IVF0021604.2 (mRNA) Melon (IVF77) v1

Overview


Name	IVF0021604.2
Type	mRNA
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	kunitz trypsin inhibitor 1-like
Location	chr05: 23816198 .. 23816779 (+)
Sequence length	582
RNA-Seq Expression	IVF0021604.2
Synteny	IVF0021604.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTACATAAATCACTAGCCATCTTTGGCTATGCATGCCTCTTCATGGCCATAACTTCCACAGCTCAATCGCCTCCAGTGCTTGACACCAATGGTCAACCTCTGCGACGCGGTGTAGAGTACTATATCTCGCCTGCAATTACTGATGTTGGGGGCAATCTCACCCTAAAAAGTCGTAGTAATGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCCGTTACTTCTACAAATATCGGTCTCCCGGTCACCTTCAGGCCGACTGAGGCAGGCAAAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTGAAGCGTTGTCAACATGTGCAACATCGACACAATGGAGAGTGGATGCAACTGAGTCTGACACCGGAAGAAGGTTCGTGGGGATCGGGGATGAGGACGGTCCTGCCGGGATCTTTGGGATCAGCAGGGACAATGGAGCTTACAATATAGTATGGTGTCCTGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGGTGGCTTTGGATGGTGATGCTTTTCCTTTTGAGTTTATCAAAGCTTGA

mRNA sequence

ATGTTACATAAATCACTAGCCATCTTTGGCTATGCATGCCTCTTCATGGCCATAACTTCCACAGCTCAATCGCCTCCAGTGCTTGACACCAATGGTCAACCTCTGCGACGCGGTGTAGAGTACTATATCTCGCCTGCAATTACTGATGTTGGGGGCAATCTCACCCTAAAAAGTCGTAGTAATGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCCGTTACTTCTACAAATATCGGTCTCCCGGTCACCTTCAGGCCGACTGAGGCAGGCAAAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTGAAGCGTTGTCAACATGTGCAACATCGACACAATGGAGAGTGGATGCAACTGAGTCTGACACCGGAAGAAGGTTCGTGGGGATCGGGGATGAGGACGGTCCTGCCGGGATCTTTGGGATCAGCAGGGACAATGGAGCTTACAATATAGTATGGTGTCCTGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGGTGGCTTTGGATGGTGATGCTTTTCCTTTTGAGTTTATCAAAGCTTGA

Coding sequence (CDS)

ATGTTACATAAATCACTAGCCATCTTTGGCTATGCATGCCTCTTCATGGCCATAACTTCCACAGCTCAATCGCCTCCAGTGCTTGACACCAATGGTCAACCTCTGCGACGCGGTGTAGAGTACTATATCTCGCCTGCAATTACTGATGTTGGGGGCAATCTCACCCTAAAAAGTCGTAGTAATGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCCGTTACTTCTACAAATATCGGTCTCCCGGTCACCTTCAGGCCGACTGAGGCAGGCAAAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTGAAGCGTTGTCAACATGTGCAACATCGACACAATGGAGAGTGGATGCAACTGAGTCTGACACCGGAAGAAGGTTCGTGGGGATCGGGGATGAGGACGGTCCTGCCGGGATCTTTGGGATCAGCAGGGACAATGGAGCTTACAATATAGTATGGTGTCCTGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGGTGGCTTTGGATGGTGATGCTTTTCCTTTTGAGTTTATCAAAGCTTGA

Protein sequence

MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA

Homology

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BLAST of IVF0021604.2 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.4e-27
Identity = 68/197 (34.52%), Postives = 110/197 (55.84%), Query Frame = 0


Query: 5   SLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSNAPC 64
           S  IF  A +++ + +T+ +  V+DT+G+P+    +Y+I PAIT  GG+LTL +R++  C
Sbjct: 5   SFTIFILANVWLLVVTTSIAQFVIDTSGEPVENDEDYFIRPAITGNGGSLTLVTRNS--C 64

Query: 65  PLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDATESD 124
           P  VG +P       + ++   +   +D +  G  L ++F+A ++C  ST+WR+   ++ 
Sbjct: 65  PFNVGLDPDAPQGFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDAT 124

Query: 125 TGRRFVGIGDEDGPAGIFG------ISRDNGAYNIVWCPAMM---GRPRCGRAGILVENG 184
           TGRRF+  G +D   G +G       +   G +NI WCP  +    +  CG  GI+ ENG
Sbjct: 125 TGRRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENG 184

Query: 185 VRLVALDGDAFPFEFIK 193
             L+ALDG A P  F K
Sbjct: 185 KILLALDGSALPVAFQK 199

[Close]

BLAST of IVF0021604.2 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 9.1e-27
Identity = 78/204 (38.24%), Postives = 108/204 (52.94%), Query Frame = 0


Query: 1   MLHKSLAIF--GYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKS 60
           M  +SL IF   +  L MA TS AQ   V+DT+G+P+    EY+I PAIT  GG  TL +
Sbjct: 1   MSTRSLTIFILAHVWLLMATTSIAQF--VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRP--TEAGKDIIDEGTSLNIVFEALSTCATSTQW 120
             N PCPL VG +    T +G+ V F P   +   D +     L + F   ++C  ST W
Sbjct: 61  -GNGPCPLHVGLDNTEGT-LGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDW 120

Query: 121 RVDATESDTGRRFVGIGDEDG---PAGIFGI--SRDNGAYNIVWCP---AMMGRPRCGRA 180
           R+   ++ +GRR +  G ++G       F I  ++  G YNI WCP       + +CG  
Sbjct: 121 RLGEKDATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTV 180

Query: 181 GILVENGVRLVALDGDAFPFEFIK 193
           G++ ENG  L+ALDGDA P  F K
Sbjct: 181 GVIRENGKNLLALDGDALPVVFQK 200

[Close]

BLAST of IVF0021604.2 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-26
Identity = 77/200 (38.50%), Postives = 103/200 (51.50%), Query Frame = 0


Query: 4   KSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSNAP 63
           ++L I  + CLF+  T+ AQ   VLDT G+P+    EY+I P IT+ GG  T+ SR N  
Sbjct: 8   RTLIILAHVCLFITTTTIAQF--VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSR-NES 67

Query: 64  CPLFVGQEPVTSTNIGLPVTFRPTEAGKDIID--EGTSLNIVFEALSTCATSTQWRVDAT 123
           CPL VG E +T    GL V F P     D  D      L I F+A S+C  ST+WR+   
Sbjct: 68  CPLHVGLE-LTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEWRLGEK 127

Query: 124 ESDTGRRFVGIGDEDGPAGIFG------ISRDNGAYNIVWCPAMM---GRPRCGRAGILV 183
           ++ +GRR +  G +    G +G       +   G YNI WCP  +    +  CG   +L 
Sbjct: 128 DTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDMLN 187

Query: 184 ENGVRLVALDGDAFPFEFIK 193
           ENG  L+ALDG   P  F K
Sbjct: 188 ENGKILLALDGGPLPLVFQK 203

[Close]

BLAST of IVF0021604.2 vs. ExPASy Swiss-Prot
Match: P16347 (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.6e-13
Identity = 54/143 (37.76%), Postives = 69/143 (48.25%), Query Frame = 0


Query: 25  PPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTF 84
           PPV DT+G  LR    YY+ PA    GG LT+       CPLFV QE       GLPV  
Sbjct: 3   PPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQE-ADGQRDGLPVRI 62

Query: 85  RPTEAGKD--IIDEGTSLNIVFEALSTCATSTQWRVDATESDTGRRFVGIGD--EDGPAG 144
            P        II   T + I F A +TC  ST+W +D +E  +GRR V  G   +  P+G
Sbjct: 63  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHID-SELVSGRRHVITGPVRDPSPSG 122

Query: 145 ---IFGISRDNGA----YNIVWC 157
               F I + +GA    Y ++ C
Sbjct: 123 RENAFRIEKYSGAEVHEYKLMAC 143

[Close]

BLAST of IVF0021604.2 vs. ExPASy Swiss-Prot
Match: P07596 (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 75.9 bits (185), Expect = 5.7e-13
Identity = 52/146 (35.62%), Postives = 67/146 (45.89%), Query Frame = 0


Query: 22  AQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLP 81
           A  PPV DT+G  LR    YY+  A    GG LT+       CPLFV Q+P    + G P
Sbjct: 23  ADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP-NGQHDGFP 82

Query: 82  VTFRP--TEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDATESDTGRRFVGIGDEDGPA 141
           V   P        II   T + I F A +TC  ST+W +D +E   GRR V  G    P+
Sbjct: 83  VRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHID-SELAAGRRHVITGPVKDPS 142

Query: 142 -----GIFGISRDNGA----YNIVWC 157
                  F I + +GA    Y ++ C
Sbjct: 143 PSGRENAFRIEKYSGAEVHEYKLMSC 166

[Close]

BLAST of IVF0021604.2 vs. ExPASy TrEMBL
Match: A0A5D3D468 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520 PE=3 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.4e-107
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60
           MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS
Sbjct: 3   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 62

Query: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120
           NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA
Sbjct: 63  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 122

Query: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180
           TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA
Sbjct: 123 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 182

Query: 181 LDGDAFPFEFIKA 194
           LDGDAFPFEFIKA
Sbjct: 183 LDGDAFPFEFIKA 195

[Close]

BLAST of IVF0021604.2 vs. ExPASy TrEMBL
Match: A0A5A7VDV8 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530 PE=3 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.4e-107
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60
           MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS
Sbjct: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60

Query: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120
           NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA
Sbjct: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120

Query: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180
           TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA
Sbjct: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180

Query: 181 LDGDAFPFEFIKA 194
           LDGDAFPFEFIKA
Sbjct: 181 LDGDAFPFEFIKA 193

[Close]

BLAST of IVF0021604.2 vs. ExPASy TrEMBL
Match: A0A0A0LIK2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 4.7e-95
Identity = 172/178 (96.63%), Postives = 174/178 (97.75%), Query Frame = 0


Query: 16  MAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 75
           MAITSTAQ PPVLDTNGQPL+RGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 76  TNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDATESDTGRRFVGIGDE 135
           TNIGLPVTFRPTE G DIIDEGTSLNIVF+ALSTC TSTQWRVDATESDTGRRFVGIGDE
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 136 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 194
           DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

[Close]

BLAST of IVF0021604.2 vs. ExPASy TrEMBL
Match: A0A6J1G3G3 (miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 5.4e-83
Identity = 148/193 (76.68%), Postives = 167/193 (86.53%), Query Frame = 0


Query: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60
           MLH SLAIF +  +FMAITSTAQ PPVLDT+GQPLRRGVEYYI PAITDV GNLTLKSRS
Sbjct: 1   MLHNSLAIFSFLLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120
           NAPCPL+VGQEP+ STNIGLPVTF P  AG+DII+E    N+VF+A STC TSTQWRVD 
Sbjct: 61  NAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDE 120

Query: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180
            ES TGRRFVG+G+++GP GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+A
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 181 LDGDAFPFEFIKA 194
           LDG+AFPFEF+KA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of IVF0021604.2 vs. ExPASy TrEMBL
Match: A0A6J1KC85 (miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 7.8e-82
Identity = 146/193 (75.65%), Postives = 166/193 (86.01%), Query Frame = 0


Query: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60
           MLH SLAIF Y  +F+AITSTAQ PPVLDT+GQPLRRGVEYYI PAIT+V GNLTLK+RS
Sbjct: 1   MLHNSLAIFSYLLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRS 60

Query: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120
           NAPCPL+VGQEPV STNIGLPVTF P  AG+DII+E    N+ F+A STC TSTQWRVD 
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180
            ES TGRRFVG+G+++GP GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+A
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 181 LDGDAFPFEFIKA 194
           LDG+AFPFEF+KA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of IVF0021604.2 vs. NCBI nr
Match: KAA0063689.1 (miraculin-like [Cucumis melo var. makuwa] >TYK18338.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 397 bits (1019), Expect = 1.78e-139
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60
           MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS
Sbjct: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60

Query: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120
           NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA
Sbjct: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120

Query: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180
           TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA
Sbjct: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180

Query: 181 LDGDAFPFEFIKA 193
           LDGDAFPFEFIKA
Sbjct: 181 LDGDAFPFEFIKA 193

[Close]

BLAST of IVF0021604.2 vs. NCBI nr
Match: KAA0063690.1 (miraculin-like [Cucumis melo var. makuwa] >TYK18337.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 397 bits (1019), Expect = 1.91e-139
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60
           MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS
Sbjct: 3   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 62

Query: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120
           NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA
Sbjct: 63  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 122

Query: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180
           TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA
Sbjct: 123 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 182

Query: 181 LDGDAFPFEFIKA 193
           LDGDAFPFEFIKA
Sbjct: 183 LDGDAFPFEFIKA 195

[Close]

BLAST of IVF0021604.2 vs. NCBI nr
Match: XP_004139192.1 (miraculin [Cucumis sativus])

HSP 1 Score: 385 bits (989), Expect = 7.19e-135
Identity = 187/193 (96.89%), Postives = 189/193 (97.93%), Query Frame = 0


Query: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60
           MLHKSLAIFGYACLFMAITSTAQ PPVLDTNGQPL+RGVEYYISPAITDVGGNLTLKSRS
Sbjct: 3   MLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRS 62

Query: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120
           NAPCPLFVGQEPVTSTNIGLPVTFRPTE G DIIDEGTSLNIVF+ALSTC TSTQWRVDA
Sbjct: 63  NAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDA 122

Query: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180
           TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA
Sbjct: 123 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 182

Query: 181 LDGDAFPFEFIKA 193
           LDGDAFPFEFIKA
Sbjct: 183 LDGDAFPFEFIKA 195

[Close]

BLAST of IVF0021604.2 vs. NCBI nr
Match: KGN60910.2 (hypothetical protein Csa_023447 [Cucumis sativus])

HSP 1 Score: 385 bits (989), Expect = 2.70e-132
Identity = 187/193 (96.89%), Postives = 189/193 (97.93%), Query Frame = 0


Query: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60
           MLHKSLAIFGYACLFMAITSTAQ PPVLDTNGQPL+RGVEYYISPAITDVGGNLTLKSRS
Sbjct: 172 MLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRS 231

Query: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120
           NAPCPLFVGQEPVTSTNIGLPVTFRPTE G DIIDEGTSLNIVF+ALSTC TSTQWRVDA
Sbjct: 232 NAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDA 291

Query: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180
           TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA
Sbjct: 292 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 351

Query: 181 LDGDAFPFEFIKA 193
           LDGDAFPFEFIKA
Sbjct: 352 LDGDAFPFEFIKA 364

[Close]

BLAST of IVF0021604.2 vs. NCBI nr
Match: XP_038888526.1 (kunitz type trypsin inhibitor 106-like [Benincasa hispida])

HSP 1 Score: 352 bits (903), Expect = 9.31e-122
Identity = 169/193 (87.56%), Postives = 178/193 (92.23%), Query Frame = 0


Query: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60
           MLHKSLAIFGYACLFMAITSTAQ PPVLDT+ QPLRRGVEYYI PAITDVGGNLTLKSRS
Sbjct: 3   MLHKSLAIFGYACLFMAITSTAQLPPVLDTDSQPLRRGVEYYIKPAITDVGGNLTLKSRS 62

Query: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120
           NAPCPLFVGQEPVTSTNIGLPVTF P  AG+DII+E  SLNIVFEALSTC TSTQWRVD 
Sbjct: 63  NAPCPLFVGQEPVTSTNIGLPVTFTPIVAGEDIIEESRSLNIVFEALSTCVTSTQWRVDE 122

Query: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180
            ESDTGRRFVGIG++DGP+GIF I R+NG YNIVWCPAMMGRPRCG AGILVENGVRL+A
Sbjct: 123 AESDTGRRFVGIGNDDGPSGIFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVENGVRLLA 182

Query: 181 LDGDAFPFEFIKA 193
           LDGDAFPFEF+KA
Sbjct: 183 LDGDAFPFEFVKA 195

[Close]

BLAST of IVF0021604.2 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 77.0 bits (188), Expect = 1.8e-14
Identity = 59/191 (30.89%), Postives = 94/191 (49.21%), Query Frame = 0


Query: 12  ACLFMAITSTAQSPP--VLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVG 71
           + +F A ++   +P   VLD  G P++  V+YYI PA    GG L   +R+ +   L + 
Sbjct: 15  SAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLCLN 74

Query: 72  QEPVTSTN---IGLPVTFRPTEAGKDIIDEGTSLNIVFEA-LSTCATSTQWRVDATESDT 131
            + V S++    GLPVTF P       +    SLN+ F++ +  C  S  WR+D +    
Sbjct: 75  LDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDHS-VQL 134

Query: 132 GRRFVGIGDEDGPAGI-FGISRDNGAYNIVWCPAMMGRPRCGRAGILV-ENGVRLVALDG 191
            + FV IG + G     F I  D  AY +++CP +     C    + + ++GVR + L  
Sbjct: 135 RKSFVSIGGQKGKGNSWFQIQEDGDAYKLMYCP-ISSIVACINVSLEIDDHGVRRLVLST 194

Query: 192 D-AFPFEFIKA 194
           D +F  +F KA
Sbjct: 195 DQSFVVKFQKA 203

[Close]

BLAST of IVF0021604.2 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 72.0 bits (175), Expect = 5.8e-13
Identity = 57/187 (30.48%), Postives = 83/187 (44.39%), Query Frame = 0


Query: 6   LAIFGYACLFMA---ITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSNA 65
           L IF    +F++   +T+ A   PV D NG+ L  GV YYI P I   GG LT+ +    
Sbjct: 5   LYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTE 64

Query: 66  PCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDATE 125
            CP  V Q+     + GLPV F P +  +  I   T +NI F   S       W +   +
Sbjct: 65  TCPTSVIQDQF-EVSQGLPVKFSPYDKSR-TIPVSTDVNIKFSPTSI------WELANFD 124

Query: 126 SDTGRRFVGIGDEDGPAG------IFGISRDNGAYNIVWCPAMMG--RPRCGRAGILVEN 182
             T + F+     +G  G       F I +    Y I +CP +    +  C   G+ V++
Sbjct: 125 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

The following BLAST results are available for this feature:

BLAST of IVF0021604.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV7	2.4e-27	34.52	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
A0A072TH68	9.1e-27	38.24	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
G7LCV1	1.5e-26	38.50	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
P16347	2.6e-13	37.76	Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 ...	[more]
P07596	5.7e-13	35.62	Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2	[more]

BLAST of IVF0021604.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D468	1.4e-107	100.00	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520...	[more]
A0A5A7VDV8	1.4e-107	100.00	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530...	[more]
A0A0A0LIK2	4.7e-95	96.63	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=3 SV=1	[more]
A0A6J1G3G3	5.4e-83	76.68	miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1	[more]
A0A6J1KC85	7.8e-82	75.65	miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1	[more]

BLAST of IVF0021604.2 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0063689.1	1.78e-139	100.00	miraculin-like [Cucumis melo var. makuwa] >TYK18338.1 miraculin-like [Cucumis me...	[more]
KAA0063690.1	1.91e-139	100.00	miraculin-like [Cucumis melo var. makuwa] >TYK18337.1 miraculin-like [Cucumis me...	[more]
XP_004139192.1	7.19e-135	96.89	miraculin [Cucumis sativus]	[more]
KGN60910.2	2.70e-132	96.89	hypothetical protein Csa_023447 [Cucumis sativus]	[more]
XP_038888526.1	9.31e-122	87.56	kunitz type trypsin inhibitor 106-like [Benincasa hispida]	[more]

BLAST of IVF0021604.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73325.1	1.8e-14	30.89	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G17860.1	5.8e-13	30.48	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 26..193 e-value: 1.1E-30 score: 118.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 27..192 e-value: 3.5E-40 score: 137.8
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..192
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 27..43
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 26..192 e-value: 7.07161E-40 score: 132.081
None	No IPR available	GENE3D	2.80.10.50		coord: 21..193 e-value: 6.9E-47 score: 161.3
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 8..192
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 25..193

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
IVF0021604	IVF0021604	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
IVF0021604.2-exon	IVF0021604.2-exon-chr05:23816198..23816779	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
IVF0021604.2-cds	IVF0021604.2-cds-chr05:23816198..23816779	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
IVF0021604.2	IVF0021604.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0007165	signal transduction
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0003953	NAD+ nucleosidase activity