HG10005644.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10005644.1 vs. NCBI nr
Match: XP_023529731.1 (uncharacterized protein LOC111792465 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 576.6 bits (1485), Expect = 1.6e-160
Identity = 310/383 (80.94%), Postives = 327/383 (85.38%), Query Frame = 0

Query: 52  MERLLSSPSPTSLKINPNPSLILPRFRRIDSVKLNFPLPTRHGLKPLGLISCKNEKPSIS 111
           M+RLL SPSPTS KINPNP L+LPRFRRIDS KLNFPLPTRHGL+PLGL S K EKPS+ 
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKTEKPSMP 60

Query: 112 LIRCFSSSNSSTDSICQSDSRAGSPNPDFLKHFAGKEKVKRSLFVLSALALILIQPVFAP 171
           LI C SSSNSS+DSI  SDSRAGSP PD L+HFAGK+KVK+SLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSSSDSISTSDSRAGSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 172 AAFASFQNAAATTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 231
           AAFASFQN AA TGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 AAFASFQN-AAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 232 GCTRMESAAVGALWGCGHDA------------------------------------GIMG 291
           GC+RMESAAVGALWGCGHDA                                    G++G
Sbjct: 181 GCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLG 240

Query: 292 IREASEVRTPCVVGLDNGECDVGIYETLEKPMVVG----KKKKLGFATFATGVVHGLQPD 351
           IREASEVRTPCVVGL+NGECDVGIY+TLEKPMV+G    KKKK+GFATFATGVVHGLQPD
Sbjct: 241 IREASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQPD 300

Query: 352 ALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWA 395
           ALMIVLPALALPSRVAGAAFL+MFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTWA
Sbjct: 301 ALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWA 360

BLAST of HG10005644.1 vs. NCBI nr
Match: XP_022989236.1 (uncharacterized protein LOC111486376 [Cucurbita maxima])

HSP 1 Score: 575.5 bits (1482), Expect = 3.6e-160
Identity = 310/383 (80.94%), Postives = 328/383 (85.64%), Query Frame = 0

Query: 52  MERLLSSPSPTSLKINPNPSLILPRFRRIDSVKLNFPLPTRHGLKPLGLISCKNEKPSIS 111
           M+RLL SPSPTS KINPNP L+LPRFRRIDSVKLNFPLPTRHGL+PLGL+S K EKPS+ 
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSVKLNFPLPTRHGLRPLGLLSSKVEKPSMP 60

Query: 112 LIRCFSSSNSSTDSICQSDSRAGSPNPDFLKHFAGKEKVKRSLFVLSALALILIQPVFAP 171
           LI C SSSNSS+DSI  SDSRA SP PD L+HFAGK+KVK+SLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSSSDSISTSDSRASSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 172 AAFASFQNAAATTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 231
           AAFASFQN AA TGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 AAFASFQN-AAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 232 GCTRMESAAVGALWGCGHDA------------------------------------GIMG 291
           GC+RMESAAVGALWGCGHDA                                    G++G
Sbjct: 181 GCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLG 240

Query: 292 IREASEVRTPCVVGLDNGECDVGIYETLEKPMVVG----KKKKLGFATFATGVVHGLQPD 351
           IREASEVRTPCVVGL+NGECDVGIY+TLEKPMV+G    KKKK+GFATFATGVVHGLQPD
Sbjct: 241 IREASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQPD 300

Query: 352 ALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWA 395
           ALMIVLPALALPSRVAGAAFL+MFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTWA
Sbjct: 301 ALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWA 360

BLAST of HG10005644.1 vs. NCBI nr
Match: KAG6572115.1 (hypothetical protein SDJN03_28843, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 575.5 bits (1482), Expect = 3.6e-160
Identity = 307/345 (88.99%), Postives = 316/345 (91.59%), Query Frame = 0

Query: 52  MERLLSSPSPTSLKINPNPSLILPRFRRIDSVKLNFPLPTRHGLKPLGLISCKNEKPSIS 111
           MERLL SPSPTSLKIN N SL LP F RIDS+KLNFPLPTRHGLKPLGL  CKNEKPS S
Sbjct: 1   MERLLYSPSPTSLKINLNSSLTLPWFCRIDSLKLNFPLPTRHGLKPLGLTFCKNEKPSRS 60

Query: 112 LIRCFSSSNSSTDSICQSDSRAGSPNPDFLKHFAGKEKVKRSLFVLSALALILIQPVFAP 171
              C SSSNSS+D I  SDS AGSP PDFL+HFAGKEKVKRSL VLSAL LILIQPVFAP
Sbjct: 61  SFCCLSSSNSSSDLIFPSDSLAGSPKPDFLEHFAGKEKVKRSLCVLSALVLILIQPVFAP 120

Query: 172 AAFASFQNAAATTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 231
            AFASFQNAAA TGGPAAA VGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 EAFASFQNAAAKTGGPAAAVVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 232 GCTRMESAAVGALWGCGHDAGIMGIREASEVRTPCVVGLDNGECDVGIYETLEKPMVVGK 291
           GC RMESAAVGALWGCGHDAG+MGIRE SEVRTPC V L+NGECDVGIYETLEKPMVVGK
Sbjct: 181 GCNRMESAAVGALWGCGHDAGVMGIREVSEVRTPC-VALENGECDVGIYETLEKPMVVGK 240

Query: 292 --KKKLGFATFATGVVHGLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAF 351
             KKK+GFATFATGVVHGLQPDALMIVLPALALPSRVAGAAFLLMFLVGTV+SMGSYTAF
Sbjct: 241 KRKKKIGFATFATGVVHGLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVLSMGSYTAF 300

Query: 352 IGSFSEALKDRVPRITERLTWAASFVAIALGLAIIISQFLGYSLY 395
           IGSFSEALKDRVPRITERLTWAASFVAIALGLAIII+QFLG+SLY
Sbjct: 301 IGSFSEALKDRVPRITERLTWAASFVAIALGLAIIITQFLGFSLY 344

BLAST of HG10005644.1 vs. NCBI nr
Match: KAG6589014.1 (hypothetical protein SDJN03_17579, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015277.1 ureH [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 568.9 bits (1465), Expect = 3.3e-158
Identity = 307/383 (80.16%), Postives = 325/383 (84.86%), Query Frame = 0

Query: 52  MERLLSSPSPTSLKINPNPSLILPRFRRIDSVKLNFPLPTRHGLKPLGLISCKNEKPSIS 111
           M+RLL SPSPTS KINPNP L+LPRFRRIDS KLNFPLPTRHGL+PLGL S K +KPS+ 
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60

Query: 112 LIRCFSSSNSSTDSICQSDSRAGSPNPDFLKHFAGKEKVKRSLFVLSALALILIQPVFAP 171
           LI C SSSNSS+DSI  SDSRA SP PD L+HFAGK+KVK+SLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 172 AAFASFQNAAATTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 231
           AAFASFQN AA TGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 AAFASFQN-AAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 232 GCTRMESAAVGALWGCGHDA------------------------------------GIMG 291
           GC+RMESAAVGALWGCGHDA                                    G++G
Sbjct: 181 GCSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLG 240

Query: 292 IREASEVRTPCVVGLDNGECDVGIYETLEKPMVVG----KKKKLGFATFATGVVHGLQPD 351
           IREASEVRTPCVVGL+NGECDVGIY+TLE PMV+G    KKKK+GFATFATGVVHGLQPD
Sbjct: 241 IREASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPD 300

Query: 352 ALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWA 395
           ALMIVLPALALPSRVAGAAFL+MFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTWA
Sbjct: 301 ALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWA 360

BLAST of HG10005644.1 vs. NCBI nr
Match: KAA0049756.1 (NicO domain-containing protein [Cucumis melo var. makuwa] >TYK12122.1 NicO domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 565.8 bits (1457), Expect = 2.8e-157
Identity = 319/388 (82.22%), Postives = 332/388 (85.57%), Query Frame = 0

Query: 11  LTWTLSSKFQLHQPASFRGHLRPFFFLRRRRRRLSRRRTDSMER-LLSSPSPTSLKINPN 70
           L+ +LSSKFQ   P +    + P       RRR SRR T SMER LL S SPTSL INPN
Sbjct: 18  LSLSLSSKFQ--TPPTQGSLISP----PPPRRRPSRRPTSSMERLLLYSSSPTSLNINPN 77

Query: 71  PSLILPRFRR-IDSVKLNFPLPTRHGLKPLGLISCKNEKPSISLIRCFSSSNSSTDSICQ 130
            SL+LPRF R I S++ NFPLPTRHG  PLG +S KN+KPS S I CFSS NSS DSI Q
Sbjct: 78  SSLVLPRFARIIHSLRPNFPLPTRHGFHPLGFLSSKNDKPSFSSIPCFSSPNSSADSIFQ 137

Query: 131 SDSRAGSPNPDFLKHFAGKEKVKRSLFVLSALALILIQPVFAPAAFASFQNAAATTGGPA 190
           SDS   SP PDFLK FA KEKVK S FVLSALALILIQPVFAPAAFASFQN AA TGGPA
Sbjct: 138 SDSPPDSPKPDFLKPFARKEKVKSSFFVLSALALILIQPVFAPAAFASFQN-AAKTGGPA 197

Query: 191 AAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIGCTRMESAAVGALWGCG 250
           A AVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSIGC+RMESAAVGALWGCG
Sbjct: 198 AVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIGCSRMESAAVGALWGCG 257

Query: 251 HDAGIMGIREASEVRTPCVVGLDNGECDVGIYETLEKPMVVG--KKKKLGFATFATGVVH 310
           HDAGIMGIREASE+RTP VV LDNGECDVGIYETLEKPMVVG  K KKLGFATFATGVVH
Sbjct: 258 HDAGIMGIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVH 317

Query: 311 GLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE 370
           GLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIG+FSEALKDRVPRITE
Sbjct: 318 GLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGTFSEALKDRVPRITE 377

Query: 371 RLTWAASFVAIALGLAIIISQFLGYSLY 395
           RLTW ASFVAIALGLAIIISQFLGYSLY
Sbjct: 378 RLTWVASFVAIALGLAIIISQFLGYSLY 398

BLAST of HG10005644.1 vs. ExPASy Swiss-Prot
Match: Q07404 (Urease accessory protein UreH OS=Bacillus sp. (strain TB-90) OX=36824 GN=ureH PE=3 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 9.9e-04
Identity = 50/211 (23.70%), Postives = 83/211 (39.34%), Query Frame = 0

Query: 198 QSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIGCTRM-ESAAVGALWGCGHDAGIM-- 257
           Q +LLS    GF  G  H +  PDH+ A++ +     ++  S+  G  WG GH + ++  
Sbjct: 3   QVDLLSILTLGFVLGIKHAME-PDHVIAVSTIVCQSKKLWRSSLAGVFWGIGHTSTLLIF 62

Query: 258 ---------GIREASEVRTPCVVGLDNGECDVGIYETLEK---------------PMVVG 317
                     I +   +    +VG+      +    +L+K               P+   
Sbjct: 63  GMTIILMKKKISQEWSMSLEFLVGIILVYFGISAILSLKKTHEHSHSRLHLHTDHPIYTY 122

Query: 318 KKKKLGFATFATGVVHGLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFI 377
           K      + F  G++HGL   A M++L    +     G  ++L F  GTV+ M S+T  I
Sbjct: 123 KGIPYVKSLF-IGIIHGLAGSAAMVLLTMSTVEKAWEGLLYILFFGAGTVLGMLSFTTLI 182

Query: 378 GSFSEALKDRVPRITERLTWAASFVAIALGL 382
           G     L  R  RI         F++   G+
Sbjct: 183 G-IPFTLSARKIRIHNAFIQITGFISTVFGI 210

BLAST of HG10005644.1 vs. ExPASy TrEMBL
Match: A0A6J1JJH7 (uncharacterized protein LOC111486376 OS=Cucurbita maxima OX=3661 GN=LOC111486376 PE=4 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.7e-160
Identity = 310/383 (80.94%), Postives = 328/383 (85.64%), Query Frame = 0

Query: 52  MERLLSSPSPTSLKINPNPSLILPRFRRIDSVKLNFPLPTRHGLKPLGLISCKNEKPSIS 111
           M+RLL SPSPTS KINPNP L+LPRFRRIDSVKLNFPLPTRHGL+PLGL+S K EKPS+ 
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSVKLNFPLPTRHGLRPLGLLSSKVEKPSMP 60

Query: 112 LIRCFSSSNSSTDSICQSDSRAGSPNPDFLKHFAGKEKVKRSLFVLSALALILIQPVFAP 171
           LI C SSSNSS+DSI  SDSRA SP PD L+HFAGK+KVK+SLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSSSDSISTSDSRASSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 172 AAFASFQNAAATTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 231
           AAFASFQN AA TGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 AAFASFQN-AAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 232 GCTRMESAAVGALWGCGHDA------------------------------------GIMG 291
           GC+RMESAAVGALWGCGHDA                                    G++G
Sbjct: 181 GCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLG 240

Query: 292 IREASEVRTPCVVGLDNGECDVGIYETLEKPMVVG----KKKKLGFATFATGVVHGLQPD 351
           IREASEVRTPCVVGL+NGECDVGIY+TLEKPMV+G    KKKK+GFATFATGVVHGLQPD
Sbjct: 241 IREASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQPD 300

Query: 352 ALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWA 395
           ALMIVLPALALPSRVAGAAFL+MFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTWA
Sbjct: 301 ALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWA 360

BLAST of HG10005644.1 vs. ExPASy TrEMBL
Match: A0A5A7U6H4 (NicO domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00560 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.4e-157
Identity = 319/388 (82.22%), Postives = 332/388 (85.57%), Query Frame = 0

Query: 11  LTWTLSSKFQLHQPASFRGHLRPFFFLRRRRRRLSRRRTDSMER-LLSSPSPTSLKINPN 70
           L+ +LSSKFQ   P +    + P       RRR SRR T SMER LL S SPTSL INPN
Sbjct: 18  LSLSLSSKFQ--TPPTQGSLISP----PPPRRRPSRRPTSSMERLLLYSSSPTSLNINPN 77

Query: 71  PSLILPRFRR-IDSVKLNFPLPTRHGLKPLGLISCKNEKPSISLIRCFSSSNSSTDSICQ 130
            SL+LPRF R I S++ NFPLPTRHG  PLG +S KN+KPS S I CFSS NSS DSI Q
Sbjct: 78  SSLVLPRFARIIHSLRPNFPLPTRHGFHPLGFLSSKNDKPSFSSIPCFSSPNSSADSIFQ 137

Query: 131 SDSRAGSPNPDFLKHFAGKEKVKRSLFVLSALALILIQPVFAPAAFASFQNAAATTGGPA 190
           SDS   SP PDFLK FA KEKVK S FVLSALALILIQPVFAPAAFASFQN AA TGGPA
Sbjct: 138 SDSPPDSPKPDFLKPFARKEKVKSSFFVLSALALILIQPVFAPAAFASFQN-AAKTGGPA 197

Query: 191 AAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIGCTRMESAAVGALWGCG 250
           A AVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSIGC+RMESAAVGALWGCG
Sbjct: 198 AVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIGCSRMESAAVGALWGCG 257

Query: 251 HDAGIMGIREASEVRTPCVVGLDNGECDVGIYETLEKPMVVG--KKKKLGFATFATGVVH 310
           HDAGIMGIREASE+RTP VV LDNGECDVGIYETLEKPMVVG  K KKLGFATFATGVVH
Sbjct: 258 HDAGIMGIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVH 317

Query: 311 GLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE 370
           GLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIG+FSEALKDRVPRITE
Sbjct: 318 GLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGTFSEALKDRVPRITE 377

Query: 371 RLTWAASFVAIALGLAIIISQFLGYSLY 395
           RLTW ASFVAIALGLAIIISQFLGYSLY
Sbjct: 378 RLTWVASFVAIALGLAIIISQFLGYSLY 398

BLAST of HG10005644.1 vs. ExPASy TrEMBL
Match: A0A6J1EK24 (uncharacterized protein LOC111435202 OS=Cucurbita moschata OX=3662 GN=LOC111435202 PE=4 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.3e-157
Identity = 306/383 (79.90%), Postives = 324/383 (84.60%), Query Frame = 0

Query: 52  MERLLSSPSPTSLKINPNPSLILPRFRRIDSVKLNFPLPTRHGLKPLGLISCKNEKPSIS 111
           M+RLL SPSPTS KINPNP L+LPRFRRIDS KLNFPLPTRHGL+PLGL S K +KPS+ 
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60

Query: 112 LIRCFSSSNSSTDSICQSDSRAGSPNPDFLKHFAGKEKVKRSLFVLSALALILIQPVFAP 171
           LI C SSSNS +DSI  S+SRA SP PD L+HFAGK+KVK+SLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSLSDSISTSNSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 172 AAFASFQNAAATTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 231
           AAFASFQN AA TGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 AAFASFQN-AAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 232 GCTRMESAAVGALWGCGHDA------------------------------------GIMG 291
           GC+RMESAAVGALWGCGHDA                                    GI+G
Sbjct: 181 GCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGILG 240

Query: 292 IREASEVRTPCVVGLDNGECDVGIYETLEKPMVVG----KKKKLGFATFATGVVHGLQPD 351
           IREASEVRTPCVVGL+NGECDVGIY+TLE PMV+G    KKKK+GFATFATGVVHGLQPD
Sbjct: 241 IREASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPD 300

Query: 352 ALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWA 395
           ALMIVLPALALPSRVAGAAFL+MFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTWA
Sbjct: 301 ALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWA 360

BLAST of HG10005644.1 vs. ExPASy TrEMBL
Match: A0A6J1C3Z9 (uncharacterized protein LOC111007714 OS=Momordica charantia OX=3673 GN=LOC111007714 PE=4 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 5.0e-152
Identity = 301/379 (79.42%), Postives = 315/379 (83.11%), Query Frame = 0

Query: 52  MERLLSSPSPTSLKINPNPSLILPRFRRIDSVKLNFPLPTRHGLKPLGLISCKNEKPSIS 111
           MERLL SPSPTSLKINP+ SL LPRFR I S KLNFP+PTRHGL+ +GLISCKNEKP +S
Sbjct: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60

Query: 112 LIRCFSSSNSSTDSICQSDSRAGSPNPDFLKHFAGKEKVKRSLFVLSALALILIQPVFAP 171
            IR  S  NSS+DSIC SDSRA SP  D L+HF GKEKVKRSLFVLSALALILIQPVFAP
Sbjct: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120

Query: 172 AAFASFQNAAATTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 231
           AA ASF N AA TG PAAAAVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 AASASFPN-AAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 232 GCTRMESAAVGALWGCGHDA------------------------------------GIMG 291
           GC RMESAAVGALWGCGHDA                                    G+MG
Sbjct: 181 GCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMG 240

Query: 292 IREASEVRTPCVVGLDNGECDVGIYETLEKPMVVGKKKKLGFATFATGVVHGLQPDALMI 351
           IREASEVRTPC V L+NGECDVGI+ETL+KPM VGKKKKLGFATFATGVVHGLQPDALMI
Sbjct: 241 IREASEVRTPC-VALENGECDVGIFETLDKPM-VGKKKKLGFATFATGVVHGLQPDALMI 300

Query: 352 VLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAASFV 395
           VLPALALPSR+AGAAFL+MFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAASFV
Sbjct: 301 VLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAASFV 360

BLAST of HG10005644.1 vs. ExPASy TrEMBL
Match: A0A6J1I158 (uncharacterized protein LOC111468477 OS=Cucurbita maxima OX=3661 GN=LOC111468477 PE=4 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 2.1e-150
Identity = 301/381 (79.00%), Postives = 311/381 (81.63%), Query Frame = 0

Query: 52  MERLLSSPSPTSLKINPNPSLILPRFRRIDSVKLNFPLPTRHGLKPLGLISCKNEKPSIS 111
           MERLL SPSPTSLKIN N SL L    RIDS+KLNFPLPT HGLKPLGL  CKNEKPS+S
Sbjct: 1   MERLLYSPSPTSLKINLNSSLTLLWSGRIDSLKLNFPLPTLHGLKPLGLTFCKNEKPSMS 60

Query: 112 LIRCFSSSNSSTDSICQSDSRAGSPNPDFLKHFAGKEKVKRSLFVLSALALILIQPVFAP 171
              C SSSN  +DSI  SDS AGSP PDFL+HFAGKEKVKRSL VLSAL LILIQP  AP
Sbjct: 61  SFCCLSSSNWLSDSIFPSDSLAGSPKPDFLEHFAGKEKVKRSLCVLSALVLILIQPGLAP 120

Query: 172 AAFASFQNAAATTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 231
           AAFASFQNAAA TGGPAAA VGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 AAFASFQNAAAKTGGPAAAVVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 232 GCTRMESAAVGALWGCGHDA------------------------------------GIMG 291
           GC RMESAAVGALWGCGHDA                                    G+MG
Sbjct: 181 GCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGMTLFIIGVMG 240

Query: 292 IREASEVRTPCVVGLDNGECDVGIYETLEKPMVVG--KKKKLGFATFATGVVHGLQPDAL 351
           IRE SEVRTPC V L+NGECDVGIYETLEKPMVVG  KKKK+GFATFATGVVHGLQPDAL
Sbjct: 241 IREVSEVRTPC-VALENGECDVGIYETLEKPMVVGKKKKKKIGFATFATGVVHGLQPDAL 300

Query: 352 MIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAAS 395
           MIVLPALALPSRVAGAAFLLMFLVGTV+SMGSYTAFIGSFSEALKDRVPRITERLTWAAS
Sbjct: 301 MIVLPALALPSRVAGAAFLLMFLVGTVLSMGSYTAFIGSFSEALKDRVPRITERLTWAAS 360

BLAST of HG10005644.1 vs. TAIR 10
Match: AT2G16800.1 (high-affinity nickel-transport family protein )

HSP 1 Score: 331.6 bits (849), Expect = 8.4e-91
Identity = 203/393 (51.65%), Postives = 252/393 (64.12%), Query Frame = 0

Query: 52  MERLLSSPS-----PTSLKINPNPSLILPRFRRIDSVKLN-FPLPTRHGLKPLGLISC-- 111
           M+RLL  PS     P+  +  P+P L+     R+ S  L+ FP   R   + +  ISC  
Sbjct: 1   MDRLLQPPSSHSIAPSKFQSRPSPLLL----HRLHSTNLSTFPSSRRLESRRISSISCFF 60

Query: 112 -KNEKPSISLIRCFSSSNSSTDSICQSDSRAGSPNPDFLKHFAGKEKVKRSL-----FVL 171
            +N  P  S       + SS   I  S + A  PNP F++      + ++++      ++
Sbjct: 61  RQNPSPDTSP----GLNQSSNFLIASSQTDASKPNPGFIQRIVSSFEQRKTISAGMVILV 120

Query: 172 SALALILIQPVFAPAAFASFQNAAATTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTL 231
           SA+A +L+ P+  P AFASFQ A   T    AA VG +L+++E+L+SAWTGFFAGCLHTL
Sbjct: 121 SAIAALLLNPILVPPAFASFQTA---TNSGGAAVVGGKLLRTEVLTSAWTGFFAGCLHTL 180

Query: 232 SGPDHLAALAPLSIGCTRMESAAVGALWGCGHDA-------------------------- 291
           SGPDHLAALAPLSIG TRMESAAVGALWGCGHDA                          
Sbjct: 181 SGPDHLAALAPLSIGRTRMESAAVGALWGCGHDAGQLIFGLLFLLLKDRLHIEVIRTWGT 240

Query: 292 ----------GIMGIREASEVRTPCVVGLDNGECDVGIYETLEKPMVVGKKKKLGFATFA 351
                     G MGI+EASE+  PCVV L+NGE D       EK +   KKKK+GFATFA
Sbjct: 241 RVVGLTLLVIGAMGIKEASEMPEPCVVTLENGETD-------EKSL---KKKKIGFATFA 300

Query: 352 TGVVHGLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRV 395
           TG+VHGLQPDALM+VLPALALPSR+AGA+FL+MFL+GTVI+MGSYT FIGS SEALK++V
Sbjct: 301 TGIVHGLQPDALMMVLPALALPSRIAGASFLIMFLIGTVIAMGSYTVFIGSCSEALKEKV 360

BLAST of HG10005644.1 vs. TAIR 10
Match: AT4G35080.1 (high-affinity nickel-transport family protein )

HSP 1 Score: 307.8 bits (787), Expect = 1.3e-83
Identity = 195/388 (50.26%), Postives = 244/388 (62.89%), Query Frame = 0

Query: 52  MERLLSSPSPTSLKINPN--PSLILPRFRRIDSVKLNFPLPTRHGLKPLGLISCKNEK-P 111
           MERLL  PS +S  I+P+  PS   P   R+ S  L+F    R   + +  ISC + + P
Sbjct: 1   MERLL-QPSSSSSSISPSKFPSRTSPFLPRLRSSGLSFVSTHRPESRRVSSISCNSSQIP 60

Query: 112 SISLIRCFSSSNSSTDSICQSDSRAGSPNPDFLKHFAGKEKVKR------SLFVLSALAL 171
           S+      +++N+S +   +SD     PNP FL   A     +R      ++ ++SA+A+
Sbjct: 61  SLYTPIGSNTTNNSFNGSPKSDE--SKPNPGFLTRIATSASEQRKTLSTGTVILISAVAV 120

Query: 172 ILIQPVFAPAAFASFQNAAATTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDH 231
           +L+ P+ AP AFASFQ AA                +S  L+SAWTGF AGCLHTLSGPDH
Sbjct: 121 LLLNPLLAPPAFASFQTAA----------------KSGWLTSAWTGFLAGCLHTLSGPDH 180

Query: 232 LAALAPLSIGCTRMESAAVGALWGCGHDA------------------------------- 291
           LAALAPLSIG ++MESAAVGALWGCGHDA                               
Sbjct: 181 LAALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGLLFLLLKDRLHIEVLQTWGTRIVGL 240

Query: 292 -----GIMGIREASEVRTPCVVGLDNGECDVGIYETLEKPMVVGKKKKLGFATFATGVVH 351
                G MGI+EASE+  PCV      E D+ +  T ++ + + KKKK+GFATFATGVVH
Sbjct: 241 TLVIIGAMGIKEASEIPEPCVA----LETDISMVSTEKEALPLPKKKKIGFATFATGVVH 300

Query: 352 GLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE 395
           GLQPDALMIVLPALALPSR+AG+AFL+MFLVGTVI+MGSYTAFIGS SEALK++VPRITE
Sbjct: 301 GLQPDALMIVLPALALPSRLAGSAFLIMFLVGTVIAMGSYTAFIGSCSEALKEKVPRITE 360

BLAST of HG10005644.1 vs. TAIR 10
Match: AT4G35080.3 (high-affinity nickel-transport family protein )

HSP 1 Score: 298.5 bits (763), Expect = 7.8e-81
Identity = 196/412 (47.57%), Postives = 247/412 (59.95%), Query Frame = 0

Query: 52  MERLLSSPSPTSLKINPN--PSLILPRFRRIDSVKLNFPLPTRHGLKPLGLISCKNEK-P 111
           MERLL  PS +S  I+P+  PS   P   R+ S  L+F    R   + +  ISC + + P
Sbjct: 1   MERLL-QPSSSSSSISPSKFPSRTSPFLPRLRSSGLSFVSTHRPESRRVSSISCNSSQIP 60

Query: 112 SISLIRCFSSSNSSTDSICQSDSRAGSPNPDFLKHFAG------------------KEKV 171
           S+      +++N+S +   +SD     PNP FL   A                   ++K+
Sbjct: 61  SLYTPIGSNTTNNSFNGSPKSDE--SKPNPGFLTRIATSASEQRKETYEKFVYSLLEKKI 120

Query: 172 KR------------SLFVLSALALILIQPVFAPAAFASFQNAAATTGGPAAAAVGRRLIQ 231
            R            ++ ++SA+A++L+ P+ AP AFASFQ AA                +
Sbjct: 121 PRITKSSNFTLSTGTVILISAVAVLLLNPLLAPPAFASFQTAA----------------K 180

Query: 232 SELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIGCTRMESAAVGALWGCGHDA------- 291
           S  L+SAWTGF AGCLHTLSGPDHLAALAPLSIG ++MESAAVGALWGCGHDA       
Sbjct: 181 SGWLTSAWTGFLAGCLHTLSGPDHLAALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGL 240

Query: 292 -----------------------------GIMGIREASEVRTPCVVGLDNGECDVGIYET 351
                                        G MGI+EASE+  PCV      E D+ +  T
Sbjct: 241 LFLLLKDRLHIEVLQTWGTRIVGLTLVIIGAMGIKEASEIPEPCVA----LETDISMVST 300

Query: 352 LEKPMVVGKKKKLGFATFATGVVHGLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVIS 395
            ++ + + KKKK+GFATFATGVVHGLQPDALMIVLPALALPSR+AG+AFL+MFLVGTVI+
Sbjct: 301 EKEALPLPKKKKIGFATFATGVVHGLQPDALMIVLPALALPSRLAGSAFLIMFLVGTVIA 360

BLAST of HG10005644.1 vs. TAIR 10
Match: AT4G35080.2 (high-affinity nickel-transport family protein )

HSP 1 Score: 249.6 bits (636), Expect = 4.2e-66
Identity = 171/388 (44.07%), Postives = 216/388 (55.67%), Query Frame = 0

Query: 52  MERLLSSPSPTSLKINPN--PSLILPRFRRIDSVKLNFPLPTRHGLKPLGLISCKNEK-P 111
           MERLL  PS +S  I+P+  PS   P   R+ S  L+F    R   + +  ISC + + P
Sbjct: 1   MERLL-QPSSSSSSISPSKFPSRTSPFLPRLRSSGLSFVSTHRPESRRVSSISCNSSQIP 60

Query: 112 SISLIRCFSSSNSSTDSICQSDSRAGSPNPDFLKHFAGKEKVKR------SLFVLSALAL 171
           S+      +++N+S +   +SD     PNP FL   A     +R      ++ ++SA+A+
Sbjct: 61  SLYTPIGSNTTNNSFNGSPKSDE--SKPNPGFLTRIATSASEQRKTLSTGTVILISAVAV 120

Query: 172 ILIQPVFAPAAFASFQNAAATTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDH 231
           +L+ P+ AP AFASFQ AA                +S  L+SAWTGF AGCLHTLSGPDH
Sbjct: 121 LLLNPLLAPPAFASFQTAA----------------KSGWLTSAWTGFLAGCLHTLSGPDH 180

Query: 232 LAALAPLSIGCTRMESAAVGALWGCGHDA------------------------------- 291
           LAALAPLSIG ++MESAAVGALWGCGHDA                               
Sbjct: 181 LAALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGLLFLLLKDRLHIEVLQTWGTRIVGL 240

Query: 292 -----GIMGIREASEVRTPCVVGLDNGECDVGIYETLEKPMVVGKKKKLGFATFATGVVH 351
                G MGI+EASE+  PCV      E D+ +  T ++ + + KKKK+GFATFATGVVH
Sbjct: 241 TLVIIGAMGIKEASEIPEPCVA----LETDISMVSTEKEALPLPKKKKIGFATFATGVVH 300

Query: 352 GLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE 395
           GLQPDALMIVLPALALPSR                             EALK++VPRITE
Sbjct: 301 GLQPDALMIVLPALALPSR-----------------------------EALKEKVPRITE 336