MC11g1207.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC11g1207.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	zf-RVT domain-containing protein
Location	MC11: 11773868 .. 11774358 (+)
Sequence length	480
RNA-Seq Expression	MC11g1207.1
Synteny	MC11g1207.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAACCAATTGTCCTAAAAGAATGAACATTCTGATCTGGATTCTTTTAAACAGCAGCAGCATTAATGCGATGGATGTTCTTAAATGGAAGCTTTCTTATTCTCAGCTTTTGCCTTCAGTTTGTTTCCTCTGTTTTGCAGATTCTGAAAACCAAAATCACCTCCTTTTTGGCTGTTCTTTGTCCCTGAAGTGCTGGTTCTGGGTTTTAAAAAAGTTTTGGATTCTTTGGGCCTTCTCAAATGACTACAAGAATAATGTTTTGAATCTCTTGGTTGGGCCGTCTCTATCACCAAAAGCTCATCCAACAAAGTCAAAGCTAATAGTTCAGAATTATGGTTGGAACGTAACCATATAATCTTTCAAAAAAAGATTTGCTGCTCATTGGATCAGTTTGACATGGCTTCTTAAAGAGCCTCACAGTAGCGTTCTCTTTCTAATACTTTTTTGGACTATAATTTTGTTGATATTTCCTTAAACTGGGAAGCATTTATT

mRNA sequence

AAAACCAATTGTCCTAAAAGAATGAACATTCTGATCTGGATTCTTTTAAACAGCAGCAGCATTAATGCGATGGATGTTCTTAAATGGAAGCTTTCTTATTCTCAGCTTTTGCCTTCAGTTTGTTTCCTCTGTTTTGCAGATTCTGAAAACCAAAATCACCTCCTTTTTGGCTGTTCTTTGTCCCTGAAGTGCTGGTTCTGGGTTTTAAAAAAGTTTTGGATTCTTTGGGCCTTCTCAAATGACTACAAGAATAATGTTTTGAATCTCTTGGTTGGGCCGTCTCTAAAAAGCTCATCCAACAAAGTCAAAGCTAATAGTTCAGAATTATGGTTGGAACGTAACCATATAATCTTTCAAAAAAAGATTTGCTGCTCATTGGATCAGTTTGACATGGCTTTAAAGAGCCTCACACGTTCTCTTTCTAATACTTTTTTGGACTATAATTTTGTTGATATTTCCTTAAACTGGGAAGCATTTATT

Coding sequence (CDS)

AAAACCAATTGTCCTAAAAGAATGAACATTCTGATCTGGATTCTTTTAAACAGCAGCAGCATTAATGCGATGGATGTTCTTAAATGGAAGCTTTCTTATTCTCAGCTTTTGCCTTCAGTTTGTTTCCTCTGTTTTGCAGATTCTGAAAACCAAAATCACCTCCTTTTTGGCTGTTCTTTGTCCCTGAAGTGCTGGTTCTGGGTTTTAAAAAAGTTTTGGATTCTTTGGGCCTTCTCAAATGACTACAAGAATAATGTTTTGAATCTCTTGGTTGGGCCGTCTCTAAAAAGCTCATCCAACAAAGTCAAAGCTAATAGTTCAGAATTATGGTTGGAACGTAACCATATAATCTTTCAAAAAAAGATTTGCTGCTCATTGGATCAGTTTGACATGGCTTTAAAGAGCCTCACACGTTCTCTTTCTAATACTTTTTTGGACTATAATTTTGTTGATATTTCCTTAAACTGGGAAGCATTTATT

Protein sequence

KTNCPKRMNILIWILLNSSSINAMDVLKWKLSYSQLLPSVCFLCFADSENQNHLLFGCSLSLKCWFWVLKKFWILWAFSNDYKNNVLNLLVGPSLKSSSNKVKANSSELWLERNHIIFQKKICCSLDQFDMALKSLTRSLSNTFLDYNFVDISLNWEAFI

Homology

[Close]

BLAST of MC11g1207.1 vs. NCBI nr
Match: XP_038903695.1 (uncharacterized protein LOC120090219 [Benincasa hispida])

HSP 1 Score: 104 bits (259), Expect = 1.98e-23
Identity = 66/167 (39.52%), Postives = 88/167 (52.69%), Query Frame = 0


Query: 1   KTNCPKRMNILIWILLNSSSINAMDVLKWKLSYSQLLPSVCFLCFADSENQNHLLFGCSL 60
           KT  P+R+NILIWI+L    +N  +VL+ K     L P+VC  C   SE   HL F C  
Sbjct: 149 KTKSPRRVNILIWIML-FGQLNVAEVLQKKQPTPSLSPTVCPFCLHHSEVSLHLFFTCPY 208

Query: 61  SLKCWFWVLKKFWILWAFSNDYKNNVLNLLVGPSLKSSS-----NKVKANSSELWLERNH 120
           S  CW  +L  F +     ND+K+NV  LL  P+   S+     N VKA  ++LW ERN 
Sbjct: 209 SSWCWNKLLCFFNLPLTLCNDFKSNVFQLLARPTSHKSTRLLWCNAVKALLADLWFERNQ 268

Query: 121 IIFQKKICCSLDQFDMALKSLTR--SLSNTFLDYNFVDISLNWEAFI 160
            IF  K     D+ + A +  +    LS+ F  Y+  D +LNWEAFI
Sbjct: 269 RIFYNKATSCQDRLEAARRQASSWCLLSDPFRAYSLSDFNLNWEAFI 314

[Close]

BLAST of MC11g1207.1 vs. NCBI nr
Match: XP_022153214.1 (uncharacterized protein LOC111020765 [Momordica charantia])

HSP 1 Score: 99.4 bits (246), Expect = 1.56e-22
Identity = 65/168 (38.69%), Postives = 87/168 (51.79%), Query Frame = 0


Query: 1   KTNCPKRMNILIWILLNSSSINAMDVLKWKLSYSQLLPSVCFLCFADSENQNHLLFGCSL 60
           KT  P+R+N+  WIL     +N  D+++ K     LLPS C LC    E+ +HL F C  
Sbjct: 34  KTKSPRRVNVFCWILFQGK-LNTADIIQKKSPSDALLPSFCCLCTKSGEDHDHLFFHCYF 93

Query: 61  SLKCWFWVLKKFWILWAFSNDYKNNVLNLLVGPSLKSSS------NKVKANSSELWLERN 120
           + KCW  +  +F + W F     +NV  LL GP   SSS      N VKA  SELW ERN
Sbjct: 94  ASKCWNLLFHQFNVDWCFDLKAGDNVYQLLHGPPHLSSSVRFLWLNVVKALLSELWFERN 153

Query: 121 HIIFQKKICCSLDQFDMAL--KSLTRSLSNTFLDYNFVDISLNWEAFI 160
             +F++K     + F  A    SL  SL ++FL ++   I  NW AFI
Sbjct: 154 SRLFEEKRRLFDESFYSAKFKASLWCSLVDSFLHHSPSMIYANWGAFI 200

[Close]

BLAST of MC11g1207.1 vs. NCBI nr
Match: KAA0062564.1 (GPI-anchor transamidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 88.6 bits (218), Expect = 5.54e-17
Identity = 54/158 (34.18%), Postives = 86/158 (54.43%), Query Frame = 0


Query: 11  LIWILLNSSSINAMD-VLKWKLSYSQLLPSVCFLCFADSENQNHLLFGCSLSLKCWFWVL 70
           + W L +S+ +     V++ +L  S L+PS C LC  + E+   L F C  S+KCW  +L
Sbjct: 542 IFWFLHHSNFVIVGSLVMQRELLNSCLMPSACSLCLEEGEDLQPL-FTCPYSVKCWENLL 601

Query: 71  KKFWILWAFSNDYKNNVLNLLVGPSLKSS-----SNKVKANSSELWLERNHIIFQKKICC 130
             F + WAF   + +N+  +L+G SLK        N  KA  S++W E N  IF+ K+  
Sbjct: 602 SLFGVDWAFDGSFNSNIKQILLGSSLKEGPRLIWGNMSKALLSDIWFECNQRIFRGKVMH 661

Query: 131 SLDQFDMALKSLTR--SLSNTFLDYNFVDISLNWEAFI 160
             ++ D+A ++      L+  F+DY+  D+S+NW AFI
Sbjct: 662 WSERLDIAKRNAATWCMLNKEFIDYSIQDLSVNWLAFI 698

[Close]

BLAST of MC11g1207.1 vs. NCBI nr
Match: XP_034223765.1 (THO complex subunit 1 isoform X5 [Prunus dulcis])

HSP 1 Score: 60.8 bits (146), Expect = 2.36e-07
Identity = 49/168 (29.17%), Postives = 74/168 (44.05%), Query Frame = 0


Query: 1   KTNCPKRMNILIWILLNSSSINAMDVLKWKLSYSQLLPSVCFLCFADSENQNHLLFGCSL 60
           K   P ++   +W+  N   IN  D ++ +     L PS C LC  ++EN +HL   CS 
Sbjct: 449 KAKTPPKIQFFVWLAANGR-INTCDCIQRRQPKMCLSPSWCVLCKENAENIDHLFIHCSY 508

Query: 61  SLKCWFWVLKKFWILWAFSND-YKNNVLNLLVGPSLKSSSNK------VKANSSELWLER 120
           SLK W+ +L      W      ++   +NL V    K           V A    +W+ER
Sbjct: 509 SLKLWWRMLGALRAEWVIPKGCFELLSINLRVSGKGKGKRAGILHDCLVHAIFGNIWMER 568

Query: 121 NHIIFQKKICCSLDQFDMALK---SLTRSLSNTFLDYNFVDISLNWEA 158
           N  IFQ  I   +++    +K   SL  S+S  F DY++  I  +  A
Sbjct: 569 NRRIFQGHIGVRVEELWDRIKFWASLWASVSGQFKDYHYSTIMRDMMA 615

[Close]

BLAST of MC11g1207.1 vs. NCBI nr
Match: XP_034223763.1 (THO complex subunit 1 isoform X4 [Prunus dulcis])

HSP 1 Score: 60.8 bits (146), Expect = 2.38e-07
Identity = 49/168 (29.17%), Postives = 74/168 (44.05%), Query Frame = 0


Query: 1   KTNCPKRMNILIWILLNSSSINAMDVLKWKLSYSQLLPSVCFLCFADSENQNHLLFGCSL 60
           K   P ++   +W+  N   IN  D ++ +     L PS C LC  ++EN +HL   CS 
Sbjct: 474 KAKTPPKIQFFVWLAANGR-INTCDCIQRRQPKMCLSPSWCVLCKENAENIDHLFIHCSY 533

Query: 61  SLKCWFWVLKKFWILWAFSND-YKNNVLNLLVGPSLKSSSNK------VKANSSELWLER 120
           SLK W+ +L      W      ++   +NL V    K           V A    +W+ER
Sbjct: 534 SLKLWWRMLGALRAEWVIPKGCFELLSINLRVSGKGKGKRAGILHDCLVHAIFGNIWMER 593

Query: 121 NHIIFQKKICCSLDQFDMALK---SLTRSLSNTFLDYNFVDISLNWEA 158
           N  IFQ  I   +++    +K   SL  S+S  F DY++  I  +  A
Sbjct: 594 NRRIFQGHIGVRVEELWDRIKFWASLWASVSGQFKDYHYSTIMRDMMA 640

[Close]

BLAST of MC11g1207.1 vs. ExPASy TrEMBL
Match: A0A6J1DIE2 (uncharacterized protein LOC111020765 OS=Momordica charantia OX=3673 GN=LOC111020765 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 7.56e-23
Identity = 65/168 (38.69%), Postives = 87/168 (51.79%), Query Frame = 0


Query: 1   KTNCPKRMNILIWILLNSSSINAMDVLKWKLSYSQLLPSVCFLCFADSENQNHLLFGCSL 60
           KT  P+R+N+  WIL     +N  D+++ K     LLPS C LC    E+ +HL F C  
Sbjct: 34  KTKSPRRVNVFCWILFQGK-LNTADIIQKKSPSDALLPSFCCLCTKSGEDHDHLFFHCYF 93

Query: 61  SLKCWFWVLKKFWILWAFSNDYKNNVLNLLVGPSLKSSS------NKVKANSSELWLERN 120
           + KCW  +  +F + W F     +NV  LL GP   SSS      N VKA  SELW ERN
Sbjct: 94  ASKCWNLLFHQFNVDWCFDLKAGDNVYQLLHGPPHLSSSVRFLWLNVVKALLSELWFERN 153

Query: 121 HIIFQKKICCSLDQFDMAL--KSLTRSLSNTFLDYNFVDISLNWEAFI 160
             +F++K     + F  A    SL  SL ++FL ++   I  NW AFI
Sbjct: 154 SRLFEEKRRLFDESFYSAKFKASLWCSLVDSFLHHSPSMIYANWGAFI 200

[Close]

BLAST of MC11g1207.1 vs. ExPASy TrEMBL
Match: A0A5A7V5N8 (GPI-anchor transamidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79G00520 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 2.68e-17
Identity = 54/158 (34.18%), Postives = 86/158 (54.43%), Query Frame = 0


Query: 11  LIWILLNSSSINAMD-VLKWKLSYSQLLPSVCFLCFADSENQNHLLFGCSLSLKCWFWVL 70
           + W L +S+ +     V++ +L  S L+PS C LC  + E+   L F C  S+KCW  +L
Sbjct: 542 IFWFLHHSNFVIVGSLVMQRELLNSCLMPSACSLCLEEGEDLQPL-FTCPYSVKCWENLL 601

Query: 71  KKFWILWAFSNDYKNNVLNLLVGPSLKSS-----SNKVKANSSELWLERNHIIFQKKICC 130
             F + WAF   + +N+  +L+G SLK        N  KA  S++W E N  IF+ K+  
Sbjct: 602 SLFGVDWAFDGSFNSNIKQILLGSSLKEGPRLIWGNMSKALLSDIWFECNQRIFRGKVMH 661

Query: 131 SLDQFDMALKSLTR--SLSNTFLDYNFVDISLNWEAFI 160
             ++ D+A ++      L+  F+DY+  D+S+NW AFI
Sbjct: 662 WSERLDIAKRNAATWCMLNKEFIDYSIQDLSVNWLAFI 698

[Close]

BLAST of MC11g1207.1 vs. ExPASy TrEMBL
Match: A0A5A7T2Y0 (zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold403G00100 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.16e-13
Identity = 52/165 (31.52%), Postives = 84/165 (50.91%), Query Frame = 0


Query: 1   KTNCPKRMNILIWILLNSSSINAMDVLKWKLSYSQLLPSVCFLCFADSENQNHLLFGCSL 60
           ++  P+R+NILIWI++    I++ ++L+ K S   + PS+C LC   S+N  H+   C +
Sbjct: 219 QSGSPRRINILIWIMVFRFVISS-EILQKK-SPINVSPSICPLCLKASKNLPHIFLYCPV 278

Query: 61  SLKCWFWVLKKFWILWAFSNDYKNNVLNLLVGPSLKSSSNKV-----KANSSELWLERNH 120
           S   W  +   F ++W F +    +V+ LL G +L  +   +     KA   E+W+ERN 
Sbjct: 279 SSFGWERIFSLFNLVWNFDSSLSASVIQLLSGSNLPKTPRIIWEILSKALLIEIWIERNQ 338

Query: 121 IIFQKKICCSLDQFDMALKSLTR--SLSNTFLDYNFVDISLNWEA 158
            IF  K     +    A  +     SL   F++Y+  DI LNW A
Sbjct: 339 RIFHDKARQRAEIMHAADLNAAAWCSLRKEFVNYSIQDICLNWNA 381

[Close]

BLAST of MC11g1207.1 vs. ExPASy TrEMBL
Match: M5XV38 (zf-RVT domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_ppa019854mg PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.08e-07
Identity = 47/166 (28.31%), Postives = 76/166 (45.78%), Query Frame = 0


Query: 1   KTNCPKRMNILIWILLNSSSINAMDVLKWKLSYSQLLPSVCFLCFADSENQNHLLFGCSL 60
           K   P ++   +W+  N   IN  D ++ +     L PS C LC  ++EN +HL   CS 
Sbjct: 302 KAKTPPKIQFFVWLAANGR-INTCDCIQRRQPKMCLSPSWCVLCKENAENIDHLFIHCSY 361

Query: 61  SLKCWFWVLKKFWILWAFSND-YKNNVLNLLVGPSLKSSSNK----VKANSSELWLERNH 120
           SL+ W+ +L    + W      ++   +NL +    K +       V A    +W+ERN 
Sbjct: 362 SLRLWWKMLGALGVEWVIPKGCFELLSINLRISGKGKRAGILRDCLVHAIFWNIWMERNQ 421

Query: 121 IIFQKKICCSLDQFDMALK---SLTRSLSNTFLDYNFVDISLNWEA 158
            IFQ  I   +++    +K   SL  S+S  F DY++  I  +  A
Sbjct: 422 RIFQGHIGVRVEELWDRIKFWASLWASVSGQFKDYHYSTIMRDMMA 466

[Close]

BLAST of MC11g1207.1 vs. ExPASy TrEMBL
Match: M5WJ76 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa015871mg PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.27e-07
Identity = 47/166 (28.31%), Postives = 76/166 (45.78%), Query Frame = 0


Query: 1    KTNCPKRMNILIWILLNSSSINAMDVLKWKLSYSQLLPSVCFLCFADSENQNHLLFGCSL 60
            K   P ++   +W+  N   IN  D ++ +     L PS C LC  ++EN +HL   CS 
Sbjct: 1332 KAKTPPKIQFFVWLAANGR-INTCDCIQRRQPKMCLSPSWCVLCKENAENIDHLFIHCSY 1391

Query: 61   SLKCWFWVLKKFWILWAFSND-YKNNVLNLLVGPSLKSSSNK----VKANSSELWLERNH 120
            SL+ W+ +L    + W      ++   +NL +    K +       V A    +W+ERN 
Sbjct: 1392 SLRLWWKMLGALGVEWVIPKGCFELLSINLRISGKGKRAGILRDCLVHAIFWNIWMERNQ 1451

Query: 121  IIFQKKICCSLDQFDMALK---SLTRSLSNTFLDYNFVDISLNWEA 158
             IFQ  I   +++    +K   SL  S+S  F DY++  I  +  A
Sbjct: 1452 RIFQGHIGVRVEELWDRIKFWASLWASVSGQFKDYHYSTIMRDMMA 1496

The following BLAST results are available for this feature:

BLAST of MC11g1207.1 vs.


Match Name	E-value	Identity	Description

BLAST of MC11g1207.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903695.1	1.98e-23	39.52	uncharacterized protein LOC120090219 [Benincasa hispida]	[more]
XP_022153214.1	1.56e-22	38.69	uncharacterized protein LOC111020765 [Momordica charantia]	[more]
KAA0062564.1	5.54e-17	34.18	GPI-anchor transamidase isoform X1 [Cucumis melo var. makuwa]	[more]
XP_034223765.1	2.36e-07	29.17	THO complex subunit 1 isoform X5 [Prunus dulcis]	[more]
XP_034223763.1	2.38e-07	29.17	THO complex subunit 1 isoform X4 [Prunus dulcis]	[more]

BLAST of MC11g1207.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIE2	7.56e-23	38.69	uncharacterized protein LOC111020765 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A5A7V5N8	2.68e-17	34.18	GPI-anchor transamidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A5A7T2Y0	6.16e-13	31.52	zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
M5XV38	1.08e-07	28.31	zf-RVT domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_ppa019854mg ...	[more]
M5WJ76	1.27e-07	28.31	Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=...	[more]

BLAST of MC11g1207.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026960	Reverse transcriptase zinc-binding domain	PFAM	PF13966	zf-RVT	coord: 1..65 e-value: 3.7E-7 score: 30.7

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC11g1207	MC11g1207	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g1207.1-cds	MC11g1207.1-cds-MC11:11773868..11774152	CDS
MC11g1207.1-cds	MC11g1207.1-cds-MC11:11774158..11774268	CDS
MC11g1207.1-cds	MC11g1207.1-cds-MC11:11774271..11774285	CDS
MC11g1207.1-cds	MC11g1207.1-cds-MC11:11774290..11774358	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC11g1207.1	MC11g1207.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity