MC11g1002.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC11g1002.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	indole-3-acetate O-methyltransferase 1-like
Location	MC11: 8588184 .. 8589399 (-)
Sequence length	1008
RNA-Seq Expression	MC11g1002.1
Synteny	MC11g1002.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCCGTCATGCAAGAGCAATGCTTCACCTTCTCAAAGAAACCCTCGACATCGTCCATCTGAACTCGCCGGAGCTGCCATTTACAGTGGCGGACCTCGGCTGCTCCTCCGGGAGGAACACCATCACCACCGTAGATTTCATCGTCGACCACATAACCAATCGCTACAAGGCTTTGGGTAATCGACTGCCGGAGTTTTCTGCCATCTTTTCCGACCTCCCCAGTAATGACTTCAACACCCTTTTCCAGCTCCTCCATCCGGCTGCTGGGAACCGCCGGTCCTACTTCGCCGCTGGCGTACCTGGGTCGTTCTACCACCGACTTTTTCCGGCAAGATCCATCAATGTTTTTCACTCTGTTTTCTCCTTGCATTGGCTCTCTCAGGTGCCGGAAAGTGTGGTGGACGGCGGATCGGTGGCGTACAACAAAGGAAAGGTTTTCATTCACCGGGGAAATAAGGCGGCGGCCGCCGCCTACAGACGGCAGTTTCAGGCGGACCTGGCCGGATTCCTCCAGTCAAGATCGGAGGAGCTGAAGAAGGGTGGGGCCATGTTTGTTGCTTGCTTGGGAAGAACCTCTGTGGACCCCACCGACCAAGGTGGGGCCGGAATCCTCTTTGGGACCCACTTTGAGGATGCTTGGGAAGATCTTGTCCAAGAGGTATATAAGTATATATATATTATAAAATTTCTCCATTTTTCAATGAATGTCATATGAAAATTTAAGGGTAAGAATTAAGATTAGAGTTTTTATGGAGAGTTTATAGGAAAAACAAAACATTTGAAAATATTAAGGTAAAAGAAAAGCATTAAATTCTAAAATATATAATAATGAGAGCTTGTTTGGTTATATTTTTGTGGTAATGTAGGGATGGATAACTAGTGAGAAACGGGATAGTTTCAACATTCCACTATATGCACCAAGCTTACAAGATTTTAAGGAAGTGATCGAAGCAAATAGTTCATTTTCCATAAACATGCTTGAGGTTTTCAAAGGAGGCAGCCCATTGGTGGTTAACCAGCCCGATGATGCAACTGAAGTCGGTCGAGCTATGGCCAACACATGTCGGAGTGTTTCGAGCGCCCTTGTCGATGCTCATATCGGGGATCGCCTTAGCAAGAAGCTCTTTACTCGAGTTGAACATAGAGCCACATGCCATGCCAAAGACCTATTTGAGAAACTCCAATTCTTTCACATTGTTGCATCACTTTCCCTTGCT

mRNA sequence

GCCCGTCATGCAAGAGCAATGCTTCACCTTCTCAAAGAAACCCTCGACATCGTCCATCTGAACTCGCCGGAGCTGCCATTTACAGTGGCGGACCTCGGCTGCTCCTCCGGGAGGAACACCATCACCACCGTAGATTTCATCGTCGACCACATAACCAATCGCTACAAGGCTTTGGGTAATCGACTGCCGGAGTTTTCTGCCATCTTTTCCGACCTCCCCAGTAATGACTTCAACACCCTTTTCCAGCTCCTCCATCCGGCTGCTGGGAACCGCCGGTCCTACTTCGCCGCTGGCGTACCTGGGTCGTTCTACCACCGACTTTTTCCGGCAAGATCCATCAATGTTTTTCACTCTGTTTTCTCCTTGCATTGGCTCTCTCAGGTGCCGGAAAGTGTGGTGGACGGCGGATCGGTGGCGTACAACAAAGGAAAGGTTTTCATTCACCGGGGAAATAAGGCGGCGGCCGCCGCCTACAGACGGCAGTTTCAGGCGGACCTGGCCGGATTCCTCCAGTCAAGATCGGAGGAGCTGAAGAAGGGTGGGGCCATGTTTGTTGCTTGCTTGGGAAGAACCTCTGTGGACCCCACCGACCAAGGTGGGGCCGGAATCCTCTTTGGGACCCACTTTGAGGATGCTTGGGAAGATCTTGTCCAAGAGGGATGGATAACTAGTGAGAAACGGGATAGTTTCAACATTCCACTATATGCACCAAGCTTACAAGATTTTAAGGAAGTGATCGAAGCAAATAGTTCATTTTCCATAAACATGCTTGAGGTTTTCAAAGGAGGCAGCCCATTGGTGGTTAACCAGCCCGATGATGCAACTGAAGTCGGTCGAGCTATGGCCAACACATGTCGGAGTGTTTCGAGCGCCCTTGTCGATGCTCATATCGGGGATCGCCTTAGCAAGAAGCTCTTTACTCGAGTTGAACATAGAGCCACATGCCATGCCAAAGACCTATTTGAGAAACTCCAATTCTTTCACATTGTTGCATCACTTTCCCTTGCT

Coding sequence (CDS)

GCCCGTCATGCAAGAGCAATGCTTCACCTTCTCAAAGAAACCCTCGACATCGTCCATCTGAACTCGCCGGAGCTGCCATTTACAGTGGCGGACCTCGGCTGCTCCTCCGGGAGGAACACCATCACCACCGTAGATTTCATCGTCGACCACATAACCAATCGCTACAAGGCTTTGGGTAATCGACTGCCGGAGTTTTCTGCCATCTTTTCCGACCTCCCCAGTAATGACTTCAACACCCTTTTCCAGCTCCTCCATCCGGCTGCTGGGAACCGCCGGTCCTACTTCGCCGCTGGCGTACCTGGGTCGTTCTACCACCGACTTTTTCCGGCAAGATCCATCAATGTTTTTCACTCTGTTTTCTCCTTGCATTGGCTCTCTCAGGTGCCGGAAAGTGTGGTGGACGGCGGATCGGTGGCGTACAACAAAGGAAAGGTTTTCATTCACCGGGGAAATAAGGCGGCGGCCGCCGCCTACAGACGGCAGTTTCAGGCGGACCTGGCCGGATTCCTCCAGTCAAGATCGGAGGAGCTGAAGAAGGGTGGGGCCATGTTTGTTGCTTGCTTGGGAAGAACCTCTGTGGACCCCACCGACCAAGGTGGGGCCGGAATCCTCTTTGGGACCCACTTTGAGGATGCTTGGGAAGATCTTGTCCAAGAGGGATGGATAACTAGTGAGAAACGGGATAGTTTCAACATTCCACTATATGCACCAAGCTTACAAGATTTTAAGGAAGTGATCGAAGCAAATAGTTCATTTTCCATAAACATGCTTGAGGTTTTCAAAGGAGGCAGCCCATTGGTGGTTAACCAGCCCGATGATGCAACTGAAGTCGGTCGAGCTATGGCCAACACATGTCGGAGTGTTTCGAGCGCCCTTGTCGATGCTCATATCGGGGATCGCCTTAGCAAGAAGCTCTTTACTCGAGTTGAACATAGAGCCACATGCCATGCCAAAGACCTATTTGAGAAACTCCAATTCTTTCACATTGTTGCATCACTTTCCCTTGCT

Protein sequence

ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGNRLPEFSAIFSDLPSNDFNTLFQLLHPAAGNRRSYFAAGVPGSFYHRLFPARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAGFLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRDSFNIPLYAPSLQDFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSVSSALVDAHIGDRLSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA

Homology

[Close]

BLAST of MC11g1002.1 vs. ExPASy Swiss-Prot
Match: Q9FLN8 (Indole-3-acetate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=IAMT1 PE=1 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 1.7e-134
Identity = 237/347 (68.30%), Postives = 278/347 (80.12%), Query Frame = 0


Query: 1   ARHARAMLHLLKETLDIVHLNSPEL--PFTVADLGCSSGRNTITTVDFIVDHITNRYKAL 60
           A HAR+MLHLL+ETL+ VHLNS     PFT  DLGCSSG NT+  +DFIV HI+ R+ A 
Sbjct: 38  AMHARSMLHLLEETLENVHLNSSASPPPFTAVDLGCSSGANTVHIIDFIVKHISKRFDAA 97

Query: 61  GNRLPEFSAIFSDLPSNDFNTLFQLLHP-----------AAGNRRSYFAAGVPGSFYHRL 120
           G   PEF+A FSDLPSNDFNTLFQLL P           AA   RSYF AGVPGSFY RL
Sbjct: 98  GIDPPEFTAFFSDLPSNDFNTLFQLLPPLVSNTCMEECLAADGNRSYFVAGVPGSFYRRL 157

Query: 121 FPARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLA 180
           FPAR+I+ FHS FSLHWLSQVPESV D  S AYN+G+VFIH   +    AY+RQFQADLA
Sbjct: 158 FPARTIDFFHSAFSLHWLSQVPESVTDRRSAAYNRGRVFIHGAGEKTTTAYKRQFQADLA 217

Query: 181 GFLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKR 240
            FL++R+ E+K+GGAMF+ CLGRTSVDPTDQGGAG+LFGTHF+DAW+DLV+EG + +EKR
Sbjct: 218 EFLRARAAEVKRGGAMFLVCLGRTSVDPTDQGGAGLLFGTHFQDAWDDLVREGLVAAEKR 277

Query: 241 DSFNIPLYAPSLQDFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRS 300
           D FNIP+YAPSLQDFKEV++AN SF+I+ L V+KGGSPLVVN+PDDA+EVGRA A++CRS
Sbjct: 278 DGFNIPVYAPSLQDFKEVVDANGSFAIDKLVVYKGGSPLVVNEPDDASEVGRAFASSCRS 337

Query: 301 VSSALVDAHIGDRLSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLS 335
           V+  LV+AHIG+ LS KLF+RVE RAT HAKD+   LQFFHIVASLS
Sbjct: 338 VAGVLVEAHIGEELSNKLFSRVESRATSHAKDVLVNLQFFHIVASLS 384

[Close]

BLAST of MC11g1002.1 vs. ExPASy Swiss-Prot
Match: Q0J998 (Indole-3-acetate O-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=IAMT1 PE=1 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 1.9e-120
Identity = 216/355 (60.85%), Postives = 267/355 (75.21%), Query Frame = 0


Query: 1   ARHARAMLHLLKETLD-IVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALG 60
           A HAR MLH L+ETLD ++  +S +  FT ADLGCS G N++  VD IV  ++  Y++ G
Sbjct: 49  ALHARRMLHFLEETLDAMMERSSSDKLFTAADLGCSCGSNSLFIVDVIVRRVSEAYESRG 108

Query: 61  NRLPEFSAIFSDLPSNDFNTLFQLLHP------------------AAGNRRSYFAAGVPG 120
              PEF   FSDLPSNDFNTLFQLL P                  AA   R Y AAGVPG
Sbjct: 109 RDAPEFQVFFSDLPSNDFNTLFQLLPPLLAPVAGSLEECLAAGEGAATATRPYHAAGVPG 168

Query: 121 SFYHRLFPARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQ 180
           +FY RLFP  SI+VF S FSLHWLSQVPE V D  S AYN G+VF+HR  +A AAAY+RQ
Sbjct: 169 TFYGRLFPGESIDVFTSTFSLHWLSQVPEEVGDSASPAYNGGRVFVHRATEAVAAAYKRQ 228

Query: 181 FQADLAGFLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGW 240
           FQADLA FL+SR+ E+K+GGAMF+ACLGR+S DP DQGGAG+LFGTHF+DAW+DLVQEG 
Sbjct: 229 FQADLARFLRSRAREMKRGGAMFLACLGRSSGDPADQGGAGLLFGTHFQDAWDDLVQEGV 288

Query: 241 ITSEKRDSFNIPLYAPSLQDFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAM 300
           +  EKRDSFNIP+YAPSLQ+F++V+ A+ +F+I+ LE+ +GGSPLVV++PDDA EVGRAM
Sbjct: 289 VEGEKRDSFNIPVYAPSLQEFRDVVRADGAFAIDRLELVRGGSPLVVDRPDDAAEVGRAM 348

Query: 301 ANTCRSVSSALVDAHIGDRLSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA 337
           AN+C++V+  LVDAHIG+R   +LF R+E RA  HA++L EK+ FFH+V SLSLA
Sbjct: 349 ANSCKAVAGVLVDAHIGERRGAQLFERLERRAARHARELVEKMHFFHVVCSLSLA 403

[Close]

BLAST of MC11g1002.1 vs. ExPASy Swiss-Prot
Match: Q5XF78 (Gibberellic acid methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GAMT2 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.9e-56
Identity = 131/327 (40.06%), Postives = 175/327 (53.52%), Query Frame = 0


Query: 10  LLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKAL--GNRLPEFSA 69
           +L  +L  + L S + P  + DLGC++G NT TTVD +V+ +  RY A   G   PEF A
Sbjct: 50  ILISSLQSIKLFSDQTPIKITDLGCATGSNTFTTVDTVVETLQRRYTARCGGGGSPEFEA 109

Query: 70  IFSDLPSNDFNTLFQLL--HPAAGNRRSYFAAGVPGSFYHRLFPARSINVFHSVFSLHWL 129
            F DLPSNDFN LF+LL       +   YFA GV GSFY RLFP  +I+V  S+ +LHWL
Sbjct: 110 FFCDLPSNDFNMLFKLLAEKQKVDSPAKYFAGGVAGSFYDRLFPRGTIHVAVSLSALHWL 169

Query: 130 SQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAGFLQSRSEELKKGGAMFV 189
           SQ+PE V++  S  +NKGK +I    K    AY  Q   DL  F+  R EE+ KGG +FV
Sbjct: 170 SQIPEKVLEKESRTWNKGKTWIEGAKKEVVEAYAEQSDKDLDDFMSCRKEEMVKGGVLFV 229

Query: 190 ACLGRTSVDPTDQGG----AGILFGTHFEDAWEDLVQEGWITSEKRDSFNIPLYAPSLQD 249
              GR S   +  G     A   F T  E AW+DL++EG I  E RD FNIP Y  S ++
Sbjct: 230 LMAGRPSGSSSQFGDQDTRAKHPFTTTMEQAWQDLIEEGLIDEETRDGFNIPAYMRSPEE 289

Query: 250 FKEVIEANSSFSINMLEVFKGGSPLVVNQPD--------DATEVGRAMANTCRSVSSALV 309
               I+    F I  ++  K     +V   D        D    GRA  N  ++    +V
Sbjct: 290 VTAGIDRCGGFKIGKMDFLK-----IVEYSDEKQEEWKKDPVSYGRARTNLVQAAIRPMV 349

Query: 310 DAHIGDRLSKKLFTRVEHRATCHAKDL 321
           DA++G  LS +LF R E+R + + + L
Sbjct: 350 DAYLGPDLSHELFKRYENRVSTNQEFL 371

[Close]

BLAST of MC11g1002.1 vs. ExPASy Swiss-Prot
Match: A0A061DFA8 (Probable methyltransferase TCM_000168 OS=Theobroma cacao OX=3641 GN=TCM_000168 PE=3 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 3.0e-49
Identity = 122/321 (38.01%), Postives = 167/321 (52.02%), Query Frame = 0


Query: 4   ARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGNRLP 63
           +R+M  L +  LD    N PE   T+ADLGCSSG NT   V  I   I  R   LG   P
Sbjct: 28  SRSMPVLKQAVLDSCCTNLPE-TVTMADLGCSSGPNTFYAVSEITSIIYKRCCQLGRSAP 87

Query: 64  EFSAIFSDLPSNDFNTLFQLLHPAAGNRRS--------YFAAGVPGSFYHRLFPARSINV 123
           EF    +DLP NDFNT+FQ L    G  R          + AGVPGSFY RLFP ++++ 
Sbjct: 88  EFRVFLNDLPGNDFNTVFQSLPAYQGKLREENGPGFGPLYVAGVPGSFYGRLFPLKTLHF 147

Query: 124 FHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGN-KAAAAAYRRQFQADLAGFLQSRS 183
            HS  SLHWLSQVP  + D  +   NK K+FI + +  A  ++Y  QF+ D + FL+ RS
Sbjct: 148 VHSSSSLHWLSQVPPELTDKANPLINKWKIFISKTSPPAVISSYLTQFRKDFSLFLKLRS 207

Query: 184 EELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRDSFNIPL 243
           EE+   G M +   GRT+ DPT       L   +   A++DLV EG +  E+ D++N P 
Sbjct: 208 EEVAPEGRMVLTFRGRTTADPTSD--ESCLLWDYLGQAFQDLVAEGLVEEERLDTYNTPY 267

Query: 244 YAPSLQDFKEVIEANSSFSINMLEVF-------KGGSPLVVNQPDDATEVGRAMANTCRS 303
           Y P  ++ +  IE   SF+++ LEV         GG        DD     + MA   R+
Sbjct: 268 YEPHPEEIQAEIEKEGSFTLDRLEVIALPWDSVNGGI------KDDRATTAKRMAKAIRA 327

Query: 304 VSSALVDAHIGDRLSKKLFTR 309
           V+ ++  +H G+ +   LF R
Sbjct: 328 VNESMFRSHFGEDILDPLFHR 339

[Close]

BLAST of MC11g1002.1 vs. ExPASy Swiss-Prot
Match: F4JUY5 (Gibberellic acid methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=GAMT1 PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 8.6e-49
Identity = 123/317 (38.80%), Postives = 168/317 (53.00%), Query Frame = 0


Query: 29  VADLGCSSGRNTITTVDFIVDHITNRYKAL-GNRLP--EFSAIFSDLPSNDFNTLFQLL- 88
           +ADLGC+ G NT +TV+ +V+ +  +   + G   P  EF   FSDL SNDFN LF+ L 
Sbjct: 59  IADLGCAIGDNTFSTVETVVEVLGKKLAVIDGGTEPEMEFEVFFSDLSSNDFNALFRSLD 118

Query: 89  HPAAGNRRSYFAAGVPGSFYHRLFPARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGK 148
               G+ R YFAAGVPGSFY RLFP   ++V  ++ +L WLSQVPE V++ GS ++NKG 
Sbjct: 119 EKVNGSSRKYFAAGVPGSFYKRLFPKGELHVVVTMSALQWLSQVPEKVMEKGSKSWNKGG 178

Query: 149 VFIHRGNKAAAAAYRRQFQADLAGFLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGIL 208
           V+I    K    AY  Q   DL  FL+ R EE+  GG +F+   GR S      G     
Sbjct: 179 VWIEGAEKEVVEAYAEQADKDLVEFLKCRKEEIVVGGVLFMLMGGRPSGSVNQIGDPDSS 238

Query: 209 ----FGTHFEDAWEDLVQEGWITSEKRDSFNIPLYAPSLQDFKEVIEANSSFSINMLE-- 268
               F T  + AW+DLV EG I  EKRD FNIP+Y  + ++    I+    F I   E  
Sbjct: 239 LKHPFTTLMDQAWQDLVDEGLIEEEKRDGFNIPVYFRTTEEIAAAIDRCGGFKIEKTENL 298

Query: 269 ----VFKGGSPLVVNQPDDATEVGRAMANTCRSVSSALVDAHIGDRLSKKLFTRVEHRAT 328
                  G    ++  PD     GR  AN  ++    +V A++G  L+ KLF R   RA 
Sbjct: 299 IIADHMNGKQEELMKDPD---SYGRDRANYAQAGLKPIVQAYLGPDLTHKLFKRYAVRAA 358

Query: 329 CHAKDLFEKLQFFHIVA 332
              K++     F+H++A
Sbjct: 359 AD-KEILNNC-FYHMIA 370

[Close]

BLAST of MC11g1002.1 vs. NCBI nr
Match: XP_022139646.1 (indole-3-acetate O-methyltransferase 1-like [Momordica charantia])

HSP 1 Score: 670 bits (1729), Expect = 2.66e-242
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0


Query: 1   ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN 60
           ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN
Sbjct: 39  ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN 98

Query: 61  RLPEFSAIFSDLPSNDFNTLFQLLHPAAGNRRSYFAAGVPGSFYHRLFPARSINVFHSVF 120
           RLPEFSAIFSDLPSNDFNTLFQLLHPAAGNRRSYFAAGVPGSFYHRLFPARSINVFHSVF
Sbjct: 99  RLPEFSAIFSDLPSNDFNTLFQLLHPAAGNRRSYFAAGVPGSFYHRLFPARSINVFHSVF 158

Query: 121 SLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAGFLQSRSEELKKG 180
           SLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAGFLQSRSEELKKG
Sbjct: 159 SLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAGFLQSRSEELKKG 218

Query: 181 GAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRDSFNIPLYAPSLQ 240
           GAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRDSFNIPLYAPSLQ
Sbjct: 219 GAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRDSFNIPLYAPSLQ 278

Query: 241 DFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSVSSALVDAHIGDR 300
           DFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSVSSALVDAHIGDR
Sbjct: 279 DFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSVSSALVDAHIGDR 338

Query: 301 LSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA 336
           LSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA
Sbjct: 339 LSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA 374

[Close]

BLAST of MC11g1002.1 vs. NCBI nr
Match: KAG7024938.1 (Indole-3-acetate O-methyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 524 bits (1349), Expect = 2.97e-184
Identity = 266/348 (76.44%), Postives = 295/348 (84.77%), Query Frame = 0


Query: 1   ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN 60
           A+HAR++LHLLKETLD VHLNSPELPF V DLGCS G NTI  VD I+ HI  R++AL  
Sbjct: 38  AQHARSVLHLLKETLDGVHLNSPELPFVVVDLGCSCGSNTIYIVDVIIKHIVKRFEALAV 97

Query: 61  RLPEFSAIFSDLPSNDFNTLFQLLHP------------AAGNRRSYFAAGVPGSFYHRLF 120
             PEFSA FSDLP NDFNTLFQLL P            AA N RSYFAAGVPGSFY RLF
Sbjct: 98  DPPEFSAFFSDLPGNDFNTLFQLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLF 157

Query: 121 PARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAG 180
           PARSI+VFHS FSLHWLSQVPESVVD  S AYN+G+V IH  N+AAAAAYR+QFQ DLAG
Sbjct: 158 PARSIDVFHSAFSLHWLSQVPESVVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAG 217

Query: 181 FLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRD 240
           FL +R+ ELK+GG+MF+ CLGRTS+DPTDQGGAG+LFGTHF+DAW+DLVQEG I+SEKRD
Sbjct: 218 FLWARAHELKRGGSMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRD 277

Query: 241 SFNIPLYAPSLQDFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSV 300
           SFNIP+YAPSLQDFKEV+EA+ SFSIN LEVFKGGSPLVVNQPDDA EVGRA+AN+CRSV
Sbjct: 278 SFNIPVYAPSLQDFKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSV 337

Query: 301 SSALVDAHIGDRLSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA 336
           S  LVDAHIGDRLS++LF+RVE RAT HAKDL EKLQFFHIVASLSLA
Sbjct: 338 SGVLVDAHIGDRLSEELFSRVERRATNHAKDLLEKLQFFHIVASLSLA 385

[Close]

BLAST of MC11g1002.1 vs. NCBI nr
Match: KAG6592061.1 (Indole-3-acetate O-methyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 523 bits (1348), Expect = 4.22e-184
Identity = 265/348 (76.15%), Postives = 295/348 (84.77%), Query Frame = 0


Query: 1   ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN 60
           A+HAR++LHLLKETLD VHLNSPELPF V DLGCS G NTI  +D I+ HI  R++AL  
Sbjct: 38  AQHARSVLHLLKETLDGVHLNSPELPFVVVDLGCSCGSNTIYIIDVIIKHIVKRFEALAV 97

Query: 61  RLPEFSAIFSDLPSNDFNTLFQLLHP------------AAGNRRSYFAAGVPGSFYHRLF 120
             PEFSA FSDLP NDFNTLFQLL P            AA N RSYFAAGVPGSFY RLF
Sbjct: 98  DPPEFSAFFSDLPGNDFNTLFQLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLF 157

Query: 121 PARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAG 180
           PARSI+VFHS FSLHWLSQVPESVVD  S AYN+G+V IH  N+AAAAAYR+QFQ DLAG
Sbjct: 158 PARSIDVFHSAFSLHWLSQVPESVVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAG 217

Query: 181 FLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRD 240
           FL +R+ ELK+GG+MF+ CLGRTS+DPTDQGGAG+LFGTHF+DAW+DLVQEG I+SEKRD
Sbjct: 218 FLWARAHELKRGGSMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRD 277

Query: 241 SFNIPLYAPSLQDFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSV 300
           SFNIP+YAPSLQDFKEV+EA+ SFSIN LEVFKGGSPLVVNQPDDA EVGRA+AN+CRSV
Sbjct: 278 SFNIPVYAPSLQDFKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSV 337

Query: 301 SSALVDAHIGDRLSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA 336
           S  LVDAHIGDRLS++LF+RVE RAT HAKDL EKLQFFHIVASLSLA
Sbjct: 338 SGVLVDAHIGDRLSEELFSRVERRATNHAKDLLEKLQFFHIVASLSLA 385

[Close]

BLAST of MC11g1002.1 vs. NCBI nr
Match: XP_023536087.1 (indole-3-acetate O-methyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 523 bits (1347), Expect = 5.99e-184
Identity = 265/348 (76.15%), Postives = 295/348 (84.77%), Query Frame = 0


Query: 1   ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN 60
           A+HAR++LHLLKETLD VHLNSPELPF V DLGCS G NTI  +D I+ HI  R++AL  
Sbjct: 38  AQHARSVLHLLKETLDGVHLNSPELPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAV 97

Query: 61  RLPEFSAIFSDLPSNDFNTLFQLLHP------------AAGNRRSYFAAGVPGSFYHRLF 120
             PEFSA FSDLP NDFNTLFQLL P            AA N RSYFAAGVPGSFY RLF
Sbjct: 98  DPPEFSAFFSDLPGNDFNTLFQLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLF 157

Query: 121 PARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAG 180
           PARSI+VFHS FSLHWLSQVPESVVD  S AYN+G+V IH  N+AAAAAYR+QFQ DLAG
Sbjct: 158 PARSIDVFHSAFSLHWLSQVPESVVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAG 217

Query: 181 FLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRD 240
           FL +R+ ELK+GG+MF+ CLGRTS+DPTDQGGAG+LFGTHF+DAW+DLVQEG I+SEKRD
Sbjct: 218 FLWARAHELKRGGSMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRD 277

Query: 241 SFNIPLYAPSLQDFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSV 300
           SFNIP+YAPSLQDFKEV+EA+ SFSIN LEVFKGGSPLVVNQPDDA EVGRA+AN+CRSV
Sbjct: 278 SFNIPVYAPSLQDFKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSV 337

Query: 301 SSALVDAHIGDRLSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA 336
           S  LVDAHIGDRLS++LF+RVE RAT HAKDL EKLQFFHIVASLSLA
Sbjct: 338 SGVLVDAHIGDRLSEELFSRVERRATNHAKDLLEKLQFFHIVASLSLA 385

[Close]

BLAST of MC11g1002.1 vs. NCBI nr
Match: XP_022936266.1 (indole-3-acetate O-methyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 523 bits (1347), Expect = 5.99e-184
Identity = 265/348 (76.15%), Postives = 295/348 (84.77%), Query Frame = 0


Query: 1   ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN 60
           A+HAR++LHLLKETLD VHLNSPELPF V DLGCS G NTI  +D I+ HI  R++AL  
Sbjct: 38  AQHARSVLHLLKETLDGVHLNSPELPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAV 97

Query: 61  RLPEFSAIFSDLPSNDFNTLFQLLHP------------AAGNRRSYFAAGVPGSFYHRLF 120
             PEFSA FSDLP NDFNTLFQLL P            AA N RSYFAAGVPGSFY RLF
Sbjct: 98  DPPEFSAFFSDLPGNDFNTLFQLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLF 157

Query: 121 PARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAG 180
           PARSI+VFHS FSLHWLSQVPESVVD  S AYN+G+V IH  N+AAAAAYR+QFQ DLAG
Sbjct: 158 PARSIDVFHSAFSLHWLSQVPESVVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAG 217

Query: 181 FLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRD 240
           FL +R+ ELK+GG+MF+ CLGRTS+DPTDQGGAG+LFGTHF+DAW+DLVQEG I+SEKRD
Sbjct: 218 FLWARAHELKRGGSMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRD 277

Query: 241 SFNIPLYAPSLQDFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSV 300
           SFNIP+YAPSLQDFKEV+EA+ SFSIN LEVFKGGSPLVVNQPDDA EVGRA+AN+CRSV
Sbjct: 278 SFNIPVYAPSLQDFKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSV 337

Query: 301 SSALVDAHIGDRLSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA 336
           S  LVDAHIGDRLS++LF+RVE RAT HAKDL EKLQFFHIVASLSLA
Sbjct: 338 SGVLVDAHIGDRLSEELFSRVERRATNHAKDLLEKLQFFHIVASLSLA 385

[Close]

BLAST of MC11g1002.1 vs. ExPASy TrEMBL
Match: A0A6J1CDB0 (indole-3-acetate O-methyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111010498 PE=4 SV=1)

HSP 1 Score: 670 bits (1729), Expect = 1.29e-242
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0


Query: 1   ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN 60
           ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN
Sbjct: 39  ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN 98

Query: 61  RLPEFSAIFSDLPSNDFNTLFQLLHPAAGNRRSYFAAGVPGSFYHRLFPARSINVFHSVF 120
           RLPEFSAIFSDLPSNDFNTLFQLLHPAAGNRRSYFAAGVPGSFYHRLFPARSINVFHSVF
Sbjct: 99  RLPEFSAIFSDLPSNDFNTLFQLLHPAAGNRRSYFAAGVPGSFYHRLFPARSINVFHSVF 158

Query: 121 SLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAGFLQSRSEELKKG 180
           SLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAGFLQSRSEELKKG
Sbjct: 159 SLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAGFLQSRSEELKKG 218

Query: 181 GAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRDSFNIPLYAPSLQ 240
           GAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRDSFNIPLYAPSLQ
Sbjct: 219 GAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRDSFNIPLYAPSLQ 278

Query: 241 DFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSVSSALVDAHIGDR 300
           DFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSVSSALVDAHIGDR
Sbjct: 279 DFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSVSSALVDAHIGDR 338

Query: 301 LSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA 336
           LSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA
Sbjct: 339 LSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA 374

[Close]

BLAST of MC11g1002.1 vs. ExPASy TrEMBL
Match: A0A6J1F711 (indole-3-acetate O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442937 PE=4 SV=1)

HSP 1 Score: 523 bits (1347), Expect = 2.90e-184
Identity = 265/348 (76.15%), Postives = 295/348 (84.77%), Query Frame = 0


Query: 1   ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN 60
           A+HAR++LHLLKETLD VHLNSPELPF V DLGCS G NTI  +D I+ HI  R++AL  
Sbjct: 38  AQHARSVLHLLKETLDGVHLNSPELPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAV 97

Query: 61  RLPEFSAIFSDLPSNDFNTLFQLLHP------------AAGNRRSYFAAGVPGSFYHRLF 120
             PEFSA FSDLP NDFNTLFQLL P            AA N RSYFAAGVPGSFY RLF
Sbjct: 98  DPPEFSAFFSDLPGNDFNTLFQLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLF 157

Query: 121 PARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAG 180
           PARSI+VFHS FSLHWLSQVPESVVD  S AYN+G+V IH  N+AAAAAYR+QFQ DLAG
Sbjct: 158 PARSIDVFHSAFSLHWLSQVPESVVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAG 217

Query: 181 FLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRD 240
           FL +R+ ELK+GG+MF+ CLGRTS+DPTDQGGAG+LFGTHF+DAW+DLVQEG I+SEKRD
Sbjct: 218 FLWARAHELKRGGSMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRD 277

Query: 241 SFNIPLYAPSLQDFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSV 300
           SFNIP+YAPSLQDFKEV+EA+ SFSIN LEVFKGGSPLVVNQPDDA EVGRA+AN+CRSV
Sbjct: 278 SFNIPVYAPSLQDFKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSV 337

Query: 301 SSALVDAHIGDRLSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA 336
           S  LVDAHIGDRLS++LF+RVE RAT HAKDL EKLQFFHIVASLSLA
Sbjct: 338 SGVLVDAHIGDRLSEELFSRVERRATNHAKDLLEKLQFFHIVASLSLA 385

[Close]

BLAST of MC11g1002.1 vs. ExPASy TrEMBL
Match: A0A5D3DHL8 (Indole-3-acetate O-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G00180 PE=4 SV=1)

HSP 1 Score: 522 bits (1344), Expect = 8.30e-184
Identity = 263/348 (75.57%), Postives = 296/348 (85.06%), Query Frame = 0


Query: 1   ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN 60
           A+HAR+MLHLLKETLD VHLNSPE+PF V DLGCS G NTI  VD I+ HI  R++AL  
Sbjct: 38  AQHARSMLHLLKETLDGVHLNSPEVPFVVVDLGCSCGSNTIYIVDVIIKHIIKRFEALAV 97

Query: 61  RLPEFSAIFSDLPSNDFNTLFQLLHP------------AAGNRRSYFAAGVPGSFYHRLF 120
             PEF+A FSDLP NDFNTLFQLL P            AA N RSYFAAGVPGSFY RLF
Sbjct: 98  DPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLF 157

Query: 121 PARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAG 180
           PARSI+VFHS FSLHWLSQVPE+VVDG S+AYN+G+VFIH  N+AAA AYR+QFQ DLA 
Sbjct: 158 PARSIDVFHSAFSLHWLSQVPETVVDGRSMAYNRGRVFIHGANEAAAEAYRKQFQTDLAE 217

Query: 181 FLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRD 240
           FL +R++ELK+GG+MF+ CLGRTS+DPTDQGGAG+LFGTHF+DAW+DLVQEG I++EKRD
Sbjct: 218 FLWARAQELKRGGSMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRD 277

Query: 241 SFNIPLYAPSLQDFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSV 300
           SFNIP+YAPSLQDFKEV+EA+ SFSIN LEVFKGGSPLVVNQPDDA EVGRA+AN+CRSV
Sbjct: 278 SFNIPVYAPSLQDFKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSV 337

Query: 301 SSALVDAHIGDRLSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA 336
           S  LVDAHIGDRLS++LF RVE RAT HAKDL EKLQFFHIVASLSLA
Sbjct: 338 SGVLVDAHIGDRLSEELFYRVERRATNHAKDLLEKLQFFHIVASLSLA 385

[Close]

BLAST of MC11g1002.1 vs. ExPASy TrEMBL
Match: A0A0A0K2W3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G081680 PE=4 SV=1)

HSP 1 Score: 522 bits (1344), Expect = 8.30e-184
Identity = 262/348 (75.29%), Postives = 296/348 (85.06%), Query Frame = 0


Query: 1   ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN 60
           A+HAR+MLHLLKETLD VHLNSPE PF V DLGCS G NTI  +D I+ HI  R++AL  
Sbjct: 38  AQHARSMLHLLKETLDGVHLNSPEEPFVVVDLGCSCGSNTIYIIDVIIKHIIKRFEALAV 97

Query: 61  RLPEFSAIFSDLPSNDFNTLFQLLHP------------AAGNRRSYFAAGVPGSFYHRLF 120
             PEF+A FSDLP NDFNTLFQLL P            AA N RSYFAAGVPGSFY RLF
Sbjct: 98  DPPEFTAFFSDLPGNDFNTLFQLLPPLATYGGSMEECLAADNHRSYFAAGVPGSFYRRLF 157

Query: 121 PARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAG 180
           PARSI++FHS FSLHWLSQVPE+VVDG S+AYN+G+VFIH  N+AAA AYR+QFQ DLAG
Sbjct: 158 PARSIDLFHSAFSLHWLSQVPETVVDGRSMAYNRGRVFIHGANEAAAEAYRKQFQTDLAG 217

Query: 181 FLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRD 240
           FL +R++ELK+GG+MF+ CLGRTS+DPTDQGGAG+LFGTHF+DAW+DLVQEG I++EKRD
Sbjct: 218 FLWARAQELKRGGSMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISNEKRD 277

Query: 241 SFNIPLYAPSLQDFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSV 300
           SFNIP+YAPSLQDFKEV+EA+ SFSIN LEVFKGGSPLVVNQPDDA EVGRA+AN+CRSV
Sbjct: 278 SFNIPVYAPSLQDFKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALANSCRSV 337

Query: 301 SSALVDAHIGDRLSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA 336
           S  LVDAHIGDRLS++LF RVE RAT HAKDL EKLQFFHIVASLSLA
Sbjct: 338 SGVLVDAHIGDRLSEELFYRVERRATNHAKDLLEKLQFFHIVASLSLA 385

[Close]

BLAST of MC11g1002.1 vs. ExPASy TrEMBL
Match: A0A6J1IFI9 (indole-3-acetate O-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476788 PE=4 SV=1)

HSP 1 Score: 521 bits (1341), Expect = 2.37e-183
Identity = 265/348 (76.15%), Postives = 294/348 (84.48%), Query Frame = 0


Query: 1   ARHARAMLHLLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN 60
           A+HAR++LHLLKETLD VHLNSPELPF V DLGCS G NTI  VD I+ HI  R++AL  
Sbjct: 38  AQHARSVLHLLKETLDGVHLNSPELPFVVVDLGCSCGSNTIYIVDVIIKHIVKRFEALAL 97

Query: 61  RLPEFSAIFSDLPSNDFNTLFQLLHP------------AAGNRRSYFAAGVPGSFYHRLF 120
             PEFSA FSDLP NDFNTLFQLL P            AA N RSYFAAGVPGSFY RLF
Sbjct: 98  DPPEFSAFFSDLPGNDFNTLFQLLPPLATYGGSMEECLAANNHRSYFAAGVPGSFYRRLF 157

Query: 121 PARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAG 180
           PARSI+VFHS FSLHWLSQVPESVVD  S AYN+G+V IH  N+AAAAAYR+QFQ DLAG
Sbjct: 158 PARSIDVFHSAFSLHWLSQVPESVVDVRSTAYNRGRVSIHGANEAAAAAYRKQFQTDLAG 217

Query: 181 FLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRD 240
           FL +R+ ELK+GG+MF+ CLGRTS+DPTDQGGAG+LFGTHF+DAW+DLVQEG I+SEKRD
Sbjct: 218 FLWARAHELKRGGSMFLVCLGRTSLDPTDQGGAGLLFGTHFQDAWDDLVQEGLISSEKRD 277

Query: 241 SFNIPLYAPSLQDFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSV 300
           SFNIP+YAPSLQDFKEV+EA+ SFSIN LEVFKGGSPLVVNQPDDA EVGRA+A +CRSV
Sbjct: 278 SFNIPVYAPSLQDFKEVVEADGSFSINKLEVFKGGSPLVVNQPDDAAEVGRALAISCRSV 337

Query: 301 SSALVDAHIGDRLSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLSLA 336
           S  LVDAHIGDRLS++LF+RVE RAT HAKDL EKLQFFHIVASLSLA
Sbjct: 338 SGVLVDAHIGDRLSEELFSRVERRATNHAKDLLEKLQFFHIVASLSLA 385

[Close]

BLAST of MC11g1002.1 vs. TAIR 10
Match: AT5G55250.1 (IAA carboxylmethyltransferase 1 )

HSP 1 Score: 480.3 bits (1235), Expect = 1.2e-135
Identity = 237/347 (68.30%), Postives = 278/347 (80.12%), Query Frame = 0


Query: 1   ARHARAMLHLLKETLDIVHLNSPEL--PFTVADLGCSSGRNTITTVDFIVDHITNRYKAL 60
           A HAR+MLHLL+ETL+ VHLNS     PFT  DLGCSSG NT+  +DFIV HI+ R+ A 
Sbjct: 38  AMHARSMLHLLEETLENVHLNSSASPPPFTAVDLGCSSGANTVHIIDFIVKHISKRFDAA 97

Query: 61  GNRLPEFSAIFSDLPSNDFNTLFQLLHP-----------AAGNRRSYFAAGVPGSFYHRL 120
           G   PEF+A FSDLPSNDFNTLFQLL P           AA   RSYF AGVPGSFY RL
Sbjct: 98  GIDPPEFTAFFSDLPSNDFNTLFQLLPPLVSNTCMEECLAADGNRSYFVAGVPGSFYRRL 157

Query: 121 FPARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLA 180
           FPAR+I+ FHS FSLHWLSQVPESV D  S AYN+G+VFIH   +    AY+RQFQADLA
Sbjct: 158 FPARTIDFFHSAFSLHWLSQVPESVTDRRSAAYNRGRVFIHGAGEKTTTAYKRQFQADLA 217

Query: 181 GFLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKR 240
            FL++R+ E+K+GGAMF+ CLGRTSVDPTDQGGAG+LFGTHF+DAW+DLV+EG + +EKR
Sbjct: 218 EFLRARAAEVKRGGAMFLVCLGRTSVDPTDQGGAGLLFGTHFQDAWDDLVREGLVAAEKR 277

Query: 241 DSFNIPLYAPSLQDFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRS 300
           D FNIP+YAPSLQDFKEV++AN SF+I+ L V+KGGSPLVVN+PDDA+EVGRA A++CRS
Sbjct: 278 DGFNIPVYAPSLQDFKEVVDANGSFAIDKLVVYKGGSPLVVNEPDDASEVGRAFASSCRS 337

Query: 301 VSSALVDAHIGDRLSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLS 335
           V+  LV+AHIG+ LS KLF+RVE RAT HAKD+   LQFFHIVASLS
Sbjct: 338 VAGVLVEAHIGEELSNKLFSRVESRATSHAKDVLVNLQFFHIVASLS 384

[Close]

BLAST of MC11g1002.1 vs. TAIR 10
Match: AT5G55250.2 (IAA carboxylmethyltransferase 1 )

HSP 1 Score: 479.2 bits (1232), Expect = 2.8e-135
Identity = 236/345 (68.41%), Postives = 277/345 (80.29%), Query Frame = 0


Query: 3   HARAMLHLLKETLDIVHLNSPEL--PFTVADLGCSSGRNTITTVDFIVDHITNRYKALGN 62
           HAR+MLHLL+ETL+ VHLNS     PFT  DLGCSSG NT+  +DFIV HI+ R+ A G 
Sbjct: 2   HARSMLHLLEETLENVHLNSSASPPPFTAVDLGCSSGANTVHIIDFIVKHISKRFDAAGI 61

Query: 63  RLPEFSAIFSDLPSNDFNTLFQLLHP-----------AAGNRRSYFAAGVPGSFYHRLFP 122
             PEF+A FSDLPSNDFNTLFQLL P           AA   RSYF AGVPGSFY RLFP
Sbjct: 62  DPPEFTAFFSDLPSNDFNTLFQLLPPLVSNTCMEECLAADGNRSYFVAGVPGSFYRRLFP 121

Query: 123 ARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAGF 182
           AR+I+ FHS FSLHWLSQVPESV D  S AYN+G+VFIH   +    AY+RQFQADLA F
Sbjct: 122 ARTIDFFHSAFSLHWLSQVPESVTDRRSAAYNRGRVFIHGAGEKTTTAYKRQFQADLAEF 181

Query: 183 LQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRDS 242
           L++R+ E+K+GGAMF+ CLGRTSVDPTDQGGAG+LFGTHF+DAW+DLV+EG + +EKRD 
Sbjct: 182 LRARAAEVKRGGAMFLVCLGRTSVDPTDQGGAGLLFGTHFQDAWDDLVREGLVAAEKRDG 241

Query: 243 FNIPLYAPSLQDFKEVIEANSSFSINMLEVFKGGSPLVVNQPDDATEVGRAMANTCRSVS 302
           FNIP+YAPSLQDFKEV++AN SF+I+ L V+KGGSPLVVN+PDDA+EVGRA A++CRSV+
Sbjct: 242 FNIPVYAPSLQDFKEVVDANGSFAIDKLVVYKGGSPLVVNEPDDASEVGRAFASSCRSVA 301

Query: 303 SALVDAHIGDRLSKKLFTRVEHRATCHAKDLFEKLQFFHIVASLS 335
             LV+AHIG+ LS KLF+RVE RAT HAKD+   LQFFHIVASLS
Sbjct: 302 GVLVEAHIGEELSNKLFSRVESRATSHAKDVLVNLQFFHIVASLS 346

[Close]

BLAST of MC11g1002.1 vs. TAIR 10
Match: AT5G56300.1 (gibberellic acid methyltransferase 2 )

HSP 1 Score: 221.1 bits (562), Expect = 1.4e-57
Identity = 131/327 (40.06%), Postives = 175/327 (53.52%), Query Frame = 0


Query: 10  LLKETLDIVHLNSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKAL--GNRLPEFSA 69
           +L  +L  + L S + P  + DLGC++G NT TTVD +V+ +  RY A   G   PEF A
Sbjct: 50  ILISSLQSIKLFSDQTPIKITDLGCATGSNTFTTVDTVVETLQRRYTARCGGGGSPEFEA 109

Query: 70  IFSDLPSNDFNTLFQLL--HPAAGNRRSYFAAGVPGSFYHRLFPARSINVFHSVFSLHWL 129
            F DLPSNDFN LF+LL       +   YFA GV GSFY RLFP  +I+V  S+ +LHWL
Sbjct: 110 FFCDLPSNDFNMLFKLLAEKQKVDSPAKYFAGGVAGSFYDRLFPRGTIHVAVSLSALHWL 169

Query: 130 SQVPESVVDGGSVAYNKGKVFIHRGNKAAAAAYRRQFQADLAGFLQSRSEELKKGGAMFV 189
           SQ+PE V++  S  +NKGK +I    K    AY  Q   DL  F+  R EE+ KGG +FV
Sbjct: 170 SQIPEKVLEKESRTWNKGKTWIEGAKKEVVEAYAEQSDKDLDDFMSCRKEEMVKGGVLFV 229

Query: 190 ACLGRTSVDPTDQGG----AGILFGTHFEDAWEDLVQEGWITSEKRDSFNIPLYAPSLQD 249
              GR S   +  G     A   F T  E AW+DL++EG I  E RD FNIP Y  S ++
Sbjct: 230 LMAGRPSGSSSQFGDQDTRAKHPFTTTMEQAWQDLIEEGLIDEETRDGFNIPAYMRSPEE 289

Query: 250 FKEVIEANSSFSINMLEVFKGGSPLVVNQPD--------DATEVGRAMANTCRSVSSALV 309
               I+    F I  ++  K     +V   D        D    GRA  N  ++    +V
Sbjct: 290 VTAGIDRCGGFKIGKMDFLK-----IVEYSDEKQEEWKKDPVSYGRARTNLVQAAIRPMV 349

Query: 310 DAHIGDRLSKKLFTRVEHRATCHAKDL 321
           DA++G  LS +LF R E+R + + + L
Sbjct: 350 DAYLGPDLSHELFKRYENRVSTNQEFL 371

[Close]

BLAST of MC11g1002.1 vs. TAIR 10
Match: AT4G26420.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 195.7 bits (496), Expect = 6.1e-50
Identity = 123/317 (38.80%), Postives = 168/317 (53.00%), Query Frame = 0


Query: 29  VADLGCSSGRNTITTVDFIVDHITNRYKAL-GNRLP--EFSAIFSDLPSNDFNTLFQLL- 88
           +ADLGC+ G NT +TV+ +V+ +  +   + G   P  EF   FSDL SNDFN LF+ L 
Sbjct: 59  IADLGCAIGDNTFSTVETVVEVLGKKLAVIDGGTEPEMEFEVFFSDLSSNDFNALFRSLD 118

Query: 89  HPAAGNRRSYFAAGVPGSFYHRLFPARSINVFHSVFSLHWLSQVPESVVDGGSVAYNKGK 148
               G+ R YFAAGVPGSFY RLFP   ++V  ++ +L WLSQVPE V++ GS ++NKG 
Sbjct: 119 EKVNGSSRKYFAAGVPGSFYKRLFPKGELHVVVTMSALQWLSQVPEKVMEKGSKSWNKGG 178

Query: 149 VFIHRGNKAAAAAYRRQFQADLAGFLQSRSEELKKGGAMFVACLGRTSVDPTDQGGAGIL 208
           V+I    K    AY  Q   DL  FL+ R EE+  GG +F+   GR S      G     
Sbjct: 179 VWIEGAEKEVVEAYAEQADKDLVEFLKCRKEEIVVGGVLFMLMGGRPSGSVNQIGDPDSS 238

Query: 209 ----FGTHFEDAWEDLVQEGWITSEKRDSFNIPLYAPSLQDFKEVIEANSSFSINMLE-- 268
               F T  + AW+DLV EG I  EKRD FNIP+Y  + ++    I+    F I   E  
Sbjct: 239 LKHPFTTLMDQAWQDLVDEGLIEEEKRDGFNIPVYFRTTEEIAAAIDRCGGFKIEKTENL 298

Query: 269 ----VFKGGSPLVVNQPDDATEVGRAMANTCRSVSSALVDAHIGDRLSKKLFTRVEHRAT 328
                  G    ++  PD     GR  AN  ++    +V A++G  L+ KLF R   RA 
Sbjct: 299 IIADHMNGKQEELMKDPD---SYGRDRANYAQAGLKPIVQAYLGPDLTHKLFKRYAVRAA 358

Query: 329 CHAKDLFEKLQFFHIVA 332
              K++     F+H++A
Sbjct: 359 AD-KEILNNC-FYHMIA 370

[Close]

BLAST of MC11g1002.1 vs. TAIR 10
Match: AT1G19640.1 (jasmonic acid carboxyl methyltransferase )

HSP 1 Score: 188.0 bits (476), Expect = 1.3e-47
Identity = 124/318 (38.99%), Postives = 169/318 (53.14%), Query Frame = 0


Query: 21  NSPELPFTVADLGCSSGRNTITTVDFIVDHITNRYKALGNRLPEFSAIFSDLPSNDFNTL 80
           NS      +ADLGCSSG N++ ++  IVD I N    L   +PE     +DLPSNDFN +
Sbjct: 47  NSEISSIGIADLGCSSGPNSLLSISNIVDTIHNLCPDLDRPVPELRVSLNDLPSNDFNYI 106

Query: 81  FQLLHP--------------AAGNRRSYFAAGVPGSFYHRLFPARSINVFHSVFSLHWLS 140
              L                  G   S F + VPGSFY RLFP RS++  HS  SLHWLS
Sbjct: 107 CASLPEFYDRVNNNKEGLGFGRGGGESCFVSAVPGSFYGRLFPRRSLHFVHSSSSLHWLS 166

Query: 141 QVP--ESVVDGGSVA---YNKGKVFIHRGN-KAAAAAYRRQFQADLAGFLQSRSEELKKG 200
           QVP  E+  +  ++     N GK++I + + K+A  AY  QFQ D   FL+SRSEEL  G
Sbjct: 167 QVPCREAEKEDRTITADLENMGKIYISKTSPKSAHKAYALQFQTDFWVFLRSRSEELVPG 226

Query: 201 GAMFVACLGRTSVDPTDQGGAGILFGTHFEDAWEDLVQEGWITSEKRDSFNIPLYAPSLQ 260
           G M ++ LGR S+DPT +             A   + +EG I  EK D+FN P YA S +
Sbjct: 227 GRMVLSFLGRRSLDPTTE--ESCYQWELLAQALMSMAKEGIIEEEKIDAFNAPYYAASSE 286

Query: 261 DFKEVIEANSSFSINMLEV----FKGGS------PLVVNQPDDATEVGRAMANTCRSVSS 309
           + K VIE   SFSI+ LE+    ++GGS       LV+    +A   GR ++NT R+V  
Sbjct: 287 ELKMVIEKEGSFSIDRLEISPIDWEGGSISEESYDLVIRSKPEALASGRRVSNTIRAVVE 346

The following BLAST results are available for this feature:

BLAST of MC11g1002.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLN8	1.7e-134	68.30	Indole-3-acetate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=IAMT1 ...	[more]
Q0J998	1.9e-120	60.85	Indole-3-acetate O-methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q5XF78	1.9e-56	40.06	Gibberellic acid methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GAMT2 PE...	[more]
A0A061DFA8	3.0e-49	38.01	Probable methyltransferase TCM_000168 OS=Theobroma cacao OX=3641 GN=TCM_000168 P...	[more]
F4JUY5	8.6e-49	38.80	Gibberellic acid methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=GAMT1 PE...	[more]

BLAST of MC11g1002.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139646.1	2.66e-242	100.00	indole-3-acetate O-methyltransferase 1-like [Momordica charantia]	[more]
KAG7024938.1	2.97e-184	76.44	Indole-3-acetate O-methyltransferase 1, partial [Cucurbita argyrosperma subsp. a...	[more]
KAG6592061.1	4.22e-184	76.15	Indole-3-acetate O-methyltransferase 1, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023536087.1	5.99e-184	76.15	indole-3-acetate O-methyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022936266.1	5.99e-184	76.15	indole-3-acetate O-methyltransferase 1-like [Cucurbita moschata]	[more]

BLAST of MC11g1002.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDB0	1.29e-242	100.00	indole-3-acetate O-methyltransferase 1-like OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1F711	2.90e-184	76.15	indole-3-acetate O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A5D3DHL8	8.30e-184	75.57	Indole-3-acetate O-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0K2W3	8.30e-184	75.29	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G081680 PE=4 SV=1	[more]
A0A6J1IFI9	2.37e-183	76.15	indole-3-acetate O-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]

BLAST of MC11g1002.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55250.1	1.2e-135	68.30	IAA carboxylmethyltransferase 1	[more]
AT5G55250.2	2.8e-135	68.41	IAA carboxylmethyltransferase 1	[more]
AT5G56300.1	1.4e-57	40.06	gibberellic acid methyltransferase 2	[more]
AT4G26420.1	6.1e-50	38.80	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G19640.1	1.3e-47	38.99	jasmonic acid carboxyl methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR042086	Methyltransferase, alpha-helical capping domain	GENE3D	1.10.1200.270		coord: 127..317 e-value: 6.8E-110 score: 369.1
IPR005299	SAM dependent carboxyl methyltransferase	PFAM	PF03492	Methyltransf_7	coord: 25..334 e-value: 5.6E-103 score: 344.7
IPR005299	SAM dependent carboxyl methyltransferase	PANTHER	PTHR31009	S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN	coord: 1..335
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 3..263 e-value: 6.8E-110 score: 369.1
None	No IPR available	PANTHER	PTHR31009:SF42	INDOLE-3-ACETATE O-METHYLTRANSFERASE 1	coord: 1..335
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 7..334

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC11g1002	MC11g1002	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g1002.1-cds	MC11g1002.1-cds-MC11:8588184..8588534	CDS
MC11g1002.1-cds	MC11g1002.1-cds-MC11:8588743..8589399	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC11g1002.1	MC11g1002.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity