MC11g0131.1 (mRNA) Bitter gourd (Dali-11) v1

BLAST of MC11g0131.1 vs. NCBI nr
Match: KAG6608571.1 (hypothetical protein SDJN03_01913, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 194 bits (494), Expect = 1.83e-59
Identity = 116/182 (63.74%), Postives = 126/182 (69.23%), Query Frame = 0

Query: 14  VVFAALIYGSMADGLKNGTMRTNVTFLTFSE------GRNESSSNTTTTVEVSKQNGSGR 73
           V F   +YGS A+GLKNGT      FLTFSE      G   +SS  TTTVEVSKQN   R
Sbjct: 53  VFFGVAVYGSRAEGLKNGT------FLTFSELPRGNAGEGRNSSGNTTTVEVSKQN---R 112

Query: 74  GGGGGYGWGWGWGGGGGGGGGG-----------------WWKWGCGGQPKSGRGGGGRRR 133
           GGGGGYGWGWG GGGGGGGGGG                 WWKWGCGGQPKSGR GGGRR+
Sbjct: 113 GGGGGYGWGWGGGGGGGGGGGGGGGGGGGGWGWGGGGGGWWKWGCGGQPKSGRRGGGRRK 172

Query: 134 GSGRNQNHVFRKRMYDEGDYKTGEFGQCVVRGRCRGMRLDCPLHCGGPCFYDCRYMCKAH 172
                  +V RKR+Y+E DY  GEF +C+V+GRCRGMRLDCPLHCGGPCFYDCRYMCKAH
Sbjct: 173 -------NVDRKRIYNEEDYSMGEFAECMVKGRCRGMRLDCPLHCGGPCFYDCRYMCKAH 218

BLAST of MC11g0131.1 vs. NCBI nr
Match: KAG7037889.1 (hypothetical protein SDJN02_01520, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 192 bits (488), Expect = 1.02e-58
Identity = 115/187 (61.50%), Postives = 126/187 (67.38%), Query Frame = 0

Query: 14  VVFAALIYGSMADGLKNGTMRTNVTFLTFSE------GRNESSSNTTTTVEVSKQNGSGR 73
           + F   +YGS A+GLKNGT      FLTFSE      G   +SS  TTTVEVSKQN   R
Sbjct: 36  IFFGVAVYGSRAEGLKNGT------FLTFSELPRGNAGEGRNSSGNTTTVEVSKQN---R 95

Query: 74  GGGGGYGWGWGWGGGGGGGGGG----------------------WWKWGCGGQPKSGRGG 133
           GGGGGYGWGWG GGGGGGGGGG                      WWKWGCGGQPKSGR G
Sbjct: 96  GGGGGYGWGWGGGGGGGGGGGGGGGGGGGGGGGGGWGWGGGGGGWWKWGCGGQPKSGRRG 155

Query: 134 GGRRRGSGRNQNHVFRKRMYDEGDYKTGEFGQCVVRGRCRGMRLDCPLHCGGPCFYDCRY 172
           GGRR+       +V RKR+Y+E DY  GEF +C+V+GRCRGMRLDCPLHCGGPCFYDCRY
Sbjct: 156 GGRRK-------NVDRKRIYNEEDYSMGEFAECMVKGRCRGMRLDCPLHCGGPCFYDCRY 206

BLAST of MC11g0131.1 vs. NCBI nr
Match: XP_022940700.1 (glycine-rich RNA-binding protein 2-like [Cucurbita moschata])

HSP 1 Score: 190 bits (483), Expect = 6.23e-58
Identity = 113/175 (64.57%), Postives = 124/175 (70.86%), Query Frame = 0

Query: 20  IYGSMADGLKNGTMRTNVTFLTFSE------GRNESSSNTTTTVEVSKQNGSGRGGGGGY 79
           +YGS A+GLKNGT      FLTFSE      G   +SS  TTTVEVSKQN   RGGGGGY
Sbjct: 54  VYGSRAEGLKNGT------FLTFSELPGGNAGEGRNSSGNTTTVEVSKQN---RGGGGGY 113

Query: 80  GWGWGWGGGGGGGGGG----------------WWKWGCGGQPKSGRGGGGRRRGSGRNQN 139
           GWGWG GGGGGGGGGG                WWKWGCGGQPKSGR GGGRR+       
Sbjct: 114 GWGWGGGGGGGGGGGGGGGGGGGWGWGGGGGGWWKWGCGGQPKSGRRGGGRRK------- 173

Query: 140 HVFRKRMYDEGDYKTGEFGQCVVRGRCRGMRLDCPLHCGGPCFYDCRYMCKAHCR 172
           +V RKR+Y+E DY  GEF +C+V+GRCRGMRLDCPLHCGGPCFYDCR+MCKAHCR
Sbjct: 174 NVDRKRIYNEEDYSLGEFAECMVKGRCRGMRLDCPLHCGGPCFYDCRHMCKAHCR 212

BLAST of MC11g0131.1 vs. NCBI nr
Match: XP_008455258.1 (PREDICTED: keratin, type II cytoskeletal 1 [Cucumis melo])

HSP 1 Score: 189 bits (480), Expect = 1.01e-57
Identity = 121/197 (61.42%), Postives = 139/197 (70.56%), Query Frame = 0

Query: 1   MACQNLMKFCALAVVFAALIYGSMA-DGLKNGTMRTNVTFLTFSE------GRNESSSNT 60
           M  +N +K  AL V+ A +IYGS A +GL NG+M  NVTFLTFSE      G   +SS+ 
Sbjct: 1   MGWENPIKLSAL-VILATVIYGSRAAEGLGNGSMPINVTFLTFSELPQRNAGERRNSSSN 60

Query: 61  TTTVEVSKQNGSGRGGGGGYGWGWGWGGGGGGGGGG-------------------WWKWG 120
           T+TVEVSKQNG G GGGGGYGWGWG GGGGGGGGGG                   WWKWG
Sbjct: 61  TSTVEVSKQNGGG-GGGGGYGWGWGSGGGGGGGGGGGGGGGGGGGGWGWGGGGGGWWKWG 120

Query: 121 CGGQPKSGRGGGGRRRGSGRNQNHVFRKRMYDEGDYKTGEFGQCVVRGRCRGMRLDCPLH 171
           CGG+PK  +   GRR  +GR +N V RK+MY+E DY+ GEF QC+V+GRCRGMRLDCPLH
Sbjct: 121 CGGRPKFEK--VGRRDDNGRRKN-VKRKKMYNEKDYRMGEFAQCMVKGRCRGMRLDCPLH 180

BLAST of MC11g0131.1 vs. NCBI nr
Match: XP_011659711.1 (keratin, type II cytoskeletal 2 epidermal [Cucumis sativus] >KGN43666.1 hypothetical protein Csa_017107 [Cucumis sativus])

HSP 1 Score: 186 bits (472), Expect = 1.46e-56
Identity = 121/194 (62.37%), Postives = 136/194 (70.10%), Query Frame = 0

Query: 1   MACQNLMKFCALAVVFAALIYGSMA-DGLKNGTMRTNVTFLTFSE------GRNESSSNT 60
           M  +N +K  AL V+ A +I GS A +GL NG+M  NVTFLTFSE      G   +SS+ 
Sbjct: 1   MGWENPIKLSAL-VILATVISGSRAAEGLGNGSMPMNVTFLTFSELPQRNAGERRNSSSN 60

Query: 61  TTTVEVSKQNGSGRGGGGGYGWGWGWGGGGGGGGGG----------------WWKWGCGG 120
           +TTVEVSKQNG G  GGGGYGWGWG GGGGGGGGGG                WWKWGCGG
Sbjct: 61  STTVEVSKQNGGG--GGGGYGWGWGSGGGGGGGGGGGGGGGGGWGWGGGGGGWWKWGCGG 120

Query: 121 QPKSGRGGGGRRRGSGRNQNHVFRKRMYDEGDYKTGEFGQCVVRGRCRGMRLDCPLHCGG 171
           QPKS R   GRR  +G ++N V  KRMY E DY+ GEF QC+V+GRCRGMRLDCPLHCGG
Sbjct: 121 QPKSER--VGRRDDNGGSRN-VKGKRMYSEKDYRMGEFAQCMVKGRCRGMRLDCPLHCGG 180

BLAST of MC11g0131.1 vs. ExPASy TrEMBL
Match: A0A6J1FRC8 (glycine-rich RNA-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111446212 PE=4 SV=1)

HSP 1 Score: 190 bits (483), Expect = 3.01e-58
Identity = 113/175 (64.57%), Postives = 124/175 (70.86%), Query Frame = 0

Query: 20  IYGSMADGLKNGTMRTNVTFLTFSE------GRNESSSNTTTTVEVSKQNGSGRGGGGGY 79
           +YGS A+GLKNGT      FLTFSE      G   +SS  TTTVEVSKQN   RGGGGGY
Sbjct: 54  VYGSRAEGLKNGT------FLTFSELPGGNAGEGRNSSGNTTTVEVSKQN---RGGGGGY 113

Query: 80  GWGWGWGGGGGGGGGG----------------WWKWGCGGQPKSGRGGGGRRRGSGRNQN 139
           GWGWG GGGGGGGGGG                WWKWGCGGQPKSGR GGGRR+       
Sbjct: 114 GWGWGGGGGGGGGGGGGGGGGGGWGWGGGGGGWWKWGCGGQPKSGRRGGGRRK------- 173

Query: 140 HVFRKRMYDEGDYKTGEFGQCVVRGRCRGMRLDCPLHCGGPCFYDCRYMCKAHCR 172
           +V RKR+Y+E DY  GEF +C+V+GRCRGMRLDCPLHCGGPCFYDCR+MCKAHCR
Sbjct: 174 NVDRKRIYNEEDYSLGEFAECMVKGRCRGMRLDCPLHCGGPCFYDCRHMCKAHCR 212

BLAST of MC11g0131.1 vs. ExPASy TrEMBL
Match: A0A1S3C029 (keratin, type II cytoskeletal 1 OS=Cucumis melo OX=3656 GN=LOC103495464 PE=4 SV=1)

HSP 1 Score: 189 bits (480), Expect = 4.89e-58
Identity = 121/197 (61.42%), Postives = 139/197 (70.56%), Query Frame = 0

Query: 1   MACQNLMKFCALAVVFAALIYGSMA-DGLKNGTMRTNVTFLTFSE------GRNESSSNT 60
           M  +N +K  AL V+ A +IYGS A +GL NG+M  NVTFLTFSE      G   +SS+ 
Sbjct: 1   MGWENPIKLSAL-VILATVIYGSRAAEGLGNGSMPINVTFLTFSELPQRNAGERRNSSSN 60

Query: 61  TTTVEVSKQNGSGRGGGGGYGWGWGWGGGGGGGGGG-------------------WWKWG 120
           T+TVEVSKQNG G GGGGGYGWGWG GGGGGGGGGG                   WWKWG
Sbjct: 61  TSTVEVSKQNGGG-GGGGGYGWGWGSGGGGGGGGGGGGGGGGGGGGWGWGGGGGGWWKWG 120

Query: 121 CGGQPKSGRGGGGRRRGSGRNQNHVFRKRMYDEGDYKTGEFGQCVVRGRCRGMRLDCPLH 171
           CGG+PK  +   GRR  +GR +N V RK+MY+E DY+ GEF QC+V+GRCRGMRLDCPLH
Sbjct: 121 CGGRPKFEK--VGRRDDNGRRKN-VKRKKMYNEKDYRMGEFAQCMVKGRCRGMRLDCPLH 180

BLAST of MC11g0131.1 vs. ExPASy TrEMBL
Match: A0A0A0K766 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G056450 PE=4 SV=1)

HSP 1 Score: 186 bits (472), Expect = 7.05e-57
Identity = 121/194 (62.37%), Postives = 136/194 (70.10%), Query Frame = 0

Query: 1   MACQNLMKFCALAVVFAALIYGSMA-DGLKNGTMRTNVTFLTFSE------GRNESSSNT 60
           M  +N +K  AL V+ A +I GS A +GL NG+M  NVTFLTFSE      G   +SS+ 
Sbjct: 1   MGWENPIKLSAL-VILATVISGSRAAEGLGNGSMPMNVTFLTFSELPQRNAGERRNSSSN 60

Query: 61  TTTVEVSKQNGSGRGGGGGYGWGWGWGGGGGGGGGG----------------WWKWGCGG 120
           +TTVEVSKQNG G  GGGGYGWGWG GGGGGGGGGG                WWKWGCGG
Sbjct: 61  STTVEVSKQNGGG--GGGGYGWGWGSGGGGGGGGGGGGGGGGGWGWGGGGGGWWKWGCGG 120

Query: 121 QPKSGRGGGGRRRGSGRNQNHVFRKRMYDEGDYKTGEFGQCVVRGRCRGMRLDCPLHCGG 171
           QPKS R   GRR  +G ++N V  KRMY E DY+ GEF QC+V+GRCRGMRLDCPLHCGG
Sbjct: 121 QPKSER--VGRRDDNGGSRN-VKGKRMYSEKDYRMGEFAQCMVKGRCRGMRLDCPLHCGG 180

BLAST of MC11g0131.1 vs. ExPASy TrEMBL
Match: A0A5D2KXD0 (Uncharacterized protein OS=Gossypium tomentosum OX=34277 GN=ES332_D05G207300v1 PE=4 SV=1)

HSP 1 Score: 141 bits (355), Expect = 5.78e-39
Identity = 84/114 (73.68%), Postives = 95/114 (83.33%), Query Frame = 0

Query: 64  GSGRGGGGGYGWGWGWGGGG---GGGGGGWWKWGCGGQPKSGRGGG-GRRRGS-GRNQNH 123
           G G GGGGGYGWGWG GGGG   GGGGGGW+KWGCGG+ K+G+G G GR +GS GRNQ H
Sbjct: 96  GGGGGGGGGYGWGWGGGGGGWGWGGGGGGWYKWGCGGR-KAGKGNGRGRDKGSIGRNQAH 155

Query: 124 VFRKRMYDEGDYKTGEFGQCVVRGRCRGMRLDCPLHCGGPCFYDCRYMCKAHCR 172
             RKR++++ DYK GEF QC VRGRCRGMRLDCPLHCGGPC YDC++MCKAHCR
Sbjct: 156 --RKRVFNKEDYKMGEFAQCTVRGRCRGMRLDCPLHCGGPCVYDCQHMCKAHCR 206

BLAST of MC11g0131.1 vs. ExPASy TrEMBL
Match: A0A5J5RFA8 (Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=ES319_D05G197900v1 PE=4 SV=1)

HSP 1 Score: 140 bits (354), Expect = 8.41e-39
Identity = 84/115 (73.04%), Postives = 95/115 (82.61%), Query Frame = 0

Query: 64  GSGRGGGGGYGWGWGWGGGGG----GGGGGWWKWGCGGQPKSGRGGG-GRRRGS-GRNQN 123
           G G GGGGGYGWGWG GGGGG    GGGGGW+KWGCGG+ K+G+G G GR +GS GRNQ 
Sbjct: 96  GGGGGGGGGYGWGWGGGGGGGWGWGGGGGGWYKWGCGGR-KAGKGNGRGRDKGSIGRNQA 155

Query: 124 HVFRKRMYDEGDYKTGEFGQCVVRGRCRGMRLDCPLHCGGPCFYDCRYMCKAHCR 172
           H  RKR++++ DYK GEF QC VRGRCRGMRLDCPLHCGGPC YDC++MCKAHCR
Sbjct: 156 H--RKRVFNKEDYKMGEFAQCTVRGRCRGMRLDCPLHCGGPCVYDCQHMCKAHCR 207

BLAST of MC11g0131.1 vs. TAIR 10
Match: AT1G75550.1 (glycine-rich protein )

HSP 1 Score: 81.3 bits (199), Expect = 8.6e-16
Identity = 76/180 (42.22%), Postives = 91/180 (50.56%), Query Frame = 0

Query: 7   MKFCALAVVFAALIYGSMADGLKNGTMRTNVTFLTFSEGRNESSSNTTTTVEVSKQNGSG 66
           MK  +  VV   LI     + L +G  R+N       +  N   ++TTT V V  +  S 
Sbjct: 1   MKTISSLVVIFLLILIFQTNKLVSGHDRSNDQDTV--KNNNNDENSTTTGVVVRDKKRSY 60

Query: 67  RGGGGGYGWGW----------GWGGGGGGGGGGWWKWGCGGQP----KSGRGGGGRRRGS 126
            GG G Y WGW          G GGGGGGGGGG W WG GG      K G GGGG  +G 
Sbjct: 61  NGGSGSYRWGWGGGGGGGGGGGGGGGGGGGGGGGWGWGGGGGGGGWYKWGCGGGG--KGK 120

Query: 127 GRNQNHVFRKRMYDEGDYKTGEFGQCVVRGRCRGMRLDCPLHCGGPCFYDCRYMCKAHCR 173
           GR     F KR          E+ +C  RG+C G RL+CP HCGG CFYDC ++CK HCR
Sbjct: 121 GREGRGEFVKR----------EYAECKGRGKCSGKRLECPQHCGGLCFYDCLFLCKPHCR 166