MC10g0432.1 (mRNA) Bitter gourd (Dali-11) v1

Overview
NameMC10g0432.1
TypemRNA
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionnigrin b-like
LocationMC10: 3480898 .. 3481761 (-)
Sequence length864
RNA-Seq ExpressionMC10g0432.1
SyntenyMC10g0432.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGGGATGGTGGTCTACATTGTTGTCGCACTTTCAGTACTAATGAGTGTCTATCGTGGCATGGCGGCCTATCCATATCCTGGGTATGGAAGCTTCGGAAAAATATCACATATTGTAGGTCGAGATGGAATGTGTTTGGACATTGTTGTTCCCAATGTCCGACCACTTTACGCTCAAACTCAATTATCGCGGTGTGACGTTGCGAAACAGACCCAAATGTGGAGCATCCTTGAAGATGGCACCATTCGAACCAAGGACAACTTATATTGCTTGGTTCCTTACAACGATGCGTATGAAGCTAAAGCCGTATTGGGCAAGTGCTCTGAATTGATAAGAAGTGACAAGAAATGGAAGCATAGGAGTGATGGCACTTTTGTCCACGAGAACTCGGGCTTCGTCCTGACAGCGAAACCAGACAACTTCGTGATATTGGGAACTGACGCATACACCCCATCCCAAAGCTGGGAAGCCACCGAGGGTCTCTCCCCTTCTGTGGTTAACATCAAGTGGCTTGAAAACCAGTGTTTGCAGTCCATGGATAATGGTGCTCCCTTTGTGTATTTGAATCTATGTGATAAAAAAAATAAGAACCAACATTGGGCACTATATTCTGACGGCACCATCCGTGTTGATAACTATGGCAGCAGAGACTACTGCTTGACGTCTCAAAGAAATCCATCTAGTGTCATCCTTGTGTCTAACTGTGACGACAAGGCGACACAACGATGGGATATTAATGAAGACAACTTCATTTACCATCCCAACACTAACTTGGTCTTGGATGTCGATAGAAAACCTGAGGGATCCCAAATTGTCGTTATCGACACTCGTGATGGCACTCCTACCCAGCAATGGACGATA

mRNA sequence

ATGAGAGGGATGGTGGTCTACATTGTTGTCGCACTTTCAGTACTAATGAGTGTCTATCGTGGCATGGCGGCCTATCCATATCCTGGGTATGGAAGCTTCGGAAAAATATCACATATTGTAGGTCGAGATGGAATGTGTTTGGACATTGTTGTTCCCAATGTCCGACCACTTTACGCTCAAACTCAATTATCGCGGTGTGACGTTGCGAAACAGACCCAAATGTGGAGCATCCTTGAAGATGGCACCATTCGAACCAAGGACAACTTATATTGCTTGGTTCCTTACAACGATGCGTATGAAGCTAAAGCCGTATTGGGCAAGTGCTCTGAATTGATAAGAAGTGACAAGAAATGGAAGCATAGGAGTGATGGCACTTTTGTCCACGAGAACTCGGGCTTCGTCCTGACAGCGAAACCAGACAACTTCGTGATATTGGGAACTGACGCATACACCCCATCCCAAAGCTGGGAAGCCACCGAGGGTCTCTCCCCTTCTGTGGTTAACATCAAGTGGCTTGAAAACCAGTGTTTGCAGTCCATGGATAATGGTGCTCCCTTTGTGTATTTGAATCTATGTGATAAAAAAAATAAGAACCAACATTGGGCACTATATTCTGACGGCACCATCCGTGTTGATAACTATGGCAGCAGAGACTACTGCTTGACGTCTCAAAGAAATCCATCTAGTGTCATCCTTGTGTCTAACTGTGACGACAAGGCGACACAACGATGGGATATTAATGAAGACAACTTCATTTACCATCCCAACACTAACTTGGTCTTGGATGTCGATAGAAAACCTGAGGGATCCCAAATTGTCGTTATCGACACTCGTGATGGCACTCCTACCCAGCAATGGACGATA

Coding sequence (CDS)

ATGAGAGGGATGGTGGTCTACATTGTTGTCGCACTTTCAGTACTAATGAGTGTCTATCGTGGCATGGCGGCCTATCCATATCCTGGGTATGGAAGCTTCGGAAAAATATCACATATTGTAGGTCGAGATGGAATGTGTTTGGACATTGTTGTTCCCAATGTCCGACCACTTTACGCTCAAACTCAATTATCGCGGTGTGACGTTGCGAAACAGACCCAAATGTGGAGCATCCTTGAAGATGGCACCATTCGAACCAAGGACAACTTATATTGCTTGGTTCCTTACAACGATGCGTATGAAGCTAAAGCCGTATTGGGCAAGTGCTCTGAATTGATAAGAAGTGACAAGAAATGGAAGCATAGGAGTGATGGCACTTTTGTCCACGAGAACTCGGGCTTCGTCCTGACAGCGAAACCAGACAACTTCGTGATATTGGGAACTGACGCATACACCCCATCCCAAAGCTGGGAAGCCACCGAGGGTCTCTCCCCTTCTGTGGTTAACATCAAGTGGCTTGAAAACCAGTGTTTGCAGTCCATGGATAATGGTGCTCCCTTTGTGTATTTGAATCTATGTGATAAAAAAAATAAGAACCAACATTGGGCACTATATTCTGACGGCACCATCCGTGTTGATAACTATGGCAGCAGAGACTACTGCTTGACGTCTCAAAGAAATCCATCTAGTGTCATCCTTGTGTCTAACTGTGACGACAAGGCGACACAACGATGGGATATTAATGAAGACAACTTCATTTACCATCCCAACACTAACTTGGTCTTGGATGTCGATAGAAAACCTGAGGGATCCCAAATTGTCGTTATCGACACTCGTGATGGCACTCCTACCCAGCAATGGACGATA

Protein sequence

MRGMVVYIVVALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQTQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDAYEAKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGSRDYCLTSQRNPSSVILVSNCDDKATQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTI
Homology
[Close]
BLAST of MC10g0432.1 vs. ExPASy Swiss-Prot
Match: P82683 (Beta-galactoside-specific lectin 3 OS=Viscum album OX=3972 PE=1 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 1.8e-26
Identity = 81/257 (31.52%), Postives = 124/257 (48.25%), Query Frame = 0 

Query: 39  IVGRDGMCLDI---VVPNVRPLYAQTQLSRC-DVAKQTQMWSILEDGTIRTKDNLYCLVP 98
           IVGRDG+C+D+      N  P+    QLS C       Q+W+I  DGTIR+  N  CL  
Sbjct: 320 IVGRDGLCVDVRDGKFHNGNPI----QLSPCKSNTDPNQLWTIRRDGTIRS--NGRCLTT 379

Query: 99  YNDAYEAKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTA---KPDNFVILGTDAYT 158
           Y        ++  C+  +R    W+   +GT ++  S  VL A        + + T  Y+
Sbjct: 380 YGYTAGVYVMIFDCNTAVREATLWQIWGNGTIINPRSNLVLGAASGSSGTTLTVQTQVYS 439

Query: 159 PSQSWEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIRV 218
             Q W A    +P  V I    + C+++  NGA  V++  C    +NQ+WALY DG+IR 
Sbjct: 440 LGQGWLAGNDTAPREVTIYGFRDLCMEA--NGAS-VWVETCGSSTENQNWALYGDGSIRP 499

Query: 219 DNYGSRDYCLTSQRNP-SSVILVSNCD-DKATQRWDINEDNFIYHPNTNLVLDVDRKPEG 278
               ++D CLT Q +  ++VI + +C    + QRW    +  I + N  LV+DV +    
Sbjct: 500 KQ--NQDQCLTCQGDSVATVINIVSCSAGSSGQRWVFTNEGTILNLNNGLVMDVAQSNPS 559

Query: 279 SQIVVIDTRDGTPTQQW 287
            + ++I    G P Q W
Sbjct: 560 LRRIIIYPATGNPNQMW 565

[Close]
BLAST of MC10g0432.1 vs. ExPASy Swiss-Prot
Match: P28590 (Abrin-c OS=Abrus precatorius OX=3816 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.5e-25
Identity = 72/253 (28.46%), Postives = 122/253 (48.22%), Query Frame = 0 

Query: 39  IVGRDGMCLDIVVPNVRPLYAQTQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDA 98
           I GRDGMC+D+                 D  ++ Q+W++  D TIR+  N  CL     A
Sbjct: 313 IGGRDGMCVDVYDDGYHNGNRIIAWKCKDRLEENQLWTLKSDKTIRS--NGKCLTTEGYA 372

Query: 99  YEAKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTAKPDNF---VILGTDAYTPSQS 158
                ++  C+  +     W+   +GT ++  S  VL+A+  +    + + T+ Y   Q 
Sbjct: 373 PGNYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMRQG 432

Query: 159 WEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYG 218
           W      SP V +I    + C+Q+  +    V+L  CD   K Q WALY+DG+IR  +  
Sbjct: 433 WRTGNNTSPFVTSISGYSDLCMQAQGSN---VWLADCDNNKKEQQWALYTDGSIR--SVQ 492

Query: 219 SRDYCLTSQ--RNPSSVILVSNCDDKATQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIV 278
           + + CLTS+  +  S ++L++  +  A+QRW    D  IY+ + ++V+DV R     + +
Sbjct: 493 NTNNCLTSKDHKQGSPIVLMACSNGWASQRWLFKNDGSIYNLHDDMVMDVKRSDPSLKEI 552

Query: 279 VIDTRDGTPTQQW 287
           ++    G P Q W
Sbjct: 553 ILHPYHGKPNQIW 558

[Close]
BLAST of MC10g0432.1 vs. ExPASy Swiss-Prot
Match: Q06077 (Abrin-b OS=Abrus precatorius OX=3816 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.5e-25
Identity = 71/253 (28.06%), Postives = 122/253 (48.22%), Query Frame = 0 

Query: 39  IVGRDGMCLDIVVPNVRPLYAQTQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDA 98
           I GR+GMC+D+                 D  ++ Q+W++  D TIR+  N  CL     A
Sbjct: 278 IGGRNGMCVDVYDDGYHNGNRIIAWKCKDRLEENQLWTLKSDKTIRS--NGKCLTTEGYA 337

Query: 99  YEAKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTAKPDNF---VILGTDAYTPSQS 158
                ++  C+  +     W+   +GT ++  S  VL+A+  +    + + T+ Y   Q 
Sbjct: 338 PGNYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMRQG 397

Query: 159 WEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYG 218
           W      SP V +I    + C+Q+  +    V+L  CD   K Q WALY+DG+IR  +  
Sbjct: 398 WRTGNNTSPFVTSISGYSDLCMQAQGSN---VWLAYCDNNKKEQQWALYTDGSIR--SVQ 457

Query: 219 SRDYCLTSQ--RNPSSVILVSNCDDKATQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIV 278
           + + CLTS+  +  S ++L++  +  A+QRW    D  IY+ + ++V+DV R     + +
Sbjct: 458 NTNNCLTSKDHKQGSPIVLMACSNGWASQRWLFRNDGSIYNLHDDMVMDVKRSDPSLKEI 517

Query: 279 VIDTRDGTPTQQW 287
           ++    G P Q W
Sbjct: 518 ILHPYHGKPNQIW 523

[Close]
BLAST of MC10g0432.1 vs. ExPASy Swiss-Prot
Match: Q9M654 (Ribosome-inactivating protein PMRIPm OS=Polygonatum multiflorum OX=45371 GN=RIPm PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.5e-25
Identity = 76/257 (29.57%), Postives = 115/257 (44.75%), Query Frame = 0 

Query: 39  IVGRDGMCLDIVVPNVRPLYAQTQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDA 98
           I GR G C+D+             LS  D  ++ Q W+   DGTIR+K    C+  Y   
Sbjct: 341 ISGRAGWCVDVKDGRDNDGNPIQVLSCGDGQERKQQWTFHRDGTIRSKLG-KCMTAYGFK 400

Query: 99  YEAKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTA---KPDNFVILGTDAYTPSQS 158
           +    ++  C   I    KW    DGT  +  SG VLTA        +++  + +   Q 
Sbjct: 401 HGEYVMIYDCDTAIAGANKWVVSIDGTITNPISGLVLTAPRGATGTTLLVEKNVHAARQC 460

Query: 159 WEATEGLSPSVVNIKWLENQCLQS--MDN----GAPFVYLNLCDKKNKNQHWALYSDGTI 218
           W   + + P V  I   + +CL++  ++N        V+L+ C    + Q WALYSDGTI
Sbjct: 461 WRVGDDVEPIVTKIVGFQEKCLEANYLENTNVSRYTKVFLDDCVLDRQQQRWALYSDGTI 520

Query: 219 RVDNYGSRDYCLTSQRNPSSVILVSNCDDKATQRWDINEDNFIYHPNTNLVLDVDRKPEG 278
           R D+  S        R+  S+I+++ C     QRW  N D  I +PN  LV+DV      
Sbjct: 521 RADSDRSLRVTADGHRSLDSIIILA-CKGWGNQRWVFNTDGTILNPNAKLVMDVKDSDVS 580

Query: 279 SQIVVIDTRDGTPTQQW 287
              +++    G P Q+W
Sbjct: 581 LLQIILHQSTGKPNQKW 595

[Close]
BLAST of MC10g0432.1 vs. ExPASy Swiss-Prot
Match: P11140 (Abrin-a OS=Abrus precatorius OX=3816 PE=1 SV=2)

HSP 1 Score: 117.1 bits (292), Expect = 3.3e-25
Identity = 74/254 (29.13%), Postives = 126/254 (49.61%), Query Frame = 0 

Query: 39  IVGRDGMCLDIVVPNVRPLYAQTQLSRC-DVAKQTQMWSILEDGTIRTKDNLYCLVPYND 98
           I GRDGMC+D V  N      +  + +C D  ++ Q+W++  D TIR+  N  CL  Y  
Sbjct: 279 IGGRDGMCVD-VYDNGYHNGNRIIMWKCKDRLEENQLWTLKSDKTIRS--NGKCLTTYGY 338

Query: 99  AYEAKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTAKPDNF---VILGTDAYTPSQ 158
           A  +  ++  C+  +     W+   +GT ++  S  VL+A+  +    + + T+ Y   Q
Sbjct: 339 APGSYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMRQ 398

Query: 159 SWEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNY 218
            W      SP V +I    + C+Q+  +    V++  CD   K Q WALY+DG+IR  + 
Sbjct: 399 GWRTGNNTSPFVTSISGYSDLCMQAQGSN---VWMADCDSNKKEQQWALYTDGSIR--SV 458

Query: 219 GSRDYCLTSQ--RNPSSVILVSNCDDKATQRWDINEDNFIYHPNTNLVLDVDRKPEGSQI 278
            + + CLTS+  +  S+++L+   +  A+QRW    D  IY    ++V+DV       + 
Sbjct: 459 QNTNNCLTSKDHKQGSTILLMGCSNGWASQRWVFKNDGSIYSLYDDMVMDVKGSDPSLKQ 518

Query: 279 VVIDTRDGTPTQQW 287
           +++    G P Q W
Sbjct: 519 IILWPYTGKPNQIW 524

[Close]
BLAST of MC10g0432.1 vs. NCBI nr
Match: XP_022149160.1 (nigrin b-like [Momordica charantia])

HSP 1 Score: 603 bits (1554), Expect = 8.77e-218
Identity = 288/288 (100.00%), Postives = 288/288 (100.00%), Query Frame = 0 

Query: 1   MRGMVVYIVVALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQ 60
           MRGMVVYIVVALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQ
Sbjct: 5   MRGMVVYIVVALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQ 64

Query: 61  TQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH 120
           TQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH
Sbjct: 65  TQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH 124

Query: 121 RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM 180
           RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM
Sbjct: 125 RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM 184

Query: 181 DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGSRDYCLTSQRNPSSVILVSNCDDKA 240
           DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGSRDYCLTSQRNPSSVILVSNCDDKA
Sbjct: 185 DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGSRDYCLTSQRNPSSVILVSNCDDKA 244

Query: 241 TQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTI 288
           TQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTI
Sbjct: 245 TQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTI 292

[Close]
BLAST of MC10g0432.1 vs. NCBI nr
Match: XP_022149159.1 (beta-galactoside-specific lectin 3-like [Momordica charantia])

HSP 1 Score: 503 bits (1296), Expect = 1.93e-178
Identity = 241/289 (83.39%), Postives = 258/289 (89.27%), Query Frame = 0 

Query: 1   MRGMVVYIVVALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQ 60
           M+ MVV IVVALSVLMS +  MAAYPYP YGSFGK SHIVGRDG+CLDI       +Y Q
Sbjct: 5   MKEMVVCIVVALSVLMSAHHAMAAYPYPRYGSFGKKSHIVGRDGLCLDIAKFEPAQVYIQ 64

Query: 61  TQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH 120
           TQLS C+ AKQTQ+W+ILEDGTIRTKD +YCLVPYNDAYEAKAVLGKC+EL+RS KKW H
Sbjct: 65  TQLSLCEAAKQTQLWTILEDGTIRTKDYVYCLVPYNDAYEAKAVLGKCAELMRSVKKWNH 124

Query: 121 RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM 180
           ++DGTFVHEN+G VLTAKPDNFV+LGTD  TPSQSWEATEGL+PSVVNI+W E  CLQSM
Sbjct: 125 KNDGTFVHENTGLVLTAKPDNFVVLGTDVNTPSQSWEATEGLTPSVVNIQWREGLCLQSM 184

Query: 181 DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGSR-DYCLTSQRNPSSVILVSNCDDK 240
           DNGAPFVYLN CDKKNKNQHWALYSDGTIRVDNYG R DYCLTSQRNPSSVILVSNCDDK
Sbjct: 185 DNGAPFVYLNSCDKKNKNQHWALYSDGTIRVDNYGKRSDYCLTSQRNPSSVILVSNCDDK 244

Query: 241 ATQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTI 288
           ATQRW INE+NFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTP QQWTI
Sbjct: 245 ATQRWGINEENFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPNQQWTI 293

[Close]
BLAST of MC10g0432.1 vs. NCBI nr
Match: XP_023551012.1 (nigrin b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 265 bits (676), Expect = 2.80e-84
Identity = 139/284 (48.94%), Postives = 181/284 (63.73%), Query Frame = 0 

Query: 10  VALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQTQLSRCDVA 69
           +AL+  ++ +  +AA    GYGSFG  SH+VGRDG+CL+ ++  +   Y  T LS C+  
Sbjct: 11  IALAFSLATHTAIAAAS--GYGSFGARSHLVGRDGLCLNALL--LFGYYTPTDLSLCEQR 70

Query: 70  KQTQMWSILEDGTIRTKDNLYCLVPYNDAYE---AKAVLGKCSELIRSDKKWKHRSDGTF 129
           K  Q+WSILEDGTIR  D  +CLVP + + E   A  V   CS  IR D KW    DGT 
Sbjct: 71  KSNQLWSILEDGTIRISDVRFCLVPKSQSTEFIGANVVAMDCSREIRHDMKWTKNKDGTI 130

Query: 130 VHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSMDNGAPF 189
            H+ SG VLTAKP   V +  +   PSQSWEATE  +P V NIKWL+N CL+S++    +
Sbjct: 131 HHDESGLVLTAKPGKTVTVEVNENLPSQSWEATETFNPMVANIKWLDNLCLKSIEGENSY 190

Query: 190 VYLNLCDKKNKNQHWALYSDGTIRVDNYGSRDYCLTS-QRNPSSVILVSNCDDKATQRWD 249
           V L  C + +KNQ WALY DGTIR + Y  R  CLTS +RN  ++++VS C DK  QRW 
Sbjct: 191 VTLKECSRDDKNQRWALYGDGTIRQNEY--RHLCLTSFERNYGAMVVVSRCADKPQQRWG 250

Query: 250 INEDNFIYHPNTNLVLDVDRK-PEGSQIVVIDTRDGTPTQQWTI 288
           + EDN I HPNTNLV+DV R+ P    ++V++  DG P+QQWTI
Sbjct: 251 LAEDNTINHPNTNLVMDVRREVPLLPPMIVVEKHDGAPSQQWTI 288

[Close]
BLAST of MC10g0432.1 vs. NCBI nr
Match: XP_022941584.1 (nigrin b-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 264 bits (674), Expect = 5.62e-84
Identity = 138/284 (48.59%), Postives = 183/284 (64.44%), Query Frame = 0 

Query: 10  VALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQTQLSRCDVA 69
           +AL+  ++ +  +AA    GYGSFG  +H+VGRDG+CLD ++  +   Y  T LS+C+  
Sbjct: 11  IALAFSLATHTAIAAAS--GYGSFGSRTHLVGRDGLCLDTML--LLGYYPPTHLSQCEQR 70

Query: 70  KQTQMWSILEDGTIRTKDNLYCLVPYNDAYE---AKAVLGKCSELIRSDKKWKHRSDGTF 129
           K +Q+WSILEDGTIR  D  +CLVP + + E   A  V   CS+ IR D KW    DGT 
Sbjct: 71  KSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDMKWTKNKDGTI 130

Query: 130 VHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSMDNGAPF 189
            H+ SG VLTAKP   V +  +   PSQSWEATE   P V NIKWL+N CL+S++    +
Sbjct: 131 HHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSIEGENSY 190

Query: 190 VYLNLCDKKNKNQHWALYSDGTIRVDNYGSRDYCLTS-QRNPSSVILVSNCDDKATQRWD 249
           V LN C + +KNQ WALY DGTIR + Y  R  CLTS +RN  ++++VS C DK  QRW 
Sbjct: 191 VTLNECSRDDKNQRWALYGDGTIRQNEY--RHLCLTSSERNYGAMVVVSRCADKPQQRWG 250

Query: 250 INEDNFIYHPNTNLVLDVDRK-PEGSQIVVIDTRDGTPTQQWTI 288
           + ED+ I HPNTNLV+DV R+ P    ++V+D  DG  +Q+WTI
Sbjct: 251 LAEDSTINHPNTNLVMDVRREVPLLPPMIVVDKHDGAASQRWTI 288

[Close]
BLAST of MC10g0432.1 vs. NCBI nr
Match: XP_022982216.1 (ribosome-inactivating protein SNAI-like [Cucurbita maxima])

HSP 1 Score: 263 bits (672), Expect = 9.92e-84
Identity = 132/264 (50.00%), Postives = 172/264 (65.15%), Query Frame = 0 

Query: 30  YGSFGKISHIVGRDGMCLDIVVPNVRPLYAQTQLSRCDVAKQTQMWSILEDGTIRTKDNL 89
           YG+FG  +H+VGRDG+CLD ++  +   Y QT LS+C   K +Q+W+ILEDGTIR  D  
Sbjct: 25  YGAFGGRTHLVGRDGLCLDTML--LSGYYPQTDLSQCQPRKLSQLWTILEDGTIRASDVR 84

Query: 90  YCLVP---YNDAYEAKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTAKPDNFVILG 149
           YCLVP   +        V+  C++ +R D KW  + DGT  H+ SG VLTAKP   V L 
Sbjct: 85  YCLVPKTQFIGPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLE 144

Query: 150 TDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSD 209
            +   PSQSWEATE L P V NIKWL+N CL+S +    +V LN C + +KNQ WALY D
Sbjct: 145 ANEDAPSQSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGD 204

Query: 210 GTIRVDNYGSRDYCLTS-QRNPSSVILVSNCDDKATQRWDINEDNFIYHPNTNLVLDVDR 269
           GTIR + Y  R  CLTS +RN  ++++VS C DK  QRW + EDN I HPNTNLV+DV R
Sbjct: 205 GTIRQNEY--RHLCLTSSERNYGALVVVSRCADKPQQRWGLAEDNTINHPNTNLVMDVHR 264

Query: 270 K-PEGSQIVVIDTRDGTPTQQWTI 288
           + P    ++V+D  DG P+Q+W+I
Sbjct: 265 EVPLLPPMIVVDKHDGAPSQRWSI 284

[Close]
BLAST of MC10g0432.1 vs. ExPASy TrEMBL
Match: A0A6J1D4Y7 (nigrin b-like OS=Momordica charantia OX=3673 GN=LOC111017650 PE=3 SV=1)

HSP 1 Score: 603 bits (1554), Expect = 4.25e-218
Identity = 288/288 (100.00%), Postives = 288/288 (100.00%), Query Frame = 0 

Query: 1   MRGMVVYIVVALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQ 60
           MRGMVVYIVVALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQ
Sbjct: 5   MRGMVVYIVVALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQ 64

Query: 61  TQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH 120
           TQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH
Sbjct: 65  TQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH 124

Query: 121 RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM 180
           RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM
Sbjct: 125 RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM 184

Query: 181 DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGSRDYCLTSQRNPSSVILVSNCDDKA 240
           DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGSRDYCLTSQRNPSSVILVSNCDDKA
Sbjct: 185 DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGSRDYCLTSQRNPSSVILVSNCDDKA 244

Query: 241 TQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTI 288
           TQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTI
Sbjct: 245 TQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTI 292

[Close]
BLAST of MC10g0432.1 vs. ExPASy TrEMBL
Match: A0A6J1D675 (beta-galactoside-specific lectin 3-like OS=Momordica charantia OX=3673 GN=LOC111017649 PE=3 SV=1)

HSP 1 Score: 503 bits (1296), Expect = 9.32e-179
Identity = 241/289 (83.39%), Postives = 258/289 (89.27%), Query Frame = 0 

Query: 1   MRGMVVYIVVALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQ 60
           M+ MVV IVVALSVLMS +  MAAYPYP YGSFGK SHIVGRDG+CLDI       +Y Q
Sbjct: 5   MKEMVVCIVVALSVLMSAHHAMAAYPYPRYGSFGKKSHIVGRDGLCLDIAKFEPAQVYIQ 64

Query: 61  TQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH 120
           TQLS C+ AKQTQ+W+ILEDGTIRTKD +YCLVPYNDAYEAKAVLGKC+EL+RS KKW H
Sbjct: 65  TQLSLCEAAKQTQLWTILEDGTIRTKDYVYCLVPYNDAYEAKAVLGKCAELMRSVKKWNH 124

Query: 121 RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM 180
           ++DGTFVHEN+G VLTAKPDNFV+LGTD  TPSQSWEATEGL+PSVVNI+W E  CLQSM
Sbjct: 125 KNDGTFVHENTGLVLTAKPDNFVVLGTDVNTPSQSWEATEGLTPSVVNIQWREGLCLQSM 184

Query: 181 DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGSR-DYCLTSQRNPSSVILVSNCDDK 240
           DNGAPFVYLN CDKKNKNQHWALYSDGTIRVDNYG R DYCLTSQRNPSSVILVSNCDDK
Sbjct: 185 DNGAPFVYLNSCDKKNKNQHWALYSDGTIRVDNYGKRSDYCLTSQRNPSSVILVSNCDDK 244

Query: 241 ATQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTI 288
           ATQRW INE+NFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTP QQWTI
Sbjct: 245 ATQRWGINEENFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPNQQWTI 293

[Close]
BLAST of MC10g0432.1 vs. ExPASy TrEMBL
Match: A0A6J1FNV0 (nigrin b-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=3 SV=1)

HSP 1 Score: 264 bits (674), Expect = 2.72e-84
Identity = 138/284 (48.59%), Postives = 183/284 (64.44%), Query Frame = 0 

Query: 10  VALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQTQLSRCDVA 69
           +AL+  ++ +  +AA    GYGSFG  +H+VGRDG+CLD ++  +   Y  T LS+C+  
Sbjct: 11  IALAFSLATHTAIAAAS--GYGSFGSRTHLVGRDGLCLDTML--LLGYYPPTHLSQCEQR 70

Query: 70  KQTQMWSILEDGTIRTKDNLYCLVPYNDAYE---AKAVLGKCSELIRSDKKWKHRSDGTF 129
           K +Q+WSILEDGTIR  D  +CLVP + + E   A  V   CS+ IR D KW    DGT 
Sbjct: 71  KSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDMKWTKNKDGTI 130

Query: 130 VHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSMDNGAPF 189
            H+ SG VLTAKP   V +  +   PSQSWEATE   P V NIKWL+N CL+S++    +
Sbjct: 131 HHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSIEGENSY 190

Query: 190 VYLNLCDKKNKNQHWALYSDGTIRVDNYGSRDYCLTS-QRNPSSVILVSNCDDKATQRWD 249
           V LN C + +KNQ WALY DGTIR + Y  R  CLTS +RN  ++++VS C DK  QRW 
Sbjct: 191 VTLNECSRDDKNQRWALYGDGTIRQNEY--RHLCLTSSERNYGAMVVVSRCADKPQQRWG 250

Query: 250 INEDNFIYHPNTNLVLDVDRK-PEGSQIVVIDTRDGTPTQQWTI 288
           + ED+ I HPNTNLV+DV R+ P    ++V+D  DG  +Q+WTI
Sbjct: 251 LAEDSTINHPNTNLVMDVRREVPLLPPMIVVDKHDGAASQRWTI 288

[Close]
BLAST of MC10g0432.1 vs. ExPASy TrEMBL
Match: A0A6J1IYQ6 (ribosome-inactivating protein SNAI-like OS=Cucurbita maxima OX=3661 GN=LOC111481109 PE=4 SV=1)

HSP 1 Score: 263 bits (672), Expect = 4.80e-84
Identity = 132/264 (50.00%), Postives = 172/264 (65.15%), Query Frame = 0 

Query: 30  YGSFGKISHIVGRDGMCLDIVVPNVRPLYAQTQLSRCDVAKQTQMWSILEDGTIRTKDNL 89
           YG+FG  +H+VGRDG+CLD ++  +   Y QT LS+C   K +Q+W+ILEDGTIR  D  
Sbjct: 25  YGAFGGRTHLVGRDGLCLDTML--LSGYYPQTDLSQCQPRKLSQLWTILEDGTIRASDVR 84

Query: 90  YCLVP---YNDAYEAKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTAKPDNFVILG 149
           YCLVP   +        V+  C++ +R D KW  + DGT  H+ SG VLTAKP   V L 
Sbjct: 85  YCLVPKTQFIGPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLE 144

Query: 150 TDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSD 209
            +   PSQSWEATE L P V NIKWL+N CL+S +    +V LN C + +KNQ WALY D
Sbjct: 145 ANEDAPSQSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGD 204

Query: 210 GTIRVDNYGSRDYCLTS-QRNPSSVILVSNCDDKATQRWDINEDNFIYHPNTNLVLDVDR 269
           GTIR + Y  R  CLTS +RN  ++++VS C DK  QRW + EDN I HPNTNLV+DV R
Sbjct: 205 GTIRQNEY--RHLCLTSSERNYGALVVVSRCADKPQQRWGLAEDNTINHPNTNLVMDVHR 264

Query: 270 K-PEGSQIVVIDTRDGTPTQQWTI 288
           + P    ++V+D  DG P+Q+W+I
Sbjct: 265 EVPLLPPMIVVDKHDGAPSQRWSI 284

[Close]
BLAST of MC10g0432.1 vs. ExPASy TrEMBL
Match: A0A6J1FU34 (nigrin b-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=4 SV=1)

HSP 1 Score: 261 bits (667), Expect = 2.92e-83
Identity = 133/265 (50.19%), Postives = 173/265 (65.28%), Query Frame = 0 

Query: 29  GYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQTQLSRCDVAKQTQMWSILEDGTIRTKDN 88
           GYG+FG  +H+VGRDG+CLD ++  +   Y  T LS+C+  K +Q+WSILEDGTIR  D 
Sbjct: 26  GYGAFGGRTHLVGRDGLCLDTML--LLGYYPPTHLSQCEQRKSSQLWSILEDGTIRISDV 85

Query: 89  LYCLVPYNDAYE---AKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTAKPDNFVIL 148
            +CLVP + + E   A  V   CS+ IR D KW    DGT  H+ SG VLTAKP   V +
Sbjct: 86  RFCLVPKSQSTEFVGANVVAMDCSKEIRHDMKWTKNKDGTIHHDESGLVLTAKPGRTVTV 145

Query: 149 GTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYS 208
             +   PSQSWEATE   P V NIKWL+N CL+S++    +V LN C + +KNQ WALY 
Sbjct: 146 EVNENLPSQSWEATETFIPMVANIKWLDNLCLKSIEGENSYVTLNECSRDDKNQRWALYG 205

Query: 209 DGTIRVDNYGSRDYCLTS-QRNPSSVILVSNCDDKATQRWDINEDNFIYHPNTNLVLDVD 268
           DGTIR + Y  R  CLTS +RN  ++++VS C DK  QRW + ED+ I HPNTNLV+DV 
Sbjct: 206 DGTIRQNEY--RHLCLTSSERNYGAMVVVSRCADKPQQRWGLAEDSTINHPNTNLVMDVR 265

Query: 269 RK-PEGSQIVVIDTRDGTPTQQWTI 288
           R+ P    ++V+D  DG  +Q+WTI
Sbjct: 266 REVPLLPPMIVVDKHDGAASQRWTI 286

The following BLAST results are available for this feature:
BLAST of MC10g0432.1 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P826831.8e-2631.52Beta-galactoside-specific lectin 3 OS=Viscum album OX=3972 PE=1 SV=2[more]
P285901.5e-2528.46Abrin-c OS=Abrus precatorius OX=3816 PE=1 SV=1[more]
Q060772.5e-2528.06Abrin-b OS=Abrus precatorius OX=3816 PE=1 SV=1[more]
Q9M6542.5e-2529.57Ribosome-inactivating protein PMRIPm OS=Polygonatum multiflorum OX=45371 GN=RIPm... [more]
P111403.3e-2529.13Abrin-a OS=Abrus precatorius OX=3816 PE=1 SV=2[more]
BLAST of MC10g0432.1 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022149160.18.77e-218100.00nigrin b-like [Momordica charantia][more]
XP_022149159.11.93e-17883.39beta-galactoside-specific lectin 3-like [Momordica charantia][more]
XP_023551012.12.80e-8448.94nigrin b-like [Cucurbita pepo subsp. pepo][more]
XP_022941584.15.62e-8448.59nigrin b-like isoform X1 [Cucurbita moschata][more]
XP_022982216.19.92e-8450.00ribosome-inactivating protein SNAI-like [Cucurbita maxima][more]
BLAST of MC10g0432.1 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1D4Y74.25e-218100.00nigrin b-like OS=Momordica charantia OX=3673 GN=LOC111017650 PE=3 SV=1[more]
A0A6J1D6759.32e-17983.39beta-galactoside-specific lectin 3-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1FNV02.72e-8448.59nigrin b-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=3 SV=1[more]
A0A6J1IYQ64.80e-8450.00ribosome-inactivating protein SNAI-like OS=Cucurbita maxima OX=3661 GN=LOC111481... [more]
A0A6J1FU342.92e-8350.19nigrin b-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=4 SV=1[more]
BLAST of MC10g0432.1 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000772Ricin B, lectin domainSMARTSM00458ricin_3coord: 163..288
e-value: 2.4E-17
score: 73.7
coord: 33..159
e-value: 3.2E-9
score: 46.6
IPR000772Ricin B, lectin domainPFAMPF00652Ricin_B_lectincoord: 38..156
e-value: 9.8E-15
score: 54.9
coord: 173..286
e-value: 1.3E-20
score: 73.9
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 163..288
score: 28.802999
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 32..159
score: 21.4335
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 167..288
e-value: 3.33711E-22
score: 87.561
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 38..157
e-value: 4.77937E-14
score: 65.6046
NoneNo IPR availableGENE3D2.80.10.50coord: 164..288
e-value: 1.3E-32
score: 114.1
NoneNo IPR availableGENE3D2.80.10.50coord: 35..159
e-value: 4.5E-23
score: 83.5
NoneNo IPR availablePANTHERPTHR33453:SF23RRNA N-GLYCOSIDASEcoord: 38..288
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 38..288
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 167..288
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 34..157

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MC10g0432MC10g0432gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MC10g0432.1-cdsMC10g0432.1-cds-MC10:3480898..3481761CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MC10g0432.1MC10g0432.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017148 negative regulation of translation
molecular_function GO:0030598 rRNA N-glycosylase activity
molecular_function GO:0030246 carbohydrate binding