MC11g0064.1 (mRNA) Bitter gourd (Dali-11) v1

BLAST of MC11g0064.1 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 5.3e-221
Identity = 355/549 (64.66%), Postives = 445/549 (81.06%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           M+G  +  AN+ PLTPI+FL+RSA VYADR+S+VYG ++YTWR T  RC R+AS L +LG
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           I+ GDVV+ LAPNVPAM ELHF VPMAGA+LCTLNIRH++++V+ LL HS  K+I  D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPDHLEFESLLATGEVDFKIRR 180
           FL + +GA +++  + +K   LV+I +   Q         + +E+E ++A G+ DF++ R
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 PRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMFHCNG 240
           P DE D IS+NYTSGTTS PKGV+YSHRGAYLN+L+ VLLN+M   P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQKPLPG 300
           WC  WGV A GGTNIC R VTAK IF NIS+H VTHMGGAPT+LNMIINAP  EQKPLPG
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 KVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDKQAKLN 360
           KV+ +TG APPP+HV+ KM  +GF + H+YGLTETYG  T+C+W+PEWDSL  ++QAK+ 
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 SRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFSGGW 420
           +RQG+ H+G+EE+ +K+P+TM+++PADG TMGEV+FRGNTVMNGYLK+ +AT+EAF GGW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGRPDDHW 480
           F SGDLGV+HP+GYIELKDRSKDIIISGGENISSIEVES LF+HP +LEAAVV RPD++W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFILKEEA 540
           GET CAFVKLKDG  AS EE+I +CR+ L HYMAPR +VF+DLPKTSTGK QKF+L+ +A
Sbjct: 483 GETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKA 542

Query: 541 RAMGSLPKR 550
           +A+ SL K+
Sbjct: 543 KALVSLSKK 551

BLAST of MC11g0064.1 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 2.3e-208
Identity = 345/558 (61.83%), Postives = 431/558 (77.24%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           ++G+ R  AN +PL+PITFLERSA VY DR SLV+G +++TW  T QRC RLAS L  LG
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           I+RGDVVAALAPNVPAM+ELHFAVPMAG +LC LN R + + +S LL HSEAKI+ VD+Q
Sbjct: 108 ISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQ 167

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPD-----------HLEFESLL 180
            L +  GA+ ++ +  + +  L +++    Q    D  D D             E+E+LL
Sbjct: 168 LLEIAHGALDLLAKSDKTRKSLKLVL--ISQSNDDDDSDEDSSSTFASKYSFDYEYETLL 227

Query: 181 ATGEVDFKIRRPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVY 240
            +G+ +F+I +PR EWDPIS+NYTSGTTSRPKGV+YSHRGAYLN+L+TV L+ MS  PVY
Sbjct: 228 KSGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVY 287

Query: 241 LWTVPMFHCNGWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIIN 300
           LWTVPMFHCNGWC  WGVAAQGGTNIC R V+ K IF NI+ H VTHMGGAPTVLNMI+N
Sbjct: 288 LWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVN 347

Query: 301 APIIEQKPLPGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWD 360
             + E KPLP +V +MTGG+PP   +L KM  +GF V H YGLTETYG  T C W+PEWD
Sbjct: 348 YTVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWD 407

Query: 361 SLHEDKQAKLNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDL 420
           SL  +++ KL +RQG+QH+G+E +D+K+P+TM++VP DG TMGEVMFRGNTVM+GY KD+
Sbjct: 408 SLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDI 467

Query: 421 KATREAFSGGWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILE 480
           +ATR+AF G WF SGDL V++P+GYIE+KDR KD+IISGGENISS+EVE VL SH ++LE
Sbjct: 468 EATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLE 527

Query: 481 AAVVGRPDDHWGETPCAFVKLKDG-RSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTST 540
           AAVV RPD HWG+TPC FVKLK+G  +   EEII FCR+HL HYMAP+ +VF D+PKTST
Sbjct: 528 AAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTST 587

Query: 541 GKTQKFILKEEARAMGSL 547
           GK QK++L+++A  MGSL
Sbjct: 588 GKVQKYLLRKKADEMGSL 603

BLAST of MC11g0064.1 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 2.1e-201
Identity = 342/550 (62.18%), Postives = 423/550 (76.91%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVL-VRL 60
           M+G+ +CSANH PL+PITFLERS+  Y D  SLVYG +RYTW  T  RC +LAS L   L
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHL 88

Query: 61  GIARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDY 120
           GI+ GDVVA  + N+P +YELHFAVPMAG +LCTLN R+++AMVSTLL HSEAK+I V+ 
Sbjct: 89  GISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QFLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPDHLEFESLLATGEVDFKIR 180
           Q L   + A+ ++  +++ K P +V++ +  +   S S D     +  LLA G  DF+IR
Sbjct: 149 QLLETARAALDLL-AQKDIKPPTLVLLTD-SESFTSSSYD----HYNHLLANGSDDFEIR 208

Query: 181 RPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMFHCN 240
           RP++EWDPIS+NYTSGTT+RPK V+YSHRGAYLN+++TVLL+ M    VYLW+VPMFHCN
Sbjct: 209 RPKNEWDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTTSVYLWSVPMFHCN 268

Query: 241 GWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIE-QKPL 300
           GWCF WG AAQG TNIC R V+ K IF NI  H VTH G APTVLNMI+N+P      PL
Sbjct: 269 GWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPL 328

Query: 301 PGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDKQAK 360
           P KV VMTGG+PPP  V+ +M  MGF V H YGLTET G A  C  +PEWD+L  +++  
Sbjct: 329 PHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERYA 388

Query: 361 LNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFSG 420
           L +RQGL H+ MEEMD+++P+TM+SV ADG T+GEVMFRGNTVM+GY KDLKAT EAF G
Sbjct: 389 LKARQGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEAFEG 448

Query: 421 GWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGRPDD 480
           GWFRSGDLGV+H +GYI+LKDR KD++ISGGENIS++EVE+VL+SH ++LEAAVV RPD 
Sbjct: 449 GWFRSGDLGVKHEDGYIQLKDRKKDVVISGGENISTVEVETVLYSHEAVLEAAVVARPDK 508

Query: 481 HWGETPCAFVKLKDG--RSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFIL 540
            WGETPCAFV LK+G     S ++IIKFCR+ L HYMAP+ VVF++LPKTSTGK QK+IL
Sbjct: 509 LWGETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYIL 568

Query: 541 KEEARAMGSL 547
           KE+A AMGSL
Sbjct: 569 KEKAMAMGSL 572

BLAST of MC11g0064.1 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 5.1e-200
Identity = 338/550 (61.45%), Postives = 424/550 (77.09%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVL-VRL 60
           M+G+ +CSANH PL+PITFLERS+  Y D  SLVYG +RYTW  T  RC +LAS L    
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHF 88

Query: 61  GIARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDY 120
           GI+ GDVVA  + N+P +YELHFAVPMAG +LCTLN R+++AMVSTLL HSEAK+I V+ 
Sbjct: 89  GISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QFLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPDHLEFESLLATGEVDFKIR 180
           Q L   + A+ ++  +++ K P +V++ +  +   S S D     +  LLA G  DF+IR
Sbjct: 149 QLLETARAALDLL-AQKDIKPPTLVLLTD-SESFTSSSYD----HYNHLLANGSDDFEIR 208

Query: 181 RPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMFHCN 240
           RP++E DPIS+NYTSGTT+RPK V+YSHRGAYLN+++TVLL+ M    VYLW+VPMFHCN
Sbjct: 209 RPKNECDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTRSVYLWSVPMFHCN 268

Query: 241 GWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIE-QKPL 300
           GWCF WG AAQG TNIC R V+ K IF NI  H VTH G APTVLNMI+N+P      PL
Sbjct: 269 GWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPL 328

Query: 301 PGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDKQAK 360
           P KV VMTGG+PPP  V+ +M  MGF V H YGLTET+G AT C  +PEWD+L  +++  
Sbjct: 329 PHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEERYA 388

Query: 361 LNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFSG 420
           L +RQGL H+ MEEMD+++P++M+SV ADG T+GEVMFRGNTVM+GY KDLKAT EAF G
Sbjct: 389 LKARQGLNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEAFEG 448

Query: 421 GWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGRPDD 480
           GWFR+GDLGV+H +GYI+LKDR KD++ISGGEN+S++EVE+VL+SH ++LEAAVV RPD 
Sbjct: 449 GWFRTGDLGVKHEDGYIQLKDRKKDVVISGGENVSTVEVETVLYSHEAVLEAAVVARPDK 508

Query: 481 HWGETPCAFVKLKDG--RSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFIL 540
            WGETPCAFV LK+G     S ++IIKFCR+ L HYMAP+ VVF++LPKTSTGK QK+IL
Sbjct: 509 LWGETPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYIL 568

Query: 541 KEEARAMGSL 547
           KE+A+AMGSL
Sbjct: 569 KEKAKAMGSL 572

BLAST of MC11g0064.1 vs. ExPASy Swiss-Prot
Match: Q8VZF1 (Acetate/butyrate--CoA ligase AAE7, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE7 PE=1 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 1.6e-169
Identity = 290/558 (51.97%), Postives = 391/558 (70.07%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           +D + +  AN+  LTP+ FL+R+A V+  R S+++G   YTWR T  RC RLAS L    
Sbjct: 9   IDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALADRS 68

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           I  G  VA +APN+PAMYE HF VPM GAVL  +NIR NA  V+ LL+HS++ +I+VD +
Sbjct: 69  IGPGSTVAIIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMVDQE 128

Query: 121 FLHVVKGAIKVMFERREK--KAPLVVIIQEFGQGRPS--DSIDPDHLEFESLLATGEVDF 180
           F  + + ++++M E+     K PL+++I +      S   ++    +E+E  LATG+ ++
Sbjct: 129 FFTLAEDSLRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSKGAIEYEDFLATGDPNY 188

Query: 181 KIRRPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMF 240
             + P DEW  I+L YTSGTT+ PKGV+  HRGAY+  LS  L+  M    VYLWT+PMF
Sbjct: 189 PWQPPADEWQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTLPMF 248

Query: 241 HCNGWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQ- 300
           HCNGWCF W +A   GT+IC R VTAKE+++ I+++ VTH   AP VLN I+NAP  +  
Sbjct: 249 HCNGWCFPWSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKEDTI 308

Query: 301 KPLPGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDK 360
            PLP  V VMT GA PP  VL  M   GF V HTYGL+ETYG +TVC+W+PEWDSL  + 
Sbjct: 309 LPLPHTVHVMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLPPET 368

Query: 361 QAKLNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREA 420
           QAKLN+RQG+++ GME++D+ +  T K VPADGKT GE++FRGN VM GYLK+ +A +E 
Sbjct: 369 QAKLNARQGVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEANKET 428

Query: 421 FSGGWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGR 480
           F+GGWF SGD+ V+HP+ YIE+KDRSKD+IISGGENISS+EVE+V++ HP++LEA+VV R
Sbjct: 429 FAGGWFHSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASVVAR 488

Query: 481 PDDHWGETPCAFVKLKD-----GRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGK 540
           PD+ W E+PCAFV LK       ++   ++I+KFCRE L  Y  P+ VVF  LPKT+TGK
Sbjct: 489 PDERWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTATGK 548

Query: 541 TQKFILKEEARAMGSLPK 549
            QK IL+ +A+ MG +P+
Sbjct: 549 IQKHILRTKAKEMGPVPR 566

BLAST of MC11g0064.1 vs. NCBI nr
Match: XP_022132737.1 (probable acyl-activating enzyme 1, peroxisomal [Momordica charantia])

HSP 1 Score: 1124 bits (2907), Expect = 0.0
Identity = 553/553 (100.00%), Postives = 553/553 (100.00%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG
Sbjct: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ
Sbjct: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPDHLEFESLLATGEVDFKIRR 180
           FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPDHLEFESLLATGEVDFKIRR
Sbjct: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPDHLEFESLLATGEVDFKIRR 180

Query: 181 PRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMFHCNG 240
           PRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMFHCNG
Sbjct: 181 PRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMFHCNG 240

Query: 241 WCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQKPLPG 300
           WCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQKPLPG
Sbjct: 241 WCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQKPLPG 300

Query: 301 KVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDKQAKLN 360
           KVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDKQAKLN
Sbjct: 301 KVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDKQAKLN 360

Query: 361 SRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFSGGW 420
           SRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFSGGW
Sbjct: 361 SRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFSGGW 420

Query: 421 FRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGRPDDHW 480
           FRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGRPDDHW
Sbjct: 421 FRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGRPDDHW 480

Query: 481 GETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFILKEEA 540
           GETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFILKEEA
Sbjct: 481 GETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFILKEEA 540

Query: 541 RAMGSLPKRISKL 553
           RAMGSLPKRISKL
Sbjct: 541 RAMGSLPKRISKL 553

BLAST of MC11g0064.1 vs. NCBI nr
Match: XP_022132725.1 (probable acyl-activating enzyme 1, peroxisomal [Momordica charantia])

HSP 1 Score: 975 bits (2520), Expect = 0.0
Identity = 482/558 (86.38%), Postives = 516/558 (92.47%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           MDG +RCSANH PLTPITFLERSAAVY DRISLVYG LRYTW+DTLQRCTRLAS LVRLG
Sbjct: 1   MDGADRCSANHVPLTPITFLERSAAVYGDRISLVYGRLRYTWKDTLQRCTRLASALVRLG 60

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           IA GDVVAALAPN+PAMYELHFAVPMAGAVLCTLNIRH+AAMVSTLLTHSEAKIIIVDYQ
Sbjct: 61  IAPGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNIRHDAAMVSTLLTHSEAKIIIVDYQ 120

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSID-----PDHLEFESLLATGEVD 180
           FLH+VKGAI+VM +R+EK  PLVVIIQE+ Q  PS   D      + LEFE LLATGE+D
Sbjct: 121 FLHIVKGAIEVMSQRKEK-LPLVVIIQEYDQ--PSSHKDRAGSASEDLEFEKLLATGELD 180

Query: 181 FKIRRPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPM 240
           F+IRRP+DEWDPISLNYTSGTTSRPKGVIYSHRGAYLN+LSTVLLN M   P+YLWTVPM
Sbjct: 181 FEIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNSLSTVLLNGMCSNPIYLWTVPM 240

Query: 241 FHCNGWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQ 300
           FHCNGWCFTW VAAQGGTN+CQR VTAKEIF NIS+H VTHM GAPTVLNMIINAPI EQ
Sbjct: 241 FHCNGWCFTWAVAAQGGTNVCQRNVTAKEIFDNISQHKVTHMAGAPTVLNMIINAPISEQ 300

Query: 301 KPLPGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDK 360
           KPLP KVTVMTGGAPPPSHVL KMRA+GFFVVH+YGLTETYG ATVC W+PEWDSL +DK
Sbjct: 301 KPLPEKVTVMTGGAPPPSHVLYKMRALGFFVVHSYGLTETYGPATVCVWKPEWDSLPQDK 360

Query: 361 QAKLNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREA 420
           QAKLNSRQGLQHVGMEE+DIK+P+TM+SVPADGKTMGEVMFRGNTVMNGYLKDLKATREA
Sbjct: 361 QAKLNSRQGLQHVGMEEVDIKDPVTMESVPADGKTMGEVMFRGNTVMNGYLKDLKATREA 420

Query: 421 FSGGWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGR 480
           F+GGWFRSGDLGVRH +GYIELKDRSKDIIISGGENISSIEVESVLFSHP++LEAAVVGR
Sbjct: 421 FNGGWFRSGDLGVRHLDGYIELKDRSKDIIISGGENISSIEVESVLFSHPAVLEAAVVGR 480

Query: 481 PDDHWGETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFI 540
           PDDHWGETPCAFVKLKDG SA+EEEII+FCREHL HYM+PRCVVFKDLPKTSTGKTQKFI
Sbjct: 481 PDDHWGETPCAFVKLKDGCSATEEEIIEFCREHLPHYMSPRCVVFKDLPKTSTGKTQKFI 540

Query: 541 LKEEARAMGSLPKRISKL 553
           LK+EA+AMGSL KRISKL
Sbjct: 541 LKQEAKAMGSLSKRISKL 555

BLAST of MC11g0064.1 vs. NCBI nr
Match: XP_022137950.1 (probable acyl-activating enzyme 1, peroxisomal [Momordica charantia])

HSP 1 Score: 951 bits (2459), Expect = 0.0
Identity = 470/551 (85.30%), Postives = 507/551 (92.01%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           M+GMERCSAN   LTPITFLERSAAVY DR SLVYG +RYTWRDTLQRCTRLAS LVR G
Sbjct: 1   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 60

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           I+RGDVVAALAPN+PAMYELHFAVPMAGAVLCTLN+RH+AAMVSTLL+HSEAKII VDYQ
Sbjct: 61  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 120

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDP-----DHLEFESLLATGEVD 180
           FL +VK AIK+M +  EK  PLVV+IQE+ Q  PS  I+      + LEFESLLATGEVD
Sbjct: 121 FLPMVKRAIKLMSKTTEK-LPLVVVIQEYDQ--PSSCINGVDSALEDLEFESLLATGEVD 180

Query: 181 FKIRRPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPM 240
           FKIRRP+DEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDM  LPVYLWTVPM
Sbjct: 181 FKIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPM 240

Query: 241 FHCNGWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQ 300
           FHCNGWCF+WGVAAQGGTNICQRTVTAKEIFANIS+HNVTHMGGAPTVLNMIINAPI EQ
Sbjct: 241 FHCNGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQ 300

Query: 301 KPLPGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDK 360
           KPLPGKVTVMTGGAPPPSHVL KMRA+GF ++H+YGLTETYG ATVCSW+PEWDSL ED+
Sbjct: 301 KPLPGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQ 360

Query: 361 QAKLNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREA 420
           QAKLNSRQGLQHVG+E +DIK+P+TM+S PADGKTMGEVMFRGNTVM+GYLKDLKAT+EA
Sbjct: 361 QAKLNSRQGLQHVGLEGVDIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEA 420

Query: 421 FSGGWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGR 480
           F+GGWFRSGDLGVRHP+GYIELKDRSKDIIISGGENISSIEVESVL+SHPSILE A+VGR
Sbjct: 421 FNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGR 480

Query: 481 PDDHWGETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFI 540
           PDDHWGETPCAFVKLKDG SA+ EEIIKFCREHL HYMAPR VVF+DLPKTSTGK QKF+
Sbjct: 481 PDDHWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFV 540

Query: 541 LKEEARAMGSL 546
           LKEEA AMGSL
Sbjct: 541 LKEEATAMGSL 548

BLAST of MC11g0064.1 vs. NCBI nr
Match: KAG7024112.1 (putative acyl-activating enzyme 1, peroxisomal [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 944 bits (2439), Expect = 0.0
Identity = 466/558 (83.51%), Postives = 506/558 (90.68%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           MDGM+RCSAN+ PLTPITFLERSAAVY DRISLVYG ++YTWR+TL RCTRLAS LVR+G
Sbjct: 1   MDGMDRCSANYLPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           IARGDVVAALAPN+PAMYELHFAVPMAGAVLC+LN+RH+AAMVSTLL+HSEAKII+VDYQ
Sbjct: 61  IARGDVVAALAPNIPAMYELHFAVPMAGAVLCSLNVRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPD-----HLEFESLLATGEVD 180
           F H+V GAIK M ERREK  PLVVII E+ Q  P   ID +     +LEFESLL +G++D
Sbjct: 121 FEHIVTGAIKAMSERREK-IPLVVIINEYDQ--PPSHIDREGSASGYLEFESLLESGKLD 180

Query: 181 FKIRRPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPM 240
           FKIRRP+DE DPI+LNYTSGTTSRPKGVIYSHRGAYLN LS VLLNDM  LPVYLW VPM
Sbjct: 181 FKIRRPKDELDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWVVPM 240

Query: 241 FHCNGWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQ 300
           FHCNGW  TWGVAAQ GTNICQR  +AKEIF NIS H VTHMGGAPTVLNMIINAP  EQ
Sbjct: 241 FHCNGWSLTWGVAAQSGTNICQRNASAKEIFDNISLHKVTHMGGAPTVLNMIINAPTSEQ 300

Query: 301 KPLPGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDK 360
           KPLPGKVT+MTGGAPPPSHVL KMRAMGF +VH YGLTETYG ATVCSW+PEWDSL +DK
Sbjct: 301 KPLPGKVTMMTGGAPPPSHVLFKMRAMGFLIVHAYGLTETYGPATVCSWKPEWDSLPQDK 360

Query: 361 QAKLNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREA 420
           QAKL+SRQGLQHVG+EE+DIK+P+TM+S PADGKT+GEVM RGNTVM+GYLKDLKATREA
Sbjct: 361 QAKLSSRQGLQHVGLEEVDIKDPVTMESAPADGKTIGEVMVRGNTVMSGYLKDLKATREA 420

Query: 421 FSGGWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGR 480
           F+GGWFRSGDLGVRHP+GYIELKDRSKDIIISGGENISSIEVESVLFSHPS+LEAAVVGR
Sbjct: 421 FNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFSHPSVLEAAVVGR 480

Query: 481 PDDHWGETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFI 540
           PDDHWGETPCAFVKLKDG SASEE+IIKFCREHL HYMAPR VVFKDLPKTSTGKTQKFI
Sbjct: 481 PDDHWGETPCAFVKLKDGCSASEEDIIKFCREHLPHYMAPRSVVFKDLPKTSTGKTQKFI 540

Query: 541 LKEEARAMGSLPKRISKL 553
           LKEEA+AMGSLPK+ SKL
Sbjct: 541 LKEEAKAMGSLPKQTSKL 555

BLAST of MC11g0064.1 vs. NCBI nr
Match: XP_022937090.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 944 bits (2439), Expect = 0.0
Identity = 466/558 (83.51%), Postives = 506/558 (90.68%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           MDGM+RCSAN+ PLTPITFLERSAAVY DRISLVYG ++YTWR+TL RCTRLAS LVR+G
Sbjct: 1   MDGMDRCSANYLPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           IARGDVVAALAPN+PAMYELHFAVPMAGAVLC+LN+RH+AAMVSTLL+HSEAKII+VDYQ
Sbjct: 61  IARGDVVAALAPNIPAMYELHFAVPMAGAVLCSLNVRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPD-----HLEFESLLATGEVD 180
           F H+V GAIK M ERREK  PLVVII E+ Q  P   ID +     +LEFESLL +G++D
Sbjct: 121 FEHIVMGAIKAMSERREK-IPLVVIINEYDQ--PPSHIDREGSASGYLEFESLLESGKLD 180

Query: 181 FKIRRPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPM 240
           FKIRRP+DE DPI+LNYTSGTTSRPKGVIYSHRGAYLN LS VLLNDM  LPVYLW VPM
Sbjct: 181 FKIRRPKDELDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWVVPM 240

Query: 241 FHCNGWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQ 300
           FHCNGW  TWGVAAQ GTNICQR  +AKEIF NIS H VTHMGGAPTVLNMIINAP  EQ
Sbjct: 241 FHCNGWSLTWGVAAQSGTNICQRNASAKEIFDNISLHKVTHMGGAPTVLNMIINAPTSEQ 300

Query: 301 KPLPGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDK 360
           KPLPGKVT+MTGGAPPPSHVL KMRAMGF +VH YGLTETYG ATVCSW+PEWDSL +DK
Sbjct: 301 KPLPGKVTMMTGGAPPPSHVLFKMRAMGFLIVHAYGLTETYGPATVCSWKPEWDSLPQDK 360

Query: 361 QAKLNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREA 420
           QAKL+SRQGLQHVG+EE+DIK+P+TM+S PADGKT+GEVM RGNTVM+GYLKDLKATREA
Sbjct: 361 QAKLSSRQGLQHVGLEEVDIKDPVTMESAPADGKTIGEVMVRGNTVMSGYLKDLKATREA 420

Query: 421 FSGGWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGR 480
           F+GGWFRSGDLGVRHP+GYIELKDRSKDIIISGGENISSIEVESVLFSHPS+LEAAVVGR
Sbjct: 421 FNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFSHPSVLEAAVVGR 480

Query: 481 PDDHWGETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFI 540
           PDDHWGETPCAFVKLKDG SASEE+IIKFCREHL HYMAPR VVFKDLPKTSTGKTQKFI
Sbjct: 481 PDDHWGETPCAFVKLKDGCSASEEDIIKFCREHLPHYMAPRSVVFKDLPKTSTGKTQKFI 540

Query: 541 LKEEARAMGSLPKRISKL 553
           LKEEA+AMGSLPK+ SKL
Sbjct: 541 LKEEAKAMGSLPKQTSKL 555

BLAST of MC11g0064.1 vs. ExPASy TrEMBL
Match: A0A6J1BUN5 (probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111005527 PE=4 SV=1)

HSP 1 Score: 1124 bits (2907), Expect = 0.0
Identity = 553/553 (100.00%), Postives = 553/553 (100.00%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG
Sbjct: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ
Sbjct: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPDHLEFESLLATGEVDFKIRR 180
           FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPDHLEFESLLATGEVDFKIRR
Sbjct: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPDHLEFESLLATGEVDFKIRR 180

Query: 181 PRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMFHCNG 240
           PRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMFHCNG
Sbjct: 181 PRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMFHCNG 240

Query: 241 WCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQKPLPG 300
           WCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQKPLPG
Sbjct: 241 WCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQKPLPG 300

Query: 301 KVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDKQAKLN 360
           KVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDKQAKLN
Sbjct: 301 KVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDKQAKLN 360

Query: 361 SRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFSGGW 420
           SRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFSGGW
Sbjct: 361 SRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFSGGW 420

Query: 421 FRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGRPDDHW 480
           FRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGRPDDHW
Sbjct: 421 FRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGRPDDHW 480

Query: 481 GETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFILKEEA 540
           GETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFILKEEA
Sbjct: 481 GETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFILKEEA 540

Query: 541 RAMGSLPKRISKL 553
           RAMGSLPKRISKL
Sbjct: 541 RAMGSLPKRISKL 553

BLAST of MC11g0064.1 vs. ExPASy TrEMBL
Match: A0A6J1BTA0 (probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111005519 PE=4 SV=1)

HSP 1 Score: 975 bits (2520), Expect = 0.0
Identity = 482/558 (86.38%), Postives = 516/558 (92.47%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           MDG +RCSANH PLTPITFLERSAAVY DRISLVYG LRYTW+DTLQRCTRLAS LVRLG
Sbjct: 1   MDGADRCSANHVPLTPITFLERSAAVYGDRISLVYGRLRYTWKDTLQRCTRLASALVRLG 60

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           IA GDVVAALAPN+PAMYELHFAVPMAGAVLCTLNIRH+AAMVSTLLTHSEAKIIIVDYQ
Sbjct: 61  IAPGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNIRHDAAMVSTLLTHSEAKIIIVDYQ 120

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSID-----PDHLEFESLLATGEVD 180
           FLH+VKGAI+VM +R+EK  PLVVIIQE+ Q  PS   D      + LEFE LLATGE+D
Sbjct: 121 FLHIVKGAIEVMSQRKEK-LPLVVIIQEYDQ--PSSHKDRAGSASEDLEFEKLLATGELD 180

Query: 181 FKIRRPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPM 240
           F+IRRP+DEWDPISLNYTSGTTSRPKGVIYSHRGAYLN+LSTVLLN M   P+YLWTVPM
Sbjct: 181 FEIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNSLSTVLLNGMCSNPIYLWTVPM 240

Query: 241 FHCNGWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQ 300
           FHCNGWCFTW VAAQGGTN+CQR VTAKEIF NIS+H VTHM GAPTVLNMIINAPI EQ
Sbjct: 241 FHCNGWCFTWAVAAQGGTNVCQRNVTAKEIFDNISQHKVTHMAGAPTVLNMIINAPISEQ 300

Query: 301 KPLPGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDK 360
           KPLP KVTVMTGGAPPPSHVL KMRA+GFFVVH+YGLTETYG ATVC W+PEWDSL +DK
Sbjct: 301 KPLPEKVTVMTGGAPPPSHVLYKMRALGFFVVHSYGLTETYGPATVCVWKPEWDSLPQDK 360

Query: 361 QAKLNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREA 420
           QAKLNSRQGLQHVGMEE+DIK+P+TM+SVPADGKTMGEVMFRGNTVMNGYLKDLKATREA
Sbjct: 361 QAKLNSRQGLQHVGMEEVDIKDPVTMESVPADGKTMGEVMFRGNTVMNGYLKDLKATREA 420

Query: 421 FSGGWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGR 480
           F+GGWFRSGDLGVRH +GYIELKDRSKDIIISGGENISSIEVESVLFSHP++LEAAVVGR
Sbjct: 421 FNGGWFRSGDLGVRHLDGYIELKDRSKDIIISGGENISSIEVESVLFSHPAVLEAAVVGR 480

Query: 481 PDDHWGETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFI 540
           PDDHWGETPCAFVKLKDG SA+EEEII+FCREHL HYM+PRCVVFKDLPKTSTGKTQKFI
Sbjct: 481 PDDHWGETPCAFVKLKDGCSATEEEIIEFCREHLPHYMSPRCVVFKDLPKTSTGKTQKFI 540

Query: 541 LKEEARAMGSLPKRISKL 553
           LK+EA+AMGSL KRISKL
Sbjct: 541 LKQEAKAMGSLSKRISKL 555

BLAST of MC11g0064.1 vs. ExPASy TrEMBL
Match: A0A6J1C834 (probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111009236 PE=4 SV=1)

HSP 1 Score: 951 bits (2459), Expect = 0.0
Identity = 470/551 (85.30%), Postives = 507/551 (92.01%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           M+GMERCSAN   LTPITFLERSAAVY DR SLVYG +RYTWRDTLQRCTRLAS LVR G
Sbjct: 1   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 60

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           I+RGDVVAALAPN+PAMYELHFAVPMAGAVLCTLN+RH+AAMVSTLL+HSEAKII VDYQ
Sbjct: 61  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 120

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDP-----DHLEFESLLATGEVD 180
           FL +VK AIK+M +  EK  PLVV+IQE+ Q  PS  I+      + LEFESLLATGEVD
Sbjct: 121 FLPMVKRAIKLMSKTTEK-LPLVVVIQEYDQ--PSSCINGVDSALEDLEFESLLATGEVD 180

Query: 181 FKIRRPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPM 240
           FKIRRP+DEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDM  LPVYLWTVPM
Sbjct: 181 FKIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPM 240

Query: 241 FHCNGWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQ 300
           FHCNGWCF+WGVAAQGGTNICQRTVTAKEIFANIS+HNVTHMGGAPTVLNMIINAPI EQ
Sbjct: 241 FHCNGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQ 300

Query: 301 KPLPGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDK 360
           KPLPGKVTVMTGGAPPPSHVL KMRA+GF ++H+YGLTETYG ATVCSW+PEWDSL ED+
Sbjct: 301 KPLPGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQ 360

Query: 361 QAKLNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREA 420
           QAKLNSRQGLQHVG+E +DIK+P+TM+S PADGKTMGEVMFRGNTVM+GYLKDLKAT+EA
Sbjct: 361 QAKLNSRQGLQHVGLEGVDIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEA 420

Query: 421 FSGGWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGR 480
           F+GGWFRSGDLGVRHP+GYIELKDRSKDIIISGGENISSIEVESVL+SHPSILE A+VGR
Sbjct: 421 FNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGR 480

Query: 481 PDDHWGETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFI 540
           PDDHWGETPCAFVKLKDG SA+ EEIIKFCREHL HYMAPR VVF+DLPKTSTGK QKF+
Sbjct: 481 PDDHWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFV 540

Query: 541 LKEEARAMGSL 546
           LKEEA AMGSL
Sbjct: 541 LKEEATAMGSL 548

BLAST of MC11g0064.1 vs. ExPASy TrEMBL
Match: A0A6J1FFJ8 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111443496 PE=4 SV=1)

HSP 1 Score: 944 bits (2439), Expect = 0.0
Identity = 466/558 (83.51%), Postives = 506/558 (90.68%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           MDGM+RCSAN+ PLTPITFLERSAAVY DRISLVYG ++YTWR+TL RCTRLAS LVR+G
Sbjct: 1   MDGMDRCSANYLPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           IARGDVVAALAPN+PAMYELHFAVPMAGAVLC+LN+RH+AAMVSTLL+HSEAKII+VDYQ
Sbjct: 61  IARGDVVAALAPNIPAMYELHFAVPMAGAVLCSLNVRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPD-----HLEFESLLATGEVD 180
           F H+V GAIK M ERREK  PLVVII E+ Q  P   ID +     +LEFESLL +G++D
Sbjct: 121 FEHIVMGAIKAMSERREK-IPLVVIINEYDQ--PPSHIDREGSASGYLEFESLLESGKLD 180

Query: 181 FKIRRPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPM 240
           FKIRRP+DE DPI+LNYTSGTTSRPKGVIYSHRGAYLN LS VLLNDM  LPVYLW VPM
Sbjct: 181 FKIRRPKDELDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWVVPM 240

Query: 241 FHCNGWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQ 300
           FHCNGW  TWGVAAQ GTNICQR  +AKEIF NIS H VTHMGGAPTVLNMIINAP  EQ
Sbjct: 241 FHCNGWSLTWGVAAQSGTNICQRNASAKEIFDNISLHKVTHMGGAPTVLNMIINAPTSEQ 300

Query: 301 KPLPGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDK 360
           KPLPGKVT+MTGGAPPPSHVL KMRAMGF +VH YGLTETYG ATVCSW+PEWDSL +DK
Sbjct: 301 KPLPGKVTMMTGGAPPPSHVLFKMRAMGFLIVHAYGLTETYGPATVCSWKPEWDSLPQDK 360

Query: 361 QAKLNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREA 420
           QAKL+SRQGLQHVG+EE+DIK+P+TM+S PADGKT+GEVM RGNTVM+GYLKDLKATREA
Sbjct: 361 QAKLSSRQGLQHVGLEEVDIKDPVTMESAPADGKTIGEVMVRGNTVMSGYLKDLKATREA 420

Query: 421 FSGGWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGR 480
           F+GGWFRSGDLGVRHP+GYIELKDRSKDIIISGGENISSIEVESVLFSHPS+LEAAVVGR
Sbjct: 421 FNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFSHPSVLEAAVVGR 480

Query: 481 PDDHWGETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFI 540
           PDDHWGETPCAFVKLKDG SASEE+IIKFCREHL HYMAPR VVFKDLPKTSTGKTQKFI
Sbjct: 481 PDDHWGETPCAFVKLKDGCSASEEDIIKFCREHLPHYMAPRSVVFKDLPKTSTGKTQKFI 540

Query: 541 LKEEARAMGSLPKRISKL 553
           LKEEA+AMGSLPK+ SKL
Sbjct: 541 LKEEAKAMGSLPKQTSKL 555

BLAST of MC11g0064.1 vs. ExPASy TrEMBL
Match: A0A6J1IF13 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111473618 PE=4 SV=1)

HSP 1 Score: 934 bits (2415), Expect = 0.0
Identity = 463/558 (82.97%), Postives = 502/558 (89.96%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           MDGM+RCSAN+ PLTPITFLERSAAVY DRISL YG ++YTWR+TL RCTRLAS LVR+G
Sbjct: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLAYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           IARGDVVAALAPN+PAMYELHFAVPMAGAVLC+LN+RH+AAMVSTLL+HSEAKII+VDYQ
Sbjct: 61  IARGDVVAALAPNIPAMYELHFAVPMAGAVLCSLNVRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSID-----PDHLEFESLLATGEVD 180
           F H+V GAIK M ERREK  PLVVII E+ Q  P   ID      ++LEFESLL +G++D
Sbjct: 121 FEHIVMGAIKAMSERREK-IPLVVIINEYDQ--PPSYIDREGSASEYLEFESLLESGKLD 180

Query: 181 FKIRRPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPM 240
           FKIRRP+ E DPI+LNYTSGTTSRPKGVIYSHRGAYLN LS VLLNDM  LPVYLW VPM
Sbjct: 181 FKIRRPKYELDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWVVPM 240

Query: 241 FHCNGWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQ 300
           FHCNGW  TWGVAAQ GTNICQR V+AKEIF NIS H VTHMGGAPTVLNMIINAP  EQ
Sbjct: 241 FHCNGWSLTWGVAAQSGTNICQRNVSAKEIFENISLHKVTHMGGAPTVLNMIINAPTSEQ 300

Query: 301 KPLPGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDK 360
           KPLP KVT+MTGGAPPPSHVL KMRAMGF +VH YGLTETYG ATVCSW+PEWDSL +DK
Sbjct: 301 KPLPRKVTMMTGGAPPPSHVLFKMRAMGFLIVHAYGLTETYGPATVCSWKPEWDSLPQDK 360

Query: 361 QAKLNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREA 420
           QAKL SRQGLQHVG+EE+DIK+P+TM+S PADGKT+GEVM RGNTVM+GYLKDLKATREA
Sbjct: 361 QAKLTSRQGLQHVGLEEVDIKDPVTMESAPADGKTIGEVMVRGNTVMSGYLKDLKATREA 420

Query: 421 FSGGWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGR 480
           F+GGWFRSGDLGVRHP+GYIELKDRSKDIIISGGENISSIEVESVLFSHPS+LEAAVVGR
Sbjct: 421 FNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFSHPSVLEAAVVGR 480

Query: 481 PDDHWGETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFI 540
           PDD WGETPCAFVKLKDG SASEE+IIKFCREHL HYMAPR VVFKDLPKTSTGKTQKFI
Sbjct: 481 PDDRWGETPCAFVKLKDGCSASEEDIIKFCREHLPHYMAPRSVVFKDLPKTSTGKTQKFI 540

Query: 541 LKEEARAMGSLPKRISKL 553
           LKEEA+AMGSLPK+ SKL
Sbjct: 541 LKEEAKAMGSLPKQTSKL 555

BLAST of MC11g0064.1 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 768.5 bits (1983), Expect = 3.8e-222
Identity = 355/549 (64.66%), Postives = 445/549 (81.06%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           M+G  +  AN+ PLTPI+FL+RSA VYADR+S+VYG ++YTWR T  RC R+AS L +LG
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           I+ GDVV+ LAPNVPAM ELHF VPMAGA+LCTLNIRH++++V+ LL HS  K+I  D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPDHLEFESLLATGEVDFKIRR 180
           FL + +GA +++  + +K   LV+I +   Q         + +E+E ++A G+ DF++ R
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 PRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMFHCNG 240
           P DE D IS+NYTSGTTS PKGV+YSHRGAYLN+L+ VLLN+M   P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQKPLPG 300
           WC  WGV A GGTNIC R VTAK IF NIS+H VTHMGGAPT+LNMIINAP  EQKPLPG
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 KVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDKQAKLN 360
           KV+ +TG APPP+HV+ KM  +GF + H+YGLTETYG  T+C+W+PEWDSL  ++QAK+ 
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 SRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFSGGW 420
           +RQG+ H+G+EE+ +K+P+TM+++PADG TMGEV+FRGNTVMNGYLK+ +AT+EAF GGW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGRPDDHW 480
           F SGDLGV+HP+GYIELKDRSKDIIISGGENISSIEVES LF+HP +LEAAVV RPD++W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFILKEEA 540
           GET CAFVKLKDG  AS EE+I +CR+ L HYMAPR +VF+DLPKTSTGK QKF+L+ +A
Sbjct: 483 GETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKA 542

Query: 541 RAMGSLPKR 550
           +A+ SL K+
Sbjct: 543 KALVSLSKK 551

BLAST of MC11g0064.1 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 726.5 bits (1874), Expect = 1.6e-209
Identity = 345/558 (61.83%), Postives = 431/558 (77.24%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           ++G+ R  AN +PL+PITFLERSA VY DR SLV+G +++TW  T QRC RLAS L  LG
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           I+RGDVVAALAPNVPAM+ELHFAVPMAG +LC LN R + + +S LL HSEAKI+ VD+Q
Sbjct: 108 ISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQ 167

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPD-----------HLEFESLL 180
            L +  GA+ ++ +  + +  L +++    Q    D  D D             E+E+LL
Sbjct: 168 LLEIAHGALDLLAKSDKTRKSLKLVL--ISQSNDDDDSDEDSSSTFASKYSFDYEYETLL 227

Query: 181 ATGEVDFKIRRPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVY 240
            +G+ +F+I +PR EWDPIS+NYTSGTTSRPKGV+YSHRGAYLN+L+TV L+ MS  PVY
Sbjct: 228 KSGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVY 287

Query: 241 LWTVPMFHCNGWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIIN 300
           LWTVPMFHCNGWC  WGVAAQGGTNIC R V+ K IF NI+ H VTHMGGAPTVLNMI+N
Sbjct: 288 LWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVN 347

Query: 301 APIIEQKPLPGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWD 360
             + E KPLP +V +MTGG+PP   +L KM  +GF V H YGLTETYG  T C W+PEWD
Sbjct: 348 YTVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWD 407

Query: 361 SLHEDKQAKLNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDL 420
           SL  +++ KL +RQG+QH+G+E +D+K+P+TM++VP DG TMGEVMFRGNTVM+GY KD+
Sbjct: 408 SLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDI 467

Query: 421 KATREAFSGGWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILE 480
           +ATR+AF G WF SGDL V++P+GYIE+KDR KD+IISGGENISS+EVE VL SH ++LE
Sbjct: 468 EATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLE 527

Query: 481 AAVVGRPDDHWGETPCAFVKLKDG-RSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTST 540
           AAVV RPD HWG+TPC FVKLK+G  +   EEII FCR+HL HYMAP+ +VF D+PKTST
Sbjct: 528 AAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTST 587

Query: 541 GKTQKFILKEEARAMGSL 547
           GK QK++L+++A  MGSL
Sbjct: 588 GKVQKYLLRKKADEMGSL 603

BLAST of MC11g0064.1 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 667.2 bits (1720), Expect = 1.2e-191
Identity = 307/473 (64.90%), Postives = 384/473 (81.18%), Query Frame = 0

Query: 77  MYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQFLHVVKGAIKVMFERR 136
           M ELHF VPMAGA+LCTLNIRH++++V+ LL HS  K+I  D+QFL + +GA +++  + 
Sbjct: 1   MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60

Query: 137 EKKAPLVVIIQEFGQGRPSDSIDPDHLEFESLLATGEVDFKIRRPRDEWDPISLNYTSGT 196
           +K   LV+I +   Q         + +E+E ++A G+ DF++ RP DE D IS+NYTSGT
Sbjct: 61  DKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTSGT 120

Query: 197 TSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMFHCNGWCFTWGVAAQGGTNIC 256
           TS PKGV+YSHRGAYLN+L+ VLLN+M   P YLWT PMFHCNGWC  WGV A GGTNIC
Sbjct: 121 TSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNGWCLLWGVTAIGGTNIC 180

Query: 257 QRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQKPLPGKVTVMTGGAPPPSHVL 316
            R VTAK IF NIS+H VTHMGGAPT+LNMIINAP  EQKPLPGKV+ +TG APPP+HV+
Sbjct: 181 LRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGAAPPPAHVI 240

Query: 317 CKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDKQAKLNSRQGLQHVGMEEMDIK 376
            KM  +GF + H+YGLTETYG  T+C+W+PEWDSL  ++QAK+ +RQG+ H+G+EE+ +K
Sbjct: 241 FKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGLEEIQVK 300

Query: 377 NPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFSGGWFRSGDLGVRHPNGYIE 436
           +P+TM+++PADG TMGEV+FRGNTVMNGYLK+ +AT+EAF GGWF SGDLGV+HP+GYIE
Sbjct: 301 DPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKHPDGYIE 360

Query: 437 LKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGRPDDHWGETPCAFVKLKDGRSA 496
           LKDRSKDIIISGGENISSIEVES LF+HP +LEAAVV RPD++WGET CAFVKLKDG  A
Sbjct: 361 LKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDGSKA 420

Query: 497 SEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFILKEEARAMGSLPKR 550
           S EE+I +CR+ L HYMAPR +VF+DLPKTSTGK QKF+L+ +A+A+ SL K+
Sbjct: 421 SAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKALVSLSKK 473

BLAST of MC11g0064.1 vs. TAIR 10
Match: AT3G16910.1 (acyl-activating enzyme 7 )

HSP 1 Score: 597.4 bits (1539), Expect = 1.1e-170
Identity = 290/558 (51.97%), Postives = 391/558 (70.07%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           +D + +  AN+  LTP+ FL+R+A V+  R S+++G   YTWR T  RC RLAS L    
Sbjct: 9   IDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALADRS 68

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           I  G  VA +APN+PAMYE HF VPM GAVL  +NIR NA  V+ LL+HS++ +I+VD +
Sbjct: 69  IGPGSTVAIIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMVDQE 128

Query: 121 FLHVVKGAIKVMFERREK--KAPLVVIIQEFGQGRPS--DSIDPDHLEFESLLATGEVDF 180
           F  + + ++++M E+     K PL+++I +      S   ++    +E+E  LATG+ ++
Sbjct: 129 FFTLAEDSLRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSKGAIEYEDFLATGDPNY 188

Query: 181 KIRRPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMF 240
             + P DEW  I+L YTSGTT+ PKGV+  HRGAY+  LS  L+  M    VYLWT+PMF
Sbjct: 189 PWQPPADEWQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTLPMF 248

Query: 241 HCNGWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQ- 300
           HCNGWCF W +A   GT+IC R VTAKE+++ I+++ VTH   AP VLN I+NAP  +  
Sbjct: 249 HCNGWCFPWSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKEDTI 308

Query: 301 KPLPGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDK 360
            PLP  V VMT GA PP  VL  M   GF V HTYGL+ETYG +TVC+W+PEWDSL  + 
Sbjct: 309 LPLPHTVHVMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLPPET 368

Query: 361 QAKLNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREA 420
           QAKLN+RQG+++ GME++D+ +  T K VPADGKT GE++FRGN VM GYLK+ +A +E 
Sbjct: 369 QAKLNARQGVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEANKET 428

Query: 421 FSGGWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGR 480
           F+GGWF SGD+ V+HP+ YIE+KDRSKD+IISGGENISS+EVE+V++ HP++LEA+VV R
Sbjct: 429 FAGGWFHSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASVVAR 488

Query: 481 PDDHWGETPCAFVKLKD-----GRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGK 540
           PD+ W E+PCAFV LK       ++   ++I+KFCRE L  Y  P+ VVF  LPKT+TGK
Sbjct: 489 PDERWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTATGK 548

Query: 541 TQKFILKEEARAMGSLPK 549
            QK IL+ +A+ MG +P+
Sbjct: 549 IQKHILRTKAKEMGPVPR 566

BLAST of MC11g0064.1 vs. TAIR 10
Match: AT1G76290.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 566.6 bits (1459), Expect = 2.2e-161
Identity = 279/552 (50.54%), Postives = 380/552 (68.84%), Query Frame = 0

Query: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60
           M+G  RCSAN+ PL+PI+FLER+A V+  R S+VYG ++YTW  T  RC RLAS L  LG
Sbjct: 1   MEGTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASALSDLG 60

Query: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120
           ++R DVVAALAPNVPA+ EL+F  PMAGAVLC LN   ++ M++  L  ++ K+  VD +
Sbjct: 61  LSRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDSE 120

Query: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDPDHLEFESLLATGEVDFKIRR 180
           FL V + ++ ++    EK  PL++ I E     P++     + ++E  L+TG  +FK  R
Sbjct: 121 FLSVAEESLSLLSNIEEK--PLIITITE----NPTE--QSKYEQYEDFLSTGNPNFKPIR 180

Query: 181 PRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPMFHCNG 240
           P DE DPI+LN+TSGTTS PK V+YSHRGAYLN  +  ++N+M P+PVYL TVPM+HC+G
Sbjct: 181 PVDECDPIALNFTSGTTSTPKCVVYSHRGAYLNATAVGVMNEMKPMPVYLCTVPMYHCSG 240

Query: 241 WCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQKPLPG 300
           WC+ W V A GG  +C R V  + IF +I +H VT+ GG+P VLNMI NA    +K  P 
Sbjct: 241 WCYIWTVTAFGGVIVCLREVNDEVIFDSIVKHKVTNFGGSPPVLNMIANARDSVKKSFPW 300

Query: 301 KVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDKQAKLN 360
            V VM+GG+ PP  V+ K++ +GF V+  YG +E YG  T C W PEW++L E++  +L 
Sbjct: 301 TVQVMSGGSSPP-EVMLKLKKLGFKVMMAYGCSEVYGLGTACLWMPEWETLPEEESLRLK 360

Query: 361 SRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFSGGW 420
           +R GL H   E +D+ +P TMKSVP DGKT+  +  RGNTVM+GY KD +AT  AF GGW
Sbjct: 361 ARDGLNHFAKEAVDVLDPTTMKSVPHDGKTIRVIALRGNTVMSGYFKDKEATEAAFRGGW 420

Query: 421 FRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGRPDDHW 480
           + S D+GV  P+GYI+ KDRS+D+I  GGE + S E+E +L+SHP++ +A VVGRPD+  
Sbjct: 421 YWSRDMGVIDPDGYIQFKDRSQDVITCGGEIVGSKEIEGILYSHPAVYDAGVVGRPDETL 480

Query: 481 GETPCAFVKLKDGRSASEEEIIKFCREHLSH---YMAPRCVVFKDLPKTSTGKTQKFILK 540
           GE+ CAFVKLK+G  A EEEII+FC+  L +    M P+ VVF D+PKT TGK +K +L+
Sbjct: 481 GESMCAFVKLKEGAEAREEEIIEFCKRKLGNKNMKMIPKTVVFSDVPKTPTGKIRKNVLR 540

Query: 541 EEARAMGSLPKR 550
           + A+ MG +  R
Sbjct: 541 KMAKDMGYVQLR 543