Lsi08G012260.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G012260.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	DNA ligase
Location	chr08: 20724514 .. 20724924 (+)
Sequence length	411
RNA-Seq Expression	Lsi08G012260.1
Synteny	Lsi08G012260.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTACCCCATTTTCATTACTCTCCTTCTTTACCTCACAAGCTTAAATCCTCCTTCTGCTGTTTCCATACCTCCCAACAACACCAGTTTCTCGACTCTCCTTCCAAGACGCCGACAAGATCGCCGTGTTCTTGGTTAAAATCTACCGCCGCTGAACTGTCGGAGTTACTTTACCGGTGCCGGTATTTCCTTGCTCGGATAGCTCGCCGCCGGAGGAAGTACCTCGCCTCCGTCGACTTTTCCTACGATTCTTCCAGTTATGCCCTCAATTTTGAGGACGACTGCCGTGACGACCATGAGTTTCCCTTCAGAGATTTCACGGCTAGATTCCCTCCGGCTATCAAGGACCCTGTTCCCCCTCCCAAATTCGCCGGATCTCAAGATGGAATCGCAATTTCCTCCTGTGGTTGA

mRNA sequence

ATGTTACCCCATTTTCATTACTCTCCTTCTTTACCTCACAAGCTTAAATCCTCCTTCTGCTGTTTCCATACCTCCCAACAACACCAGTTTCTCGACTCTCCTTCCAAGACGCCGACAAGATCGCCGTGTTCTTGGTTAAAATCTACCGCCGCTGAACTGTCGGAGTTACTTTACCGGTGCCGGTATTTCCTTGCTCGGATAGCTCGCCGCCGGAGGAAGTACCTCGCCTCCGTCGACTTTTCCTACGATTCTTCCAGTTATGCCCTCAATTTTGAGGACGACTGCCGTGACGACCATGAGTTTCCCTTCAGAGATTTCACGGCTAGATTCCCTCCGGCTATCAAGGACCCTGTTCCCCCTCCCAAATTCGCCGGATCTCAAGATGGAATCGCAATTTCCTCCTGTGGTTGA

Coding sequence (CDS)

ATGTTACCCCATTTTCATTACTCTCCTTCTTTACCTCACAAGCTTAAATCCTCCTTCTGCTGTTTCCATACCTCCCAACAACACCAGTTTCTCGACTCTCCTTCCAAGACGCCGACAAGATCGCCGTGTTCTTGGTTAAAATCTACCGCCGCTGAACTGTCGGAGTTACTTTACCGGTGCCGGTATTTCCTTGCTCGGATAGCTCGCCGCCGGAGGAAGTACCTCGCCTCCGTCGACTTTTCCTACGATTCTTCCAGTTATGCCCTCAATTTTGAGGACGACTGCCGTGACGACCATGAGTTTCCCTTCAGAGATTTCACGGCTAGATTCCCTCCGGCTATCAAGGACCCTGTTCCCCCTCCCAAATTCGCCGGATCTCAAGATGGAATCGCAATTTCCTCCTGTGGTTGA

Protein sequence

MLPHFHYSPSLPHKLKSSFCCFHTSQQHQFLDSPSKTPTRSPCSWLKSTAAELSELLYRCRYFLARIARRRRKYLASVDFSYDSSSYALNFEDDCRDDHEFPFRDFTARFPPAIKDPVPPPKFAGSQDGIAISSCG

Homology

[Close]

BLAST of Lsi08G012260.1 vs. ExPASy TrEMBL
Match: A0A6J1HE78 (uncharacterized protein LOC111462741 OS=Cucurbita moschata OX=3662 GN=LOC111462741 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.3e-51
Identity = 100/132 (75.76%), Postives = 112/132 (84.85%), Query Frame = 0


Query: 1   MLPHFHYSPSLPHKLKSSFCCFHTSQQHQFLDSPSKTPTRSPCSWLKSTAAELSELLYRC 60
           MLPHFH+SP+   KLKSSFCCFH  QQ  FLDSPSK+PTRSPCSW KST  E   +L RC
Sbjct: 1   MLPHFHHSPAFARKLKSSFCCFHGFQQDDFLDSPSKSPTRSPCSWFKSTVGEFRGILARC 60

Query: 61  RYFLARIARRRRKYLASVDFSYDSSSYALNFEDDCRDDHEFPFRDFTARFPPAIKDPVPP 120
           RY LARIARRR++YLASVDFSYDSSSYALNFEDDCR+D EFPFR+FTARFPP IK+ VP 
Sbjct: 61  RYLLARIARRRKRYLASVDFSYDSSSYALNFEDDCREDDEFPFRNFTARFPPPIKERVPA 120

Query: 121 PKFAGSQDGIAI 133
            KFAGS+DGI++
Sbjct: 121 AKFAGSEDGISV 132

[Close]

BLAST of Lsi08G012260.1 vs. ExPASy TrEMBL
Match: A0A6J1K7R1 (uncharacterized protein LOC111492424 OS=Cucurbita maxima OX=3661 GN=LOC111492424 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.9e-50
Identity = 97/132 (73.48%), Postives = 111/132 (84.09%), Query Frame = 0


Query: 1   MLPHFHYSPSLPHKLKSSFCCFHTSQQHQFLDSPSKTPTRSPCSWLKSTAAELSELLYRC 60
           MLPHFH+SP+   KLKSSFCCFH  QQ  FLDSPSK+PT+SPCSW KST  E   +L RC
Sbjct: 1   MLPHFHHSPAFARKLKSSFCCFHGFQQDDFLDSPSKSPTKSPCSWFKSTVGEFRGILARC 60

Query: 61  RYFLARIARRRRKYLASVDFSYDSSSYALNFEDDCRDDHEFPFRDFTARFPPAIKDPVPP 120
           RY LARIARRR++YLASVDF+YDSSSYALNF+DDCR+D EFPFRDFTARFPP IK+  P 
Sbjct: 61  RYLLARIARRRKRYLASVDFTYDSSSYALNFDDDCREDDEFPFRDFTARFPPPIKERAPA 120

Query: 121 PKFAGSQDGIAI 133
            KFAGS+DGI++
Sbjct: 121 AKFAGSEDGISV 132

[Close]

BLAST of Lsi08G012260.1 vs. ExPASy TrEMBL
Match: A0A1S3BDX7 (uncharacterized protein LOC103488565 OS=Cucumis melo OX=3656 GN=LOC103488565 PE=4 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 4.1e-37
Identity = 84/117 (71.79%), Postives = 89/117 (76.07%), Query Frame = 0


Query: 18  SFCCFHTSQQHQFLDSPSKTPTRSPCSWLKSTAAELSELLYRCRYFLARIARRRRKYLAS 77
           SFCCFH          PS TP  SPCSWLKSTA ELS LL RCRYFLAR+ARRR  YLAS
Sbjct: 5   SFCCFH----------PSNTPKTSPCSWLKSTALELSHLLRRCRYFLARVARRRTNYLAS 64

Query: 78  VDFSYDSSSYALNFEDDCRDDHEFPFRDFTARFPPAIKDPVP-PPKFAGSQDGIAIS 134
            DFSYDSSSYALNF+DDCRDDH+FPFRDFTARFPP +KD +P   KFAGS   I IS
Sbjct: 65  ADFSYDSSSYALNFDDDCRDDHQFPFRDFTARFPPPLKDRLPAATKFAGSHHEIPIS 111

[Close]

BLAST of Lsi08G012260.1 vs. ExPASy TrEMBL
Match: A0A6J1BQU1 (uncharacterized protein LOC111004768 OS=Momordica charantia OX=3673 GN=LOC111004768 PE=4 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 4.6e-28
Identity = 79/137 (57.66%), Postives = 92/137 (67.15%), Query Frame = 0


Query: 1   MLPHFHYSPSLPHKLKSSFCCFHTSQQHQFLDSPSKTPTRSPCSWLKSTAAELSELLYRC 60
           ML  FH+S S  HKLKSS  CF  S      D PS   T+SPCSW KSTAAE  +LL RC
Sbjct: 1   MLADFHHSSSFAHKLKSSIGCFRAS------DQPS---TKSPCSWFKSTAAEFPDLLDRC 60

Query: 61  RYFLARIAR-RRRKYLASVDFSYDSSSYALNFEDDCRDDHEFPFRDFTARFPPAIKDPVP 120
           RY +ARI R  RR+Y AS DF+YDSSSYALNFEDD R D EFP R+F AR P      +P
Sbjct: 61  RYLVARIGRGSRRRYHASPDFTYDSSSYALNFEDDSRYDDEFPLRNFAARLP-----RLP 120

Query: 121 PPKFAGSQDGIA-ISSC 136
             KFAGSQ G++ +++C
Sbjct: 121 AAKFAGSQAGVSTVAAC 123

[Close]

BLAST of Lsi08G012260.1 vs. ExPASy TrEMBL
Match: A0A7N2QYM6 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 4.8e-17
Identity = 58/116 (50.00%), Postives = 70/116 (60.34%), Query Frame = 0


Query: 10  SLPHKLKSSFCCF----HTSQQHQFLDSPSKTPTRSPCSWLKSTAAELSELLYRCRYFLA 69
           S+ HK+K+S CCF    H  QQ +  D P  T  RSP +WLKSTA EL E+ +RC  F++
Sbjct: 15  SIKHKVKTSMCCFSPSVHDHQQLKSEDKPRTTSPRSPYTWLKSTANELPEIRFRCMNFIS 74

Query: 70  RIARRRRKY---LASVDFSYDSSSYALNFEDDCRDDHEFP--FRDFTARFPPAIKD 117
           RI + RR +     S DFSYD SSYALNF+DD R D E P  F D  A  PP   D
Sbjct: 75  RIGKTRRNHHMRWHSADFSYDPSSYALNFQDDTRVDDECPLSFLDRLAASPPQPAD 130

[Close]

BLAST of Lsi08G012260.1 vs. NCBI nr
Match: XP_022962228.1 (uncharacterized protein LOC111462741 [Cucurbita moschata] >KAG6598557.1 hypothetical protein SDJN03_08335, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029495.1 hypothetical protein SDJN02_07834, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 211.5 bits (537), Expect = 4.7e-51
Identity = 100/132 (75.76%), Postives = 112/132 (84.85%), Query Frame = 0


Query: 1   MLPHFHYSPSLPHKLKSSFCCFHTSQQHQFLDSPSKTPTRSPCSWLKSTAAELSELLYRC 60
           MLPHFH+SP+   KLKSSFCCFH  QQ  FLDSPSK+PTRSPCSW KST  E   +L RC
Sbjct: 1   MLPHFHHSPAFARKLKSSFCCFHGFQQDDFLDSPSKSPTRSPCSWFKSTVGEFRGILARC 60

Query: 61  RYFLARIARRRRKYLASVDFSYDSSSYALNFEDDCRDDHEFPFRDFTARFPPAIKDPVPP 120
           RY LARIARRR++YLASVDFSYDSSSYALNFEDDCR+D EFPFR+FTARFPP IK+ VP 
Sbjct: 61  RYLLARIARRRKRYLASVDFSYDSSSYALNFEDDCREDDEFPFRNFTARFPPPIKERVPA 120

Query: 121 PKFAGSQDGIAI 133
            KFAGS+DGI++
Sbjct: 121 AKFAGSEDGISV 132

[Close]

BLAST of Lsi08G012260.1 vs. NCBI nr
Match: XP_023546798.1 (uncharacterized protein LOC111805797 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 209.1 bits (531), Expect = 2.3e-50
Identity = 99/132 (75.00%), Postives = 111/132 (84.09%), Query Frame = 0


Query: 1   MLPHFHYSPSLPHKLKSSFCCFHTSQQHQFLDSPSKTPTRSPCSWLKSTAAELSELLYRC 60
           MLPHFH+SP+   KLKSSFCCFH  QQ  FLDSPSK+PT SPCSW KST  E   +L RC
Sbjct: 1   MLPHFHHSPAFARKLKSSFCCFHGFQQDDFLDSPSKSPTTSPCSWFKSTVGEFRGILARC 60

Query: 61  RYFLARIARRRRKYLASVDFSYDSSSYALNFEDDCRDDHEFPFRDFTARFPPAIKDPVPP 120
           RY LARIARRR++YLASVDFSYDSSSYALNFEDDCR+D EFPFR+FTARFPP IK+ VP 
Sbjct: 61  RYLLARIARRRKRYLASVDFSYDSSSYALNFEDDCREDDEFPFRNFTARFPPPIKERVPA 120

Query: 121 PKFAGSQDGIAI 133
            KFAGS+DGI++
Sbjct: 121 AKFAGSEDGISV 132

[Close]

BLAST of Lsi08G012260.1 vs. NCBI nr
Match: XP_022997531.1 (uncharacterized protein LOC111492424 [Cucurbita maxima])

HSP 1 Score: 208.4 bits (529), Expect = 4.0e-50
Identity = 97/132 (73.48%), Postives = 111/132 (84.09%), Query Frame = 0


Query: 1   MLPHFHYSPSLPHKLKSSFCCFHTSQQHQFLDSPSKTPTRSPCSWLKSTAAELSELLYRC 60
           MLPHFH+SP+   KLKSSFCCFH  QQ  FLDSPSK+PT+SPCSW KST  E   +L RC
Sbjct: 1   MLPHFHHSPAFARKLKSSFCCFHGFQQDDFLDSPSKSPTKSPCSWFKSTVGEFRGILARC 60

Query: 61  RYFLARIARRRRKYLASVDFSYDSSSYALNFEDDCRDDHEFPFRDFTARFPPAIKDPVPP 120
           RY LARIARRR++YLASVDF+YDSSSYALNF+DDCR+D EFPFRDFTARFPP IK+  P 
Sbjct: 61  RYLLARIARRRKRYLASVDFTYDSSSYALNFDDDCREDDEFPFRDFTARFPPPIKERAPA 120

Query: 121 PKFAGSQDGIAI 133
            KFAGS+DGI++
Sbjct: 121 AKFAGSEDGISV 132

[Close]

BLAST of Lsi08G012260.1 vs. NCBI nr
Match: XP_008445585.1 (PREDICTED: uncharacterized protein LOC103488565 [Cucumis melo])

HSP 1 Score: 164.1 bits (414), Expect = 8.5e-37
Identity = 84/117 (71.79%), Postives = 89/117 (76.07%), Query Frame = 0


Query: 18  SFCCFHTSQQHQFLDSPSKTPTRSPCSWLKSTAAELSELLYRCRYFLARIARRRRKYLAS 77
           SFCCFH          PS TP  SPCSWLKSTA ELS LL RCRYFLAR+ARRR  YLAS
Sbjct: 5   SFCCFH----------PSNTPKTSPCSWLKSTALELSHLLRRCRYFLARVARRRTNYLAS 64

Query: 78  VDFSYDSSSYALNFEDDCRDDHEFPFRDFTARFPPAIKDPVP-PPKFAGSQDGIAIS 134
            DFSYDSSSYALNF+DDCRDDH+FPFRDFTARFPP +KD +P   KFAGS   I IS
Sbjct: 65  ADFSYDSSSYALNFDDDCRDDHQFPFRDFTARFPPPLKDRLPAATKFAGSHHEIPIS 111

[Close]

BLAST of Lsi08G012260.1 vs. NCBI nr
Match: XP_022131639.1 (uncharacterized protein LOC111004768 [Momordica charantia])

HSP 1 Score: 134.0 bits (336), Expect = 9.5e-28
Identity = 79/137 (57.66%), Postives = 92/137 (67.15%), Query Frame = 0


Query: 1   MLPHFHYSPSLPHKLKSSFCCFHTSQQHQFLDSPSKTPTRSPCSWLKSTAAELSELLYRC 60
           ML  FH+S S  HKLKSS  CF  S      D PS   T+SPCSW KSTAAE  +LL RC
Sbjct: 1   MLADFHHSSSFAHKLKSSIGCFRAS------DQPS---TKSPCSWFKSTAAEFPDLLDRC 60

Query: 61  RYFLARIAR-RRRKYLASVDFSYDSSSYALNFEDDCRDDHEFPFRDFTARFPPAIKDPVP 120
           RY +ARI R  RR+Y AS DF+YDSSSYALNFEDD R D EFP R+F AR P      +P
Sbjct: 61  RYLVARIGRGSRRRYHASPDFTYDSSSYALNFEDDSRYDDEFPLRNFAARLP-----RLP 120

Query: 121 PPKFAGSQDGIA-ISSC 136
             KFAGSQ G++ +++C
Sbjct: 121 AAKFAGSQAGVSTVAAC 123

[Close]

BLAST of Lsi08G012260.1 vs. TAIR 10
Match: AT5G11070.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 62.4 bits (150), Expect = 3.3e-10
Identity = 46/118 (38.98%), Postives = 58/118 (49.15%), Query Frame = 0


Query: 13  HKLKSSFCCFHTS----QQHQFLDSPSKTPTRSPCSWLKSTAAELSELLYRCRYFLARIA 72
           H+++S  CC   +     +   +    +TP RSP  WLKSTA EL EL  RCR   +RI 
Sbjct: 20  HRMRSPICCIGANAVVEPEAMMIGGEQRTP-RSPYEWLKSTAQEL-ELRDRCRRVKSRIK 79

Query: 73  RRRRKYLASV---------------DFSYDSSSYALNFEDDCRDDHEFPFRDFTARFP 112
              R    +                DFSYD  SYALNFED+ R D +  F +FTAR P
Sbjct: 80  VTCRNNNCAYNNCVHHHHHSQSYPGDFSYDPLSYALNFEDNVRADDDGSFPNFTARLP 135

[Close]

BLAST of Lsi08G012260.1 vs. TAIR 10
Match: AT5G35090.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 51.2 bits (121), Expect = 7.5e-07
Identity = 49/149 (32.89%), Postives = 68/149 (45.64%), Query Frame = 0


Query: 10  SLPHKLKSSFC---CFHTSQQHQFLDSPSKTPTRSPCSWLKSTAA--------------- 69
           SL  KLKS FC   CF T+  H   + P   P+ SP +  KST +               
Sbjct: 10  SLQKKLKSRFCIAGCFRTTNHHHHDNIPDDLPS-SPVTTEKSTQSPHGGGMKTKSPRLTR 69

Query: 70  ELSELLYRCRYFLARI----------ARRRRKYLASVDFSYDSSSYALNFEDDCRDDH-- 129
            LS+   +C+  + R+           +  R++ A  DF YD SSYALNF+    DD+  
Sbjct: 70  TLSKSHEKCKSLIHRMGGGVGGVGGHGKHIRRHTA--DFHYDPSSYALNFDKGDEDDNID 129

The following BLAST results are available for this feature:

BLAST of Lsi08G012260.1 vs.


Match Name	E-value	Identity	Description

BLAST of Lsi08G012260.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HE78	2.3e-51	75.76	uncharacterized protein LOC111462741 OS=Cucurbita moschata OX=3662 GN=LOC1114627...	[more]
A0A6J1K7R1	1.9e-50	73.48	uncharacterized protein LOC111492424 OS=Cucurbita maxima OX=3661 GN=LOC111492424...	[more]
A0A1S3BDX7	4.1e-37	71.79	uncharacterized protein LOC103488565 OS=Cucumis melo OX=3656 GN=LOC103488565 PE=...	[more]
A0A6J1BQU1	4.6e-28	57.66	uncharacterized protein LOC111004768 OS=Momordica charantia OX=3673 GN=LOC111004...	[more]
A0A7N2QYM6	4.8e-17	50.00	Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]

BLAST of Lsi08G012260.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022962228.1	4.7e-51	75.76	uncharacterized protein LOC111462741 [Cucurbita moschata] >KAG6598557.1 hypothet...	[more]
XP_023546798.1	2.3e-50	75.00	uncharacterized protein LOC111805797 [Cucurbita pepo subsp. pepo]	[more]
XP_022997531.1	4.0e-50	73.48	uncharacterized protein LOC111492424 [Cucurbita maxima]	[more]
XP_008445585.1	8.5e-37	71.79	PREDICTED: uncharacterized protein LOC103488565 [Cucumis melo]	[more]
XP_022131639.1	9.5e-28	57.66	uncharacterized protein LOC111004768 [Momordica charantia]	[more]

BLAST of Lsi08G012260.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G11070.1	3.3e-10	38.98	unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0...	[more]
AT5G35090.1	7.5e-07	32.89	unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33168	STRESS INDUCED PROTEIN-RELATED	coord: 11..113
None	No IPR available	PANTHER	PTHR33168:SF61	SUBFAMILY NOT NAMED	coord: 11..113

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi08G012260	Lsi08G012260	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G012260.1.exon.1	Lsi08G012260.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G012260.1.CDS.1	Lsi08G012260.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi08G012260.1	Lsi08G012260.1-protein	polypeptide