Lsi05G012420.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G012420.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	3-hexulose-6-phosphate isomerase
Location	chr05: 20238810 .. 20240572 (+)
Sequence length	663
RNA-Seq Expression	Lsi05G012420.1
Synteny	Lsi05G012420.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAGTGAAGTAAAACAACGACAACTTCACCAATTGAAATTTTTTGTTTCTAATGCAGTTTCCTCTGCTTTCAACAAAATATGAAGTTCATCAAACAAAATTGTTTGAGATCTTGTTTGAAAAGAGGTTTTAAAAACATTTTAAGAAAACACAAACACGTTCATAGTATAAATGTTCAGATCTTCTGCATTAATAACAACTAAAACAACTACTAATTTGTTAAGTGTCTCTTTAAATCTTCGAACATATGCATCAATTGACCCATCATTCTTTTTTAAAATATTTTGCAACTCAGCCTTGAGGTTCACAATATTCATTTGACAGGAAGATGAAACATGTTTCTCTAAAGTTTCTCATATTTTTTTAGCAATTTGATACCCAATCACGTAGGAGAGAGTTGTATGTGTCAGTGTCACATTAATGAGAGTAATTAGGGCTTGATCTTTGTTGTACCATTGTTCATACTTAGGGTTTGCCTCAAGAGGTTGCCCTTCAACTCACATAACTTTGACTGGAACAGTATAAAAACCATCAACAAAACGATAAAGTTTGTGTGATTTGAGAAGATGAGAGATTTGAAGCCGTCATAGTATATAATTTGTTGTATCAAGACAAACTGAGATCAAGTTGTAAATATTCGAGAGCAGCGTCAATGAGGTATTTTGATTATGATCTTCATATTGCATGATGACCAAGAAATATTTCAAGAGAGAGAGGGAGGTCTTAATATGCACAAGTGTTTTGACATTTGTCACGAGACTCTGATACCAAAAAGATAAATAGAGTTTTGAAAAACTCAGCAACTGATTACATTATACTCTCTTAGAGTGTGTTTGGTCCAAGAAGTTGGAAAATAAGAGTCATGAACTCCACTTATTGTTTGGCCCAAAGAGTTGATGAGTCATATTACTAAAAAAATATATTGATTTTATATCTTATTAACTCCTTACTTTAAAGGCTCTAATAATTTAGAAGTCTCTAGACTTCATAACTTTTTACTTCTAGTTTACACACACGTGGCATGATACCACAAGATTACCCAAAACGATTGCACCACAAGCGCAACACACAGAGACAAAACCCCTTGATTTCGTTAGTTTAAAGAAGATCCGTTGAAACTAACAGAGAGTCTCCATCAAGCTCTCGAAAATCCCATATCAATGGCCTCCCAATCACAGGCTTCTCTTGCCTCCCGAATATGCTCAGAAATTGCTTCCATCTTCTCCAAACCCACGCAACGCCACGCGCCGCCGCTGGACCTTTTGGTCTCTGAGCTCTCCACCGCCGCCGCACGCAAGGCCCGGGTGTTCCTTTACGGCGTCGGCCGTGAGGGCCTAATGCTCAAAACCCTCTGTATGCGGCTCGCTCATCTCGGCCTCTCGTCGCACCAGGTCTTCGACATGAACGCGCCCCCAATCGCCGCTCCTGACCTCCTAATTGCGTCAGCCGGCCCCGGCGGATTTTCGACCGTCGATGCGATTTGCTCCGTGGCCAGATCCAACGGCGGTAGAGTTTTGCTTCTGACGGCCCAGCCGGAGAACGGCTCGTCTGTGAAGCACGCCGATGTGATTTGCTACGTTCCGGCGCAGACCATGGCGGACGATAAGAAATCGGCGGCGGAATGTCGGCCTCTGCTTCCGATGGGGAGTTTGTATGAAGGAGCTCTGTTTGTTTTGTTTGAGATGGTGGTGTATCGGTTGGGTGAAGCGTTGGGAGAGAGCCCTGAGGCCGTCCGATCACGCCACACCAATCTTGAATGA

mRNA sequence

ATGGCAAAAGATCCGTTGAAACTAACAGAGAGTCTCCATCAAGCTCTCGAAAATCCCATATCAATGGCCTCCCAATCACAGGCTTCTCTTGCCTCCCGAATATGCTCAGAAATTGCTTCCATCTTCTCCAAACCCACGCAACGCCACGCGCCGCCGCTGGACCTTTTGGTCTCTGAGCTCTCCACCGCCGCCGCACGCAAGGCCCGGGTGTTCCTTTACGGCGTCGGCCGTGAGGGCCTAATGCTCAAAACCCTCTGTATGCGGCTCGCTCATCTCGGCCTCTCGTCGCACCAGGTCTTCGACATGAACGCGCCCCCAATCGCCGCTCCTGACCTCCTAATTGCGTCAGCCGGCCCCGGCGGATTTTCGACCGTCGATGCGATTTGCTCCGTGGCCAGATCCAACGGCGGTAGAGTTTTGCTTCTGACGGCCCAGCCGGAGAACGGCTCGTCTGTGAAGCACGCCGATGTGATTTGCTACGTTCCGGCGCAGACCATGGCGGACGATAAGAAATCGGCGGCGGAATGTCGGCCTCTGCTTCCGATGGGGAGTTTGTATGAAGGAGCTCTGTTTGTTTTGTTTGAGATGGTGGTGTATCGGTTGGGTGAAGCGTTGGGAGAGAGCCCTGAGGCCGTCCGATCACGCCACACCAATCTTGAATGA

Coding sequence (CDS)

ATGGCAAAAGATCCGTTGAAACTAACAGAGAGTCTCCATCAAGCTCTCGAAAATCCCATATCAATGGCCTCCCAATCACAGGCTTCTCTTGCCTCCCGAATATGCTCAGAAATTGCTTCCATCTTCTCCAAACCCACGCAACGCCACGCGCCGCCGCTGGACCTTTTGGTCTCTGAGCTCTCCACCGCCGCCGCACGCAAGGCCCGGGTGTTCCTTTACGGCGTCGGCCGTGAGGGCCTAATGCTCAAAACCCTCTGTATGCGGCTCGCTCATCTCGGCCTCTCGTCGCACCAGGTCTTCGACATGAACGCGCCCCCAATCGCCGCTCCTGACCTCCTAATTGCGTCAGCCGGCCCCGGCGGATTTTCGACCGTCGATGCGATTTGCTCCGTGGCCAGATCCAACGGCGGTAGAGTTTTGCTTCTGACGGCCCAGCCGGAGAACGGCTCGTCTGTGAAGCACGCCGATGTGATTTGCTACGTTCCGGCGCAGACCATGGCGGACGATAAGAAATCGGCGGCGGAATGTCGGCCTCTGCTTCCGATGGGGAGTTTGTATGAAGGAGCTCTGTTTGTTTTGTTTGAGATGGTGGTGTATCGGTTGGGTGAAGCGTTGGGAGAGAGCCCTGAGGCCGTCCGATCACGCCACACCAATCTTGAATGA

Protein sequence

MAKDPLKLTESLHQALENPISMASQSQASLASRICSEIASIFSKPTQRHAPPLDLLVSELSTAAARKARVFLYGVGREGLMLKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVLLLTAQPENGSSVKHADVICYVPAQTMADDKKSAAECRPLLPMGSLYEGALFVLFEMVVYRLGEALGESPEAVRSRHTNLE

Homology

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BLAST of Lsi05G012420.1 vs. ExPASy Swiss-Prot
Match: P42404 (3-hexulose-6-phosphate isomerase OS=Bacillus subtilis (strain 168) OX=224308 GN=hxlB PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.3e-17
Identity = 54/152 (35.53%), Postives = 84/152 (55.26%), Query Frame = 0


Query: 69  RVFLYGVGREGLMLKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAI 128
           ++F  G GR GLM K+  MRL H+G ++H V ++  PP+A  DL+I  +G G   ++   
Sbjct: 38  QIFTAGAGRSGLMAKSFAMRLMHMGFNAHIVGEILTPPLAEGDLVIIGSGSGETKSLIHT 97

Query: 129 CSVARSNGGRVLLLTAQPENGSSVKHADVICYVPAQTMADDKKSAAECRPLLPMGSLYEG 188
            + A+S  G V  LT  PE+ S  K AD+I  +P    +   +S    + + PMGSL+E 
Sbjct: 98  AAKAKSLHGIVAALTINPES-SIGKQADLIIRMPG---SPKDQSNGSYKTIQPMGSLFEQ 157

Query: 189 ALFVLFEMVVYRLGEALGESPEAVRSRHTNLE 221
            L + ++ V+ +L E  G   E + + H NLE
Sbjct: 158 TLLLFYDAVILKLMEKKGLDSETMFTHHANLE 185

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BLAST of Lsi05G012420.1 vs. ExPASy Swiss-Prot
Match: Q9YAK0 (Uncharacterized protein APE_1940.1 OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=APE_1940.1 PE=3 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 8.2e-16
Identity = 53/174 (30.46%), Postives = 86/174 (49.43%), Query Frame = 0


Query: 53  LDLLVSELSTAAARKARVFLYGVGREGLMLKTLCMRLAHLGLSSHQVFDMNAPPIAAPDL 112
           +D  V EL      K +V + G GR GL+ K   MRL HLG +S+ + +   P +   DL
Sbjct: 39  VDRFVGELERVYREKRKVLVMGAGRSGLVGKAFAMRLLHLGFNSYVLGETIVPSVREGDL 98

Query: 113 LIASAGPGGFSTVDAICSVARSNGGRVLLLTAQPENGSSVKHADVICYVPAQTMA---DD 172
           ++A +G G    +      A+  G  V  +T  P++    + +D++  VP +T +   DD
Sbjct: 99  VVAISGSGRTKVIVTAAETAKQVGATVAAITTYPDSPLG-RLSDIVVRVPGRTKSSKMDD 158

Query: 173 ---KKSAAECRPLLPMGSLYEGALFVLFEMVVYRLGEALGESPEAVRSRHTNLE 221
              ++      PL P+G+L+E    V  + V+Y L   LG   E +R+ H N+E
Sbjct: 159 YFARQILGIHEPLAPLGTLFEDTTMVFLDGVIYSLMTRLGIDEEYMRNMHANVE 211

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BLAST of Lsi05G012420.1 vs. ExPASy Swiss-Prot
Match: Q9LBW5 (3-hexulose-6-phosphate isomerase OS=Mycobacterium gastri OX=1777 GN=rmpB PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 5.3e-15
Identity = 55/164 (33.54%), Postives = 78/164 (47.56%), Query Frame = 0


Query: 57  VSELSTAAARKARVFLYGVGREGLMLKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIAS 116
           V+ L+    +  RVF+ G GR GL+L+   MRL H GL+ H   D   P I+A DLL+ +
Sbjct: 41  VAVLARQIVQPGRVFVAGAGRSGLVLRMAAMRLMHFGLTVHVAGDTTTPAISAGDLLLVA 100

Query: 117 AGPGGFSTVDAICSVARSNGGRVLLLTAQPENGSSVKHADVICYVPAQTMADDKKSAAEC 176
           +G G  S V      A+  G R+   T  P++      AD +  +PA    D     +  
Sbjct: 101 SGSGTTSGVVKSAETAKKAGARIAAFTTNPDS-PLAGLADAVVIIPAAQKTDHGSHISR- 160

Query: 177 RPLLPMGSLYEGALFVLFEMVVYRLGEALGESPEAVRSRHTNLE 221
                 GSL+E  LFV+ E V   L +      E + +RH NLE
Sbjct: 161 ---QYAGSLFEQVLFVVTEAVFQSLWDHTEVEAEELWTRHANLE 199

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BLAST of Lsi05G012420.1 vs. ExPASy Swiss-Prot
Match: Q58644 (3-hexulose-6-phosphate isomerase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=phi PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 8.8e-10
Identity = 47/167 (28.14%), Postives = 79/167 (47.31%), Query Frame = 0


Query: 53  LDLLVSELSTAAARKARVFLYGVGREGLMLKTLCMRLAHLGLSSHQVFDMNAPPIAAPDL 112
           LD L+  +  A     ++F++GVGR G + +   MRL HLG  S+ V +   P     DL
Sbjct: 30  LDSLIDRIIKA----KKIFIFGVGRSGYIGRCFAMRLMHLGFKSYFVGETTTPSYEKDDL 89

Query: 113 LIASAGPGGFSTVDAICSVARSNGGRVLLLTAQPENGSSVKHADVICYVPAQTMADDKKS 172
           LI  +G G   +V  +   A++    ++ +    E G+ V+ AD+   +P +     KKS
Sbjct: 90  LILISGSGRTESVLTVAKKAKNINNNIIAIVC--ECGNVVEFADLT--IPLEV----KKS 149

Query: 173 AAECRPLLPMGSLYEGALFVLFEMVVYRLGEALGESPEAVRSRHTNL 220
                  LPMG+ +E    +  ++V+  + + L      +  RH NL
Sbjct: 150 -----KYLPMGTTFEETALIFLDLVIAEIMKRLNLDESEIIKRHCNL 179

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BLAST of Lsi05G012420.1 vs. ExPASy TrEMBL
Match: A0A5A7SW26 (3-hexulose-6-phosphate isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00080 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.0e-93
Identity = 184/201 (91.54%), Postives = 191/201 (95.02%), Query Frame = 0


Query: 22  MASQSQASLASRICSEIASIFSKPTQRHAPPLDLLVSELSTAAARKARVFLYGVGREGLM 81
           MASQS ASLASRICSEIASIFSKPTQ +AP LD+LVSELS+ AARKARVFLYGVGREGLM
Sbjct: 1   MASQSPASLASRICSEIASIFSKPTQPYAPSLDILVSELSSVAARKARVFLYGVGREGLM 60

Query: 82  LKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVLL 141
           LK LCMRLAHLGLSSHQVFDMNAPPIA  DLLIASAGPGGFSTVDAICSVARSNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAGSDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 142 LTAQPENGSSVKHADVICYVPAQTMADDKKS--AAECRPLLPMGSLYEGALFVLFEMVVY 201
           LTAQPENGSSVK+ADVICYVPAQTMADD++S  AAECRPLLPMGSLYEGALFVLFEMVVY
Sbjct: 121 LTAQPENGSSVKYADVICYVPAQTMADDEESAAAAECRPLLPMGSLYEGALFVLFEMVVY 180

Query: 202 RLGEALGESPEAVRSRHTNLE 221
           R+GE LGESPEAVRSRHTNLE
Sbjct: 181 RIGEVLGESPEAVRSRHTNLE 201

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BLAST of Lsi05G012420.1 vs. ExPASy TrEMBL
Match: A0A6J1G183 (uncharacterized protein LOC111449742 OS=Cucurbita moschata OX=3662 GN=LOC111449742 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 5.0e-93
Identity = 182/203 (89.66%), Postives = 191/203 (94.09%), Query Frame = 0


Query: 22  MASQSQASLASRICSEIASIFSKPTQRHAPPLDLLVSELSTAAARKARVFLYGVGREGLM 81
           MASQS  SLASRICSEIAS+FSKPT++H+PPL+LLVSELS AAARKARVFLYGVGREGLM
Sbjct: 1   MASQSPPSLASRICSEIASVFSKPTRQHSPPLELLVSELSAAAARKARVFLYGVGREGLM 60

Query: 82  LKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVLL 141
           LK LCMRLAHLGLSSHQVFDMNAPPIAA DLLIASAGPGGFSTVDAICSVARSNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAHDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 142 LTAQPENGSSVKHADVICYVPAQTMADDKKS----AAECRPLLPMGSLYEGALFVLFEMV 201
           LTAQPE GSSVKHADVICYVPAQTMADD++S    A +CRPLLPMGSLYEGALFVLFEMV
Sbjct: 121 LTAQPETGSSVKHADVICYVPAQTMADDEESMTETAVKCRPLLPMGSLYEGALFVLFEMV 180

Query: 202 VYRLGEALGESPEAVRSRHTNLE 221
           VY+L EALGESPEAVRSRHTNLE
Sbjct: 181 VYQLAEALGESPEAVRSRHTNLE 203

[Close]

BLAST of Lsi05G012420.1 vs. ExPASy TrEMBL
Match: A0A1S3AXT7 (3-hexulose-6-phosphate isomerase OS=Cucumis melo OX=3656 GN=LOC103484066 PE=4 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 6.6e-93
Identity = 183/201 (91.04%), Postives = 190/201 (94.53%), Query Frame = 0


Query: 22  MASQSQASLASRICSEIASIFSKPTQRHAPPLDLLVSELSTAAARKARVFLYGVGREGLM 81
           MASQS ASLASRICSEIASIFSKPTQ +AP LD+LVSELS+ AARKARVFLYGVGREGLM
Sbjct: 1   MASQSPASLASRICSEIASIFSKPTQPYAPSLDILVSELSSVAARKARVFLYGVGREGLM 60

Query: 82  LKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVLL 141
           LK LCMRLAHLGLSSHQVFDMNAPPIA  DLLIASAGPGGFSTVDAICSVARSNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAGSDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 142 LTAQPENGSSVKHADVICYVPAQTMADDKKS--AAECRPLLPMGSLYEGALFVLFEMVVY 201
           LTAQPENGSSVK+ADVICYVPAQTMADD++S  AAECRPLLPMGSLYEGALFVLFEMVVY
Sbjct: 121 LTAQPENGSSVKYADVICYVPAQTMADDEESAAAAECRPLLPMGSLYEGALFVLFEMVVY 180

Query: 202 RLGEALGESPEAVRSRHTNLE 221
           R+G  LGESPEAVRSRHTNLE
Sbjct: 181 RIGVVLGESPEAVRSRHTNLE 201

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BLAST of Lsi05G012420.1 vs. ExPASy TrEMBL
Match: A0A6J1HTB3 (uncharacterized protein LOC111467597 OS=Cucurbita maxima OX=3661 GN=LOC111467597 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.5e-92
Identity = 181/203 (89.16%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 22  MASQSQASLASRICSEIASIFSKPTQRHAPPLDLLVSELSTAAARKARVFLYGVGREGLM 81
           MASQS  SLASRICSEIAS+F KPT++H+PPL+LLVSELS AAARKARVFLYGVGREGLM
Sbjct: 1   MASQSPPSLASRICSEIASVFFKPTRQHSPPLELLVSELSAAAARKARVFLYGVGREGLM 60

Query: 82  LKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVLL 141
           LK LCMRLAHLGLSSHQVFDMNAPPIAA DLLIASAGPGGFSTVDAICSVARSNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAARDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 142 LTAQPENGSSVKHADVICYVPAQTMADDKKS----AAECRPLLPMGSLYEGALFVLFEMV 201
           LTAQPE GSSVKHADVICYVPAQTMADD++S    A +CRPLLPMGSLYEGALFVLFEMV
Sbjct: 121 LTAQPETGSSVKHADVICYVPAQTMADDEESMTETAVKCRPLLPMGSLYEGALFVLFEMV 180

Query: 202 VYRLGEALGESPEAVRSRHTNLE 221
           VY+L EALGESPEAVRSRHTNLE
Sbjct: 181 VYQLAEALGESPEAVRSRHTNLE 203

[Close]

BLAST of Lsi05G012420.1 vs. ExPASy TrEMBL
Match: A0A0A0LBC8 (3-hexulose-6-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_3G181970 PE=3 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 2.8e-91
Identity = 180/201 (89.55%), Postives = 189/201 (94.03%), Query Frame = 0


Query: 22  MASQSQASLASRICSEIASIFSKPTQRHAPPLDLLVSELSTAAARKARVFLYGVGREGLM 81
           MASQS ASLASRICSEIASIFSKPTQ +APPL++LVSELS+ A RKARVFLYGVGREGLM
Sbjct: 1   MASQSPASLASRICSEIASIFSKPTQPYAPPLEILVSELSSVAERKARVFLYGVGREGLM 60

Query: 82  LKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVLL 141
           LK LCMRLAHLGLSSHQVFDMNAPPIA  DLLIASAGPGGFSTVDAICSVARS GGRVL+
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAGSDLLIASAGPGGFSTVDAICSVARSKGGRVLV 120

Query: 142 LTAQPENGSSVKHADVICYVPAQTMADDKKS--AAECRPLLPMGSLYEGALFVLFEMVVY 201
           LTAQPENGSSVK+ADVICYVPAQTMADD++S  AAE RPLLPMGSLYEGALFVLFEMVVY
Sbjct: 121 LTAQPENGSSVKYADVICYVPAQTMADDEESGEAAEWRPLLPMGSLYEGALFVLFEMVVY 180

Query: 202 RLGEALGESPEAVRSRHTNLE 221
           R+GE LGESPEAVRSRHTNLE
Sbjct: 181 RIGEVLGESPEAVRSRHTNLE 201

[Close]

BLAST of Lsi05G012420.1 vs. NCBI nr
Match: XP_038901080.1 (3-hexulose-6-phosphate isomerase [Benincasa hispida])

HSP 1 Score: 365.9 bits (938), Expect = 2.4e-97
Identity = 190/200 (95.00%), Postives = 196/200 (98.00%), Query Frame = 0


Query: 22  MASQSQA-SLASRICSEIASIFSKPTQRHAPPLDLLVSELSTAAARKARVFLYGVGREGL 81
           MASQSQA SLASRICS+IASIFSKPTQ HAPPLDLLVSELS+AAARKARVFLYGVGREGL
Sbjct: 1   MASQSQASSLASRICSDIASIFSKPTQPHAPPLDLLVSELSSAAARKARVFLYGVGREGL 60

Query: 82  MLKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVL 141
           MLK LCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVL
Sbjct: 61  MLKALCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVL 120

Query: 142 LLTAQPENGSSVKHADVICYVPAQTMADDKKSAAECRPLLPMGSLYEGALFVLFEMVVYR 201
           LLTAQPENGSSVK+ADVICYVPAQTMADD++SAA+CRPLLPMGSLYEGALFVLFEMVVYR
Sbjct: 121 LLTAQPENGSSVKYADVICYVPAQTMADDEESAAKCRPLLPMGSLYEGALFVLFEMVVYR 180

Query: 202 LGEALGESPEAVRSRHTNLE 221
           LGE LGESPEAVRSRHTNLE
Sbjct: 181 LGEVLGESPEAVRSRHTNLE 200

[Close]

BLAST of Lsi05G012420.1 vs. NCBI nr
Match: KAA0033631.1 (3-hexulose-6-phosphate isomerase [Cucumis melo var. makuwa] >TYK22930.1 3-hexulose-6-phosphate isomerase [Cucumis melo var. makuwa])

HSP 1 Score: 352.8 bits (904), Expect = 2.1e-93
Identity = 184/201 (91.54%), Postives = 191/201 (95.02%), Query Frame = 0


Query: 22  MASQSQASLASRICSEIASIFSKPTQRHAPPLDLLVSELSTAAARKARVFLYGVGREGLM 81
           MASQS ASLASRICSEIASIFSKPTQ +AP LD+LVSELS+ AARKARVFLYGVGREGLM
Sbjct: 1   MASQSPASLASRICSEIASIFSKPTQPYAPSLDILVSELSSVAARKARVFLYGVGREGLM 60

Query: 82  LKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVLL 141
           LK LCMRLAHLGLSSHQVFDMNAPPIA  DLLIASAGPGGFSTVDAICSVARSNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAGSDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 142 LTAQPENGSSVKHADVICYVPAQTMADDKKS--AAECRPLLPMGSLYEGALFVLFEMVVY 201
           LTAQPENGSSVK+ADVICYVPAQTMADD++S  AAECRPLLPMGSLYEGALFVLFEMVVY
Sbjct: 121 LTAQPENGSSVKYADVICYVPAQTMADDEESAAAAECRPLLPMGSLYEGALFVLFEMVVY 180

Query: 202 RLGEALGESPEAVRSRHTNLE 221
           R+GE LGESPEAVRSRHTNLE
Sbjct: 181 RIGEVLGESPEAVRSRHTNLE 201

[Close]

BLAST of Lsi05G012420.1 vs. NCBI nr
Match: XP_022945534.1 (uncharacterized protein LOC111449742 [Cucurbita moschata] >XP_023542323.1 uncharacterized protein LOC111802255 [Cucurbita pepo subsp. pepo] >KAG6574169.1 hypothetical protein SDJN03_28056, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013223.1 hxlB, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 350.5 bits (898), Expect = 1.0e-92
Identity = 182/203 (89.66%), Postives = 191/203 (94.09%), Query Frame = 0


Query: 22  MASQSQASLASRICSEIASIFSKPTQRHAPPLDLLVSELSTAAARKARVFLYGVGREGLM 81
           MASQS  SLASRICSEIAS+FSKPT++H+PPL+LLVSELS AAARKARVFLYGVGREGLM
Sbjct: 1   MASQSPPSLASRICSEIASVFSKPTRQHSPPLELLVSELSAAAARKARVFLYGVGREGLM 60

Query: 82  LKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVLL 141
           LK LCMRLAHLGLSSHQVFDMNAPPIAA DLLIASAGPGGFSTVDAICSVARSNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAHDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 142 LTAQPENGSSVKHADVICYVPAQTMADDKKS----AAECRPLLPMGSLYEGALFVLFEMV 201
           LTAQPE GSSVKHADVICYVPAQTMADD++S    A +CRPLLPMGSLYEGALFVLFEMV
Sbjct: 121 LTAQPETGSSVKHADVICYVPAQTMADDEESMTETAVKCRPLLPMGSLYEGALFVLFEMV 180

Query: 202 VYRLGEALGESPEAVRSRHTNLE 221
           VY+L EALGESPEAVRSRHTNLE
Sbjct: 181 VYQLAEALGESPEAVRSRHTNLE 203

[Close]

BLAST of Lsi05G012420.1 vs. NCBI nr
Match: XP_008439193.1 (PREDICTED: 3-hexulose-6-phosphate isomerase [Cucumis melo])

HSP 1 Score: 350.1 bits (897), Expect = 1.4e-92
Identity = 183/201 (91.04%), Postives = 190/201 (94.53%), Query Frame = 0


Query: 22  MASQSQASLASRICSEIASIFSKPTQRHAPPLDLLVSELSTAAARKARVFLYGVGREGLM 81
           MASQS ASLASRICSEIASIFSKPTQ +AP LD+LVSELS+ AARKARVFLYGVGREGLM
Sbjct: 1   MASQSPASLASRICSEIASIFSKPTQPYAPSLDILVSELSSVAARKARVFLYGVGREGLM 60

Query: 82  LKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVLL 141
           LK LCMRLAHLGLSSHQVFDMNAPPIA  DLLIASAGPGGFSTVDAICSVARSNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAGSDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 142 LTAQPENGSSVKHADVICYVPAQTMADDKKS--AAECRPLLPMGSLYEGALFVLFEMVVY 201
           LTAQPENGSSVK+ADVICYVPAQTMADD++S  AAECRPLLPMGSLYEGALFVLFEMVVY
Sbjct: 121 LTAQPENGSSVKYADVICYVPAQTMADDEESAAAAECRPLLPMGSLYEGALFVLFEMVVY 180

Query: 202 RLGEALGESPEAVRSRHTNLE 221
           R+G  LGESPEAVRSRHTNLE
Sbjct: 181 RIGVVLGESPEAVRSRHTNLE 201

[Close]

BLAST of Lsi05G012420.1 vs. NCBI nr
Match: XP_022968332.1 (uncharacterized protein LOC111467597 [Cucurbita maxima])

HSP 1 Score: 348.2 bits (892), Expect = 5.2e-92
Identity = 181/203 (89.16%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 22  MASQSQASLASRICSEIASIFSKPTQRHAPPLDLLVSELSTAAARKARVFLYGVGREGLM 81
           MASQS  SLASRICSEIAS+F KPT++H+PPL+LLVSELS AAARKARVFLYGVGREGLM
Sbjct: 1   MASQSPPSLASRICSEIASVFFKPTRQHSPPLELLVSELSAAAARKARVFLYGVGREGLM 60

Query: 82  LKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVLL 141
           LK LCMRLAHLGLSSHQVFDMNAPPIAA DLLIASAGPGGFSTVDAICSVARSNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAARDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 142 LTAQPENGSSVKHADVICYVPAQTMADDKKS----AAECRPLLPMGSLYEGALFVLFEMV 201
           LTAQPE GSSVKHADVICYVPAQTMADD++S    A +CRPLLPMGSLYEGALFVLFEMV
Sbjct: 121 LTAQPETGSSVKHADVICYVPAQTMADDEESMTETAVKCRPLLPMGSLYEGALFVLFEMV 180

Query: 202 VYRLGEALGESPEAVRSRHTNLE 221
           VY+L EALGESPEAVRSRHTNLE
Sbjct: 181 VYQLAEALGESPEAVRSRHTNLE 203

[Close]

BLAST of Lsi05G012420.1 vs. TAIR 10
Match: AT5G52190.1 (Sugar isomerase (SIS) family protein )

HSP 1 Score: 257.7 bits (657), Expect = 8.6e-69
Identity = 131/203 (64.53%), Postives = 161/203 (79.31%), Query Frame = 0


Query: 24  SQSQASLASRICSEIASIFSKPTQRHAPPLDLLVSELSTAAARK--ARVFLYGVGREGLM 83
           +Q  A+L+S+IC++I+S+F+KPT  + PPLDLLV+EL+  +  +  +R+FLYGVGREGLM
Sbjct: 6   AQEMANLSSQICNQISSVFTKPTSPYPPPLDLLVTELTAISRNRNNSRIFLYGVGREGLM 65

Query: 84  LKTLCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVLL 143
           LK   MRL H GL +H VFDM  PPI++ DLLIASAGPGGFSTVDA+CSVA+SNG +V+L
Sbjct: 66  LKAFAMRLFHFGLPTHLVFDMTTPPISSSDLLIASAGPGGFSTVDALCSVAKSNGAKVIL 125

Query: 144 LTAQPENGSSVKHADVICYVPAQTMADDKKSAAEC----RPLLPMGSLYEGALFVLFEMV 203
           +TA+P  GS VKHA  +CYVPAQTMA D           R LLPMGS+YEGALFVLFEMV
Sbjct: 126 ITAEPAIGSCVKHATDVCYVPAQTMASDNGGGDSVEMGERRLLPMGSVYEGALFVLFEMV 185

Query: 204 VYRLGEALGESPEAVRSRHTNLE 221
           VY+LGE LGES E+VR+RHTNLE
Sbjct: 186 VYKLGEVLGESSESVRARHTNLE 208

The following BLAST results are available for this feature:

BLAST of Lsi05G012420.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42404	3.3e-17	35.53	3-hexulose-6-phosphate isomerase OS=Bacillus subtilis (strain 168) OX=224308 GN=...	[more]
Q9YAK0	8.2e-16	30.46	Uncharacterized protein APE_1940.1 OS=Aeropyrum pernix (strain ATCC 700893 / DSM...	[more]
Q9LBW5	5.3e-15	33.54	3-hexulose-6-phosphate isomerase OS=Mycobacterium gastri OX=1777 GN=rmpB PE=2 SV...	[more]
Q58644	8.8e-10	28.14	3-hexulose-6-phosphate isomerase OS=Methanocaldococcus jannaschii (strain ATCC 4...	[more]

BLAST of Lsi05G012420.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SW26	1.0e-93	91.54	3-hexulose-6-phosphate isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A6J1G183	5.0e-93	89.66	uncharacterized protein LOC111449742 OS=Cucurbita moschata OX=3662 GN=LOC1114497...	[more]
A0A1S3AXT7	6.6e-93	91.04	3-hexulose-6-phosphate isomerase OS=Cucumis melo OX=3656 GN=LOC103484066 PE=4 SV...	[more]
A0A6J1HTB3	2.5e-92	89.16	uncharacterized protein LOC111467597 OS=Cucurbita maxima OX=3661 GN=LOC111467597...	[more]
A0A0A0LBC8	2.8e-91	89.55	3-hexulose-6-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_3G181970 PE=3...	[more]

BLAST of Lsi05G012420.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901080.1	2.4e-97	95.00	3-hexulose-6-phosphate isomerase [Benincasa hispida]	[more]
KAA0033631.1	2.1e-93	91.54	3-hexulose-6-phosphate isomerase [Cucumis melo var. makuwa] >TYK22930.1 3-hexulo...	[more]
XP_022945534.1	1.0e-92	89.66	uncharacterized protein LOC111449742 [Cucurbita moschata] >XP_023542323.1 unchar...	[more]
XP_008439193.1	1.4e-92	91.04	PREDICTED: 3-hexulose-6-phosphate isomerase [Cucumis melo]	[more]
XP_022968332.1	5.2e-92	89.16	uncharacterized protein LOC111467597 [Cucurbita maxima]	[more]

BLAST of Lsi05G012420.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52190.1	8.6e-69	64.53	Sugar isomerase (SIS) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.10490		coord: 26..220 e-value: 2.6E-34 score: 120.3
None	No IPR available	SUPERFAMILY	53697	SIS domain	coord: 53..219
IPR017552	3-hexulose-6-phosphate isomerase	PANTHER	PTHR43443	3-HEXULOSE-6-PHOSPHATE ISOMERASE	coord: 22..220
IPR017552	3-hexulose-6-phosphate isomerase	CDD	cd05005	SIS_PHI	coord: 31..217 e-value: 9.7E-63 score: 191.25

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi05G012420	Lsi05G012420	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi05G012420.1.exon.1	Lsi05G012420.1.exon.1	exon
Lsi05G012420.1.exon.2	Lsi05G012420.1.exon.2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi05G012420.1.CDS.1	Lsi05G012420.1.CDS.1	CDS
Lsi05G012420.1.CDS.2	Lsi05G012420.1.CDS.2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi05G012420.1	Lsi05G012420.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1901135	carbohydrate derivative metabolic process
molecular_function	GO:0016853	isomerase activity
molecular_function	GO:0097367	carbohydrate derivative binding