Lcy13g008730.1 (mRNA) Sponge gourd (P93075) v1

Overview


Name	Lcy13g008730.1
Type	mRNA
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	Chr13: 8546882 .. 8549900 (+)
Sequence length	1684
RNA-Seq Expression	Lcy13g008730.1
Synteny	Lcy13g008730.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCAGAATGCGCTCCCAGTGTTCCTCTCTCCGCCGCCGTCCGACCATGTCCGCCCCCGCCGCCGCGGTGCTCCTCCTTCTCCTCCAAATCTCCACCGCCGCCGTCGCCATTGGCATCAACTATGGCACTCTGGCGAACAACCTCCGGCCGCCGGCGGAGGTGGTGAACTTCATCAGAACAAAAACCAAAATCGACCGCGTGAAGCTCTTCGATACGAATCCGGAGATCCTACGGGCTTTCGCCGGCACCGGAATTCTGGTGACGGTGACTGTCGGCAATGGCGAGATCCCGGCACTGTCGGATCTGGCCAACGCTCGGCAATGGGTCGCTGCCCATATCGCACCGTTTCACCCTCAGACGCGGATTCATTACATTGCTGTGGGAAATGAAATCATGCACTCCAACGATAAGCCTCTGATCGCGCGTCTCGTTCCCGCCATGAGAGCTATCCACCGAGCTCTGGTTCTCGCCGGAATTACGGACATTAAGGTAATGACGTTGATATTTTTTAATTTTTTATTTATTTATTTCGCCATGGAAAACCGTTTTAAGCTGTTGGGTTGTAATGATTCTTTAGAGTGATCTTAAAATTAAATTAAGGATGGATTTTAAATGAGTTTTGTTATTTGAGTGATTTCTAATAATCATTTATATATATTTTTCAAAAAAAAAAAAAAAAAAAATTAGTCTATAGTATAAATTTGGTATCATAAAAATAGTGCTACTTTTCGCTTTCTTTTTTTCAAACACAACACATTATATTTGAATTTTAAATTGATTTCAAACTTACAAGATATAGTTTGTTTTCAATTTTCATAAAATGTCACATAATGCTCAACTGGCATGAAACCGATAATCACGAGGTTTGTGGTTCGAATCCCTCATCTCATATATTGTCAAAAATATATATATATATATATATATTATACATTAACATGAAAACTACTCAAGAAGATTTGTAAATAATTTTCAAAATCTCTAACGACTTCGTTTTGTCATCCAATCAGGTCACATCTCCACATTCATTGGGCATTCTCTCGATCTCCGAGCCACCAAGTATGGGTCGGTTTAGGCGAGGGTATGATCGGACCGTCTTCACACCAATGCTCCAATTCCTACGAGAAACCAAGGCCCCGTTCATGGTCAACCCATACCCCTACTTTGCCTTCTCACCAAAGATGCCCAATTACGCCTTGTTTCGACCCAACCGCGGCATTTACGACAAGTTCACCAAAATCAGATACACCAATATGTTCGACGGCATGATGGACGCCCTCCACTCCGCCATGAAGGCCCTCGGCTTCGCCGACGTCGATCTTGTCATCGGCGAGACTGGTTGGCCCACGAGTTGCGATGGCTACTTCGCTTGTAATCCTCAAAATGCTGCTACTTATAACAGGAATTTGATTTGGCATGTGAACTCTGGTAAGGGGACGCCGCTGATGCCGAACCGAAAATTTGAGACTTACTTGTTTGCTTTGTTTAACGAGAATCTTAAACCCGGTCCGGATGCCGAGCGAAATTGGGGTTTGTTTCAGCCCGACTTTACTCCGGTTTATGACCTTCCCAACATCATGCGTGGTGGACGAGTTCGTTTCTTTTTCTCTACCTTCTCATGATTTTTTTTTTTTGCTTGCTTTTAAAATGTGTCTATTTTTTTTTTGAAACAGTGTCTATTTTCTTTTTAAAATAACCATTTAAGATTTTTTTTTCCTCTCTCAGAATGTTTTATTTTAGTTTTTAATTTTTTTCCAAATTAATAATTTAGTTTTTTACTATTATTTTGCCACTTAGTTATCTAGATTTGAAGGATTGTGTAGAAAAATAATAATTAGAGTTTTCTTTTTTATTTGCCTTGTTCTGAATTTTAAAATGGAAAGATTTTATATATGTGTGTATATATATATATAAATCATCTATCTATCTAATTATTAAAATTAAAAATAAAAAATATGACAAAAAGAATAATTTCTTTTAAAAGTGTAGAGATTTATTTTTAGTCCTAAAATTAATTTAGAGATCAAAATATAAATTGACCAAAACAAAAAAGCTAATGATTTATTTGTTTGCCTTCCTTCTATTTTCAAAAAAAAAAAAAAAAAAATCCCATTTTTTTTTTAAATAAAAAGAAAAAGATTTGAAAATAACAAAAAAAGAAAAATATTACACTCACTTCATATGTGTATTATATTACATCTCAAATTCAAGGATTTAAAACCATTTCCTAAATTATTTTTAAATCATTTCCTAAGCATCTTATTCGTCACATCTTCCCCATAACAAACCAACTAATGATTTGGGATTTTACTTTCATTTACAGAACGGCCGTCGTGGAGGTGGCCAAAGGAGGCCGACGCCGGTGGCGCCGTCGGGGAAGAAATGGTGCGTTCCGAGATCGGACGCGGCCATTGAAGCCTTACAGAAGAACATCGACTACGTCTGCAGCTCGAACATCAATTGCCGTCCGATTCAAGCCGGCGGCGCCTGTTTCCTTCCCAACGACATCCGATCGCACGCCTCTTACGCCATGAATTCATATTATCAGTCCTCCGGCCGCAACGATTTCAACTGTGATTTCGGTCACACCGGCGTTCTAACCACCGTCGATCCAAGTAATTGCTCTTTCTCTCATTTCCTGAATTGAATGAATTTGATTCCAGATTTTGTGATGTGGCTGACTGGCGGCGATCTCGTGCAGGTCACGGCGGATGTGCATACGCTTCTTGAGAGATCGAGCGGCGGAAGCGGAGGAGGGATTTTGGTTGCCTGCGGGCGGGTGCAGGAGAGAAAACTGTGAGACCACATAAAGCTTGCTAATCAATAGAGCCAGCTGCAAATACTTTTTCTTTTTTGCTTTTTATTTTTCTTTCATATAATGGTTTATTTTCCATTTGTTTGTAATGTAAGTAAACTGGATTCTTCTAGAAACATCTCGCTCTCATTTTTACAAAAGTGAGATTTGGAGATTGTAAAGGATTACATTAATTATTCAAACAATATTGTGTCCCTTCTTTGTTTA

mRNA sequence

TTCAGAATGCGCTCCCAGTGTTCCTCTCTCCGCCGCCGTCCGACCATGTCCGCCCCCGCCGCCGCGGTGCTCCTCCTTCTCCTCCAAATCTCCACCGCCGCCGTCGCCATTGGCATCAACTATGGCACTCTGGCGAACAACCTCCGGCCGCCGGCGGAGGTGGTGAACTTCATCAGAACAAAAACCAAAATCGACCGCGTGAAGCTCTTCGATACGAATCCGGAGATCCTACGGGCTTTCGCCGGCACCGGAATTCTGGTGACGGTGACTGTCGGCAATGGCGAGATCCCGGCACTGTCGGATCTGGCCAACGCTCGGCAATGGGTCGCTGCCCATATCGCACCGTTTCACCCTCAGACGCGGATTCATTACATTGCTGTGGGAAATGAAATCATGCACTCCAACGATAAGCCTCTGATCGCGCGTCTCGTTCCCGCCATGAGAGCTATCCACCGAGCTCTGGTTCTCGCCGGAATTACGGACATTAAGGTCACATCTCCACATTCATTGGGCATTCTCTCGATCTCCGAGCCACCAAGTATGGGTCGGTTTAGGCGAGGGTATGATCGGACCGTCTTCACACCAATGCTCCAATTCCTACGAGAAACCAAGGCCCCGTTCATGGTCAACCCATACCCCTACTTTGCCTTCTCACCAAAGATGCCCAATTACGCCTTGTTTCGACCCAACCGCGGCATTTACGACAAGTTCACCAAAATCAGATACACCAATATGTTCGACGGCATGATGGACGCCCTCCACTCCGCCATGAAGGCCCTCGGCTTCGCCGACGTCGATCTTGTCATCGGCGAGACTGGTTGGCCCACGAGTTGCGATGGCTACTTCGCTTGTAATCCTCAAAATGCTGCTACTTATAACAGGAATTTGATTTGGCATGTGAACTCTGGTAAGGGGACGCCGCTGATGCCGAACCGAAAATTTGAGACTTACTTGTTTGCTTTGTTTAACGAGAATCTTAAACCCGGTCCGGATGCCGAGCGAAATTGGGGTTTGTTTCAGCCCGACTTTACTCCGGTTTATGACCTTCCCAACATCATGCGTGGTGGACGAAACGGCCGTCGTGGAGGTGGCCAAAGGAGGCCGACGCCGGTGGCGCCGTCGGGGAAGAAATGGTGCGTTCCGAGATCGGACGCGGCCATTGAAGCCTTACAGAAGAACATCGACTACGTCTGCAGCTCGAACATCAATTGCCGTCCGATTCAAGCCGGCGGCGCCTGTTTCCTTCCCAACGACATCCGATCGCACGCCTCTTACGCCATGAATTCATATTATCAGTCCTCCGGCCGCAACGATTTCAACTGTGATTTCGGTCACACCGGCGTTCTAACCACCGTCGATCCAAGTCACGGCGGATGTGCATACGCTTCTTGAGAGATCGAGCGGCGGAAGCGGAGGAGGGATTTTGGTTGCCTGCGGGCGGGTGCAGGAGAGAAAACTGTGAGACCACATAAAGCTTGCTAATCAATAGAGCCAGCTGCAAATACTTTTTCTTTTTTGCTTTTTATTTTTCTTTCATATAATGGTTTATTTTCCATTTGTTTGTAATGTAAGTAAACTGGATTCTTCTAGAAACATCTCGCTCTCATTTTTACAAAAGTGAGATTTGGAGATTGTAAAGGATTACATTAATTATTCAAACAATATTGTGTCCCTTCTTTGTTTA

Coding sequence (CDS)

ATGCGCTCCCAGTGTTCCTCTCTCCGCCGCCGTCCGACCATGTCCGCCCCCGCCGCCGCGGTGCTCCTCCTTCTCCTCCAAATCTCCACCGCCGCCGTCGCCATTGGCATCAACTATGGCACTCTGGCGAACAACCTCCGGCCGCCGGCGGAGGTGGTGAACTTCATCAGAACAAAAACCAAAATCGACCGCGTGAAGCTCTTCGATACGAATCCGGAGATCCTACGGGCTTTCGCCGGCACCGGAATTCTGGTGACGGTGACTGTCGGCAATGGCGAGATCCCGGCACTGTCGGATCTGGCCAACGCTCGGCAATGGGTCGCTGCCCATATCGCACCGTTTCACCCTCAGACGCGGATTCATTACATTGCTGTGGGAAATGAAATCATGCACTCCAACGATAAGCCTCTGATCGCGCGTCTCGTTCCCGCCATGAGAGCTATCCACCGAGCTCTGGTTCTCGCCGGAATTACGGACATTAAGGTCACATCTCCACATTCATTGGGCATTCTCTCGATCTCCGAGCCACCAAGTATGGGTCGGTTTAGGCGAGGGTATGATCGGACCGTCTTCACACCAATGCTCCAATTCCTACGAGAAACCAAGGCCCCGTTCATGGTCAACCCATACCCCTACTTTGCCTTCTCACCAAAGATGCCCAATTACGCCTTGTTTCGACCCAACCGCGGCATTTACGACAAGTTCACCAAAATCAGATACACCAATATGTTCGACGGCATGATGGACGCCCTCCACTCCGCCATGAAGGCCCTCGGCTTCGCCGACGTCGATCTTGTCATCGGCGAGACTGGTTGGCCCACGAGTTGCGATGGCTACTTCGCTTGTAATCCTCAAAATGCTGCTACTTATAACAGGAATTTGATTTGGCATGTGAACTCTGGTAAGGGGACGCCGCTGATGCCGAACCGAAAATTTGAGACTTACTTGTTTGCTTTGTTTAACGAGAATCTTAAACCCGGTCCGGATGCCGAGCGAAATTGGGGTTTGTTTCAGCCCGACTTTACTCCGGTTTATGACCTTCCCAACATCATGCGTGGTGGACGAAACGGCCGTCGTGGAGGTGGCCAAAGGAGGCCGACGCCGGTGGCGCCGTCGGGGAAGAAATGGTGCGTTCCGAGATCGGACGCGGCCATTGAAGCCTTACAGAAGAACATCGACTACGTCTGCAGCTCGAACATCAATTGCCGTCCGATTCAAGCCGGCGGCGCCTGTTTCCTTCCCAACGACATCCGATCGCACGCCTCTTACGCCATGAATTCATATTATCAGTCCTCCGGCCGCAACGATTTCAACTGTGATTTCGGTCACACCGGCGTTCTAACCACCGTCGATCCAAGTCACGGCGGATGTGCATACGCTTCTTGA

Protein sequence

MRSQCSSLRRRPTMSAPAAAVLLLLLQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAFAGTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLIARLVPAMRAIHRALVLAGITDIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPMLQFLRETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMDALHSAMKALGFADVDLVIGETGWPTSCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFALFNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGGQRRPTPVAPSGKKWCVPRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSYYQSSGRNDFNCDFGHTGVLTTVDPSHGGCAYAS

Homology

[Close]

BLAST of Lcy13g008730.1 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 2.8e-127
Identity = 231/459 (50.33%), Postives = 305/459 (66.45%), Query Frame = 0


Query: 21  VLLLLL-------QISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPE 80
           +LL+LL       + +T ++ IG+NYG  A+NL  P  V  F+ TKT IDRVKLFD NP 
Sbjct: 5   ILLMLLAAGAAGAESATPSLHIGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANPT 64

Query: 81  ILRAFAGTGILVTVTVGNGEIPALSDLAN----ARQWVAAHIAPFHPQTRIHYIAVGNEI 140
            + AFAGT I + V++ N  +PAL+D A     AR W+ A+++P+ P T +  +  GNEI
Sbjct: 65  FISAFAGTPISLAVSLPNSALPALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNEI 124

Query: 141 MHSNDKPLIARLVPAMRAIHRALVLAGITDIKVTSPHSLGILSISEP-PSMGRFRRGYDR 200
           + S D  LI  L+PAMR + +AL   G+T ++VT+PH LGIL+ S+  PS   FR GY+ 
Sbjct: 125 LLSTDTNLILSLLPAMRRLAQALKAEGLTGVRVTTPHYLGILAPSDGIPSNASFRAGYNT 184

Query: 201 TVFTPMLQFLRETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMM 260
            +F  MLQF R+T +PFMVNPYPYF++ P+  NYALFRPN GIYD  TK+ YT+M D  M
Sbjct: 185 KLFPAMLQFHRDTGSPFMVNPYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLDAQM 244

Query: 261 DALHSAMKALGFADVDLVIGETGWPTSCD-GYFACNPQNAATYNRNLIWHVNSGKGTPLM 320
           DA+++AMK LG+ DVD+ +GE GWPT  + G      Q A  +N  +I   +SGKGTPLM
Sbjct: 245 DAIYTAMKKLGYGDVDIAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTPLM 304

Query: 321 PNRKFETYLFALFNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRN-----GRRGGG 380
           PNR FETYLF+LF+EN KPGP AER++GLF PDFTPVYDL  ++R G +           
Sbjct: 305 PNRTFETYLFSLFDENQKPGPIAERHFGLFNPDFTPVYDL-GLLRDGASVAPTPSPNPSP 364

Query: 381 QRRPTPVAPSGKKWCVPRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHAS 440
              P P    G KWCV +  A    LQ NI+Y C   ++C+PIQ+GGACF PN +++HAS
Sbjct: 365 NPSPKPAPSGGGKWCVAKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPNSLQAHAS 424

Query: 441 YAMNSYYQSSGRNDFNCDFGHTGVLTTVDPSHGGCAYAS 462
           Y MN+YYQ++G  D  CDF  TG++T+ DPS+GGC Y S
Sbjct: 425 YVMNAYYQANGHTDLACDFKGTGIVTSSDPSYGGCKYVS 461

[Close]

BLAST of Lcy13g008730.1 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 374.0 bits (959), Expect = 2.4e-102
Identity = 192/442 (43.44%), Postives = 280/442 (63.35%), Query Frame = 0


Query: 29  STAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAFAGTGILVTVT 88
           S A   IG+NYG +A+NL PP+E V  +++ T I +V+L+  +P I++A AGTG+ + + 
Sbjct: 20  SHAEPFIGVNYGQVADNLPPPSETVKLLQS-TSIQKVRLYGADPAIIKALAGTGVGIVIG 79

Query: 89  VGNGEIPAL-SDLANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLIARLVPAMRA 148
             NG++P+L SD   A QW+ +++ PF+P ++I  I VGNEI+ SND  L+ +L+PAM+ 
Sbjct: 80  AANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQN 139

Query: 149 IHRALVLAGI-TDIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPMLQFLRETKAPFM 208
           + +AL    +   IKV++ +S+ +L  S+PPS G F  GY +T    +LQFL +T +PF 
Sbjct: 140 VQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGY-QTGLKGILQFLSDTGSPFA 199

Query: 209 VNPYPYFAFS----PKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMDALHSAMKALGFAD 268
           +NPYP+FA+     P+   + LF PN G  D  T I+YTNMFD  +DA+HSA+K++GF  
Sbjct: 200 INPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEK 259

Query: 269 VDLVIGETGWPTSCD-GYFACNPQNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFALFN 328
           V++V+ ETGW +  D      +  NA  YN NLI H+ S  GTPLMP +  +TY+FAL++
Sbjct: 260 VEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYD 319

Query: 329 ENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGGQRRPTPVAPSGK----KWC 388
           ENLKPGP +ER +GLF+ D + VYD+            G  +   +   PSGK     WC
Sbjct: 320 ENLKPGPSSERAFGLFKTDLSMVYDV------------GLAKSSSSSQTPSGKVTSSGWC 379

Query: 389 VPRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSYYQSSGRNDF 448
           VP+  A  E LQ ++D+ C   I+C  IQ GGACF PN++ SHA+YAMN Y+Q S +   
Sbjct: 380 VPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPT 439

Query: 449 NCDFGHTGVLTTVDPSHGGCAY 460
           +CDF  T  +T+ +PS+  C Y
Sbjct: 440 DCDFSKTATVTSQNPSYNNCVY 447

[Close]

BLAST of Lcy13g008730.1 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 3.1e-97
Identity = 187/451 (41.46%), Postives = 278/451 (61.64%), Query Frame = 0


Query: 21  VLLLLLQISTAAVA---IGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRA 80
           ++ LLLQ   +A +   +G+NYG L++NL      VN +++ T I +V+LF   P +++A
Sbjct: 13  LVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKS-TTIQKVRLFGAEPAVIKA 72

Query: 81  FAGTGILVTVTVGNGEIPALSDLAN-ARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKP 140
           FA TG+ + +   NG+IP L+   N A Q+V +++  F+P + I  I VGNE++ S D+ 
Sbjct: 73  FANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSGDQK 132

Query: 141 LIARLVPAMRAIHRALVLAGI-TDIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPML 200
           LI++L+PAM+ +  AL  A +   +KV++ H++ +LS S PPS G F  G   T+   +L
Sbjct: 133 LISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGDTM-KALL 192

Query: 201 QFLRETKAPFMVNPYPYFAF----SPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMDAL 260
           QF     APFM++PYPYFA+    +P    + LF+PN G  D     +YTNMFD  +DA+
Sbjct: 193 QFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDAV 252

Query: 261 HSAMKALGFADVDLVIGETGWPTSCD-GYFACNPQNAATYNRNLIWHVNSGKGTPLMPNR 320
           HSA+ A+GF D+++V+ ETGWP   D      +  NA  Y  NLI H+ S  GTPLMP +
Sbjct: 253 HSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPGK 312

Query: 321 KFETYLFALFNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGGQRRPTPVA 380
             +TYLF+L++E+ K G  +E+ +GLF+PD +  YD+  +    +N +       PTP  
Sbjct: 313 SIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLL----KNTQNPTTPATPTPTP 372

Query: 381 PSGKKWCVPRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSYYQ 440
            +   WCVP+   + + L  NI+Y C   I+C PIQ GGACF PN +++HA+Y MN YYQ
Sbjct: 373 KAAGSWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQ 432

Query: 441 SSGRNDFNCDFGHTGVLTTVDPSHGGCAYAS 462
           S+GRN +NCDF  T  LT  +PS+G C + S
Sbjct: 433 SAGRNSWNCDFSQTATLTNTNPSYGACNFPS 457

[Close]

BLAST of Lcy13g008730.1 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.0e-80
Identity = 176/461 (38.18%), Postives = 253/461 (54.88%), Query Frame = 0


Query: 32  AVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAFAGTGILVTVTVGN 91
           A  IGI YG  A+NL  P  V   I+    I  V+++D N ++L+AFA TGI + + V N
Sbjct: 24  ASKIGICYGRNADNLPSPNRVSELIQ-HLNIKFVRIYDANIDVLKAFANTGIELMIGVPN 83

Query: 92  GEIPALSDL-ANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLIARLVPAMRAIHR 151
            ++ A +   +N   W++ +I P++P T+I  I+VG E+  + D      ++PAMR IH 
Sbjct: 84  ADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDN-ATGLVLPAMRNIHT 143

Query: 152 ALVLAGI-TDIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPMLQFLRETKAPFMVNP 211
           AL  +G+   IK++S HSL ILS S PPS   F + +      PML+FL E ++PFM++ 
Sbjct: 144 ALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHS-AFLKPMLEFLVENESPFMIDL 203

Query: 212 YPYFAF---SPKMP-NYALFRPNRGIYDKFTKIRYTNMFDGMMDALHSAMKALGFADVDL 271
           YPY+A+   + K+P  YALF  +  + D  T + Y+NMFD  +DA++ A+ A+ F  V +
Sbjct: 204 YPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKV 263

Query: 272 VIGETGWPT-SCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFALFNENL 331
           ++ E+GWP+       A  P+NA  YN NLI HV    GTP  P  + + YLF+LFNEN 
Sbjct: 264 MVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENR 323

Query: 332 KPGPDAERNWGLFQPDFTPVYDL-------------------------PNIMRGGRNGRR 391
           KPG ++ERNWG+F  + T VY L                           I+ G      
Sbjct: 324 KPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTI 383

Query: 392 GGGQRRPTPVAPSGKKWCVPRSDAAIEALQKNIDYVCS-SNINCRPIQAGGACFLPNDIR 451
           GGG    T      KKWC+  S A++  LQ  +D+ C   N++C  +Q    CF P+ + 
Sbjct: 384 GGGGGGGT------KKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVL 443

Query: 452 SHASYAMNSYYQSSGRNDFNCDFGHTGVLTTVDPSHGGCAY 460
           SHASYA N+YYQ SG +  +C F    V    DPS+G C Y
Sbjct: 444 SHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

[Close]

BLAST of Lcy13g008730.1 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 3.8e-79
Identity = 179/452 (39.60%), Postives = 250/452 (55.31%), Query Frame = 0


Query: 20  AVLLLLLQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAFA 79
           ++LLLLL        +G+ YG  A++L  P++VV  I+ +  I  V+++D N ++L+AF 
Sbjct: 12  SILLLLLDCCYGG-KVGVCYGRSADDLPTPSKVVQLIQ-QHNIKYVRIYDYNSQVLKAFG 71

Query: 80  GTGILVTVTVGNGEIPALS-DLANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLI 139
            T I + + V N ++ A S   +N   W+   + P++P T+I YI VG E   S D P I
Sbjct: 72  NTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAE---STDDPHI 131

Query: 140 ---ARLVPAMRAIHRALVLAGIT-DIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPM 199
              + +VPAM+ +  AL   G++  IKV++  SLGILS S PPS G F   Y      PM
Sbjct: 132 NASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSY-AYFLRPM 191

Query: 200 LQFLRETKAPFMVNPYPYFAFSPKMPN----YALFRPNRGIYDKFTKIRYTNMFDGMMDA 259
           L+FL E K+PFM++ YPY+A+     N    Y LF  +  + D  T + Y NMFD  +DA
Sbjct: 192 LEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDA 251

Query: 260 LHSAMKALGFADVDLVIGETGWPT--SCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMP 319
           L+ A+ AL F  + +++ ETGWPT  S     A +  NA TYN N+I HV + +GTP  P
Sbjct: 252 LYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKP 311

Query: 320 NRKFETYLFALFNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGGQRRPTP 379
                 Y+F+LFNEN K G D+ERNWGLF PD T VY L     G  NG         T 
Sbjct: 312 GEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLD--FTGKSNGFHSNSS--GTN 371

Query: 380 VAPSGKKWCVPRSDAAIEALQKNIDYVCS-SNINCRPIQAGGACFLPNDIRSHASYAMNS 439
            + S   WC+  S A+   L+  +D+ C   N++C  IQ    CF P+ + SHAS+  NS
Sbjct: 372 SSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNS 431

Query: 440 YYQSSGRNDFNCDFGHTGVLTTVDPSHGGCAY 460
           Y+Q +   D  C FG  GV    DPS+  C Y
Sbjct: 432 YFQQNRATDVACSFGGAGVKVNKDPSYDKCIY 453

[Close]

BLAST of Lcy13g008730.1 vs. ExPASy TrEMBL
Match: A0A6J1JYX6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111489139 PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 8.6e-228
Identity = 377/442 (85.29%), Postives = 414/442 (93.67%), Query Frame = 0


Query: 20  AVLLLLLQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAFA 79
           A LL+LLQISTAAVAIG+NYGTLANNLRPPAEVVNFI+TKTKIDRVK+FDTNP+ILRAFA
Sbjct: 11  AALLILLQISTAAVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAFA 70

Query: 80  GTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLIA 139
           GTGILVTVTVGNGEIPAL+DLA ARQWVAAHI PFHPQTRI+YIAVGNEI+H+NDKPLIA
Sbjct: 71  GTGILVTVTVGNGEIPALTDLATARQWVAAHIRPFHPQTRINYIAVGNEIIHTNDKPLIA 130

Query: 140 RLVPAMRAIHRALVLAGITDIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPMLQFLR 199
           RLVPAM+++HRALVLAGITDIKVTSPHSL ILSISEPPSMGRFRRGYDR +F PMLQFLR
Sbjct: 131 RLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRAIFGPMLQFLR 190

Query: 200 ETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMDALHSAMKALG 259
           ETKAPFMVNPYPYF +SP M NYALF PNRG+YDK+TKI YTNMFD MMDAL+SAMK +G
Sbjct: 191 ETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMDALYSAMKNIG 250

Query: 260 FADVDLVIGETGWPTSCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFAL 319
           ++DVDLVIGETGWP++CDGY AC+P NAAT++RNL+WHVNSGKGTPLMPNRKFETYLFAL
Sbjct: 251 YSDVDLVIGETGWPSNCDGYVACSPGNAATFHRNLVWHVNSGKGTPLMPNRKFETYLFAL 310

Query: 320 FNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGGQRRPTPVAPSGKKWCVP 379
           FNENLKPGP AERNWGLF PDFTPVYD+PNIM GGR+  RGG   RP PVAPSGKKWCVP
Sbjct: 311 FNENLKPGPTAERNWGLFHPDFTPVYDVPNIMIGGRH--RGGRGGRPKPVAPSGKKWCVP 370

Query: 380 RSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSYYQSSGRNDFNC 439
           +SDA+++ALQKNIDYVCSSNI+CRPIQAGGACFLPNDIR+HASYAMNSYYQ+SGRNDFNC
Sbjct: 371 KSDASVQALQKNIDYVCSSNIDCRPIQAGGACFLPNDIRAHASYAMNSYYQTSGRNDFNC 430

Query: 440 DFGHTGVLTTVDPSHGGCAYAS 462
           DF  TGV+TTV+PSHGGC Y S
Sbjct: 431 DFSLTGVITTVNPSHGGCVYIS 450

[Close]

BLAST of Lcy13g008730.1 vs. ExPASy TrEMBL
Match: A0A6J1GZM3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111458933 PE=3 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 9.5e-227
Identity = 377/443 (85.10%), Postives = 412/443 (93.00%), Query Frame = 0


Query: 20  AVLLLLLQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAFA 79
           A LL+LLQISTAAVAIG+NYGTL NNLRPPAEVVNFI+TKTKIDRVK+FDTNP+ILRAFA
Sbjct: 11  AALLILLQISTAAVAIGVNYGTLGNNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAFA 70

Query: 80  GTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLIA 139
           G+GILVTVTVGNGEIP L+DLA ARQWVAAHI PFHPQTRIHYIAVGNEI+H+NDKPLIA
Sbjct: 71  GSGILVTVTVGNGEIPGLTDLATARQWVAAHIRPFHPQTRIHYIAVGNEIIHTNDKPLIA 130

Query: 140 RLVPAMRAIHRALVLAGITDIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPMLQFLR 199
           RLVPAM+++HRALVLAGITDIKVTSPHSL ILSISEPPSMGRFRRGYDR +F PMLQFLR
Sbjct: 131 RLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRAIFAPMLQFLR 190

Query: 200 ETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMDALHSAMKALG 259
           ETKAPFMVNPYPYF +SP M NYALF PNRG+YDK+TKI YTNMFD MMDAL+SAMK +G
Sbjct: 191 ETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMDALYSAMKNIG 250

Query: 260 FADVDLVIGETGWPTSCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFAL 319
           ++DVDLVIGETGWPT+CD Y AC+P NAAT++RNL+WHVNSGKGTPLMPNRKFETYLFAL
Sbjct: 251 YSDVDLVIGETGWPTNCD-YVACSPNNAATFHRNLVWHVNSGKGTPLMPNRKFETYLFAL 310

Query: 320 FNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRN-GRRGGGQRRPTPVAPSGKKWCV 379
           FNENLKPGP AERNWGLF PDFTPVYD+PNIM GGR+ G RGGG   P PVAPSGKKWCV
Sbjct: 311 FNENLKPGPTAERNWGLFYPDFTPVYDVPNIMIGGRHRGGRGGG---PKPVAPSGKKWCV 370

Query: 380 PRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSYYQSSGRNDFN 439
           P+SDA+++ALQ NIDYVCSSNI+CRPIQAGGACFLPNDIRSHASYAMNSYYQ+SGRNDFN
Sbjct: 371 PKSDASVQALQNNIDYVCSSNIDCRPIQAGGACFLPNDIRSHASYAMNSYYQTSGRNDFN 430

Query: 440 CDFGHTGVLTTVDPSHGGCAYAS 462
           CDF HTGV+TTV+PSHGGC Y S
Sbjct: 431 CDFSHTGVITTVNPSHGGCVYIS 449

[Close]

BLAST of Lcy13g008730.1 vs. ExPASy TrEMBL
Match: A0A5D3BZM0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00090 PE=3 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 4.6e-221
Identity = 366/443 (82.62%), Postives = 405/443 (91.42%), Query Frame = 0


Query: 19  AAVLLLLLQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAF 78
           +A + LLLQIST   AIG+NYGTL NNLRPPAEVVNF++TKTKIDRVK+FDTNP+ILRAF
Sbjct: 10  SAAIFLLLQISTTVFAIGVNYGTLGNNLRPPAEVVNFLKTKTKIDRVKIFDTNPDILRAF 69

Query: 79  AGTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLI 138
           AG+GILVTVTVGNGEIPAL +LA AR WVA HIAPFHPQTRIHYIAVGNEI+HSNDKPLI
Sbjct: 70  AGSGILVTVTVGNGEIPALGNLAFARAWVAEHIAPFHPQTRIHYIAVGNEIIHSNDKPLI 129

Query: 139 ARLVPAMRAIHRALVLAGITDIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPMLQFL 198
           ARLVPAMR++HRALVLAGI DIKVTSPHSLGILSISEPPSMGRFRRGYDR +F PMLQFL
Sbjct: 130 ARLVPAMRSLHRALVLAGIWDIKVTSPHSLGILSISEPPSMGRFRRGYDRAIFGPMLQFL 189

Query: 199 RETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMDALHSAMKAL 258
           RETKAPFMVNPYPYF +SPKM NY LF+PNRGIYDK+T+I YTNMFD MMDA++SAMK L
Sbjct: 190 RETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTRITYTNMFDSMMDAIYSAMKKL 249

Query: 259 GFADVDLVIGETGWPTSCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFA 318
           G+AD+ +VIGETGWPT+CD Y AC+P NAAT+NRNL+WHVNSGKGTPLMPNRKFETYLF 
Sbjct: 250 GYADIKIVIGETGWPTNCD-YIACSPDNAATFNRNLVWHVNSGKGTPLMPNRKFETYLFG 309

Query: 319 LFNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGGQRRPTPVAPSGKKWCV 378
           LFNENLKPGP AERNWGLFQPDFTPVY+LPNIM GGR GRR G +  PTP+APS KKWCV
Sbjct: 310 LFNENLKPGPTAERNWGLFQPDFTPVYNLPNIMLGGR-GRR-GRRPSPTPIAPSAKKWCV 369

Query: 379 PRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSYYQSSGRNDFN 438
           PRS AA+EALQKNIDYVCSS ++CRPIQAGGACFLPND+RSHA+Y MN+YYQ+SGR+D+N
Sbjct: 370 PRSGAAVEALQKNIDYVCSSKVDCRPIQAGGACFLPNDVRSHAAYVMNAYYQTSGRHDYN 429

Query: 439 CDFGHTGVLTTVDPSHGGCAYAS 462
           CDFG TGVLTTV+PSHGGC Y S
Sbjct: 430 CDFGQTGVLTTVNPSHGGCVYVS 449

[Close]

BLAST of Lcy13g008730.1 vs. ExPASy TrEMBL
Match: A0A1S3CF05 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103500207 PE=3 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 4.6e-221
Identity = 366/443 (82.62%), Postives = 405/443 (91.42%), Query Frame = 0


Query: 19  AAVLLLLLQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAF 78
           +A + LLLQIST   AIG+NYGTL NNLRPPAEVVNF++TKTKIDRVK+FDTNP+ILRAF
Sbjct: 10  SAAIFLLLQISTTVFAIGVNYGTLGNNLRPPAEVVNFLKTKTKIDRVKIFDTNPDILRAF 69

Query: 79  AGTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLI 138
           AG+GILVTVTVGNGEIPAL +LA AR WVA HIAPFHPQTRIHYIAVGNEI+HSNDKPLI
Sbjct: 70  AGSGILVTVTVGNGEIPALGNLAFARAWVAEHIAPFHPQTRIHYIAVGNEIIHSNDKPLI 129

Query: 139 ARLVPAMRAIHRALVLAGITDIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPMLQFL 198
           ARLVPAMR++HRALVLAGI DIKVTSPHSLGILSISEPPSMGRFRRGYDR +F PMLQFL
Sbjct: 130 ARLVPAMRSLHRALVLAGIWDIKVTSPHSLGILSISEPPSMGRFRRGYDRAIFGPMLQFL 189

Query: 199 RETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMDALHSAMKAL 258
           RETKAPFMVNPYPYF +SPKM NY LF+PNRGIYDK+T+I YTNMFD MMDA++SAMK L
Sbjct: 190 RETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTRITYTNMFDSMMDAIYSAMKKL 249

Query: 259 GFADVDLVIGETGWPTSCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFA 318
           G+AD+ +VIGETGWPT+CD Y AC+P NAAT+NRNL+WHVNSGKGTPLMPNRKFETYLF 
Sbjct: 250 GYADIKIVIGETGWPTNCD-YIACSPDNAATFNRNLVWHVNSGKGTPLMPNRKFETYLFG 309

Query: 319 LFNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGGQRRPTPVAPSGKKWCV 378
           LFNENLKPGP AERNWGLFQPDFTPVY+LPNIM GGR GRR G +  PTP+APS KKWCV
Sbjct: 310 LFNENLKPGPTAERNWGLFQPDFTPVYNLPNIMLGGR-GRR-GRRPSPTPIAPSAKKWCV 369

Query: 379 PRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSYYQSSGRNDFN 438
           PRS AA+EALQKNIDYVCSS ++CRPIQAGGACFLPND+RSHA+Y MN+YYQ+SGR+D+N
Sbjct: 370 PRSGAAVEALQKNIDYVCSSKVDCRPIQAGGACFLPNDVRSHAAYVMNAYYQTSGRHDYN 429

Query: 439 CDFGHTGVLTTVDPSHGGCAYAS 462
           CDFG TGVLTTV+PSHGGC Y S
Sbjct: 430 CDFGQTGVLTTVNPSHGGCVYVS 449

[Close]

BLAST of Lcy13g008730.1 vs. ExPASy TrEMBL
Match: A0A0A0KS71 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G181480 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.3e-220
Identity = 371/445 (83.37%), Postives = 407/445 (91.46%), Query Frame = 0


Query: 14  MSAPA----AAVLLLLLQISTAAV-AIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLF 73
           MSA A    +A L LLLQIST AV AIG+NYGTL NNLRPPAEV NF++TKTKIDRVK+F
Sbjct: 1   MSAAALRRLSAALFLLLQISTTAVFAIGVNYGTLGNNLRPPAEVANFLKTKTKIDRVKIF 60

Query: 74  DTNPEILRAFAGTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNE 133
           DTNP+ILRAFAG+GILVTVTVGNG IP L+DLA AR+WVAAHIAPFHPQTRIHYIAVGNE
Sbjct: 61  DTNPDILRAFAGSGILVTVTVGNGLIPNLADLAFAREWVAAHIAPFHPQTRIHYIAVGNE 120

Query: 134 IMHSNDKPLIARLVPAMRAIHRALVLAGITDIKVTSPHSLGILSISEPPSMGRFRRGYDR 193
           I+HSNDKPLIARLVPAMR++HRALVLAGI D+KVTSPHSLGILSISEPPSMGRFRRGYDR
Sbjct: 121 IIHSNDKPLIARLVPAMRSLHRALVLAGIRDVKVTSPHSLGILSISEPPSMGRFRRGYDR 180

Query: 194 TVFTPMLQFLRETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMM 253
            +F PMLQFLRETKAPFMVNPYPYF +SPKM NY LF+PNRGIYDK+TKI YTNMFD MM
Sbjct: 181 AIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTKITYTNMFDSMM 240

Query: 254 DALHSAMKALGFADVDLVIGETGWPTSCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMP 313
           DA++SAMK LG+ADV++V+GETGWPT+CD Y AC+P NAATYNRNL+WHVNSGKGTPLMP
Sbjct: 241 DAIYSAMKKLGYADVNIVLGETGWPTNCD-YIACSPDNAATYNRNLVWHVNSGKGTPLMP 300

Query: 314 NRKFETYLFALFNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGGQRRPTP 373
           NRKFETYLF LFNENLKPGP AERNWGLFQPDFTPVY LPNIM GG    RG G RRPTP
Sbjct: 301 NRKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYSLPNIMLGG----RGRGGRRPTP 360

Query: 374 VAPSGKKWCVPRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSY 433
           VAPSGKKWCVPRSDAA++ALQKNIDYVCSSN++CRPIQAGGACFLPND+RSHA+Y MNSY
Sbjct: 361 VAPSGKKWCVPRSDAAVDALQKNIDYVCSSNVDCRPIQAGGACFLPNDVRSHAAYVMNSY 420

Query: 434 YQSSGRNDFNCDFGHTGVLTTVDPS 454
           YQ+SGR+D+NCDF HTGVLTTV+PS
Sbjct: 421 YQTSGRHDYNCDFSHTGVLTTVNPS 440

[Close]

BLAST of Lcy13g008730.1 vs. NCBI nr
Match: XP_038891662.1 (glucan endo-1,3-beta-glucosidase, partial [Benincasa hispida])

HSP 1 Score: 826.6 bits (2134), Expect = 1.0e-235
Identity = 399/461 (86.55%), Postives = 427/461 (92.62%), Query Frame = 0


Query: 8   LRRRPTMSAPAA-----AVLLLLLQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKI 67
           L RRP MS+ AA     A L LLLQISTA VAIG+NYGTLANNLRPPAEVVNF++TKTKI
Sbjct: 49  LLRRPIMSSAAAFHRISAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKI 108

Query: 68  DRVKLFDTNPEILRAFAGTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHY 127
           DRVK+FDTNP+ILRAFAG+GILVTVTVGNGEIPAL DLA ARQWVAAHIAPFHP+TRIHY
Sbjct: 109 DRVKIFDTNPDILRAFAGSGILVTVTVGNGEIPALGDLAVARQWVAAHIAPFHPRTRIHY 168

Query: 128 IAVGNEIMHSNDKPLIARLVPAMRAIHRALVLAGITDIKVTSPHSLGILSISEPPSMGRF 187
           IAVGNEI+HSNDKPLIARLVPAMR++HRALVLAGI D+KVTSPHSLGILSISEPPSMGRF
Sbjct: 169 IAVGNEIIHSNDKPLIARLVPAMRSLHRALVLAGIRDVKVTSPHSLGILSISEPPSMGRF 228

Query: 188 RRGYDRTVFTPMLQFLRETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTN 247
           RRGYDRT+F PMLQFLRETKAPFMVNPYPYF +SPKM NYALFRPNRGIYDK+TKI YTN
Sbjct: 229 RRGYDRTIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFRPNRGIYDKYTKITYTN 288

Query: 248 MFDGMMDALHSAMKALGFADVDLVIGETGWPTSCDGYFACNPQNAATYNRNLIWHVNSGK 307
           MFDGMMDAL+SAMK LG+ADVDLVIGETGWPT+CD Y AC+P NAATYNRNLIWHVNSGK
Sbjct: 289 MFDGMMDALYSAMKKLGYADVDLVIGETGWPTNCD-YIACSPDNAATYNRNLIWHVNSGK 348

Query: 308 GTPLMPNRKFETYLFALFNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGG 367
           GTPLMPNRKFETYLF LFNENLKPGP AERNWGLFQPDFTPVYDLPNIM G R G RGGG
Sbjct: 349 GTPLMPNRKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGRRRGGRGGG 408

Query: 368 QR--RPTPVAPSGKKWCVPRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSH 427
           +R   PTPVAPSG+KWCVPRSDA IEALQKNIDYVCSSNI+CRPIQAGGACFLPND+RSH
Sbjct: 409 RRTPSPTPVAPSGQKWCVPRSDAPIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSH 468

Query: 428 ASYAMNSYYQSSGRNDFNCDFGHTGVLTTVDPSHGGCAYAS 462
           A+Y MNSYYQ++GR+DFNCDFGHTGVLTTV+PSHGGCAY S
Sbjct: 469 AAYVMNSYYQTAGRHDFNCDFGHTGVLTTVNPSHGGCAYVS 508

[Close]

BLAST of Lcy13g008730.1 vs. NCBI nr
Match: XP_022992969.1 (glucan endo-1,3-beta-glucosidase [Cucurbita maxima])

HSP 1 Score: 799.3 bits (2063), Expect = 1.8e-227
Identity = 377/442 (85.29%), Postives = 414/442 (93.67%), Query Frame = 0


Query: 20  AVLLLLLQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAFA 79
           A LL+LLQISTAAVAIG+NYGTLANNLRPPAEVVNFI+TKTKIDRVK+FDTNP+ILRAFA
Sbjct: 11  AALLILLQISTAAVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAFA 70

Query: 80  GTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLIA 139
           GTGILVTVTVGNGEIPAL+DLA ARQWVAAHI PFHPQTRI+YIAVGNEI+H+NDKPLIA
Sbjct: 71  GTGILVTVTVGNGEIPALTDLATARQWVAAHIRPFHPQTRINYIAVGNEIIHTNDKPLIA 130

Query: 140 RLVPAMRAIHRALVLAGITDIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPMLQFLR 199
           RLVPAM+++HRALVLAGITDIKVTSPHSL ILSISEPPSMGRFRRGYDR +F PMLQFLR
Sbjct: 131 RLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRAIFGPMLQFLR 190

Query: 200 ETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMDALHSAMKALG 259
           ETKAPFMVNPYPYF +SP M NYALF PNRG+YDK+TKI YTNMFD MMDAL+SAMK +G
Sbjct: 191 ETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMDALYSAMKNIG 250

Query: 260 FADVDLVIGETGWPTSCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFAL 319
           ++DVDLVIGETGWP++CDGY AC+P NAAT++RNL+WHVNSGKGTPLMPNRKFETYLFAL
Sbjct: 251 YSDVDLVIGETGWPSNCDGYVACSPGNAATFHRNLVWHVNSGKGTPLMPNRKFETYLFAL 310

Query: 320 FNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGGQRRPTPVAPSGKKWCVP 379
           FNENLKPGP AERNWGLF PDFTPVYD+PNIM GGR+  RGG   RP PVAPSGKKWCVP
Sbjct: 311 FNENLKPGPTAERNWGLFHPDFTPVYDVPNIMIGGRH--RGGRGGRPKPVAPSGKKWCVP 370

Query: 380 RSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSYYQSSGRNDFNC 439
           +SDA+++ALQKNIDYVCSSNI+CRPIQAGGACFLPNDIR+HASYAMNSYYQ+SGRNDFNC
Sbjct: 371 KSDASVQALQKNIDYVCSSNIDCRPIQAGGACFLPNDIRAHASYAMNSYYQTSGRNDFNC 430

Query: 440 DFGHTGVLTTVDPSHGGCAYAS 462
           DF  TGV+TTV+PSHGGC Y S
Sbjct: 431 DFSLTGVITTVNPSHGGCVYIS 450

[Close]

BLAST of Lcy13g008730.1 vs. NCBI nr
Match: KAG6601002.1 (Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 798.1 bits (2060), Expect = 4.0e-227
Identity = 378/443 (85.33%), Postives = 413/443 (93.23%), Query Frame = 0


Query: 20  AVLLLLLQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAFA 79
           A LL+LLQISTAAVAIG+NYGTL NNLRPPAEVVNFI+TKTKIDRVK+FDTNP+ILRAFA
Sbjct: 11  AALLILLQISTAAVAIGVNYGTLGNNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAFA 70

Query: 80  GTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLIA 139
           GTGILVTVTVGNGEIP L+DLA ARQWVAAHI PFHPQTRIHYIAVGNEI+H+NDKPLIA
Sbjct: 71  GTGILVTVTVGNGEIPGLTDLATARQWVAAHIRPFHPQTRIHYIAVGNEIIHTNDKPLIA 130

Query: 140 RLVPAMRAIHRALVLAGITDIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPMLQFLR 199
           RLVPAM+++HRALVLAGITDIKVTSPHSL ILSISEPPS+GRFRRGYDR +F PMLQFLR
Sbjct: 131 RLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSLGRFRRGYDRAIFAPMLQFLR 190

Query: 200 ETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMDALHSAMKALG 259
           ETKAPFMVNPYPYF +SP M NYALF PNRG+YDK+TKI YTNMFD MMDAL+SAMK +G
Sbjct: 191 ETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMDALYSAMKNIG 250

Query: 260 FADVDLVIGETGWPTSCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFAL 319
           ++DVDLVIGETGWPT+CD Y AC+P NAAT++RNL+WHVNSGKGTPLMPNRKFETYLFAL
Sbjct: 251 YSDVDLVIGETGWPTNCD-YVACSPNNAATFHRNLVWHVNSGKGTPLMPNRKFETYLFAL 310

Query: 320 FNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRN-GRRGGGQRRPTPVAPSGKKWCV 379
           FNENLKPGP AERNWGLF PDFTPVYD+PNIM GGR+ G RGGG   P PVAPSGKKWCV
Sbjct: 311 FNENLKPGPTAERNWGLFYPDFTPVYDVPNIMIGGRHRGGRGGG---PKPVAPSGKKWCV 370

Query: 380 PRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSYYQSSGRNDFN 439
           P+SDA+++ALQKNIDYVCSSNI+CRPIQAGGACFLPNDIRSHASYAMNSYYQ+SGRNDFN
Sbjct: 371 PKSDASVQALQKNIDYVCSSNIDCRPIQAGGACFLPNDIRSHASYAMNSYYQTSGRNDFN 430

Query: 440 CDFGHTGVLTTVDPSHGGCAYAS 462
           CDF HTGV+TTV+PSHGGC Y S
Sbjct: 431 CDFSHTGVITTVNPSHGGCVYIS 449

[Close]

BLAST of Lcy13g008730.1 vs. NCBI nr
Match: XP_022957586.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita moschata])

HSP 1 Score: 795.8 bits (2054), Expect = 2.0e-226
Identity = 377/443 (85.10%), Postives = 412/443 (93.00%), Query Frame = 0


Query: 20  AVLLLLLQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAFA 79
           A LL+LLQISTAAVAIG+NYGTL NNLRPPAEVVNFI+TKTKIDRVK+FDTNP+ILRAFA
Sbjct: 11  AALLILLQISTAAVAIGVNYGTLGNNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAFA 70

Query: 80  GTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLIA 139
           G+GILVTVTVGNGEIP L+DLA ARQWVAAHI PFHPQTRIHYIAVGNEI+H+NDKPLIA
Sbjct: 71  GSGILVTVTVGNGEIPGLTDLATARQWVAAHIRPFHPQTRIHYIAVGNEIIHTNDKPLIA 130

Query: 140 RLVPAMRAIHRALVLAGITDIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPMLQFLR 199
           RLVPAM+++HRALVLAGITDIKVTSPHSL ILSISEPPSMGRFRRGYDR +F PMLQFLR
Sbjct: 131 RLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRAIFAPMLQFLR 190

Query: 200 ETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMDALHSAMKALG 259
           ETKAPFMVNPYPYF +SP M NYALF PNRG+YDK+TKI YTNMFD MMDAL+SAMK +G
Sbjct: 191 ETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMDALYSAMKNIG 250

Query: 260 FADVDLVIGETGWPTSCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFAL 319
           ++DVDLVIGETGWPT+CD Y AC+P NAAT++RNL+WHVNSGKGTPLMPNRKFETYLFAL
Sbjct: 251 YSDVDLVIGETGWPTNCD-YVACSPNNAATFHRNLVWHVNSGKGTPLMPNRKFETYLFAL 310

Query: 320 FNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRN-GRRGGGQRRPTPVAPSGKKWCV 379
           FNENLKPGP AERNWGLF PDFTPVYD+PNIM GGR+ G RGGG   P PVAPSGKKWCV
Sbjct: 311 FNENLKPGPTAERNWGLFYPDFTPVYDVPNIMIGGRHRGGRGGG---PKPVAPSGKKWCV 370

Query: 380 PRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSYYQSSGRNDFN 439
           P+SDA+++ALQ NIDYVCSSNI+CRPIQAGGACFLPNDIRSHASYAMNSYYQ+SGRNDFN
Sbjct: 371 PKSDASVQALQNNIDYVCSSNIDCRPIQAGGACFLPNDIRSHASYAMNSYYQTSGRNDFN 430

Query: 440 CDFGHTGVLTTVDPSHGGCAYAS 462
           CDF HTGV+TTV+PSHGGC Y S
Sbjct: 431 CDFSHTGVITTVNPSHGGCVYIS 449

[Close]

BLAST of Lcy13g008730.1 vs. NCBI nr
Match: XP_023526950.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 793.5 bits (2048), Expect = 9.7e-226
Identity = 376/443 (84.88%), Postives = 413/443 (93.23%), Query Frame = 0


Query: 20  AVLLLLLQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAFA 79
           A LL+LLQISTAAVAIG+NYGTLANNLRPPAEVVNFI+TKTKIDRVK+FDTNP+ILRAFA
Sbjct: 11  AALLILLQISTAAVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAFA 70

Query: 80  GTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLIA 139
           G+GILVTVTVGNGEIP L+DLA ARQWVAAHI PFHPQTRIHYIAVGNEI+H+NDKPLIA
Sbjct: 71  GSGILVTVTVGNGEIPGLTDLATARQWVAAHIRPFHPQTRIHYIAVGNEIIHTNDKPLIA 130

Query: 140 RLVPAMRAIHRALVLAGITDIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPMLQFLR 199
           RLVPAM+++HRALVLAGITDIKVTSPHSL ILSISEPPSMGRFRRGYDR +F PMLQFLR
Sbjct: 131 RLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRAIFGPMLQFLR 190

Query: 200 ETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMDALHSAMKALG 259
           ETKAPFMVNPYPYF +SP M NYALF PNRG+YDK+TKI YTNMFD MMDAL+SAMK +G
Sbjct: 191 ETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMDALYSAMKNIG 250

Query: 260 FADVDLVIGETGWPTSCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFAL 319
           ++DV++VIGETGWPT+CD Y AC+  NAAT++RNL+WHVNSGKGTPLMPNRKFETYLFAL
Sbjct: 251 YSDVEMVIGETGWPTNCD-YVACSTNNAATFHRNLVWHVNSGKGTPLMPNRKFETYLFAL 310

Query: 320 FNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRN-GRRGGGQRRPTPVAPSGKKWCV 379
           FNENLKPGP AERNWGLF PDFTPVYD+PNIM GGRN G RGGG   P PVAPSGKKWCV
Sbjct: 311 FNENLKPGPTAERNWGLFYPDFTPVYDVPNIMIGGRNRGGRGGG---PKPVAPSGKKWCV 370

Query: 380 PRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSYYQSSGRNDFN 439
           P+SDA+++ALQKNIDYVCSSNI+CRPIQAGGACFLPNDIRSHAS+AMNSYYQ+SGRNDFN
Sbjct: 371 PKSDASVQALQKNIDYVCSSNIDCRPIQAGGACFLPNDIRSHASFAMNSYYQTSGRNDFN 430

Query: 440 CDFGHTGVLTTVDPSHGGCAYAS 462
           CDF HTGV+TTV+PSHGGC Y S
Sbjct: 431 CDFSHTGVITTVNPSHGGCVYIS 449

[Close]

BLAST of Lcy13g008730.1 vs. TAIR 10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 501.1 bits (1289), Expect = 9.3e-142
Identity = 251/453 (55.41%), Postives = 331/453 (73.07%), Query Frame = 0


Query: 17  PAAAVLLLL-----LQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTN 76
           P+ ++LLLL     L I     AIG+NYGTL  NL PP +V NFI+T+T ID VK+FD N
Sbjct: 6   PSISLLLLLCAAVFLTIPAVISAIGVNYGTL-GNLPPPTQVANFIKTQTSIDSVKIFDVN 65

Query: 77  PEILRAFAGTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIMH 136
           P+ILRAFAGTGI V VTV NG+IPAL++   AR+WV+ +I PFHPQT+I YI+VGNEI+ 
Sbjct: 66  PDILRAFAGTGISVVVTVPNGDIPALANGRQARRWVSVNILPFHPQTKIKYISVGNEILL 125

Query: 137 SNDKPLIARLVPAMRAIHRALVLAGITDIKVTSPHSLGILS--ISEPPSMGRFRRGYDRT 196
           + D  +I  L+PAMR ++ ALV AG+ D+KVT+ HSL I++  ++  PS GRFR G+D+ 
Sbjct: 126 TGDNNMINNLLPAMRNLNNALVRAGVRDVKVTTAHSLNIIAYDLTGAPSSGRFRPGWDKG 185

Query: 197 VFTPMLQFLRETKAPFMVNPYPYFAFSPKMPNYALFR-PNRGIYDKFTKIRYTNMFDGMM 256
           +  P+L + R TK+PFMVNPYPYF F PK  N+A+FR P + + D FT+  YTNMFD +M
Sbjct: 186 ILAPILAYHRRTKSPFMVNPYPYFGFDPKNVNFAIFRTPYKAVRDPFTRHVYTNMFDALM 245

Query: 257 DALHSAMKALGFADVDLVIGETGWPTSCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMP 316
           D+ +SAMKALG+ DV++V+GETGWP++CD  + C+P NAA +N N+I     G+GTPLMP
Sbjct: 246 DSTYSAMKALGYGDVNIVVGETGWPSACDAPW-CSPANAAWFNLNIIKRA-QGQGTPLMP 305

Query: 317 NRKFETYLFALFNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGGQRRPTP 376
           NR+FETY+F LFNE  KPGP AERNWGLF+ DF+PVYD+  +    RNG+ GGG  RP  
Sbjct: 306 NRRFETYIFGLFNEEGKPGPTAERNWGLFRADFSPVYDVGLL----RNGQGGGG--RPAL 365

Query: 377 VAPS--GKKWCVPRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMN 436
            APS  G KWCV RS A    LQ +I++VC   ++C+PIQAGG+CF P+ +R+HAS+ MN
Sbjct: 366 PAPSTAGGKWCVARSGATNTQLQDSINWVCGQGVDCKPIQAGGSCFNPSSLRTHASFVMN 425

Query: 437 SYYQSSGRNDFNCDFGHTGVLTTVDPSHGGCAY 460
           +Y+QS GR D  C+F  TG++   +PS+G C Y
Sbjct: 426 AYFQSHGRTDGACNFSGTGMIVGNNPSNGACKY 449

[Close]

BLAST of Lcy13g008730.1 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 475.7 bits (1223), Expect = 4.2e-134
Identity = 231/451 (51.22%), Postives = 320/451 (70.95%), Query Frame = 0


Query: 12  PTMSAPAAAVLLLLLQISTAAVA-IGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDT 71
           P  S     ++ +LL +ST + A IG+NYGTLANNL PP +V  F+   T I+R++LFDT
Sbjct: 6   PFFSLSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDT 65

Query: 72  NPEILRAFAGTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIM 131
           +P+IL+AFA TGI VTVTV N +IP L++L++A+QW++ HI P  P T I  I VGNE++
Sbjct: 66  DPQILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVI 125

Query: 132 HSNDKPLIARLVPAMRAIHRALVLAGI-TDIKVTSPHSLGILSISEPPSMGRFRRGYDRT 191
            + D  LI  L+PAM+++H ALV A +   I++++PHSLG L+ S PPS  +FRRGYD  
Sbjct: 126 STADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQ 185

Query: 192 VFTPMLQFLRETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMD 251
           V  P+L FLR T +PF+VNPYP+F +S +  ++ALFRPN G++D+ TK+ YTNM D  +D
Sbjct: 186 VLKPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLD 245

Query: 252 ALHSAMKALGFADVDLVIGETGWPTSCD-GYFACNPQNAATYNRNLIWHVNSGKGTPLMP 311
           +++SAM  LGF+DV++VIGE GWP+  D      +   AA +N+NLI  V+SG GTPLMP
Sbjct: 246 SVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMP 305

Query: 312 NRKFETYLFALFNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGGQRRPTP 371
           NR FETY+FALFNENLK GP +ERN+G+F+ D TP+YD+  +    R+       R P  
Sbjct: 306 NRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDPVYNPRSPVR 365

Query: 372 VAPSGKKWCVPRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSY 431
              S K+WCV ++ A   ALQ+NIDYVC   ++CRPI  GG C+LPN +++H+ YAMN Y
Sbjct: 366 -GSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLY 425

Query: 432 YQSSGRNDFNCDFGHTGVLTTVDPSHGGCAY 460
           YQ+  +++F+CDF +TG +TT+DPS+G C Y
Sbjct: 426 YQTMEKHEFDCDFDNTGEITTIDPSYGNCEY 455

[Close]

BLAST of Lcy13g008730.1 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 467.2 bits (1201), Expect = 1.5e-131
Identity = 228/445 (51.24%), Postives = 316/445 (71.01%), Query Frame = 0


Query: 12  PTMSAPAAAVLLLLLQISTAAVA-IGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDT 71
           P  S     ++ +LL +ST + A IG+NYGTLANNL PP +V  F+   T I+R++LFDT
Sbjct: 6   PFFSLSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDT 65

Query: 72  NPEILRAFAGTGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIM 131
           +P+IL+AFA TGI VTVTV N +IP L++L++A+QW++ HI P  P T I  I VGNE++
Sbjct: 66  DPQILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVI 125

Query: 132 HSNDKPLIARLVPAMRAIHRALVLAGI-TDIKVTSPHSLGILSISEPPSMGRFRRGYDRT 191
            + D  LI  L+PAM+++H ALV A +   I++++PHSLG L+ S PPS  +FRRGYD  
Sbjct: 126 STADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQ 185

Query: 192 VFTPMLQFLRETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMD 251
           V  P+L FLR T +PF+VNPYP+F +S +  ++ALFRPN G++D+ TK+ YTNM D  +D
Sbjct: 186 VLKPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLD 245

Query: 252 ALHSAMKALGFADVDLVIGETGWPTSCD-GYFACNPQNAATYNRNLIWHVNSGKGTPLMP 311
           +++SAM  LGF+DV++VIGE GWP+  D      +   AA +N+NLI  V+SG GTPLMP
Sbjct: 246 SVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMP 305

Query: 312 NRKFETYLFALFNENLKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGGQRRPTP 371
           NR FETY+FALFNENLK GP +ERN+G+F+ D TP+YD+  +    R+       R P  
Sbjct: 306 NRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDPVYNPRSPVR 365

Query: 372 VAPSGKKWCVPRSDAAIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSY 431
              S K+WCV ++ A   ALQ+NIDYVC   ++CRPI  GG C+LPN +++H+ YAMN Y
Sbjct: 366 -GSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLY 425

Query: 432 YQSSGRNDFNCDFGHTGVLTTVDPS 454
           YQ+  +++F+CDF +TG +TT+DPS
Sbjct: 426 YQTMEKHEFDCDFDNTGEITTIDPS 449

[Close]

BLAST of Lcy13g008730.1 vs. TAIR 10
Match: AT2G39640.1 (glycosyl hydrolase family 17 protein )

HSP 1 Score: 441.4 bits (1134), Expect = 8.8e-124
Identity = 217/434 (50.00%), Postives = 294/434 (67.74%), Query Frame = 0


Query: 24  LLLQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAFAGTGI 83
           ++ Q+S    AIGINYGTL  NL+PP +VV+FI+TKT  D VK++D NP+ILRAFAG+ I
Sbjct: 19  VIFQLSAVTSAIGINYGTL-GNLQPPQQVVDFIKTKTTFDSVKIYDANPDILRAFAGSEI 78

Query: 84  LVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLIARLVP 143
            +T+ V NG IPA+ ++ANARQWVAA++ PF  Q +  Y+ VGNEI+ SND  LI+ LVP
Sbjct: 79  NITIMVPNGNIPAMVNVANARQWVAANVLPFQQQIKFKYVCVGNEILASNDNNLISNLVP 138

Query: 144 AMRAIHRALVLAGITDIKVTSPHSLGILSISEPPSMGRFRRGYDRTVFTPMLQFLRETKA 203
           AM++++ AL  + +T IKVT+PH+  I      PS  RF     + +FT +L+F R+ K+
Sbjct: 139 AMQSLNEALKASNLTYIKVTTPHAFTISYNRNTPSESRFTND-QKDIFTKILEFHRQAKS 198

Query: 204 PFMVNPYPYFAFSPKMPNYALFRPNRGIYDKFTKIRYTNMFDGMMDALHSAMKALGFADV 263
           PFM+N Y +F       NYA+F P+  I D  T+  YTNMFD +MDA +SAMKALG+ DV
Sbjct: 199 PFMINAYTFFTMDTNNVNYAIFGPSNAITDTNTQQTYTNMFDAVMDATYSAMKALGYGDV 258

Query: 264 DLVIGETGWPTSCDGYFACNPQNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFALFNEN 323
           D+ +GETGWPT+CD  + C+PQNA  YN N+I       GTPLMPNR  + ++FALFNE+
Sbjct: 259 DIAVGETGWPTACDASW-CSPQNAENYNLNIIKRAQV-IGTPLMPNRHIDIFIFALFNED 318

Query: 324 LKPGPDAERNWGLFQPDFTPVYDLPNIMRGGRNGRRGGGQRRPTPVAPSGKKWCVPRSDA 383
            KPGP  ERNWG+F+PDF+P+YD+  +        +GGG   P P   +  KWCV + +A
Sbjct: 319 GKPGPTRERNWGIFKPDFSPMYDVGVL--------KGGGSPLPFPPINNNGKWCVGKPEA 378

Query: 384 AIEALQKNIDYVCSSNINCRPIQAGGACFLPNDIRSHASYAMNSYYQSSGRNDFNCDFGH 443
            +  LQ NID+VCS  I+C PI  GG CF  N++ + +S+ MN+YYQS G  D  CDF  
Sbjct: 379 TLMQLQANIDWVCSHGIDCTPISPGGICFDNNNMTTRSSFIMNAYYQSKGCVDVVCDFSG 438

Query: 444 TGVLTTVDPSHGGC 458
           TG++T+ +PS   C
Sbjct: 439 TGIVTSTNPSTSTC 440

[Close]

BLAST of Lcy13g008730.1 vs. TAIR 10
Match: AT3G46570.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.6e-104
Identity = 193/331 (58.31%), Postives = 248/331 (74.92%), Query Frame = 0


Query: 21  VLLLLLQISTAAVAIGINYGTLANNLRPPAEVVNFIRTKTKIDRVKLFDTNPEILRAFAG 80
           +L L+L  S AA  +G+NYGT+ANNL PP +VVNFI+TKT I+ VK+FDTN +IL AF+G
Sbjct: 14  ILTLILPFS-AAYQVGVNYGTVANNLPPPHQVVNFIKTKTIINHVKIFDTNHDILAAFSG 73

Query: 81  -TGILVTVTVGNGEIPALSDLANARQWVAAHIAPFHPQTRIHYIAVGNEIMHSNDKPLIA 140
            TGI +TVTV N +I +LS L+NAR W++ ++ PF   T I YIAVGNE++ ++DK LI 
Sbjct: 74  ITGISLTVTVPNSDIISLSKLSNARSWLSDNLLPFLLTTSIRYIAVGNEVVATSDKTLIT 133

Query: 141 RLVPAMRAIHRALVLAGITDIKVTSPHSLGILS-ISEPPSMGRFRRGYDRTVFTPMLQFL 200
            L+PAM  +  AL LA ++ I V++PHSLGILS  SEPPS G+FR+GYD+ +F+P+L F 
Sbjct: 134 HLLPAMETLTLALHLANVSRILVSTPHSLGILSGSSEPPSSGKFRKGYDKAIFSPILDFH 193

Query: 201 RETKAPFMVNPYPYFAFSPKMPNYALFRPNRGIY-DKFTKIRYTNMFDGMMDALHSAMKA 260
             TK+PFMVNPYPYF F P+  NYALF  N  +Y D  TK+ YTNMFD  +DA++SAMK 
Sbjct: 194 NRTKSPFMVNPYPYFGFGPETLNYALFNTNDVVYVDPVTKLNYTNMFDAQLDAVYSAMKR 253

Query: 261 LGFADVDLVIGETGWPTSCD-GYFACNPQNAATYNRNLIWHVNSGKGTPLMPNRKFETYL 320
            G+ DVD+V+ ETGWP++ +          AA YN NLI HVNSGKGTPLMPNR FETY+
Sbjct: 254 FGYGDVDIVVAETGWPSAGEPNQTGVGLDYAAAYNGNLIKHVNSGKGTPLMPNRVFETYV 313

Query: 321 FALFNENLKPGPDAERNWGLFQPDFTPVYDL 348
           F+LFNENLK    +E+N+GLF+PDFTPVYD+
Sbjct: 314 FSLFNENLKSSV-SEQNFGLFKPDFTPVYDV 342

The following BLAST results are available for this feature:

BLAST of Lcy13g008730.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52409	2.8e-127	50.33	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]
Q9M069	2.4e-102	43.44	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]
Q94G86	3.1e-97	41.46	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]
Q8VYE5	2.0e-80	38.18	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q9FJU9	3.8e-79	39.60	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]

BLAST of Lcy13g008730.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JYX6	8.6e-228	85.29	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111489139 PE=...	[more]
A0A6J1GZM3	9.5e-227	85.10	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111458933 P...	[more]
A0A5D3BZM0	4.6e-221	82.62	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3CF05	4.6e-221	82.62	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103500207 PE=3 SV...	[more]
A0A0A0KS71	2.3e-220	83.37	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G181480 PE=3...	[more]

BLAST of Lcy13g008730.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891662.1	1.0e-235	86.55	glucan endo-1,3-beta-glucosidase, partial [Benincasa hispida]	[more]
XP_022992969.1	1.8e-227	85.29	glucan endo-1,3-beta-glucosidase [Cucurbita maxima]	[more]
KAG6601002.1	4.0e-227	85.33	Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022957586.1	2.0e-226	85.10	glucan endo-1,3-beta-glucosidase-like [Cucurbita moschata]	[more]
XP_023526950.1	9.7e-226	84.88	glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy13g008730.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55430.1	9.3e-142	55.41	O-Glycosyl hydrolases family 17 protein	[more]
AT5G24318.1	4.2e-134	51.22	O-Glycosyl hydrolases family 17 protein	[more]
AT5G24318.2	1.5e-131	51.24	O-Glycosyl hydrolases family 17 protein	[more]
AT2G39640.1	8.8e-124	50.00	glycosyl hydrolase family 17 protein	[more]
AT3G46570.1	1.6e-104	58.31	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 375..459 e-value: 1.5E-44 score: 164.0
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 375..445 e-value: 5.6E-20 score: 71.8
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 33..352 e-value: 4.1E-116 score: 389.4
None	No IPR available	GENE3D	1.20.58.1040		coord: 372..459 e-value: 1.1E-10 score: 43.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 352..373
None	No IPR available	PANTHER	PTHR32227:SF371	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE-LIKE	coord: 21..455
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 35..347 e-value: 1.8E-81 score: 274.2
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 21..455
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 35..347

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lcy13g008730	Lcy13g008730	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy13g008730.1.utr5p1	Lcy13g008730.1.utr5p1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy13g008730.1.exon1	Lcy13g008730.1.exon1	exon
Lcy13g008730.1.exon2	Lcy13g008730.1.exon2	exon
Lcy13g008730.1.exon3	Lcy13g008730.1.exon3	exon
Lcy13g008730.1.exon4	Lcy13g008730.1.exon4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy13g008730.1.cds1	Lcy13g008730.1.cds1	CDS
Lcy13g008730.1.cds2	Lcy13g008730.1.cds2	CDS
Lcy13g008730.1.cds3	Lcy13g008730.1.cds3	CDS
Lcy13g008730.1.cds4	Lcy13g008730.1.cds4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy13g008730.1.utr3p1	Lcy13g008730.1.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lcy13g008730.1	Lcy13g008730.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity