Lag0021556.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0021556.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	MLP-like protein 43
Location	chr7: 9122857 .. 9123375 (-)
Sequence length	423
RNA-Seq Expression	Lag0021556.1
Synteny	Lag0021556.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCACCACCATCACTCTCCTCGGAAAGTTGGAGATTGATGTAAAAATCAAGGCTCCGGCGTCCAAGGTTTATGAGATATTTCAGGAGAGACCTTACCTCCTCACTAAAATCTCTCCCGAAAATATACAAAGCTCTGAGTTACTAAAAGGCGAATGGGGCAAAGTTGGCTCTATCATTTATTGGACCTATACTCTCGGTTTGTTTCTAATCTTCTAATCATCTCTTTGAATTTCCTTTTCTTTTTAAAACCATTGCATTGAGGTTATATTTACTTGGTACTGTCGATTAATAGGTGGAAAAACTTCGGTAGTCAAACACATAGTTGAAGCTGTTGATGAAGAGAAAAATTTGATCACTTTCACATGGATAGAAGGAACCCTTTCGGAGTATTACAAAAAGTTTCAAGTTACAGTTCAATGTATTCCAAAACATATGGGAAGCGTGGTTCGTTGGATTACCGAATTTGAGATATTGCGCGTTGAGATTCCATATTTATGTAACTTGCCTCAGTTCTAA

mRNA sequence

ATGTCCACCACCATCACTCTCCTCGGAAAGTTGGAGATTGATGTAAAAATCAAGGCTCCGGCGTCCAAGGTTTATGAGATATTTCAGGAGAGACCTTACCTCCTCACTAAAATCTCTCCCGAAAATATACAAAGCTCTGAGTTACTAAAAGGCGAATGGGGCAAAGTTGGCTCTATCATTTATTGGACCTATACTCTCGGTGGAAAAACTTCGGTAGTCAAACACATAGTTGAAGCTGTTGATGAAGAGAAAAATTTGATCACTTTCACATGGATAGAAGGAACCCTTTCGGAGTATTACAAAAAGTTTCAAGTTACAGTTCAATGTATTCCAAAACATATGGGAAGCGTGGTTCGTTGGATTACCGAATTTGAGATATTGCGCGTTGAGATTCCATATTTATGTAACTTGCCTCAGTTCTAA

Coding sequence (CDS)

ATGTCCACCACCATCACTCTCCTCGGAAAGTTGGAGATTGATGTAAAAATCAAGGCTCCGGCGTCCAAGGTTTATGAGATATTTCAGGAGAGACCTTACCTCCTCACTAAAATCTCTCCCGAAAATATACAAAGCTCTGAGTTACTAAAAGGCGAATGGGGCAAAGTTGGCTCTATCATTTATTGGACCTATACTCTCGGTGGAAAAACTTCGGTAGTCAAACACATAGTTGAAGCTGTTGATGAAGAGAAAAATTTGATCACTTTCACATGGATAGAAGGAACCCTTTCGGAGTATTACAAAAAGTTTCAAGTTACAGTTCAATGTATTCCAAAACATATGGGAAGCGTGGTTCGTTGGATTACCGAATTTGAGATATTGCGCGTTGAGATTCCATATTTATGTAACTTGCCTCAGTTCTAA

Protein sequence

MSTTITLLGKLEIDVKIKAPASKVYEIFQERPYLLTKISPENIQSSELLKGEWGKVGSIIYWTYTLGGKTSVVKHIVEAVDEEKNLITFTWIEGTLSEYYKKFQVTVQCIPKHMGSVVRWITEFEILRVEIPYLCNLPQF

Homology

[Close]

BLAST of Lag0021556.1 vs. NCBI nr
Match: XP_022944436.1 (MLP-like protein 34 [Cucurbita moschata])

HSP 1 Score: 152.9 bits (385), Expect = 2.0e-33
Identity = 71/136 (52.21%), Postives = 97/136 (71.32%), Query Frame = 0


Query: 5   ITLLGKLEIDVKIKAPASKVYEIFQERPYLLTKISPENIQSSELLKGEWGKVGSIIYWTY 64
           ++LLGKLE DV I+APASK +EIF +RP+ ++ +S + IQ  E+ +GEWG+VGSI+YW Y
Sbjct: 1   MSLLGKLETDVDIEAPASKFHEIFHQRPHHISNVSSDKIQGCEIHEGEWGQVGSIVYWNY 60

Query: 65  TLGGKTSVVKHIVEAVDEEKNLITFTWIEGTLSEYYKKFQVTVQCIPKHMGSVVRWITEF 124
              GK  V K I+EAVDEE N+I+F  IEG L E YK F++T+ C+P+  GSV+ W  E+
Sbjct: 61  VHDGKPCVAKEIIEAVDEENNMISFKVIEGDLLEEYKSFKLTIHCLPRDKGSVIHWTLEY 120

Query: 125 EILRVEIPYLCNLPQF 141
           E L  E+P   +L QF
Sbjct: 121 EKLHDEVPDSHSLLQF 136

[Close]

BLAST of Lag0021556.1 vs. NCBI nr
Match: KAG6597607.1 (MLP-like protein 31, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 152.5 bits (384), Expect = 2.6e-33
Identity = 70/136 (51.47%), Postives = 98/136 (72.06%), Query Frame = 0


Query: 5   ITLLGKLEIDVKIKAPASKVYEIFQERPYLLTKISPENIQSSELLKGEWGKVGSIIYWTY 64
           ++L GKLE DV+I+APASK +EIF +RP+ ++ +S + IQ  E+ +GEWG+VGSI+YW Y
Sbjct: 1   MSLFGKLETDVEIEAPASKFHEIFHQRPHHISNVSGDKIQGCEIHEGEWGQVGSIVYWNY 60

Query: 65  TLGGKTSVVKHIVEAVDEEKNLITFTWIEGTLSEYYKKFQVTVQCIPKHMGSVVRWITEF 124
              GK+ V K I+EAVDEE N+I+F  IEG L E YK F++T+ C+P+  GSV+ W  E+
Sbjct: 61  VHDGKSCVAKEIIEAVDEENNMISFKVIEGDLLEEYKSFKLTIHCLPRDKGSVIHWTLEY 120

Query: 125 EILRVEIPYLCNLPQF 141
           E L  E+P   +L QF
Sbjct: 121 EKLHDEVPDSHSLLQF 136

[Close]

BLAST of Lag0021556.1 vs. NCBI nr
Match: XP_022972261.1 (MLP-like protein 31 [Cucurbita maxima])

HSP 1 Score: 151.8 bits (382), Expect = 4.5e-33
Identity = 72/128 (56.25%), Postives = 91/128 (71.09%), Query Frame = 0


Query: 5   ITLLGKLEIDVKIKAPASKVYEIFQERPYLLTKISPENIQSSELLKGEWGKVGSIIYWTY 64
           ++L GKLE DVKIK PASK +E+F  +P+ ++K   + IQ  +L +GEWGKVGSIIYW Y
Sbjct: 1   MSLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNY 60

Query: 65  TLGGKTSVVKHIVEAVDEEKNLITFTWIEGTLSEYYKKFQVTVQCIPKHMGSVVRWITEF 124
             GGK SV K +VEAVDEE N I F  +EG L + YK F++T+QCIPK  GSVV WI E+
Sbjct: 61  CHGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPKDKGSVVHWIFEY 120

Query: 125 EILRVEIP 133
           E    +IP
Sbjct: 121 EKRHEKIP 128

[Close]

BLAST of Lag0021556.1 vs. NCBI nr
Match: XP_023538921.1 (MLP-like protein 34 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 151.4 bits (381), Expect = 5.9e-33
Identity = 69/136 (50.74%), Postives = 97/136 (71.32%), Query Frame = 0


Query: 5   ITLLGKLEIDVKIKAPASKVYEIFQERPYLLTKISPENIQSSELLKGEWGKVGSIIYWTY 64
           ++L GKLE DV+I+APASK +EIF +RP+ ++ +S + IQ  E+ +GEWG+VGS++YW Y
Sbjct: 1   MSLFGKLETDVEIEAPASKFHEIFHQRPHHISNVSGDKIQGCEIHEGEWGQVGSVVYWNY 60

Query: 65  TLGGKTSVVKHIVEAVDEEKNLITFTWIEGTLSEYYKKFQVTVQCIPKHMGSVVRWITEF 124
              GK  V K I+EAVDEE N+I+F  IEG L E YK F++T+ C+P+  GSV+ W  E+
Sbjct: 61  VHDGKPCVAKEIIEAVDEENNMISFKVIEGDLLEEYKSFKLTIHCLPRDKGSVIHWTLEY 120

Query: 125 EILRVEIPYLCNLPQF 141
           E L  E+P   +L QF
Sbjct: 121 EKLHDEVPDSHSLLQF 136

[Close]

BLAST of Lag0021556.1 vs. NCBI nr
Match: XP_022972260.1 (MLP-like protein 43 [Cucurbita maxima])

HSP 1 Score: 150.6 bits (379), Expect = 1.0e-32
Identity = 71/137 (51.82%), Postives = 98/137 (71.53%), Query Frame = 0


Query: 5   ITLLGKLEIDVKIKAPASKVYEIFQERPYLLTKISPENIQSSELLKGEWGKVGSIIYWTY 64
           +TL GKLE DV+I+APASK +EIF +RP+ ++ +S + IQ  E+ +GEWGKVGSI++W Y
Sbjct: 1   MTLFGKLETDVEIEAPASKFHEIFHQRPHHISNVSSDKIQGCEIHEGEWGKVGSIVHWNY 60

Query: 65  TLGGKTSVVKHIVEAVDEEKNLITFTWIEGTLSEYYKKFQVTVQCIPKH-MGSVVRWITE 124
              GK+ V K I+EAVDEE N+I+F  +EG L E YK F++T+ C+PK   GSV+ W  E
Sbjct: 61  VHDGKSCVAKEIIEAVDEENNMISFKVVEGDLLEAYKSFKLTIHCLPKEDKGSVIHWTLE 120

Query: 125 FEILRVEIPYLCNLPQF 141
           +E L  E+P   +L QF
Sbjct: 121 YEKLHDEVPDSHSLLQF 137

[Close]

BLAST of Lag0021556.1 vs. ExPASy Swiss-Prot
Match: Q9SSK5 (MLP-like protein 43 OS=Arabidopsis thaliana OX=3702 GN=MLP43 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.0e-28
Identity = 65/142 (45.77%), Postives = 90/142 (63.38%), Query Frame = 0


Query: 1   MSTTITLLGKLEIDVKIKAPASKVYEIFQERPYLLTKISPENIQSSELLKGEWGKVGSII 60
           M+   +L+GKLE +V+IKA A K + +F ERP+ ++K +P+ I   EL +G+WGKVGSI+
Sbjct: 1   MAEASSLVGKLETEVEIKASAKKFHHMFTERPHHVSKATPDKIHGCELHEGDWGKVGSIV 60

Query: 61  YWTYTLGGKTSVVKHIVEAVDEEKNLITFTWIEGTLSEYYKKFQVTVQCIPK--HMGSVV 120
            W Y   GK +V K+ +EAVD EKNLITF  +EG L   YK F  T+Q  PK    GS+ 
Sbjct: 61  IWKYVHDGKLTVGKNKIEAVDPEKNLITFKVLEGDLMNEYKSFAFTLQVTPKQGESGSIA 120

Query: 121 RWITEFEILRVEIPYLCNLPQF 141
            W  E+E +  E+ +   L QF
Sbjct: 121 HWHLEYEKISEEVAHPETLLQF 142

[Close]

BLAST of Lag0021556.1 vs. ExPASy Swiss-Prot
Match: Q9SSK7 (MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.5e-28
Identity = 63/132 (47.73%), Postives = 86/132 (65.15%), Query Frame = 0


Query: 11  LEIDVKIKAPASKVYEIFQERPYLLTKISPENIQSSELLKGEWGKVGSIIYWTYTLGGKT 70
           LE +V+IKA A K + +F  +P+ ++K +P NIQS +L +G+WG VGSI++W Y   G+ 
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNYVHDGEA 228

Query: 71  SVVKHIVEAVDEEKNLITFTWIEGTLSEYYKKFQVTVQCIPKH--MGSVVRWITEFEILR 130
            V K  +EAVD EKNLITF  IEG L + YK F +T+Q  PKH   GSVV W  E+E + 
Sbjct: 229 KVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSGSVVHWHFEYEKIN 288

Query: 131 VEIPYLCNLPQF 141
            E+ +   L QF
Sbjct: 289 EEVAHPETLLQF 300

[Close]

BLAST of Lag0021556.1 vs. ExPASy Swiss-Prot
Match: Q9SSK9 (MLP-like protein 28 OS=Arabidopsis thaliana OX=3702 GN=MLP28 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 5.9e-28
Identity = 63/137 (45.99%), Postives = 89/137 (64.96%), Query Frame = 0


Query: 6   TLLGKLEIDVKIKAPASKVYEIFQERPYLLTKISPENIQSSELLKGEWGKVGSIIYWTYT 65
           +L+GKLE DV+IKA A K + +F  +P+ ++K SP NIQ  +L +G+WG VGSI++W Y 
Sbjct: 21  SLVGKLETDVEIKASADKFHHMFAGKPHHVSKASPGNIQGCDLHEGDWGTVGSIVFWNYV 80

Query: 66  LGGKTSVVKHIVEAVDEEKNLITFTWIEGTLSEYYKKFQVTVQCIPK--HMGSVVRWITE 125
             G+  V K  +EAV+ +KNLITF  IEG L + YK F +T+Q  PK    GS+V W  E
Sbjct: 81  HDGEAKVAKERIEAVEPDKNLITFRVIEGDLMKEYKSFLLTIQVTPKPGGPGSIVHWHLE 140

Query: 126 FEILRVEIPYLCNLPQF 141
           +E +  E+ +   L QF
Sbjct: 141 YEKISEEVAHPETLLQF 157

[Close]

BLAST of Lag0021556.1 vs. ExPASy Swiss-Prot
Match: Q941R6 (MLP-like protein 31 OS=Arabidopsis thaliana OX=3702 GN=MLP31 PE=2 SV=2)

HSP 1 Score: 124.0 bits (310), Expect = 1.3e-27
Identity = 61/122 (50.00%), Postives = 81/122 (66.39%), Query Frame = 0


Query: 6   TLLGKLEIDVKIKAPASKVYEIFQERPYLLTKISPENIQSSELLKGEWGKVGSIIYWTYT 65
           +L GKLE D++IKA A K + +F  RP+ ++K +P  IQ  EL +G+WGKVGSI++W Y 
Sbjct: 19  SLCGKLETDIEIKASAGKFHHMFAGRPHHVSKATPGKIQGCELHEGDWGKVGSIVFWNYV 78

Query: 66  LGGKTSVVKHIVEAVDEEKNLITFTWIEGTLSEYYKKFQVTVQCIPKH--MGSVVRWITE 125
             G+  V K  +EAV+ EKNLITF  IEG L + YK F +T+Q  PK    GSVV W  E
Sbjct: 79  HDGEAKVAKERIEAVEPEKNLITFRVIEGDLLKEYKSFVITIQVTPKRGGPGSVVHWHVE 138

[Close]

BLAST of Lag0021556.1 vs. ExPASy Swiss-Prot
Match: P85524 (Kirola OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.7e-22
Identity = 54/125 (43.20%), Postives = 77/125 (61.60%), Query Frame = 0


Query: 7   LLGKLEIDVKIKAPASKVYEIFQERPYLLTKISPENIQSSELLKGEWGKVGSIIYWTYTL 66
           L GK+   V+I +     YEIF+ R YL++++SP NIQ  +LL+G WG VGS+I++ YT+
Sbjct: 3   LSGKMVKQVEILSDGIVFYEIFRYRLYLISEMSPVNIQGVDLLEGNWGTVGSVIFFKYTI 62

Query: 67  GGKTSVVKHIVEAVDEEKNLITFTWIEGTLSEYYKKFQVTVQCIPKHMGSVVRWITEFEI 126
            GK    K IVEA+DEE   +TF  +EG L E YK F + VQ   K   + V W   +E 
Sbjct: 63  DGKEKTAKDIVEAIDEETKSVTFKIVEGDLMELYKTFIIIVQVDTKGEHNSVTWTFHYEK 122

Query: 127 LRVEI 132
           L+ ++
Sbjct: 123 LKEDV 127

The following BLAST results are available for this feature:

BLAST of Lag0021556.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022944436.1	2.0e-33	52.21	MLP-like protein 34 [Cucurbita moschata]	[more]
KAG6597607.1	2.6e-33	51.47	MLP-like protein 31, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022972261.1	4.5e-33	56.25	MLP-like protein 31 [Cucurbita maxima]	[more]
XP_023538921.1	5.9e-33	50.74	MLP-like protein 34 [Cucurbita pepo subsp. pepo]	[more]
XP_022972260.1	1.0e-32	51.82	MLP-like protein 43 [Cucurbita maxima]	[more]

BLAST of Lag0021556.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SSK5	2.0e-28	45.77	MLP-like protein 43 OS=Arabidopsis thaliana OX=3702 GN=MLP43 PE=1 SV=1	[more]
Q9SSK7	4.5e-28	47.73	MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1	[more]
Q9SSK9	5.9e-28	45.99	MLP-like protein 28 OS=Arabidopsis thaliana OX=3702 GN=MLP28 PE=1 SV=1	[more]
Q941R6	1.3e-27	50.00	MLP-like protein 31 OS=Arabidopsis thaliana OX=3702 GN=MLP31 PE=2 SV=2	[more]
P85524	1.7e-22	43.20	Kirola OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000916	Bet v I/Major latex protein	SMART	SM01037	Bet_v_1_2	coord: 6..140 e-value: 8.8E-34 score: 128.2
IPR000916	Bet v I/Major latex protein	PFAM	PF00407	Bet_v_1	coord: 8..126 e-value: 1.4E-35 score: 122.4
IPR023393	START-like domain superfamily	GENE3D	3.30.530.20		coord: 1..136 e-value: 1.1E-41 score: 144.4
None	No IPR available	PANTHER	PTHR31907	MLP-LIKE PROTEIN 423	coord: 6..134
None	No IPR available	PANTHER	PTHR31907:SF4	MLP-LIKE PROTEIN 34-RELATED	coord: 6..134
None	No IPR available	CDD	cd07816	Bet_v1-like	coord: 9..127 e-value: 3.5725E-33 score: 111.898
None	No IPR available	SUPERFAMILY	55961	Bet v1-like	coord: 9..127

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0021556	Lag0021556	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0021556.1.exon2	Lag0021556.1.exon2	exon
Lag0021556.1.exon1	Lag0021556.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0021556.1	cds.Lag0021556.1_2	CDS
cds.Lag0021556.1	cds.Lag0021556.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0021556.1	Lag0021556.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response