Lsi03G022080.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G022080.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING-type domain-containing protein
Location	chr03: 33094073 .. 33096293 (-)
Sequence length	1130
RNA-Seq Expression	Lsi03G022080.1
Synteny	Lsi03G022080.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGAAAGCCAAGTGAGTCCCACTTCCCTTCTCTACGGCTGTTTTTCACTTCTCGAATTGCCGAATTTGCTTCGAAAGCCCTTTGGCGAAGAACTACACACAGAAAGGGGATTTCATAATTTCATATCTTGGATTTCTTTTAGAGGTGCGCCGTTGAAGACGAACGAGGTGTAAGGAATTTTCGCTTGCAGATTGCATAGGTATTTATTTTTGTTGTTTTTGAGTTTCGTTTTTTGTGTTCTTTATGGTTTATTCTGAATTGAATGGAAATTTATCTATTGAATTGAATTAGATGAGGTAGGAGTTGTTGCTGCTGATACTGGAAGTGGTGGATGGGAGTTTCTAAGTGTGGAAATTAAGTCAAAATGCGGAAATCTTTCAAGGATTCCCTCAAGGCACTCGAGGCTGATATTCAGTTCGCCAATACCTTGTAATCTCTTCATATGCGGTTCAACTTTCTTATAATTTGATATTTACTCTGGATTCTGTGAGAATTGCTAATGCAATGTTTGTTTCGTTGTCTGGAAATTATCAGCTATGTTTGTTAGATTTCTATGATCGAAACTGAATCTAGTTTCAATGTTTTGTATCTTATGATCTATGTTTTCTCTATGCTTAGATTAGAATGTCAGTCATGTTGGTTTCGTTGCGTATAGAGTTTGAGGTTCAGGAAAGTTTCTCTGAAAGTAGTTCGATAGGTTATGCTGTCTGAGAGAGCTGCAAGTTTTTAGGTTTTGCATCTCATTACCTGTACATTCTTCCTTTCTTATTAGATTAGATATTAGTCGCTTCGATCTTATTCGTGGAATTGTTCTGCGCCCACTCAAATACGTTGTTTCCTGCTCTAAGATTTATGGAGCTATTTATTTAAGATTTAATAATAACTTTTGTGTTTCTTATTTTATAAATGTTGAGTAATTTGGATATGTATTTTCTTCCTTTTGTGTGGGTTATGTTGTATGCTTAGGTGGTTTGTGAACTTTCGCATTGTCCAGCTAGTAGTTTTGTAAGATATCTTGACTTCTCAGGATTGTATGACAATTATAGCCTTGAACTGCAACATATATTTTCTTTCAAATTGGAGATTTTTGTTGTTTTTAGTGTGCAATAGTTCCGGTTGAGGCTTATTTAATTCATAAGCATTGAAATAAAACACTTCAAATCTGAACAGGGCTTCTGATTATCCCAAGGAATATGATGGAGCATGCCTACAAATGAGATTATCCTATAGCCCGGCTGCACAGTTCTTTCTCTTCTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGTCTGCTCAGAATTCTGATATATAAGGTACTGCAGCCTGCATGAGAAATGACATCTTTGACTCCTACATTGTCCAAGTGTGGTGATTGAAGCCGATCACTAAAGATAAAAATCTGATGAAGTAGCTTATACTACTTGCCTCTACACATATCTTGTAAATCCTGTGAATGTGATGAATTTGTTTCAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGTAAGTGGCTAAGAAGATAAAAGCTGCCCGTTCTTCAACTTAAGGTGCATTAAAATTTGGAGTCTTGCTAATCCTATATTTTAGTTCTGCAAATACAGGGGTGATATTTCCTTCTCTAGTGCAACTTCAAAGAGGAATTACCGACATAGAAGAGAGAAAACAGAGAGAAGTTTATGCTGCAAAATTCAAAAGAAAGGATCGGTTGGACAAAGGGAAAATCTCCGAAATCGACTTGGAAAGAGAGGAAGAATGTGGTATTTGCATGGAGCTAAACAGCAAGGTTGTCTTGCCTAGTTGCAATCATTCAATGTGCATGAAGTGTTACAGGAGTTGGTAAGCTTTCTGCTGTTTTCATCTGCTATCAGTTTCTAATTGAGTACTAGTCCTTTCTTTGCAAAAAAGGGTTGGATTATTTGTGGGTTATTTGAAGCTGATTTTTGCAACTTGTTATCTTTGTTTCAGGCGCACTCGATCTCAATCATGCCCCTTCTGCCGTGACAGTCTCAAAAGAGTCAACTCTGGAGATCTTTGGATATGTACCAGTAGTAGTGAAATCGTCGATTTGTCCTTCATTTCTAGGGAAAATTTGAAGAGGCTTTTCATGTTCATTAACAAGTTGCCTTTCATTGTTCCAGATCCAAAACTCATCTCTTATTATCTGAATCCCTATTAA

mRNA sequence

GCGAAAGCCAAGTGAGTCCCACTTCCCTTCTCTACGGCTGTTTTTCACTTCTCGAATTGCCGAATTTGCTTCGAAAGCCCTTTGGCGAAGAACTACACACAGAAAGGGGATTTCATAATTTCATATCTTGGATTTCTTTTAGAGGTGCGCCGTTGAAGACGAACGAGGTGTAAGGAATTTTCGCTTGCAGATTGCATAGATGAGGTAGGAGTTGTTGCTGCTGATACTGGAAGTGGTGGATGGGAGTTTCTAAGTGTGGAAATTAAGTCAAAATGCGGAAATCTTTCAAGGATTCCCTCAAGGCACTCGAGGCTGATATTCAGTTCGCCAATACCTTAATTGCTAATGCAATGTTTATTAGAATGTCAGTCATGTTGGTTTCGTTGCGTATAGAGTTTGAGGTGGTTTGTGAACTTTCGCATTGTCCAGCTAGTAGTTTTGTAAGATATCTTGACTTCTCAGGATTGGCTTCTGATTATCCCAAGGAATATGATGGAGCATGCCTACAAATGAGATTATCCTATAGCCCGGCTGCACAGTTCTTTCTCTTCTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGTCTGCTCAGAATTCTGATATATAAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGGGTGATATTTCCTTCTCTAGTGCAACTTCAAAGAGGAATTACCGACATAGAAGAGAGAAAACAGAGAGAAGTTTATGCTGCAAAATTCAAAAGAAAGGATCGGTTGGACAAAGGGAAAATCTCCGAAATCGACTTGGAAAGAGAGGAAGAATGTGGTATTTGCATGGAGCTAAACAGCAAGGTTGTCTTGCCTAGTTGCAATCATTCAATGTGCATGAAGTGTTACAGGAGTTGGCGCACTCGATCTCAATCATGCCCCTTCTGCCGTGACAGTCTCAAAAGAGTCAACTCTGGAGATCTTTGGATATGTACCAGTAGTAGTGAAATCGTCGATTTGTCCTTCATTTCTAGGGAAAATTTGAAGAGGCTTTTCATGTTCATTAACAAGTTGCCTTTCATTGTTCCAGATCCAAAACTCATCTCTTATTATCTGAATCCCTATTAA

Coding sequence (CDS)

ATGCGGAAATCTTTCAAGGATTCCCTCAAGGCACTCGAGGCTGATATTCAGTTCGCCAATACCTTAATTGCTAATGCAATGTTTATTAGAATGTCAGTCATGTTGGTTTCGTTGCGTATAGAGTTTGAGGTGGTTTGTGAACTTTCGCATTGTCCAGCTAGTAGTTTTGTAAGATATCTTGACTTCTCAGGATTGGCTTCTGATTATCCCAAGGAATATGATGGAGCATGCCTACAAATGAGATTATCCTATAGCCCGGCTGCACAGTTCTTTCTCTTCTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGTCTGCTCAGAATTCTGATATATAAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGGGTGATATTTCCTTCTCTAGTGCAACTTCAAAGAGGAATTACCGACATAGAAGAGAGAAAACAGAGAGAAGTTTATGCTGCAAAATTCAAAAGAAAGGATCGGTTGGACAAAGGGAAAATCTCCGAAATCGACTTGGAAAGAGAGGAAGAATGTGGTATTTGCATGGAGCTAAACAGCAAGGTTGTCTTGCCTAGTTGCAATCATTCAATGTGCATGAAGTGTTACAGGAGTTGGCGCACTCGATCTCAATCATGCCCCTTCTGCCGTGACAGTCTCAAAAGAGTCAACTCTGGAGATCTTTGGATATGTACCAGTAGTAGTGAAATCGTCGATTTGTCCTTCATTTCTAGGGAAAATTTGAAGAGGCTTTTCATGTTCATTAACAAGTTGCCTTTCATTGTTCCAGATCCAAAACTCATCTCTTATTATCTGAATCCCTATTAA

Protein sequence

MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYLDFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISEIDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY

Homology

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BLAST of Lsi03G022080.1 vs. ExPASy Swiss-Prot
Match: Q9M022 (E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 6.1e-96
Identity = 175/280 (62.50%), Postives = 205/280 (73.21%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LAS+YP+EYDG  +QMRLSYSPAA  FLF +QWTDCH AGALGLLRILIYKAY DG
Sbjct: 61  ----LASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAGALGLLRILIYKAYVDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMS+ ERK S++EFY V+FPSL+QL  GITD+EERKQ+E+   ++++KDR DKGK+SE
Sbjct: 121 KTTMSLHERKTSIREFYDVLFPSLLQLHGGITDVEERKQKEICDKRYRKKDRTDKGKMSE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGIC+E+ +KVVLP+CNHSMC+ CYR+WR RSQSCPFCR SLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICLEIRNKVVLPTCNHSMCINCYRNWRARSQSCPFCRGSLKRVNSGDLWI 237

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISY 281
            T S+EI DL  I +ENLKRL ++I+KLP +  DP L+ Y
Sbjct: 241 YTCSAEIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVPY 237

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BLAST of Lsi03G022080.1 vs. ExPASy Swiss-Prot
Match: Q803C1 (E3 ubiquitin-protein ligase rnf8 OS=Danio rerio OX=7955 GN=rnf8 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.3e-05
Identity = 34/105 (32.38%), Postives = 52/105 (49.52%), Query Frame = 0


Query: 127 QERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAK-FKRKDRLDKGKI----SEI 186
           ++ + +LKE   VI   L    +G  ++ + K +E+   K  K K +  K ++    +E+
Sbjct: 327 KQLEEALKEHRKVI-EELKHAWQGFKEVLQAKDKELEVTKEEKEKAKAQKEEVVTQMTEV 386

Query: 187 DLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFC 227
            LE E +C IC EL  + V  +C HS C  C   WR R   CP C
Sbjct: 387 -LESELQCSICSELFIEAVTLNCAHSFCQHCISEWRNRKDKCPMC 429

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BLAST of Lsi03G022080.1 vs. ExPASy Swiss-Prot
Match: Q9UHC7 (E3 ubiquitin-protein ligase makorin-1 OS=Homo sapiens OX=9606 GN=MKRN1 PE=1 SV=3)

HSP 1 Score: 48.1 bits (113), Expect = 1.9e-04
Identity = 25/76 (32.89%), Postives = 36/76 (47.37%), Query Frame = 0


Query: 181 IDLEREEECGICMELNSKVVLPS---------CNHSMCMKCYRSWRTRSQ-------SCP 240
           +   ++  CGICME+  +   PS         CNH+ C+KC R WR+  Q       SCP
Sbjct: 273 VQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCP 332

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BLAST of Lsi03G022080.1 vs. ExPASy Swiss-Prot
Match: Q9TT91 (E3 ubiquitin-protein ligase makorin-1 OS=Macropus eugenii OX=9315 GN=MKRN1 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.9e-04
Identity = 25/76 (32.89%), Postives = 36/76 (47.37%), Query Frame = 0


Query: 181 IDLEREEECGICMELNSKVVLPS---------CNHSMCMKCYRSWRTRSQ-------SCP 240
           +   ++  CGICME+  +   PS         CNH+ C+KC R WR+  Q       SCP
Sbjct: 269 VQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCP 328

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BLAST of Lsi03G022080.1 vs. ExPASy Swiss-Prot
Match: Q9QXP6 (E3 ubiquitin-protein ligase makorin-1 OS=Mus musculus OX=10090 GN=Mkrn1 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.9e-04
Identity = 25/68 (36.76%), Postives = 33/68 (48.53%), Query Frame = 0


Query: 189 CGICMELNSKVVLPS---------CNHSMCMKCYRSWRTRSQ-------SCPFCRDSLKR 241
           CGICME+  +   PS         CNH+ C+KC R WR+  Q       SCP CR +   
Sbjct: 281 CGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNF 340

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BLAST of Lsi03G022080.1 vs. ExPASy TrEMBL
Match: A0A0A0LA78 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731740 PE=4 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.9e-124
Identity = 234/285 (82.11%), Postives = 239/285 (83.86%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLVQLQ+GITDIEERKQREVYAAKFKR DRL+KGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVYAAKFKRTDRLNKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELN+KVVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDLSFISR+NLKRLFMFINKLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLNHY 242

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BLAST of Lsi03G022080.1 vs. ExPASy TrEMBL
Match: A0A5D3DMZ7 (E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00850 PE=4 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 2.1e-123
Identity = 232/285 (81.40%), Postives = 238/285 (83.51%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAK++R D L+KGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKYRRTDPLNKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           ID+EREEECGICMELN+KVVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDMEREEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDLSFISRENLKRLFMFINKLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLNHY 242

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BLAST of Lsi03G022080.1 vs. ExPASy TrEMBL
Match: A0A1S3B5N9 (uncharacterized protein LOC103486301 OS=Cucumis melo OX=3656 GN=LOC103486301 PE=4 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 2.1e-123
Identity = 232/285 (81.40%), Postives = 238/285 (83.51%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAK++R D L+KGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKYRRTDPLNKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           ID+EREEECGICMELN+KVVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDMEREEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDLSFISRENLKRLFMFINKLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080.1 vs. ExPASy TrEMBL
Match: A0A6J1CVC0 (uncharacterized protein LOC111015127 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015127 PE=4 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.4e-119
Identity = 226/285 (79.30%), Postives = 234/285 (82.11%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYP+EYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVYAAK+KRKD+LD+GKI+E
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELN  VVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
            T SSEIVDLSFISRENLKRLFMFI+KLP IVPDPKLISYYLN Y
Sbjct: 241 YTGSSEIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

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BLAST of Lsi03G022080.1 vs. ExPASy TrEMBL
Match: A0A6J1F2V6 (LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441644 PE=4 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 3.8e-117
Identity = 222/285 (77.89%), Postives = 230/285 (80.70%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEY GACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYGGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLV LQRG  DIE+R+ RE YAAK+KRKD+LDKGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVLLQRGTNDIEDRQHREAYAAKYKRKDQLDKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELNSKVVLP+C HSMCMKCYRSWR RSQSCPFCRDSL+RVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNSKVVLPTCCHSMCMKCYRSWRARSQSCPFCRDSLRRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSS+EIVDL FIS ENLKRLFMFI+KLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSTEIVDLPFISSENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

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BLAST of Lsi03G022080.1 vs. NCBI nr
Match: XP_038904042.1 (E3 ubiquitin-protein ligase AIRP2 isoform X1 [Benincasa hispida])

HSP 1 Score: 456.1 bits (1172), Expect = 2.3e-124
Identity = 236/285 (82.81%), Postives = 237/285 (83.16%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEY GACLQMRLSYSPAAQFFLFFVQWTDCH AGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYGGACLQMRLSYSPAAQFFLFFVQWTDCHFAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWR RSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRARSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDLSFISRENLKRLFMFI+KLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080.1 vs. NCBI nr
Match: XP_011651863.1 (E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >XP_031738614.1 E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >KGN58763.1 hypothetical protein Csa_000946 [Cucumis sativus])

HSP 1 Score: 455.3 bits (1170), Expect = 3.9e-124
Identity = 234/285 (82.11%), Postives = 239/285 (83.86%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLVQLQ+GITDIEERKQREVYAAKFKR DRL+KGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVYAAKFKRTDRLNKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELN+KVVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDLSFISR+NLKRLFMFINKLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080.1 vs. NCBI nr
Match: XP_008442427.1 (PREDICTED: uncharacterized protein LOC103486301 [Cucumis melo] >KAA0044165.1 E3 ubiquitin-protein ligase RNF8-B [Cucumis melo var. makuwa] >TYK24968.1 E3 ubiquitin-protein ligase RNF8-B [Cucumis melo var. makuwa])

HSP 1 Score: 451.8 bits (1161), Expect = 4.3e-123
Identity = 232/285 (81.40%), Postives = 238/285 (83.51%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAK++R D L+KGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKYRRTDPLNKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           ID+EREEECGICMELN+KVVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDMEREEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDLSFISRENLKRLFMFINKLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080.1 vs. NCBI nr
Match: XP_022145744.1 (uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145745.1 uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145746.1 uncharacterized protein LOC111015127 isoform X1 [Momordica charantia])

HSP 1 Score: 439.1 bits (1128), Expect = 2.9e-119
Identity = 226/285 (79.30%), Postives = 234/285 (82.11%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYP+EYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVYAAK+KRKD+LD+GKI+E
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELN  VVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
            T SSEIVDLSFISRENLKRLFMFI+KLP IVPDPKLISYYLN Y
Sbjct: 241 YTGSSEIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080.1 vs. NCBI nr
Match: XP_023527445.1 (LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 432.2 bits (1110), Expect = 3.5e-117
Identity = 223/285 (78.25%), Postives = 230/285 (80.70%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LASDYPKEY GACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG
Sbjct: 61  ----LASDYPKEYGGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMSIQERKASLKEFYGVIFPSLV LQRG  DIE+R+ RE YAAK+KRKD+LDKGKISE
Sbjct: 121 KTTMSIQERKASLKEFYGVIFPSLVLLQRGTNDIEDRQHREAYAAKYKRKDQLDKGKISE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGICMELNSKVVLP+C HSMCMKCYRSWR RSQSCPFCRDSL+RVNSGDLWI
Sbjct: 181 IDLEREEECGICMELNSKVVLPTCCHSMCMKCYRSWRARSQSCPFCRDSLRRVNSGDLWI 240

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           CTSSSEIVDL FIS ENLKRLFMFI+KLP IVPDPKLISYYLN Y
Sbjct: 241 CTSSSEIVDLPFISSENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Lsi03G022080.1 vs. TAIR 10
Match: AT5G01520.1 (RING/U-box superfamily protein )

HSP 1 Score: 352.1 bits (902), Expect = 4.3e-97
Identity = 175/280 (62.50%), Postives = 205/280 (73.21%), Query Frame = 0


Query: 1   MRKSFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYL 60
           MRKSFKDSLKALEADIQFANT                                       
Sbjct: 1   MRKSFKDSLKALEADIQFANT--------------------------------------- 60

Query: 61  DFSGLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDG 120
               LAS+YP+EYDG  +QMRLSYSPAA  FLF +QWTDCH AGALGLLRILIYKAY DG
Sbjct: 61  ----LASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAGALGLLRILIYKAYVDG 120

Query: 121 KTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISE 180
           KTTMS+ ERK S++EFY V+FPSL+QL  GITD+EERKQ+E+   ++++KDR DKGK+SE
Sbjct: 121 KTTMSLHERKTSIREFYDVLFPSLLQLHGGITDVEERKQKEICDKRYRKKDRTDKGKMSE 180

Query: 181 IDLEREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI 240
           IDLEREEECGIC+E+ +KVVLP+CNHSMC+ CYR+WR RSQSCPFCR SLKRVNSGDLWI
Sbjct: 181 IDLEREEECGICLEIRNKVVLPTCNHSMCINCYRNWRARSQSCPFCRGSLKRVNSGDLWI 237

Query: 241 CTSSSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISY 281
            T S+EI DL  I +ENLKRL ++I+KLP +  DP L+ Y
Sbjct: 241 YTCSAEIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVPY 237

[Close]

BLAST of Lsi03G022080.1 vs. TAIR 10
Match: AT3G47160.1 (RING/U-box superfamily protein )

HSP 1 Score: 322.0 bits (824), Expect = 4.8e-88
Identity = 162/282 (57.45%), Postives = 196/282 (69.50%), Query Frame = 0


Query: 4   SFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYLDFS 63
           SFKDSLKALEADIQ ANT                                          
Sbjct: 5   SFKDSLKALEADIQHANT------------------------------------------ 64

Query: 64  GLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTT 123
            +A DYP+E DGA +QMRLSY+PAAQF LF VQWTDCHLAG LGLLR+LIY  Y DGKTT
Sbjct: 65  -VALDYPREKDGARVQMRLSYTPAAQFLLFLVQWTDCHLAGTLGLLRVLIYMTYADGKTT 124

Query: 124 MSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISEIDL 183
           MS+ ERK S+K+FY VIFPSL+QL+RGITD+++RKQ+EV   +++ KD  +K K+SEID+
Sbjct: 125 MSVYERKTSIKDFYAVIFPSLLQLERGITDLDDRKQKEVCKIRYRNKDETEKVKLSEIDI 184

Query: 184 EREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTS 243
           EREEECGICME+N+ VVLP+C HS+C+KCYR W  RS+SCPFCRDSLKRVNSGDLW+   
Sbjct: 185 EREEECGICMEMNNMVVLPNCTHSLCIKCYRDWHGRSESCPFCRDSLKRVNSGDLWMLME 240

Query: 244 SSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
            S+ V++  I REN KRLF++I KLP +VPD    S   +PY
Sbjct: 245 KSDTVNMYTIERENKKRLFVYIEKLPLVVPDQVFAS---SPY 240

[Close]

BLAST of Lsi03G022080.1 vs. TAIR 10
Match: AT5G58787.1 (RING/U-box superfamily protein )

HSP 1 Score: 312.8 bits (800), Expect = 2.9e-85
Identity = 163/282 (57.80%), Postives = 196/282 (69.50%), Query Frame = 0


Query: 4   SFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYLDFS 63
           SFKDSLKALEADIQ ANT                                          
Sbjct: 5   SFKDSLKALEADIQHANT------------------------------------------ 64

Query: 64  GLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTT 123
            LA DYP+E DGA +QMRLSYSP AQFFLF VQWTDC LAG LGLLR+LIY  Y DGKTT
Sbjct: 65  -LALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCKLAGFLGLLRVLIYMTYADGKTT 124

Query: 124 MSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISEIDL 183
           MS+ ERKAS++EF  VI PSL QLQRG+TDI++ KQ+EV   ++++K   D+ ++SEI++
Sbjct: 125 MSVYERKASIREFQAVILPSLSQLQRGVTDIDDSKQKEVCKMRYRKK---DESEMSEIEI 184

Query: 184 EREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTS 243
           EREEECGICME+NSKVVLP+C HS+C+KCYR WR RSQSCPFCRDSLKRV+SGDLW+   
Sbjct: 185 EREEECGICMEMNSKVVLPNCTHSLCIKCYRDWRGRSQSCPFCRDSLKRVDSGDLWMFLD 237

Query: 244 SSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
            ++ V+L+ I+REN KRLFM+I KLP +VPD     Y  +PY
Sbjct: 245 QNDTVNLTAIARENQKRLFMYIEKLPLVVPDQ---VYASSPY 237

[Close]

BLAST of Lsi03G022080.1 vs. TAIR 10
Match: AT3G47160.2 (RING/U-box superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 6.5e-85
Identity = 145/220 (65.91%), Postives = 178/220 (80.91%), Query Frame = 0


Query: 66  ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 125
           A DYP+E DGA +QMRLSY+PAAQF LF VQWTDCHLAG LGLLR+LIY  Y DGKTTMS
Sbjct: 36  ALDYPREKDGARVQMRLSYTPAAQFLLFLVQWTDCHLAGTLGLLRVLIYMTYADGKTTMS 95

Query: 126 IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISEIDLER 185
           + ERK S+K+FY VIFPSL+QL+RGITD+++RKQ+EV   +++ KD  +K K+SEID+ER
Sbjct: 96  VYERKTSIKDFYAVIFPSLLQLERGITDLDDRKQKEVCKIRYRNKDETEKVKLSEIDIER 155

Query: 186 EEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 245
           EEECGICME+N+ VVLP+C HS+C+KCYR W  RS+SCPFCRDSLKRVNSGDLW+    S
Sbjct: 156 EEECGICMEMNNMVVLPNCTHSLCIKCYRDWHGRSESCPFCRDSLKRVNSGDLWMLMEKS 215

Query: 246 EIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
           + V++  I REN KRLF++I KLP +VPD    S   +PY
Sbjct: 216 DTVNMYTIERENKKRLFVYIEKLPLVVPDQVFAS---SPY 252

HSP 2 Score: 41.2 bits (95), Expect = 1.6e-03
Identity = 26/60 (43.33%), Postives = 37/60 (61.67%), Query Frame = 0


Query: 4  SFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFE---VVCELSHCPASSFVRYL 61
          SFKDSLKALEADIQ ANT+++N +   +    +    E +   V   LS+ PA+ F+ +L
Sbjct: 5  SFKDSLKALEADIQHANTVLSNELGDEVIFRALDYPREKDGARVQMRLSYTPAAQFLLFL 64

[Close]

BLAST of Lsi03G022080.1 vs. TAIR 10
Match: AT5G58787.2 (RING/U-box superfamily protein )

HSP 1 Score: 281.6 bits (719), Expect = 7.2e-76
Identity = 152/282 (53.90%), Postives = 184/282 (65.25%), Query Frame = 0


Query: 4   SFKDSLKALEADIQFANTLIANAMFIRMSVMLVSLRIEFEVVCELSHCPASSFVRYLDFS 63
           SFKDSLKALEADIQ ANT                                          
Sbjct: 5   SFKDSLKALEADIQHANT------------------------------------------ 64

Query: 64  GLASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTT 123
            LA DYP+E DGA +QMRLSYSP AQFFLF VQWTDC LAG LGLLR+LIY  Y DGKTT
Sbjct: 65  -LALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCKLAGFLGLLRVLIYMTYADGKTT 124

Query: 124 MSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISEIDL 183
           MS+ ERKAS++EF                DI++ KQ+EV   ++++K   D+ ++SEI++
Sbjct: 125 MSVYERKASIREF---------------QDIDDSKQKEVCKMRYRKK---DESEMSEIEI 184

Query: 184 EREEECGICMELNSKVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTS 243
           EREEECGICME+NSKVVLP+C HS+C+KCYR WR RSQSCPFCRDSLKRV+SGDLW+   
Sbjct: 185 EREEECGICMEMNSKVVLPNCTHSLCIKCYRDWRGRSQSCPFCRDSLKRVDSGDLWMFLD 222

Query: 244 SSEIVDLSFISRENLKRLFMFINKLPFIVPDPKLISYYLNPY 286
            ++ V+L+ I+REN KRLFM+I KLP +VPD     Y  +PY
Sbjct: 245 QNDTVNLTAIARENQKRLFMYIEKLPLVVPDQ---VYASSPY 222

The following BLAST results are available for this feature:

BLAST of Lsi03G022080.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M022	6.1e-96	62.50	E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 ...	[more]
Q803C1	1.3e-05	32.38	E3 ubiquitin-protein ligase rnf8 OS=Danio rerio OX=7955 GN=rnf8 PE=2 SV=1	[more]
Q9UHC7	1.9e-04	32.89	E3 ubiquitin-protein ligase makorin-1 OS=Homo sapiens OX=9606 GN=MKRN1 PE=1 SV=3	[more]
Q9TT91	1.9e-04	32.89	E3 ubiquitin-protein ligase makorin-1 OS=Macropus eugenii OX=9315 GN=MKRN1 PE=2 ...	[more]
Q9QXP6	1.9e-04	36.76	E3 ubiquitin-protein ligase makorin-1 OS=Mus musculus OX=10090 GN=Mkrn1 PE=1 SV=...	[more]

BLAST of Lsi03G022080.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LA78	1.9e-124	82.11	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731740 P...	[more]
A0A5D3DMZ7	2.1e-123	81.40	E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B5N9	2.1e-123	81.40	uncharacterized protein LOC103486301 OS=Cucumis melo OX=3656 GN=LOC103486301 PE=...	[more]
A0A6J1CVC0	1.4e-119	79.30	uncharacterized protein LOC111015127 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1F2V6	3.8e-117	77.89	LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 OS=Cuc...	[more]

BLAST of Lsi03G022080.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904042.1	2.3e-124	82.81	E3 ubiquitin-protein ligase AIRP2 isoform X1 [Benincasa hispida]	[more]
XP_011651863.1	3.9e-124	82.11	E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >XP_031738614.1 E3 ubiquitin...	[more]
XP_008442427.1	4.3e-123	81.40	PREDICTED: uncharacterized protein LOC103486301 [Cucumis melo] >KAA0044165.1 E3 ...	[more]
XP_022145744.1	2.9e-119	79.30	uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145...	[more]
XP_023527445.1	3.5e-117	78.25	LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 [Cucur...	[more]

BLAST of Lsi03G022080.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01520.1	4.3e-97	62.50	RING/U-box superfamily protein	[more]
AT3G47160.1	4.8e-88	57.45	RING/U-box superfamily protein	[more]
AT5G58787.1	2.9e-85	57.80	RING/U-box superfamily protein	[more]
AT3G47160.2	6.5e-85	65.91	RING/U-box superfamily protein	[more]
AT5G58787.2	7.2e-76	53.90	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 189..226 e-value: 2.2E-6 score: 37.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 189..227 score: 12.194218
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 186..232 e-value: 1.3E-9 score: 37.7
None	No IPR available	PANTHER	PTHR15315:SF31	E3 UBIQUITIN-PROTEIN LIGASE AIRP2	coord: 1..25 coord: 64..281
None	No IPR available	PANTHER	PTHR15315	RING FINGER PROTEIN 41, 151	coord: 1..25 coord: 64..281
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 175..232
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 164..235 e-value: 6.3E-15 score: 56.3
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 204..213

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi03G022080	Lsi03G022080	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi03G022080.1.exon.9	Lsi03G022080.1.exon.9	exon
Lsi03G022080.1.exon.8	Lsi03G022080.1.exon.8	exon
Lsi03G022080.1.exon.7	Lsi03G022080.1.exon.7	exon
Lsi03G022080.1.exon.6	Lsi03G022080.1.exon.6	exon
Lsi03G022080.1.exon.5	Lsi03G022080.1.exon.5	exon
Lsi03G022080.1.exon.4	Lsi03G022080.1.exon.4	exon
Lsi03G022080.1.exon.3	Lsi03G022080.1.exon.3	exon
Lsi03G022080.1.exon.2	Lsi03G022080.1.exon.2	exon
Lsi03G022080.1.exon.1	Lsi03G022080.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi03G022080.1.CDS.8	Lsi03G022080.1.CDS.8	CDS
Lsi03G022080.1.CDS.7	Lsi03G022080.1.CDS.7	CDS
Lsi03G022080.1.CDS.6	Lsi03G022080.1.CDS.6	CDS
Lsi03G022080.1.CDS.5	Lsi03G022080.1.CDS.5	CDS
Lsi03G022080.1.CDS.4	Lsi03G022080.1.CDS.4	CDS
Lsi03G022080.1.CDS.3	Lsi03G022080.1.CDS.3	CDS
Lsi03G022080.1.CDS.2	Lsi03G022080.1.CDS.2	CDS
Lsi03G022080.1.CDS.1	Lsi03G022080.1.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi03G022080.1.five_prime_UTR.2	Lsi03G022080.1.five_prime_UTR.2	five_prime_UTR
Lsi03G022080.1.five_prime_UTR.1	Lsi03G022080.1.five_prime_UTR.1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi03G022080.1	Lsi03G022080.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009789	positive regulation of abscisic acid-activated signaling pathway
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0009651	response to salt stress
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005829	cytosol
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity