Lag0003082.1 (mRNA) Sponge gourd (AG‐4) v1

Overview
NameLag0003082.1
TypemRNA
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionESCRT-related protein CHMP1B
Locationchr4: 47908470 .. 47909081 (-)
Sequence length612
RNA-Seq ExpressionLag0003082.1
SyntenyLag0003082.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAATACCGAGAAATTGTTGAATCAAATCATGGAATTGAAGTTCACTTCGAAGTCGCTGCAGCGCCAAGCCAGGAAGTGCGAGAAGGAGGAGAAAGCCGAGAAGCTCAAGATCAAGAAGGCCATGGAGAAGGGAAACGTTGACGGAGCCCGAATCTACGCGGAGAACGCGATCCGTAAGCGAACGGAACAGATGAACTATCTCCGCCTTTCTTCGAGGCTTGATGCTGTCGTGGCTCGGCTCGATACACAGGCTAAGATGACCACGATCAACAAGTCCATGGCATCGATCGTGAAGTCATTGGAATCTTCTTTGGCGACTGGGAATTTGCAGAAGATGTCTGAGACTATGGACCAGTTTGAGAAGCAGTTTGTGAATATGGAGGTTCAGGCCGAGTTCATGGAAAATGCAATGGCCGGATCGACGTCGCTCTCGACTCCGGAGGGAGAAGTCAACAGCTTGATGCAGCAGGTTGCGGATGATTACGGTCTGGAAGTCTCCGTCGGGCTACCGCAGCCGGCGTCGCATGCAGTGCCGACCAAGGAGACCGAGAAAGTTGACGAGGACGACTTATCGAGGCGCCTTGCAGAGCTTAAGGCCAGAGGTTAA

mRNA sequence

ATGGGGAATACCGAGAAATTGTTGAATCAAATCATGGAATTGAAGTTCACTTCGAAGTCGCTGCAGCGCCAAGCCAGGAAGTGCGAGAAGGAGGAGAAAGCCGAGAAGCTCAAGATCAAGAAGGCCATGGAGAAGGGAAACGTTGACGGAGCCCGAATCTACGCGGAGAACGCGATCCGTAAGCGAACGGAACAGATGAACTATCTCCGCCTTTCTTCGAGGCTTGATGCTGTCGTGGCTCGGCTCGATACACAGGCTAAGATGACCACGATCAACAAGTCCATGGCATCGATCGTGAAGTCATTGGAATCTTCTTTGGCGACTGGGAATTTGCAGAAGATGTCTGAGACTATGGACCAGTTTGAGAAGCAGTTTGTGAATATGGAGGTTCAGGCCGAGTTCATGGAAAATGCAATGGCCGGATCGACGTCGCTCTCGACTCCGGAGGGAGAAGTCAACAGCTTGATGCAGCAGGTTGCGGATGATTACGGTCTGGAAGTCTCCGTCGGGCTACCGCAGCCGGCGTCGCATGCAGTGCCGACCAAGGAGACCGAGAAAGTTGACGAGGACGACTTATCGAGGCGCCTTGCAGAGCTTAAGGCCAGAGGTTAA

Coding sequence (CDS)

ATGGGGAATACCGAGAAATTGTTGAATCAAATCATGGAATTGAAGTTCACTTCGAAGTCGCTGCAGCGCCAAGCCAGGAAGTGCGAGAAGGAGGAGAAAGCCGAGAAGCTCAAGATCAAGAAGGCCATGGAGAAGGGAAACGTTGACGGAGCCCGAATCTACGCGGAGAACGCGATCCGTAAGCGAACGGAACAGATGAACTATCTCCGCCTTTCTTCGAGGCTTGATGCTGTCGTGGCTCGGCTCGATACACAGGCTAAGATGACCACGATCAACAAGTCCATGGCATCGATCGTGAAGTCATTGGAATCTTCTTTGGCGACTGGGAATTTGCAGAAGATGTCTGAGACTATGGACCAGTTTGAGAAGCAGTTTGTGAATATGGAGGTTCAGGCCGAGTTCATGGAAAATGCAATGGCCGGATCGACGTCGCTCTCGACTCCGGAGGGAGAAGTCAACAGCTTGATGCAGCAGGTTGCGGATGATTACGGTCTGGAAGTCTCCGTCGGGCTACCGCAGCCGGCGTCGCATGCAGTGCCGACCAAGGAGACCGAGAAAGTTGACGAGGACGACTTATCGAGGCGCCTTGCAGAGCTTAAGGCCAGAGGTTAA

Protein sequence

MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIRKRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQFEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVPTKETEKVDEDDLSRRLAELKARG
Homology
BLAST of Lag0003082.1 vs. NCBI nr
Match: XP_022144640.1 (ESCRT-related protein CHMP1B [Momordica charantia])

HSP 1 Score: 368.6 bits (945), Expect = 3.4e-98
Identity = 201/203 (99.01%), Postives = 203/203 (100.00%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR
Sbjct: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ
Sbjct: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP
Sbjct: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           T+ETEKVDEDDL+RRLAELKARG
Sbjct: 181 TRETEKVDEDDLTRRLAELKARG 203

BLAST of Lag0003082.1 vs. NCBI nr
Match: XP_022964312.1 (ESCRT-related protein CHMP1B [Cucurbita moschata] >XP_023000394.1 ESCRT-related protein CHMP1B [Cucurbita maxima] >XP_023513530.1 ESCRT-related protein CHMP1B [Cucurbita pepo subsp. pepo] >KAG6593503.1 ESCRT-related protein CHMP1A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025850.1 ESCRT-related protein CHMP1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 368.2 bits (944), Expect = 4.5e-98
Identity = 201/203 (99.01%), Postives = 202/203 (99.51%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNT+KLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR
Sbjct: 1   MGNTDKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSL TGNLQKMSETMDQ
Sbjct: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLTTGNLQKMSETMDQ 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP
Sbjct: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           TKETEKVDEDDLSRRLAELKARG
Sbjct: 181 TKETEKVDEDDLSRRLAELKARG 203

BLAST of Lag0003082.1 vs. NCBI nr
Match: XP_004141672.1 (ESCRT-related protein CHMP1B [Cucumis sativus] >XP_008462374.1 PREDICTED: ESCRT-related protein CHMP1B [Cucumis melo] >KAA0059449.1 ESCRT-related protein CHMP1B [Cucumis melo var. makuwa] >KGN45545.1 hypothetical protein Csa_015849 [Cucumis sativus] >TYK03875.1 ESCRT-related protein CHMP1B [Cucumis melo var. makuwa])

HSP 1 Score: 367.5 bits (942), Expect = 7.6e-98
Identity = 200/203 (98.52%), Postives = 203/203 (100.00%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEK+EKLKIKKAMEKGNVDGARIYAENAIR
Sbjct: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKSEKLKIKKAMEKGNVDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLES+LATGNLQKMSETMDQ
Sbjct: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESTLATGNLQKMSETMDQ 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP
Sbjct: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           TKETEKVDEDDL+RRLAELKARG
Sbjct: 181 TKETEKVDEDDLTRRLAELKARG 203

BLAST of Lag0003082.1 vs. NCBI nr
Match: XP_038896979.1 (ESCRT-related protein CHMP1B [Benincasa hispida])

HSP 1 Score: 366.3 bits (939), Expect = 1.7e-97
Identity = 200/203 (98.52%), Postives = 202/203 (99.51%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEK+EKLKIKKAMEKGNVDGARIYAENAIR
Sbjct: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKSEKLKIKKAMEKGNVDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ
Sbjct: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVN LMQQVADDYGLEVSVGLPQPASHAVP
Sbjct: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNILMQQVADDYGLEVSVGLPQPASHAVP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           TKETEKVDEDDL+RRLAELKARG
Sbjct: 181 TKETEKVDEDDLTRRLAELKARG 203

BLAST of Lag0003082.1 vs. NCBI nr
Match: XP_022954345.1 (ESCRT-related protein CHMP1B-like [Cucurbita moschata] >XP_023549216.1 ESCRT-related protein CHMP1B-like [Cucurbita pepo subsp. pepo] >KAG6575490.1 ESCRT-related protein CHMP1A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014033.1 ESCRT-related protein CHMP1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 365.2 bits (936), Expect = 3.8e-97
Identity = 199/203 (98.03%), Postives = 202/203 (99.51%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR
Sbjct: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KRTEQMNYLRLSSRLDAVVARLDTQAKM+TINKSMASIVKSLESSLATGNLQKMSE MDQ
Sbjct: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMSTINKSMASIVKSLESSLATGNLQKMSENMDQ 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHA+P
Sbjct: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAMP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           TKETEKVDEDDL+RRLAELKARG
Sbjct: 181 TKETEKVDEDDLTRRLAELKARG 203

BLAST of Lag0003082.1 vs. ExPASy Swiss-Prot
Match: Q9SSM4 (ESCRT-related protein CHMP1B OS=Arabidopsis thaliana OX=3702 GN=CHMP1B PE=1 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 4.1e-94
Identity = 185/203 (91.13%), Postives = 196/203 (96.55%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNT+KL+NQI +LKFTSKSLQRQ+RKCEKEEKAEKLK+KKA+EKGN+DGARIYAENAIR
Sbjct: 1   MGNTDKLMNQIFDLKFTSKSLQRQSRKCEKEEKAEKLKVKKAIEKGNMDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KR+EQMNYLRL+SRLDAVVARLDTQAKMTTI KSM +IVKSLESSLATGNLQKMSETMD 
Sbjct: 61  KRSEQMNYLRLASRLDAVVARLDTQAKMTTITKSMTNIVKSLESSLATGNLQKMSETMDS 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPA HA+P
Sbjct: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPAGHAIP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           TK  EKVDEDDLSRRLAELKARG
Sbjct: 181 TKTEEKVDEDDLSRRLAELKARG 203

BLAST of Lag0003082.1 vs. ExPASy Swiss-Prot
Match: Q8LE58 (ESCRT-related protein CHMP1A OS=Arabidopsis thaliana OX=3702 GN=CHMP1A PE=1 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 2.9e-92
Identity = 181/203 (89.16%), Postives = 194/203 (95.57%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNT+KL+NQI ELKFTSKSLQRQARKCEKEE++EKLK+KKA+EKGN+DGARIYAENAIR
Sbjct: 1   MGNTDKLMNQIFELKFTSKSLQRQARKCEKEERSEKLKVKKAIEKGNMDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KR+EQMNYLRLSSRLDAVVARLDTQAKM TI KSM +IVKSLESSL TGNLQKMSETMD 
Sbjct: 61  KRSEQMNYLRLSSRLDAVVARLDTQAKMATITKSMTNIVKSLESSLTTGNLQKMSETMDS 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFM+NAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPA HA+P
Sbjct: 121 FEKQFVNMEVQAEFMDNAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPAGHAIP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           TK  EKV+EDDL+RRLAELKARG
Sbjct: 181 TKTEEKVEEDDLTRRLAELKARG 203

BLAST of Lag0003082.1 vs. ExPASy Swiss-Prot
Match: Q84VG1 (ESCRT-related protein CHMP1 OS=Oryza sativa subsp. japonica OX=39947 GN=CHMP1 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 2.0e-85
Identity = 176/205 (85.85%), Postives = 189/205 (92.20%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGN EKL+ QI +LKFTSKSLQRQARKCEKEEK +KLK+KKA+EKGN+DGARIYAENAIR
Sbjct: 1   MGNPEKLMTQIFDLKFTSKSLQRQARKCEKEEKEQKLKVKKAIEKGNMDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KRTE MNYLRL+SRLDAVVARLDTQAKM  I KSMA+IVKSL+S+LATGNLQKMSETMD 
Sbjct: 61  KRTEHMNYLRLASRLDAVVARLDTQAKMQVIGKSMANIVKSLDSALATGNLQKMSETMDN 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FE+QFVNMEVQAEFME AMAGSTSLSTPE EVNSLMQQVADDYGLEVSVGLPQ A+HA+P
Sbjct: 121 FERQFVNMEVQAEFMEGAMAGSTSLSTPETEVNSLMQQVADDYGLEVSVGLPQAAAHAIP 180

Query: 181 -TKETEK-VDEDDLSRRLAELKARG 204
             KE EK VDEDDLSRRLAELKARG
Sbjct: 181 AAKEKEKAVDEDDLSRRLAELKARG 205

BLAST of Lag0003082.1 vs. ExPASy Swiss-Prot
Match: O96552 (Charged multivesicular body protein 1 OS=Dictyostelium discoideum OX=44689 GN=chmp1 PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 6.6e-44
Identity = 92/194 (47.42%), Postives = 152/194 (78.35%), Query Frame = 0

Query: 9   NQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIRKRTEQMNY 68
           NQ+ +LKFTSK L++Q++K E+ EKA+K+K+KKA+E+GN+DGARIYA+NAIR++ + +NY
Sbjct: 3   NQLFQLKFTSKQLEKQSKKSEQSEKAQKIKLKKAIEQGNMDGARIYAQNAIREKNQSLNY 62

Query: 69  LRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQFEKQFVNM 128
           LRL+SR+DAV +R++T  +M ++  SMA+IVKS+E S+   +L+K+++ MDQFE+QF ++
Sbjct: 63  LRLASRIDAVASRVETAIRMKSVTGSMANIVKSMEKSMRNMDLEKITQVMDQFERQFEDL 122

Query: 129 EVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVPTKETEKVD 188
           +VQ+ ++ENAM  +T+LSTP  +V+ L+ QVAD++GL V + +      + P+++ ++ +
Sbjct: 123 DVQSVYVENAMNQTTTLSTPADQVDLLISQVADEHGLNVGMQM-----GSAPSEKVQQGE 182

Query: 189 EDDLSRRLAELKAR 203
            D+L+ RL  LK +
Sbjct: 183 TDELTERLNRLKQK 191

BLAST of Lag0003082.1 vs. ExPASy Swiss-Prot
Match: Q5E994 (Charged multivesicular body protein 1b OS=Bos taurus OX=9913 GN=CHMP1B PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.5e-43
Identity = 97/200 (48.50%), Postives = 150/200 (75.00%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           M N EK    +  LKF +K L R A+KC+KEEKAEK KIKKA++KGN++ ARI+AENAIR
Sbjct: 1   MSNMEK---HLFNLKFAAKELGRSAKKCDKEEKAEKAKIKKAIQKGNMEVARIHAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           ++ + +N+LR+S+R+DAV AR+ T   M  + KSMA +VKS++++L T NL+K+S  MD+
Sbjct: 61  QKNQAVNFLRMSARVDAVAARVQTAVTMGKVTKSMAGVVKSMDATLKTMNLEKISALMDK 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FE QF  ++VQ + ME+ M+ +T+L+TP+G+V+ L+Q++AD+ GL++++ LPQ  + +V 
Sbjct: 121 FEHQFETLDVQTQQMEDTMSSTTTLTTPQGQVDMLLQEMADEAGLDLNMELPQGQTGSVG 180

Query: 181 TKETEKVDEDDLSRRLAELK 201
           T      ++D+LS+RLA L+
Sbjct: 181 T-SVASAEQDELSQRLARLR 196

BLAST of Lag0003082.1 vs. ExPASy TrEMBL
Match: A0A6J1CS80 (ESCRT-related protein CHMP1B OS=Momordica charantia OX=3673 GN=LOC111014278 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.7e-98
Identity = 201/203 (99.01%), Postives = 203/203 (100.00%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR
Sbjct: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ
Sbjct: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP
Sbjct: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           T+ETEKVDEDDL+RRLAELKARG
Sbjct: 181 TRETEKVDEDDLTRRLAELKARG 203

BLAST of Lag0003082.1 vs. ExPASy TrEMBL
Match: A0A6J1KJS6 (ESCRT-related protein CHMP1B OS=Cucurbita maxima OX=3661 GN=LOC111494652 PE=4 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.2e-98
Identity = 201/203 (99.01%), Postives = 202/203 (99.51%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNT+KLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR
Sbjct: 1   MGNTDKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSL TGNLQKMSETMDQ
Sbjct: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLTTGNLQKMSETMDQ 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP
Sbjct: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           TKETEKVDEDDLSRRLAELKARG
Sbjct: 181 TKETEKVDEDDLSRRLAELKARG 203

BLAST of Lag0003082.1 vs. ExPASy TrEMBL
Match: A0A6J1HHH0 (ESCRT-related protein CHMP1B OS=Cucurbita moschata OX=3662 GN=LOC111464364 PE=4 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.2e-98
Identity = 201/203 (99.01%), Postives = 202/203 (99.51%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNT+KLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR
Sbjct: 1   MGNTDKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSL TGNLQKMSETMDQ
Sbjct: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLTTGNLQKMSETMDQ 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP
Sbjct: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           TKETEKVDEDDLSRRLAELKARG
Sbjct: 181 TKETEKVDEDDLSRRLAELKARG 203

BLAST of Lag0003082.1 vs. ExPASy TrEMBL
Match: A0A5A7V0Y1 (ESCRT-related protein CHMP1B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00700 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 3.7e-98
Identity = 200/203 (98.52%), Postives = 203/203 (100.00%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEK+EKLKIKKAMEKGNVDGARIYAENAIR
Sbjct: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKSEKLKIKKAMEKGNVDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLES+LATGNLQKMSETMDQ
Sbjct: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESTLATGNLQKMSETMDQ 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP
Sbjct: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           TKETEKVDEDDL+RRLAELKARG
Sbjct: 181 TKETEKVDEDDLTRRLAELKARG 203

BLAST of Lag0003082.1 vs. ExPASy TrEMBL
Match: A0A0A0K779 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G452000 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 3.7e-98
Identity = 200/203 (98.52%), Postives = 203/203 (100.00%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEK+EKLKIKKAMEKGNVDGARIYAENAIR
Sbjct: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKSEKLKIKKAMEKGNVDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLES+LATGNLQKMSETMDQ
Sbjct: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESTLATGNLQKMSETMDQ 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP
Sbjct: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           TKETEKVDEDDL+RRLAELKARG
Sbjct: 181 TKETEKVDEDDLTRRLAELKARG 203

BLAST of Lag0003082.1 vs. TAIR 10
Match: AT1G73030.1 (SNF7 family protein )

HSP 1 Score: 345.5 bits (885), Expect = 2.9e-95
Identity = 185/203 (91.13%), Postives = 196/203 (96.55%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNT+KL+NQI +LKFTSKSLQRQ+RKCEKEEKAEKLK+KKA+EKGN+DGARIYAENAIR
Sbjct: 1   MGNTDKLMNQIFDLKFTSKSLQRQSRKCEKEEKAEKLKVKKAIEKGNMDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KR+EQMNYLRL+SRLDAVVARLDTQAKMTTI KSM +IVKSLESSLATGNLQKMSETMD 
Sbjct: 61  KRSEQMNYLRLASRLDAVVARLDTQAKMTTITKSMTNIVKSLESSLATGNLQKMSETMDS 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPA HA+P
Sbjct: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPAGHAIP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           TK  EKVDEDDLSRRLAELKARG
Sbjct: 181 TKTEEKVDEDDLSRRLAELKARG 203

BLAST of Lag0003082.1 vs. TAIR 10
Match: AT1G17730.1 (vacuolar protein sorting 46.1 )

HSP 1 Score: 339.3 bits (869), Expect = 2.1e-93
Identity = 181/203 (89.16%), Postives = 194/203 (95.57%), Query Frame = 0

Query: 1   MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIR 60
           MGNT+KL+NQI ELKFTSKSLQRQARKCEKEE++EKLK+KKA+EKGN+DGARIYAENAIR
Sbjct: 1   MGNTDKLMNQIFELKFTSKSLQRQARKCEKEERSEKLKVKKAIEKGNMDGARIYAENAIR 60

Query: 61  KRTEQMNYLRLSSRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQ 120
           KR+EQMNYLRLSSRLDAVVARLDTQAKM TI KSM +IVKSLESSL TGNLQKMSETMD 
Sbjct: 61  KRSEQMNYLRLSSRLDAVVARLDTQAKMATITKSMTNIVKSLESSLTTGNLQKMSETMDS 120

Query: 121 FEKQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAVP 180
           FEKQFVNMEVQAEFM+NAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPA HA+P
Sbjct: 121 FEKQFVNMEVQAEFMDNAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPAGHAIP 180

Query: 181 TKETEKVDEDDLSRRLAELKARG 204
           TK  EKV+EDDL+RRLAELKARG
Sbjct: 181 TKTEEKVEEDDLTRRLAELKARG 203

BLAST of Lag0003082.1 vs. TAIR 10
Match: AT2G06530.1 (SNF7 family protein )

HSP 1 Score: 58.2 bits (139), Expect = 9.2e-09
Identity = 41/161 (25.47%), Postives = 82/161 (50.93%), Query Frame = 0

Query: 19  KSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIRKRTEQMNYLRLSSRLDAV 78
           + ++R+ +  + +EK    +IKK  ++G +   ++ A++ IR R +   + +L S+L  V
Sbjct: 28  REIERERQGLQTQEKKLINEIKKTAKQGQMGAVKVMAKDLIRTRHQIEKFYKLKSQLQGV 87

Query: 79  VARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQFEKQFVNMEVQAEFMENA 138
             R+ T      + ++M  + K++       NL  + + M +FE+Q   ME+ +E M +A
Sbjct: 88  SLRIQTLKSTQAMGEAMKGVTKAMGQMNRQMNLPSLQKIMQEFERQNEKMEMVSEVMGDA 147

Query: 139 MAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQPASHAV 180
           +  +      E E   L+ QV D+ G++++  L    S AV
Sbjct: 148 IDDALEGDEEEEETEDLVSQVLDEIGIDINQELVNAPSGAV 188

BLAST of Lag0003082.1 vs. TAIR 10
Match: AT5G44560.1 (SNF7 family protein )

HSP 1 Score: 52.8 bits (125), Expect = 3.9e-07
Identity = 48/201 (23.88%), Postives = 85/201 (42.29%), Query Frame = 0

Query: 13  ELKFTSKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIRKRTEQMNYLRLS 72
           E+   ++ ++R+    + EEK    +IKK  + GN    +I A   +R R +  N     
Sbjct: 20  EMAVATRGIEREITSLQLEEKRLVAEIKKTAKTGNEAATKILARQLVRLRQQITNLQGSR 79

Query: 73  SRLDAVVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQFEKQFVNMEVQA 132
           +++  V          T+I+  M    K++ +        K ++ +  F+KQ   +++  
Sbjct: 80  AQIRGVTTHTQALYASTSISSGMKGATKAMVAMNKQMAPTKQAKVIKDFQKQSAQLDMTI 139

Query: 133 EFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQ-------------PASHAV 192
           E M  A+  +      E E   L  QV D+ G+ V+  L               PAS A 
Sbjct: 140 EMMSEAIDETLDKDEAEEETEDLTNQVLDEIGVGVASQLSSAPKGRIATKTAAPPASTAA 199

Query: 193 PTKETEKVDEDDLSRRLAELK 201
             K +E  + D+L +RLA L+
Sbjct: 200 TNKNSESSEVDELEKRLASLR 220

BLAST of Lag0003082.1 vs. TAIR 10
Match: AT5G44560.2 (SNF7 family protein )

HSP 1 Score: 51.2 bits (121), Expect = 1.1e-06
Identity = 47/196 (23.98%), Postives = 83/196 (42.35%), Query Frame = 0

Query: 18  SKSLQRQARKCEKEEKAEKLKIKKAMEKGNVDGARIYAENAIRKRTEQMNYLRLSSRLDA 77
           ++ ++R+    + EEK    +IKK  + GN    +I A   +R R +  N     +++  
Sbjct: 5   TRGIEREITSLQLEEKRLVAEIKKTAKTGNEAATKILARQLVRLRQQITNLQGSRAQIRG 64

Query: 78  VVARLDTQAKMTTINKSMASIVKSLESSLATGNLQKMSETMDQFEKQFVNMEVQAEFMEN 137
           V          T+I+  M    K++ +        K ++ +  F+KQ   +++  E M  
Sbjct: 65  VTTHTQALYASTSISSGMKGATKAMVAMNKQMAPTKQAKVIKDFQKQSAQLDMTIEMMSE 124

Query: 138 AMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVGLPQ-------------PASHAVPTKET 197
           A+  +      E E   L  QV D+ G+ V+  L               PAS A   K +
Sbjct: 125 AIDETLDKDEAEEETEDLTNQVLDEIGVGVASQLSSAPKGRIATKTAAPPASTAATNKNS 184

Query: 198 EKVDEDDLSRRLAELK 201
           E  + D+L +RLA L+
Sbjct: 185 ESSEVDELEKRLASLR 200

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144640.13.4e-9899.01ESCRT-related protein CHMP1B [Momordica charantia][more]
XP_022964312.14.5e-9899.01ESCRT-related protein CHMP1B [Cucurbita moschata] >XP_023000394.1 ESCRT-related ... [more]
XP_004141672.17.6e-9898.52ESCRT-related protein CHMP1B [Cucumis sativus] >XP_008462374.1 PREDICTED: ESCRT-... [more]
XP_038896979.11.7e-9798.52ESCRT-related protein CHMP1B [Benincasa hispida][more]
XP_022954345.13.8e-9798.03ESCRT-related protein CHMP1B-like [Cucurbita moschata] >XP_023549216.1 ESCRT-rel... [more]
Match NameE-valueIdentityDescription
Q9SSM44.1e-9491.13ESCRT-related protein CHMP1B OS=Arabidopsis thaliana OX=3702 GN=CHMP1B PE=1 SV=1[more]
Q8LE582.9e-9289.16ESCRT-related protein CHMP1A OS=Arabidopsis thaliana OX=3702 GN=CHMP1A PE=1 SV=1[more]
Q84VG12.0e-8585.85ESCRT-related protein CHMP1 OS=Oryza sativa subsp. japonica OX=39947 GN=CHMP1 PE... [more]
O965526.6e-4447.42Charged multivesicular body protein 1 OS=Dictyostelium discoideum OX=44689 GN=ch... [more]
Q5E9941.5e-4348.50Charged multivesicular body protein 1b OS=Bos taurus OX=9913 GN=CHMP1B PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CS801.7e-9899.01ESCRT-related protein CHMP1B OS=Momordica charantia OX=3673 GN=LOC111014278 PE=4... [more]
A0A6J1KJS62.2e-9899.01ESCRT-related protein CHMP1B OS=Cucurbita maxima OX=3661 GN=LOC111494652 PE=4 SV... [more]
A0A6J1HHH02.2e-9899.01ESCRT-related protein CHMP1B OS=Cucurbita moschata OX=3662 GN=LOC111464364 PE=4 ... [more]
A0A5A7V0Y13.7e-9898.52ESCRT-related protein CHMP1B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A0A0K7793.7e-9898.52Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G452000 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G73030.12.9e-9591.13SNF7 family protein [more]
AT1G17730.12.1e-9389.16vacuolar protein sorting 46.1 [more]
AT2G06530.19.2e-0925.47SNF7 family protein [more]
AT5G44560.13.9e-0723.88SNF7 family protein [more]
AT5G44560.21.1e-0623.98SNF7 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 111..138
NoneNo IPR availableCOILSCoilCoilcoord: 182..202
NoneNo IPR availableCOILSCoilCoilcoord: 11..45
NoneNo IPR availableGENE3D6.10.140.1230coord: 3..173
e-value: 1.9E-38
score: 134.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 171..191
NoneNo IPR availablePANTHERPTHR10476CHARGED MULTIVESICULAR BODY PROTEINcoord: 5..203
NoneNo IPR availablePANTHERPTHR10476:SF57ESCRT-RELATED PROTEIN CHMP1A-RELATEDcoord: 5..203
IPR005024Snf7 familyPFAMPF03357Snf7coord: 11..176
e-value: 3.6E-12
score: 46.2

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lag0003082Lag0003082gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lag0003082.1.exon1Lag0003082.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Lag0003082.1cds.Lag0003082.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lag0003082.1Lag0003082.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007034 vacuolar transport
biological_process GO:0032509 endosome transport via multivesicular body sorting pathway
biological_process GO:0045324 late endosome to vacuole transport
biological_process GO:0015031 protein transport
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body