Cucsat.G4655.T4 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G4655.T4
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Rhamnogalacturonan endolyase
Location	ctg1227: 1348226 .. 1351743 (+)
RNA-Seq Expression	Cucsat.G4655.T4
Synteny	Cucsat.G4655.T4

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTATTTTCTTTTTTATAGAGAAAACCAGAATCATGCTAACGTAAATGAGTTTTGATTCCTCTAATTTCATATGGACTGTAAAGGTGGATGACAAATATCAGTACTCATGCGACAACAAAGACAGCGGCGTCCATGGATGGATATCCACCGATCCGGCAGTCGGATTCTGGCTAATCTCCCCGAGCAATGAGTTCCGATCCGGTGGACCCGTTAAACAGAACCTAACTTCACACGTCGGCCCAACCACCCTAGCTGTAAGTGATTCTTCGTTCATTCAGCCATCAATCGTTATCCTTGGCTTTGATGTTCGATATAATTGGAATTAAGCCAGATTATACATTTACCTTAACTATGGAATCAGGTTTTTATAAGCGCACATTACGCGGGAGAGGATCTGGTGCCGAAAATCGGAGCCGGCGAGGCGTGGAAGAAAGTTTTCGGTCCTGTGTTCATCTATCTCAACTCCACTTATGATTCCTTCGACGCACTTCAGCTCTGGGAAGATGCCAAAGCACAGGTTTCTTCTATTTTTCTTCATTTCCATTCAATGACAATTCTTTTCTCGCGTCTTCTTTACCCACATCCAAATTTCTGGGTTTTTTTTTTTTTTTTTTGAATTATTCCAGTTCCTAAGATGGGTAATTTGACCTGTCTACTATCTTTTTACACAAAAAATAGACAATCTATTATTATATATATGTGTTTGCCACACGGTGTAAGTTTATTAAAAAAATTATTTGGACTTTACCAAGATAAGCGGCTGCCTGCAATCGAACCTGCGCCTAAGTTTTTCTTCTTGTTTGTTCATCAATTATTTTAAGTTCCAACATATCCATAAAATTTGTAGTACTTTCAATTTTGTCTCAGTACTAGTAAAGGTGTATGGATTAAATTTGAAAAAATAGATTTTAGTCAAAAGTTTTGGCAAGAAAACTTTTCCTGTGACTTACTTTGAGTTACTCTTAAAATCAAATAAAATTTAAGAAATATTTTTCCCCATGAAAAGGCAGTGATCGAAGTCCAGAGTTGGCCCTACAGCTTCCCTGCTTCGGATGATTTTCCAAGGTCAAACCAACGTAGTAGTATCAGTGGTAAACTATTAGTCAAGGATAGGTACACAATTGCCTCAACATTATATTTAAAATGTTTATGATGGAAATTCTGGTGAATTTTAGTGCTTCATCCAAAACTAATTGGCTAATTTAAATATCGCGACTTGATTTTAGCTACGTCAATGAAGACTTGATACCAGCAGACGGTGCGTTTGTTGGCCTGGCTCCACCAGGAGACGTAGGATCATGGCAACGAGAGAGCAAGGTAAAATAATTTAATTTTCTTGTTTAACCAATAAATTTTATAGAATTATTTTGATGGTATCGTCCAAAATTTTTTAGGGTTACCAATTTTGGAGTAGAGCAGATGAAAGTGGAAATTTCTCCATCGATGGCGTGAGGCCGGGCGACTACAATCTGTATGCATGGGTCCCCGGTTTTATTGGAGATTATCGTTATGATGCTCTCATCAACCTTACAGAAGGTTCCAATTTTCTTACTCTATGGCAATAAAATTGGTTAGAATCTCTAGTTTAGAAAAGTTAATGGGACAATCTGATGTTTCTATTGAAAAATATATAAACATTTCTCTTCCAAATTTCCAATGGACCTTAGCGTAAATGCATTTTGGTGTCTGAGTTTGCATATTCAATATTCAAACTTCAGTTTGACCTCTTCCATGAGGTTATTAACTTTAAATTTTAATTGATCTAAGAGAAAATTAATTAATTGTTGCCAAAATAAGTATAATTTAATTATAAAAAATGTTCAAATCAACCAACATATTTACTAGATATAACAAAATTTTAGATCTAACTAGTTAATACTAGTTACTATCGAATTAATTAGAAATCCTCTCATGGTTGAAAAAAATCTCTCACTCATAGTGGTATAAAGTTGAATAGATCCAAGATTCATTTGTTGGGTTGTTTTGTACCAATCCATTTCTATCTGTACTATATGTCTGTTTTTTTCTTCTAAATTTGGACAAGCAATTATAGGTTAATTTTGTTAATATACGATAATTTATGTAGGCAGTGAGGTGGACATGGGAGACATAACATACGAACCTCCGAGACAAGGAGCCACACTATGGGAAATAGGAATCCCAGATCGCTCCGCAGCAGAGTTCTACGTTCCAGATCCAAATCCCAACTACGTCAATAAGCTCTACCTTAACCATCCTGATAAGTAAATGAACCTACTTCATATTTCAAGCCCACTGATTTTTCATTATTCCCCTTTAATCGAATCAAAAGATTTGGTGAATCCTTGTACCAAGTCTTGATGAGATATCACTTTGATAAAATTACTCCCACGTTCTTAATTAATCTGATAAAGGATTTGACAGGTTTCGGCAGTATGGCCTATGGGAAAGATACAGTGAATTGTTTCCCGACTCAGATTTGGTATATACCGTTGGCGTTAGTGACTACACCAAGGATTGGTTCTTTGCTCAGGTCCCCAGGTGTGTGCTTCATTAATTTTTCCGATCATCCAGAAATAATTTCAAACTTGTTTGGTTTGATTATTAAAATGGAATTCCATAATTAAACTGATAAACCAAAAACCTGGGAGATACGAACAGTATAGTATCCATCTGTTTGACAGCTTCTTTTCACCTACAGGAAGATGGAAAATGGGACATATAAGGGAACAACGTGGCAAGTGAAGTTCGAGCTAGACGACGTGGACAACCAAGCAACTTACAAGCTTCGGGTTGCATTGGCCTCCGTTTCTCTCGCAGAATTGCAGGTATACCAAATTTCAACGTTTCCTTTTCCTAAAAAGTTGAATTGCCAAATCATATTGTTTCTAAACGTTGCACTTTCTTAATAGCAGTAAGATGTTTATCTGATTAGTTAGAAGCCTAGTAACAGTGAATGAAAGGAAAGTTTTGAGTTTTGAAGGTTGGAGTTAACGAGGCCAAAGCAAAGGCAGTGTTCTCGAGTGGACTTATCGGGAGGGACAATTCGATTGCAAGACACGGCATTCACGGCCTATATTGGGTTTACAACATTGACATTCCTGGAAATCGGCTGGTCACCGGCAATAACACCATCTTCTTCACCCAACCCAGATGCACAGGCCCTTTCCAAGCGATTATGTACGATTACATTCGTCTGGAAGCTCCTCCACCTCCATCCTCCGCCCATCCTTTGGAATAATATTCCCCTACCACACGTCCTTCCCTTCCACATCCTTAATTTCCACCATTTGCTTTCTTGTTTCGTTTTCTTTATACACAATCAATTTCGCTAACTTAAAAACTGTACTCGTAACCAGGGTAGGATCTTTCCTAAGGGGTTTAGAATGAGATTCAGACTTTTAGAATTGATAAAGTTCATAATAAAACCTATGTCGTGAAACTACTACAATCTCATGTGTCGTGAAAAATGCAAAACCAAAAAACAATTTTATAGACATCATATTATTCAGTACAAAATATGTACAAAATATTGGGA

Coding sequence (CDS)

ATGAGTTTTGATTCCTCTAATTTCATATGGACTGTAAAGGTGGATGACAAATATCAGTACTCATGCGACAACAAAGACAGCGGCGTCCATGGATGGATATCCACCGATCCGGCAGTCGGATTCTGGCTAATCTCCCCGAGCAATGAGTTCCGATCCGGTGGACCCGTTAAACAGAACCTAACTTCACACGTCGGCCCAACCACCCTAGCTGTTTTTATAAGCGCACATTACGCGGGAGAGGATCTGGTGCCGAAAATCGGAGCCGGCGAGGCGTGGAAGAAAGTTTTCGGTCCTGTGTTCATCTATCTCAACTCCACTTATGATTCCTTCGACGCACTTCAGCTCTGGGAAGATGCCAAAGCACAGGCAGTGATCGAAGTCCAGAGTTGGCCCTACAGCTTCCCTGCTTCGGATGATTTTCCAAGGTCAAACCAACGTAGTAGTATCAGTGGTAAACTATTAGTCAAGGATAGCTACGTCAATGAAGACTTGATACCAGCAGACGGTGCGTTTGTTGGCCTGGCTCCACCAGGAGACGTAGGATCATGGCAACGAGAGAGCAAGGGTTACCAATTTTGGAGTAGAGCAGATGAAAGTGGAAATTTCTCCATCGATGGCGTGAGGCCGGGCGACTACAATCTGTATGCATGGGTCCCCGGTTTTATTGGAGATTATCGTTATGATGCTCTCATCAACCTTACAGAAGGCAGTGAGGTGGACATGGGAGACATAACATACGAACCTCCGAGACAAGGAGCCACACTATGGGAAATAGGAATCCCAGATCGCTCCGCAGCAGAGTTCTACGTTCCAGATCCAAATCCCAACTACGTCAATAAGCTCTACCTTAACCATCCTGATAAGTTTCGGCAGTATGGCCTATGGGAAAGATACAGTGAATTGTTTCCCGACTCAGATTTGGTATATACCGTTGGCGTTAGTGACTACACCAAGGATTGGTTCTTTGCTCAGGTCCCCAGGAAGATGGAAAATGGGACATATAAGGGAACAACGTGGCAAGTGAAGTTCGAGCTAGACGACGTGGACAACCAAGCAACTTACAAGCTTCGGGTTGCATTGGCCTCCGTTTCTCTCGCAGAATTGCAGGTTGGAGTTAACGAGGCCAAAGCAAAGGCAGTGTTCTCGAGTGGACTTATCGGGAGGGACAATTCGATTGCAAGACACGGCATTCACGGCCTATATTGGGTTTACAACATTGACATTCCTGGAAATCGGCTGGTCACCGGCAATAACACCATCTTCTTCACCCAACCCAGATGCACAGGCCCTTTCCAAGCGATTATGTACGATTACATTCGTCTGGAAGCTCCTCCACCTCCATCCTCCGCCCATCCTTTGGAATAA

Protein sequence

MSFDSSNFIWTVKVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQAIMYDYIRLEAPPPPSSAHPLE

Homology

[Close]

BLAST of Cucsat.G4655.T4 vs. ExPASy Swiss-Prot
Match: A5ABH4 (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 2.6e-24
Identity = 124/473 (26.22%), Postives = 190/473 (40.17%), Query Frame = 0


Query: 17  KYQYSCDNKDSGVHGW-----ISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAV 76
           KY +S   +D+ VHG       S     G WL+  + +   GGP+  +LT  V       
Sbjct: 216 KYTFSNAWRDNSVHGMYADGSTSNGSTFGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNY 275

Query: 77  FISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQ-LWEDAKAQAVIEVQSW 136
            +S H+ GE   P I  G  + + FGP + + N    S  +LQ L  DA+  A     SW
Sbjct: 276 LVSNHH-GEG-TPNITYG--FDRTFGPQYYHFNGGKGSTASLQELKSDAETLA---DPSW 335

Query: 137 PYSFPAS-----DDFPRSNQRSSISGKL-LVKDSYVNEDLIPADGAFVGLAPPGDVGSWQ 196
              F  S       +  S+QR S+ GK+ L K +     ++  DG +             
Sbjct: 336 NVDFYDSIAKHVVGYTPSSQRGSVQGKIKLPKGATRPIAVLTVDGQY--------FQDNS 395

Query: 197 RESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDI 256
             S  YQ+W+  D+SG+FS+D V+ G Y L  +  G  GD+  D  + +  G +  + + 
Sbjct: 396 VNSSSYQYWAEIDDSGHFSVDHVKEGPYRLTVYADGIFGDFVRDG-VQVKAGKKTTIQE- 455

Query: 257 TYEPPRQGATLWEIGIPDRSAAEF---YVPDPNPNYVNKLYLNHPDKFRQYGLWERYSEL 316
           T+E    G  +W +G PD+S+ EF      DP         L+ P+    +G ++  S+ 
Sbjct: 456 TWEAESAGTEIWRLGTPDKSSGEFRHGVARDPTHP------LHPPEYLIYWGAYDWQSD- 515

Query: 317 FPDSDLVYTVGVSDYTKD-----W-FFAQVPRKMENGTYKGTTWQVKFEL--DDVDNQAT 376
           FPD  + YT+G SD   D     W  F   P            W + F L  DD+  ++ 
Sbjct: 516 FPDG-INYTIGTSDPATDLNTVHWSVFGPTPNDPRVEYDTTHDWTINFPLSEDDLAERSK 575

Query: 377 YKLRVALASVSLAE----------------LQVGVNEAKAKAVFSSGLIGRDNSIARHGI 436
             L + LA    A                 L+  +N+         G     + I R  +
Sbjct: 576 ATLTIQLAGAKAASGNTDVYNASEPYTNLALESYINDQAEPLTLLIGFNQSSSCIVRSAV 635

Query: 437 HGLYWVYNIDIPGNRLVTGNNTIFFTQP-RCTGPFQAIMYDYI--RLEAPPPP 448
                   ++ P + L  GNN +    P   T    AI+   +  RL  PP P
Sbjct: 636 SCYQVRSRMEFPADWLKVGNNVLTLHLPYNATDTETAILPATVTGRLILPPQP 661

[Close]

BLAST of Cucsat.G4655.T4 vs. ExPASy Swiss-Prot
Match: Q5AZ85 (Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2)

HSP 1 Score: 98.2 bits (243), Expect = 2.5e-19
Identity = 115/476 (24.16%), Postives = 192/476 (40.34%), Query Frame = 0


Query: 17  KYQYSCDNKDSGVHGW-----ISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAV 76
           KY +S   +D+ VHG       S     G WL+  + +   GGP+  +LT  V       
Sbjct: 215 KYTFSNHWRDNDVHGLYADGSTSDGTTYGAWLVMNTKDTYYGGPLHSDLT--VDGIIYNY 274

Query: 77  FISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWP 136
            +S H+ GE   P I  G  + + FGP F YL +   S    +L ++A++ A     SW 
Sbjct: 275 IVSNHH-GEG-TPNITNG--FDRTFGPQF-YLFNGGGSSSLEELRDEARSLA---SPSWN 334

Query: 137 YSFPAS-----DDFPRSNQRSSISGKL-LVKDSYVNEDLIPADGAFVGLAPPGDVGSWQR 196
             F  S       +  S+QR S+ G + L K++     ++  DG +           +Q 
Sbjct: 335 ADFYDSIAKHVIGYVPSSQRGSVKGTIKLPKNAKSPIAVLTVDGHY-----------FQD 394

Query: 197 ES---KGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMG 256
            S     +Q+W+  D++G FSID V  G Y L  +  G  GD+  D ++   +  +    
Sbjct: 395 NSAVPSSHQYWADIDKNGRFSIDRVVAGKYRLTVYADGIFGDFTRDGIV--VKARKSTSI 454

Query: 257 DITYEPPRQGATLWEIGIPDRSAAEF---YVPDPNPNYVNKLYLNHPDKFRQY-GLWERY 316
             T++P   G  +W +G PD+S+ EF      DP        +  HP ++  Y G ++  
Sbjct: 455 KETWKPESAGTEIWRLGTPDKSSGEFRHGAARDPT-------HPRHPPEYLIYWGAYDWQ 514

Query: 317 SELFPDSDLVYTVGVSDYTKDW------FFAQVPRKMENGTYKGTTWQVKFELD--DVDN 376
           S+ FP   + Y +G SD   D+       F   P      +     W+++F+L    +  
Sbjct: 515 SD-FP-GGIDYMIGESDPATDFNTVHWAVFGPTPDNPVAESNTTHDWRIRFDLSAKQLHA 574

Query: 377 QATYKLRVALASVSLAE----------------LQVGVNEAKAKAVFSSGLIGRDNSIAR 436
           + T  L + LA    A                 L+  +NE +       G     + I R
Sbjct: 575 RKTATLTIQLAGAKAASGNTDVYNASEPYANLPLRSYINEQEEPLTMVIGYDQSSSCIVR 634

Query: 437 HGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQA--------IMYDYIRLE 443
             +         + P + L  G+N +  + P     +++        + YD +RLE
Sbjct: 635 SAVSCYQVREKWEFPASWLKEGSNLLRLSLPTNGTNYESAVLPTSVYVQYDALRLE 658

[Close]

BLAST of Cucsat.G4655.T4 vs. ExPASy Swiss-Prot
Match: Q2U5P7 (Probable rhamnogalacturonate lyase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rglC PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 7.1e-14
Identity = 91/372 (24.46%), Postives = 142/372 (38.17%), Query Frame = 0


Query: 17  KYQYSCDNKDSGVHGWISTDPA------VGFWLISPSNEFRSGGPVKQNLTSHVGPTTLA 76
           KY  +   ++  VHG  S   +       G WL+  + E   GGP+  +L   V      
Sbjct: 215 KYTLAESWRNHDVHGLFSDGSSSSDGSTFGAWLVHNTVETYYGGPLHSDLV--VDGIVYN 274

Query: 77  VFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSW 136
             +S H+      P       + + +GP F Y N          L  DA   A  E  + 
Sbjct: 275 YLVSGHHG----APTPNLTHGFDRTWGPQFYYFNRGDSETTLADLRADAAKYADPEWNAE 334

Query: 137 PYSFPAS--DDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPG-DVGSWQRES 196
            Y   A    +F  S  R++  GK+ +           A    + L+  G D       +
Sbjct: 335 FYDSIADHIPNFTPSTGRTTFKGKVSLPKG--------AKRPIIVLSEDGQDFQLNVFNT 394

Query: 197 KGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYE 256
           +  Q+W+  D+SG+FSI  V  G Y +  +     G +  D  + + +    D    T++
Sbjct: 395 ESLQYWAEIDKSGSFSIPRVVEGTYRITIYADEIFGWFIQDH-VKVLKSQSKDY-SFTWK 454

Query: 257 PPRQGATLWEIGIPDRSAAEF---YVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPD 316
               G  +W IGIPD+S+ E+   Y PD +           P++ R Y     Y   FP+
Sbjct: 455 EESAGKEIWRIGIPDKSSGEYLHGYAPDTSKPL-------QPEQHRIYWGKYDYPADFPE 514

Query: 317 SDLVYTVGVSDYTKD-----W-FFAQVPRKMENGTY--KGTTWQVKFEL--DDVDNQATY 367
             + + VG SD ++D     W FF      +    Y      W V F+L  D + N  T 
Sbjct: 515 G-INFHVGKSDPSQDLNYIHWAFFPSQGNHLRTEPYYDNVNNWTVTFDLTADQLHNTNTA 562

[Close]

BLAST of Cucsat.G4655.T4 vs. NCBI nr
Match: XP_031736466.1 (probable rhamnogalacturonate lyase B isoform X2 [Cucumis sativus])

HSP 1 Score: 929 bits (2400), Expect = 0.0
Identity = 441/442 (99.77%), Postives = 442/442 (100.00%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 72
           +VDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF
Sbjct: 85  EVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 144

Query: 73  ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 132
           ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY
Sbjct: 145 ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 204

Query: 133 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 192
           SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF
Sbjct: 205 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 264

Query: 193 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 252
           WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG
Sbjct: 265 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 324

Query: 253 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 312
           ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG
Sbjct: 325 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 384

Query: 313 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 372
           VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV
Sbjct: 385 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 444

Query: 373 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ 432
           NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ
Sbjct: 445 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ 504

Query: 433 AIMYDYIRLEAPPPPSSAHPLE 454
           AIMYDYIRLEAPPPPSSAHPLE
Sbjct: 505 AIMYDYIRLEAPPPPSSAHPLE 526

[Close]

BLAST of Cucsat.G4655.T4 vs. NCBI nr
Match: XP_004152608.2 (probable rhamnogalacturonate lyase B isoform X1 [Cucumis sativus] >KGN62737.1 hypothetical protein Csa_022438 [Cucumis sativus])

HSP 1 Score: 929 bits (2400), Expect = 0.0
Identity = 441/442 (99.77%), Postives = 442/442 (100.00%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 72
           +VDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF
Sbjct: 200 EVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 259

Query: 73  ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 132
           ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY
Sbjct: 260 ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 319

Query: 133 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 192
           SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF
Sbjct: 320 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 379

Query: 193 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 252
           WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG
Sbjct: 380 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 439

Query: 253 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 312
           ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG
Sbjct: 440 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 499

Query: 313 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 372
           VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV
Sbjct: 500 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 559

Query: 373 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ 432
           NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ
Sbjct: 560 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ 619

Query: 433 AIMYDYIRLEAPPPPSSAHPLE 454
           AIMYDYIRLEAPPPPSSAHPLE
Sbjct: 620 AIMYDYIRLEAPPPPSSAHPLE 641

[Close]

BLAST of Cucsat.G4655.T4 vs. NCBI nr
Match: XP_008444895.1 (PREDICTED: probable rhamnogalacturonate lyase B isoform X1 [Cucumis melo])

HSP 1 Score: 897 bits (2318), Expect = 0.0
Identity = 426/444 (95.95%), Postives = 433/444 (97.52%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 72
           +VDDKYQYSCDNKDSGVHGWISTDP VGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF
Sbjct: 200 EVDDKYQYSCDNKDSGVHGWISTDPTVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 259

Query: 73  ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 132
           ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQA +EVQSWPY
Sbjct: 260 ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAAVEVQSWPY 319

Query: 133 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 192
           SFPASDDFPRSNQR SISGKLLVKDSYV+EDLIPA GAFVGLAPPGDVGSWQRESKGYQF
Sbjct: 320 SFPASDDFPRSNQRGSISGKLLVKDSYVDEDLIPAGGAFVGLAPPGDVGSWQRESKGYQF 379

Query: 193 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 252
           WSRADESGNF IDGVRPGDYNLYAWVPGFIGDYRYDALINL EGSEVDMGDITYEPPRQG
Sbjct: 380 WSRADESGNFFIDGVRPGDYNLYAWVPGFIGDYRYDALINLKEGSEVDMGDITYEPPRQG 439

Query: 253 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 312
           ATLWEIGIPDRSAAEFYVPDPNPNY+NKLYLNHPDKFRQYGLWERYSELFPDSDLVY VG
Sbjct: 440 ATLWEIGIPDRSAAEFYVPDPNPNYINKLYLNHPDKFRQYGLWERYSELFPDSDLVYNVG 499

Query: 313 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 372
           VSDYTKDWFFAQVPRK+ENGTYKGTTWQVKFELD+VDNQATYKLRVALAS SLAELQVGV
Sbjct: 500 VSDYTKDWFFAQVPRKIENGTYKGTTWQVKFELDNVDNQATYKLRVALASASLAELQVGV 559

Query: 373 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ 432
           NEAKAKAVFSSGLIGRDNSIARHGIHGLYW+YNIDIPGNRLVTGNNTIFFTQPR TGPFQ
Sbjct: 560 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWLYNIDIPGNRLVTGNNTIFFTQPRSTGPFQ 619

Query: 433 AIMYDYIRLEAPPPP--SSAHPLE 454
           AIMYDYIRLEAPPPP  SSAHPLE
Sbjct: 620 AIMYDYIRLEAPPPPPPSSAHPLE 643

[Close]

BLAST of Cucsat.G4655.T4 vs. NCBI nr
Match: XP_008444896.1 (PREDICTED: probable rhamnogalacturonate lyase B isoform X2 [Cucumis melo])

HSP 1 Score: 897 bits (2318), Expect = 0.0
Identity = 426/444 (95.95%), Postives = 433/444 (97.52%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 72
           +VDDKYQYSCDNKDSGVHGWISTDP VGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF
Sbjct: 152 EVDDKYQYSCDNKDSGVHGWISTDPTVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 211

Query: 73  ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 132
           ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQA +EVQSWPY
Sbjct: 212 ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAAVEVQSWPY 271

Query: 133 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 192
           SFPASDDFPRSNQR SISGKLLVKDSYV+EDLIPA GAFVGLAPPGDVGSWQRESKGYQF
Sbjct: 272 SFPASDDFPRSNQRGSISGKLLVKDSYVDEDLIPAGGAFVGLAPPGDVGSWQRESKGYQF 331

Query: 193 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 252
           WSRADESGNF IDGVRPGDYNLYAWVPGFIGDYRYDALINL EGSEVDMGDITYEPPRQG
Sbjct: 332 WSRADESGNFFIDGVRPGDYNLYAWVPGFIGDYRYDALINLKEGSEVDMGDITYEPPRQG 391

Query: 253 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 312
           ATLWEIGIPDRSAAEFYVPDPNPNY+NKLYLNHPDKFRQYGLWERYSELFPDSDLVY VG
Sbjct: 392 ATLWEIGIPDRSAAEFYVPDPNPNYINKLYLNHPDKFRQYGLWERYSELFPDSDLVYNVG 451

Query: 313 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 372
           VSDYTKDWFFAQVPRK+ENGTYKGTTWQVKFELD+VDNQATYKLRVALAS SLAELQVGV
Sbjct: 452 VSDYTKDWFFAQVPRKIENGTYKGTTWQVKFELDNVDNQATYKLRVALASASLAELQVGV 511

Query: 373 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ 432
           NEAKAKAVFSSGLIGRDNSIARHGIHGLYW+YNIDIPGNRLVTGNNTIFFTQPR TGPFQ
Sbjct: 512 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWLYNIDIPGNRLVTGNNTIFFTQPRSTGPFQ 571

Query: 433 AIMYDYIRLEAPPPP--SSAHPLE 454
           AIMYDYIRLEAPPPP  SSAHPLE
Sbjct: 572 AIMYDYIRLEAPPPPPPSSAHPLE 595

[Close]

BLAST of Cucsat.G4655.T4 vs. NCBI nr
Match: KAA0065109.1 (putative rhamnogalacturonate lyase B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 893 bits (2307), Expect = 0.0
Identity = 425/444 (95.72%), Postives = 431/444 (97.07%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 72
           +VDDKYQYSCDNKDSGVHGWISTDP VGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF
Sbjct: 200 EVDDKYQYSCDNKDSGVHGWISTDPTVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 259

Query: 73  ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 132
           ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQA IEVQSWPY
Sbjct: 260 ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAAIEVQSWPY 319

Query: 133 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 192
           SFPASDDFPRSNQR SISGKLLVKDSYV+EDLIPA GAFVGLAPPGDVGSWQRESKGYQF
Sbjct: 320 SFPASDDFPRSNQRGSISGKLLVKDSYVDEDLIPAGGAFVGLAPPGDVGSWQRESKGYQF 379

Query: 193 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 252
           WSRADESGNF IDG RPGDYNLYAWVPGFIGDYRYDALINL EGSEVDMGDITYEPPRQG
Sbjct: 380 WSRADESGNFFIDGARPGDYNLYAWVPGFIGDYRYDALINLKEGSEVDMGDITYEPPRQG 439

Query: 253 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 312
           ATLWEIGIPDRSAAEFYVPDPNPNY+NKLYLNHPDKFRQYGLWERYSELFPDSDLVY VG
Sbjct: 440 ATLWEIGIPDRSAAEFYVPDPNPNYINKLYLNHPDKFRQYGLWERYSELFPDSDLVYNVG 499

Query: 313 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 372
           VSDYTKDWFFAQVPR +ENGTYKGTTWQVKFELD+VDNQATYKLRVALAS SLAELQVGV
Sbjct: 500 VSDYTKDWFFAQVPRCIENGTYKGTTWQVKFELDNVDNQATYKLRVALASASLAELQVGV 559

Query: 373 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ 432
           NEAKAKAVFSSGLIGRDNSIARHGIHGLYW+YNIDIPGNRLVTGNNTIFFTQPR TGPFQ
Sbjct: 560 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWLYNIDIPGNRLVTGNNTIFFTQPRSTGPFQ 619

Query: 433 AIMYDYIRLEAPPPP--SSAHPLE 454
           AIMYDYIRLEAPPPP  SSAHPLE
Sbjct: 620 AIMYDYIRLEAPPPPPPSSAHPLE 643

[Close]

BLAST of Cucsat.G4655.T4 vs. ExPASy TrEMBL
Match: A0A0A0LPL0 (Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_2G370370 PE=3 SV=1)

HSP 1 Score: 929 bits (2400), Expect = 0.0
Identity = 441/442 (99.77%), Postives = 442/442 (100.00%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 72
           +VDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF
Sbjct: 200 EVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 259

Query: 73  ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 132
           ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY
Sbjct: 260 ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 319

Query: 133 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 192
           SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF
Sbjct: 320 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 379

Query: 193 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 252
           WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG
Sbjct: 380 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 439

Query: 253 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 312
           ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG
Sbjct: 440 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 499

Query: 313 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 372
           VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV
Sbjct: 500 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 559

Query: 373 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ 432
           NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ
Sbjct: 560 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ 619

Query: 433 AIMYDYIRLEAPPPPSSAHPLE 454
           AIMYDYIRLEAPPPPSSAHPLE
Sbjct: 620 AIMYDYIRLEAPPPPSSAHPLE 641

[Close]

BLAST of Cucsat.G4655.T4 vs. ExPASy TrEMBL
Match: A0A1S3BCA7 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103488110 PE=3 SV=1)

HSP 1 Score: 897 bits (2318), Expect = 0.0
Identity = 426/444 (95.95%), Postives = 433/444 (97.52%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 72
           +VDDKYQYSCDNKDSGVHGWISTDP VGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF
Sbjct: 200 EVDDKYQYSCDNKDSGVHGWISTDPTVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 259

Query: 73  ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 132
           ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQA +EVQSWPY
Sbjct: 260 ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAAVEVQSWPY 319

Query: 133 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 192
           SFPASDDFPRSNQR SISGKLLVKDSYV+EDLIPA GAFVGLAPPGDVGSWQRESKGYQF
Sbjct: 320 SFPASDDFPRSNQRGSISGKLLVKDSYVDEDLIPAGGAFVGLAPPGDVGSWQRESKGYQF 379

Query: 193 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 252
           WSRADESGNF IDGVRPGDYNLYAWVPGFIGDYRYDALINL EGSEVDMGDITYEPPRQG
Sbjct: 380 WSRADESGNFFIDGVRPGDYNLYAWVPGFIGDYRYDALINLKEGSEVDMGDITYEPPRQG 439

Query: 253 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 312
           ATLWEIGIPDRSAAEFYVPDPNPNY+NKLYLNHPDKFRQYGLWERYSELFPDSDLVY VG
Sbjct: 440 ATLWEIGIPDRSAAEFYVPDPNPNYINKLYLNHPDKFRQYGLWERYSELFPDSDLVYNVG 499

Query: 313 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 372
           VSDYTKDWFFAQVPRK+ENGTYKGTTWQVKFELD+VDNQATYKLRVALAS SLAELQVGV
Sbjct: 500 VSDYTKDWFFAQVPRKIENGTYKGTTWQVKFELDNVDNQATYKLRVALASASLAELQVGV 559

Query: 373 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ 432
           NEAKAKAVFSSGLIGRDNSIARHGIHGLYW+YNIDIPGNRLVTGNNTIFFTQPR TGPFQ
Sbjct: 560 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWLYNIDIPGNRLVTGNNTIFFTQPRSTGPFQ 619

Query: 433 AIMYDYIRLEAPPPP--SSAHPLE 454
           AIMYDYIRLEAPPPP  SSAHPLE
Sbjct: 620 AIMYDYIRLEAPPPPPPSSAHPLE 643

[Close]

BLAST of Cucsat.G4655.T4 vs. ExPASy TrEMBL
Match: A0A1S3BAY7 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103488110 PE=3 SV=1)

HSP 1 Score: 897 bits (2318), Expect = 0.0
Identity = 426/444 (95.95%), Postives = 433/444 (97.52%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 72
           +VDDKYQYSCDNKDSGVHGWISTDP VGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF
Sbjct: 152 EVDDKYQYSCDNKDSGVHGWISTDPTVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 211

Query: 73  ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 132
           ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQA +EVQSWPY
Sbjct: 212 ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAAVEVQSWPY 271

Query: 133 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 192
           SFPASDDFPRSNQR SISGKLLVKDSYV+EDLIPA GAFVGLAPPGDVGSWQRESKGYQF
Sbjct: 272 SFPASDDFPRSNQRGSISGKLLVKDSYVDEDLIPAGGAFVGLAPPGDVGSWQRESKGYQF 331

Query: 193 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 252
           WSRADESGNF IDGVRPGDYNLYAWVPGFIGDYRYDALINL EGSEVDMGDITYEPPRQG
Sbjct: 332 WSRADESGNFFIDGVRPGDYNLYAWVPGFIGDYRYDALINLKEGSEVDMGDITYEPPRQG 391

Query: 253 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 312
           ATLWEIGIPDRSAAEFYVPDPNPNY+NKLYLNHPDKFRQYGLWERYSELFPDSDLVY VG
Sbjct: 392 ATLWEIGIPDRSAAEFYVPDPNPNYINKLYLNHPDKFRQYGLWERYSELFPDSDLVYNVG 451

Query: 313 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 372
           VSDYTKDWFFAQVPRK+ENGTYKGTTWQVKFELD+VDNQATYKLRVALAS SLAELQVGV
Sbjct: 452 VSDYTKDWFFAQVPRKIENGTYKGTTWQVKFELDNVDNQATYKLRVALASASLAELQVGV 511

Query: 373 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ 432
           NEAKAKAVFSSGLIGRDNSIARHGIHGLYW+YNIDIPGNRLVTGNNTIFFTQPR TGPFQ
Sbjct: 512 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWLYNIDIPGNRLVTGNNTIFFTQPRSTGPFQ 571

Query: 433 AIMYDYIRLEAPPPP--SSAHPLE 454
           AIMYDYIRLEAPPPP  SSAHPLE
Sbjct: 572 AIMYDYIRLEAPPPPPPSSAHPLE 595

[Close]

BLAST of Cucsat.G4655.T4 vs. ExPASy TrEMBL
Match: A0A5A7VI09 (Rhamnogalacturonan endolyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004440 PE=3 SV=1)

HSP 1 Score: 893 bits (2307), Expect = 0.0
Identity = 425/444 (95.72%), Postives = 431/444 (97.07%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 72
           +VDDKYQYSCDNKDSGVHGWISTDP VGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF
Sbjct: 200 EVDDKYQYSCDNKDSGVHGWISTDPTVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 259

Query: 73  ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 132
           ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQA IEVQSWPY
Sbjct: 260 ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAAIEVQSWPY 319

Query: 133 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 192
           SFPASDDFPRSNQR SISGKLLVKDSYV+EDLIPA GAFVGLAPPGDVGSWQRESKGYQF
Sbjct: 320 SFPASDDFPRSNQRGSISGKLLVKDSYVDEDLIPAGGAFVGLAPPGDVGSWQRESKGYQF 379

Query: 193 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 252
           WSRADESGNF IDG RPGDYNLYAWVPGFIGDYRYDALINL EGSEVDMGDITYEPPRQG
Sbjct: 380 WSRADESGNFFIDGARPGDYNLYAWVPGFIGDYRYDALINLKEGSEVDMGDITYEPPRQG 439

Query: 253 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 312
           ATLWEIGIPDRSAAEFYVPDPNPNY+NKLYLNHPDKFRQYGLWERYSELFPDSDLVY VG
Sbjct: 440 ATLWEIGIPDRSAAEFYVPDPNPNYINKLYLNHPDKFRQYGLWERYSELFPDSDLVYNVG 499

Query: 313 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 372
           VSDYTKDWFFAQVPR +ENGTYKGTTWQVKFELD+VDNQATYKLRVALAS SLAELQVGV
Sbjct: 500 VSDYTKDWFFAQVPRCIENGTYKGTTWQVKFELDNVDNQATYKLRVALASASLAELQVGV 559

Query: 373 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ 432
           NEAKAKAVFSSGLIGRDNSIARHGIHGLYW+YNIDIPGNRLVTGNNTIFFTQPR TGPFQ
Sbjct: 560 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWLYNIDIPGNRLVTGNNTIFFTQPRSTGPFQ 619

Query: 433 AIMYDYIRLEAPPPP--SSAHPLE 454
           AIMYDYIRLEAPPPP  SSAHPLE
Sbjct: 620 AIMYDYIRLEAPPPPPPSSAHPLE 643

[Close]

BLAST of Cucsat.G4655.T4 vs. ExPASy TrEMBL
Match: A0A6J1GGJ8 (Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111454003 PE=3 SV=1)

HSP 1 Score: 827 bits (2135), Expect = 6.07e-300
Identity = 383/441 (86.85%), Postives = 415/441 (94.10%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 72
           +VDDKYQYSCDNKDSGVHGWISTDP +GFWLI+PSNE RSGGPVKQNLTSHVGPTTLAVF
Sbjct: 85  EVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSGGPVKQNLTSHVGPTTLAVF 144

Query: 73  ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 132
           ISAHY GEDLVPKIGAGEAWKKVFGPVFIYLNSTYDS DALQLWED K+QA IEVQSWPY
Sbjct: 145 ISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDALQLWEDGKSQASIEVQSWPY 204

Query: 133 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 192
           SFPAS+DFP+SNQR +ISG+LLVKDSYV+ED IPA+GAFVGLAPPGDVGSWQRESKGYQF
Sbjct: 205 SFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVGLAPPGDVGSWQRESKGYQF 264

Query: 193 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 252
           WSRADE G F ID +RPGDYNLYAWVPGFIGDYRYD++I++ EGS++D+ +I YEPPR G
Sbjct: 265 WSRADEDGYFFIDHIRPGDYNLYAWVPGFIGDYRYDSIIDILEGSQIDVDEIIYEPPRAG 324

Query: 253 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 312
           ATLWEIG+PDRSA EFYVPDPNPNYVNKLYLNH DKFRQYGLWERYSEL+PD+DLVYTVG
Sbjct: 325 ATLWEIGVPDRSAEEFYVPDPNPNYVNKLYLNHSDKFRQYGLWERYSELYPDADLVYTVG 384

Query: 313 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 372
           VSDYTKDWFFAQV RK ENGT+KGTTWQVKFELD+VD+QATYKLRVALAS S+AELQVGV
Sbjct: 385 VSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQATYKLRVALASASIAELQVGV 444

Query: 373 NEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPFQ 432
           N+ KAK VFSSGLIGRDNSIARHGIHGLYW+YN++IPGNRLV+G+NTIFFTQPR T PFQ
Sbjct: 445 NDPKAKPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRLVSGDNTIFFTQPRSTSPFQ 504

Query: 433 AIMYDYIRLEAPPPPSSAHPL 453
           AIMYDYIRLEAPPPPSSAH L
Sbjct: 505 AIMYDYIRLEAPPPPSSAHGL 525

[Close]

BLAST of Cucsat.G4655.T4 vs. TAIR 10
Match: AT1G09890.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 663.3 bits (1710), Expect = 1.4e-190
Identity = 295/434 (67.97%), Postives = 355/434 (81.80%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTD-PAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAV 72
           +VDDKYQYSC+NKD  VHGWI T+ P+VGFWLI+PS+E+R+GGP KQNLTSHVGPT LAV
Sbjct: 183 EVDDKYQYSCENKDITVHGWICTEQPSVGFWLITPSHEYRTGGPQKQNLTSHVGPTALAV 242

Query: 73  FISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLN-STYDSFDALQLWEDAKAQAVIEVQSW 132
           FISAHY GEDLVPK   GEAWKKVFGPVF+YLN ST D  D L LW+DAK+Q  +E +SW
Sbjct: 243 FISAHYTGEDLVPKFSEGEAWKKVFGPVFVYLNSSTDDDNDPLWLWQDAKSQMNVEAESW 302

Query: 133 PYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGY 192
           PYSFPASDD+ ++ QR ++ G+LLV+D YV++D I A+  +VGLA PG  GSWQRE K Y
Sbjct: 303 PYSFPASDDYVKTEQRGNVVGRLLVQDRYVDKDFIAANRGYVGLAVPGAAGSWQRECKEY 362

Query: 193 QFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPR 252
           QFW+R DE G F I G+RPG YNLYAW+PGFIGDY+YD +I +T G  + + D+ Y+PPR
Sbjct: 363 QFWTRTDEEGFFYISGIRPGQYNLYAWIPGFIGDYKYDDVITITSGCYIYVEDLVYQPPR 422

Query: 253 QGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYT 312
            GATLWEIG PDRSAAEFYVPDPNP Y+N LY NHPD+FRQYGLWERY+EL+PD DLVY 
Sbjct: 423 NGATLWEIGFPDRSAAEFYVPDPNPKYINNLYQNHPDRFRQYGLWERYAELYPDKDLVYV 482

Query: 313 VGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQV 372
           VG SDY KDWF+AQV RK +N TY+GTTWQ+KFEL ++D   +Y LRVA+AS + +ELQ+
Sbjct: 483 VGSSDYRKDWFYAQVTRKKDNKTYQGTTWQIKFELKNIDKNHSYTLRVAIASATFSELQI 542

Query: 373 GVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGP 432
            VN A A  +F+SGLIGRDNSIARHGIHGLYW++N+++ G++L+ G NT+F TQPR T P
Sbjct: 543 RVNNANASPMFTSGLIGRDNSIARHGIHGLYWLFNVEVAGSKLLEGENTLFLTQPRSTSP 602

Query: 433 FQAIMYDYIRLEAP 445
           FQ IMYDYIR EAP
Sbjct: 603 FQGIMYDYIRFEAP 616

[Close]

BLAST of Cucsat.G4655.T4 vs. TAIR 10
Match: AT1G09910.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 644.4 bits (1661), Expect = 6.7e-185
Identity = 285/434 (65.67%), Postives = 357/434 (82.26%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 72
           +VDDKYQYSC+NKD  VHGWIS DP VGFW I+PSNEFRSGGP+KQNLTSHVGPTTLAVF
Sbjct: 242 EVDDKYQYSCENKDLRVHGWISFDPPVGFWQITPSNEFRSGGPLKQNLTSHVGPTTLAVF 301

Query: 73  ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 132
            S HYAG+ ++P+   GE WKKV+GPVFIYLNST +  D L LW+DAK + + EV+ WPY
Sbjct: 302 HSTHYAGKTMMPRFEHGEPWKKVYGPVFIYLNSTANGDDPLCLWDDAKIKMMAEVERWPY 361

Query: 133 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 192
           SF ASDD+P+S +R +  G+LL++D ++N DLI A GA+VGLAPPGD GSWQ E KGYQF
Sbjct: 362 SFVASDDYPKSEERGTARGRLLIRDRFINNDLISARGAYVGLAPPGDSGSWQIECKGYQF 421

Query: 193 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 252
           W+ ADE+G FSI  VRPG+YNLYAWVP FIGDY    ++ +T G  ++MGDI YEPPR G
Sbjct: 422 WAIADEAGYFSIGNVRPGEYNLYAWVPSFIGDYHNGTIVRVTSGCMIEMGDIVYEPPRDG 481

Query: 253 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 312
            TLWEIGIPDR A+EF++PDP+P  VN++ ++H D+FRQYGLW++Y++++P+ DLVYTVG
Sbjct: 482 PTLWEIGIPDRKASEFFIPDPDPTLVNRVLVHHQDRFRQYGLWKKYTDMYPNDDLVYTVG 541

Query: 313 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVGV 372
           VSDY +DWFFA VPRK +   ++GTTWQ+ F L+++D +A YKLRVA+AS +LAELQ+ +
Sbjct: 542 VSDYRRDWFFAHVPRK-KGDVHEGTTWQIIFNLENIDQKANYKLRVAIASATLAELQIRI 601

Query: 373 NEAKA-KAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTGPF 432
           N+A+A + +F++GLIGRDNSIARHGIHG+Y +Y ++IPGNRLV G+NTIF  QPRC GPF
Sbjct: 602 NDAEAIRPLFTTGLIGRDNSIARHGIHGVYMLYAVNIPGNRLVQGDNTIFLKQPRCNGPF 661

Query: 433 QAIMYDYIRLEAPP 446
           Q IMYDYIRLE PP
Sbjct: 662 QGIMYDYIRLEGPP 674

[Close]

BLAST of Cucsat.G4655.T4 vs. TAIR 10
Match: AT4G24430.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 619.8 bits (1597), Expect = 1.8e-177
Identity = 280/444 (63.06%), Postives = 356/444 (80.18%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 72
           +VDDKY+YS +NKD  VHGWIS +  +G W I PSNEFRSGG  KQNLTSHVGP +LA+F
Sbjct: 203 EVDDKYEYSSENKDLKVHGWISHNLDLGCWQIIPSNEFRSGGLSKQNLTSHVGPISLAMF 262

Query: 73  ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYD-SFDALQLWEDAKAQAVIEVQSWP 132
           +SAHYAGED+V K+ AG++WKKVFGPVF YLN   D + D L LW+DAK Q + EVQSWP
Sbjct: 263 LSAHYAGEDMVMKVKAGDSWKKVFGPVFTYLNCLPDKTSDPLSLWQDAKNQMLTEVQSWP 322

Query: 133 YSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQ 192
           Y FPAS+DFP S++R  ISG+LLV D ++++D +PA+GAFVGLAPPG+VGSWQ ESKGYQ
Sbjct: 323 YDFPASEDFPVSDKRGCISGRLLVCDKFLSDDFLPANGAFVGLAPPGEVGSWQLESKGYQ 382

Query: 193 FWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQ 252
           FW+ AD  G F+I+ +R G+YNL  +V G+IGDY+Y+ LIN+T G ++D+G+I YEPPR 
Sbjct: 383 FWTEADSDGYFAINDIREGEYNLNGYVTGWIGDYQYEQLINITAGCDIDVGNIVYEPPRD 442

Query: 253 GATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTV 312
           G T+WEIGIPDRSAAEF+VPDPNP Y+NKLY+ HPD+FRQYGLWERY+EL+P  DLV+T+
Sbjct: 443 GPTVWEIGIPDRSAAEFFVPDPNPKYINKLYIGHPDRFRQYGLWERYTELYPKEDLVFTI 502

Query: 313 GVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVSLAELQVG 372
           GVSDY KDWFFA V RKM + TY+ TTWQ+KF+L++V    TYK+R+ALA+ ++AELQV 
Sbjct: 503 GVSDYKKDWFFAHVTRKMGDDTYQKTTWQIKFKLENVQKSCTYKIRIALATANVAELQVR 562

Query: 373 VNE---AKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQP-RC 432
           +N+    K   +F++G+IG DN+IARHGIHG+Y +YN+D+P  +LV G+NT+F TQ    
Sbjct: 563 MNDDDTEKTTPIFTTGVIGHDNAIARHGIHGIYRLYNVDVPSEKLVEGDNTLFLTQTMTT 622

Query: 433 TGPFQAIMYDYIRLEAPPPPSSAH 452
           TG F  +MYDYIRLE PP  S +H
Sbjct: 623 TGAFNGLMYDYIRLEGPPLDSYSH 646

[Close]

BLAST of Cucsat.G4655.T4 vs. TAIR 10
Match: AT1G09880.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 615.9 bits (1587), Expect = 2.6e-176
Identity = 278/435 (63.91%), Postives = 344/435 (79.08%), Query Frame = 0


Query: 13  KVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVGPTTLAVF 72
           +VDDKY+YS ++KD  VHGWIST+ +VGFW I+PSNEFRS GP+KQ L SHVGPT LAVF
Sbjct: 188 EVDDKYEYSMESKDIKVHGWISTNDSVGFWQITPSNEFRSAGPLKQFLGSHVGPTNLAVF 247

Query: 73  ISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVIEVQSWPY 132
            S HY G DL+     GEAWKKVFGPVFIYLNS     D L LW +AK Q  IE + WPY
Sbjct: 248 HSTHYVGADLIMSFKNGEAWKKVFGPVFIYLNSFPKGVDPLLLWHEAKNQTKIEEEKWPY 307

Query: 133 SFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQRESKGYQF 192
           +F ASDDFP S+QR S+SG+LLV+D +++ + IPA+G++VGLA PGDVGSWQRE KGYQF
Sbjct: 308 NFTASDDFPASDQRGSVSGRLLVRDRFISSEDIPANGSYVGLAAPGDVGSWQRECKGYQF 367

Query: 193 WSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDITYEPPRQG 252
           WS+ADE+G+FSI+ VR G YNLYA+ PGFIGDY  D + +++ GS++ +GD+ YEPPR G
Sbjct: 368 WSKADENGSFSINNVRSGRYNLYAFAPGFIGDYHNDTVFDISPGSKISLGDLVYEPPRDG 427

Query: 253 ATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELFPDSDLVYTVG 312
           +TLWEIG+PDRSAAEFY+PDPNP++VNKLYLNH DK+RQYGLWERYSEL+PD D+VY V 
Sbjct: 428 STLWEIGVPDRSAAEFYIPDPNPSFVNKLYLNHSDKYRQYGLWERYSELYPDEDMVYNVD 487

Query: 313 VSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQAT--YKLRVALASVSLAELQV 372
           + DY+K+WFF QV RK  NG YKGTTWQ++F+ DD     T  +KLR+ALA+ ++AELQV
Sbjct: 488 IDDYSKNWFFMQVTRKQANGGYKGTTWQIRFQFDDKMKNVTGNFKLRIALATSNVAELQV 547

Query: 373 GVNEAKA-KAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQPRCTG 432
            VN+  A   +F +  IGRDN+IARHGIHGLYW+Y++++P   L  GNNTI+ TQ   T 
Sbjct: 548 RVNDLSADPPLFRTEQIGRDNTIARHGIHGLYWLYSVNVPAASLHVGNNTIYLTQALATS 607

Query: 433 PFQAIMYDYIRLEAP 445
           PFQ +MYDYIRLE P
Sbjct: 608 PFQGLMYDYIRLEYP 622

[Close]

BLAST of Cucsat.G4655.T4 vs. TAIR 10
Match: AT2G22620.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 573.2 bits (1476), Expect = 1.9e-163
Identity = 258/440 (58.64%), Postives = 332/440 (75.45%), Query Frame = 0


Query: 6   SNFIWTVKVDDKYQYSCDNKDSGVHGWISTDPAVGFWLISPSNEFRSGGPVKQNLTSHVG 65
           SN ++  +VDDKY YS ++KD+ VHGWIS+DP VGFW+I+PS+EFR GGP+KQ+LTSH G
Sbjct: 235 SNPMFKGEVDDKYMYSMEDKDNNVHGWISSDPPVGFWMITPSDEFRLGGPIKQDLTSHAG 294

Query: 66  PTTLAVFISAHYAGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSFDALQLWEDAKAQAVI 125
           P TL++F S HYAG+++      GE WKKVFGPV  YLNS       L+LW DAK Q   
Sbjct: 295 PITLSMFTSTHYAGKEMRMDYRNGEPWKKVFGPVLAYLNSVSPKDSTLRLWRDAKRQMAA 354

Query: 126 EVQSWPYSFPASDDFPRSNQRSSISGKLLVKDSYVNEDLIPADGAFVGLAPPGDVGSWQR 185
           EV+SWPY F  S+D+P  +QR ++ G+ L+KDSYV+   I    AFVGLAP G+ GSWQ 
Sbjct: 355 EVKSWPYDFITSEDYPLRHQRGTLEGQFLIKDSYVSRLKIYGKFAFVGLAPIGEAGSWQT 414

Query: 186 ESKGYQFWSRADESGNFSIDGVRPGDYNLYAWVPGFIGDYRYDALINLTEGSEVDMGDIT 245
           ESKGYQFW++AD  G F I+ VR G+Y+LYAW  GFIGDY+Y+  I +T GSE+++G + 
Sbjct: 415 ESKGYQFWTKADRRGRFIIENVRAGNYSLYAWGSGFIGDYKYEQNITITPGSEMNVGPLV 474

Query: 246 YEPPRQGATLWEIGIPDRSAAEFYVPDPNPNYVNKLYLNH-PDKFRQYGLWERYSELFPD 305
           YEPPR G TLWEIG+PDR+A EFY+PDP P  +NKLY+N   D+FRQYGLW+RY++L+P 
Sbjct: 475 YEPPRNGPTLWEIGVPDRTAGEFYIPDPYPTLMNKLYVNPLQDRFRQYGLWDRYADLYPQ 534

Query: 306 SDLVYTVGVSDYTKDWFFAQVPRKMENGTYKGTTWQVKFELDDVDNQATYKLRVALASVS 365
           +DLVYT+GVSDY  DWFFA V R + N TY+ TTWQ+ F L +V+    Y LR+ALAS +
Sbjct: 535 NDLVYTIGVSDYRSDWFFAHVARNVGNDTYQPTTWQIIFNLKNVNRIGRYTLRIALASAA 594

Query: 366 LAELQVGVNEAKAKAVFSSGLIGRDNSIARHGIHGLYWVYNIDIPGNRLVTGNNTIFFTQ 425
            +ELQ+ +N+ K+ A+F++G IG+DN+IARHGIHGLY +Y+ID+ GN L  G+NTIF TQ
Sbjct: 595 DSELQIRINDPKSDAIFTTGFIGKDNAIARHGIHGLYRLYSIDVAGNLLSVGDNTIFLTQ 654

Query: 426 PRCTGPFQAIMYDYIRLEAP 445
            R   PFQ IMYDYIRLE+P
Sbjct: 655 TRSRTPFQGIMYDYIRLESP 674

The following BLAST results are available for this feature:

BLAST of Cucsat.G4655.T4 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A5ABH4	2.6e-24	26.22	Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / F...	[more]
Q5AZ85	2.5e-19	24.16	Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 ...	[more]
Q2U5P7	7.1e-14	24.46	Probable rhamnogalacturonate lyase C OS=Aspergillus oryzae (strain ATCC 42149 / ...	[more]

BLAST of Cucsat.G4655.T4 vs. NCBI nr


Match Name	Identity	Description
XP_031736466.1	99.77	probable rhamnogalacturonate lyase B isoform X2 [Cucumis sativus]	[more]
XP_004152608.2	99.77	probable rhamnogalacturonate lyase B isoform X1 [Cucumis sativus] >KGN62737.1 hy...	[more]
XP_008444895.1	95.95	PREDICTED: probable rhamnogalacturonate lyase B isoform X1 [Cucumis melo]	[more]
XP_008444896.1	95.95	PREDICTED: probable rhamnogalacturonate lyase B isoform X2 [Cucumis melo]	[more]
KAA0065109.1	95.72	putative rhamnogalacturonate lyase B isoform X1 [Cucumis melo var. makuwa]	[more]

BLAST of Cucsat.G4655.T4 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPL0	0.0	99.77	Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_2G370370 PE=3 SV=...	[more]
A0A1S3BCA7	0.0	95.95	Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103488110 PE=3 SV=1	[more]
A0A1S3BAY7	0.0	95.95	Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103488110 PE=3 SV=1	[more]
A0A5A7VI09	0.0	95.72	Rhamnogalacturonan endolyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]
A0A6J1GGJ8	6.07e-300	86.85	Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111454003 PE=3 ...	[more]

BLAST of Cucsat.G4655.T4 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09890.1	1.4e-190	67.97	Rhamnogalacturonate lyase family protein	[more]
AT1G09910.1	6.7e-185	65.67	Rhamnogalacturonate lyase family protein	[more]
AT4G24430.1	1.8e-177	63.06	Rhamnogalacturonate lyase family protein	[more]
AT1G09880.1	2.6e-176	63.91	Rhamnogalacturonate lyase family protein	[more]
AT2G22620.1	1.9e-163	58.64	Rhamnogalacturonate lyase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.60.40.1120		coord: 144..240 e-value: 5.0E-8 score: 35.1
None	No IPR available	GENE3D	2.60.120.260		coord: 251..442 e-value: 2.4E-20 score: 74.8
None	No IPR available	PANTHER	PTHR32018	RHAMNOGALACTURONATE LYASE FAMILY PROTEIN	coord: 11..445
None	No IPR available	PANTHER	PTHR32018:SF8	RHAMNOGALACTURONATE LYASE FAMILY PROTEIN	coord: 11..445
None	No IPR available	CDD	cd10317	RGL4_C	coord: 256..442 e-value: 5.99119E-54 score: 175.927
IPR029413	Rhamnogalacturonan lyase, domain II	PFAM	PF14686	fn3_3	coord: 168..239 e-value: 2.9E-24 score: 84.7
IPR029413	Rhamnogalacturonan lyase, domain II	CDD	cd10316	RGL4_M	coord: 145..244 e-value: 1.68617E-30 score: 111.572
IPR029411	Rhamnogalacturonan lyase, domain III	PFAM	PF14683	CBM-like	coord: 254..441 e-value: 2.3E-57 score: 193.6
IPR014718	Glycoside hydrolase-type carbohydrate-binding	GENE3D	2.70.98.10		coord: 6..104 e-value: 1.3E-6 score: 30.0
IPR013784	Carbohydrate-binding-like fold	SUPERFAMILY	49452	Starch-binding domain-like	coord: 168..240
IPR008979	Galactose-binding-like domain superfamily	SUPERFAMILY	49785	Galactose-binding domain-like	coord: 252..442

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G4655	Cucsat.G4655	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G4655.T4.E1	Cucsat.G4655.T4.E1	exon
Cucsat.G4655.T4.E2	Cucsat.G4655.T4.E2	exon
Cucsat.G4655.T4.E3	Cucsat.G4655.T4.E3	exon
Cucsat.G4655.T4.E4	Cucsat.G4655.T4.E4	exon
Cucsat.G4655.T4.E5	Cucsat.G4655.T4.E5	exon
Cucsat.G4655.T4.E6	Cucsat.G4655.T4.E6	exon
Cucsat.G4655.T4.E7	Cucsat.G4655.T4.E7	exon
Cucsat.G4655.T4.E8	Cucsat.G4655.T4.E8	exon
Cucsat.G4655.T4.E9	Cucsat.G4655.T4.E9	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G4655.T4.C1	Cucsat.G4655.T4.C1	CDS
Cucsat.G4655.T4.C2	Cucsat.G4655.T4.C2	CDS
Cucsat.G4655.T4.C3	Cucsat.G4655.T4.C3	CDS
Cucsat.G4655.T4.C4	Cucsat.G4655.T4.C4	CDS
Cucsat.G4655.T4.C5	Cucsat.G4655.T4.C5	CDS
Cucsat.G4655.T4.C6	Cucsat.G4655.T4.C6	CDS
Cucsat.G4655.T4.C7	Cucsat.G4655.T4.C7	CDS
Cucsat.G4655.T4.C8	Cucsat.G4655.T4.C8	CDS
Cucsat.G4655.T4.C9	Cucsat.G4655.T4.C9	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G4655.T4	Cucsat.G4655.T4-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0102210	rhamnogalacturonan endolyase activity