MC02g0620.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC02g0620.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RING-type domain-containing protein
Location	MC02: 4974113 .. 4975163 (+)
Sequence length	915
RNA-Seq Expression	MC02g0620.1
Synteny	MC02g0620.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGATACGGAGCGCCATTAACAGGATTCAGTGGTAATGCTGCGTCGGAGGCTCTGTTTCCGCCGTATAATTCTGTAGCGATCGATTCGTTTCCTAAAGCGGCGGTGGCCGTCGATACCGCCGCTGTCATGAAGTCCGACAGCGGTATTACCTGTAACCTTCCGATACCGAGAAAGCGGGCAAGGGATCATTCCACCATCAACCGATTGCTCTCGACCTCTTATCCTTCGCCTGCTCATTACCAGAAAAACTGCGGATGTTTTTATTTTCTTGGCGAAGATATCTCCATCCAAATTCAACAGCAGCAATTTGATCTCGACCGATTGATATCCCAACATGTACGGTACAGAACGCTGTTTCTTTCTTCTCAGGATTATATTTCAGTTGATTAATTCTTTGAGTAGGCGTTAATTATTATTATTAGTCTGGTTGAGAAAATTGCGGTTGTTTTAATATTATTATTTTCATAATTTTTGGAGGAAACAGATGGAGAAGGTGAGGATGGAAGTGGAAGAGAAGCGAAGGAGGCAAGCGAGGAGGATTTTGGAGGCGATAGAGGTAGGGATGATGAAGATATTGAGGAACAAAGAGGAGGAGATCGAGAAGATGGGGAAACTGAATTGGGAGCTAGAAGAGCGAGTGAACTCGCTGTGTATGGAGAATCAAATATGGCGCGACGTGGCGCAGACGAACGAAGCGACGGCGAATGCGCTCCGGTGCAACCTGGAGCAAGTCCTGTCGCAGGCGAAAAACGAGGCGACCGGATTGGAGTGGCCGGCGAGAACCGCAGCGGCGGCGGAGGAGGCAGAGTCGTGCTGCGGAAGCAACGGCGGCGAGGAAGAAGCGGAATCGTCGGAGAAGAGGATGAAGAGAGGGCGGAAGGGGATTTCGGAGGAGGACAGACGGTGCAAGAACTGTTGGAAAGAGGAGTCGTGCGTGTTGCTGTTGCCATGCAGGCACCTGTGTCTGTGCACTGTTTGTGGGTCATCTCTCCACACTTGTCCAATCTGCAACTCCACCAATAACGCCAGTGTTCGTGTAATCATGCCT

mRNA sequence

ATGGGATACGGAGCGCCATTAACAGGATTCAGTGGTAATGCTGCGTCGGAGGCTCTGTTTCCGCCGTATAATTCTGTAGCGATCGATTCGTTTCCTAAAGCGGCGGTGGCCGTCGATACCGCCGCTGTCATGAAGTCCGACAGCGGTATTACCTGTAACCTTCCGATACCGAGAAAGCGGGCAAGGGATCATTCCACCATCAACCGATTGCTCTCGACCTCTTATCCTTCGCCTGCTCATTACCAGAAAAACTGCGGATGTTTTTATTTTCTTGGCGAAGATATCTCCATCCAAATTCAACAGCAGCAATTTGATCTCGACCGATTGATATCCCAACATGTACGGAAACAGATGGAGAAGGTGAGGATGGAAGTGGAAGAGAAGCGAAGGAGGCAAGCGAGGAGGATTTTGGAGGCGATAGAGGTAGGGATGATGAAGATATTGAGGAACAAAGAGGAGGAGATCGAGAAGATGGGGAAACTGAATTGGGAGCTAGAAGAGCGAGTGAACTCGCTGTGTATGGAGAATCAAATATGGCGCGACGTGGCGCAGACGAACGAAGCGACGGCGAATGCGCTCCGGTGCAACCTGGAGCAAGTCCTGTCGCAGGCGAAAAACGAGGCGACCGGATTGGAGTGGCCGGCGAGAACCGCAGCGGCGGCGGAGGAGGCAGAGTCGTGCTGCGGAAGCAACGGCGGCGAGGAAGAAGCGGAATCGTCGGAGAAGAGGATGAAGAGAGGGCGGAAGGGGATTTCGGAGGAGGACAGACGGTGCAAGAACTGTTGGAAAGAGGAGTCGTGCGTGTTGCTGTTGCCATGCAGGCACCTGTGTCTGTGCACTGTTTGTGGGTCATCTCTCCACACTTGTCCAATCTGCAACTCCACCAATAACGCCAGTGTTCGTGTAATCATGCCT

Coding sequence (CDS)

ATGGGATACGGAGCGCCATTAACAGGATTCAGTGGTAATGCTGCGTCGGAGGCTCTGTTTCCGCCGTATAATTCTGTAGCGATCGATTCGTTTCCTAAAGCGGCGGTGGCCGTCGATACCGCCGCTGTCATGAAGTCCGACAGCGGTATTACCTGTAACCTTCCGATACCGAGAAAGCGGGCAAGGGATCATTCCACCATCAACCGATTGCTCTCGACCTCTTATCCTTCGCCTGCTCATTACCAGAAAAACTGCGGATGTTTTTATTTTCTTGGCGAAGATATCTCCATCCAAATTCAACAGCAGCAATTTGATCTCGACCGATTGATATCCCAACATGTACGGAAACAGATGGAGAAGGTGAGGATGGAAGTGGAAGAGAAGCGAAGGAGGCAAGCGAGGAGGATTTTGGAGGCGATAGAGGTAGGGATGATGAAGATATTGAGGAACAAAGAGGAGGAGATCGAGAAGATGGGGAAACTGAATTGGGAGCTAGAAGAGCGAGTGAACTCGCTGTGTATGGAGAATCAAATATGGCGCGACGTGGCGCAGACGAACGAAGCGACGGCGAATGCGCTCCGGTGCAACCTGGAGCAAGTCCTGTCGCAGGCGAAAAACGAGGCGACCGGATTGGAGTGGCCGGCGAGAACCGCAGCGGCGGCGGAGGAGGCAGAGTCGTGCTGCGGAAGCAACGGCGGCGAGGAAGAAGCGGAATCGTCGGAGAAGAGGATGAAGAGAGGGCGGAAGGGGATTTCGGAGGAGGACAGACGGTGCAAGAACTGTTGGAAAGAGGAGTCGTGCGTGTTGCTGTTGCCATGCAGGCACCTGTGTCTGTGCACTGTTTGTGGGTCATCTCTCCACACTTGTCCAATCTGCAACTCCACCAATAACGCCAGTGTTCGTGTAATCATGCCT

Protein sequence

MGYGAPLTGFSGNAASEALFPPYNSVAIDSFPKAAVAVDTAAVMKSDSGITCNLPIPRKRARDHSTINRLLSTSYPSPAHYQKNCGCFYFLGEDISIQIQQQQFDLDRLISQHVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWELEERVNSLCMENQIWRDVAQTNEATANALRCNLEQVLSQAKNEATGLEWPARTAAAAEEAESCCGSNGGEEEAESSEKRMKRGRKGISEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP

Homology

[Close]

BLAST of MC02g0620.1 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 3.9e-56
Identity = 145/266 (54.51%), Postives = 180/266 (67.67%), Query Frame = 0


Query: 55  PIP-RKRARDHSTIN--RLL-----STSYPSPAHYQKNCGCFYFLGEDISI--QIQQQQF 114
           P+P RKR+RD S  N   LL     S+S  + A        F FLG+DI I   + QQQ 
Sbjct: 95  PLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQH 154

Query: 115 DLDRLISQHVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWE 174
           ++DR +S H+  QME+V+ E+EEKR+RQAR I+EAIE G++K LR KEEE E++GK+N  
Sbjct: 155 EIDRFVSLHL-YQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHA 214

Query: 175 LEERVNSLCMENQIWRDVAQTNEATANALRCNLEQVLSQAK--NEATGLEWPARTAAAAE 234
           LEERV SL +ENQIWRD+AQTNEATAN LR NLE VL+Q K  +   GLE   +     +
Sbjct: 215 LEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLE---KNMNEED 274

Query: 235 EAESCCGSN--GGEEEAESSEKRMKRGRKGISEEDRR--CKNCWKEESCVLLLPCRHLCL 294
           +AESCCGS+  GG EE       ++R  +  +E  RR  C+NC +EESCVLLLPCRHLCL
Sbjct: 275 DAESCCGSSCGGGGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCL 334

Query: 295 CTVCGSSLHTCPICNSTNNASVRVIM 305
           C VCGSS+HTCPIC S  NASV V M
Sbjct: 335 CGVCGSSVHTCPICTSPKNASVHVNM 356

[Close]

BLAST of MC02g0620.1 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 4.7e-54
Identity = 144/318 (45.28%), Postives = 195/318 (61.32%), Query Frame = 0


Query: 1   MGYGAPLTGFSGNAASEALFPP-YNSVAID-----SFPKAAVAVDTAAVMKSDSGITCNL 60
           M YG   T         +LF P YN   +D     S      +VD++    SD+ +    
Sbjct: 33  MRYGTVPTFNPTVECQTSLFNPIYNISPVDRLVHQSMKPTIQSVDSSLTFNSDNNVDFLR 92

Query: 61  PI-PRKRARDHSTINRLLSTSYPSPAHYQKN-CGCFYFLGEDISIQIQQQQFDLDRLISQ 120
           P+  RKR+R+ S +  L  ++Y      QKN      FLG+D+S  +QQ  FD+DRLIS 
Sbjct: 93  PVSSRKRSREESVV--LNPSAY---MQIQKNPTDPLMFLGQDLSSNVQQHHFDIDRLISN 152

Query: 121 HVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWELEERVNSL 180
           HV    E++RME+EEKR+ Q RRI+EA+E G+MK LR K++EI  +GKLN  LEE+V SL
Sbjct: 153 HV----ERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSL 212

Query: 181 CMENQIWRDVAQTNEATANALRCNLEQVLSQAKNEATGLEWPARTAAAAEEAESCCGSNG 240
           C+ENQIWRDVAQ+NEAT NALR NL+QVL+  +       W       A++A+SCCGSN 
Sbjct: 213 CVENQIWRDVAQSNEATVNALRSNLQQVLAAVERN----RW-EEPPTVADDAQSCCGSN- 272

Query: 241 GEEEAESSEKRMKRGRKGISEEDRR--------CKNCWKEESCVLLLPCRHLCLCTVCGS 300
             +E +S E+R K    G +++ ++        C++C K E+ VLLLPCRH+CLC+VCGS
Sbjct: 273 --DEGDSEEERWKLA--GEAQDTKKMCRVGMSMCRSCGKGEASVLLLPCRHMCLCSVCGS 331

Query: 301 SLHTCPICNSTNNASVRV 303
           SL+TCPIC S   AS+ V
Sbjct: 333 SLNTCPICKSPKTASLHV 331

[Close]

BLAST of MC02g0620.1 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 6.2e-38
Identity = 107/266 (40.23%), Postives = 155/266 (58.27%), Query Frame = 0


Query: 45  KSDSGITCNLPIP---RKRARDHSTINRLLSTSYPSPAHYQKNCGCFYFLGEDISIQIQ- 104
           K++SG++ N  +P    KR RD    +        S A    +      +  ++  QIQ 
Sbjct: 61  KAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPS---LINVELVSQIQN 120

Query: 105 QQQFDLDRLISQHVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGK 164
           QQQ ++DR ++Q    Q EK+R+E+E +++ Q R +  A++  + K L+ K++EI ++  
Sbjct: 121 QQQSEIDRFVAQ----QTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRN 180

Query: 165 LNWELEERVNSLCMENQIWRDVAQTNEATANALRCNLEQVLSQAKNEATGLEWPARTAAA 224
           LNW L+ERV SL +ENQIWRD+AQTNEA AN LR NL+QVL+Q +       +P  +A  
Sbjct: 181 LNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLET------FPTASAVV 240

Query: 225 AEEAESCCGSNGGEEEAESSEKRMKRGRKGISEEDRRCKNCWKEESCVLLLPCRHLCLCT 284
            ++AES CGS  G+            G + ++     CK C + E+ VL+LPCRHLCLCT
Sbjct: 241 EDDAESSCGSCCGD-----------GGGEAVTAVGGGCKRCGEREASVLVLPCRHLCLCT 300

Query: 285 VCGSS--LHTCPICNSTNNASVRVIM 305
           VCG S  L TCP+C+   NASV V M
Sbjct: 301 VCGGSALLRTCPVCDMVMNASVHVNM 302

[Close]

BLAST of MC02g0620.1 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.3e-35
Identity = 100/210 (47.62%), Postives = 134/210 (63.81%), Query Frame = 0


Query: 98  QIQQQQFDLDRLISQHVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEK 157
           QIQQQ  ++DR ++Q    Q E +R+E+E ++R Q R +  A++  ++K L+ K+EEI +
Sbjct: 105 QIQQQNSEIDRFVAQ----QTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIR 164

Query: 158 MGKLNWELEERVNSLCMENQIWRDVAQTNEATANALRCNLEQVLSQAKNEATGLEWPART 217
           MGKLNW L+ERV +L +ENQIWRD+AQTNEATAN LR NLEQVL+Q  ++      P   
Sbjct: 165 MGKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQV-DDLDAFRRP--L 224

Query: 218 AAAAEEAESCCGS-NGGEEEAESSEKRMKRGRKGISEEDRRCKNCWKEESCVLLLPCRHL 277
              A++AES CGS +GG+  A                 +  CK C +  + VL+LPCRHL
Sbjct: 225 VEEADDAESSCGSCDGGDVTA---------------VVNGGCKRCGELTASVLVLPCRHL 284

Query: 278 CLCTVCGSS--LHTCPICNSTNNASVRVIM 305
           CLCTVCGSS  L TCP+C+    ASV V M
Sbjct: 285 CLCTVCGSSALLRTCPVCDMVMTASVHVNM 292

[Close]

BLAST of MC02g0620.1 vs. NCBI nr
Match: XP_023532101.1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 432 bits (1110), Expect = 2.30e-149
Identity = 248/330 (75.15%), Postives = 267/330 (80.91%), Query Frame = 0


Query: 1   MGYGAPLTGFSGNAASE--ALFPPYNSVAIDSFPKAAVAVDTAAVMKSDSGITCNLPIPR 60
           MGYGAPLTGFS NAA+    L PPYN +AIDSFPKAA A  TAA +KSDSGIT NLPIPR
Sbjct: 1   MGYGAPLTGFSANAAAAETTLLPPYNYIAIDSFPKAAAA-STAAGLKSDSGITYNLPIPR 60

Query: 61  KRARDHSTINRLLSTSYPSPAHYQKNCGCFY-FLGEDISIQIQQQQFDLDRLISQHVRKQ 120
           KRARDHSTINR +S  +PSPAH+QKNCGC + FLGEDIS Q QQQQFDLDRLISQH   Q
Sbjct: 61  KRARDHSTINRFVS--HPSPAHFQKNCGCNHDFLGEDISNQFQQQQFDLDRLISQH---Q 120

Query: 121 MEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWELEERVNSLCMENQ 180
           MEKVRME+EEKR+RQARRILEAIE+GMM+ILR+KEEEIEK+GKLNWELEERVNSLCME Q
Sbjct: 121 MEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCMETQ 180

Query: 181 IWRDVAQTNEATANALRCNLEQVLSQAKN---EATGLEWPARTAA-AAEEAESCCGSNGG 240
           IWRDVAQTNEATANALR NLEQVLSQ KN   +  G E P  TAA AA++AESCCGSN  
Sbjct: 181 IWRDVAQTNEATANALRSNLEQVLSQVKNNDHQGAGFEDPTTTAAVAADDAESCCGSNYD 240

Query: 241 E-----------------EEAESSEKRMKRGRKGIS-EEDRRCKNCWKEESCVLLLPCRH 300
           +                 EE E SE+R +RGRK     EDR CKNCWKEESCVLLLPCRH
Sbjct: 241 DDDDDRKQANHGRWRNLNEEVEESEERNRRGRKARKGNEDRWCKNCWKEESCVLLLPCRH 300

Query: 301 LCLCTVCGSSLHTCPICNSTNNASVRVIMP 305
           LCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 LCLCTVCGSSLHTCPICNSTNNASVRVIMP 324

[Close]

BLAST of MC02g0620.1 vs. NCBI nr
Match: XP_023532102.1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 430 bits (1105), Expect = 1.28e-148
Identity = 247/330 (74.85%), Postives = 266/330 (80.61%), Query Frame = 0


Query: 1   MGYGAPLTGFSGNAASE--ALFPPYNSVAIDSFPKAAVAVDTAAVMKSDSGITCNLPIPR 60
           MGYGAPLTGFS NAA+    L PPYN +AIDSFPKAA A  TAA +KSDSGIT NLPIPR
Sbjct: 1   MGYGAPLTGFSANAAAAETTLLPPYNYIAIDSFPKAAAA-STAAGLKSDSGITYNLPIPR 60

Query: 61  KRARDHSTINRLLSTSYPSPAHYQKNCGCFY-FLGEDISIQIQQQQFDLDRLISQHVRKQ 120
           KRARDHSTINR +S  +PSPAH+QKNCGC + FLGEDIS Q QQQQFDLDRLISQH    
Sbjct: 61  KRARDHSTINRFVS--HPSPAHFQKNCGCNHDFLGEDISNQFQQQQFDLDRLISQH---- 120

Query: 121 MEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWELEERVNSLCMENQ 180
           MEKVRME+EEKR+RQARRILEAIE+GMM+ILR+KEEEIEK+GKLNWELEERVNSLCME Q
Sbjct: 121 MEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCMETQ 180

Query: 181 IWRDVAQTNEATANALRCNLEQVLSQAKN---EATGLEWPARTAA-AAEEAESCCGSNGG 240
           IWRDVAQTNEATANALR NLEQVLSQ KN   +  G E P  TAA AA++AESCCGSN  
Sbjct: 181 IWRDVAQTNEATANALRSNLEQVLSQVKNNDHQGAGFEDPTTTAAVAADDAESCCGSNYD 240

Query: 241 E-----------------EEAESSEKRMKRGRKGIS-EEDRRCKNCWKEESCVLLLPCRH 300
           +                 EE E SE+R +RGRK     EDR CKNCWKEESCVLLLPCRH
Sbjct: 241 DDDDDRKQANHGRWRNLNEEVEESEERNRRGRKARKGNEDRWCKNCWKEESCVLLLPCRH 300

Query: 301 LCLCTVCGSSLHTCPICNSTNNASVRVIMP 305
           LCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 LCLCTVCGSSLHTCPICNSTNNASVRVIMP 323

[Close]

BLAST of MC02g0620.1 vs. NCBI nr
Match: KAG6604725.1 (putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 424 bits (1090), Expect = 3.24e-146
Identity = 245/337 (72.70%), Postives = 264/337 (78.34%), Query Frame = 0


Query: 1   MGYGAPLTGFSGNAASE--ALFPPYNSVAIDSFPKAAVAV-----DTAAVMKSDSGITCN 60
           MGYGAPLTGFS NAA+    L PPYN +AIDSFPKAA A+      TAA +KSDSGIT N
Sbjct: 1   MGYGAPLTGFSANAAAAETTLLPPYNYIAIDSFPKAAAAMTTATASTAAGLKSDSGITYN 60

Query: 61  LPIPRKRARDHSTINRLLSTSYPSPAHYQKNCGCFY-FLGEDISIQIQQQQFDLDRLISQ 120
           LPIPRKRARDHSTINR    SYPSPAH+QKNCGC + FLGEDIS Q  QQQFDLDRLISQ
Sbjct: 61  LPIPRKRARDHSTINRF--ASYPSPAHFQKNCGCNHDFLGEDISNQFHQQQFDLDRLISQ 120

Query: 121 HVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWELEERVNSL 180
           H    MEKVRME+EEKR+RQARRILEAIE+GMM+ILR+KEEEIEK+GKLNWELEERVNSL
Sbjct: 121 H----MEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSL 180

Query: 181 CMENQIWRDVAQTNEATANALRCNLEQVLSQAKN---EATGLEWPARTAAAA-EEAESCC 240
           CME QIWRDVAQTNEATANALR NLEQVLSQ +N   +  G E P  TAA A ++AESCC
Sbjct: 181 CMETQIWRDVAQTNEATANALRSNLEQVLSQVRNNDHQGAGFEDPTTTAAVAVDDAESCC 240

Query: 241 GSNGGE-------------------EEAESSEKRMKRGRKGIS-EEDRRCKNCWKEESCV 300
           GSN  +                   EE E SE+R +RGRK     EDR CKNCWKEESCV
Sbjct: 241 GSNYDDDDDDDDRKQANPGRWRNLNEEVEESEERNRRGRKTRKGNEDRWCKNCWKEESCV 300

Query: 301 LLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 305
           LLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 LLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 331

[Close]

BLAST of MC02g0620.1 vs. NCBI nr
Match: XP_022970878.1 (probable BOI-related E3 ubiquitin-protein ligase 3 [Cucurbita maxima])

HSP 1 Score: 424 bits (1089), Expect = 3.86e-146
Identity = 245/333 (73.57%), Postives = 262/333 (78.68%), Query Frame = 0


Query: 1   MGYGAPLTGFSGNAASE--ALFPPYNSVAIDSFPKAAVAVDTAAVMKSDSGITCNLPIPR 60
           MGYGAP TGFS NAA+    L PPYN +AIDSFPKAA A  TAA +KSDSGIT NLPIPR
Sbjct: 1   MGYGAPFTGFSANAAAAETTLLPPYNYIAIDSFPKAAAA-STAAGLKSDSGITYNLPIPR 60

Query: 61  KRARDHSTINRLLSTSYPSPAHYQKNCGC-FYFLGEDISIQIQQQQFDLDRLISQHVRKQ 120
           KRARDHSTINR +S  YPSPAH+QKNCGC   FLGEDIS Q Q QQFDLDRLISQH    
Sbjct: 61  KRARDHSTINRFVS--YPSPAHFQKNCGCNLDFLGEDISNQFQHQQFDLDRLISQH---- 120

Query: 121 MEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWELEERVNSLCMENQ 180
           MEKVRME+EEKR+RQARRILEAIE+GMM+ILR+KEEEIEK+GKLNWELEERVNSLCME Q
Sbjct: 121 MEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCMETQ 180

Query: 181 IWRDVAQTNEATANALRCNLEQVLSQAKN---EATGLEWPARTAA-AAEEAESCCGSNGG 240
           IWRDVAQTNEATANALR NLEQVLSQ +N   +  G E P  TAA AA++AESCCGSN  
Sbjct: 181 IWRDVAQTNEATANALRSNLEQVLSQVRNNDHQGAGFEDPTTTAAVAADDAESCCGSNYN 240

Query: 241 E--------------------EEAESSEKRMKRGRKGIS-EEDRRCKNCWKEESCVLLLP 300
           +                    EE E SE R +RGRK     EDR CKNCWKEESCVLLLP
Sbjct: 241 DDGDDDDDRKQANHGPWRNLNEEVEESEGRNRRGRKTRKGNEDRWCKNCWKEESCVLLLP 300

Query: 301 CRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 305
           CRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 CRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 326

[Close]

BLAST of MC02g0620.1 vs. NCBI nr
Match: KAG7034854.1 (putative BOI-related E3 ubiquitin-protein ligase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 424 bits (1089), Expect = 4.29e-146
Identity = 245/334 (73.35%), Postives = 264/334 (79.04%), Query Frame = 0


Query: 1   MGYGAPLTGFSGNAASE--ALFPPYNSVAIDSFPKAAVAV-----DTAAVMKSDSGITCN 60
           MGYGAPLTGFS NAA+    L PPYN +AIDSFPKAA A+      TAA +KSDSGIT N
Sbjct: 1   MGYGAPLTGFSANAAAAETTLLPPYNYIAIDSFPKAAAAMTTATASTAAGLKSDSGITYN 60

Query: 61  LPIPRKRARDHSTINRLLSTSYPSPAHYQKNCGCFY-FLGEDISIQIQQQQFDLDRLISQ 120
           L IPRKRARDHSTINR    SYPSPAH+QKNCGC + FLGEDIS Q  QQQFDLDRLISQ
Sbjct: 61  LRIPRKRARDHSTINRF--ASYPSPAHFQKNCGCNHDFLGEDISDQFHQQQFDLDRLISQ 120

Query: 121 HVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWELEERVNSL 180
           H   QMEKVRME+EEKR+RQARRILEAIE+GMM+ILR+KEEEIEK+GKLNWELEERVNSL
Sbjct: 121 H---QMEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSL 180

Query: 181 CMENQIWRDVAQTNEATANALRCNLEQVLSQAKN---EATGLEWPARTAAAA-EEAESCC 240
           CME QIWRDVAQTNEATANALR NLEQVLSQ +N   +  G E P  TAA A ++AESCC
Sbjct: 181 CMETQIWRDVAQTNEATANALRSNLEQVLSQVRNNDHQGAGFEDPTTTAAVAVDDAESCC 240

Query: 241 GSNGGE----------------EEAESSEKRMKRGRKGIS-EEDRRCKNCWKEESCVLLL 300
           GSN  +                EE E SE+R +RGRK     EDR CKNCWKEESCVLLL
Sbjct: 241 GSNYDDDDDRKQANHGRWRNLNEEVEESEERNRRGRKTRKGNEDRWCKNCWKEESCVLLL 300

Query: 301 PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 305
           PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 329

[Close]

BLAST of MC02g0620.1 vs. ExPASy TrEMBL
Match: A0A6J1I570 (probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita maxima OX=3661 GN=LOC111469724 PE=4 SV=1)

HSP 1 Score: 424 bits (1089), Expect = 1.87e-146
Identity = 245/333 (73.57%), Postives = 262/333 (78.68%), Query Frame = 0


Query: 1   MGYGAPLTGFSGNAASE--ALFPPYNSVAIDSFPKAAVAVDTAAVMKSDSGITCNLPIPR 60
           MGYGAP TGFS NAA+    L PPYN +AIDSFPKAA A  TAA +KSDSGIT NLPIPR
Sbjct: 1   MGYGAPFTGFSANAAAAETTLLPPYNYIAIDSFPKAAAA-STAAGLKSDSGITYNLPIPR 60

Query: 61  KRARDHSTINRLLSTSYPSPAHYQKNCGC-FYFLGEDISIQIQQQQFDLDRLISQHVRKQ 120
           KRARDHSTINR +S  YPSPAH+QKNCGC   FLGEDIS Q Q QQFDLDRLISQH    
Sbjct: 61  KRARDHSTINRFVS--YPSPAHFQKNCGCNLDFLGEDISNQFQHQQFDLDRLISQH---- 120

Query: 121 MEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWELEERVNSLCMENQ 180
           MEKVRME+EEKR+RQARRILEAIE+GMM+ILR+KEEEIEK+GKLNWELEERVNSLCME Q
Sbjct: 121 MEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCMETQ 180

Query: 181 IWRDVAQTNEATANALRCNLEQVLSQAKN---EATGLEWPARTAA-AAEEAESCCGSNGG 240
           IWRDVAQTNEATANALR NLEQVLSQ +N   +  G E P  TAA AA++AESCCGSN  
Sbjct: 181 IWRDVAQTNEATANALRSNLEQVLSQVRNNDHQGAGFEDPTTTAAVAADDAESCCGSNYN 240

Query: 241 E--------------------EEAESSEKRMKRGRKGIS-EEDRRCKNCWKEESCVLLLP 300
           +                    EE E SE R +RGRK     EDR CKNCWKEESCVLLLP
Sbjct: 241 DDGDDDDDRKQANHGPWRNLNEEVEESEGRNRRGRKTRKGNEDRWCKNCWKEESCVLLLP 300

Query: 301 CRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 305
           CRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 CRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 326

[Close]

BLAST of MC02g0620.1 vs. ExPASy TrEMBL
Match: A0A6J1G785 (probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita moschata OX=3662 GN=LOC111451502 PE=4 SV=1)

HSP 1 Score: 422 bits (1084), Expect = 1.15e-145
Identity = 244/334 (73.05%), Postives = 263/334 (78.74%), Query Frame = 0


Query: 1   MGYGAPLTGFSGNAASE--ALFPPYNSVAIDSFPKAAVAV-----DTAAVMKSDSGITCN 60
           MGYGAPLTGFS NAA+    L PPYN +AIDSFPKAA A+      TAA +KSDSGIT N
Sbjct: 1   MGYGAPLTGFSANAAAAETTLLPPYNYIAIDSFPKAAAAMTTATASTAAGLKSDSGITYN 60

Query: 61  LPIPRKRARDHSTINRLLSTSYPSPAHYQKNCGCFY-FLGEDISIQIQQQQFDLDRLISQ 120
           L IPRKRARDHSTINR    SYPSPAH+QKNCGC + FLGEDIS Q  QQQFDLDRLISQ
Sbjct: 61  LRIPRKRARDHSTINRF--ASYPSPAHFQKNCGCNHDFLGEDISDQFHQQQFDLDRLISQ 120

Query: 121 HVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWELEERVNSL 180
           H    MEKVRME+EEKR+RQARRILEAIE+GMM+ILR+KEEEIEK+GKLNWELEERVNSL
Sbjct: 121 H----MEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSL 180

Query: 181 CMENQIWRDVAQTNEATANALRCNLEQVLSQAKN---EATGLEWPARTAAAA-EEAESCC 240
           CME QIWRDVAQTNEATANALR NLEQVLSQ +N   +  G E P  TAA A ++AESCC
Sbjct: 181 CMETQIWRDVAQTNEATANALRSNLEQVLSQVRNNDHQGAGFEDPTTTAAVAVDDAESCC 240

Query: 241 GSNGGE----------------EEAESSEKRMKRGRKGIS-EEDRRCKNCWKEESCVLLL 300
           GSN  +                EE E SE+R +RGRK     EDR CKNCWKEESCVLLL
Sbjct: 241 GSNYDDDDDRKQANHGRWRNLNEEVEESEERNRRGRKTRKGNEDRWCKNCWKEESCVLLL 300

Query: 301 PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 305
           PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 328

[Close]

BLAST of MC02g0620.1 vs. ExPASy TrEMBL
Match: A0A6J1JF86 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483871 PE=4 SV=1)

HSP 1 Score: 407 bits (1047), Expect = 2.50e-140
Identity = 237/322 (73.60%), Postives = 253/322 (78.57%), Query Frame = 0


Query: 1   MGYGAPLTGFSGN--AASEALFPPYNSVAIDSFPKA------AVAVDTAAVMKSDSGITC 60
           MGYGAPLTGFS N  AA   L PPYNSVAIDSFPKA      + +  T  VMKSDSGI+ 
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKALGAMPASASASTGVVMKSDSGISY 60

Query: 61  NLPIPRKRARDHSTINRLLSTSYPSP-AHYQKNCGC-FYFLGEDISIQIQQQQFDLDRLI 120
           NLPIPRKR RDHSTINR LS  YPS  AH QKNCG  FYFLGEDISI +QQQQ DL+RLI
Sbjct: 61  NLPIPRKRGRDHSTINRFLS--YPSSHAHSQKNCGRNFYFLGEDISILVQQQQLDLERLI 120

Query: 121 SQHVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWELEERVN 180
           SQH   QMEKVRME+EEKR+R+ARRI+EAIEVGMM ILR+KEEEIEKMGKLNWELEERVN
Sbjct: 121 SQH---QMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVN 180

Query: 181 SLCMENQIWRDVAQTNEATANALRCNLEQVLSQAKNEATGLEWPARTAAAAEEAESCCGS 240
           SLCMENQIWRDVA+TNEATANALR +LE VL   K E    E         E+AESCCGS
Sbjct: 181 SLCMENQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEE--------EEKAESCCGS 240

Query: 241 NGG---EEEAESSEKRMKRGRKGI----SEEDRRCKNCWKEESCVLLLPCRHLCLCTVCG 300
           NGG   EEE E  E+  ++GRK       EEDRRCKNCWKEESCVLLLPCRHLCLCTVCG
Sbjct: 241 NGGDMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCG 300

Query: 301 SSLHTCPICNSTNNASVRVIMP 305
           SSLH CPICNSTNNASVRVIMP
Sbjct: 301 SSLHHCPICNSTNNASVRVIMP 309

[Close]

BLAST of MC02g0620.1 vs. ExPASy TrEMBL
Match: A0A6J1JCV5 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483871 PE=4 SV=1)

HSP 1 Score: 405 bits (1042), Expect = 1.39e-139
Identity = 236/322 (73.29%), Postives = 252/322 (78.26%), Query Frame = 0


Query: 1   MGYGAPLTGFSGN--AASEALFPPYNSVAIDSFPKA------AVAVDTAAVMKSDSGITC 60
           MGYGAPLTGFS N  AA   L PPYNSVAIDSFPKA      + +  T  VMKSDSGI+ 
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKALGAMPASASASTGVVMKSDSGISY 60

Query: 61  NLPIPRKRARDHSTINRLLSTSYPSP-AHYQKNCGC-FYFLGEDISIQIQQQQFDLDRLI 120
           NLPIPRKR RDHSTINR LS  YPS  AH QKNCG  FYFLGEDISI +QQQQ DL+RLI
Sbjct: 61  NLPIPRKRGRDHSTINRFLS--YPSSHAHSQKNCGRNFYFLGEDISILVQQQQLDLERLI 120

Query: 121 SQHVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWELEERVN 180
           SQH    MEKVRME+EEKR+R+ARRI+EAIEVGMM ILR+KEEEIEKMGKLNWELEERVN
Sbjct: 121 SQH----MEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVN 180

Query: 181 SLCMENQIWRDVAQTNEATANALRCNLEQVLSQAKNEATGLEWPARTAAAAEEAESCCGS 240
           SLCMENQIWRDVA+TNEATANALR +LE VL   K E    E         E+AESCCGS
Sbjct: 181 SLCMENQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEE--------EEKAESCCGS 240

Query: 241 NGG---EEEAESSEKRMKRGRKGI----SEEDRRCKNCWKEESCVLLLPCRHLCLCTVCG 300
           NGG   EEE E  E+  ++GRK       EEDRRCKNCWKEESCVLLLPCRHLCLCTVCG
Sbjct: 241 NGGDMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCG 300

Query: 301 SSLHTCPICNSTNNASVRVIMP 305
           SSLH CPICNSTNNASVRVIMP
Sbjct: 301 SSLHHCPICNSTNNASVRVIMP 308

[Close]

BLAST of MC02g0620.1 vs. ExPASy TrEMBL
Match: A0A6J1FS66 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448331 PE=4 SV=1)

HSP 1 Score: 403 bits (1035), Expect = 1.67e-138
Identity = 236/322 (73.29%), Postives = 251/322 (77.95%), Query Frame = 0


Query: 1   MGYGAPLTGFSGN--AASEALFPPYNSVAIDSFPKAAVAV------DTAAVMKSDSGITC 60
           MGYGAPLTGFS N  AA   L PPYNSVAIDSFPKA  A+       T  VMKSDS I+ 
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60

Query: 61  NLPIPRKRARDHSTINRLLSTSYPSP-AHYQKNCGC-FYFLGEDISIQIQQQQFDLDRLI 120
           NLPIPRKR RDHSTINR LS  YPS  AH QKNCG   YFLGEDISI +QQQQ DL+RLI
Sbjct: 61  NLPIPRKRGRDHSTINRFLS--YPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLI 120

Query: 121 SQHVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWELEERVN 180
           SQH   QMEKVRME+EEKR+R+ARRI+EAIEVGMM ILR+KEEEIEKMGKLNWELEERVN
Sbjct: 121 SQH---QMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVN 180

Query: 181 SLCMENQIWRDVAQTNEATANALRCNLEQVLSQAKNEATGLEWPARTAAAAEEAESCCGS 240
           SLCMENQIWRDVA+TNEATANALR +LE VL   K E    E         E+AESCCGS
Sbjct: 181 SLCMENQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEE--------EEKAESCCGS 240

Query: 241 NGG---EEEAESSEKRMKRGRKGI----SEEDRRCKNCWKEESCVLLLPCRHLCLCTVCG 300
           NGG   EEE E  E+  ++GRK       EEDRRCKNCWKEESCVLLLPCRHLCLCTVCG
Sbjct: 241 NGGDMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCG 300

Query: 301 SSLHTCPICNSTNNASVRVIMP 305
           SSLH CPICNSTNNASVRVIMP
Sbjct: 301 SSLHHCPICNSTNNASVRVIMP 309

[Close]

BLAST of MC02g0620.1 vs. TAIR 10
Match: AT1G79110.1 (zinc ion binding )

HSP 1 Score: 219.9 bits (559), Expect = 2.7e-57
Identity = 145/266 (54.51%), Postives = 180/266 (67.67%), Query Frame = 0


Query: 55  PIP-RKRARDHSTIN--RLL-----STSYPSPAHYQKNCGCFYFLGEDISI--QIQQQQF 114
           P+P RKR+RD S  N   LL     S+S  + A        F FLG+DI I   + QQQ 
Sbjct: 95  PLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQH 154

Query: 115 DLDRLISQHVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWE 174
           ++DR +S H+  QME+V+ E+EEKR+RQAR I+EAIE G++K LR KEEE E++GK+N  
Sbjct: 155 EIDRFVSLHL-YQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHA 214

Query: 175 LEERVNSLCMENQIWRDVAQTNEATANALRCNLEQVLSQAK--NEATGLEWPARTAAAAE 234
           LEERV SL +ENQIWRD+AQTNEATAN LR NLE VL+Q K  +   GLE   +     +
Sbjct: 215 LEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLE---KNMNEED 274

Query: 235 EAESCCGSN--GGEEEAESSEKRMKRGRKGISEEDRR--CKNCWKEESCVLLLPCRHLCL 294
           +AESCCGS+  GG EE       ++R  +  +E  RR  C+NC +EESCVLLLPCRHLCL
Sbjct: 275 DAESCCGSSCGGGGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCL 334

Query: 295 CTVCGSSLHTCPICNSTNNASVRVIM 305
           C VCGSS+HTCPIC S  NASV V M
Sbjct: 335 CGVCGSSVHTCPICTSPKNASVHVNM 356

[Close]

BLAST of MC02g0620.1 vs. TAIR 10
Match: AT1G79110.2 (zinc ion binding )

HSP 1 Score: 218.0 bits (554), Expect = 1.0e-56
Identity = 144/266 (54.14%), Postives = 178/266 (66.92%), Query Frame = 0


Query: 55  PIP-RKRARDHSTIN--RLL-----STSYPSPAHYQKNCGCFYFLGEDISI--QIQQQQF 114
           P+P RKR+RD S  N   LL     S+S  + A        F FLG+DI I   + QQQ 
Sbjct: 95  PLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQH 154

Query: 115 DLDRLISQHVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWE 174
           ++DR +S H    ME+V+ E+EEKR+RQAR I+EAIE G++K LR KEEE E++GK+N  
Sbjct: 155 EIDRFVSLH----MERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHA 214

Query: 175 LEERVNSLCMENQIWRDVAQTNEATANALRCNLEQVLSQAK--NEATGLEWPARTAAAAE 234
           LEERV SL +ENQIWRD+AQTNEATAN LR NLE VL+Q K  +   GLE   +     +
Sbjct: 215 LEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLE---KNMNEED 274

Query: 235 EAESCCGSN--GGEEEAESSEKRMKRGRKGISEEDRR--CKNCWKEESCVLLLPCRHLCL 294
           +AESCCGS+  GG EE       ++R  +  +E  RR  C+NC +EESCVLLLPCRHLCL
Sbjct: 275 DAESCCGSSCGGGGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCL 334

Query: 295 CTVCGSSLHTCPICNSTNNASVRVIM 305
           C VCGSS+HTCPIC S  NASV V M
Sbjct: 335 CGVCGSSVHTCPICTSPKNASVHVNM 353

[Close]

BLAST of MC02g0620.1 vs. TAIR 10
Match: AT3G12920.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 213.0 bits (541), Expect = 3.4e-55
Identity = 144/318 (45.28%), Postives = 195/318 (61.32%), Query Frame = 0


Query: 1   MGYGAPLTGFSGNAASEALFPP-YNSVAID-----SFPKAAVAVDTAAVMKSDSGITCNL 60
           M YG   T         +LF P YN   +D     S      +VD++    SD+ +    
Sbjct: 33  MRYGTVPTFNPTVECQTSLFNPIYNISPVDRLVHQSMKPTIQSVDSSLTFNSDNNVDFLR 92

Query: 61  PI-PRKRARDHSTINRLLSTSYPSPAHYQKN-CGCFYFLGEDISIQIQQQQFDLDRLISQ 120
           P+  RKR+R+ S +  L  ++Y      QKN      FLG+D+S  +QQ  FD+DRLIS 
Sbjct: 93  PVSSRKRSREESVV--LNPSAY---MQIQKNPTDPLMFLGQDLSSNVQQHHFDIDRLISN 152

Query: 121 HVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGKLNWELEERVNSL 180
           HV    E++RME+EEKR+ Q RRI+EA+E G+MK LR K++EI  +GKLN  LEE+V SL
Sbjct: 153 HV----ERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSL 212

Query: 181 CMENQIWRDVAQTNEATANALRCNLEQVLSQAKNEATGLEWPARTAAAAEEAESCCGSNG 240
           C+ENQIWRDVAQ+NEAT NALR NL+QVL+  +       W       A++A+SCCGSN 
Sbjct: 213 CVENQIWRDVAQSNEATVNALRSNLQQVLAAVERN----RW-EEPPTVADDAQSCCGSN- 272

Query: 241 GEEEAESSEKRMKRGRKGISEEDRR--------CKNCWKEESCVLLLPCRHLCLCTVCGS 300
             +E +S E+R K    G +++ ++        C++C K E+ VLLLPCRH+CLC+VCGS
Sbjct: 273 --DEGDSEEERWKLA--GEAQDTKKMCRVGMSMCRSCGKGEASVLLLPCRHMCLCSVCGS 331

Query: 301 SLHTCPICNSTNNASVRV 303
           SL+TCPIC S   AS+ V
Sbjct: 333 SLNTCPICKSPKTASLHV 331

[Close]

BLAST of MC02g0620.1 vs. TAIR 10
Match: AT4G19700.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 159.5 bits (402), Expect = 4.4e-39
Identity = 107/266 (40.23%), Postives = 155/266 (58.27%), Query Frame = 0


Query: 45  KSDSGITCNLPIP---RKRARDHSTINRLLSTSYPSPAHYQKNCGCFYFLGEDISIQIQ- 104
           K++SG++ N  +P    KR RD    +        S A    +      +  ++  QIQ 
Sbjct: 61  KAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPS---LINVELVSQIQN 120

Query: 105 QQQFDLDRLISQHVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEKMGK 164
           QQQ ++DR ++Q    Q EK+R+E+E +++ Q R +  A++  + K L+ K++EI ++  
Sbjct: 121 QQQSEIDRFVAQ----QTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRN 180

Query: 165 LNWELEERVNSLCMENQIWRDVAQTNEATANALRCNLEQVLSQAKNEATGLEWPARTAAA 224
           LNW L+ERV SL +ENQIWRD+AQTNEA AN LR NL+QVL+Q +       +P  +A  
Sbjct: 181 LNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLET------FPTASAVV 240

Query: 225 AEEAESCCGSNGGEEEAESSEKRMKRGRKGISEEDRRCKNCWKEESCVLLLPCRHLCLCT 284
            ++AES CGS  G+            G + ++     CK C + E+ VL+LPCRHLCLCT
Sbjct: 241 EDDAESSCGSCCGD-----------GGGEAVTAVGGGCKRCGEREASVLVLPCRHLCLCT 300

Query: 285 VCGSS--LHTCPICNSTNNASVRVIM 305
           VCG S  L TCP+C+   NASV V M
Sbjct: 301 VCGGSALLRTCPVCDMVMNASVHVNM 302

[Close]

BLAST of MC02g0620.1 vs. TAIR 10
Match: AT5G45100.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 151.8 bits (382), Expect = 9.2e-37
Identity = 100/210 (47.62%), Postives = 134/210 (63.81%), Query Frame = 0


Query: 98  QIQQQQFDLDRLISQHVRKQMEKVRMEVEEKRRRQARRILEAIEVGMMKILRNKEEEIEK 157
           QIQQQ  ++DR ++Q    Q E +R+E+E ++R Q R +  A++  ++K L+ K+EEI +
Sbjct: 105 QIQQQNSEIDRFVAQ----QTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIR 164

Query: 158 MGKLNWELEERVNSLCMENQIWRDVAQTNEATANALRCNLEQVLSQAKNEATGLEWPART 217
           MGKLNW L+ERV +L +ENQIWRD+AQTNEATAN LR NLEQVL+Q  ++      P   
Sbjct: 165 MGKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQV-DDLDAFRRP--L 224

Query: 218 AAAAEEAESCCGS-NGGEEEAESSEKRMKRGRKGISEEDRRCKNCWKEESCVLLLPCRHL 277
              A++AES CGS +GG+  A                 +  CK C +  + VL+LPCRHL
Sbjct: 225 VEEADDAESSCGSCDGGDVTA---------------VVNGGCKRCGELTASVLVLPCRHL 284

Query: 278 CLCTVCGSS--LHTCPICNSTNNASVRVIM 305
           CLCTVCGSS  L TCP+C+    ASV V M
Sbjct: 285 CLCTVCGSSALLRTCPVCDMVMTASVHVNM 292

The following BLAST results are available for this feature:

BLAST of MC02g0620.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4IDI6	3.9e-56	54.51	Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37...	[more]
Q9LDD1	4.7e-54	45.28	Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37...	[more]
O81851	6.2e-38	40.23	E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1	[more]
Q9FHE4	1.3e-35	47.62	BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG...	[more]

BLAST of MC02g0620.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023532101.1	2.30e-149	75.15	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 [Cucurbita pepo su...	[more]
XP_023532102.1	1.28e-148	74.85	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita pepo su...	[more]
KAG6604725.1	3.24e-146	72.70	putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrospe...	[more]
XP_022970878.1	3.86e-146	73.57	probable BOI-related E3 ubiquitin-protein ligase 3 [Cucurbita maxima]	[more]
KAG7034854.1	4.29e-146	73.35	putative BOI-related E3 ubiquitin-protein ligase 3 [Cucurbita argyrosperma subsp...	[more]

BLAST of MC02g0620.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I570	1.87e-146	73.57	probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1G785	1.15e-145	73.05	probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1JF86	2.50e-140	73.60	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxim...	[more]
A0A6J1JCV5	1.39e-139	73.29	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxim...	[more]
A0A6J1FS66	1.67e-138	73.29	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 OS=Cucurbita mosch...	[more]

BLAST of MC02g0620.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G79110.1	2.7e-57	54.51	zinc ion binding	[more]
AT1G79110.2	1.0e-56	54.14	zinc ion binding	[more]
AT3G12920.1	3.4e-55	45.28	SBP (S-ribonuclease binding protein) family protein	[more]
AT4G19700.1	4.4e-39	40.23	SBP (S-ribonuclease binding protein) family protein	[more]
AT5G45100.1	9.2e-37	47.62	SBP (S-ribonuclease binding protein) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 148..168
None	No IPR available	COILS	Coil	Coil	coord: 111..135
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 254..294 e-value: 3.1E-8 score: 33.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 225..248
None	No IPR available	PANTHER	PTHR42647:SF12	BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 2-RELATED	coord: 5..304
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 231..305 e-value: 6.4E-13 score: 49.9
IPR017066	E3 ubiquitin-protein ligase BOI-like	PANTHER	PTHR42647	SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN	coord: 5..304
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 258..293 score: 9.081335

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC02g0620	MC02g0620	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC02g0620.1-cds	MC02g0620.1-cds-MC02:4974113..4974456	CDS
MC02g0620.1-cds	MC02g0620.1-cds-MC02:4974593..4975163	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC02g0620.1	MC02g0620.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043067	regulation of programmed cell death
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity