Cucsat.G4655.T6 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G4655.T6
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionRhamnogalacturonan endolyase
Locationctg1227: 1346142 .. 1351743 (+)
RNA-Seq ExpressionCucsat.G4655.T6
SyntenyCucsat.G4655.T6
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAATCTCACTTATATAACTATCCTCAACTTGCCGGAAACAAGACATTACTTCTGTGAGAAAGAATTGGCTTCTCTCTCAAACAGTTAGTCGAAAGCCGCTGACATGCCGCCACTGGGTGTGCAGCTCTTTATTACAGAACGTCAGGTAAGTCCTTCCCTCCCTCTCTGCATCTCTCTCTCTGTCCACCTCTCTATTATTAAAGCGGTGAAATGTGCAGGTGACAATGGACAATGGCATAGTTCAAGTCACGTTATCAAACCCAGATGGCATAGTAACTGGAATTCGATACGGTGGAGTTGACAACGTGCTCGAAGTCCTAAACGATGAATCTAACCGAGGGTATATATTTTGAAGGAAATACTTGCCTATACACTTTATTCAGTTGATGGTTCGACGACTTTCTTATTTGGCTTATTCTTCGTTATTCAAGAACATCAGCAGGATCGATGCAATTTGAAGAATAAAAATAAGTCACGTTAGAAAGTCATTAAGAGTTCCGACTGATGATGATGGGTGTTTAGAAACGTATTATTCTACAAAGAACATCATTTCGATTTCATTAATTGTGTCCATGCATGAGTTAGAGGCTATATTACAAAAATTATGCTCCTAAACTTTCTCATTTGTTTAAAAAATACTCTTACTCTTTTAAAAGTTGTAATATTAGCCTTGACCTTTCATTTGTCTCAGAAACTAACTTTAAAGTAGAAATTCTTTGAAGCTTTTAATGGAAACTGAGATATGAAATGTGTGTGTTGCAGTGTACGAGTTATCACTGTATGCTGTGTTATGTTGTCTTTTTTGTCTAATGTGACATGTCCATTCCAATGGAAGAATGTGATATTTTTTGAAATGAATGCCAGTTTGGAGTTAAGTTTTGTAATTTAGTCTAAGTTAAATGTTTGTCGTTTGTAATTTCACTTTTCATGCAGGTATTGGGACCTCGTGTGGAGCTCATCATCGTCCACAACTGGATTGTTTGATGTGTATGTATTCGCTTCTCTGCTTTTTTTTCTTTTCAAGTTGGATTTCTTACAGATTTTCGGGCCTCTAAGCTTTTTCCTTGGTTAATACGTGTAACTTTGTTTTGTAGGATAAAGGCTACAGGTTTCGAGGTCATAGTGGAAAATGAAGAACAAGTGGAACTCTCCTTTACAAGAACATGGGATCTGTCTCTCGAGGGAAAACTTGTGCCTTTAGAGATTGATAAAAGGTGACATTGAATTCATGCAGTCTGCTAAAATTAAAGTTGCAAACCTCTTTCTGATGAGTGGATTTGTAGCTTTGTAGGTTTATAATGCTCCGGGGTTCGTCTGGGTTCTACTCTTACGCTATTTACCAACACCTGGAAGAATGGCCTGGTTTCAACCTTGGTGAAACTAGACTCGCCTTCAAACTCAGAAAAGACAAGTATCAAATTCTCAACAATATTCCTTTTTTAATGCTTAATTTAAAGCATTCGTGTGGACTTTTTAGTATTATTCTGGACACGGTCATATATGGTTGGACACAAATATTCTTTTGTAGGTTCCACTATATGGCCATGGCAGACAACAGACAGAGGTACATGCCACTTCCAGATGACCGTTCTCCGCCTAGAGGTCGTGCCCTGGCATATCCAGAGGCTGTCCTGCTCGTCGACCCCATCGAACCTGAGCTCCAGGGAGAGGTCTATTATCGAATCTCTTCTTTATAAGCCTCTAATTTTGTTTAAAGCAAAAGAAAAAAAGAAGTGTCATAAGATCAGCGAAATCACCGCTCAGAGCAAAACTCAAGTTAATTATTAATTTATATTCTTTATACTTTTCTTCTCTTTACATAAAAAATGTGCACAAAACTCAACATGATTATCAATATTAATTATCGTATACTGTGTTAAATCGTTACTTAATAGATAAGAGCAATTTTTTTCCTTACAATGTGGGACTACAAATTTAAATATCTGATCTTTATAATTCTACAACCACGTATATGATTAAATTTATTGACGGATTCAATTATGGGCAATGATCTATTCGATCATCATTAGACCGAAAATTAAGCCTATGACTACACTACAATTTTCACCAAAACCAATCTTTTATTATTTTCTTTTTTATAGAGAAAACCAGAATCATGCTAACGTAAATGAGTTTTGATTCCTCTAATTTCATATGGACTGTAAAGGTGGATGACAAATATCAGTACTCATGCGACAACAAAGACAGCGGCGTCCATGGATGGATATCCACCGATCCGGCAGTCGGATTCTGGCTAATCTCCCCGAGCAATGAGTTCCGATCCGGTGGACCCGTTAAACAGAACCTAACTTCACACGTCGGCCCAACCACCCTAGCTGTAAGTGATTCTTCGTTCATTCAGCCATCAATCGTTATCCTTGGCTTTGATGTTCGATATAATTGGAATTAAGCCAGATTATACATTTACCTTAACTATGGAATCAGGTTTTTATAAGCGCACATTACGCGGGAGAGGATCTGGTGCCGAAAATCGGAGCCGGCGAGGCGTGGAAGAAAGTTTTCGGTCCTGTGTTCATCTATCTCAACTCCACTTATGATTCCTTCGACGCACTTCAGCTCTGGGAAGATGCCAAAGCACAGGTTTCTTCTATTTTTCTTCATTTCCATTCAATGACAATTCTTTTCTCGCGTCTTCTTTACCCACATCCAAATTTCTGGGTTTTTTTTTTTTTTTTTTGAATTATTCCAGTTCCTAAGATGGGTAATTTGACCTGTCTACTATCTTTTTACACAAAAAATAGACAATCTATTATTATATATATGTGTTTGCCACACGGTGTAAGTTTATTAAAAAAATTATTTGGACTTTACCAAGATAAGCGGCTGCCTGCAATCGAACCTGCGCCTAAGTTTTTCTTCTTGTTTGTTCATCAATTATTTTAAGTTCCAACATATCCATAAAATTTGTAGTACTTTCAATTTTGTCTCAGTACTAGTAAAGGTGTATGGATTAAATTTGAAAAAATAGATTTTAGTCAAAAGTTTTGGCAAGAAAACTTTTCCTGTGACTTACTTTGAGTTACTCTTAAAATCAAATAAAATTTAAGAAATATTTTTCCCCATGAAAAGGCAGTGATCGAAGTCCAGAGTTGGCCCTACAGCTTCCCTGCTTCGGATGATTTTCCAAGGTCAAACCAACGTAGTAGTATCAGTGGTAAACTATTAGTCAAGGATAGGTACACAATTGCCTCAACATTATATTTAAAATGTTTATGATGGAAATTCTGGTGAATTTTAGTGCTTCATCCAAAACTAATTGGCTAATTTAAATATCGCGACTTGATTTTAGCTACGTCAATGAAGACTTGATACCAGCAGACGGTGCGTTTGTTGGCCTGGCTCCACCAGGAGACGTAGGATCATGGCAACGAGAGAGCAAGGTAAAATAATTTAATTTTCTTGTTTAACCAATAAATTTTATAGAATTATTTTGATGGTATCGTCCAAAATTTTTTAGGGTTACCAATTTTGGAGTAGAGCAGATGAAAGTGGAAATTTCTCCATCGATGGCGTGAGGCCGGGCGACTACAATCTGTATGCATGGGTCCCCGGTTTTATTGGAGATTATCGTTATGATGCTCTCATCAACCTTACAGAAGGTTCCAATTTTCTTACTCTATGGCAATAAAATTGGTTAGAATCTCTAGTTTAGAAAAGTTAATGGGACAATCTGATGTTTCTATTGAAAAATATATAAACATTTCTCTTCCAAATTTCCAATGGACCTTAGCGTAAATGCATTTTGGTGTCTGAGTTTGCATATTCAATATTCAAACTTCAGTTTGACCTCTTCCATGAGGTTATTAACTTTAAATTTTAATTGATCTAAGAGAAAATTAATTAATTGTTGCCAAAATAAGTATAATTTAATTATAAAAAATGTTCAAATCAACCAACATATTTACTAGATATAACAAAATTTTAGATCTAACTAGTTAATACTAGTTACTATCGAATTAATTAGAAATCCTCTCATGGTTGAAAAAAATCTCTCACTCATAGTGGTATAAAGTTGAATAGATCCAAGATTCATTTGTTGGGTTGTTTTGTACCAATCCATTTCTATCTGTACTATATGTCTGTTTTTTTCTTCTAAATTTGGACAAGCAATTATAGGTTAATTTTGTTAATATACGATAATTTATGTAGGCAGTGAGGTGGACATGGGAGACATAACATACGAACCTCCGAGACAAGGAGCCACACTATGGGAAATAGGAATCCCAGATCGCTCCGCAGCAGAGTTCTACGTTCCAGATCCAAATCCCAACTACGTCAATAAGCTCTACCTTAACCATCCTGATAAGTAAATGAACCTACTTCATATTTCAAGCCCACTGATTTTTCATTATTCCCCTTTAATCGAATCAAAAGATTTGGTGAATCCTTGTACCAAGTCTTGATGAGATATCACTTTGATAAAATTACTCCCACGTTCTTAATTAATCTGATAAAGGATTTGACAGGTTTCGGCAGTATGGCCTATGGGAAAGATACAGTGAATTGTTTCCCGACTCAGATTTGGTATATACCGTTGGCGTTAGTGACTACACCAAGGATTGGTTCTTTGCTCAGGTCCCCAGGTGTGTGCTTCATTAATTTTTCCGATCATCCAGAAATAATTTCAAACTTGTTTGGTTTGATTATTAAAATGGAATTCCATAATTAAACTGATAAACCAAAAACCTGGGAGATACGAACAGTATAGTATCCATCTGTTTGACAGCTTCTTTTCACCTACAGGAAGATGGAAAATGGGACATATAAGGGAACAACGTGGCAAGTGAAGTTCGAGCTAGACGACGTGGACAACCAAGCAACTTACAAGCTTCGGGTTGCATTGGCCTCCGTTTCTCTCGCAGAATTGCAGGTATACCAAATTTCAACGTTTCCTTTTCCTAAAAAGTTGAATTGCCAAATCATATTGTTTCTAAACGTTGCACTTTCTTAATAGCAGTAAGATGTTTATCTGATTAGTTAGAAGCCTAGTAACAGTGAATGAAAGGAAAGTTTTGAGTTTTGAAGGTTGGAGTTAACGAGGCCAAAGCAAAGGCAGTGTTCTCGAGTGGACTTATCGGGAGGGACAATTCGATTGCAAGACACGGCATTCACGGCCTATATTGGGTTTACAACATTGACATTCCTGGAAATCGGCTGGTCACCGGCAATAACACCATCTTCTTCACCCAACCCAGATGCACAGGCCCTTTCCAAGCGATTATGTACGATTACATTCGTCTGGAAGCTCCTCCACCTCCATCCTCCGCCCATCCTTTGGAATAATATTCCCCTACCACACGTCCTTCCCTTCCACATCCTTAATTTCCACCATTTGCTTTCTTGTTTCGTTTTCTTTATACACAATCAATTTCGCTAACTTAAAAACTGTACTCGTAACCAGGGTAGGATCTTTCCTAAGGGGTTTAGAATGAGATTCAGACTTTTAGAATTGATAAAGTTCATAATAAAACCTATGTCGTGAAACTACTACAATCTCATGTGTCGTGAAAAATGCAAAACCAAAAAACAATTTTATAGACATCATATTATTCAGTACAAAATATGTACAAAATATTGGGA

Coding sequence (CDS)

ATGCCGCCACTGGGTGTGCAGCTCTTTATTACAGAACGTCAGGTGACAATGGACAATGGCATAGTTCAAGTCACGTTATCAAACCCAGATGGCATAGTAACTGGAATTCGATACGGTGGAGTTGACAACGTGCTCGAAGTCCTAAACGATGAATCTAACCGAGGGTATTGGGACCTCGTGTGGAGCTCATCATCGTCCACAACTGGATTGTTTGATGTGATAAAGGCTACAGGTTTCGAGGTCATAGTGGAAAATGAAGAACAAGTGGAACTCTCCTTTACAAGAACATGGGATCTGTCTCTCGAGGGAAAACTTGTGCCTTTAGAGATTGATAAAAGGTTTATAATGCTCCGGGGTTCGTCTGGGTTCTACTCTTACGCTATTTACCAACACCTGGAAGAATGGCCTGGTTTCAACCTTGGTGAAACTAGACTCGCCTTCAAACTCAGAAAAGACAAGTTCCACTATATGGCCATGGCAGACAACAGACAGAGGTACATGCCACTTCCAGATGACCGTTCTCCGCCTAGAGGTCGTGCCCTGGCATATCCAGAGGCTGTCCTGCTCGTCGACCCCATCGAACCTGAGCTCCAGGGAGAGTACTCATGCGACAACAAAGACAGCGGCGTCCATGGATGGATATCCACCGATCCGGCAGTCGGATTCTGGCTAATCTCCCCGAGCAATGAGTTCCGATCCGGTGGACCCGTTAAACAGAACCTAACTTCACACGTCGGCCCAACCACCCTAGCTGTTTTTATAAGCGCACATTACGCGGGAGAGGATCTGGTGCCGAAAATCGGAGCCGGCGAGGCGTGGAAGAAAGTTTTCGGTCCTGTGTTCATCTATCTCAACTCCACTTATGATTCCTTCGACGCACTTCAGCTCTGGGAAGATGCCAAAGCACAGGCAGTGATCGAAGTCCAGAGTTGGCCCTACAGCTTCCCTGCTTCGGATGATTTTCCAAGGTCAAACCAACGTAGTAGTATCAGTGGTAAACTATTAGTCAAGGATAGCTACGTCAATGAAGACTTGATACCAGCAGACGGTGCGTTTGTTGGCCTGGCTCCACCAGGAGACGTAGGATCATGGCAACGAGAGAGCAAGGGTTACCAATTTTGGAGTAGAGCAGATGAAAGTGGAAATTTCTCCATCGATGGCGTGAGGCCGGGCGACTACAATCTGTATGCATGGGTCCCCGGTTTTATTGGAGATTATCGTTATGATGCTCTCATCAACCTTACAGAAGGCAGTGAGGTGGACATGGGAGACATAACATACGAACCTCCGAGACAAGGAGCCACACTATGGGAAATAGGAATCCCAGATCGCTCCGCAGCAGAGTTCTACGTTCCAGATCCAAATCCCAACTACGTCAATAAGCTCTACCTTAACCATCCTGATAAGTTTCGGCAGTATGGCCTATGGGAAAGATACAGTGAATTGTTTCCCGACTCAGATTTGGTATATACCGTTGGCGTTAGTGACTACACCAAGGATTGGTTCTTTGCTCAGGTCCCCAGGAAGATGGAAAATGGGACATATAAGGGAACAACGTGGCAAGTGAAGTTCGAGCTAGACGACGTGGACAACCAAGCAACTTACAAGCTTCGGGTTGCATTGGCCTCCGTTTCTCTCGCAGAATTGCAGGTTGGAGTTAACGAGGCCAAAGCAAAGGCAGTGTTCTCGAGTGGACTTATCGGGAGGGACAATTCGATTGCAAGACACGGCATTCACGGCCTATATTGGGTTTACAACATTGACATTCCTGGAAATCGGCTGGTCACCGGCAATAACACCATCTTCTTCACCCAACCCAGATGCACAGGCCCTTTCCAAGCGATTATGTACGATTACATTCGTCTGGAAGCTCCTCCACCTCCATCCTCCGCCCATCCTTTGGAATAA

Protein sequence

MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLVWSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGSSGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRALAYPEAVLLVDPIEPELQGEYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPPSSAHPLE
Homology
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BLAST of Cucsat.G4655.T6 vs. ExPASy Swiss-Prot
Match: A5ABH4 (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 3.1e-23
Identity = 121/465 (26.02%), Postives = 186/465 (40.00%), Query Frame = 0 

Query: 206 KDSGVHGW-----ISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYAG 265
           +D+ VHG       S     G WL+  + +   GGP+  +LT  V        +S H+ G
Sbjct: 224 RDNSVHGMYADGSTSNGSTFGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNYLVSNHH-G 283

Query: 266 EDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQ-LWEDAKAQAVIEVQSWPYSFPAS- 325
           E   P I  G  + + FGP + + N    S  +LQ L  DA+  A     SW   F  S 
Sbjct: 284 EG-TPNITYG--FDRTFGPQYYHFNGGKGSTASLQELKSDAETLA---DPSWNVDFYDSI 343

Query: 326 ----DDFPRSNQRSSISGKL-LVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 385
                 +  S+QR S+ GK+ L K +     ++  DG +               S  YQ+
Sbjct: 344 AKHVVGYTPSSQRGSVQGKIKLPKGATRPIAVLTVDGQY--------FQDNSVNSSSYQY 403

Query: 386 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 445
           W+  D+SG+FS+D V+ G Y L  +  G  GD+  D  + +  G +  + + T+E    G
Sbjct: 404 WAEIDDSGHFSVDHVKEGPYRLTVYADGIFGDFVRDG-VQVKAGKKTTIQE-TWEAESAG 463

Query: 446 ATLWEIGIPDRSAAEF---YVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVY 505
             +W +G PD+S+ EF      DP         L+ P+    +G ++  S+ FPD  + Y
Sbjct: 464 TEIWRLGTPDKSSGEFRHGVARDPTHP------LHPPEYLIYWGAYDWQSD-FPDG-INY 523

Query: 506 TVGVSDYTKD-----W-FFAQVPRKMENGTYKGTTWQVKFEL--DDVDNQATYKLRVALA 565
           T+G SD   D     W  F   P            W + F L  DD+  ++   L + LA
Sbjct: 524 TIGTSDPATDLNTVHWSVFGPTPNDPRVEYDTTHDWTINFPLSEDDLAERSKATLTIQLA 583

Query: 566 SVSLAE----------------LQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYN 625
               A                 L+  +N+         G     + I R  +        
Sbjct: 584 GAKAASGNTDVYNASEPYTNLALESYINDQAEPLTLLIGFNQSSSCIVRSAVSCYQVRSR 643

Query: 626 IDIPGNRLVTGNNTIFFTQP-RCTGPFQAIMYDYI--RLEAPPPP 629
           ++ P + L  GNN +    P   T    AI+   +  RL  PP P
Sbjct: 644 MEFPADWLKVGNNVLTLHLPYNATDTETAILPATVTGRLILPPQP 661

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BLAST of Cucsat.G4655.T6 vs. ExPASy Swiss-Prot
Match: Q5AZ85 (Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2)

HSP 1 Score: 104.8 bits (260), Expect = 3.8e-21
Identity = 134/575 (23.30%), Postives = 229/575 (39.83%), Query Frame = 0 

Query: 109 EIDKRFIMLRGSSGFYSYAIYQHLEEWPGF--NLGETRLAFKLRKDKFHYMAMADNRQRY 168
           E  + + +  G +GF+ ++   +  E   F  NL E R  F+   D + ++  +D   + 
Sbjct: 118 EFQQYWFLRDGETGFHMFSRLAYYNETTPFLRNLQELRTLFRPNTDLWTHLTSSD--LQT 177

Query: 169 MPLPDDRSPPRGRALAYPEAVLLVDPIEPELQ------GEYSCDN--KDSGVHGW----- 228
            PLP D +      +      L   P +   Q       +Y+  N  +D+ VHG      
Sbjct: 178 APLPSDEAIAEQIVVQDATWRLNNTPDDAYYQQFSEYFTKYTFSNHWRDNDVHGLYADGS 237

Query: 229 ISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAW 288
            S     G WL+  + +   GGP+  +LT  V        +S H+ GE   P I  G  +
Sbjct: 238 TSDGTTYGAWLVMNTKDTYYGGPLHSDLT--VDGIIYNYIVSNHH-GEG-TPNITNG--F 297

Query: 289 KKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPYSFPAS-----DDFPRSNQRS 348
            + FGP F YL +   S    +L ++A++ A     SW   F  S       +  S+QR 
Sbjct: 298 DRTFGPQF-YLFNGGGSSSLEELRDEARSLA---SPSWNADFYDSIAKHVIGYVPSSQRG 357

Query: 349 SISGKL-LVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRES---KGYQFWSRADESGNFS 408
           S+ G + L K++     ++  DG +           +Q  S     +Q+W+  D++G FS
Sbjct: 358 SVKGTIKLPKNAKSPIAVLTVDGHY-----------FQDNSAVPSSHQYWADIDKNGRFS 417

Query: 409 IDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQGATLWEIGIPDR 468
           ID V  G Y L  +  G  GD+  D ++   +  +      T++P   G  +W +G PD+
Sbjct: 418 IDRVVAGKYRLTVYADGIFGDFTRDGIV--VKARKSTSIKETWKPESAGTEIWRLGTPDK 477

Query: 469 SAAEF---YVPDPNPNYVNKLYLNHPDKFRQY-GLWERYSELFPDSDLVYTVGVSDYTKD 528
           S+ EF      DP        +  HP ++  Y G ++  S+ FP   + Y +G SD   D
Sbjct: 478 SSGEFRHGAARDPT-------HPRHPPEYLIYWGAYDWQSD-FP-GGIDYMIGESDPATD 537

Query: 529 W------FFAQVPRKMENGTYKGTTWQVKFELD--DVDNQATYKLRVALASVSLAE---- 588
           +       F   P      +     W+++F+L    +  + T  L + LA    A     
Sbjct: 538 FNTVHWAVFGPTPDNPVAESNTTHDWRIRFDLSAKQLHARKTATLTIQLAGAKAASGNTD 597

Query: 589 ------------LQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVT 624
                       L+  +NE +       G     + I R  +         + P + L  
Sbjct: 598 VYNASEPYANLPLRSYINEQEEPLTMVIGYDQSSSCIVRSAVSCYQVREKWEFPASWLKE 657

[Close]
BLAST of Cucsat.G4655.T6 vs. ExPASy Swiss-Prot
Match: Q2U5P7 (Probable rhamnogalacturonate lyase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rglC PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.0e-14
Identity = 109/465 (23.44%), Postives = 177/465 (38.06%), Query Frame = 0 

Query: 113 RFIMLRG-SSGFYSYAIYQHLEE-WPGF-NLGETRLAFKLRKDKFHYMAMADNRQRYMPL 172
           ++  LRG  +G ++++   +  E  P   +LGE R  F+   D + + + +D      PL
Sbjct: 123 QWFFLRGEETGLHAFSRVTYFNETTPSLRSLGELRTLFRPSTDLWTHFSTSDGNYGPKPL 182

Query: 173 PDDRSPPRGRALAYPEAVLLVDPIEPELQGEYSCDN-----KDSGVHGWISTDPA----- 232
             +       A  Y   V   DP   +    ++        ++  VHG  S   +     
Sbjct: 183 GSNSGLVVQDATTYIGNV-TDDPYVSQYSDYFTKYTLAESWRNHDVHGLFSDGSSSSDGS 242

Query: 233 -VGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFG 292
             G WL+  + E   GGP+  +L   V        +S H+      P       + + +G
Sbjct: 243 TFGAWLVHNTVETYYGGPLHSDLV--VDGIVYNYLVSGHHG----APTPNLTHGFDRTWG 302

Query: 293 PVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPYSFPAS--DDFPRSNQRSSISGKLLV 352
           P F Y N          L  DA   A  E  +  Y   A    +F  S  R++  GK+ +
Sbjct: 303 PQFYYFNRGDSETTLADLRADAAKYADPEWNAEFYDSIADHIPNFTPSTGRTTFKGKVSL 362

Query: 353 KDSYVNEDLIPADGAFVGLAPPG-DVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNL 412
                      A    + L+  G D       ++  Q+W+  D+SG+FSI  V  G Y +
Sbjct: 363 PKG--------AKRPIIVLSEDGQDFQLNVFNTESLQYWAEIDKSGSFSIPRVVEGTYRI 422

Query: 413 YAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEF---YVP 472
             +     G +  D  + + +    D    T++    G  +W IGIPD+S+ E+   Y P
Sbjct: 423 TIYADEIFGWFIQDH-VKVLKSQSKDY-SFTWKEESAGKEIWRIGIPDKSSGEYLHGYAP 482

Query: 473 DPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVGVSDYTKD-----W-FFAQV 532
           D +           P++ R Y     Y   FP+  + + VG SD ++D     W FF   
Sbjct: 483 DTSKPL-------QPEQHRIYWGKYDYPADFPEG-INFHVGKSDPSQDLNYIHWAFFPSQ 542

Query: 533 PRKMENGTY--KGTTWQVKFEL--DDVDNQATYKLRVALASVSLA 548
              +    Y      W V F+L  D + N  T    V +A    A
Sbjct: 543 GNHLRTEPYYDNVNNWTVTFDLTADQLHNTNTATFTVQIAGAKTA 562

[Close]
BLAST of Cucsat.G4655.T6 vs. NCBI nr
Match: XP_004152608.2 (probable rhamnogalacturonate lyase B isoform X1 [Cucumis sativus] >KGN62737.1 hypothetical protein Csa_022438 [Cucumis sativus])

HSP 1 Score: 1315 bits (3402), Expect = 0.0
Identity = 635/641 (99.06%), Postives = 635/641 (99.06%), Query Frame = 0 

Query: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV 60
           MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV
Sbjct: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV 60

Query: 61  WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120
           WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS
Sbjct: 61  WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120

Query: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180
           SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA
Sbjct: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180

Query: 181 LAYPEAVLLVDPIEPELQGE------YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG 240
           LAYPEAVLLVDPIEPELQGE      YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG
Sbjct: 181 LAYPEAVLLVDPIEPELQGEVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG 240

Query: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300
           GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL
Sbjct: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300

Query: 301 QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG 360
           QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG
Sbjct: 301 QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG 360

Query: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420
           LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL
Sbjct: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420

Query: 421 TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480
           TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG
Sbjct: 421 TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480

Query: 481 LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT 540
           LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT
Sbjct: 481 LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT 540

Query: 541 YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL 600
           YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL
Sbjct: 541 YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL 600

Query: 601 VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPPSSAHPLE 635
           VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPPSSAHPLE
Sbjct: 601 VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPPSSAHPLE 641

[Close]
BLAST of Cucsat.G4655.T6 vs. NCBI nr
Match: XP_008444895.1 (PREDICTED: probable rhamnogalacturonate lyase B isoform X1 [Cucumis melo])

HSP 1 Score: 1274 bits (3296), Expect = 0.0
Identity = 614/643 (95.49%), Postives = 624/643 (97.05%), Query Frame = 0 

Query: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV 60
           MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRY GVDNVLEVLNDE+NRGYWDLV
Sbjct: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNVLEVLNDETNRGYWDLV 60

Query: 61  WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120
           WSSSSS+TGLFDVIKATGF+VIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS
Sbjct: 61  WSSSSSSTGLFDVIKATGFKVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120

Query: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180
           SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA
Sbjct: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180

Query: 181 LAYPEAVLLVDPIEPELQGE------YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG 240
           LAYPEAV L+DPIEPELQGE      YSCDNKDSGVHGWISTDP VGFWLISPSNEFRSG
Sbjct: 181 LAYPEAVRLIDPIEPELQGEVDDKYQYSCDNKDSGVHGWISTDPTVGFWLISPSNEFRSG 240

Query: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300
           GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL
Sbjct: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300

Query: 301 QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG 360
           QLWEDAKAQA +EVQSWPYSFPASDDFPRSNQR SISGKLLVKDSYV+EDLIPA GAFVG
Sbjct: 301 QLWEDAKAQAAVEVQSWPYSFPASDDFPRSNQRGSISGKLLVKDSYVDEDLIPAGGAFVG 360

Query: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420
           LAPPGDVGSWQRESKGYQFWSRADESGNF IDGVRPGDYNLYAWVPGFIGDYRYDALINL
Sbjct: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFFIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420

Query: 421 TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480
            EGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNY+NKLYLNHPDKFRQYG
Sbjct: 421 KEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYINKLYLNHPDKFRQYG 480

Query: 481 LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT 540
           LWERYSELFPDSDLVY VGVSDYTKDWFFAQVPRK+ENGTYKGTTWQVKFELD+VDNQAT
Sbjct: 481 LWERYSELFPDSDLVYNVGVSDYTKDWFFAQVPRKIENGTYKGTTWQVKFELDNVDNQAT 540

Query: 541 YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL 600
           YKLRVALAS SLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYW+YNIDIPGNRL
Sbjct: 541 YKLRVALASASLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWLYNIDIPGNRL 600

Query: 601 VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPP--SSAHPLE 635
           VTGNNTIFFTQPR TGPFQAIMYDYIRLEAPPPP  SSAHPLE
Sbjct: 601 VTGNNTIFFTQPRSTGPFQAIMYDYIRLEAPPPPPPSSAHPLE 643

[Close]
BLAST of Cucsat.G4655.T6 vs. NCBI nr
Match: KAA0065109.1 (putative rhamnogalacturonate lyase B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1269 bits (3285), Expect = 0.0
Identity = 613/643 (95.33%), Postives = 622/643 (96.73%), Query Frame = 0 

Query: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV 60
           MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRY GVDNVLEVLNDE+NRGYWDLV
Sbjct: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNVLEVLNDETNRGYWDLV 60

Query: 61  WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120
           WSSSSS+TGLFDVIKATGF+VIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS
Sbjct: 61  WSSSSSSTGLFDVIKATGFKVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120

Query: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180
           SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA
Sbjct: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180

Query: 181 LAYPEAVLLVDPIEPELQGE------YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG 240
           LAYPEAV L+DPIEPELQGE      YSCDNKDSGVHGWISTDP VGFWLISPSNEFRSG
Sbjct: 181 LAYPEAVRLIDPIEPELQGEVDDKYQYSCDNKDSGVHGWISTDPTVGFWLISPSNEFRSG 240

Query: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300
           GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL
Sbjct: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300

Query: 301 QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG 360
           QLWEDAKAQA IEVQSWPYSFPASDDFPRSNQR SISGKLLVKDSYV+EDLIPA GAFVG
Sbjct: 301 QLWEDAKAQAAIEVQSWPYSFPASDDFPRSNQRGSISGKLLVKDSYVDEDLIPAGGAFVG 360

Query: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420
           LAPPGDVGSWQRESKGYQFWSRADESGNF IDG RPGDYNLYAWVPGFIGDYRYDALINL
Sbjct: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFFIDGARPGDYNLYAWVPGFIGDYRYDALINL 420

Query: 421 TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480
            EGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNY+NKLYLNHPDKFRQYG
Sbjct: 421 KEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYINKLYLNHPDKFRQYG 480

Query: 481 LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT 540
           LWERYSELFPDSDLVY VGVSDYTKDWFFAQVPR +ENGTYKGTTWQVKFELD+VDNQAT
Sbjct: 481 LWERYSELFPDSDLVYNVGVSDYTKDWFFAQVPRCIENGTYKGTTWQVKFELDNVDNQAT 540

Query: 541 YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL 600
           YKLRVALAS SLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYW+YNIDIPGNRL
Sbjct: 541 YKLRVALASASLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWLYNIDIPGNRL 600

Query: 601 VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPP--SSAHPLE 635
           VTGNNTIFFTQPR TGPFQAIMYDYIRLEAPPPP  SSAHPLE
Sbjct: 601 VTGNNTIFFTQPRSTGPFQAIMYDYIRLEAPPPPPPSSAHPLE 643

[Close]
BLAST of Cucsat.G4655.T6 vs. NCBI nr
Match: XP_038886212.1 (probable rhamnogalacturonate lyase B isoform X1 [Benincasa hispida])

HSP 1 Score: 1230 bits (3183), Expect = 0.0
Identity = 589/642 (91.74%), Postives = 615/642 (95.79%), Query Frame = 0 

Query: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV 60
           MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRY GVDNVLEVLNDESNRGYWDLV
Sbjct: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNVLEVLNDESNRGYWDLV 60

Query: 61  WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120
           W+SS+STTGLFDVIK+TGFEVIVENE+QVELSFTR+WDLSLEGKLVPLEIDKRFIMLRGS
Sbjct: 61  WNSSASTTGLFDVIKSTGFEVIVENEDQVELSFTRSWDLSLEGKLVPLEIDKRFIMLRGS 120

Query: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180
           SGFYSYAIYQHL+EWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA
Sbjct: 121 SGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180

Query: 181 LAYPEAVLLVDPIEPELQGE------YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG 240
           LAYPEAVLLVDPIEPEL GE      YSCDNKDSGVHGWISTDPAVGFWLI+PSNEFRSG
Sbjct: 181 LAYPEAVLLVDPIEPELLGEVDDKYQYSCDNKDSGVHGWISTDPAVGFWLITPSNEFRSG 240

Query: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300
           GPVKQNLTSHVGPTTLAVFISAHY GEDLVPKIGA E WKKVFGPVFIYLNSTYDS DAL
Sbjct: 241 GPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGADEEWKKVFGPVFIYLNSTYDSTDAL 300

Query: 301 QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG 360
           QLWEDAKAQA IEVQSWPYSFPASDDFP+ NQR SISG+LLV+DSYV+ DLIPA+GAFVG
Sbjct: 301 QLWEDAKAQATIEVQSWPYSFPASDDFPKLNQRGSISGRLLVRDSYVSGDLIPANGAFVG 360

Query: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420
           LAPPGDVGSWQRESKGYQFWSRADESG FSIDGVRP DYNL+AWVPGFIGDYRYDALI +
Sbjct: 361 LAPPGDVGSWQRESKGYQFWSRADESGKFSIDGVRPDDYNLFAWVPGFIGDYRYDALIGI 420

Query: 421 TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480
           TEG E+DM DITYEPPRQGAT+WEIGIPDRSAAEFYVP+PNPNYVNKLYLNHPDKFRQYG
Sbjct: 421 TEGIEIDMDDITYEPPRQGATIWEIGIPDRSAAEFYVPNPNPNYVNKLYLNHPDKFRQYG 480

Query: 481 LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT 540
           LWERYSEL+PD+DLVYTVG+SDYTKDWFFAQV RK ENGTYKGTTWQVKFELD+VDN+AT
Sbjct: 481 LWERYSELYPDADLVYTVGISDYTKDWFFAQVTRKTENGTYKGTTWQVKFELDNVDNKAT 540

Query: 541 YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL 600
           YKLR+ALAS SLAELQVGVNEAKAK VFSSGLIGRDNSIARHGIHGL+WVYN+DIPGNRL
Sbjct: 541 YKLRIALASASLAELQVGVNEAKAKPVFSSGLIGRDNSIARHGIHGLHWVYNVDIPGNRL 600

Query: 601 VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPP-SSAHPLE 635
           VTG+NTIFFTQPR TGPFQAIMYDYIRLEAPPPP SSAHPLE
Sbjct: 601 VTGDNTIFFTQPRSTGPFQAIMYDYIRLEAPPPPPSSAHPLE 642

[Close]
BLAST of Cucsat.G4655.T6 vs. NCBI nr
Match: XP_023537007.1 (probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537008.1 probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537009.1 probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537010.1 probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1187 bits (3071), Expect = 0.0
Identity = 565/640 (88.28%), Postives = 600/640 (93.75%), Query Frame = 0 

Query: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV 60
           MPPLGVQL I E QVTMDNGIVQVTLSNPDGIVTGIRY GVDN+LEVLNDESNRGYWDLV
Sbjct: 1   MPPLGVQLSIAEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLV 60

Query: 61  WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120
           W+ S+S TG FDVIKAT FEVIVENE+QVELSFTR WDLSLEGKLVPLEIDKRFIMLRGS
Sbjct: 61  WNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLEIDKRFIMLRGS 120

Query: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180
           SGFYSYAIYQHL+EWPGFNLGETRLAFKLRKD+FHYMAMADNRQR MPLPDDRSPPRGRA
Sbjct: 121 SGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDRFHYMAMADNRQRSMPLPDDRSPPRGRA 180

Query: 181 LAYPEAVLLVDPIEPELQGE------YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG 240
           LAYPEAVLLVDPIE ELQGE      YSCDNKDSGVHGWISTDP +GFWLI+PSNE RSG
Sbjct: 181 LAYPEAVLLVDPIESELQGEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSG 240

Query: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300
           GPVKQNLTSHVGPTTLAVFISAHY GEDLVPKIGAGEAWKKVFGPVFIYLNSTYDS DAL
Sbjct: 241 GPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAL 300

Query: 301 QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG 360
           QLWED K+QA IEVQSWPYSFPAS+DFP+SNQR +ISG+LLVKDSYV+ED IPA+GAFVG
Sbjct: 301 QLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVG 360

Query: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420
           LAPPGDVGSWQRESKGYQFWSRADE G F ID VRPGDYNLYAWVPGFIGDYRYD++IN+
Sbjct: 361 LAPPGDVGSWQRESKGYQFWSRADEDGYFLIDHVRPGDYNLYAWVPGFIGDYRYDSIINI 420

Query: 421 TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480
           TEGS++D+ +I YEPPR GATLWEIG+PDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG
Sbjct: 421 TEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480

Query: 481 LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT 540
           LWERYSEL+PD+DLVYTVGVSDYTKDWFFAQV RK ENGT+KGTTWQVKFELD+VD+QAT
Sbjct: 481 LWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQAT 540

Query: 541 YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL 600
           Y+LRVALAS SLAELQVGVN+ KAK VFSSGLIGRDNSIARHGIHGLYW+YN++IPGNRL
Sbjct: 541 YQLRVALASASLAELQVGVNDPKAKPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRL 600

Query: 601 VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPPSSAHPL 634
           V+G+NTIFFTQPR T PFQAIMYDYIRLEAPPPPSSAH L
Sbjct: 601 VSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGL 640

[Close]
BLAST of Cucsat.G4655.T6 vs. ExPASy TrEMBL
Match: A0A0A0LPL0 (Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_2G370370 PE=3 SV=1)

HSP 1 Score: 1315 bits (3402), Expect = 0.0
Identity = 635/641 (99.06%), Postives = 635/641 (99.06%), Query Frame = 0 

Query: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV 60
           MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV
Sbjct: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV 60

Query: 61  WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120
           WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS
Sbjct: 61  WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120

Query: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180
           SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA
Sbjct: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180

Query: 181 LAYPEAVLLVDPIEPELQGE------YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG 240
           LAYPEAVLLVDPIEPELQGE      YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG
Sbjct: 181 LAYPEAVLLVDPIEPELQGEVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG 240

Query: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300
           GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL
Sbjct: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300

Query: 301 QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG 360
           QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG
Sbjct: 301 QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG 360

Query: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420
           LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL
Sbjct: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420

Query: 421 TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480
           TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG
Sbjct: 421 TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480

Query: 481 LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT 540
           LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT
Sbjct: 481 LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT 540

Query: 541 YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL 600
           YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL
Sbjct: 541 YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL 600

Query: 601 VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPPSSAHPLE 635
           VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPPSSAHPLE
Sbjct: 601 VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPPSSAHPLE 641

[Close]
BLAST of Cucsat.G4655.T6 vs. ExPASy TrEMBL
Match: A0A1S3BCA7 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103488110 PE=3 SV=1)

HSP 1 Score: 1274 bits (3296), Expect = 0.0
Identity = 614/643 (95.49%), Postives = 624/643 (97.05%), Query Frame = 0 

Query: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV 60
           MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRY GVDNVLEVLNDE+NRGYWDLV
Sbjct: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNVLEVLNDETNRGYWDLV 60

Query: 61  WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120
           WSSSSS+TGLFDVIKATGF+VIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS
Sbjct: 61  WSSSSSSTGLFDVIKATGFKVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120

Query: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180
           SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA
Sbjct: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180

Query: 181 LAYPEAVLLVDPIEPELQGE------YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG 240
           LAYPEAV L+DPIEPELQGE      YSCDNKDSGVHGWISTDP VGFWLISPSNEFRSG
Sbjct: 181 LAYPEAVRLIDPIEPELQGEVDDKYQYSCDNKDSGVHGWISTDPTVGFWLISPSNEFRSG 240

Query: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300
           GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL
Sbjct: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300

Query: 301 QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG 360
           QLWEDAKAQA +EVQSWPYSFPASDDFPRSNQR SISGKLLVKDSYV+EDLIPA GAFVG
Sbjct: 301 QLWEDAKAQAAVEVQSWPYSFPASDDFPRSNQRGSISGKLLVKDSYVDEDLIPAGGAFVG 360

Query: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420
           LAPPGDVGSWQRESKGYQFWSRADESGNF IDGVRPGDYNLYAWVPGFIGDYRYDALINL
Sbjct: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFFIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420

Query: 421 TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480
            EGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNY+NKLYLNHPDKFRQYG
Sbjct: 421 KEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYINKLYLNHPDKFRQYG 480

Query: 481 LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT 540
           LWERYSELFPDSDLVY VGVSDYTKDWFFAQVPRK+ENGTYKGTTWQVKFELD+VDNQAT
Sbjct: 481 LWERYSELFPDSDLVYNVGVSDYTKDWFFAQVPRKIENGTYKGTTWQVKFELDNVDNQAT 540

Query: 541 YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL 600
           YKLRVALAS SLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYW+YNIDIPGNRL
Sbjct: 541 YKLRVALASASLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWLYNIDIPGNRL 600

Query: 601 VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPP--SSAHPLE 635
           VTGNNTIFFTQPR TGPFQAIMYDYIRLEAPPPP  SSAHPLE
Sbjct: 601 VTGNNTIFFTQPRSTGPFQAIMYDYIRLEAPPPPPPSSAHPLE 643

[Close]
BLAST of Cucsat.G4655.T6 vs. ExPASy TrEMBL
Match: A0A5A7VI09 (Rhamnogalacturonan endolyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004440 PE=3 SV=1)

HSP 1 Score: 1269 bits (3285), Expect = 0.0
Identity = 613/643 (95.33%), Postives = 622/643 (96.73%), Query Frame = 0 

Query: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV 60
           MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRY GVDNVLEVLNDE+NRGYWDLV
Sbjct: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNVLEVLNDETNRGYWDLV 60

Query: 61  WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120
           WSSSSS+TGLFDVIKATGF+VIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS
Sbjct: 61  WSSSSSSTGLFDVIKATGFKVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120

Query: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180
           SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA
Sbjct: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180

Query: 181 LAYPEAVLLVDPIEPELQGE------YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG 240
           LAYPEAV L+DPIEPELQGE      YSCDNKDSGVHGWISTDP VGFWLISPSNEFRSG
Sbjct: 181 LAYPEAVRLIDPIEPELQGEVDDKYQYSCDNKDSGVHGWISTDPTVGFWLISPSNEFRSG 240

Query: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300
           GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL
Sbjct: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300

Query: 301 QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG 360
           QLWEDAKAQA IEVQSWPYSFPASDDFPRSNQR SISGKLLVKDSYV+EDLIPA GAFVG
Sbjct: 301 QLWEDAKAQAAIEVQSWPYSFPASDDFPRSNQRGSISGKLLVKDSYVDEDLIPAGGAFVG 360

Query: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420
           LAPPGDVGSWQRESKGYQFWSRADESGNF IDG RPGDYNLYAWVPGFIGDYRYDALINL
Sbjct: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFFIDGARPGDYNLYAWVPGFIGDYRYDALINL 420

Query: 421 TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480
            EGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNY+NKLYLNHPDKFRQYG
Sbjct: 421 KEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYINKLYLNHPDKFRQYG 480

Query: 481 LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT 540
           LWERYSELFPDSDLVY VGVSDYTKDWFFAQVPR +ENGTYKGTTWQVKFELD+VDNQAT
Sbjct: 481 LWERYSELFPDSDLVYNVGVSDYTKDWFFAQVPRCIENGTYKGTTWQVKFELDNVDNQAT 540

Query: 541 YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL 600
           YKLRVALAS SLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYW+YNIDIPGNRL
Sbjct: 541 YKLRVALASASLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWLYNIDIPGNRL 600

Query: 601 VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPP--SSAHPLE 635
           VTGNNTIFFTQPR TGPFQAIMYDYIRLEAPPPP  SSAHPLE
Sbjct: 601 VTGNNTIFFTQPRSTGPFQAIMYDYIRLEAPPPPPPSSAHPLE 643

[Close]
BLAST of Cucsat.G4655.T6 vs. ExPASy TrEMBL
Match: A0A6J1KMB3 (Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111495919 PE=3 SV=1)

HSP 1 Score: 1179 bits (3050), Expect = 0.0
Identity = 560/640 (87.50%), Postives = 597/640 (93.28%), Query Frame = 0 

Query: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV 60
           MPPLGVQL I+E QVTMDNGIVQVTLSNPDGIVTGIRY GVDN+LEVLNDESNRGYWDLV
Sbjct: 1   MPPLGVQLSISEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLV 60

Query: 61  WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120
           W+ S+S TG FDVIKAT FEVIVENE+QVELSFTR WDLSLEGKLVPLEIDKRFIMLRGS
Sbjct: 61  WNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLEIDKRFIMLRGS 120

Query: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180
           SGFYSYAIYQHL+EWPGFNLGETRLAFKLRKDKFHYMAMADNRQR MPLPDDRSPPRGRA
Sbjct: 121 SGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRA 180

Query: 181 LAYPEAVLLVDPIEPELQGE------YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG 240
           LAYPEAVLLVDPIE ELQGE      YSCDNKDSGVHGWISTDP +GFWLI+PSNE RSG
Sbjct: 181 LAYPEAVLLVDPIESELQGEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSG 240

Query: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300
           GPVKQNLTSHVGPT+LAVFISAHY GEDLVPKIGAGEAWKKVFGPVFIYLNSTYD  DA 
Sbjct: 241 GPVKQNLTSHVGPTSLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDFSDAF 300

Query: 301 QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG 360
           QLWED K+QA IEVQSWPYSFPAS+DFP+SNQR +ISG+LLVKDSYV+ED IPA+GAFVG
Sbjct: 301 QLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVG 360

Query: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420
           LAPPGDVGSWQRESKGYQFWSRADE G F ID VRPGDYNLYAWVPGFIGDYRYD++IN+
Sbjct: 361 LAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHVRPGDYNLYAWVPGFIGDYRYDSIINI 420

Query: 421 TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480
            EGS++D+ +I YEPPR GATLWEIG+PDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG
Sbjct: 421 IEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480

Query: 481 LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT 540
           LWERYSEL+PD+DLVYTVGVSDYTKDWFFAQV RK ENGT+KGTTWQVKFELD+VD+QAT
Sbjct: 481 LWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQAT 540

Query: 541 YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL 600
           YKLRVALAS S+ ELQVGVN+ KAK +FSSGLIGRDNSIARHGIHGLYW+YN++IPGNRL
Sbjct: 541 YKLRVALASASIVELQVGVNDPKAKPLFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRL 600

Query: 601 VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPPSSAHPL 634
           V+G+NTIFFTQPR T PFQAIMYDYIRLEAPPPPSSAH L
Sbjct: 601 VSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGL 640

[Close]
BLAST of Cucsat.G4655.T6 vs. ExPASy TrEMBL
Match: A0A6J1GGG5 (Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111454003 PE=3 SV=1)

HSP 1 Score: 1175 bits (3039), Expect = 0.0
Identity = 559/640 (87.34%), Postives = 596/640 (93.12%), Query Frame = 0 

Query: 1   MPPLGVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLV 60
           MPPLGVQL I E QVTMDNGIVQVTLSNPDGIVTGIRY GVDN+LEVLNDESNRGYWDLV
Sbjct: 1   MPPLGVQLSIAEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLV 60

Query: 61  WSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGS 120
           W+ S+S TG FDVIKAT FEVIVENE+QVELSFTR WDLSLEGKLVPL IDKRFIMLRGS
Sbjct: 61  WNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLLIDKRFIMLRGS 120

Query: 121 SGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRA 180
           SGFYSYAIYQHL+EWPGFNLGETRLAFKLRKDKFHYMAMADNRQR MPLPDDRSPPRGRA
Sbjct: 121 SGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRA 180

Query: 181 LAYPEAVLLVDPIEPELQGE------YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSG 240
           LAYPEAVLLVDPIE EL+GE      YSCDNKDSGVHGWISTDP +GFWLI+PSNE RSG
Sbjct: 181 LAYPEAVLLVDPIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSG 240

Query: 241 GPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDAL 300
           GPVKQNLTSHVGPTTLAVFISAHY GEDLVPKIGAGEAWKKVFGPVFIYLNSTYDS DAL
Sbjct: 241 GPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAL 300

Query: 301 QLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVG 360
           QLWED K+QA IEVQSWPYSFPAS+DFP+SNQR +ISG+LLVKDSYV+ED IPA+GAFVG
Sbjct: 301 QLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVG 360

Query: 361 LAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINL 420
           LAPPGDVGSWQRESKGYQFWSRADE G F ID +RPGDYNLYAWVPGFIGDYRYD++I++
Sbjct: 361 LAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFIGDYRYDSIIDI 420

Query: 421 TEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480
            EGS++D+ +I YEPPR GATLWEIG+PDRSA EFYVPDPNPNYVNKLYLNH DKFRQYG
Sbjct: 421 LEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLYLNHSDKFRQYG 480

Query: 481 LWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT 540
           LWERYSEL+PD+DLVYTVGVSDYTKDWFFAQV RK ENGT+KGTTWQVKFELD+VD+QAT
Sbjct: 481 LWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQAT 540

Query: 541 YKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRL 600
           YKLRVALAS S+AELQVGVN+ KAK VFSSGLIGRDNSIARHGIHGLYW+YN++IPGNRL
Sbjct: 541 YKLRVALASASIAELQVGVNDPKAKPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRL 600

Query: 601 VTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPPSSAHPL 634
           V+G+NTIFFTQPR T PFQAIMYDYIRLEAPPPPSSAH L
Sbjct: 601 VSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGL 640

[Close]
BLAST of Cucsat.G4655.T6 vs. TAIR 10
Match: AT1G09890.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 931.0 bits (2405), Expect = 5.0e-271
Identity = 425/617 (68.88%), Postives = 506/617 (82.01%), Query Frame = 0 

Query: 17  MDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLVWSSSSSTTGLFDVIKA 76
           MDNGI +VTLS PDGIVTGI Y G+DN+LEVLN+E NRGYWDLVW   S T G FDVIK 
Sbjct: 1   MDNGIARVTLSKPDGIVTGIEYNGIDNLLEVLNEEVNRGYWDLVW-GGSGTAGGFDVIKG 60

Query: 77  TGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGSSGFYSYAIYQHLEEWP 136
           + FEVIV+NEEQ+ELSFTR WD S EGK VPL IDKRF+ML GSSGFY+YAIY+HL+EWP
Sbjct: 61  SNFEVIVKNEEQIELSFTRKWDPSQEGKAVPLNIDKRFVMLSGSSGFYTYAIYEHLKEWP 120

Query: 137 GFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRALAYPEAVLLVDPIEPE 196
            F+L ETR+AFKLRK+KFHYMA+ D+RQR+MPLPDDR P RG+ALAYPEAVLLV+P+E +
Sbjct: 121 AFSLAETRIAFKLRKEKFHYMAVTDDRQRFMPLPDDRLPDRGQALAYPEAVLLVNPLESQ 180

Query: 197 LQGE------YSCDNKDSGVHGWISTD-PAVGFWLISPSNEFRSGGPVKQNLTSHVGPTT 256
            +GE      YSC+NKD  VHGWI T+ P+VGFWLI+PS+E+R+GGP KQNLTSHVGPT 
Sbjct: 181 FKGEVDDKYQYSCENKDITVHGWICTEQPSVGFWLITPSHEYRTGGPQKQNLTSHVGPTA 240

Query: 257 LAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLN-STYDSFDALQLWEDAKAQAVIEV 316
           LAVFISAHY GEDLVPK   GEAWKKVFGPVF+YLN ST D  D L LW+DAK+Q  +E 
Sbjct: 241 LAVFISAHYTGEDLVPKFSEGEAWKKVFGPVFVYLNSSTDDDNDPLWLWQDAKSQMNVEA 300

Query: 317 QSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRES 376
           +SWPYSFPASDD+ ++ QR ++ G+LLV+D YV++D I A+  +VGLA PG  GSWQRE 
Sbjct: 301 ESWPYSFPASDDYVKTEQRGNVVGRLLVQDRYVDKDFIAANRGYVGLAVPGAAGSWQREC 360

Query: 377 KGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYE 436
           K YQFW+R DE G F I G+RPG YNLYAW+PGFIGDY+YD +I +T G  + + D+ Y+
Sbjct: 361 KEYQFWTRTDEEGFFYISGIRPGQYNLYAWIPGFIGDYKYDDVITITSGCYIYVEDLVYQ 420

Query: 437 PPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDL 496
           PPR GATLWEIG PDRSAAEFYVPDPNP Y+N LY NHPD+FRQYGLWERY+EL+PD DL
Sbjct: 421 PPRNGATLWEIGFPDRSAAEFYVPDPNPKYINNLYQNHPDRFRQYGLWERYAELYPDKDL 480

Query: 497 VYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAE 556
           VY VG SDY KDWF+AQV RK +N TY+GTTWQ+KFEL ++D   +Y LRVA+AS + +E
Sbjct: 481 VYVVGSSDYRKDWFYAQVTRKKDNKTYQGTTWQIKFELKNIDKNHSYTLRVAIASATFSE 540

Query: 557 LQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRC 616
           LQ+ VN A A  +F+SGLIGRDNSIARHGIHGLYW++N+++ G++L+ G NT+F TQPR 
Sbjct: 541 LQIRVNNANASPMFTSGLIGRDNSIARHGIHGLYWLFNVEVAGSKLLEGENTLFLTQPRS 600

Query: 617 TGPFQAIMYDYIRLEAP 626
           T PFQ IMYDYIR EAP
Sbjct: 601 TSPFQGIMYDYIRFEAP 616

[Close]
BLAST of Cucsat.G4655.T6 vs. TAIR 10
Match: AT1G09910.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 893.6 bits (2308), Expect = 8.9e-260
Identity = 410/629 (65.18%), Postives = 500/629 (79.49%), Query Frame = 0 

Query: 5   GVQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLVWSSS 64
           GV L + +R V MDNGI+QVTLS P GI+TGI Y G+DNVLEV N E+NRGYWDL W+  
Sbjct: 48  GVHLHVHDRYVVMDNGILQVTLSKPGGIITGIEYNGIDNVLEVRNKETNRGYWDLHWNEP 107

Query: 65  SSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGSSGFY 124
               G+FDVI    F VIVE EEQVE+SF RTWD SLEGK +PL IDKRFIMLRGSSG Y
Sbjct: 108 GG-KGIFDVISGVTFRVIVETEEQVEISFLRTWDPSLEGKYIPLNIDKRFIMLRGSSGVY 167

Query: 125 SYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRALAYP 184
           SY IY+HL++WPGF LGETR+AFKLRKDKFHYMA+AD+R+R MP PDD    R + L Y 
Sbjct: 168 SYGIYEHLKDWPGFELGETRIAFKLRKDKFHYMAVADDRKRIMPFPDDLCKGRCQTLDYQ 227

Query: 185 EAVLLVDPIEPELQGE------YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVK 244
           EA LL  P +P LQGE      YSC+NKD  VHGWIS DP VGFW I+PSNEFRSGGP+K
Sbjct: 228 EASLLTAPCDPRLQGEVDDKYQYSCENKDLRVHGWISFDPPVGFWQITPSNEFRSGGPLK 287

Query: 245 QNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWE 304
           QNLTSHVGPTTLAVF S HYAG+ ++P+   GE WKKV+GPVFIYLNST +  D L LW+
Sbjct: 288 QNLTSHVGPTTLAVFHSTHYAGKTMMPRFEHGEPWKKVYGPVFIYLNSTANGDDPLCLWD 347

Query: 305 DAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPP 364
           DAK + + EV+ WPYSF ASDD+P+S +R +  G+LL++D ++N DLI A GA+VGLAPP
Sbjct: 348 DAKIKMMAEVERWPYSFVASDDYPKSEERGTARGRLLIRDRFINNDLISARGAYVGLAPP 407

Query: 365 GDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGS 424
           GD GSWQ E KGYQFW+ ADE+G FSI  VRPG+YNLYAWVP FIGDY    ++ +T G 
Sbjct: 408 GDSGSWQIECKGYQFWAIADEAGYFSIGNVRPGEYNLYAWVPSFIGDYHNGTIVRVTSGC 467

Query: 425 EVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWER 484
            ++MGDI YEPPR G TLWEIGIPDR A+EF++PDP+P  VN++ ++H D+FRQYGLW++
Sbjct: 468 MIEMGDIVYEPPRDGPTLWEIGIPDRKASEFFIPDPDPTLVNRVLVHHQDRFRQYGLWKK 527

Query: 485 YSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLR 544
           Y++++P+ DLVYTVGVSDY +DWFFA VPRK +   ++GTTWQ+ F L+++D +A YKLR
Sbjct: 528 YTDMYPNDDLVYTVGVSDYRRDWFFAHVPRK-KGDVHEGTTWQIIFNLENIDQKANYKLR 587

Query: 545 VALASVSLAELQVGVNEAKA-KAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTG 604
           VA+AS +LAELQ+ +N+A+A + +F++GLIGRDNSIARHGIHG+Y +Y ++IPGNRLV G
Sbjct: 588 VAIASATLAELQIRINDAEAIRPLFTTGLIGRDNSIARHGIHGVYMLYAVNIPGNRLVQG 647

Query: 605 NNTIFFTQPRCTGPFQAIMYDYIRLEAPP 627
           +NTIF  QPRC GPFQ IMYDYIRLE PP
Sbjct: 648 DNTIFLKQPRCNGPFQGIMYDYIRLEGPP 674

[Close]
BLAST of Cucsat.G4655.T6 vs. TAIR 10
Match: AT4G24430.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 856.7 bits (2212), Expect = 1.2e-248
Identity = 399/640 (62.34%), Postives = 499/640 (77.97%), Query Frame = 0 

Query: 6   VQLFITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDLVWSSSS 65
           VQL + E  V M NG V+VT+S PDG VTGI Y GVDN+LE  N++ NRGYWDLVWS   
Sbjct: 7   VQLDVQESHVVMGNGKVKVTISKPDGFVTGISYQGVDNLLETHNEDFNRGYWDLVWSDEG 66

Query: 66  S--TTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGSSGF 125
           +  TTG  + IK T FEV+VENEE VE+SF+R WD SL+  + P+ +DKRFIM +  +GF
Sbjct: 67  TPGTTGKSERIKGTSFEVVVENEELVEISFSRKWDSSLQDSIAPINVDKRFIMRKDVTGF 126

Query: 126 YSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRALAY 185
           YSYAI++HL EWP FNL +TR+ +KLRKDKF YMA+ADNRQR MPLP+DR   RGR LAY
Sbjct: 127 YSYAIFEHLAEWPAFNLPQTRIVYKLRKDKFKYMAIADNRQRKMPLPEDRLGKRGRPLAY 186

Query: 186 PEAVLLVDPIEPELQG------EYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPV 245
           PEAVLLV P+E E +G      EYS +NKD  VHGWIS +  +G W I PSNEFRSGG  
Sbjct: 187 PEAVLLVHPVEDEFKGEVDDKYEYSSENKDLKVHGWISHNLDLGCWQIIPSNEFRSGGLS 246

Query: 246 KQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYD-SFDALQL 305
           KQNLTSHVGP +LA+F+SAHYAGED+V K+ AG++WKKVFGPVF YLN   D + D L L
Sbjct: 247 KQNLTSHVGPISLAMFLSAHYAGEDMVMKVKAGDSWKKVFGPVFTYLNCLPDKTSDPLSL 306

Query: 306 WEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLA 365
           W+DAK Q + EVQSWPY FPAS+DFP S++R  ISG+LLV D ++++D +PA+GAFVGLA
Sbjct: 307 WQDAKNQMLTEVQSWPYDFPASEDFPVSDKRGCISGRLLVCDKFLSDDFLPANGAFVGLA 366

Query: 366 PPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTE 425
           PPG+VGSWQ ESKGYQFW+ AD  G F+I+ +R G+YNL  +V G+IGDY+Y+ LIN+T 
Sbjct: 367 PPGEVGSWQLESKGYQFWTEADSDGYFAINDIREGEYNLNGYVTGWIGDYQYEQLINITA 426

Query: 426 GSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLW 485
           G ++D+G+I YEPPR G T+WEIGIPDRSAAEF+VPDPNP Y+NKLY+ HPD+FRQYGLW
Sbjct: 427 GCDIDVGNIVYEPPRDGPTVWEIGIPDRSAAEFFVPDPNPKYINKLYIGHPDRFRQYGLW 486

Query: 486 ERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYK 545
           ERY+EL+P  DLV+T+GVSDY KDWFFA V RKM + TY+ TTWQ+KF+L++V    TYK
Sbjct: 487 ERYTELYPKEDLVFTIGVSDYKKDWFFAHVTRKMGDDTYQKTTWQIKFKLENVQKSCTYK 546

Query: 546 LRVALASVSLAELQVGVNE---AKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNR 605
           +R+ALA+ ++AELQV +N+    K   +F++G+IG DN+IARHGIHG+Y +YN+D+P  +
Sbjct: 547 IRIALATANVAELQVRMNDDDTEKTTPIFTTGVIGHDNAIARHGIHGIYRLYNVDVPSEK 606

Query: 606 LVTGNNTIFFTQP-RCTGPFQAIMYDYIRLEAPPPPSSAH 633
           LV G+NT+F TQ    TG F  +MYDYIRLE PP  S +H
Sbjct: 607 LVEGDNTLFLTQTMTTTGAFNGLMYDYIRLEGPPLDSYSH 646

[Close]
BLAST of Cucsat.G4655.T6 vs. TAIR 10
Match: AT1G09880.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 828.9 bits (2140), Expect = 2.7e-240
Identity = 386/623 (61.96%), Postives = 479/623 (76.89%), Query Frame = 0 

Query: 17  MDNGIVQVTLSNPDGIVTGIRYGGVDNVLE-VLNDESNRGYWDLVWS----SSSSTTGLF 76
           M+N  +Q+TLSNP+G VTGI+Y G+DNVL    N E +RGYWD+VW+     +  T G  
Sbjct: 1   MENRFLQLTLSNPEGFVTGIQYNGIDNVLAYYTNKEYDRGYWDVVWNFPGKKAKKTKGTL 60

Query: 77  DVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRGSSGFYSYAIYQH 136
           D I+AT  EVI +N+E++ELSFTRTW+ S     VP+ IDKRF+ML+ SSGFYSYAI++ 
Sbjct: 61  DRIEATKMEVITQNDEKIELSFTRTWNTS-STTAVPVNIDKRFVMLQNSSGFYSYAIFER 120

Query: 137 LEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGRALAYPEAVLLVD 196
           L+ WP   L   RL FKL K KFHYMA++D+RQRYMP+PDDR PPRG+ LAYPEAV L+D
Sbjct: 121 LQGWPAVELDNMRLVFKLNKKKFHYMAISDDRQRYMPVPDDRVPPRGQPLAYPEAVQLLD 180

Query: 197 PIEPELQG------EYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHV 256
           PIEPE +G      EYS ++KD  VHGWIST+ +VGFW I+PSNEFRS GP+KQ L SHV
Sbjct: 181 PIEPEFKGEVDDKYEYSMESKDIKVHGWISTNDSVGFWQITPSNEFRSAGPLKQFLGSHV 240

Query: 257 GPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAV 316
           GPT LAVF S HY G DL+     GEAWKKVFGPVFIYLNS     D L LW +AK Q  
Sbjct: 241 GPTNLAVFHSTHYVGADLIMSFKNGEAWKKVFGPVFIYLNSFPKGVDPLLLWHEAKNQTK 300

Query: 317 IEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQ 376
           IE + WPY+F ASDDFP S+QR S+SG+LLV+D +++ + IPA+G++VGLA PGDVGSWQ
Sbjct: 301 IEEEKWPYNFTASDDFPASDQRGSVSGRLLVRDRFISSEDIPANGSYVGLAAPGDVGSWQ 360

Query: 377 RESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDI 436
           RE KGYQFWS+ADE+G+FSI+ VR G YNLYA+ PGFIGDY  D + +++ GS++ +GD+
Sbjct: 361 RECKGYQFWSKADENGSFSINNVRSGRYNLYAFAPGFIGDYHNDTVFDISPGSKISLGDL 420

Query: 437 TYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPD 496
            YEPPR G+TLWEIG+PDRSAAEFY+PDPNP++VNKLYLNH DK+RQYGLWERYSEL+PD
Sbjct: 421 VYEPPRDGSTLWEIGVPDRSAAEFYIPDPNPSFVNKLYLNHSDKYRQYGLWERYSELYPD 480

Query: 497 SDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT--YKLRVALAS 556
            D+VY V + DY+K+WFF QV RK  NG YKGTTWQ++F+ DD     T  +KLR+ALA+
Sbjct: 481 EDMVYNVDIDDYSKNWFFMQVTRKQANGGYKGTTWQIRFQFDDKMKNVTGNFKLRIALAT 540

Query: 557 VSLAELQVGVNEAKA-KAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIF 616
            ++AELQV VN+  A   +F +  IGRDN+IARHGIHGLYW+Y++++P   L  GNNTI+
Sbjct: 541 SNVAELQVRVNDLSADPPLFRTEQIGRDNTIARHGIHGLYWLYSVNVPAASLHVGNNTIY 600

Query: 617 FTQPRCTGPFQAIMYDYIRLEAP 626
            TQ   T PFQ +MYDYIRLE P
Sbjct: 601 LTQALATSPFQGLMYDYIRLEYP 622

[Close]
BLAST of Cucsat.G4655.T6 vs. TAIR 10
Match: AT2G22620.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 745.3 bits (1923), Expect = 3.9e-215
Identity = 350/633 (55.29%), Postives = 455/633 (71.88%), Query Frame = 0 

Query: 1   MPPLGVQL-FITERQVTMDNGIVQVTLSNPDGIVTGIRYGGVDNVLEVLNDESNRGYWDL 60
           +P L VQL  +    V +DNGIVQVT SNP+G++TGI+Y G+DNVL+   D+  RGYWD+
Sbjct: 47  IPALTVQLRRVGHDTVVVDNGIVQVTFSNPEGLITGIKYHGIDNVLDDKIDD--RGYWDV 106

Query: 61  VWSSSSSTTGLFDVIKATGFEVIVENEEQVELSFTRTWDLSLEGKLVPLEIDKRFIMLRG 120
           VW          D ++ T FE+I +NEEQ+E+SFTRTW +S  G LVPL +DKR+I+  G
Sbjct: 107 VWYEPEKKQKT-DKLEGTKFEIITQNEEQIEISFTRTWTISRRGSLVPLNVDKRYIIRSG 166

Query: 121 SSGFYSYAIYQHLEEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRYMPLPDDRSPPRGR 180
            SG Y Y I + LE WP  ++ + R+ FKL   KF +MA++D+RQR MP   DR     +
Sbjct: 167 VSGLYMYGILERLEGWPDVDMDQIRIVFKLNPKKFDFMAISDDRQRSMPSMADRE--NSK 226

Query: 181 ALAYPEAVLLVDPIEPELQGE------YSCDNKDSGVHGWISTDPAVGFWLISPSNEFRS 240
           +LAY EAVLL +P  P  +GE      YS ++KD+ VHGWIS+DP VGFW+I+PS+EFR 
Sbjct: 227 SLAYKEAVLLTNPSNPMFKGEVDDKYMYSMEDKDNNVHGWISSDPPVGFWMITPSDEFRL 286

Query: 241 GGPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDA 300
           GGP+KQ+LTSH GP TL++F S HYAG+++      GE WKKVFGPV  YLNS       
Sbjct: 287 GGPIKQDLTSHAGPITLSMFTSTHYAGKEMRMDYRNGEPWKKVFGPVLAYLNSVSPKDST 346

Query: 301 LQLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFV 360
           L+LW DAK Q   EV+SWPY F  S+D+P  +QR ++ G+ L+KDSYV+   I    AFV
Sbjct: 347 LRLWRDAKRQMAAEVKSWPYDFITSEDYPLRHQRGTLEGQFLIKDSYVSRLKIYGKFAFV 406

Query: 361 GLAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALIN 420
           GLAP G+ GSWQ ESKGYQFW++AD  G F I+ VR G+Y+LYAW  GFIGDY+Y+  I 
Sbjct: 407 GLAPIGEAGSWQTESKGYQFWTKADRRGRFIIENVRAGNYSLYAWGSGFIGDYKYEQNIT 466

Query: 421 LTEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNH-PDKFRQ 480
           +T GSE+++G + YEPPR G TLWEIG+PDR+A EFY+PDP P  +NKLY+N   D+FRQ
Sbjct: 467 ITPGSEMNVGPLVYEPPRNGPTLWEIGVPDRTAGEFYIPDPYPTLMNKLYVNPLQDRFRQ 526

Query: 481 YGLWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQ 540
           YGLW+RY++L+P +DLVYT+GVSDY  DWFFA V R + N TY+ TTWQ+ F L +V+  
Sbjct: 527 YGLWDRYADLYPQNDLVYTIGVSDYRSDWFFAHVARNVGNDTYQPTTWQIIFNLKNVNRI 586

Query: 541 ATYKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGN 600
             Y LR+ALAS + +ELQ+ +N+ K+ A+F++G IG+DN+IARHGIHGLY +Y+ID+ GN
Sbjct: 587 GRYTLRIALASAADSELQIRINDPKSDAIFTTGFIGKDNAIARHGIHGLYRLYSIDVAGN 646

Query: 601 RLVTGNNTIFFTQPRCTGPFQAIMYDYIRLEAP 626
            L  G+NTIF TQ R   PFQ IMYDYIRLE+P
Sbjct: 647 LLSVGDNTIFLTQTRSRTPFQGIMYDYIRLESP 674

The following BLAST results are available for this feature:
BLAST of Cucsat.G4655.T6 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
A5ABH43.1e-2326.02Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / F... [more]
Q5AZ853.8e-2123.30Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 ... [more]
Q2U5P72.0e-1423.44Probable rhamnogalacturonate lyase C OS=Aspergillus oryzae (strain ATCC 42149 / ... [more]
BLAST of Cucsat.G4655.T6 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004152608.20.099.06probable rhamnogalacturonate lyase B isoform X1 [Cucumis sativus] >KGN62737.1 hy... [more]
XP_008444895.10.095.49PREDICTED: probable rhamnogalacturonate lyase B isoform X1 [Cucumis melo][more]
KAA0065109.10.095.33putative rhamnogalacturonate lyase B isoform X1 [Cucumis melo var. makuwa][more]
XP_038886212.10.091.74probable rhamnogalacturonate lyase B isoform X1 [Benincasa hispida][more]
XP_023537007.10.088.28probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo] >XP... [more]
BLAST of Cucsat.G4655.T6 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LPL00.099.06Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_2G370370 PE=3 SV=... [more]
A0A1S3BCA70.095.49Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103488110 PE=3 SV=1[more]
A0A5A7VI090.095.33Rhamnogalacturonan endolyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A6J1KMB30.087.50Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111495919 PE=3 SV... [more]
A0A6J1GGG50.087.34Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111454003 PE=3 ... [more]
BLAST of Cucsat.G4655.T6 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G09890.15.0e-27168.88Rhamnogalacturonate lyase family protein [more]
AT1G09910.18.9e-26065.18Rhamnogalacturonate lyase family protein [more]
AT4G24430.11.2e-24862.34Rhamnogalacturonate lyase family protein [more]
AT1G09880.12.7e-24061.96Rhamnogalacturonate lyase family protein [more]
AT2G22620.13.9e-21555.29Rhamnogalacturonate lyase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.120.260coord: 432..623
e-value: 4.8E-20
score: 73.8
NoneNo IPR availableGENE3D2.60.40.1120coord: 325..421
e-value: 8.1E-8
score: 34.4
NoneNo IPR availablePANTHERPTHR32018:SF8RHAMNOGALACTURONATE LYASE FAMILY PROTEINcoord: 1..626
NoneNo IPR availablePANTHERPTHR32018RHAMNOGALACTURONATE LYASE FAMILY PROTEINcoord: 1..626
NoneNo IPR availableCDDcd10320RGL4_Ncoord: 13..287
e-value: 3.70239E-72
score: 231.894
NoneNo IPR availableCDDcd10317RGL4_Ccoord: 437..623
e-value: 9.62353E-54
score: 179.008
IPR010325Rhamnogalacturonate lyasePFAMPF06045Rhamnogal_lyasecoord: 11..200
e-value: 2.5E-89
score: 298.3
IPR029411Rhamnogalacturonan lyase, domain IIIPFAMPF14683CBM-likecoord: 435..622
e-value: 4.6E-57
score: 192.6
IPR014718Glycoside hydrolase-type carbohydrate-bindingGENE3D2.70.98.10coord: 7..277
e-value: 2.1E-5
score: 26.1
IPR029413Rhamnogalacturonan lyase, domain IIPFAMPF14686fn3_3coord: 349..420
e-value: 4.7E-24
score: 84.0
IPR029413Rhamnogalacturonan lyase, domain IICDDcd10316RGL4_Mcoord: 326..425
e-value: 8.55407E-31
score: 113.498
IPR011013Galactose mutarotase-like domain superfamilySUPERFAMILY74650Galactose mutarotase-likecoord: 19..286
IPR008979Galactose-binding-like domain superfamilySUPERFAMILY49785Galactose-binding domain-likecoord: 433..623
IPR013784Carbohydrate-binding-like foldSUPERFAMILY49452Starch-binding domain-likecoord: 349..421

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G4655Cucsat.G4655gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G4655.T6.E1Cucsat.G4655.T6.E1exon
Cucsat.G4655.T6.E2Cucsat.G4655.T6.E2exon
Cucsat.G4655.T6.E3Cucsat.G4655.T6.E3exon
Cucsat.G4655.T6.E4Cucsat.G4655.T6.E4exon
Cucsat.G4655.T6.E5Cucsat.G4655.T6.E5exon
Cucsat.G4655.T6.E6Cucsat.G4655.T6.E6exon
Cucsat.G4655.T6.E7Cucsat.G4655.T6.E7exon
Cucsat.G4655.T6.E8Cucsat.G4655.T6.E8exon
Cucsat.G4655.T6.E9Cucsat.G4655.T6.E9exon
Cucsat.G4655.T6.E10Cucsat.G4655.T6.E10exon
Cucsat.G4655.T6.E11Cucsat.G4655.T6.E11exon
Cucsat.G4655.T6.E12Cucsat.G4655.T6.E12exon
Cucsat.G4655.T6.E13Cucsat.G4655.T6.E13exon
Cucsat.G4655.T6.E14Cucsat.G4655.T6.E14exon
Cucsat.G4655.T6.E15Cucsat.G4655.T6.E15exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G4655.T6.C1Cucsat.G4655.T6.C1CDS
Cucsat.G4655.T6.C2Cucsat.G4655.T6.C2CDS
Cucsat.G4655.T6.C3Cucsat.G4655.T6.C3CDS
Cucsat.G4655.T6.C4Cucsat.G4655.T6.C4CDS
Cucsat.G4655.T6.C5Cucsat.G4655.T6.C5CDS
Cucsat.G4655.T6.C6Cucsat.G4655.T6.C6CDS
Cucsat.G4655.T6.C7Cucsat.G4655.T6.C7CDS
Cucsat.G4655.T6.C8Cucsat.G4655.T6.C8CDS
Cucsat.G4655.T6.C9Cucsat.G4655.T6.C9CDS
Cucsat.G4655.T6.C10Cucsat.G4655.T6.C10CDS
Cucsat.G4655.T6.C11Cucsat.G4655.T6.C11CDS
Cucsat.G4655.T6.C12Cucsat.G4655.T6.C12CDS
Cucsat.G4655.T6.C13Cucsat.G4655.T6.C13CDS
Cucsat.G4655.T6.C14Cucsat.G4655.T6.C14CDS
Cucsat.G4655.T6.C15Cucsat.G4655.T6.C15CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G4655.T6Cucsat.G4655.T6-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0102210 rhamnogalacturonan endolyase activity