MC03g0289.1 (mRNA) Bitter gourd (Dali-11) v1

BLAST of MC03g0289.1 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 5.5e-14
Identity = 48/111 (43.24%), Postives = 65/111 (58.56%), Query Frame = 0

Query: 10  LVVLLPAMVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYNRKYPSPTPL 69
           +VVLLP   +AAA          + G + PI N++DP + EIG +A  EYN++  S + L
Sbjct: 14  VVVLLPLYASAAA----------RVGGWSPISNVTDPQVVEIGEFAVSEYNKR--SESGL 73

Query: 70  VFQKVVSGEKQVVNGYNYKLIMYIKSG-GLIPQYEVIVYDIPWKSHRELTS 120
            F+ VVSGE QVV+G NY+L +    G G+   Y  IV+D PW   R LTS
Sbjct: 74  KFETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTS 112

BLAST of MC03g0289.1 vs. ExPASy Swiss-Prot
Match: Q10Q46 (Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210200 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 3.9e-12
Identity = 45/109 (41.28%), Postives = 61/109 (55.96%), Query Frame = 0

Query: 13  LLPAMVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYNRKYPSPTPLVFQ 72
           LL A V AAA  P           + PIKN+ DP+I E+GR+A  E NR  PS   L F 
Sbjct: 8   LLLAAVCAAAALPR---------GWSPIKNIDDPHIQELGRWAITENNRVSPS-DELTFH 67

Query: 73  KVVSGEKQVVNGYNYKLIMYIKSGG--LIPQYEVIVYDIPWKSHRELTS 120
           +V  GE+QVV+G NY+L +   SGG  +   Y  +V++  W + R+L S
Sbjct: 68  RVTGGEQQVVSGMNYRLEIEAASGGGDVTGSYGAVVFEQEWSNTRKLIS 106

BLAST of MC03g0289.1 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 68.2 bits (165), Expect = 7.4e-11
Identity = 39/113 (34.51%), Postives = 64/113 (56.64%), Query Frame = 0

Query: 7   PLFLVVLLPAMVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYNRKYPSP 66
           PL L++LL   ++AA        +    G + PI+NL+   + ++ ++A  E+N++  + 
Sbjct: 5   PLSLLLLLLLALSAAVVGGR---KRVAAGGWRPIENLNSAEVQDVAQFAVSEHNKQ--AN 64

Query: 67  TPLVFQKVVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTS 120
             L +Q VV G  QVV G NY+L++  K G ++  YE +V+D PW   R LTS
Sbjct: 65  DELQYQSVVRGYTQVVAGTNYRLVIAAKDGAVVGNYEAVVWDKPWMHFRNLTS 112

BLAST of MC03g0289.1 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 4.8e-10
Identity = 37/113 (32.74%), Postives = 63/113 (55.75%), Query Frame = 0

Query: 7   PLFLVVLLPAMVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYNRKYPSP 66
           PL L++ L   ++AA      +      G + PI++L+   + ++ ++A  E+N++  + 
Sbjct: 5   PLSLLLFLLLALSAAVVGGRKLV---AAGGWRPIESLNSAEVQDVAQFAVSEHNKQ--AN 64

Query: 67  TPLVFQKVVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTS 120
             L +Q VV G  QVV G NY+L++  K G ++  YE +V+D PW   R LTS
Sbjct: 65  DELQYQSVVRGYTQVVAGTNYRLVIAAKDGAVVGNYEAVVWDKPWMHFRNLTS 112

BLAST of MC03g0289.1 vs. ExPASy Swiss-Prot
Match: Q10J94 (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.8e-09
Identity = 44/115 (38.26%), Postives = 66/115 (57.39%), Query Frame = 0

Query: 7   PLFLVVLLPAMVAAAAP-SPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYNRKYPS 66
           PL L +LL    AAAA    +  + P  GG + PI ++ DP+I E+G +A +E +    S
Sbjct: 5   PLLLALLLAVSAAAAAQVGGNRGHGPLVGG-WSPITDVGDPHIQELGGWA-VERHASL-S 64

Query: 67  PTPLVFQKVVSGEKQVVNGYNYKLIMYIKS-GGLIPQYEVIVYDIPWKSHRELTS 120
              L F++V SGE+QVV+G NY+L++      G    Y  +VY+  W + R+LTS
Sbjct: 65  SDGLRFRRVTSGEQQVVSGMNYRLVVSASDPAGATASYVAVVYEQSWTNTRQLTS 116

BLAST of MC03g0289.1 vs. NCBI nr
Match: XP_022156944.1 (cysteine proteinase inhibitor 5-like [Momordica charantia])

HSP 1 Score: 241 bits (616), Expect = 2.47e-80
Identity = 120/120 (100.00%), Postives = 120/120 (100.00%), Query Frame = 0

Query: 1   MKFFSAPLFLVVLLPAMVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYN 60
           MKFFSAPLFLVVLLPAMVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYN
Sbjct: 1   MKFFSAPLFLVVLLPAMVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYN 60

Query: 61  RKYPSPTPLVFQKVVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTSL 120
           RKYPSPTPLVFQKVVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTSL
Sbjct: 61  RKYPSPTPLVFQKVVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTSL 120

BLAST of MC03g0289.1 vs. NCBI nr
Match: XP_022147176.1 (cysteine proteinase inhibitor 1-like [Momordica charantia])

HSP 1 Score: 143 bits (360), Expect = 2.27e-40
Identity = 69/86 (80.23%), Postives = 75/86 (87.21%), Query Frame = 0

Query: 35  GQYEPIKNLSDPYISEIGRYACIEYNRKYPSPTPLVFQKVVSGEKQVVNGYNYKLIMYIK 94
           G Y+PIKNLSDPYISEIGRYACIEYNRK P+PTPLVF+KVVSGE+QVV G NYKLI+Y K
Sbjct: 108 GGYQPIKNLSDPYISEIGRYACIEYNRKNPNPTPLVFKKVVSGEEQVVAGTNYKLILYTK 167

Query: 95  SGGLIPQYEVIVYDIPWKSHRELTSL 120
            G L   YEVIVYD PW+SHRELTSL
Sbjct: 168 RGSLNLNYEVIVYDKPWESHRELTSL 193

BLAST of MC03g0289.1 vs. NCBI nr
Match: XP_022156941.1 (cysteine proteinase inhibitor 5-like [Momordica charantia])

HSP 1 Score: 105 bits (263), Expect = 1.24e-26
Identity = 60/114 (52.63%), Postives = 73/114 (64.04%), Query Frame = 0

Query: 17  MVAAAAPSPSPVYQPSQG--------GQYEPIKNLSDPYISEIGRYACIEYNRKYPSP-- 76
           MVAAAA +PSP   P           G YE IK+++DP+I EIGRYACIEYNR + +   
Sbjct: 1   MVAAAALTPSPTSTPVAAAEPHSHKLGDYETIKDVNDPFIHEIGRYACIEYNRIHRNDSK 60

Query: 77  -TPLVFQKVVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTS 119
            TP V++ VV G++ VV G NYKL++ I        YEVIVYD PW SHRELTS
Sbjct: 61  FTPHVYENVVEGKEAVVAGTNYKLVLKITINKQSHNYEVIVYDRPWDSHRELTS 114

BLAST of MC03g0289.1 vs. NCBI nr
Match: XP_022156942.1 (cysteine proteinase inhibitor 1-like [Momordica charantia])

HSP 1 Score: 102 bits (253), Expect = 2.97e-25
Identity = 57/106 (53.77%), Postives = 69/106 (65.09%), Query Frame = 0

Query: 17  MVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYNR---KYPSPTPLVFQK 76
           MVAAAA    P +     G YEPIK+++  +I EIGRYACIEYNR   K P  TP V+QK
Sbjct: 1   MVAAAAAVEPPSHAK---GAYEPIKDVNASFIQEIGRYACIEYNRINEKKPEFTPHVYQK 60

Query: 77  VVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTS 119
           VV GE+QVV G NY+L + +        Y+ IVYD  W+SHRELTS
Sbjct: 61  VVKGEQQVVGGMNYRLALMVTVNKQSQYYQAIVYDRSWESHRELTS 103

BLAST of MC03g0289.1 vs. NCBI nr
Match: XP_041025983.1 (LOW QUALITY PROTEIN: cysteine proteinase inhibitor 5 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 87.8 bits (216), Expect = 1.41e-19
Identity = 47/106 (44.34%), Postives = 66/106 (62.26%), Query Frame = 0

Query: 14  LPAMVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYNRKYPSPTPLVFQK 73
           LP   AAAA  P         G ++PIKN+ DP++ EIG +A  EYN+   S   L FQ 
Sbjct: 14  LPIFSAAAARGPLL-------GGWQPIKNVEDPHVKEIGEFAVSEYNKT--SKASLKFQN 73

Query: 74  VVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTS 119
           VV GE QVV+G NY+L++  K GG+   Y+ IV++ PW++ ++LTS
Sbjct: 74  VVKGETQVVSGTNYRLVVVAKDGGVTKNYQAIVWEKPWENFKKLTS 110

BLAST of MC03g0289.1 vs. ExPASy TrEMBL
Match: A0A6J1DV34 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111023777 PE=4 SV=1)

HSP 1 Score: 241 bits (616), Expect = 1.20e-80
Identity = 120/120 (100.00%), Postives = 120/120 (100.00%), Query Frame = 0

Query: 1   MKFFSAPLFLVVLLPAMVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYN 60
           MKFFSAPLFLVVLLPAMVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYN
Sbjct: 1   MKFFSAPLFLVVLLPAMVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYN 60

Query: 61  RKYPSPTPLVFQKVVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTSL 120
           RKYPSPTPLVFQKVVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTSL
Sbjct: 61  RKYPSPTPLVFQKVVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTSL 120

BLAST of MC03g0289.1 vs. ExPASy TrEMBL
Match: A0A6J1D1J8 (cysteine proteinase inhibitor 1-like OS=Momordica charantia OX=3673 GN=LOC111016188 PE=4 SV=1)

HSP 1 Score: 143 bits (360), Expect = 1.10e-40
Identity = 69/86 (80.23%), Postives = 75/86 (87.21%), Query Frame = 0

Query: 35  GQYEPIKNLSDPYISEIGRYACIEYNRKYPSPTPLVFQKVVSGEKQVVNGYNYKLIMYIK 94
           G Y+PIKNLSDPYISEIGRYACIEYNRK P+PTPLVF+KVVSGE+QVV G NYKLI+Y K
Sbjct: 108 GGYQPIKNLSDPYISEIGRYACIEYNRKNPNPTPLVFKKVVSGEEQVVAGTNYKLILYTK 167

Query: 95  SGGLIPQYEVIVYDIPWKSHRELTSL 120
            G L   YEVIVYD PW+SHRELTSL
Sbjct: 168 RGSLNLNYEVIVYDKPWESHRELTSL 193

BLAST of MC03g0289.1 vs. ExPASy TrEMBL
Match: A0A6J1DWG9 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111023773 PE=4 SV=1)

HSP 1 Score: 105 bits (263), Expect = 5.99e-27
Identity = 60/114 (52.63%), Postives = 73/114 (64.04%), Query Frame = 0

Query: 17  MVAAAAPSPSPVYQPSQG--------GQYEPIKNLSDPYISEIGRYACIEYNRKYPSP-- 76
           MVAAAA +PSP   P           G YE IK+++DP+I EIGRYACIEYNR + +   
Sbjct: 1   MVAAAALTPSPTSTPVAAAEPHSHKLGDYETIKDVNDPFIHEIGRYACIEYNRIHRNDSK 60

Query: 77  -TPLVFQKVVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTS 119
            TP V++ VV G++ VV G NYKL++ I        YEVIVYD PW SHRELTS
Sbjct: 61  FTPHVYENVVEGKEAVVAGTNYKLVLKITINKQSHNYEVIVYDRPWDSHRELTS 114

BLAST of MC03g0289.1 vs. ExPASy TrEMBL
Match: A0A6J1DTA4 (cysteine proteinase inhibitor 1-like OS=Momordica charantia OX=3673 GN=LOC111023774 PE=4 SV=1)

HSP 1 Score: 102 bits (253), Expect = 1.44e-25
Identity = 57/106 (53.77%), Postives = 69/106 (65.09%), Query Frame = 0

Query: 17  MVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYNR---KYPSPTPLVFQK 76
           MVAAAA    P +     G YEPIK+++  +I EIGRYACIEYNR   K P  TP V+QK
Sbjct: 1   MVAAAAAVEPPSHAK---GAYEPIKDVNASFIQEIGRYACIEYNRINEKKPEFTPHVYQK 60

Query: 77  VVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTS 119
           VV GE+QVV G NY+L + +        Y+ IVYD  W+SHRELTS
Sbjct: 61  VVKGEQQVVGGMNYRLALMVTVNKQSQYYQAIVYDRSWESHRELTS 103

BLAST of MC03g0289.1 vs. ExPASy TrEMBL
Match: A0A2I4G446 (cysteine proteinase inhibitor 5 OS=Juglans regia OX=51240 GN=LOC109004561 PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.41e-19
Identity = 47/106 (44.34%), Postives = 67/106 (63.21%), Query Frame = 0

Query: 14  LPAMVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYNRKYPSPTPLVFQK 73
           LP   AAA P       P  GG ++PIKN+ DP++ EIG +A  EYN+   S   L FQ 
Sbjct: 14  LPIFSAAAGPR-----GPLLGG-WQPIKNVKDPHVKEIGEFAVSEYNKT--SKASLKFQN 73

Query: 74  VVSGEKQVVNGYNYKLIMYIKSGGLIPQYEVIVYDIPWKSHRELTS 119
           VV GE QVV+G NY+L++  + GG+   Y+ IV++ PW++ ++LTS
Sbjct: 74  VVKGETQVVSGTNYRLVVVAEDGGVTKNYQAIVWEKPWENFKKLTS 111

BLAST of MC03g0289.1 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 78.6 bits (192), Expect = 3.9e-15
Identity = 48/111 (43.24%), Postives = 65/111 (58.56%), Query Frame = 0

Query: 10  LVVLLPAMVAAAAPSPSPVYQPSQGGQYEPIKNLSDPYISEIGRYACIEYNRKYPSPTPL 69
           +VVLLP   +AAA          + G + PI N++DP + EIG +A  EYN++  S + L
Sbjct: 14  VVVLLPLYASAAA----------RVGGWSPISNVTDPQVVEIGEFAVSEYNKR--SESGL 73

Query: 70  VFQKVVSGEKQVVNGYNYKLIMYIKSG-GLIPQYEVIVYDIPWKSHRELTS 120
            F+ VVSGE QVV+G NY+L +    G G+   Y  IV+D PW   R LTS
Sbjct: 74  KFETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTS 112

BLAST of MC03g0289.1 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 1.7e-10
Identity = 36/86 (41.86%), Postives = 49/86 (56.98%), Query Frame = 0

Query: 35  GQYEPIKNLSDPYISEIGRYACIEYNRKYPSPTPLVFQKVVSGEKQVVNGYNYKLIMYIK 94
           G  +PIKN+SDP +  + +YA  E+N++  S   LVF KVV G  QVV+G  Y L +  K
Sbjct: 30  GSRKPIKNVSDPDVVAVAKYAIEEHNKE--SKEKLVFVKVVEGTTQVVSGTKYDLKIAAK 89

Query: 95  -SGGLIPQYEVIVYDIPWKSHRELTS 120
             GG I  YE +V +  W   + L S
Sbjct: 90  DGGGKIKNYEAVVVEKLWLHSKSLES 113