Lag0029137.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0029137.1 vs. NCBI nr
Match: KAG7032842.1 (hypothetical protein SDJN02_06892 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 315.8 bits (808), Expect = 2.4e-82
Identity = 160/186 (86.02%), Postives = 169/186 (90.86%), Query Frame = 0

Query: 1   MALDNCIQLSTAAAMCATTSIPKSHPFSSNFHQKTTLSLQRLNKFPTSTSRFSLFSSST- 60
           MALDNCIQLSTAAAM  TTSIPKSHPFSS FHQK TLSLQRLNKFPTST+RF  FS+ST 
Sbjct: 1   MALDNCIQLSTAAAMYGTTSIPKSHPFSSTFHQKPTLSLQRLNKFPTSTARFKSFSTSTS 60

Query: 61  ---LRKPTAVTCKARGAVDDVKEVTESSWNELVVGSQKTVLVEFWAPWCGPCKMIEPVIK 120
                K  +VTCKARGAVDDVKEVTESSWNELVV S+KTVLVEFWAPWCGPCKMIEPVI+
Sbjct: 61  VSSFGKSRSVTCKARGAVDDVKEVTESSWNELVVESEKTVLVEFWAPWCGPCKMIEPVIR 120

Query: 121 DLAQEYAGKIVCLKLNTDVSPNVASKYGIRSIPTVLFFKNGQKRESIIGAVPKSTLSATI 180
           +LAQ+YAGKIVCLKLNTD SPNVASKYGIRSIPTVLFFKNGQKRE +IGAVPKSTLSA+I
Sbjct: 121 ELAQDYAGKIVCLKLNTDASPNVASKYGIRSIPTVLFFKNGQKREGVIGAVPKSTLSASI 180

Query: 181 DKYLEV 183
           DKYLEV
Sbjct: 181 DKYLEV 186

BLAST of Lag0029137.1 vs. NCBI nr
Match: XP_022989806.1 (uncharacterized protein LOC111486882 [Cucurbita maxima])

HSP 1 Score: 315.1 bits (806), Expect = 4.0e-82
Identity = 160/186 (86.02%), Postives = 168/186 (90.32%), Query Frame = 0

Query: 1   MALDNCIQLSTAAAMCATTSIPKSHPFSSNFHQKTTLSLQRLNKFPTSTSRFSLFSS--- 60
           MALDNCIQLSTAAAM  TTSIPKSHPFSS FHQKTTLSLQRLNKFPTS +RF  FS    
Sbjct: 1   MALDNCIQLSTAAAMYGTTSIPKSHPFSSTFHQKTTLSLQRLNKFPTSIARFKSFSPSTS 60

Query: 61  -STLRKPTAVTCKARGAVDDVKEVTESSWNELVVGSQKTVLVEFWAPWCGPCKMIEPVIK 120
            ST  K  +VTCKARGAVDDVKEVTESSWNELVV S+KTVLVEFWAPWCGPCKMIEPVI+
Sbjct: 61  VSTFGKSRSVTCKARGAVDDVKEVTESSWNELVVESEKTVLVEFWAPWCGPCKMIEPVIR 120

Query: 121 DLAQEYAGKIVCLKLNTDVSPNVASKYGIRSIPTVLFFKNGQKRESIIGAVPKSTLSATI 180
           +LAQ+YAGKIVCLKLNTD SPNVASKYGIRSIPTVLFFKNGQKRE +IGAVPKSTLSA+I
Sbjct: 121 ELAQDYAGKIVCLKLNTDASPNVASKYGIRSIPTVLFFKNGQKREGVIGAVPKSTLSASI 180

Query: 181 DKYLEV 183
           DKYLEV
Sbjct: 181 DKYLEV 186

BLAST of Lag0029137.1 vs. NCBI nr
Match: XP_022955939.1 (uncharacterized protein LOC111457772 [Cucurbita moschata])

HSP 1 Score: 315.1 bits (806), Expect = 4.0e-82
Identity = 160/186 (86.02%), Postives = 168/186 (90.32%), Query Frame = 0

Query: 1   MALDNCIQLSTAAAMCATTSIPKSHPFSSNFHQKTTLSLQRLNKFPTSTSRFSLFSS--- 60
           MALDNCIQLSTAAAM  TTSIPKSHPFSS FHQK TLSLQRLNKFPTST+RF  FS    
Sbjct: 1   MALDNCIQLSTAAAMYGTTSIPKSHPFSSTFHQKPTLSLQRLNKFPTSTARFKSFSPSTS 60

Query: 61  -STLRKPTAVTCKARGAVDDVKEVTESSWNELVVGSQKTVLVEFWAPWCGPCKMIEPVIK 120
            ST  K  +VTCKARGAVDDVKEVTESSWNELVV S+KTVLVEFWAPWCGPCKMIEPVI+
Sbjct: 61  VSTFGKSRSVTCKARGAVDDVKEVTESSWNELVVESEKTVLVEFWAPWCGPCKMIEPVIR 120

Query: 121 DLAQEYAGKIVCLKLNTDVSPNVASKYGIRSIPTVLFFKNGQKRESIIGAVPKSTLSATI 180
           +LAQ+YAGKIVCLKLNTD SPNVASKYGIRSIPTVLFFKNGQKRE +IGAVPKSTLSA+I
Sbjct: 121 ELAQDYAGKIVCLKLNTDASPNVASKYGIRSIPTVLFFKNGQKREGVIGAVPKSTLSASI 180

Query: 181 DKYLEV 183
           DKYLEV
Sbjct: 181 DKYLEV 186

BLAST of Lag0029137.1 vs. NCBI nr
Match: KAG6602152.1 (hypothetical protein SDJN03_07385, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 313.9 bits (803), Expect = 8.9e-82
Identity = 159/186 (85.48%), Postives = 168/186 (90.32%), Query Frame = 0

Query: 1   MALDNCIQLSTAAAMCATTSIPKSHPFSSNFHQKTTLSLQRLNKFPTSTSRFSLFSSST- 60
           MALDNCIQLSTAAAM  TTSIPKSHPFSS FHQK TLSLQRLNKFPT+T+RF  FS ST 
Sbjct: 1   MALDNCIQLSTAAAMYGTTSIPKSHPFSSTFHQKPTLSLQRLNKFPTTTARFKSFSPSTS 60

Query: 61  ---LRKPTAVTCKARGAVDDVKEVTESSWNELVVGSQKTVLVEFWAPWCGPCKMIEPVIK 120
                K  +VTCKARGAVDDVKEVTESSWNELVV S+KTVLVEFWAPWCGPCKMIEPVI+
Sbjct: 61  VSSFGKSRSVTCKARGAVDDVKEVTESSWNELVVESEKTVLVEFWAPWCGPCKMIEPVIR 120

Query: 121 DLAQEYAGKIVCLKLNTDVSPNVASKYGIRSIPTVLFFKNGQKRESIIGAVPKSTLSATI 180
           +LAQ+YAGKIVCLKLNTD SPNVASKYGIRSIPTVLFFKNGQKRE +IGAVPKSTLSA+I
Sbjct: 121 ELAQDYAGKIVCLKLNTDASPNVASKYGIRSIPTVLFFKNGQKREGVIGAVPKSTLSASI 180

Query: 181 DKYLEV 183
           DKYLEV
Sbjct: 181 DKYLEV 186

BLAST of Lag0029137.1 vs. NCBI nr
Match: XP_023524932.1 (uncharacterized protein LOC111788711 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 313.5 bits (802), Expect = 1.2e-81
Identity = 159/186 (85.48%), Postives = 167/186 (89.78%), Query Frame = 0

Query: 1   MALDNCIQLSTAAAMCATTSIPKSHPFSSNFHQKTTLSLQRLNKFPTSTSRFSLFSS--- 60
           MALDNCIQLSTAAAM  TTSIPKSHPFSS FHQK TLSLQR NKFPTST+RF  FS    
Sbjct: 1   MALDNCIQLSTAAAMYGTTSIPKSHPFSSTFHQKPTLSLQRFNKFPTSTARFKSFSPSTS 60

Query: 61  -STLRKPTAVTCKARGAVDDVKEVTESSWNELVVGSQKTVLVEFWAPWCGPCKMIEPVIK 120
            ST  K  +VTCKARGAVDDVKEVTESSWNELVV S+KTVLVEFWAPWCGPCKMIEPVI+
Sbjct: 61  VSTFGKSRSVTCKARGAVDDVKEVTESSWNELVVESEKTVLVEFWAPWCGPCKMIEPVIR 120

Query: 121 DLAQEYAGKIVCLKLNTDVSPNVASKYGIRSIPTVLFFKNGQKRESIIGAVPKSTLSATI 180
           +LAQ+YAGKIVCLKLNTD SPNVASKYGIRSIPTVLFFKNGQKRE +IGAVPKSTLSA+I
Sbjct: 121 ELAQDYAGKIVCLKLNTDASPNVASKYGIRSIPTVLFFKNGQKREGVIGAVPKSTLSASI 180

Query: 181 DKYLEV 183
           DKYLEV
Sbjct: 181 DKYLEV 186

BLAST of Lag0029137.1 vs. ExPASy Swiss-Prot
Match: P07591 (Thioredoxin M-type, chloroplastic OS=Spinacia oleracea OX=3562 PE=1 SV=2)

HSP 1 Score: 179.9 bits (455), Expect = 2.6e-44
Identity = 98/184 (53.26%), Postives = 131/184 (71.20%), Query Frame = 0

Query: 1   MALDNCIQLSTAAAMCATTSIPKSHPF----SSNFHQKTTLSLQRLNKFPTSTSRFSLFS 60
           MA++NC+QLST+A++   T   KSH      SS  +  T   L+R   FP  +S  S  S
Sbjct: 1   MAIENCLQLSTSASV--GTVAVKSHVHHLQPSSKVNVPTFRGLKR--SFPALSSSVSSSS 60

Query: 61  SSTLRKPTAVTCKARGAVDDVKEVTESSWNELVVGSQKTVLVEFWAPWCGPCKMIEPVIK 120
               R  ++V CKA  AV +V++V +SSW E V+ S+  V+V+FWAPWCGPCK+I PVI 
Sbjct: 61  PRQFRY-SSVVCKASEAVKEVQDVNDSSWKEFVLESEVPVMVDFWAPWCGPCKLIAPVID 120

Query: 121 DLAQEYAGKIVCLKLNTDVSPNVASKYGIRSIPTVLFFKNGQKRESIIGAVPKSTLSATI 180
           +LA+EY+GKI   KLNTD +P +A++Y IRSIPTVLFFKNG+++ESIIGAVPKSTL+ +I
Sbjct: 121 ELAKEYSGKIAVYKLNTDEAPGIATQYNIRSIPTVLFFKNGERKESIIGAVPKSTLTDSI 179

BLAST of Lag0029137.1 vs. ExPASy Swiss-Prot
Match: P48384 (Thioredoxin M-type, chloroplastic OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 5.0e-43
Identity = 87/164 (53.05%), Postives = 116/164 (70.73%), Query Frame = 0

Query: 30  NFHQKTTLS-------------LQRLNKFPTSTSRFSLFSSSTLRKPTAVTCKARGAVDD 89
           + H KT+LS             L   ++   S +  + F+S  L     V   AR AV++
Sbjct: 9   SIHTKTSLSSSIVFIFKGKACLLTSKSRIQESFAELNSFTSLVLLIENHVLLHAREAVNE 68

Query: 90  VKEVTESSWNELVVGSQKTVLVEFWAPWCGPCKMIEPVIKDLAQEYAGKIVCLKLNTDVS 149
           V+ V +SSW+ELV+GS+  VLV+FWAPWCGPC+MI P+I +LA+EYAGKI C KLNTD S
Sbjct: 69  VQVVNDSSWDELVIGSETPVLVDFWAPWCGPCRMIAPIIDELAKEYAGKIKCYKLNTDES 128

Query: 150 PNVASKYGIRSIPTVLFFKNGQKRESIIGAVPKSTLSATIDKYL 181
           PN A+KYGIRSIPTVLFFKNG++++S+IGAVPK+TLS  ++KY+
Sbjct: 129 PNTATKYGIRSIPTVLFFKNGERKDSVIGAVPKATLSEKVEKYI 172

BLAST of Lag0029137.1 vs. ExPASy Swiss-Prot
Match: Q9ZP20 (Thioredoxin M5, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TRXM PE=2 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 5.5e-42
Identity = 79/133 (59.40%), Postives = 100/133 (75.19%), Query Frame = 0

Query: 48  STSRFSLFSSSTLRKPTAVTCKARGAVDDVKEVTESSWNELVVGSQKTVLVEFWAPWCGP 107
           S  R S+ S S LR  +   C+    VD+V    E +W+ +V+GS+  VLVEFWAPWCGP
Sbjct: 38  SKPRLSVASPSPLRPASRFACQCSNVVDEVVVADEKNWDSMVLGSEAPVLVEFWAPWCGP 97

Query: 108 CKMIEPVIKDLAQEYAGKIVCLKLNTDVSPNVASKYGIRSIPTVLFFKNGQKRESIIGAV 167
           C+MI PVI +LA+EY GKI C K+NTD SPN+A+ YGIRSIPTVL FKNG+K+ES+IGAV
Sbjct: 98  CRMIAPVIDELAKEYVGKIKCCKVNTDDSPNIATNYGIRSIPTVLMFKNGEKKESVIGAV 157

Query: 168 PKSTLSATIDKYL 181
           PK+TL+  IDKY+
Sbjct: 158 PKTTLATIIDKYV 170

BLAST of Lag0029137.1 vs. ExPASy Swiss-Prot
Match: Q41864 (Thioredoxin M-type, chloroplastic OS=Zea mays OX=4577 GN=TRM1 PE=2 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.8e-40
Identity = 77/120 (64.17%), Postives = 96/120 (80.00%), Query Frame = 0

Query: 61  RKPTAVTCKARGAVDDVKEVTESSWNELVVGSQKTVLVEFWAPWCGPCKMIEPVIKDLAQ 120
           R+P  V C+++ AVD+V    E +W+ LV+  +  VLVEFWAPWCGPC+MI PVI +LA+
Sbjct: 46  RRPRHV-CQSKNAVDEVVVADEKNWDGLVMACETPVLVEFWAPWCGPCRMIAPVIDELAK 105

Query: 121 EYAGKIVCLKLNTDVSPNVASKYGIRSIPTVLFFKNGQKRESIIGAVPKSTLSATIDKYL 180
           +YAGKI C K+NTD SPNVAS YGIRSIPTVL FK G+K+ES+IGAVPKSTL+  IDKY+
Sbjct: 106 DYAGKITCCKVNTDDSPNVASTYGIRSIPTVLIFKGGEKKESVIGAVPKSTLTTLIDKYI 164

BLAST of Lag0029137.1 vs. ExPASy Swiss-Prot
Match: Q9ZP21 (Thioredoxin M-type, chloroplastic OS=Triticum aestivum OX=4565 PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 8.8e-40
Identity = 71/120 (59.17%), Postives = 92/120 (76.67%), Query Frame = 0

Query: 61  RKPTAVTCKARGAVDDVKEVTESSWNELVVGSQKTVLVEFWAPWCGPCKMIEPVIKDLAQ 120
           R+P+   CK +  VD+V    E +W+ +V+  +  VLVEFWAPWCGPC+MI PVI +LA+
Sbjct: 54  RRPSRFVCKCKNVVDEVIVADEKNWDNMVIACESPVLVEFWAPWCGPCRMIAPVIDELAK 113

Query: 121 EYAGKIVCLKLNTDVSPNVASKYGIRSIPTVLFFKNGQKRESIIGAVPKSTLSATIDKYL 180
           +Y GKI C K+NTD  PN+AS YGIRSIPTVL FK+G+K+ES+IGAVPK+TL   IDKY+
Sbjct: 114 DYVGKIKCCKVNTDDCPNIASTYGIRSIPTVLMFKDGEKKESVIGAVPKTTLCTIIDKYI 173