Lag0006098.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0006098.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
Location	chr6: 37276944 .. 37277889 (-)
Sequence length	228
RNA-Seq Expression	Lag0006098.1
Synteny	Lag0006098.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCCCCCGCCGTTCAGCCCCGTTTTCTTCGCCGCGCAGCCTCTCACGCCCCCCGTTTTCTTCTTCGTGCAGGTAACACGAGAAGACGCACGCCCCCCGTTTTCTTCTTCGCCTAGGCAACAGGAGTTGTCTCTCCACGCGTCATGCGACCAACCACTGCAAACGTCATCGCCACGCGAATTCCAGCCCTCCAACGAAGCGTTTCGGCTTTTTTTTTTTTTTTTGTTAAGTTTAGCCTCGACCCACTTGATTTCTTCATTTTTCAGCTTATGCCCATGTATTTTGATTTATGTCAGGTTGTAATTTACTCACTTGCTAGAACTGCATCTCAGCAAGGATGAGGGAAGGTTGGCAAGTGGAAAATAAATTTTATGCCAACAAAAAAGTATGAATTGTTCTTTTCTTTCTTCGTTCTCTGAGTTTGTTTTTCTAAAAAATCGGAGTTGCTCCATAAGTTAACAGAATTTTTAACTCAGTTCTAGCAGATTCCTAGGTGCTAGAGTGAAATATTGTGCACAATATGTTATATTGGACAAACTTCAAGTGCACAAAAGTGAATGACTTGTTCTCTTACTCTCAACAATTAGAGTTTTTCCCCTTAATATCTTTTTCTCTTTTTGGACTTTTGTTTCTCTGATCAGGCATCTTATGTGGTGGATTGATTTCTTTCTTTATTGCCCGCGTGTAATATTTCATTTATTTTCATAAAAGTTTGATTTTTCATTTAAAACTGAAAAACATACAAACAGAGGGGTAAAGAGTTTTTTAATGCTTATCGACATGTTTGTAGGTGGGAAAATCCATTGATGGGGTGGACTTCTACTGGGGACCCTTATGCCAATGTAGGGGATTCTACACTGTCTTTTGATAGTGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGATGGGAATATGTGGTAATGCTTCGTTCAACCATTTAG

mRNA sequence

ATGCCCCCCCGCCGTTCAGCCCCGTTTTCTTCGCCGCGCAGCCTCTCACGCCCCCCGTTTTCTTCTTCGTGCAGGTGGGAAAATCCATTGATGGGGTGGACTTCTACTGGGGACCCTTATGCCAATGTAGGGGATTCTACACTGTCTTTTGATAGTGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGATGGGAATATGTGGTAATGCTTCGTTCAACCATTTAG

Coding sequence (CDS)

ATGCCCCCCCGCCGTTCAGCCCCGTTTTCTTCGCCGCGCAGCCTCTCACGCCCCCCGTTTTCTTCTTCGTGCAGGTGGGAAAATCCATTGATGGGGTGGACTTCTACTGGGGACCCTTATGCCAATGTAGGGGATTCTACACTGTCTTTTGATAGTGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGATGGGAATATGTGGTAATGCTTCGTTCAACCATTTAG

Protein sequence

MPPRRSAPFSSPRSLSRPPFSSSCRWENPLMGWTSTGDPYANVGDSTLSFDSEEAAKAFAEKHGWEYVVMLRSTI

Homology

[Close]

BLAST of Lag0006098.1 vs. NCBI nr
Match: XP_038881462.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 103.6 bits (257), Expect = 7.6e-19
Identity = 47/55 (85.45%), Postives = 49/55 (89.09%), Query Frame = 0


Query: 20  FSSSCRWENPLMGWTSTGDPYANVGDSTLSFDSEEAAKAFAEKHGWEYVVMLRST 75
           F S+ +WENPLMGWTSTGDPYANVGDS LSFDSEEAAKAFAEKHGWEYVV  R T
Sbjct: 78  FMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVQKRHT 132

[Close]

BLAST of Lag0006098.1 vs. NCBI nr
Match: XP_022962977.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita moschata] >XP_022962978.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita moschata] >XP_023003205.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita maxima] >XP_023517797.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita pepo subsp. pepo] >KAG6594972.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 103.2 bits (256), Expect = 9.9e-19
Identity = 47/55 (85.45%), Postives = 49/55 (89.09%), Query Frame = 0


Query: 20  FSSSCRWENPLMGWTSTGDPYANVGDSTLSFDSEEAAKAFAEKHGWEYVVMLRST 75
           F S+ +WENPLMGWTSTGDPYANVGDS LSFDSEEAAKAFAEKHGWEYVV  R T
Sbjct: 78  FMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHT 132

[Close]

BLAST of Lag0006098.1 vs. NCBI nr
Match: KAG7026931.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 103.2 bits (256), Expect = 9.9e-19
Identity = 47/55 (85.45%), Postives = 49/55 (89.09%), Query Frame = 0


Query: 20  FSSSCRWENPLMGWTSTGDPYANVGDSTLSFDSEEAAKAFAEKHGWEYVVMLRST 75
           F S+ +WENPLMGWTSTGDPYANVGDS LSFDSEEAAKAFAEKHGWEYVV  R T
Sbjct: 78  FMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHT 132

[Close]

BLAST of Lag0006098.1 vs. NCBI nr
Match: XP_008440406.1 (PREDICTED: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Cucumis melo] >KAA0036408.1 NADH dehydrogenase (ubiquinone) iron-sulfur protein 4 [Cucumis melo var. makuwa] >TYK12804.1 NADH dehydrogenase (ubiquinone) iron-sulfur protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 102.8 bits (255), Expect = 1.3e-18
Identity = 47/55 (85.45%), Postives = 49/55 (89.09%), Query Frame = 0


Query: 20  FSSSCRWENPLMGWTSTGDPYANVGDSTLSFDSEEAAKAFAEKHGWEYVVMLRST 75
           F S+ +WENPLMGWTSTGDPYANVGDS LSFDSEEAAKAFAEKHGWEYVV  R T
Sbjct: 78  FVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHT 132

[Close]

BLAST of Lag0006098.1 vs. NCBI nr
Match: XP_004141860.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Cucumis sativus] >KGN48618.1 hypothetical protein Csa_004287 [Cucumis sativus])

HSP 1 Score: 102.8 bits (255), Expect = 1.3e-18
Identity = 47/55 (85.45%), Postives = 49/55 (89.09%), Query Frame = 0


Query: 20  FSSSCRWENPLMGWTSTGDPYANVGDSTLSFDSEEAAKAFAEKHGWEYVVMLRST 75
           F S+ +WENPLMGWTSTGDPYANVGDS LSFDSEEAAKAFAEKHGWEYVV  R T
Sbjct: 78  FVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHT 132

[Close]

BLAST of Lag0006098.1 vs. ExPASy Swiss-Prot
Match: Q9FJW4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 2.3e-18
Identity = 46/74 (62.16%), Postives = 55/74 (74.32%), Query Frame = 0


Query: 3   PRRSAPFSSPRSLS--RPPFSSSCRWENPLMGWTSTGDPYANVGDSTLSFDSEEAAKAFA 62
           P R+A  S    L   +  F S+ +WENPLMGWTSTGDPYANVGDS L+FDSEEAAK+FA
Sbjct: 59  PARTATQSGSGKLGKWKINFVSTLKWENPLMGWTSTGDPYANVGDSALAFDSEEAAKSFA 118

Query: 63  EKHGWEYVVMLRST 75
           E+HGW+Y V   +T
Sbjct: 119 ERHGWDYKVKKPNT 132

[Close]

BLAST of Lag0006098.1 vs. ExPASy Swiss-Prot
Match: Q5XIF3 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Rattus norvegicus OX=10116 GN=Ndufs4 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.5e-09
Identity = 29/50 (58.00%), Postives = 35/50 (70.00%), Query Frame = 0


Query: 20  FSSSCRWENPLMGWTSTGDPYANVGDSTLSFDSEEAAKAFAEKHGWEYVV 70
           F +  RWENPLMGW ST DP +N+    L+F ++E A AFAEKHGW Y V
Sbjct: 101 FDTRERWENPLMGWASTADPLSNM---VLTFSAKEDAVAFAEKHGWSYDV 147

[Close]

BLAST of Lag0006098.1 vs. ExPASy Swiss-Prot
Match: Q02375 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Bos taurus OX=9913 GN=NDUFS4 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 9.6e-09
Identity = 28/50 (56.00%), Postives = 35/50 (70.00%), Query Frame = 0


Query: 20  FSSSCRWENPLMGWTSTGDPYANVGDSTLSFDSEEAAKAFAEKHGWEYVV 70
           F +  RWENPLMGW ST DP +N+    L+F ++E A AFAEK+GW Y V
Sbjct: 101 FDTRERWENPLMGWASTADPLSNL---VLTFSTKEDAVAFAEKNGWSYDV 147

[Close]

BLAST of Lag0006098.1 vs. ExPASy Swiss-Prot
Match: Q9CXZ1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Mus musculus OX=10090 GN=Ndufs4 PE=1 SV=3)

HSP 1 Score: 60.1 bits (144), Expect = 1.3e-08
Identity = 28/50 (56.00%), Postives = 35/50 (70.00%), Query Frame = 0


Query: 20  FSSSCRWENPLMGWTSTGDPYANVGDSTLSFDSEEAAKAFAEKHGWEYVV 70
           F +  RWENPLMGW ST DP +N+    L+F ++E A AFAEK+GW Y V
Sbjct: 101 FDTRERWENPLMGWASTADPLSNM---VLTFSAKEDAIAFAEKNGWSYDV 147

[Close]

BLAST of Lag0006098.1 vs. ExPASy Swiss-Prot
Match: Q66XS7 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gekko japonicus OX=146911 GN=NDUFS4 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 2.1e-08
Identity = 27/50 (54.00%), Postives = 36/50 (72.00%), Query Frame = 0


Query: 20  FSSSCRWENPLMGWTSTGDPYANVGDSTLSFDSEEAAKAFAEKHGWEYVV 70
           F +  RWENPLMGW+ST DP +N+    L+F ++E A AFAEK+GW + V
Sbjct: 101 FDTRERWENPLMGWSSTADPLSNL---VLTFSTKEDAVAFAEKNGWSFDV 147

The following BLAST results are available for this feature:

BLAST of Lag0006098.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881462.1	7.6e-19	85.45	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Benincasa ...	[more]
XP_022962977.1	9.9e-19	85.45	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucur...	[more]
KAG7026931.1	9.9e-19	85.45	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Cucurbita ...	[more]
XP_008440406.1	1.3e-18	85.45	PREDICTED: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial ...	[more]
XP_004141860.1	1.3e-18	85.45	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Cucumis sa...	[more]

BLAST of Lag0006098.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJW4	2.3e-18	62.16	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Arabidop...	[more]
Q5XIF3	1.5e-09	58.00	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Rattus n...	[more]
Q02375	9.6e-09	56.00	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Bos taur...	[more]
Q9CXZ1	1.3e-08	56.00	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Mus musc...	[more]
Q66XS7	2.1e-08	54.00	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gekko ja...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006885	NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial	PFAM	PF04800	ETC_C1_NDUFA4	coord: 23..70 e-value: 1.4E-16 score: 60.5
IPR006885	NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial	PANTHER	PTHR12219	NADH-UBIQUINONE OXIDOREDUCTASE	coord: 20..70
IPR038532	NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily	GENE3D	3.30.160.190	atu1810 like domain	coord: 15..70 e-value: 1.6E-20 score: 74.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR12219:SF25	NADH DEHYDROGENASE UBIQUINONE FE-S PROTEIN-RELATED	coord: 20..70

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0006098	Lag0006098	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0006098.1.exon2	Lag0006098.1.exon2	exon
Lag0006098.1.exon1	Lag0006098.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0006098.1	cds.Lag0006098.1_2	CDS
cds.Lag0006098.1	cds.Lag0006098.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0006098.1	Lag0006098.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0005743	mitochondrial inner membrane
cellular_component	GO:0070469	respirasome
molecular_function	GO:0016651	oxidoreductase activity, acting on NAD(P)H