E+   +    Q+  LQ  D+SPVS+EL
Sbjct: 20  PLVPPYRQTSSSFSR-PRNFKYHSFTDKICLAAERIRAVDIQKQDGGLQELDDSPVSVEL 79

Query: 88  KSISSELEFDQAILDAAERDELVILVWMADWCRNYIYLKPQLERLAADYYPRLQFYCIDV 147
             I  E  FDQ + DA +  E V++VWMA WCR  IYLKP+LE+LAA++YPRL+FY +DV
Sbjct: 80  GPICGESHFDQVMEDAQKLGESVVIVWMAAWCRKCIYLKPKLEKLAAEFYPRLRFYHVDV 139

Query: 148 NTVPHKLVVRAG------LALWKDGKKQDEVIGLYKAYLVANDVQKMIERE 193
           N VP++LV RAG      + LW+DG+KQ EVIG +KA+ V N+V++MIE +
Sbjct: 140 NAVPYRLVSRAGVTKMPTIQLWRDGQKQAEVIGGHKAHFVVNEVREMIEND 189

[Close]

BLAST of MS004214.1 vs. TAIR 10
Match: AT5G06690.1 (WCRKC thioredoxin 1 )

HSP 1 Score: 100.5 bits (249), Expect = 1.6e-21
Identity = 47/116 (40.52%), Postives = 76/116 (65.52%), Query Frame = 0


Query: 82  PVSIELKSISSELEFDQAILDAAERDELVILVWMADWCRNYIYLKPQLERLAADYYPRLQ 141
           P ++E++ I++  E D  +  A +  + +I+ WMA WCR  IYLKP+LE+LAA+Y  R +
Sbjct: 93  PTTLEMEPINNVEELDAVLSHARQLSQPIIIEWMASWCRKCIYLKPKLEKLAAEYNNRAK 152

Query: 142 FYCIDVNTVPHKLVVRAGLA------LWKDGKKQDEVIGLYKAYLVANDVQKMIER 192
           FY +DVN VP  LV R  ++      LWK+ + ++EVIG +K +LV  +V+++I +
Sbjct: 153 FYYVDVNKVPQTLVKRGNISKMPTIQLWKEDEMKEEVIGGHKGWLVIEEVRELINK 208

[Close]

BLAST of MS004214.1 vs. TAIR 10
Match: AT5G06690.2 (WCRKC thioredoxin 1 )

HSP 1 Score: 79.7 bits (195), Expect = 2.8e-15
Identity = 35/81 (43.21%), Postives = 54/81 (66.67%), Query Frame = 0


Query: 82  PVSIELKSISSELEFDQAILDAAERDELVILVWMADWCRNYIYLKPQLERLAADYYPRLQ 141
           P ++E++ I++  E D  +  A +  + +I+ WMA WCR  IYLKP+LE+LAA+Y  R +
Sbjct: 93  PTTLEMEPINNVEELDAVLSHARQLSQPIIIEWMASWCRKCIYLKPKLEKLAAEYNNRAK 152

Query: 142 FYCIDVNTVPHKLVVRAGLAL 163
           FY +DVN VP  LV R  +++
Sbjct: 153 FYYVDVNKVPQTLVKRGNISV 173

The following BLAST results are available for this feature:

BLAST of MS004214.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138917.1	5.2e-104	96.04	thioredoxin-like 3-2, chloroplastic [Momordica charantia] >XP_022138918.1 thiore...	[more]
XP_038891539.1	1.8e-88	84.65	thioredoxin-like 3-2, chloroplastic isoform X2 [Benincasa hispida]	[more]
XP_038891538.1	2.4e-88	84.24	thioredoxin-like 3-2, chloroplastic isoform X1 [Benincasa hispida]	[more]
XP_008446853.1	2.2e-86	82.09	PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X9 [Cucumis melo]	[more]
XP_008446852.1	2.9e-86	81.68	PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X8 [Cucumis melo] >TYK092...	[more]

BLAST of MS004214.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VZT6	3.7e-36	46.20	Thioredoxin-like 3-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=WCRKC2 PE...	[more]
Q9FG36	2.2e-20	40.52	Thioredoxin-like 3-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=WCRKC1 PE...	[more]
Q6Z7L3	4.1e-19	41.59	Thioredoxin-like 3-1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]

BLAST of MS004214.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CBF6	2.5e-104	96.04	thioredoxin-like 3-2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC1110099...	[more]
A0A1S3BGW0	1.1e-86	82.09	thioredoxin-like 3-2, chloroplastic isoform X9 OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A5D3CG32	1.4e-86	81.68	Thioredoxin-like 3-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475...	[more]
A0A1S3BGQ4	1.4e-86	81.68	thioredoxin-like 3-2, chloroplastic isoform X8 OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A0A0KUX7	4.1e-86	81.68	Thioredoxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G622670...	[more]

BLAST of MS004214.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G04260.1	2.6e-37	46.20	WCRKC thioredoxin 2	[more]
AT5G06690.1	1.6e-21	40.52	WCRKC thioredoxin 1	[more]
AT5G06690.2	2.8e-15	43.21	WCRKC thioredoxin 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013766	Thioredoxin domain	PFAM	PF00085	Thioredoxin	coord: 93..177 e-value: 3.2E-5 score: 23.8
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 78..188 e-value: 6.3E-9 score: 37.5
None	No IPR available	PANTHER	PTHR47192:SF4	THIOREDOXIN-LIKE 3-2, CHLOROPLASTIC	coord: 49..193
None	No IPR available	CDD	cd02947	TRX_family	coord: 95..190 e-value: 1.60678E-10 score: 53.7161
IPR044253	Thioredoxin-like 3-1/3-2	PANTHER	PTHR47192	THIOREDOXIN-LIKE 3-2, CHLOROPLASTIC	coord: 49..193
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 87..189

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS004214	MS004214	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS004214.1-cds	MS004214.1-cds-scaffold92:874472..874812	CDS
MS004214.1-cds	MS004214.1-cds-scaffold92:875131..875205	CDS
MS004214.1-cds	MS004214.1-cds-scaffold92:875351..875417	CDS
MS004214.1-cds	MS004214.1-cds-scaffold92:876032..876136	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS004214.1	MS004214.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009570	chloroplast stroma