Cucsat.G8047.T3 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G8047.T3
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionRING-type domain-containing protein
Locationctg1556: 3376855 .. 3385103 (-)
RNA-Seq ExpressionCucsat.G8047.T3
SyntenyCucsat.G8047.T3
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGTTGGGTACGGAGGAAAGTTAGAGAAGCGGTATGGATGAGAAATAGAAGGACCAAGCTTCAAAAGATGATGAGATTGATAAGCAAAGTAGATTAAATTGGACCAACCTATGATGTATCGATCATATGGACCGAACCAAGTGAGTTCTTCCAATTTCAACAGAGGAAAATTTGCATTCTTTTGATTTTTCTGGCTTGTATTCCACGTATGATTGGCAGCAACAAATAGATTGTAATAGTAATAAAGGTATCAAATTTCAAGCCTGTTTGAAATTGCGATCGGATTTTGATAATGGAGAGCTGACCTTCTTTCTTCCTTTACCCCTTGATTTTTTCCCCTTTTTCTCTTGTTCGCTCTCTTGCCCTTCTTCCCTCAAGCTTTATAGGGCTCAAATTTCTGCCATTTCTGTTCATCATCACCATCGAAACCCCATCAAGGCTTTCTTTTTTTTTTTTTTTCCTTATTCTTTCATCTTCTTTCCCATCACCCTTCTTTTACTGCCACCATTTTCATCCATTTATCCAATCAAACCCCTCCCTCTTTCACCCCTTTTCATGATTCGCCGGTTTCTTTGACCTTTTTTAATCCGATGATCGTGCCCCACTTGCTTGATTTCTGATTCAGTATTGTTATTGTGTTCTTGTTGTTGCTAGAAGTACTTTTGATTTGATATTGTTATCTGGGTTGTTGCCATGTGATGATTTTCTTTTTCTTTATCTGAGGTTCTCCACGGAGTCTCGTTTGCGGAGAAGGTAATTTCAAGTTCTGTTTCTTTTACTGGTTCTTGATAGTTTTTGTTGCCCATTTCTTGTAGCTTTCAACTTCATTATCTTTAGATTGCTTCTTTTACAAGGAAATTTGTCTTCCCTCTTCATGTTAGGGTCAAATTTTTCGTATGTTGTTGAATTGATTGTCAAGAATGTTATGATGATTAGGGTTAGGCCATTCACCCGAATGGAAGGGTCTGGTAGCGACCCCGACTCTCATAGAGCTGCTTCCACCAGTGGTAGTTCAAGAAGGTTTGGAATGTTAGCTGCCTCAAATATTATTCAGGCTCCGCTTTCTGCACTATTAGAGTATTCGGGTCTTCTTCGTGGCCGTCAAAGTCATCAAGAATCAGAGGCATTGATAAGTGCTCGACTGCCTTCGGGGTTTCGAGACCATCTTCGCAGCCATATTGAAGAATCTTCGGGTGCCACCAATGATGGAGAGGTTTCGATTAGGATAATTGGGGCAGGGGAACCAGAACATGGTAGGGATGGTGCTGGACTAGTAGTTGGGCAAGCGAGGGATGTTAGTGGTCAGAATGCGGTGTCTTTGCAATCTGTTTCAGGGTTGGCTTCTGTGACCTTGGGGTCGGAGCTTAGCCAGGGTGAGTCTGCCAATGAACGCGGTGCAAGAGAGGGTATTCCTGAATCGATAAATGCAGGTGCTGATGGAGATGCCTCGGAGGCAGCTGGAGGAAATAGCAGGGATTCTTCTTATCAGAGATATGACATTCAACAGGCTGCTCGGGGGATTGAGCAGATACTCCCATTTAGTTTTCTTCTACTGGTGGTCTTCATTCGACAGCATTTGCAAGGTACCTGCCTTGACTTTATTCATGATCTTAAATTACTATGGGTTACCAAACATTTATTTACTTCCAATTTTAAGTTGTCATTAATGTGGCTGGATTATGGGCAATCATGAACTACTGCATTGTCTTTATGGAGCAAGCATATGTGCTCTTTAGCTTTATTGTCTTTGATCGTTTTCTATGAGAAAAAAAGCTGAATAAAGTTGAATGAAATTCTTGAAATATCACGGAAATTTTGAAAATCAATGGAATTTAACTTTTATGATCACTTGGTGACTTTCCTATTACCAAATGCAATTGAAAATAGAAAAGCGAACAAACGAACGAGCAGAATTTACAAACTGTCAACTTGGGAAAAAATAATCTTCCAATGTACAATCAATGTTGTATTAACAGTAGTGAAAACTCAACCTATCAAAGTAAACCAAGAATTATACCTCATTTAAATTTCTATAAACTCTAAAATTTTGAAGTATAGTGCATGAAGATAGGAATAGACAAACTGACAAATTACTTAATAATTAAGATCAAACTTTTTTATCCATAAAGATTAATGTGAAGCTCATATCATCTACTTCATCATGTTTTCAAGGGTTAAAATAAAATTTAACTGATGAATTGAGATGTAAACTTCCCTTGTACAAAAATCCATGAACAAGGATATTAGTTCTTTTTTTGTTAGATTCTCAAATTTAGATATAAACTTGTGCATTGTTAGTCATTTTGTTATATTCTTGTATTAGTTTACTTATTTACTTTTAATAATTATATTGTCTTTTTAACTAATTTAATATCAATTTATCAAATCATGATCTTGATGCTAATTATTGGTCATTTTTTGGGGGGTTTAATCCTTTGTGGTTTTGATGCTAACTATTAATGTTTAAGCTTTTTCTGGCTTATATGCAAAATTTACATGTGTTTCCTTGAGAGATATTTAGTTGTATTTATGTGTTTATTATATTGTAGCTCAGATGAACTTAACATCATCTGTATCAAACTAATCCAATTCTCAGAAATTGAAGCATGTAAATATCTTTCCCTTTGATAAGATACCTCAGAGTTTTTTTTTCTTCTCTCTTGAAGAGTTAATTGTGTCAAGGTGATTTAGATGTTGAACAACAAGAACGTAAAAGCCAATAAATTTTTAGTTAAAATTAAAATTTAGAATGAAAGAAAGGAAAAGAAGTAGAATCAAAAGTCTATCACCTTTAATCAATATTTGATTTGACACAGAACTGATTATAACGTAAAGTCAGATATAGAAATATTTAAGAATGCTTTCGTTATTTTGTTTTTACATTTATAAAAGATTAAATTGTTTTAATATTACATGTCTCCACATATATTTCTCTATGATTTCTTCTTCTGGTGTTGATACTTGTGCCCTAAACGTGCAGGCTTCTTTGTGACAATATGGATCGCTGCTTTCATGTACAAGTCAAATGATATTTTGCGAAAACAAACAGCTTTGAAGGTGTGTTAAAGCATATTTTAATGTTTGGGTTCCTTGCAGGCTTTTTTTTGTGTGTGTGTGTCTGTATGTTGGAGTATGAAACAGAAGGAAAGTAGGACGTAAAAATATCCTACGCTGGCATTGGTTAATAGGTTCCTATGTGAACCATAAATGGCTTGATGAGAATGCGTTCAAGCAATGATGACCACCTACTTAGAATTTATTACTCAGTATCCTACAAGTTACTTATTACACGAACACATGTATTATTTTAATGAATAACCAATGTTTCATTGATGGAAATAAATATATTGTAGAGTTAAAAATGGGTTGGTCTAGAGGTAAAAATGGAAATATAGTCTCAATAACTAATTAAAAATATCCTACGCTACCTACCTACGAGTTTCCTTAACACCCAAATGTTGTAGGGTCAGACGGTTGTCTCGTGAGATTAGTTAAGGTGCACAAATGTTGAACTCATGATCGACACCACACCCAAAGGAAATTTCCCAAATGTTGTACCTACGAGTTTCCTTGACACCCAAATGTTGTAGGGTCAGACAGTTGTCTCGTGAGATTAGTCGAGCTGCACATAAGCTGACCAAATTTCTACAAATTACTTGACAATCAAACTTGATAGACTTAGGCAGTTTTCCCATGAGAATAGTCAAGGTGCAAGCTGGCGTTGGGGCACTTGAAAATATAAACAAAACTTTCATTTAATATATGTATAGATTTTATATTTTTATATAAAAACTATTATTATTATTATTTGTTTAAAGAAGTGAAGAAGGTAAGTGCCATTAGGAGACTACAAGAAGGTCTATGTGGATAAGTTAACATCGATGATAAGTCACACTTTATTATTTGGTATCTTGAAGAGTCATGAAAGTTAAAGCCCAAGACATCCTCAAATTTCACCCTTTCCAGCTTAAGCTACCTTAAACAGGAAAACTTTGATGAGCCTAAGCTTCCTTTTATATCAGAGGTTTGGACATGTGTCTGGGGCTACTTTATATTAGAGAAATGTGGGCATCTAGAGCTACAAAACTATTTTTCTTTTGATGCGATAGAGCTACAAAACTATAATATCTACGTCCATGTTTTAGCATCAGCTGCCTCGTCAATTGAAATGGTGTGATCCCCCTTTCCTTAACTCTTTCATGGTGGTTGGATTTTATTTTCCTCTATCTGAAGTTATTCAGCCCATTTCTTAATAGTTTTGCTCCCCGAAATCCCCAGTCAACCCTTATTCCATCACTATAGCTATTTCAATCTCCTTTCAATTTGTAAGGTCTTTTGTAATCTCTCTATTGGGCTCCCCTTTTGTAATTTCATACCATCAACAGAATCATTTGCCTTCTTAAGATTGAGTATAGACCAGGCCATTCACAGGGTAGACTTTCTTATACTTACTCGTTCTGCCCTCCCAGTTAGGGATGGGTTTAAAACTTTTGTCCTGAAACAAATTCGTTTTGATTATCAGAAATAGTATCCTGAATCACTCTACTTGTTAGTTTGGCTAATACCTGATCACTTTATATATGGCAATGACCAATCTTGCTGTACTGTTCCTGTTGTTTTTTCTTTATTCCATTTTTCTAAACATGGGCTTTATTGGTGTTCCTGCAGGGAGAGAGAAAAATTTCTGTTTTGGTTTGCATTTCTCTTGCGTTCACTATTCATGTTATTGGTGTGTACTGGTGGTATCAGAATGATGACCTTTTATATCCGTTGATCATGTGTCCTCCCAGAGCGATTCCTCCTTTCTGGCATGCTATTTTCATCATTATGGTGAATGGTAACCTTCTGTTAAAGTTTTTACTGATTATTAGTGTATGTTCACCGATCATAATCTATACATGTTATATTGACAAAGAAGTGCTTTTACTAACTATGCTAGACTGCCTAGTCACATAATTTTCTTCAAATTATTTATTTTCTCAAATTTTCTCTGACTGATAGTTATTTATTTATTTTAATTGGAGACTTAAAACACTTCATTCAATAAACAAAAAACAAAAGAGGATAACCTAACTGGCAAAGGCCAAGGGTTAAAGAAACACTTTCCTATCAAATTAAAGTGTAACAATTATAAAAAGACTTCTGTTGACTACACTACACCAGTTGAAAGCCTTAAACTTTACAAGATCAGAAAAGTATAAAAAGAATTGACTTAGTATTTTTTTTTAATTTTCTAGATCTACTTGCTTAAAAATGAATACTGCATTTATGTCAGTATCTTACTAGTTACTACTGATTAAATTGTTTTTAGTTATGCCTTTTAGTTTTGCTTTCTAATTTATGAAAGTAAATCACAAACGATTTTTTTGGTAACTAATTAGGTTAAATATATGATTTTGTGTTGGTTTCTCAGACACTTTGGTCCGTCAGGCAGTGATGGTGGTCAAGTGTGTTCTATTGATGTATTACAAGAACAGTAGAGGTCGCAATTATCGTAAACAGGTGGGATAACATAGAAGTTGGCTCTTTTGTATACTTCATGAAAGACTTCTTTTGGATTCAGTAACAAATATCCTTTTATTGAGCTCATTGTTTATTTCTCTTTAAAAAAAGTGGCAAATTTAGAATTTGCAAATGTTTTGTCAGTCTGTGAGTATGCACCTAAAATCCACAGTTTCTCCAGGGTCAAATGCTAACTTTGGTCGAATATTTGCTACTACTGTACCGTGCCTTATTGCCTACTCCTGTTTGGTATCGTTTCTTCTTGAACAAAGAGTATGGGAGTCTATTTTCGTCACTATTGACGGGACTATACTTAACTTTCAAACTCACATCTGTTGTTGAAAAGGTAAGTTTTCTATTACTTTTTGAACTCATTTTTACTATTATATTCTGTCACCAGATGGTCTCTCTGTTATCTCCCTTCCCTGTCTCACTTTTCTTTTTGATGTGGAATTCAGGTGCAATCCTTCTTTGCTGCTTTGAAAGCACTGTCACGTAAAGAGGTGCATTATGGGGCATATGCAACATCAGAGCAGGTAAATTGAATCTCATTTATGCCTTTCTCCAGTCGACTAAACTTTTTTACTGCAGGATGCCATTTATATTTTCCTTTTAATGCATTTTATTTTACTTTTCTGGGAATCCTTGAGTGTCTTTGCTGCATCTTAACTCATCTCAGGACGTTTGCATTTAACGAAATATTGCAAGCATCTTAAATTTGTAGGTTTATTTTCATGAAAAAAAAAAAAAAAAATCAATTGAGTTTCGATAACGCAACCATGGATTCAGTTTTAAGCCATAAAAGCTGTTTTGTGGGATATTTGTTTGGAAGGAAAGCAAAAAACTTTTTAGGAAAGACCCTTGGGGGTGGAATGAAAAGTCTTGCAGGTTTGTGGACTGCAATCCTAGGTTCTCTTTGTATTTAGTCTTATAATGTATTCTCTTCTAGACTTTGTTGGTTTGTTTATTTGTTTTTCTTTGTGTGTTCTCTTTGGATGAGGGATTGAACCATCAATTTTTGAATGGTAATATGTTCTTTGACCACTTGAGTTCTATGCTCATGTTGGTGTCATCCCTTCTAGTTGGGGGCGACTTTCATCATAATTTTGGTCGTTTTCTCTCCTTCAGTAGTCTGTACTCTGTAGTGGTTCCTTCAAACTTTTCCTATAGGCAGATTGGTTGCTGGCGAATTTGTAATTTTTTTAAAAATGATAATAACAACAACAATAATAACAATAATAGAATAATAATATCAATAATAAAAATAACAACAAGAATAACAACAACAAGAATAATAATAAGAATAAGAATAACAACAACAACAACAACAACAAAAAGGAACAGAACAAAATATCCTTGACATCTAACTTGTTATTTGCCTAATTCAGGTCAGCGCAGCAGGTGATTTGTGTGCTATTTGCCAAGAGAAGATGCATGCTCCTATCCTTCTCCGATGTAAACACATATTTTGTGAAGATTGTGTATCAGAATGGTATGTTTATTGCATGAAATTCTCCCATGCTTCATACTCTCACAATCTATATGTTGTACTGATCATTTGCTAAGAGGTTGATTTCAATTTCAGTAATCCAGCACTAAAGGTTTCCTCATCTTATGGTGTGGAGCCTTAAAGTTCGACCATTTGTGATTTCCAATGGTTAAACTATCGCATTTCTGGTTTTTTCAATGTGTGAAACACAACTTACTTACTAATAGTGTTCAATTGTCGAATGTTATAGACATAGCTTAGTGGATGAGAAATTCTGCGGAGTTGGGACTTGATAACAAGGGTAATATGGTAATTAGTGGGGGTAGTTTTTGTAAGGGAATAATTGTTGTAAGTAGATGGATAGAGATGGGAGAAGGCAATCCATCATCGGGGTGGTTTAATCTTGAGCAAACTTAGATGATGTACTTCGAATTACTTGGTATTTTGATTTTCGAGTCTTGTTATCTTGTTCCAAACTAGATTTCAAACAAAACTAGCATTTTTTGTCCTACTTCTATTAACCAGGACTTCGGAACTAAAACGTCCAAAGGAAGGAGAATGGGGGCAAATAAATGCCAACTTACCTACCTTTAGTGTTCTTAATATATTGTTCACTTTGATACCAATTTTGGACTGGTTGGTGCTATGTGCAGGTTTGAGCGGGAACGGACATGTCCATTGTGTAGGGCGTTGGTTAAACCCGCAGATCTGAGGTCATTTGGTGATGGATCAACAAGTTTGTTTTTCCAGGTTTTCTAAGCCATTTACTTCAAAGCCCCTCAGAAGGCTTTGTAGTTTGAAGAGGTGAAGGGAAGCCAGCCAATTGCATTTGTTTACCCCTACACAAAATTGTACATCTAACTAAATACTGTGTAGCTGCTGCCAATTGAGGAAGCAAAAGACTGGGGCTTGTGAAGTTGGAAAGGCAAATTCAAAATCGACATTTATACATATTATAGTCCTTTTTGTGCTACAGCAAAGAACGGTGGATGTTCGAGAGGAGTTTAACCCTTCTATGAAAATTTTATGAATTGTGATTCTGGGGTGTAAATTTATTAGCAAAGCCAGTTTGCTGTGTTGTCGATTTTATAATTTAGTCCAAATGCATATAATGAGAATGTTTTGGACTCTAGTAATTGTTATATACACATACACAGTGTCAGACATGTCTTTTGGCTCTTCAGTCTGTGCTTTTGTTTGATTTGAAAATTAAGAAAGGAAGTGAATATGATGAATTTTTAGAATTGGATATCTTTTT

Coding sequence (CDS)

ATGCTAACTTTGGTCGAATATTTGCTACTACTGTACCGTGCCTTATTGCCTACTCCTGTTTGGTATCGTTTCTTCTTGAACAAAGAGTATGGGAGTCTATTTTCGTCACTATTGACGGGACTATACTTAACTTTCAAACTCACATCTGTTGTTGAAAAGGTGCAATCCTTCTTTGCTGCTTTGAAAGCACTGTCACGTAAAGAGGTGCATTATGGGGCATATGCAACATCAGAGCAGGTCAGCGCAGCAGGTGATTTGTGTGCTATTTGCCAAGAGAAGATGCATGCTCCTATCCTTCTCCGATGTAAACACATATTTTGTGAAGATTGTGTATCAGAATGGTTTGAGCGGGAACGGACATGTCCATTGTGTAGGGCGTTGGTTAAACCCGCAGATCTGAGGTCATTTGGTGATGGATCAACAAGTTTGTTTTTCCAGGTTTTCTAA

Protein sequence

MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAALKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPADLRSFGDGSTSLFFQVF
Homology
BLAST of Cucsat.G8047.T3 vs. ExPASy Swiss-Prot
Match: Q96EX2 (RING finger and transmembrane domain-containing protein 2 OS=Homo sapiens OX=9606 GN=RNFT2 PE=2 SV=2)

HSP 1 Score: 112.8 bits (281), Expect = 3.2e-24
Identity = 58/147 (39.46%), Postives = 90/147 (61.22%), Query Frame = 0

Query: 4   LVEYLLLLYRALLPTPVWYRFFLNKEYGSLF--SSLLTGLYLTFKLTSVVEKVQSFFAAL 63
           ++E L  L+R+L+P  +WY++ +  +  + +    +L  LY   K   +  +V     AL
Sbjct: 300 VIEELSQLFRSLVPIQLWYKYIMGDDSSNSYFLGGVLIVLYSLCKSFDICGRVGGVRKAL 359

Query: 64  KALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERTC 123
           K L   + +YG  AT +Q + AGD+CAICQ +   P++L C+H+FCE+C+  W +RERTC
Sbjct: 360 KLLCTSQ-NYGVRATGQQCTEAGDICAICQAEFREPLILLCQHVFCEECLCLWLDRERTC 419

Query: 124 PLCRALVKPADLRSFGDGSTSLFFQVF 149
           PLCR+ V    LR + DG+TS  FQV+
Sbjct: 420 PLCRS-VAVDTLRCWKDGATSAHFQVY 444

BLAST of Cucsat.G8047.T3 vs. ExPASy Swiss-Prot
Match: Q5RAG4 (RING finger and transmembrane domain-containing protein 2 OS=Pongo abelii OX=9601 GN=RNFT2 PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 4.7e-23
Identity = 57/147 (38.78%), Postives = 89/147 (60.54%), Query Frame = 0

Query: 4   LVEYLLLLYRALLPTPVWYRFFLNKEYGSLF--SSLLTGLYLTFKLTSVVEKVQSFFAAL 63
           ++E L  L+R+L+P  +WY++ +  +  + +    +L  LY   K   +  +V     AL
Sbjct: 300 VIEELSQLFRSLVPIQLWYKYIMGDDSSNSYFLGGVLIVLYSLCKSFDICGRVGGVRKAL 359

Query: 64  KALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERTC 123
           K L   + +YG  AT +Q + AGD+CAICQ +   P++L C+H+FCE+C+  W +RERTC
Sbjct: 360 KLLCTSQ-NYGVRATGQQCTEAGDICAICQAEFREPLILLCQHVFCEECLCLWLDRERTC 419

Query: 124 PLCRALVKPADLRSFGDGSTSLFFQVF 149
           PL R+ V    LR + DG+TS  FQV+
Sbjct: 420 PLSRS-VAVDTLRCWKDGATSAHFQVY 444

BLAST of Cucsat.G8047.T3 vs. ExPASy Swiss-Prot
Match: Q5M7Z0 (E3 ubiquitin-protein ligase RNFT1 OS=Homo sapiens OX=9606 GN=RNFT1 PE=1 SV=2)

HSP 1 Score: 107.5 bits (267), Expect = 1.4e-22
Identity = 57/150 (38.00%), Postives = 88/150 (58.67%), Query Frame = 0

Query: 4   LVEYLLLLYRALLPTPVWYRFFLN-KEYGSL----FSSLLTGLYLTFKLTSVVEKVQSFF 63
           L+E L   YR  +P PVW+R+ ++  E+G++       LL  LYL  KL      +++F 
Sbjct: 288 LLEELCQYYRTFVPIPVWFRYLISYGEFGNVTRWSLGILLALLYLILKLLEFFGHLRTFR 347

Query: 64  AALKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERE 123
             L+ +   +  YG  A+  Q S   D+C+ICQ +   PILL C+HIFCE+C++ WF RE
Sbjct: 348 QVLR-IFFTQPSYGVAASKRQCSDVDDICSICQAEFQKPILLICQHIFCEECMTLWFNRE 407

Query: 124 RTCPLCRALVKPADLRSFGDGSTSLFFQVF 149
           +TCPLCR ++    +  + DG+TS   Q++
Sbjct: 408 KTCPLCRTVISD-HINKWKDGATSSHLQIY 435

BLAST of Cucsat.G8047.T3 vs. ExPASy Swiss-Prot
Match: Q3UF64 (RING finger and transmembrane domain-containing protein 2 OS=Mus musculus OX=10090 GN=Rnft2 PE=2 SV=2)

HSP 1 Score: 106.7 bits (265), Expect = 2.3e-22
Identity = 56/147 (38.10%), Postives = 88/147 (59.86%), Query Frame = 0

Query: 4   LVEYLLLLYRALLPTPVWYRFFLNKEYGSLF--SSLLTGLYLTFKLTSVVEKVQSFFAAL 63
           ++E L  L+R+L+P  +WY++ +  +  + +    +L  LY   K   +  +V     AL
Sbjct: 301 VIEELSQLFRSLVPIQLWYKYIMGDDSSNSYFLGGVLIVLYSLCKSFDICGRVGGLRKAL 360

Query: 64  KALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERTC 123
           K L   + +YG  AT +Q + AG +CAICQ +   P++L C+H+FCE+C+  W +RERTC
Sbjct: 361 KLLCTSQ-NYGVRATGQQCTEAGAVCAICQAEFRDPMILLCQHVFCEECLCLWLDRERTC 420

Query: 124 PLCRALVKPADLRSFGDGSTSLFFQVF 149
           PLCR+ V    LR + DG+TS   QV+
Sbjct: 421 PLCRS-VAVDTLRCWKDGATSAHLQVY 445

BLAST of Cucsat.G8047.T3 vs. ExPASy Swiss-Prot
Match: Q6NTV1 (E3 ubiquitin-protein ligase RNFT1 OS=Xenopus laevis OX=8355 GN=rnft1 PE=2 SV=2)

HSP 1 Score: 106.3 bits (264), Expect = 3.0e-22
Identity = 59/151 (39.07%), Postives = 87/151 (57.62%), Query Frame = 0

Query: 5   VEYLLLLYRALLPTPVWYRFFLNKEYGSL-------FSSLLTGLYLTFKLTSVVEKVQSF 64
           +E L   Y  L+ TPVW+R+ +  +YG+L       F  LL  LYL  K+  +  + ++ 
Sbjct: 270 LEELAQCYCTLVSTPVWFRYLI--DYGNLDSGAEWHFGILLALLYLILKILIIFGQRKTS 329

Query: 65  FAALKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFER 124
              L+ L   + +YG  AT  Q S A  +CAICQ +   PI L C+H+FCE+C+S WF +
Sbjct: 330 SDCLR-LFLSQPNYGTTATKRQCSEADGMCAICQAEFTKPIALICQHVFCEECISSWFNK 389

Query: 125 ERTCPLCRALVKPADLRSFGDGSTSLFFQVF 149
           E+TCPLCR L+       + DG+TSL  ++F
Sbjct: 390 EKTCPLCRTLISNHS-HKWKDGATSLQLRIF 416

BLAST of Cucsat.G8047.T3 vs. NCBI nr
Match: KHN40596.1 (RING finger and transmembrane domain-containing protein 2 [Glycine soja])

HSP 1 Score: 288 bits (736), Expect = 1.50e-96
Identity = 143/148 (96.62%), Postives = 148/148 (100.00%), Query Frame = 0

Query: 1   MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA 60
           MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSL+TGLYLTFKLTSVVEKVQSFFAA
Sbjct: 85  MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLMTGLYLTFKLTSVVEKVQSFFAA 144

Query: 61  LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 120
           +KALSRKE+HYGAYATSEQV+AAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT
Sbjct: 145 VKALSRKEMHYGAYATSEQVNAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 204

Query: 121 CPLCRALVKPADLRSFGDGSTSLFFQVF 148
           CPLCRALVKPADLRSFGDGSTSLFFQ+F
Sbjct: 205 CPLCRALVKPADLRSFGDGSTSLFFQLF 232

BLAST of Cucsat.G8047.T3 vs. NCBI nr
Match: KAG6595535.1 (RING finger and transmembrane domain-containing protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 290 bits (742), Expect = 3.78e-96
Identity = 146/147 (99.32%), Postives = 147/147 (100.00%), Query Frame = 0

Query: 1   MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA 60
           MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA
Sbjct: 154 MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA 213

Query: 61  LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 120
           LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT
Sbjct: 214 LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 273

Query: 121 CPLCRALVKPADLRSFGDGSTSLFFQV 147
           CPLCRALVKPADLRSFGDGSTSLFFQ+
Sbjct: 274 CPLCRALVKPADLRSFGDGSTSLFFQL 300

BLAST of Cucsat.G8047.T3 vs. NCBI nr
Match: XP_030519052.1 (RING finger and transmembrane domain-containing protein 2-like [Rhodamnia argentea])

HSP 1 Score: 284 bits (727), Expect = 4.53e-96
Identity = 140/148 (94.59%), Postives = 146/148 (98.65%), Query Frame = 0

Query: 1   MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA 60
           MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSL+TGLYLTFKLTSVVEKVQ FF A
Sbjct: 25  MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLMTGLYLTFKLTSVVEKVQFFFTA 84

Query: 61  LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 120
           LKALSRK++HYGAYAT+EQV+AAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT
Sbjct: 85  LKALSRKDIHYGAYATAEQVNAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 144

Query: 121 CPLCRALVKPADLRSFGDGSTSLFFQVF 148
           CPLCRALVKPADLRSFGDGSTSLFFQ+F
Sbjct: 145 CPLCRALVKPADLRSFGDGSTSLFFQIF 172

BLAST of Cucsat.G8047.T3 vs. NCBI nr
Match: KAG1371461.1 (putative RING finger and transmembrane domain-containing protein 2 [Cocos nucifera])

HSP 1 Score: 285 bits (730), Expect = 8.47e-96
Identity = 143/148 (96.62%), Postives = 146/148 (98.65%), Query Frame = 0

Query: 1   MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA 60
           MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSL TGLYLTFKLTSVVEKVQSFFAA
Sbjct: 74  MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLTTGLYLTFKLTSVVEKVQSFFAA 133

Query: 61  LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 120
           LKALSRKEVHYG+YAT+EQV AAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT
Sbjct: 134 LKALSRKEVHYGSYATTEQVIAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 193

Query: 121 CPLCRALVKPADLRSFGDGSTSLFFQVF 148
           CPLCRALVKPADLRSFGDGSTSLFFQ+F
Sbjct: 194 CPLCRALVKPADLRSFGDGSTSLFFQLF 221

BLAST of Cucsat.G8047.T3 vs. NCBI nr
Match: MBA0681229.1 (hypothetical protein [Gossypium aridum] >MBA0797352.1 hypothetical protein [Gossypium harknessii])

HSP 1 Score: 287 bits (734), Expect = 1.29e-95
Identity = 141/148 (95.27%), Postives = 148/148 (100.00%), Query Frame = 0

Query: 1   MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA 60
           MLTLVEY++LLYRALLPTPVWYRFFLNK+YGSLFSSL+TGLYLTFKLTSVVEKVQSFFAA
Sbjct: 128 MLTLVEYMMLLYRALLPTPVWYRFFLNKDYGSLFSSLMTGLYLTFKLTSVVEKVQSFFAA 187

Query: 61  LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 120
           LKALSRKEVHYGAYATSEQV+AAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT
Sbjct: 188 LKALSRKEVHYGAYATSEQVNAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 247

Query: 121 CPLCRALVKPADLRSFGDGSTSLFFQVF 148
           CPLCRALVKPADLRS+GDGSTSLFFQ+F
Sbjct: 248 CPLCRALVKPADLRSYGDGSTSLFFQIF 275

BLAST of Cucsat.G8047.T3 vs. ExPASy TrEMBL
Match: A0A2P2LNZ7 (RING-type domain-containing protein OS=Rhizophora mucronata OX=61149 PE=4 SV=1)

HSP 1 Score: 288 bits (737), Expect = 9.74e-97
Identity = 142/148 (95.95%), Postives = 147/148 (99.32%), Query Frame = 0

Query: 1   MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA 60
           MLTLVEYL+LLYRALLPTPVWYRFFLNKEYGSLFSSL+TGLYLTFKLTSVVEKVQSFFAA
Sbjct: 104 MLTLVEYLMLLYRALLPTPVWYRFFLNKEYGSLFSSLMTGLYLTFKLTSVVEKVQSFFAA 163

Query: 61  LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 120
           LKALSRKE+HYG YATSEQV+AAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT
Sbjct: 164 LKALSRKEIHYGVYATSEQVNAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 223

Query: 121 CPLCRALVKPADLRSFGDGSTSLFFQVF 148
           CPLCRALVKPADLRSFGDGSTSLFFQ+F
Sbjct: 224 CPLCRALVKPADLRSFGDGSTSLFFQIF 251

BLAST of Cucsat.G8047.T3 vs. ExPASy TrEMBL
Match: A0A7J8X353 (RING-type domain-containing protein OS=Gossypium aridum OX=34290 GN=Goari_023047 PE=4 SV=1)

HSP 1 Score: 287 bits (734), Expect = 6.23e-96
Identity = 141/148 (95.27%), Postives = 148/148 (100.00%), Query Frame = 0

Query: 1   MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA 60
           MLTLVEY++LLYRALLPTPVWYRFFLNK+YGSLFSSL+TGLYLTFKLTSVVEKVQSFFAA
Sbjct: 128 MLTLVEYMMLLYRALLPTPVWYRFFLNKDYGSLFSSLMTGLYLTFKLTSVVEKVQSFFAA 187

Query: 61  LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 120
           LKALSRKEVHYGAYATSEQV+AAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT
Sbjct: 188 LKALSRKEVHYGAYATSEQVNAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 247

Query: 121 CPLCRALVKPADLRSFGDGSTSLFFQVF 148
           CPLCRALVKPADLRS+GDGSTSLFFQ+F
Sbjct: 248 CPLCRALVKPADLRSYGDGSTSLFFQIF 275

BLAST of Cucsat.G8047.T3 vs. ExPASy TrEMBL
Match: A0A7J9GIG0 (RING-type domain-containing protein OS=Gossypium harknessii OX=34285 GN=Gohar_008061 PE=4 SV=1)

HSP 1 Score: 287 bits (734), Expect = 6.23e-96
Identity = 141/148 (95.27%), Postives = 148/148 (100.00%), Query Frame = 0

Query: 1   MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA 60
           MLTLVEY++LLYRALLPTPVWYRFFLNK+YGSLFSSL+TGLYLTFKLTSVVEKVQSFFAA
Sbjct: 128 MLTLVEYMMLLYRALLPTPVWYRFFLNKDYGSLFSSLMTGLYLTFKLTSVVEKVQSFFAA 187

Query: 61  LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 120
           LKALSRKEVHYGAYATSEQV+AAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT
Sbjct: 188 LKALSRKEVHYGAYATSEQVNAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 247

Query: 121 CPLCRALVKPADLRSFGDGSTSLFFQVF 148
           CPLCRALVKPADLRS+GDGSTSLFFQ+F
Sbjct: 248 CPLCRALVKPADLRSYGDGSTSLFFQIF 275

BLAST of Cucsat.G8047.T3 vs. ExPASy TrEMBL
Match: A0A6J1EDR3 (E3 ubiquitin-protein ligase RNFT1-like OS=Cucurbita moschata OX=3662 GN=LOC111432304 PE=4 SV=1)

HSP 1 Score: 293 bits (750), Expect = 9.30e-96
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0

Query: 1   MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA 60
           MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA
Sbjct: 320 MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA 379

Query: 61  LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 120
           LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT
Sbjct: 380 LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 439

Query: 121 CPLCRALVKPADLRSFGDGSTSLFFQVF 148
           CPLCRALVKPADLRSFGDGSTSLFFQVF
Sbjct: 440 CPLCRALVKPADLRSFGDGSTSLFFQVF 467

BLAST of Cucsat.G8047.T3 vs. ExPASy TrEMBL
Match: A0A0A0L088 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G337310 PE=4 SV=1)

HSP 1 Score: 293 bits (750), Expect = 1.04e-95
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0

Query: 1   MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA 60
           MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA
Sbjct: 324 MLTLVEYLLLLYRALLPTPVWYRFFLNKEYGSLFSSLLTGLYLTFKLTSVVEKVQSFFAA 383

Query: 61  LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 120
           LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT
Sbjct: 384 LKALSRKEVHYGAYATSEQVSAAGDLCAICQEKMHAPILLRCKHIFCEDCVSEWFERERT 443

Query: 121 CPLCRALVKPADLRSFGDGSTSLFFQVF 148
           CPLCRALVKPADLRSFGDGSTSLFFQVF
Sbjct: 444 CPLCRALVKPADLRSFGDGSTSLFFQVF 471

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q96EX23.2e-2439.46RING finger and transmembrane domain-containing protein 2 OS=Homo sapiens OX=960... [more]
Q5RAG44.7e-2338.78RING finger and transmembrane domain-containing protein 2 OS=Pongo abelii OX=960... [more]
Q5M7Z01.4e-2238.00E3 ubiquitin-protein ligase RNFT1 OS=Homo sapiens OX=9606 GN=RNFT1 PE=1 SV=2[more]
Q3UF642.3e-2238.10RING finger and transmembrane domain-containing protein 2 OS=Mus musculus OX=100... [more]
Q6NTV13.0e-2239.07E3 ubiquitin-protein ligase RNFT1 OS=Xenopus laevis OX=8355 GN=rnft1 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
KHN40596.11.50e-9696.62RING finger and transmembrane domain-containing protein 2 [Glycine soja][more]
KAG6595535.13.78e-9699.32RING finger and transmembrane domain-containing protein 2, partial [Cucurbita ar... [more]
XP_030519052.14.53e-9694.59RING finger and transmembrane domain-containing protein 2-like [Rhodamnia argent... [more]
KAG1371461.18.47e-9696.62putative RING finger and transmembrane domain-containing protein 2 [Cocos nucife... [more]
MBA0681229.11.29e-9595.27hypothetical protein [Gossypium aridum] >MBA0797352.1 hypothetical protein [Goss... [more]
Match NameE-valueIdentityDescription
A0A2P2LNZ79.74e-9795.95RING-type domain-containing protein OS=Rhizophora mucronata OX=61149 PE=4 SV=1[more]
A0A7J8X3536.23e-9695.27RING-type domain-containing protein OS=Gossypium aridum OX=34290 GN=Goari_023047... [more]
A0A7J9GIG06.23e-9695.27RING-type domain-containing protein OS=Gossypium harknessii OX=34285 GN=Gohar_00... [more]
A0A6J1EDR39.30e-96100.00E3 ubiquitin-protein ligase RNFT1-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A0A0L0881.04e-95100.00RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G337310 P... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 87..124
e-value: 1.7E-7
score: 40.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 87..125
score: 13.595753
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 85..128
e-value: 4.6E-11
score: 42.4
NoneNo IPR availablePANTHERPTHR15860:SF19RING/U-BOX SUPERFAMILY PROTEINcoord: 1..148
NoneNo IPR availableCDDcd16532RING-HC_RNFT1_likecoord: 86..125
e-value: 2.24918E-25
score: 89.2653
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 86..130
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 61..135
e-value: 4.0E-19
score: 69.8
IPR044235RING finger and transmembrane domain-containing protein 1/2PANTHERPTHR15860UNCHARACTERIZED RING FINGER-CONTAINING PROTEINcoord: 1..148
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 102..111

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G8047Cucsat.G8047gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G8047.T3.E1Cucsat.G8047.T3.E1exon
Cucsat.G8047.T3.E2Cucsat.G8047.T3.E2exon
Cucsat.G8047.T3.E3Cucsat.G8047.T3.E3exon
Cucsat.G8047.T3.E4Cucsat.G8047.T3.E4exon
Cucsat.G8047.T3.E5Cucsat.G8047.T3.E5exon
Cucsat.G8047.T3.E6Cucsat.G8047.T3.E6exon
Cucsat.G8047.T3.E7Cucsat.G8047.T3.E7exon
Cucsat.G8047.T3.E8Cucsat.G8047.T3.E8exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G8047.T3.C4Cucsat.G8047.T3.C4CDS
Cucsat.G8047.T3.C3Cucsat.G8047.T3.C3CDS
Cucsat.G8047.T3.C2Cucsat.G8047.T3.C2CDS
Cucsat.G8047.T3.C1Cucsat.G8047.T3.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G8047.T3Cucsat.G8047.T3-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1904294 positive regulation of ERAD pathway
biological_process GO:0016310 phosphorylation
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0016301 kinase activity
molecular_function GO:0046872 metal ion binding