MC09g1598.1 (mRNA) Bitter gourd (Dali-11) v1

BLAST of MC09g1598.1 vs. ExPASy Swiss-Prot
Match: Q59536 (Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.9e-94
Identity = 230/753 (30.54%), Postives = 369/753 (49.00%), Query Frame = 0

Query: 48  PAAKKVPFKYSVHGVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSE 107
           P AK++P  + +HG   +D ++W+ + D+ ++  YL  EN Y    M   Q    +++  
Sbjct: 4   PIAKRIPHPHELHGDVREDDYYWLKDRDNTEVIQYLEEENRYYHEIMRPLQEQTEQIYES 63

Query: 108 MTSRMPAKVSTPPEPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKE 167
           M  R+P      P   G +FYY  +   K+YP+  R     K    + L+Q A       
Sbjct: 64  MVDRVPDSEMKVPVQHGQFFYYSRLDKNKQYPIYAR-----KQAASRALLQDA------- 123

Query: 168 EEEVLLDWNEIAKHYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKS 227
            EEV+LD NE+A+   Y+ V   R++ DH+ LAY  +  G++ + + +KDL +G ++   
Sbjct: 124 TEEVVLDLNELAEEDDYLSVTVQRMTTDHSRLAYLENRDGTDRYTIYIKDLNTGELLSDR 183

Query: 228 QKGVV---SLAWAEEGRTLFYTQSDENQRP---YRQNSVKDVS----VFVENDPNFCVDV 287
              V    S+ W   G  +FYT  DE+QRP   +R     DV     +F E D  F + +
Sbjct: 184 VPNVYIYGSMEWCRCGDYIFYTTVDEHQRPCQLWRHRLGSDVESDELIFEEKDDTFTLFI 243

Query: 288 TSTKDGKFITVY----------IIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNA 347
           + ++ GKFI VY          +ID ++PLS LQ + +R  GI Y +EH      ILTN 
Sbjct: 244 SKSQSGKFIFVYSSSKTTSEIHMIDTDSPLSPLQLVDERRDGILYDVEHWEDDLLILTNE 303

Query: 348 PLEKNGDCSKEEYYVARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVS 407
                       + + RC + D+ S    + +  +E+  +Q+M  F   L++   + G++
Sbjct: 304 --------GALNFQLLRCPLNDLSSK--VNVVEYNEERYLQEMYPFRDKLLIAGRENGLT 363

Query: 408 MLCAINLPLDTNHKHRLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPD 467
            +  +         H  E++++  W  P                   LY V + S     
Sbjct: 364 QIWVV---------HDGELQQIS-WDEP-------------------LYTVAVLSE---- 423

Query: 468 LVVDYDMSKRVFSIIQQEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISK 527
               YD ++    +IQ E +     +  KT     L   E    +    + +   SQ  +
Sbjct: 424 --QSYDTNE---VLIQYESL-----LTPKTTFGLNLQTGEKQCLQVAPVSGEYDRSQFRQ 483

Query: 528 DFSDAYCCERKEVISHDGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRL 587
                   E+       G+++P+T +Y       G +P +L+GYG+YG   D  + PYRL
Sbjct: 484 --------EQLWATGRSGVKVPMTAVYLEGALDNGPAPLILYGYGSYGSNSDPRFDPYRL 543

Query: 588 SLLDRGFVLAFADVRGGGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLG 647
            LL++G V   A VRGG   G   W+  G    K+N+  DFI+ AK L+D +Y    ++ 
Sbjct: 544 PLLEKGIVFVTAQVRGGSEMG-RGWYEDGKMQNKRNTFTDFIAAAKHLIDQNYTSPTKMA 603

Query: 648 SIGYSAGGLLVGAAINMRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPK 707
           + G SAGGLLVGA  NM  +LF+  +  VPF+D+  T+LD S+PLT L+++E+G+P+  +
Sbjct: 604 ARGGSAGGLLVGAVANMAGELFKVIVPAVPFVDVVTTMLDTSIPLTTLEWDEWGDPRKQE 663

Query: 708 QFESILNYSPYDNISRGSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAIL 767
            +  + +YSPYDN+     YP M +T+   D RVG +E AKWVA++R       + + ++
Sbjct: 664 DYFYMKSYSPYDNV-EAKDYPHMYITTGINDPRVGYFEPAKWVARLRAVKTD--NNTLVM 679

Query: 768 KTNMLGGHFGEGGLYGGCEETAYEYAFLIKVLG 781
           KTNM  GHFG+ G +   +E A  YAF++  LG
Sbjct: 724 KTNMGAGHFGKSGRFNHLKEAAESYAFILDKLG 679

BLAST of MC09g1598.1 vs. ExPASy Swiss-Prot
Match: O07834 (Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 2.2e-79
Identity = 210/785 (26.75%), Postives = 362/785 (46.11%), Query Frame = 0

Query: 21  PSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVHGVTLQDPFHWM--SNTDDPD 80
           P+S   ++++  S     + +  +  P  AKK     + HG    D ++W+     ++ +
Sbjct: 3   PTSLLLAATVLMSTPITSALAASATPPDVAKKPHVVKAPHGAERNDEYYWLRDDKRENKE 62

Query: 81  LADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPPEPWGPWFYYQYIPAGKEY 140
           +  YL  EN Y +A MA  + L+ +L+ E+ +R+    ++ P     W+YY     GK+Y
Sbjct: 63  MLAYLNAENAYTDAVMAPLKPLEDKLYDEVVARIKQDDASVPYRERGWWYYARFVTGKDY 122

Query: 141 PVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAKHYGYVHVGTCRVSPDHNF 200
           PV  RR     +  +      A G+F    E+VLLD N +     Y +VG   VS D+  
Sbjct: 123 PVHARRKDGPGVDAVSIQAANAAGDFA--GEQVLLDVNALGAGKDYYNVGDYEVSQDNRL 182

Query: 201 LAYTVDITGSEHFMLQVKDLGSGLIIPKS-QKGVVSLAWAEEGRTLFYTQSDENQRPYRQ 260
           LAY  D  G   + ++ K+L +G ++P +      +L W+++GRTLFY   D      ++
Sbjct: 183 LAYADDTNGRRQYTIRFKNLDTGELLPDTVTNAEPNLVWSDDGRTLFYVDKDPETLLSKR 242

Query: 261 --------NSVKDVSVFVENDPNFCVDVTSTKDGKFITVYIIDANNPLSGLQRIHKRIPG 320
                    + +D  V+ E D +F + +  ++D KFI + +   +   S ++      PG
Sbjct: 243 VKAHVLGTPASQDALVYEEEDDSFYMGIGRSRDDKFICISV--ESTVSSEMRCTPAASPG 302

Query: 321 -----------IQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYVARCRVEDIKSSDWQDA 380
                      ++Y  +H    + I TNA    N       + +     +     DW+D 
Sbjct: 303 VFTVLAPRERDVEYQADHLGDRWVIRTNADGATN-------FKIVTAPTDSTSRKDWKDW 362

Query: 381 ILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKHRLEIEKLDPWFFPLPS 440
           +   +D  ++  ++F G  V+      +  L  I     +++       K D   +   S
Sbjct: 363 VAHRDDVFVEGFELFDGFSVVAERANALESLRVIKADGSSDY------VKADESAY---S 422

Query: 441 NSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSIIQQEEVQVKHDVQLKTC 500
              S  P +  D++   Y   +++P                           +++  KT 
Sbjct: 423 MGLSANPETGTDWLRYSY-TSMTTPA------------------------TTYEINTKTG 482

Query: 501 LPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVISHDG-IRIPLTILYSPV 560
              +L  + V   +                 +  Y  ER    + DG  +IP+T++Y   
Sbjct: 483 ERRQLKQQPVPGYD-----------------ASKYVTERVWAPARDGKTKIPVTLVYRKD 542

Query: 561 NFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRSGS 620
               G++P + + YG+YG  +D ++    +SLLDRG V A A +RGG   G  +W+  G 
Sbjct: 543 VARDGKAPMLQYAYGSYGASMDPNFSITNVSLLDRGVVYALAHIRGGQEMG-RAWYDDGK 602

Query: 621 GLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAINMRPDLFRAAILKVP 680
              K N+  DFI    +LV   Y  K+++ ++G SAGGLL+GA  NM P+ ++  +  VP
Sbjct: 603 LYNKINTFTDFIDVTDYLVKEGYAAKDRVAAMGGSAGGLLMGAVSNMAPEKYKVILTLVP 662

Query: 681 FLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNISRGSCYPPMLVTSSFR 740
           F+D+  T+LDP++PLT  +Y+E+GNP+    ++ IL YSPYDN+ +   YP M V +   
Sbjct: 663 FVDVVTTMLDPTIPLTTNEYDEWGNPEEKGYYDYILTYSPYDNL-QAKAYPAMFVGTGLW 721

Query: 741 DARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIK 783
           D++V  WE AK+VA++RD    +     + +TNM  GH G+ G +    E A  +AF++ 
Sbjct: 723 DSQVQYWEPAKYVARLRDLNTGK--GPVVFRTNMEAGHGGKSGRFRQYRERAEMFAFMLD 721

BLAST of MC09g1598.1 vs. ExPASy Swiss-Prot
Match: P24555 (Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2)

HSP 1 Score: 298.5 bits (763), Expect = 2.2e-79
Identity = 206/750 (27.47%), Postives = 351/750 (46.80%), Query Frame = 0

Query: 48  PAAKKVPFKYSVHGVTLQDPFHWM--SNTDDPDLADYLRRENLYAEAFMADTQILQRRLF 107
           P A ++P   ++HG T  D ++W+       P++ DYL++EN Y    MA  Q LQ R+ 
Sbjct: 3   PKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDRIL 62

Query: 108 SEMTSRMPAKVSTPPEPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFG 167
            E+  R+P +  + P     + Y      G EY +  R+    +  W             
Sbjct: 63  KEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQSAFSE-EW------------- 122

Query: 168 KEEEEVLLDWNEIAKHYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIP 227
            +E E LLD N+ A H  +  +G   ++PD+  +A   D      + ++ ++L +G   P
Sbjct: 123 -DEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNLETGNWYP 182

Query: 228 KSQKGV-VSLAWAEEGRTLFYTQSDE-NQRPYR-------QNSVKDVSVFVENDPNFCVD 287
           +    V  S  WA +    +Y +       PY+         + +D  ++ E D  + V 
Sbjct: 183 ELLDNVEPSFVWANDSWIFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVS 242

Query: 288 VTSTKDGKFITVYIIDANNPLSGLQRIH----------KRIPGIQYFLEHHFGFFYILTN 347
           +  T    ++ +++  A      L               R    +Y L+H+   FY+ +N
Sbjct: 243 LHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSN 302

Query: 348 APLEKNGDCSKEEYYVARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGV 407
               ++G    + + + R R+ D     W++ I   E+  ++   +F+  LV+   + G+
Sbjct: 303 ----RHG----KNFGLYRTRMRD--EQQWEELIPPRENIMLEGFTLFTDWLVVEERQRGL 362

Query: 408 SMLCAINLPLDTNHKHRLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMP 467
           + L  IN       +  + I   DP +    +         N +  ++  R   SS   P
Sbjct: 363 TSLRQIN----RKTREVIGIAFDDPAYVTWIA--------YNPEPETARLRYGYSSMTTP 422

Query: 468 DLVVDYDMSKRVFSIIQQEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQIS 527
           D + + DM                                   T E ++      ++++ 
Sbjct: 423 DTLFELDM----------------------------------DTGERRVL----KQTEVP 482

Query: 528 KDFSDAYCCERKEVISHDGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYR 587
             ++  Y  E   +++ DG+ +P++++Y   +F KG +P +++GYG+YG  +D  +   R
Sbjct: 483 GFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSR 542

Query: 588 LSLLDRGFVLAFADVRGGGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQL 647
           LSLLDRGFV A   VRGGG  G   W+  G  L+K+N+ +D++     L+   Y   +  
Sbjct: 543 LSLLDRGFVYAIVHVRGGGELG-QQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLC 602

Query: 648 GSIGYSAGGLLVGAAINMRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLP 707
            ++G SAGG+L+G AIN RP+LF   I +VPF+D+  T+LD S+PLT  ++EE+GNPQ P
Sbjct: 603 YAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDP 662

Query: 708 KQFESILNYSPYDNISRGSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAI 767
           + +E + +YSPYDN++    YP +LVT+   D++V  WE AKWVAK+R+          +
Sbjct: 663 QYYEYMKSYSPYDNVT-AQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTD--DHLLL 673

Query: 768 LKTNMLGGHFGEGGLYGGCEETAYEYAFLI 777
           L T+M  GH G+ G +   E  A EYAFL+
Sbjct: 723 LCTDMDSGHGGKSGRFKSYEGVAMEYAFLV 673

BLAST of MC09g1598.1 vs. ExPASy Swiss-Prot
Match: P55656 (Uncharacterized peptidase y4sO OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a01580 PE=3 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 2.4e-57
Identity = 188/779 (24.13%), Postives = 335/779 (43.00%), Query Frame = 0

Query: 44  SESPPAAKKVPFKYSVHGVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRR 103
           S  PP  +       +H     D + W+ + +DPD+  YL  EN YA+   +    L+  
Sbjct: 5   SLQPPLPRSERRIRVLHNDVTIDSYGWLRDREDPDVLAYLEAENHYADEVTSYVAELKAD 64

Query: 104 LFSEMTSRMPAKVSTPPEPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGN 163
           L +E+  R     + PP   G +FY+Q   +G  +             W ++ V      
Sbjct: 65  LIAEIEKRDSCDGAPPPFQVGFFFYFQKSQSGLLHS-----------AWWRRPVTGG--- 124

Query: 164 FGKEEEEVLLDWNEIAKHYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLI 223
                EE++ D N +     +  +G    S D  ++A++ D+ G+E + L+V+D+ +G  
Sbjct: 125 ----PEELVFDPNTLPGAEVFYSLGALEPSDDGRYIAFSFDLIGNERYELRVRDMTNGRE 184

Query: 224 IPKSQKGVVSLAWAEEGRTLFYTQSDENQRPYRQNSVKDVS------VFVENDPNFCVDV 283
           I +       L WA + RTLF+T+   ++R + +    DV       VF E +    + V
Sbjct: 185 IWRDPSRAGRLVWAADNRTLFFTRERADRRQHDRVVRLDVETGRSEVVFEEVNERLALVV 244

Query: 284 TSTKDGKFI-------------------TVYIIDANNPLSGLQRIHKRIPGIQYFLEHHF 343
             +  G ++                    V+ + A  P    +RI  R  G + + EH  
Sbjct: 245 RRSGSGAYLFIDVIITSDMSSRIQRAAAEVWCLPAERPTDMWRRILARELGHEIYAEHWG 304

Query: 344 GFFYILTNAPLEKNGDCSKEEYYVARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLV 403
             F    N         +     + R  ++D   S WQ+ +      +++++ +   H++
Sbjct: 305 NEFLFRVND--------TGPNLRLVRTAIDDTSPSRWQEVVPHRAGITLEEIHVLEEHVI 364

Query: 404 LFVNKMGVSMLCAINLPLDTNHKHRLEIEKLDPWFFPLPSNSCSVAPG---------SNH 463
           +   +        I   L  +H++     ++ P   P+  +SC+V  G         + H
Sbjct: 365 VLERE-------GIQPRLVAHHRN----GRVGPSIVPV-EHSCTVTVGLSAGGSYSCARH 424

Query: 464 DFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSIIQQEEVQVKHDVQLKTCLPDELDVEEVS 523
            +  S     + S V PD+ + +D+                                   
Sbjct: 425 PYRVSALTYKICSFVTPDIFIQHDL----------------------------------L 484

Query: 524 TAENKIANFQNSESQISKDFSDAYCCERKEVISHDGIRIPLTILYSPVNFHKGRSPGVLH 583
           T ++K+  ++   S    +  +A     K   + DG+ +P++I+        G  P +L+
Sbjct: 485 TDKSKVL-YRTLVSGFEPELYEARVVMAK---AEDGVEVPISIVARRDRGEDG--PVLLN 544

Query: 584 GYGAYGEILDKSWCPY------RLSLLDRGFVLAFADVRGGGGGGDSSWHRSGSGLEKQN 643
            YG YG     ++  +      RLSLLDRG       VRGGG  G  +WH + +  +K+ 
Sbjct: 545 VYGCYGAQSLPAFFGWPSSMTARLSLLDRGVAFGIVHVRGGGELG-RAWHEAATRDQKRL 604

Query: 644 SIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAINMRPDLFRAAILKVPFLDICN 703
           +  D I+ A+ LV++ +  ++ +   G SAGG  V AA  +RPDLFRA + +VP  DI +
Sbjct: 605 THTDLIAAAECLVEHRFASRDGIVIEGRSAGGGTVLAAAVLRPDLFRAVLAEVPLADIID 664

Query: 704 TLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNISRGSCYPPMLVTSSFRDARVGV 763
           T LD +LP  + +  E+G+P L   ++ + +Y PY N++    YPP  + ++  D++V  
Sbjct: 665 TELDFTLPYALRETAEYGDPHLANDYQYLRSYDPYYNLTPDRRYPPTYIDAALHDSQVLY 703

Query: 764 WEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVLGTS 783
           ++ A++VA+ R     R     I +T M+GGH G     G  EE A+  A+++  LG S
Sbjct: 725 YQPARYVAQRRSKAVDR-DPDLIFRTRMIGGHMGVSHGPGVAEEAAFRMAWILHRLGQS 703

BLAST of MC09g1598.1 vs. ExPASy Swiss-Prot
Match: P55627 (Uncharacterized peptidase y4qF OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a01920 PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 4.5e-56
Identity = 188/773 (24.32%), Postives = 332/773 (42.95%), Query Frame = 0

Query: 47  PPAAKKVPFKYSVHGVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFS 106
           PP  +  P    +H     D + W+ + ++PD+  YL  EN YAE   A  + L+  L +
Sbjct: 39  PPLPRAEPRIRVLHDDVTVDRYGWLRDRENPDVRAYLEAENSYAEQATAHLRRLKTELIA 98

Query: 107 EMTSRMPAKVSTPPEPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGK 166
           E+  R P + +TPP   GP+ Y+Q    G  +PV           W ++ V         
Sbjct: 99  EIEGRQPCEGATPPFQVGPFDYFQGHERGLPHPV-----------WWRRPVTGG------ 158

Query: 167 EEEEVLLDWNEIAKHYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPK 226
              E++LD N I     +  +G    S D  +LA++VD+ G+E + L+V+D+  G  + +
Sbjct: 159 -SAELVLDPNAIPGADVFYWLGVFEPSDDGRYLAFSVDLIGAERYELRVRDMSDGRDVWR 218

Query: 227 SQKGVVSLAWAEEGRTLFYTQSDENQRPYRQNSVKDVS------VFVENDPNFCVDVTST 286
               V  + WA +  TLF+T+   ++R + Q    +V       VF E +    V V  +
Sbjct: 219 DAGSVGQVVWAADNHTLFFTRERPDRRQHHQIVRLNVGRGNSEVVFEEANERLAVLVRRS 278

Query: 287 KDGKFI-------------------TVYIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFF 346
           + G ++                    V+ + A+ P    +RI  R  G Q + EH +  F
Sbjct: 279 QSGAWLFLDVLTTSDMSSYVQRGAAEVWCLPADEPGGQWRRIVMRELGHQIYAEHWYDRF 338

Query: 347 YILTNAPLEKNGDCSKEEYYVARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVL-- 406
                       D +   + +    ++D   S W++ +      +I ++ +   HLVL  
Sbjct: 339 LFRV--------DDAGPYWRLVSAPIDDPSPSRWEEVVPHRAGVTIDEIHVLEQHLVLLE 398

Query: 407 -------FVNKMGVSMLCAINLPLDTNHKHRLEIEKLDPWFFPLPSNSCSVAPGSNHDFM 466
                   +++     + A+ +P + +   R+           L +  C  A  + H F 
Sbjct: 399 REGLRPRLISRNRSGRVGAVIVPDEPSCTIRV----------GLSAGGCYSA--ARHPFR 458

Query: 467 SSLYRVVLSSPVMPDLVVDYDMSKRVFSIIQQEEVQVKHDVQLKTCLPDELDVEEVSTAE 526
           SS     +SS V PD  +++D +               +D  +  C             E
Sbjct: 459 SSKLTYSVSSFVTPDTFIEHDFA---------------NDRSVVLC-------------E 518

Query: 527 NKIANFQNSESQISKDFSDAYCCERKEVISHDGIRIPLTILYSPVNFHKGRSPGVLHGYG 586
            ++  +  ++   +   ++A           DG+++P++++        G  P +L  YG
Sbjct: 519 ARVPGYDATQYLATVVMAEA----------EDGVQVPISLVARRDRTSPG--PVLLSVYG 578

Query: 587 AYGEILDKSWCPY------RLSLLDRGFVLAFADVRGGGGGGDSSWHRSGSGLEKQNSIH 646
            YG     S+  +      RLSLLDR        VRGGG  G   WH + +  +K+ +  
Sbjct: 579 CYGIPRLPSFLAWPSSMTARLSLLDREVAFGIVHVRGGGELG-RPWHDAATRDQKRITHT 638

Query: 647 DFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAINMRPDLFRAAILKVPFLDICNTLL 706
           D IS  + L++  +  ++ +   G S GG  V A    RP+LFRA + +VP  DI +T L
Sbjct: 639 DLISATEGLIERGFATRDGVVIEGKSGGGGTVLATAVFRPNLFRAVVAEVPLADIIDTQL 698

Query: 707 DPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNISRGSCYPPMLVTSSFRDARVGVWEA 766
           D ++P T+ +  E+G+PQ   ++  + +Y PY N+S     PP  V ++  D +V  ++ 
Sbjct: 699 DSTMPYTLKETAEYGDPQDAYEYRYLRSYDPYYNLSPERSLPPTYVDAALDDGQVIYYQP 731

Query: 767 AKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVL 780
           A++VA+ R     R     + +  M+GGH G     G  E+ A+  A+++  L
Sbjct: 759 ARYVAQRRSCATDR-DPDLVFRIRMVGGHSGPSHGPGIAEQAAFRMAWVLDQL 731

BLAST of MC09g1598.1 vs. NCBI nr
Match: XP_022149039.1 (uncharacterized protein LOC111017556 [Momordica charantia])

HSP 1 Score: 1586 bits (4107), Expect = 0.0
Identity = 780/802 (97.26%), Postives = 782/802 (97.51%), Query Frame = 0

Query: 1   MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH 60
           MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH
Sbjct: 17  MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH 76

Query: 61  GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP 120
           GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP
Sbjct: 77  GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP 136

Query: 121 EPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAK 180
           EPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAK
Sbjct: 137 EPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAK 196

Query: 181 HYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQKGVVSLAWAEEG 240
           HYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQKGVVSLAWAEEG
Sbjct: 197 HYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQKGVVSLAWAEEG 256

Query: 241 RTLFYTQSDENQRPYRQ-------NSVKDVSVFVENDPNFCVDVTSTKDGKFITV----- 300
           RTLFYTQSDENQRPYR        +  +DVSVFVENDPNFCVDVTSTKDGKFITV     
Sbjct: 257 RTLFYTQSDENQRPYRVFCTKVGCSDAEDVSVFVENDPNFCVDVTSTKDGKFITVNSNSR 316

Query: 301 -----YIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYVA 360
                YIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYVA
Sbjct: 317 TSSEVYIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYVA 376

Query: 361 RCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKHR 420
           RCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKHR
Sbjct: 377 RCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKHR 436

Query: 421 LEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSIIQ 480
           LEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSIIQ
Sbjct: 437 LEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSIIQ 496

Query: 481 QEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVISH 540
           QEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVISH
Sbjct: 497 QEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVISH 556

Query: 541 DGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRG 600
           DGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRG
Sbjct: 557 DGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRG 616

Query: 601 GGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAIN 660
           GGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAIN
Sbjct: 617 GGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAIN 676

Query: 661 MRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNISR 720
           MRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNISR
Sbjct: 677 MRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNISR 736

Query: 721 GSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYG 780
           GSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYG
Sbjct: 737 GSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYG 796

Query: 781 GCEETAYEYAFLIKVLGTSDHN 785
           GCEETAYEYAFLIKVLGTSDHN
Sbjct: 797 GCEETAYEYAFLIKVLGTSDHN 818

BLAST of MC09g1598.1 vs. NCBI nr
Match: XP_022977225.1 (uncharacterized protein LOC111477597 [Cucurbita maxima])

HSP 1 Score: 1368 bits (3541), Expect = 0.0
Identity = 672/803 (83.69%), Postives = 723/803 (90.04%), Query Frame = 0

Query: 1   MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH 60
           MALKSLL  KPKC  +K +L SSS  SSSLFSS C++R FSLPSESPP AKKVPF +SVH
Sbjct: 1   MALKSLL--KPKCFTRKAIL-SSSLASSSLFSSLCKERIFSLPSESPPTAKKVPFTHSVH 60

Query: 61  GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP 120
           G+TLQDP+HWM+NT DPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSR+P KVSTPP
Sbjct: 61  GITLQDPYHWMANTADPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRIPTKVSTPP 120

Query: 121 EPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAK 180
           EPWGPWFYYQYIP GKEYPVLCRRL N+K  WLKKL QFA+GN GK+EE VLLDWNEIAK
Sbjct: 121 EPWGPWFYYQYIPEGKEYPVLCRRLLNQKTNWLKKLTQFAKGNSGKQEE-VLLDWNEIAK 180

Query: 181 HYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQKGVVSLAWAEEG 240
            YGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQ+KDL SGL+IPK Q+GVVSLAWAEEG
Sbjct: 181 QYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQIKDLRSGLMIPKLQEGVVSLAWAEEG 240

Query: 241 RTLFYTQSDENQRPYR--------QNSVKDVSVFVENDPNFCVDVTSTKDGKFITV---- 300
           RTLFYTQ+DENQRPYR         N+ +DV VFVENDPN+CVD+TSTKDGKFITV    
Sbjct: 241 RTLFYTQADENQRPYRVFSTKLGFSNTEEDVLVFVENDPNYCVDITSTKDGKFITVNSNS 300

Query: 301 ------YIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYV 360
                 YIIDANN LSGLQRIHKRIPGIQYFLEHH GFFYILTNAPLEK GDC KE+YYV
Sbjct: 301 RTSSEVYIIDANNSLSGLQRIHKRIPGIQYFLEHHCGFFYILTNAPLEKKGDCLKEDYYV 360

Query: 361 ARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKH 420
           ARCRVEDIKS++WQD +LQS+DFSIQDMD+FSGHLVLFVNK GV MLC+INLPLD NHKH
Sbjct: 361 ARCRVEDIKSANWQDIVLQSQDFSIQDMDVFSGHLVLFVNKNGVPMLCSINLPLDANHKH 420

Query: 421 RLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSII 480
            LEIEKLDPWFFPLPSNSCSV+PGSNHDFMSSLYRVVLSSP+MPDL+VDYDMSKRVFSII
Sbjct: 421 CLEIEKLDPWFFPLPSNSCSVSPGSNHDFMSSLYRVVLSSPLMPDLIVDYDMSKRVFSII 480

Query: 481 QQEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVIS 540
           QQEEV+VKHDV+LKT  P+ L +E+VS A+NK  NF+N ES+  KDFSD+YCCERKEVIS
Sbjct: 481 QQEEVEVKHDVKLKTYQPNALGIEKVSDAQNKRENFENRESKTWKDFSDSYCCERKEVIS 540

Query: 541 HDGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVR 600
           HDGIR+PLTILYSP  F KGRS GVL GYGAYGE+LDKSWCP RLSLLDRGFVLAFAD+R
Sbjct: 541 HDGIRVPLTILYSPSTFQKGRSLGVLQGYGAYGEVLDKSWCPSRLSLLDRGFVLAFADIR 600

Query: 601 GGGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAI 660
           GGGGG DSSWHRSGSGLEKQNSI DFI CA FL+DN YVHKN+L SIGYSAGGLLVGAAI
Sbjct: 601 GGGGG-DSSWHRSGSGLEKQNSIQDFIFCANFLIDNGYVHKNRLASIGYSAGGLLVGAAI 660

Query: 661 NMRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNIS 720
           NM PDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQ+  QFESIL+YSPYDNIS
Sbjct: 661 NMHPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQIAMQFESILSYSPYDNIS 720

Query: 721 RGSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLY 780
           +GSCYPPMLVT+SFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNM+GGHFGEGGLY
Sbjct: 721 KGSCYPPMLVTASFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMVGGHFGEGGLY 780

Query: 781 GGCEETAYEYAFLIKVLGTSDHN 785
           GGCEETAY+YAFLIKVLGTSD +
Sbjct: 781 GGCEETAYDYAFLIKVLGTSDRD 798

BLAST of MC09g1598.1 vs. NCBI nr
Match: XP_023550805.1 (uncharacterized protein LOC111808835, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1366 bits (3535), Expect = 0.0
Identity = 673/803 (83.81%), Postives = 722/803 (89.91%), Query Frame = 0

Query: 1   MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH 60
           MALKSLL  KPKC  +K +L SSS  SSSLFSS C++R FSLPSESPPAAKKVPF +SVH
Sbjct: 10  MALKSLL--KPKCFTRKAIL-SSSIASSSLFSSLCKERIFSLPSESPPAAKKVPFTHSVH 69

Query: 61  GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP 120
           G+TLQDP+HWM+NT DPDLAD+LRRENLYA+AFMADTQILQRRLFSEMTSR+P KVSTPP
Sbjct: 70  GITLQDPYHWMANTADPDLADFLRRENLYADAFMADTQILQRRLFSEMTSRIPTKVSTPP 129

Query: 121 EPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAK 180
           EPWGPWFYYQYIP GKEYPVLCRRLQNEK  WLKKL QFA+GN GK+EE VLLDWNEIAK
Sbjct: 130 EPWGPWFYYQYIPEGKEYPVLCRRLQNEKTNWLKKLTQFAKGNSGKQEE-VLLDWNEIAK 189

Query: 181 HYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQKGVVSLAWAEEG 240
            YGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQ+KDL SGL+IPK Q+GVVSLAWAEEG
Sbjct: 190 QYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQIKDLRSGLMIPKLQEGVVSLAWAEEG 249

Query: 241 RTLFYTQSDENQRPYRQNSVK--------DVSVFVENDPNFCVDVTSTKDGKFITV---- 300
           RTLFYTQ+DENQRPYR  S K        DV VFVENDPN+CVD+TSTKDGKFITV    
Sbjct: 250 RTLFYTQADENQRPYRVFSTKLGFSDTEEDVLVFVENDPNYCVDITSTKDGKFITVNSNS 309

Query: 301 ------YIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYV 360
                 YIIDANN LSGLQRIHKRIPGIQYFLEHH GFFYILTNAPLEK GDCSKE+YYV
Sbjct: 310 RTSSEVYIIDANNSLSGLQRIHKRIPGIQYFLEHHRGFFYILTNAPLEKKGDCSKEDYYV 369

Query: 361 ARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKH 420
           A+CRVEDIKS++WQD +LQSEDFSIQDMD+FSGHLVLFVNK GV MLC+INLPLD NHKH
Sbjct: 370 AQCRVEDIKSANWQDTVLQSEDFSIQDMDVFSGHLVLFVNKNGVPMLCSINLPLDANHKH 429

Query: 421 RLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSII 480
           RLEIEKLDPWFFPLPSNSCSVAPGSNHDF SSLYRVVLSSPVMPDL+VDYDMSKRVFSII
Sbjct: 430 RLEIEKLDPWFFPLPSNSCSVAPGSNHDFTSSLYRVVLSSPVMPDLIVDYDMSKRVFSII 489

Query: 481 QQEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVIS 540
           QQEEV+VKHDV+LKT  PD  D+E+VS  +NK  NF+  ES+  KDFSD+YCCERKEVIS
Sbjct: 490 QQEEVEVKHDVKLKTYQPDASDIEKVS--QNKRENFETRESETWKDFSDSYCCERKEVIS 549

Query: 541 HDGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVR 600
           HDGIR+PLTILYSP  F KGRSPGVL GYGAYGE+LDKSWCP RLSLLDRGFVLAFAD+R
Sbjct: 550 HDGIRVPLTILYSPSTFQKGRSPGVLQGYGAYGEVLDKSWCPSRLSLLDRGFVLAFADIR 609

Query: 601 GGGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAI 660
           GGGGG DSSWHR GSGLEKQNSI DFI CA FL+DN YVHK++LGSIGYSAGGLLVGAAI
Sbjct: 610 GGGGG-DSSWHRCGSGLEKQNSIQDFIFCANFLIDNGYVHKDRLGSIGYSAGGLLVGAAI 669

Query: 661 NMRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNIS 720
           NM PDLF AAILKVPFLDICNTLLDPSLPLTILDYEEFGNP++  QFESIL+YSPYDNIS
Sbjct: 670 NMHPDLFGAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPEIAMQFESILSYSPYDNIS 729

Query: 721 RGSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLY 780
           +GSCYPPMLVT+SFRDARVGVWEAAKWVAKIRDTTC RCSTSAILKTNM+GGHFGEGGLY
Sbjct: 730 KGSCYPPMLVTASFRDARVGVWEAAKWVAKIRDTTCCRCSTSAILKTNMVGGHFGEGGLY 789

Query: 781 GGCEETAYEYAFLIKVLGTSDHN 785
           GGCEETAY+YAFLIKVLGTSD +
Sbjct: 790 GGCEETAYDYAFLIKVLGTSDRD 805

BLAST of MC09g1598.1 vs. NCBI nr
Match: XP_022957328.1 (uncharacterized protein LOC111458759 [Cucurbita moschata])

HSP 1 Score: 1358 bits (3514), Expect = 0.0
Identity = 671/803 (83.56%), Postives = 719/803 (89.54%), Query Frame = 0

Query: 1   MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH 60
           MALKSLL  KP    +  +L SS S SSSLFSS C++R FSLPSESPPAAKKVPF +SVH
Sbjct: 1   MALKSLL--KPT---RNSILCSSIS-SSSLFSSLCKERIFSLPSESPPAAKKVPFTHSVH 60

Query: 61  GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP 120
           G+TLQDP+HWM+NT DPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSR+  KVSTPP
Sbjct: 61  GITLQDPYHWMANTADPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRISTKVSTPP 120

Query: 121 EPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAK 180
           EPWGPWFYYQYIP GKEYPVLCRRLQNEK  WLKKL QFA+GN GK+EE VLLDWNEIAK
Sbjct: 121 EPWGPWFYYQYIPEGKEYPVLCRRLQNEKTNWLKKLTQFAKGNSGKQEE-VLLDWNEIAK 180

Query: 181 HYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQKGVVSLAWAEEG 240
            YGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQ+KDL SGL+IPK Q+GVVSLAWAEEG
Sbjct: 181 QYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQIKDLRSGLMIPKLQEGVVSLAWAEEG 240

Query: 241 RTLFYTQSDENQRPYRQNSVK--------DVSVFVENDPNFCVDVTSTKDGKFITV---- 300
           RTLFYTQ+DENQRPYR  S K        DV VFVENDPN+CVD+TSTKDGKFITV    
Sbjct: 241 RTLFYTQADENQRPYRVFSTKLGFSDTGEDVLVFVENDPNYCVDITSTKDGKFITVNSNS 300

Query: 301 ------YIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYV 360
                 YIIDANN LSGLQRIHKRIPGIQYFLEHH GFFYILTNAPLEK GDCSKE+YYV
Sbjct: 301 RTSSEVYIIDANNWLSGLQRIHKRIPGIQYFLEHHCGFFYILTNAPLEKKGDCSKEDYYV 360

Query: 361 ARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKH 420
           ARCRVEDIKS++WQD +LQS+DFSI DMD+FSGHLVLFVNK GV MLC+INLPLD NHKH
Sbjct: 361 ARCRVEDIKSANWQDIVLQSKDFSIHDMDVFSGHLVLFVNKNGVPMLCSINLPLDANHKH 420

Query: 421 RLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSII 480
           RLEIEKLDPWFFPLPSNSCSVAPGSNHDF SSLYRVVLSSPVMPDL+VDYDMSKRVFSII
Sbjct: 421 RLEIEKLDPWFFPLPSNSCSVAPGSNHDFTSSLYRVVLSSPVMPDLIVDYDMSKRVFSII 480

Query: 481 QQEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVIS 540
           QQEEV+VKHD++LKT  PD L +E+VS A+NK  NF+  ES+  KDFSD+YCCERKEVIS
Sbjct: 481 QQEEVEVKHDIKLKTYQPDALGIEKVSDAQNKRENFETRESETWKDFSDSYCCERKEVIS 540

Query: 541 HDGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVR 600
           HDGIR+PLTILYSP  F KGRSPGVL GYGAYGE+LDKSWCP RLSLLDRGFVLAFAD+R
Sbjct: 541 HDGIRVPLTILYSPSTFQKGRSPGVLQGYGAYGEVLDKSWCPSRLSLLDRGFVLAFADIR 600

Query: 601 GGGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAI 660
           GGGGG DSSWHR GSGL+KQNSI DFI CA FL+DN YVHKN+LGSIGYSAGGLLVGAAI
Sbjct: 601 GGGGG-DSSWHRCGSGLQKQNSIQDFIFCANFLIDNGYVHKNRLGSIGYSAGGLLVGAAI 660

Query: 661 NMRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNIS 720
           NM PDLF AAILKVPFLDICNTLLDPSLPLTILDYEEFGNP++  QFESIL+YSPYDNIS
Sbjct: 661 NMHPDLFGAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPEIAMQFESILSYSPYDNIS 720

Query: 721 RGSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLY 780
           +GSCYPPMLVT+SFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNM+GGHFGEGGLY
Sbjct: 721 KGSCYPPMLVTASFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMVGGHFGEGGLY 780

Query: 781 GGCEETAYEYAFLIKVLGTSDHN 785
           GGCEETAY+YAFLIKVLGTSD +
Sbjct: 781 GGCEETAYDYAFLIKVLGTSDRD 795

BLAST of MC09g1598.1 vs. NCBI nr
Match: XP_038892765.1 (protease 2 isoform X2 [Benincasa hispida])

HSP 1 Score: 1343 bits (3477), Expect = 0.0
Identity = 670/805 (83.23%), Postives = 716/805 (88.94%), Query Frame = 0

Query: 1   MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH 60
           MALKSLL  KPK SI K  L SS + SSS FSS C+   FSLPS+SPP AKK+PF +SVH
Sbjct: 1   MALKSLL--KPKSSITKFFLYSSIA-SSSRFSSSCKKPIFSLPSQSPPTAKKLPFTHSVH 60

Query: 61  GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP 120
           GVTLQD +HWMSNT DPDLADYLR+EN YAEAFM DTQILQ+RLFSEMTSR+PAKVSTPP
Sbjct: 61  GVTLQDSYHWMSNTHDPDLADYLRQENFYAEAFMGDTQILQQRLFSEMTSRIPAKVSTPP 120

Query: 121 EPWGPWFYYQYIPAGKEYPVLCRRLQNE--KIGWLKKLVQFARGNFGKEEEEVLLDWNEI 180
           EPWGPWFYYQYIP GKEYPVLCRRLQNE  K  WLKKL+QF +GNFGKEE+ VLLDWNEI
Sbjct: 121 EPWGPWFYYQYIPEGKEYPVLCRRLQNENEKSSWLKKLLQFVKGNFGKEEQ-VLLDWNEI 180

Query: 181 AKHYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQK-GVVSLAWA 240
           AK YGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQ+KDLGSG IIP+ QK GVVSLAWA
Sbjct: 181 AKQYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQIKDLGSGRIIPELQKEGVVSLAWA 240

Query: 241 EEGRTLFYTQSDENQRPYRQNSVK-------DVSVFVENDPNFCVDVTSTKDGKFITV-- 300
           EEGR LFYTQ+D NQRPYR    K       DVSVFVENDPN+CVD+TSTKDGKF+TV  
Sbjct: 241 EEGRMLFYTQADGNQRPYRVFCTKVGFKDTEDVSVFVENDPNYCVDITSTKDGKFVTVNS 300

Query: 301 --------YIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEY 360
                   YIIDA N +SGLQRIHKR+PGIQYFLEHH GFFYILTNAPLEKNGDC KE+Y
Sbjct: 301 NSRTSSEVYIIDAKNSVSGLQRIHKRVPGIQYFLEHHHGFFYILTNAPLEKNGDCLKEDY 360

Query: 361 YVARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNH 420
           YVARCRVEDIKS+D QD ILQSEDFSIQDMD+F GHLVLFVNK GV MLC+INLPLD N 
Sbjct: 361 YVARCRVEDIKSADQQDIILQSEDFSIQDMDLFGGHLVLFVNKNGVPMLCSINLPLDVND 420

Query: 421 KHRLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFS 480
           K  +EI+KLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDL+VDYDMS+RVFS
Sbjct: 421 KQCIEIKKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSRRVFS 480

Query: 481 IIQQEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEV 540
           IIQQEEV+VK  V+LKT LPD LD+ EVS A+NK  NFQNSESQ  KDFSD Y CERKEV
Sbjct: 481 IIQQEEVKVKPGVKLKTYLPDALDIGEVSDAQNKRENFQNSESQNWKDFSDVYYCERKEV 540

Query: 541 ISHDGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFAD 600
           ISHDGIRIPLTILYSP+NF KG+SPGVL GYGAYGEILDKSWCPYRLSLLDRGFVLAFAD
Sbjct: 541 ISHDGIRIPLTILYSPMNFQKGQSPGVLQGYGAYGEILDKSWCPYRLSLLDRGFVLAFAD 600

Query: 601 VRGGGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGA 660
           VRGG GGGDSSWHR GSGLEKQNSI DFISCA FLV+N Y+HK++LGSIGYSAGGLLVGA
Sbjct: 601 VRGGSGGGDSSWHRCGSGLEKQNSILDFISCANFLVNNGYIHKDRLGSIGYSAGGLLVGA 660

Query: 661 AINMRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDN 720
           AINM PDLFRAAILKVPFLD+CNTLLDP+LPLTILDYEEFG+PQ+PKQF SIL+YSPYDN
Sbjct: 661 AINMHPDLFRAAILKVPFLDVCNTLLDPTLPLTILDYEEFGSPQIPKQFRSILSYSPYDN 720

Query: 721 ISRGSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGG 780
           IS+GSCYPPMLVT+S RDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGG
Sbjct: 721 ISKGSCYPPMLVTASLRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGG 780

Query: 781 LYGGCEETAYEYAFLIKVLGTSDHN 785
           LYGGCEETAYEYAFLIKVLGTSDH+
Sbjct: 781 LYGGCEETAYEYAFLIKVLGTSDHD 801

BLAST of MC09g1598.1 vs. ExPASy TrEMBL
Match: A0A6J1D5U2 (Prolyl endopeptidase OS=Momordica charantia OX=3673 GN=LOC111017556 PE=3 SV=1)

HSP 1 Score: 1586 bits (4107), Expect = 0.0
Identity = 780/802 (97.26%), Postives = 782/802 (97.51%), Query Frame = 0

Query: 1   MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH 60
           MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH
Sbjct: 17  MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH 76

Query: 61  GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP 120
           GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP
Sbjct: 77  GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP 136

Query: 121 EPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAK 180
           EPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAK
Sbjct: 137 EPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAK 196

Query: 181 HYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQKGVVSLAWAEEG 240
           HYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQKGVVSLAWAEEG
Sbjct: 197 HYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQKGVVSLAWAEEG 256

Query: 241 RTLFYTQSDENQRPYRQ-------NSVKDVSVFVENDPNFCVDVTSTKDGKFITV----- 300
           RTLFYTQSDENQRPYR        +  +DVSVFVENDPNFCVDVTSTKDGKFITV     
Sbjct: 257 RTLFYTQSDENQRPYRVFCTKVGCSDAEDVSVFVENDPNFCVDVTSTKDGKFITVNSNSR 316

Query: 301 -----YIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYVA 360
                YIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYVA
Sbjct: 317 TSSEVYIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYVA 376

Query: 361 RCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKHR 420
           RCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKHR
Sbjct: 377 RCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKHR 436

Query: 421 LEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSIIQ 480
           LEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSIIQ
Sbjct: 437 LEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSIIQ 496

Query: 481 QEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVISH 540
           QEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVISH
Sbjct: 497 QEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVISH 556

Query: 541 DGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRG 600
           DGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRG
Sbjct: 557 DGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRG 616

Query: 601 GGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAIN 660
           GGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAIN
Sbjct: 617 GGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAIN 676

Query: 661 MRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNISR 720
           MRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNISR
Sbjct: 677 MRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNISR 736

Query: 721 GSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYG 780
           GSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYG
Sbjct: 737 GSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYG 796

Query: 781 GCEETAYEYAFLIKVLGTSDHN 785
           GCEETAYEYAFLIKVLGTSDHN
Sbjct: 797 GCEETAYEYAFLIKVLGTSDHN 818

BLAST of MC09g1598.1 vs. ExPASy TrEMBL
Match: A0A6J1ILQ3 (Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111477597 PE=3 SV=1)

HSP 1 Score: 1368 bits (3541), Expect = 0.0
Identity = 672/803 (83.69%), Postives = 723/803 (90.04%), Query Frame = 0

Query: 1   MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH 60
           MALKSLL  KPKC  +K +L SSS  SSSLFSS C++R FSLPSESPP AKKVPF +SVH
Sbjct: 1   MALKSLL--KPKCFTRKAIL-SSSLASSSLFSSLCKERIFSLPSESPPTAKKVPFTHSVH 60

Query: 61  GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP 120
           G+TLQDP+HWM+NT DPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSR+P KVSTPP
Sbjct: 61  GITLQDPYHWMANTADPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRIPTKVSTPP 120

Query: 121 EPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAK 180
           EPWGPWFYYQYIP GKEYPVLCRRL N+K  WLKKL QFA+GN GK+EE VLLDWNEIAK
Sbjct: 121 EPWGPWFYYQYIPEGKEYPVLCRRLLNQKTNWLKKLTQFAKGNSGKQEE-VLLDWNEIAK 180

Query: 181 HYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQKGVVSLAWAEEG 240
            YGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQ+KDL SGL+IPK Q+GVVSLAWAEEG
Sbjct: 181 QYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQIKDLRSGLMIPKLQEGVVSLAWAEEG 240

Query: 241 RTLFYTQSDENQRPYR--------QNSVKDVSVFVENDPNFCVDVTSTKDGKFITV---- 300
           RTLFYTQ+DENQRPYR         N+ +DV VFVENDPN+CVD+TSTKDGKFITV    
Sbjct: 241 RTLFYTQADENQRPYRVFSTKLGFSNTEEDVLVFVENDPNYCVDITSTKDGKFITVNSNS 300

Query: 301 ------YIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYV 360
                 YIIDANN LSGLQRIHKRIPGIQYFLEHH GFFYILTNAPLEK GDC KE+YYV
Sbjct: 301 RTSSEVYIIDANNSLSGLQRIHKRIPGIQYFLEHHCGFFYILTNAPLEKKGDCLKEDYYV 360

Query: 361 ARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKH 420
           ARCRVEDIKS++WQD +LQS+DFSIQDMD+FSGHLVLFVNK GV MLC+INLPLD NHKH
Sbjct: 361 ARCRVEDIKSANWQDIVLQSQDFSIQDMDVFSGHLVLFVNKNGVPMLCSINLPLDANHKH 420

Query: 421 RLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSII 480
            LEIEKLDPWFFPLPSNSCSV+PGSNHDFMSSLYRVVLSSP+MPDL+VDYDMSKRVFSII
Sbjct: 421 CLEIEKLDPWFFPLPSNSCSVSPGSNHDFMSSLYRVVLSSPLMPDLIVDYDMSKRVFSII 480

Query: 481 QQEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVIS 540
           QQEEV+VKHDV+LKT  P+ L +E+VS A+NK  NF+N ES+  KDFSD+YCCERKEVIS
Sbjct: 481 QQEEVEVKHDVKLKTYQPNALGIEKVSDAQNKRENFENRESKTWKDFSDSYCCERKEVIS 540

Query: 541 HDGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVR 600
           HDGIR+PLTILYSP  F KGRS GVL GYGAYGE+LDKSWCP RLSLLDRGFVLAFAD+R
Sbjct: 541 HDGIRVPLTILYSPSTFQKGRSLGVLQGYGAYGEVLDKSWCPSRLSLLDRGFVLAFADIR 600

Query: 601 GGGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAI 660
           GGGGG DSSWHRSGSGLEKQNSI DFI CA FL+DN YVHKN+L SIGYSAGGLLVGAAI
Sbjct: 601 GGGGG-DSSWHRSGSGLEKQNSIQDFIFCANFLIDNGYVHKNRLASIGYSAGGLLVGAAI 660

Query: 661 NMRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNIS 720
           NM PDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQ+  QFESIL+YSPYDNIS
Sbjct: 661 NMHPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQIAMQFESILSYSPYDNIS 720

Query: 721 RGSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLY 780
           +GSCYPPMLVT+SFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNM+GGHFGEGGLY
Sbjct: 721 KGSCYPPMLVTASFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMVGGHFGEGGLY 780

Query: 781 GGCEETAYEYAFLIKVLGTSDHN 785
           GGCEETAY+YAFLIKVLGTSD +
Sbjct: 781 GGCEETAYDYAFLIKVLGTSDRD 798

BLAST of MC09g1598.1 vs. ExPASy TrEMBL
Match: A0A6J1GZW2 (Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111458759 PE=3 SV=1)

HSP 1 Score: 1358 bits (3514), Expect = 0.0
Identity = 671/803 (83.56%), Postives = 719/803 (89.54%), Query Frame = 0

Query: 1   MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH 60
           MALKSLL  KP    +  +L SS S SSSLFSS C++R FSLPSESPPAAKKVPF +SVH
Sbjct: 1   MALKSLL--KPT---RNSILCSSIS-SSSLFSSLCKERIFSLPSESPPAAKKVPFTHSVH 60

Query: 61  GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP 120
           G+TLQDP+HWM+NT DPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSR+  KVSTPP
Sbjct: 61  GITLQDPYHWMANTADPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRISTKVSTPP 120

Query: 121 EPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAK 180
           EPWGPWFYYQYIP GKEYPVLCRRLQNEK  WLKKL QFA+GN GK+EE VLLDWNEIAK
Sbjct: 121 EPWGPWFYYQYIPEGKEYPVLCRRLQNEKTNWLKKLTQFAKGNSGKQEE-VLLDWNEIAK 180

Query: 181 HYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQKGVVSLAWAEEG 240
            YGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQ+KDL SGL+IPK Q+GVVSLAWAEEG
Sbjct: 181 QYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQIKDLRSGLMIPKLQEGVVSLAWAEEG 240

Query: 241 RTLFYTQSDENQRPYRQNSVK--------DVSVFVENDPNFCVDVTSTKDGKFITV---- 300
           RTLFYTQ+DENQRPYR  S K        DV VFVENDPN+CVD+TSTKDGKFITV    
Sbjct: 241 RTLFYTQADENQRPYRVFSTKLGFSDTGEDVLVFVENDPNYCVDITSTKDGKFITVNSNS 300

Query: 301 ------YIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYV 360
                 YIIDANN LSGLQRIHKRIPGIQYFLEHH GFFYILTNAPLEK GDCSKE+YYV
Sbjct: 301 RTSSEVYIIDANNWLSGLQRIHKRIPGIQYFLEHHCGFFYILTNAPLEKKGDCSKEDYYV 360

Query: 361 ARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKH 420
           ARCRVEDIKS++WQD +LQS+DFSI DMD+FSGHLVLFVNK GV MLC+INLPLD NHKH
Sbjct: 361 ARCRVEDIKSANWQDIVLQSKDFSIHDMDVFSGHLVLFVNKNGVPMLCSINLPLDANHKH 420

Query: 421 RLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSII 480
           RLEIEKLDPWFFPLPSNSCSVAPGSNHDF SSLYRVVLSSPVMPDL+VDYDMSKRVFSII
Sbjct: 421 RLEIEKLDPWFFPLPSNSCSVAPGSNHDFTSSLYRVVLSSPVMPDLIVDYDMSKRVFSII 480

Query: 481 QQEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVIS 540
           QQEEV+VKHD++LKT  PD L +E+VS A+NK  NF+  ES+  KDFSD+YCCERKEVIS
Sbjct: 481 QQEEVEVKHDIKLKTYQPDALGIEKVSDAQNKRENFETRESETWKDFSDSYCCERKEVIS 540

Query: 541 HDGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVR 600
           HDGIR+PLTILYSP  F KGRSPGVL GYGAYGE+LDKSWCP RLSLLDRGFVLAFAD+R
Sbjct: 541 HDGIRVPLTILYSPSTFQKGRSPGVLQGYGAYGEVLDKSWCPSRLSLLDRGFVLAFADIR 600

Query: 601 GGGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAI 660
           GGGGG DSSWHR GSGL+KQNSI DFI CA FL+DN YVHKN+LGSIGYSAGGLLVGAAI
Sbjct: 601 GGGGG-DSSWHRCGSGLQKQNSIQDFIFCANFLIDNGYVHKNRLGSIGYSAGGLLVGAAI 660

Query: 661 NMRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNIS 720
           NM PDLF AAILKVPFLDICNTLLDPSLPLTILDYEEFGNP++  QFESIL+YSPYDNIS
Sbjct: 661 NMHPDLFGAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPEIAMQFESILSYSPYDNIS 720

Query: 721 RGSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLY 780
           +GSCYPPMLVT+SFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNM+GGHFGEGGLY
Sbjct: 721 KGSCYPPMLVTASFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMVGGHFGEGGLY 780

Query: 781 GGCEETAYEYAFLIKVLGTSDHN 785
           GGCEETAY+YAFLIKVLGTSD +
Sbjct: 781 GGCEETAYDYAFLIKVLGTSDRD 795

BLAST of MC09g1598.1 vs. ExPASy TrEMBL
Match: A0A0A0KQZ9 (Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_5G606880 PE=3 SV=1)

HSP 1 Score: 1342 bits (3473), Expect = 0.0
Identity = 655/796 (82.29%), Postives = 710/796 (89.20%), Query Frame = 0

Query: 1   MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRS---FSLPSESPPAAKKVPFKY 60
           MALK+LL  KPK SI K  L S S      FSSFC+ +    FS PS+SPP+ KK+PF +
Sbjct: 1   MALKTLL--KPKSSITKFFLSSLS------FSSFCKQQQQPIFSFPSQSPPSPKKLPFTH 60

Query: 61  SVHGVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVS 120
           SVHGV LQDP+HWMSNT DPD ADYLR+ENLYAEAFMADTQ+LQR+LFSEMTSR+PAKVS
Sbjct: 61  SVHGVKLQDPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVS 120

Query: 121 TPPEPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNE 180
           TPPEPWGPWFYYQYIP GKEYPVLCRRLQNEK  W +K++ F +GN GKEE+ VLLDWNE
Sbjct: 121 TPPEPWGPWFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQ-VLLDWNE 180

Query: 181 IAKHYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQK-GVVSLAW 240
           IAK YGYVHVGTCR+SPDHNFLAYTVDITG+EHFMLQ+KDL +GLIIPK QK GVVSLAW
Sbjct: 181 IAKQYGYVHVGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLIIPKLQKEGVVSLAW 240

Query: 241 AEEGRTLFYTQSDENQRPYRQ-------NSVKDVSVFVENDPNFCVDVTSTKDGKFITVY 300
           AEEGR LFYTQ+DENQRPYR        N  +DVSVFVENDPN+CVD+TSTKDGKFITVY
Sbjct: 241 AEEGRVLFYTQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFITVY 300

Query: 301 IIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYVARCRVED 360
           IIDANN L GLQRIH+RIPGIQYFLEHH GFFYILTNAPLEKNG CS+E+YYVARCRVED
Sbjct: 301 IIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGCSEEDYYVARCRVED 360

Query: 361 IKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKHRLEIEKL 420
           IKS+DWQD +LQSEDFSIQDMDIFSGHLVLFVNK GVSMLC+IN PLD +H H LEI+KL
Sbjct: 361 IKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFPLDADHNHHLEIDKL 420

Query: 421 DPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSIIQQEEVQV 480
           DPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDL+VDYDMSKR+FSIIQQEEV+V
Sbjct: 421 DPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRIFSIIQQEEVKV 480

Query: 481 KHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVISHDGIRIP 540
           +HDV+LKT LPD LD EEVS  ++K  NFQN ESQ  KDFS+AY CER EV SHDGIRIP
Sbjct: 481 QHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFCERIEVKSHDGIRIP 540

Query: 541 LTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGD 600
           LTILYSP+ F KG+SPG+L GYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGD
Sbjct: 541 LTILYSPMTFKKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGD 600

Query: 601 SSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAINMRPDLF 660
           SSWHR GSGLEK NSIHDFISCA FL+ N YVHK++LGSIGYSAGGLLVGAAINM P+LF
Sbjct: 601 SSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGGLLVGAAINMHPNLF 660

Query: 661 RAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNISRGSCYPP 720
           RAAILKVPFLDICNTLLDPSLPLT+LDYEEFGNPQ+ KQFESIL+YSPYDNIS+G+CYP 
Sbjct: 661 RAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYDNISKGTCYPS 720

Query: 721 MLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYGGCEETA 780
           MLVT+SF DARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYGGCEE A
Sbjct: 721 MLVTASFHDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYGGCEEMA 780

Query: 781 YEYAFLIKVLGTSDHN 785
           YEYAFLIKVL T DH+
Sbjct: 781 YEYAFLIKVLRTPDHD 787

BLAST of MC09g1598.1 vs. ExPASy TrEMBL
Match: A0A1S3BF53 (Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103489202 PE=3 SV=1)

HSP 1 Score: 1332 bits (3447), Expect = 0.0
Identity = 652/805 (80.99%), Postives = 711/805 (88.32%), Query Frame = 0

Query: 1   MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRS--FSLPSESPPAAKKVPFKYS 60
           MALKSLL  KPK SI K  L S S      FSSFC+ +   FS P +SPP+ KK+PF +S
Sbjct: 13  MALKSLL--KPKSSITKFFLSSLS------FSSFCKQQQPIFSFPPQSPPSPKKLPFTHS 72

Query: 61  VHGVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVST 120
           VHGVTLQDP+HWMSNT DPDL+DYLR+ENLYAEAFMADT++LQR+LFSEMT R+P+KVST
Sbjct: 73  VHGVTLQDPYHWMSNTHDPDLSDYLRQENLYAEAFMADTRVLQRQLFSEMTGRIPSKVST 132

Query: 121 PPEPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEI 180
           PPEPWGPWFYYQYIP GKEYPVLCRRLQNEK  W KK++QF +GNFGKEE+ VLLDWNEI
Sbjct: 133 PPEPWGPWFYYQYIPDGKEYPVLCRRLQNEKSSWFKKILQFGKGNFGKEEQ-VLLDWNEI 192

Query: 181 AKHYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIPKSQK-GVVSLAWA 240
           AK YGYVHVGTCRVSPDHNFLAYTVDITG EHFMLQ+KDL +GLIIPK QK GVVSLAWA
Sbjct: 193 AKRYGYVHVGTCRVSPDHNFLAYTVDITGDEHFMLQIKDLRNGLIIPKLQKEGVVSLAWA 252

Query: 241 EEGRTLFYTQSDENQRPYRQ-------NSVKDVSVFVENDPNFCVDVTSTKDGKFITV-- 300
           EEGR LFYTQ+DENQRPYR        N  +DVSVFVENDPN+CVD+TSTKDGKF+TV  
Sbjct: 253 EEGRMLFYTQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVDITSTKDGKFVTVNS 312

Query: 301 --------YIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEY 360
                   YIIDANN L GLQRIH+RIPGIQYFLEHH GFFYILTNAPLEKN DC +E+Y
Sbjct: 313 NSRTSSEVYIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNVDCLEEDY 372

Query: 361 YVARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNH 420
           YVARCRVEDIKS+DWQD +LQSEDFSIQDMDIFSGHLVLFVNK GVSMLC+INLPLD + 
Sbjct: 373 YVARCRVEDIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINLPLDADD 432

Query: 421 KHRLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFS 480
            H LEIEKLDPWFFPLPSNSCSVAPGSNHDFMSS YRVVLSSPVMPDL+VDYDMSKR FS
Sbjct: 433 NHHLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSSYRVVLSSPVMPDLIVDYDMSKRTFS 492

Query: 481 IIQQEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEV 540
           IIQQEEV+V+HDV+LKT LPD LDV+EVS  +NK  NFQN +SQ  KDFS+AYCCER EV
Sbjct: 493 IIQQEEVKVQHDVELKTNLPDTLDVQEVSDTQNKRENFQNCDSQNWKDFSEAYCCERIEV 552

Query: 541 ISHDGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFAD 600
            SHDG+ IPLTILY+P+ F KG+SPGVL GYGAYGEILDKSWCPYRLSLLDRGFVLAFAD
Sbjct: 553 TSHDGVGIPLTILYTPMTFQKGQSPGVLQGYGAYGEILDKSWCPYRLSLLDRGFVLAFAD 612

Query: 601 VRGGGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGA 660
           VRGGGGGGDSSWHRSG+GLEK NSIHDF+SCA FL++N YVHK++LGSIGYSAGGLLVGA
Sbjct: 613 VRGGGGGGDSSWHRSGNGLEKPNSIHDFVSCANFLINNGYVHKDRLGSIGYSAGGLLVGA 672

Query: 661 AINMRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDN 720
           AINM P+LFRAAILKVPFLDICNTLLDPSLPLT+LDYEEFGNPQ+ KQFESIL+YSPY+N
Sbjct: 673 AINMHPNLFRAAILKVPFLDICNTLLDPSLPLTVLDYEEFGNPQIQKQFESILSYSPYEN 732

Query: 721 ISRGSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGG 780
           IS+GSCYP MLVT+SF DARVGVWEAAKWVAKIRDTTCSRCS+SAILKTNMLGGHFGEGG
Sbjct: 733 ISKGSCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSRCSSSAILKTNMLGGHFGEGG 792

Query: 781 LYGGCEETAYEYAFLIKVLGTSDHN 785
           LYGGCEE AYEYAFLIKVL T DH+
Sbjct: 793 LYGGCEEMAYEYAFLIKVLRTPDHD 808

BLAST of MC09g1598.1 vs. TAIR 10
Match: AT1G69020.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 872.8 bits (2254), Expect = 2.0e-253
Identity = 431/774 (55.68%), Postives = 544/774 (70.28%), Query Frame = 0

Query: 31  FSSFC---RDRSFSLPSESPPAAKKVPFKYSVHGVTLQDPFHWMSNTDDPDLADYLRREN 90
           FS+ C   R  S S+P+E+PP  KK+PF  S HG+T QDPFHWM NTDD D  D+L+REN
Sbjct: 21  FSTKCFVGRTSSLSVPTEAPPVPKKIPFAISSHGITRQDPFHWMKNTDDTDFVDFLKREN 80

Query: 91  LYAEAFMADTQILQRRLFSEMTSRMPAKVSTPPEPWGPWFYYQYIPAGKEYPVLCRRLQN 150
            Y++AFMADT+ L+R LFSEM +R+P ++ TPPE WG W Y QYIP GKEYP+LCRRL+ 
Sbjct: 81  SYSQAFMADTETLRRDLFSEMKTRIPEEIFTPPERWGQWLYRQYIPKGKEYPLLCRRLEK 140

Query: 151 EKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAKHYGYVHVGTCRVSPDHNFLAYTVDITG 210
            K  WL        G F  EEEEV+LDWN+IA+ +GYVHVG CRVSPDHN+LAYTVD  G
Sbjct: 141 GKTNWLS-------GLFRGEEEEVVLDWNQIAEQFGYVHVGVCRVSPDHNYLAYTVDPEG 200

Query: 211 SEHFMLQVKDLGSGLIIPKSQKGVVSLAWAEEGRTLFYTQSDENQRPYR-------QNSV 270
                                          +G TLFYT +DENQRP+R        +  
Sbjct: 201 -------------------------------DGITLFYTVTDENQRPHRVVVTNVESDGR 260

Query: 271 KDVSVFVENDPNFCVDVTSTKDGKFIT----------VYIIDANNPLSGLQRIHKRIPGI 330
            D  VF E D +FCVD+T+TKDGKF+T          VYI++A+ P++GLQR  +R+PG+
Sbjct: 261 DDAVVFTERDSSFCVDITTTKDGKFVTINSNSRTSSEVYIVNADKPMAGLQRTRERVPGV 320

Query: 331 QYFLEHHFGFFYILTNAPLEKNGDCSKEEYYVARCRVEDIKSSDWQDAILQSEDFSIQDM 390
           Q FLEHH GFFYILTN+P     + S E YY+ RC VE+I++SDWQ      +D  IQDM
Sbjct: 321 QCFLEHHNGFFYILTNSPSNAISEWSGEGYYLTRCLVEEIEASDWQTVFRPDDDVVIQDM 380

Query: 391 DIFSGHLVLFVNKMGVSMLCAINLPLDTNHKHRLEIEKLDPWFFPLPSNSCSVAPGSNHD 450
           D+F+ +LVL++NK G+ MLC+I++P+  N KH   ++ L PW+FPLP +SCSVAPGSNHD
Sbjct: 381 DMFNDYLVLYLNKKGLPMLCSIDMPIKANTKH---MDDLVPWYFPLPVDSCSVAPGSNHD 440

Query: 451 FMSSLYRVVLSSPVMPDLVVDYDMSKRVFSIIQQEEVQVKHDVQLKTCLPDELDVEEVST 510
           F SS+YRVVLSSPV+PD +VDYD+S+R+FSI+QQE   V +    K     +   E    
Sbjct: 441 FQSSIYRVVLSSPVIPDTIVDYDVSRRLFSIVQQEGGVVDNSDSSKPWYTADRSTENNGQ 500

Query: 511 AENKIANFQNS--ESQISK--DFSDAYCCERKEVISHDGIRIPLTILYSPVNFHKGRSPG 570
             ++ +  ++   +S++ K  D SD Y CER+EV SHDG+ +PLTILYS   + K  SPG
Sbjct: 501 LNDRTSEGEDGQLDSRMPKWEDLSDTYVCERQEVSSHDGVEVPLTILYSREAWKKSESPG 560

Query: 571 VLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRSGSGLEKQNSIH 630
           +L GYGAYGE+LDKSWC  RLS+LDRG+V+AFADVR GGG G+ SWH+SG+   KQNSI 
Sbjct: 561 MLIGYGAYGEVLDKSWCTNRLSMLDRGWVIAFADVR-GGGSGEFSWHKSGTRSLKQNSIQ 620

Query: 631 DFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAINMRPDLFRAAILKVPFLDICNTLL 690
           DFI  AK+LV+  YVH++ L ++GYSAG +L  AA+NM P LF+A ILKVPF+D+ NTL 
Sbjct: 621 DFIYSAKYLVEKGYVHRHHLAAVGYSAGAILPAAAMNMHPSLFQAVILKVPFVDVLNTLS 680

Query: 691 DPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNISRGSCYPPMLVTSSFRDARVGVWEA 750
           DP+LPLT+LD+EEFGNP     F SIL+YSPYD I +  CYP MLVT+SF D+RVGVWE 
Sbjct: 681 DPNLPLTLLDHEEFGNPDNQTDFGSILSYSPYDKIRKDVCYPSMLVTTSFHDSRVGVWEG 740

Query: 751 AKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVLG 781
           AKWVAKIRD+TC  CS + ILKTNM GGHFGEGG Y  CEETA++YAFL+KV+G
Sbjct: 741 AKWVAKIRDSTCHDCSRAVILKTNMNGGHFGEGGRYAQCEETAFDYAFLLKVMG 752

BLAST of MC09g1598.1 vs. TAIR 10
Match: AT5G66960.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 529.6 bits (1363), Expect = 4.2e-150
Identity = 312/778 (40.10%), Postives = 443/778 (56.94%), Query Frame = 0

Query: 43  PSESPPAAKKVPFK---YSVHGVTLQDPFHWMSNTDDP----DLADYLRRENLYAEAFMA 102
           P   PPA  K P K   ++ H  T +DP+ WMS  +D      +  Y+ +E  Y EA +A
Sbjct: 32  PPPPPPALPKPPKKPQSFTFHDATWEDPYSWMSKLEDKVAMRHMDIYMEQEEKYTEAVLA 91

Query: 103 DTQILQRRLFSEMTSRMPAKVSTPPEPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKK 162
           DT  +Q +L SEM SR+  ++STPP  WGPW YY+ +  GK+YPVLCRRL +    ++  
Sbjct: 92  DTDRIQTKLQSEMASRLSFELSTPPLRWGPWLYYRRVEEGKQYPVLCRRLASLHEEFISH 151

Query: 163 LVQFARGNF--GKEEEEVLLDWNEIAKHY-GYVHVGTCRVSPDHNFLAYTVDITGSEHFM 222
               A  ++  GK  E+ LLD+N+ A+ + GY +     +SPDH FLAYT+    +++F 
Sbjct: 152 KSPAAGFDYTSGKRIEQKLLDYNQEAERFGGYAYEEMSEISPDHKFLAYTMYDKDNDYFK 211

Query: 223 LQVKDLGSGLIIPKSQKGVVS-LAWAEEGRTLFYTQSDENQRPYR------QNSVKDVSV 282
           L V++L SG +  K     VS +AWA+ G+ L Y  +D+ +RP R       ++ +DV +
Sbjct: 212 LCVRNLNSGALCSKPHADRVSNIAWAKNGQALLYVVTDQKKRPCRIYCSTIGSTDEDVLL 271

Query: 283 FVENDPNFCVDVTSTKDGKFIT----------VYIIDANNPLSGLQRIHKRIPGIQYFLE 342
             E + N  V++  TKD  F+T          V++I+A +P SGL  + +        +E
Sbjct: 272 HEEFEGNVHVNIRHTKDFHFVTVNTFSTTFSKVFLINAADPFSGLALVWEHNAPAHCIIE 331

Query: 343 HHFGFFYILTNAPLEKNGDCSKEEYYVARCRVEDIKSSD-WQDAILQSEDFSIQDMDIFS 402
           HH GF Y+ TNA    N   + + +Y+ R  V        W+   +   +  I+D+D   
Sbjct: 332 HHQGFLYLFTNA---SNDGGTVDHHYLLRSPVHFSSCQRIWETVFINDPELIIEDVDFCK 391

Query: 403 GHLVLFVNKMGVSMLCAINLPLDTNHKHRLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSS 462
            HL L V +M    +C ++LPL T  +  + +  + P + PLP +   + PG+N+DF S 
Sbjct: 392 KHLSLIVKEMQSFKICVVDLPLKTK-RVPVHLRDIKPRYLPLPKHVSQIFPGTNYDFNSP 451

Query: 463 LYRVVLSSPVMPDLVVDYDMSKRVFSIIQQ-----EEVQVKHDVQLKTCLPD-------- 522
             R  +SS VMPD VVDYD+    ++I+QQ     E  +V +     T  P+        
Sbjct: 452 TMRFTISSLVMPDAVVDYDLLNGKWNIVQQQNMLHERTRVLYGTANSTESPNIPSGTRTV 511

Query: 523 ELDVEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVISHDGIRIPLTILYSPVNFHK 582
             D E+ +TAEN           +  D ++ Y C+  EV SHDG  +PL+I+YS     +
Sbjct: 512 SFDTED-TTAEN---------DNLWNDLTEFYACDYHEVSSHDGAMVPLSIVYSRAQKEE 571

Query: 583 GRSPGVLHGYGAYGEILDKSWCPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRSGSGLEK 642
            + PG+LH +GAYGE+LDK W     SLLDRG+VLA+ADVRGGGG G   WH+ G G +K
Sbjct: 572 NQKPGLLHVHGAYGEMLDKRWRSELKSLLDRGWVLAYADVRGGGGKG-KKWHQDGRGAKK 631

Query: 643 QNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAINMRPDLFRAAILKVPFLDI 702
            NSI D+I CAK+LV+N+ V +N+L   GYSAGGL+V +AIN  PDLF+AA+LKVPFLD 
Sbjct: 632 LNSIKDYIQCAKYLVENNIVEENKLAGWGYSAGGLVVASAINHCPDLFQAAVLKVPFLDP 691

Query: 703 CNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNISRGSCYPPMLVTSSFRDARV 762
            +TL+ P LPLT  DYEEFG P     F +I  YSPYDNI +   YP +LVTSSF + R 
Sbjct: 692 THTLIYPILPLTAEDYEEFGYPGDINDFHAIREYSPYDNIPKDVLYPAVLVTSSF-NTRF 751

Query: 763 GVWEAAKWVAKIRDTTCSRCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVL 780
           GVWEAAKWVA++RD T +      +L    L     E   +   +E+A E AFLIK++
Sbjct: 752 GVWEAAKWVARVRDNTFNDPERPVLLN---LTTDIVEENRFLQTKESALEIAFLIKMM 790

BLAST of MC09g1598.1 vs. TAIR 10
Match: AT1G50380.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 334.7 bits (857), Expect = 2.0e-91
Identity = 231/757 (30.52%), Postives = 362/757 (47.82%), Query Frame = 0

Query: 44  SESPPAAKKVPFKYSVHGVTLQDPFHWM--SNTDDPDLADYLRRENLYAEAFMADTQILQ 103
           S SPP AKKV     + G    D ++W+   +  +PD+  YLR EN Y +  M+ T+  +
Sbjct: 4   SRSPPVAKKVEHVMEMFGDVRVDNYYWLRDDSRTNPDMLSYLREENHYTDFVMSGTKQFE 63

Query: 104 RRLFSEMTSRMPAKVSTPPEPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFAR 163
            +LF+E+  R+     + P   GP++YY+    GKEY   CRRL  +             
Sbjct: 64  NQLFAEIRGRIKEDDISAPLRKGPYYYYEKNLQGKEYIQHCRRLITDNKAEPSVYDTMPT 123

Query: 164 GNFGKEEEEVLLDWNEIAKHYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSG 223
           G      E V+LD N  A+ + Y  +G  + SPDH  +AY  D  G E + + V D  + 
Sbjct: 124 GP-DAPPEHVILDENTKAQEHDYYRIGAFKASPDHKLVAYAEDTKGDEIYTVNVIDSEAL 183

Query: 224 LIIPKSQKGVVS-LAWAEEGRTLFYTQSDENQRPYR-------QNSVKDVSVFVENDPNF 283
             + +  KG+ S L WA     L+ T  DE  RP +            DV ++ E D  F
Sbjct: 184 KPVGQQLKGLTSYLEWAGNDALLYITM-DEILRPDKVWLHKLGTEQSSDVCLYHEKDDMF 243

Query: 284 CVDVTSTKDGKFI----------TVYIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYI 343
            +++ +++  K++           V+ +D +    GL+ +  R+ GI   + H    F+I
Sbjct: 244 SLELHASESHKYLFVASESKTTRFVFSLDVSKTQDGLRVLTPRVDGIDSSVSHRGNHFFI 303

Query: 344 LTNAPLEKNGDCSKEEYYVARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNK 403
              +    N +       +  C V+D  +S     +   E   IQ++ +F  HL +F  +
Sbjct: 304 QRRSTEFYNSE-------LIACPVDD--TSKTTVLLPHRESVKIQEIQLFRDHLAVFERE 363

Query: 404 MGVSMLCAINLPLDTNHKHRLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSP 463
            G+  +    LP +      L+  +   +  P+ S        +  +F S + R    S 
Sbjct: 364 NGLQKITVHRLPAEGQPLEGLQGGRNVSFVDPVYS-----VDSTESEFSSRVLRFKYCSM 423

Query: 464 VMPDLVVDYDMSKRVFSIIQQEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSES 523
             P  V DYDM                           +     V   +  +  F  S  
Sbjct: 424 KTPPSVYDYDM---------------------------DSGTSVVKKIDTVLGGFDASN- 483

Query: 524 QISKDFSDAYCCERKEVISHDGIRIPLTILYS-PVNFHKGRSPGVLHGYGAYGEILDKSW 583
                    Y  ERK V + DG +IP++I+Y+  +    G  P +L+GYG+Y   +D  +
Sbjct: 484 ---------YVTERKWVAASDGTQIPMSIVYNKKLAKLDGSDPLLLYGYGSYEISVDPYF 543

Query: 584 CPYRLSLLDRGFVLAFADVRGGGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVH 643
              RLSLLDRGF    A VRGGG  G   W+ +G  L+K+N+  DFI+CA+ L++  Y  
Sbjct: 544 KASRLSLLDRGFTFVIAHVRGGGEMG-RQWYENGKLLKKKNTFTDFIACAERLIELKYCS 603

Query: 644 KNQLGSIGYSAGGLLVGAAINMRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGN 703
           K +L   G SAGGLL+GA +NMRPDLF+  I  VPF+D+  T+LDP++PLT  ++EE+G+
Sbjct: 604 KEKLCMEGRSAGGLLMGAVVNMRPDLFKVVIAGVPFVDVLTTMLDPTIPLTTSEWEEWGD 663

Query: 704 PQLPKQFESILNYSPYDNISRGSCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCS 763
           P+  + +  + +YSP DN++  + YP MLVT+   D RV   E  KWVAK+R+      +
Sbjct: 664 PRKEEFYFYMKSYSPVDNVTAQN-YPNMLVTAGLNDPRVMYSEPGKWVAKLREMKTD--N 703

Query: 764 TSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVL 780
              + K  +  GHF + G +   +E A+ +AF++KVL
Sbjct: 724 NVLLFKCELGAGHFSKSGRFEKLQEDAFTFAFMMKVL 703

BLAST of MC09g1598.1 vs. TAIR 10
Match: AT1G20380.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 132.9 bits (333), Expect = 1.1e-30
Identity = 172/792 (21.72%), Postives = 311/792 (39.27%), Query Frame = 0

Query: 47  PPAAKKVPFKYSVHGVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFS 106
           P A +        HGV + DP+ W+ + D  ++ +++ ++   +++ +   +  + +L  
Sbjct: 14  PTARRDESVVEDYHGVKVSDPYRWLEDPDAEEVKEFVEKQVQLSDSVLKSCE-TKEKLHD 73

Query: 107 EMTSRMP-AKVSTPPEPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFG 166
           + T  +   +  TP +    +FY+                     G   + V   + +  
Sbjct: 74  KFTKFIDYPRFDTPFKRGNSYFYF------------------HNSGLQAQSVLHVQDDL- 133

Query: 167 KEEEEVLLDWNEIAKHYGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGLIIP 226
           + E E+LLD N ++   G V + T  +S D  +LAY +  +GS+   ++V  +    + P
Sbjct: 134 ESEAEILLDPNTLSDD-GTVSLNTLSISEDAKYLAYGLSSSGSDWVTIKVMKIEDKKVEP 193

Query: 227 KSQKGV--VSLAWAEEGRTLFYTQ-------------SDENQRPYRQ--------NSVKD 286
            S   V    + W  +G+  FY++             ++ N   Y +        +  +D
Sbjct: 194 DSLSWVKFSGITWTHDGKGFFYSRYPAPREGEKIDAGTETNSNLYHELYYHFLGTDQSED 253

Query: 287 VSVFVEND-PNFCVDVTSTKDGKFITVYIIDANNP---------------LSGLQRIHKR 346
           V  + + D P        T DGK++ + I +  +P               L G +  +  
Sbjct: 254 VLCWRDQDNPKHMFGSKVTDDGKYLIMSIEEGCDPVNKVYHCDLSLLPKGLEGFRGSNTL 313

Query: 347 IPGIQYFLEHHFGFFYILTNAPLEKNGDCSKEEYYVARCRVEDIKSSDWQDAILQSEDFS 406
           +P ++  ++     +  + N         +K+       RV+  + S W D I + E   
Sbjct: 314 LPFVK-LIDTFDAQYIAIANDETLFTFLTNKDAPKYKVVRVDLKEPSSWTDVIAEHE--- 373

Query: 407 IQDMDIFSGHLVLFVNKMGVSMLCAINLPLDTNHKHRLEIE--KLDPWFFPLPSNSCSVA 466
               D+ S    +  +++ VS +  +        KH L+I   K       LP +  SV 
Sbjct: 374 ---KDVLSTASAVNGDQLVVSYMSDV--------KHILQIRDLKSGSLLHGLPVDIGSVC 433

Query: 467 PGSNHDFMSSLYRVVLSSPVMPDLVVDYDMSKRVFSIIQQEEVQVKHDVQLKTCLPDELD 526
            G       + +    +S + P ++   D+S       +  EV V  ++           
Sbjct: 434 -GVFARRKDTTFFFRFTSFLTPGVIYICDLSH------EAPEVTVFREI----------- 493

Query: 527 VEEVSTAENKIANFQNSESQISKDFSDAYCCERKEVISHDGIRIPLTILYSPVNFHKGRS 586
                     +  F  +  Q+++ F            S DG  IP+ I+        G  
Sbjct: 494 ---------GVPGFDRTAFQVTQVFYP----------SKDGTDIPMFIVARKDIKLDGSH 553

Query: 587 PGVLHGYGAYGEILDKSWCPYRLSL-LDRGFVLAFADVRGGGGGGDSSWHRSGSGLEKQN 646
           P +L+ YG +   +   +   R+ L    G V  FA++RGGG  G+  WH+SG+   KQN
Sbjct: 554 PCLLYAYGGFSISMTPFFSATRIVLGRHLGTVFCFANIRGGGEYGE-EWHKSGALANKQN 613

Query: 647 SIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGAAINMRPDLFRAAILKVPFLDICN 706
              DFIS A++LV   Y    +L   G S GG+LVGA IN RPDLF  A+  V  +D+  
Sbjct: 614 CFDDFISGAEYLVSAGYTQPRKLCIEGGSNGGILVGACINQRPDLFGCALAHVGVMDM-- 673

Query: 707 TLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDNISRG--------SCYPPMLVTSS 766
            L      +      EFG     ++F  ++ YSP  N+ R           YP  ++ ++
Sbjct: 674 -LRFHKFTIGHAWTSEFGCSDKEEEFHWLIKYSPLHNVKRPWEQKTDLFFQYPSTMLLTA 728

Query: 767 FRDARVGVWEAAKWVAKIR-----DTTCSRCSTSAILKTNMLGGHFGEGGLYGGCEETAY 783
             D RV    + K +A ++         S  +   I +  +  GH          +E A 
Sbjct: 734 DHDDRVVPLHSYKLLATMQYELGLSLENSPQTNPIIARIEVKAGHGAGRPTQKMIDEAAD 728

BLAST of MC09g1598.1 vs. TAIR 10
Match: AT1G76140.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 117.5 bits (293), Expect = 4.9e-26
Identity = 83/275 (30.18%), Postives = 131/275 (47.64%), Query Frame = 0

Query: 522 SHDGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPYRLSLLDR-GFVLAFAD 581
           S DG +IP+ I+        G  P +L+ YG +   +  S+   R+ L    G V  FA+
Sbjct: 522 SKDGTKIPMFIVAKKDIKLDGSHPCLLYAYGGFNISITPSFSASRIVLSKHLGVVFCFAN 581

Query: 582 VRGGGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQLGSIGYSAGGLLVGA 641
           +RGGG  G+  WH++GS  +KQN   DFIS A++LV   Y   ++L   G S GGLLVGA
Sbjct: 582 IRGGGEYGE-EWHKAGSLAKKQNCFDDFISGAEYLVSAGYTQPSKLCIEGGSNGGLLVGA 641

Query: 642 AINMRPDLFRAAILKVPFLDICNTLLDPSLPLTILDYEEFGNPQLPKQFESILNYSPYDN 701
            IN RPDL+  A+  V  +D+   L      +      ++G  +  ++F  ++ YSP  N
Sbjct: 642 CINQRPDLYGCALAHVGVMDM---LRFHKFTIGHAWTSDYGCSENEEEFHWLIKYSPLHN 701

Query: 702 ISRG--------SCYPPMLVTSSFRDARVGVWEAAKWVAKIRDTTCSRCSTS-----AIL 761
           + R           YP  ++ ++  D RV    + K +A ++   C+    S      I 
Sbjct: 702 VKRPWEQQTDHLVQYPSTMLLTADHDDRVVPLHSLKLLATLQHVLCTSLDNSPQMNPIIG 761

Query: 762 KTNMLGGHFGEGGLYGGCEETAYEYAFLIKVLGTS 783
           +  +  GH          +E A  Y+F+ K++  S
Sbjct: 762 RIEVKAGHGAGRPTQKMIDEAADRYSFMAKMVNAS 792