Cucsat.G16774.T5 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G16774.T5
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	15-cis-zeta-carotene isomerase, chloroplastic
Location	ctg24: 930703 .. 933702 (-)
RNA-Seq Expression	Cucsat.G16774.T5
Synteny	Cucsat.G16774.T5

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGAAATTTTTTTGAAGAGCATAGTTTAACAATTGACATGACCATTTTTTGTAAGTCAATGATTGGACCCATTGCCGAAATTGTTGTAGTGAAAGAAACGTTTCTCCCAAGTAATTTGCATATAGAGAATTGGTATCACTTATGAGAGTAGCGTAGGCCTGCAATTTGCATATACATAATTCCATAAAATCCAAAACTTCCTTTGAAGAGCACCAATTACCTCAGATTCTCTCTTCCATTTTCCATTCCTCGATTCCTGATTGCCTTCAACCCTCACTTCCCACTCACAACTACCTCACTATTCAGTGCTCCAATGGCGGTTGCCTCCGCCACTCCCCCTTTTTCCCCTCCATTTCTCTCCTCCCCTCGCCATCGCCATCGCCGTCCTGATGCAATTTCCTTCCGACCCTTTTCAATTTCGACCCCCAATTGCCTTCAGCAACTTCCCCTTTCTCTCAATTCCTTCCACATTTTCCGACGTCCTCGATTGGTCGCCGAGGCTTCTATTGGAGACAGGGAGAGTGGTGGGTCGACCTCTGTCTCCGACGACGAGGGCTTTGTCGGCGAGGACGCTGCTGCTTTTGATCTTTCCGAGCAGAAATTGACTTCGTGGGTTTATTTCACTGTGATTTTGGGGGTTGTTCTGTTCGTGTTGAATGTTGTTTGGATTGATAACTCTGCTGGGGGTGTTGGGAAGGCCTTTCTGGATGCTGTTTCTGGAATTTCGGATAGTCATGAGGTTCACTTTTTTTGTGTTACTTCGTTTTGTTTAATTTGATTCACATTTGGAGATGAAGCCTGTATAGAAATAGTTCCATTGCCATAGAAGCAGTGTTCATAGTAATTCTGCTGTTTTATAGTTTATATATAGTGTTTCTCTCAGATGGTTTGTACGTGTTGGGTTCGTTGCTAAGACTTTTAGAAAGTTCAATTTTGTCCTTAATTTTAGTAAACTTCTAATGAATATGTAGAAGTTCTAAAATTGTATTCATTTGTAGCATTTTTGGGCTGTCTTGTGATATAGGAATATTTCCAATTCAGTTAGTAAAATATGTACATAACTCACAGATTTTGATAGAAAAATCAATCTTGATACATAAAGGATTGTATTGAAACTACGATCTTATTTGAACGAGATCTGTTTTGTAGGTTGTATATCTCTGTCCTTGAGTTCTAAAGGATTGTATTGAAACTGATTAAGGTCTTGTATGTCTGCTGTATATGAATGATTGGGGTGGATGAACATGGCATAAGGATTATATTGCTCATGGTTTAATGTTGAATTTTGGAAACTCACTTGATTATTTGATTATGAATCTAAGTCTTGAAACTTTCTTCCGTAGGTTGTGATGTTGCTCCTCATATTCATTTTTGCCATTGTCCATAGTGGTTTGGCCAGTCTCCGAGATCAGGGCGAGAAGCTTGTCGGGGAACGAGCTTTTCGGGTTTTGTTTGCTGGAGTTTCTCTGCCATTGGCTGTTAGCACTGTGGTAGGTACCTTACCGCTTCTTTTCCCTTGAACTTGATGCAGTAAATGCAATGTTCTTGAAGTTACCTCAGTACCTTTTATTCATTCAAGTTTTCCGAATGCACACGCATATTGACTTGTTTACACTAAGGAATTATATTCAGGTGTATTTCATTAACCATCGATACGATGGAGTACAGTTATGGCAGCTCCAAAGTGTTCCCCTACTGCATCAACTTGTGTGGCTTAGCTCGTTTGTCTCCTTCATCTTTCTCTATCCTTCAACTTTTAATCTGCTGGAGGTTGCAGCAGTTGATAAACCAAAAATGCACCTTTGGGAAACAGGCATCATTAGAATAACTAGACATCCACAGGTTTGTTCGTGTTTCATTATCTTCAATGTTCATTTCATTTCATTATAATATCCGGGTTCTATTGTTGAGTAGTACTTGGGTGCAGTCTAGGTTGCACCATGTCCATGAGATGACTGTCAATTAAAAATGAAAAAAAATAGAACATAAGACAATCGTTTTTAAAAAGTAAATTTGCTACATGGCAGCTTGTGATTTGCACTTGGGTGGGTTGCACAAGATAGTTGCATCCTAGGATGCTCCCAACTATTTTTCTTTATTAGTGCTAGTTTGCTGGCTTCGACTTGAATTCATCGATGAACTTGAGTTTTAATTTTGTGTACCACTTGAAATACACGTCTCATAGACAAATTTCTTATGTCAAGTGTCCTCAAATAGTAGGGTTGAACAATAAATTAGCCAAATGAAAGCTTAGAAACCATAATATAGGATTTAAAACCAAACTGTATATTGCATTAGACGAGTTTTTTCAGAAGTCACATTTGGTCTTCTAGAATAACACGAGAGAAGATGAAATCCAATTTCGTCCCTCTCTGTTTCCTGATAAAATGTTGATACTTCATGAGGGGAGGTTAAAAAAGACCACCAACCATGAAAAGTCAAACTGACCTGGCAAATCTTGGTTTTCTTTTAAGATGGTTGGACAGGTGATATGGTGTCTTGCTCATACAATCTGGATTGGGAACTCTGTTGCAGTAGCAGCTTCCATTGGTTTGATAGGACATCATCTGTTTGGCGTATGGAACGGAGACAGGAGGCTAGCCAAGCGATATGGGGCAGATTTTGAAGCCGTGAAAAGCCGAACAAGCATCATCCCATTCGCTGCCATTGTGGATGGTCGTCAAAAGTTGCCCGACGATTACTACAAGGAGTTCCTTCGCTTGCCATATCTATCAATCACTGCACTAACAATAGGAGCTTACCTTGCTCACCCTCTTATGCAAGCTGCTAGTTTCAGGCTTCATTGGTAACTCCATTTCTATGCAGCTAACTCTCCTGTTGTATCATAAATACTCAAATGTATTTCATTTGTTGTATAAACCAAAGCATAGAGCATACAGAAATGTGAGGCTATAGTCATAGCCTTTTTTATTACAAAGTTATGAAATAAAAATCATTCAACCTTATAAAACTATAAAAATGCCCTTCAAGAAAAATTAT

Coding sequence (CDS)

ATGGCGGTTGCCTCCGCCACTCCCCCTTTTTCCCCTCCATTTCTCTCCTCCCCTCGCCATCGCCATCGCCGTCCTGATGCAATTTCCTTCCGACCCTTTTCAATTTCGACCCCCAATTGCCTTCAGCAACTTCCCCTTTCTCTCAATTCCTTCCACATTTTCCGACGTCCTCGATTGGTCGCCGAGGCTTCTATTGGAGACAGGGAGAGTGGTGGGTCGACCTCTGTCTCCGACGACGAGGGCTTTGTCGGCGAGGACGCTGCTGCTTTTGATCTTTCCGAGCAGAAATTGACTTCGTGGGTTTATTTCACTGTGATTTTGGGGGTTGTTCTGTTCGTGTTGAATGTTGTTTGGATTGATAACTCTGCTGGGGGTGTTGGGAAGGCCTTTCTGGATGCTGTTTCTGGAATTTCGGATAGTCATGAGGTTGTGATGTTGCTCCTCATATTCATTTTTGCCATTGTCCATAGTGGTTTGGCCAGTCTCCGAGATCAGGGCGAGAAGCTTGTCGGGGAACGAGCTTTTCGGGTTTTGTTTGCTGGAGTTTCTCTGCCATTGGCTGTTAGCACTGTGGTGTATTTCATTAACCATCGATACGATGGAGTACAGTTATGGCAGCTCCAAAGTGTTCCCCTACTGCATCAACTTGTGTGGCTTAGCTCGTTTGTCTCCTTCATCTTTCTCTATCCTTCAACTTTTAATCTGCTGGAGGTTGCAGCAGTTGATAAACCAAAAATGCACCTTTGGGAAACAGGCATCATTAGAATAACTAGACATCCACAGGTTTGTTCGTGTTTCATTATCTTCAATGTTCATTTCATTTCATTATAA

Protein sequence

MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLVAEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWIDNSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFAGVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAAVDKPKMHLWETGIIRITRHPQVCSCFIIFNVHFISL

Homology

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BLAST of Cucsat.G16774.T5 vs. ExPASy Swiss-Prot
Match: Q9SAC0 (15-cis-zeta-carotene isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=Z-ISO PE=1 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 3.2e-78
Identity = 157/252 (62.30%), Postives = 190/252 (75.40%), Query Frame = 0


Query: 11  SPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLVAEASIGDRES 70
           SPP L       RRP+    R    + P       L  +S  + R  +++  +++ + + 
Sbjct: 10  SPPSLLLLPPSPRRPNLTLIRRIP-AHPRLGNSTSLLSSSSPVIR--KILVRSTLREDQP 69

Query: 71  GGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWIDNSAGGVGKAF 130
             S S S     +GED+AAF+L +QKL SWVYF V+LGVVLF+LNVVWIDNS  G GK+F
Sbjct: 70  IASDSESSPTLLIGEDSAAFELGKQKLVSWVYFGVVLGVVLFILNVVWIDNST-GFGKSF 129

Query: 131 LDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFAGVSLPLAVST 190
           +DAVS IS S EV ML+LI IFAIVHSGLASLRD GEKL+GERAFRVLFAG+SLPLA+ST
Sbjct: 130 IDAVSNISGSPEVAMLMLILIFAIVHSGLASLRDIGEKLIGERAFRVLFAGISLPLAMST 189

Query: 191 VVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAAVDKPKMHLWE 250
           +VYFINHRYDG QLWQLQ VP +H+ +W+++FVSF FLYPSTFNLLEVAAVDKPKMHLWE
Sbjct: 190 IVYFINHRYDGSQLWQLQGVPGVHEAIWVANFVSFFFLYPSTFNLLEVAAVDKPKMHLWE 249

Query: 251 TGIIRITRHPQV 263
           TGI+RITRHPQ+
Sbjct: 250 TGIMRITRHPQM 257

[Close]

BLAST of Cucsat.G16774.T5 vs. ExPASy Swiss-Prot
Match: B4FHU1 (15-cis-zeta-carotene isomerase, chloroplastic OS=Zea mays OX=4577 PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.4e-73
Identity = 149/256 (58.20%), Postives = 179/256 (69.92%), Query Frame = 0


Query: 11  SPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLVAEASIGDRES 70
           +PP L   R    RP   +  P     P      PLS    H   RP       I  +E 
Sbjct: 12  TPPLLPHRRPHLARPLCPTLNPIRAPLP------PLSRVLSH--ARPARAVGGGIEPKE- 71

Query: 71  GGSTSVSDDEG----FVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWIDNSAGGV 130
            G  +  D+ G     VGED+AAF+L +Q + SW YF  ILG VL  LNV+WID S  GV
Sbjct: 72  -GVVAEGDESGGGPVLVGEDSAAFELKDQSVASWAYFAGILGAVLVALNVLWIDPST-GV 131

Query: 131 GKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFAGVSLPL 190
           G  FLDAV+ +SDSHEVVMLLL  IFA+VHSG+ASLR+ GEK+VGER +RVLFAG+SLPL
Sbjct: 132 GTKFLDAVASVSDSHEVVMLLLTIIFAVVHSGMASLRESGEKIVGERVYRVLFAGISLPL 191

Query: 191 AVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAAVDKPKM 250
           AV+T+VYFINHRYDG QLWQ+Q +  +H+L+W SSF+SF FLYPSTFNLLEVAAVDKPK+
Sbjct: 192 AVTTIVYFINHRYDGTQLWQVQGITGIHELLWFSSFISFFFLYPSTFNLLEVAAVDKPKL 251

Query: 251 HLWETGIIRITRHPQV 263
           H+WETGI+RITRHPQ+
Sbjct: 252 HMWETGIMRITRHPQM 256

[Close]

BLAST of Cucsat.G16774.T5 vs. NCBI nr
Match: XP_004152101.1 (15-cis-zeta-carotene isomerase, chloroplastic isoform X1 [Cucumis sativus] >KGN53082.1 hypothetical protein Csa_015404 [Cucumis sativus])

HSP 1 Score: 509 bits (1312), Expect = 7.33e-180
Identity = 261/262 (99.62%), Postives = 262/262 (100.00%), Query Frame = 0


Query: 1   MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV 60
           MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV
Sbjct: 1   MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV 60

Query: 61  AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120
           AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID
Sbjct: 61  AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120

Query: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180
           NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA
Sbjct: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180

Query: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA 240
           GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA
Sbjct: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA 240

Query: 241 VDKPKMHLWETGIIRITRHPQV 262
           VDKPKMHLWETGIIRITRHPQ+
Sbjct: 241 VDKPKMHLWETGIIRITRHPQM 262

[Close]

BLAST of Cucsat.G16774.T5 vs. NCBI nr
Match: TYK16983.1 (15-cis-zeta-carotene isomerase [Cucumis melo var. makuwa])

HSP 1 Score: 474 bits (1221), Expect = 1.33e-167
Identity = 247/262 (94.27%), Postives = 248/262 (94.66%), Query Frame = 0


Query: 1   MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV 60
           MAVASATPPFSPP+LSSPRHR  RPDAIS RPFSIS PNCL QLPLSLNS HIFRRPR  
Sbjct: 1   MAVASATPPFSPPYLSSPRHR--RPDAISLRPFSISAPNCLLQLPLSLNSLHIFRRPRFA 60

Query: 61  AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120
           AEASIGDRESGGSTSVSDDEG VGEDAA FDLSEQKLTSWVYFTVILGVVLFVLNVVWID
Sbjct: 61  AEASIGDRESGGSTSVSDDEGLVGEDAAVFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120

Query: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180
           NSAGGVGKAFLDAVSGISDSHEVVMLLL  IFAIVHSGLASLRDQGEKLVGERAFRVLFA
Sbjct: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLTLIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180

Query: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA 240
           GVSLPLAVSTVVYFINHRYDGVQLWQLQSVP LHQLVWLSSFVSF FLYPSTFNLLEVAA
Sbjct: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPGLHQLVWLSSFVSFFFLYPSTFNLLEVAA 240

Query: 241 VDKPKMHLWETGIIRITRHPQV 262
           VDKPKMHLWETGIIRITRHPQV
Sbjct: 241 VDKPKMHLWETGIIRITRHPQV 260

[Close]

BLAST of Cucsat.G16774.T5 vs. NCBI nr
Match: KAA0044603.1 (15-cis-zeta-carotene isomerase [Cucumis melo var. makuwa])

HSP 1 Score: 477 bits (1227), Expect = 5.13e-167
Identity = 248/265 (93.58%), Postives = 249/265 (93.96%), Query Frame = 0


Query: 1   MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV 60
           MAVASATPPFSPP+LSSPRHR  RPDAIS RPFSIS PNCL QLPLSLNS HIFRRPR  
Sbjct: 1   MAVASATPPFSPPYLSSPRHR--RPDAISLRPFSISAPNCLLQLPLSLNSLHIFRRPRFA 60

Query: 61  AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120
           AEASIGDRESGGSTSVSDDEG VGEDAA FDLSEQKLTSWVYFTVILGVVLFVLNVVWID
Sbjct: 61  AEASIGDRESGGSTSVSDDEGLVGEDAAVFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120

Query: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180
           NSAGGVGKAFLDAVSGISDSHEVVMLLL  IFAIVHSGLASLRDQGEKLVGERAFRVLFA
Sbjct: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLTLIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180

Query: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA 240
           GVSLPLAVSTVVYFINHRYDGVQLWQLQSVP LHQLVWLSSFVSF FLYPSTFNLLEVAA
Sbjct: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPGLHQLVWLSSFVSFFFLYPSTFNLLEVAA 240

Query: 241 VDKPKMHLWETGIIRITRHPQVCSC 265
           VDKPKMHLWETGIIRITRHPQV  C
Sbjct: 241 VDKPKMHLWETGIIRITRHPQVIWC 263

[Close]

BLAST of Cucsat.G16774.T5 vs. NCBI nr
Match: XP_031740105.1 (15-cis-zeta-carotene isomerase, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 474 bits (1221), Expect = 3.40e-166
Identity = 249/262 (95.04%), Postives = 250/262 (95.42%), Query Frame = 0


Query: 1   MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV 60
           MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV
Sbjct: 1   MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV 60

Query: 61  AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120
           AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID
Sbjct: 61  AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120

Query: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180
           NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA
Sbjct: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180

Query: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA 240
           GVSLPLAVSTV            LWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA
Sbjct: 181 GVSLPLAVSTV------------LWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA 240

Query: 241 VDKPKMHLWETGIIRITRHPQV 262
           VDKPKMHLWETGIIRITRHPQ+
Sbjct: 241 VDKPKMHLWETGIIRITRHPQM 250

[Close]

BLAST of Cucsat.G16774.T5 vs. NCBI nr
Match: XP_008453974.1 (PREDICTED: 15-cis-zeta-carotene isomerase, chloroplastic [Cucumis melo])

HSP 1 Score: 473 bits (1218), Expect = 1.39e-165
Identity = 246/262 (93.89%), Postives = 248/262 (94.66%), Query Frame = 0


Query: 1   MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV 60
           MAVASATPPFSPP+LSSPRHR  RPDAIS RPFSIS PNCL QLPLSLNS HIFRRPR  
Sbjct: 1   MAVASATPPFSPPYLSSPRHR--RPDAISLRPFSISAPNCLLQLPLSLNSLHIFRRPRFA 60

Query: 61  AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120
           AEASIGDRESGGSTSVSDDEG VGEDAA FDLSEQKLTSWVYFTVILGVVLFVLNVVWID
Sbjct: 61  AEASIGDRESGGSTSVSDDEGLVGEDAAVFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120

Query: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180
           NSAGGVGKAFLDAVSGISDSHEVVMLLL  IFAIVHSGLASLRDQGEKLVGERAFRVLFA
Sbjct: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLTLIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180

Query: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA 240
           GVSLPLAVSTVVYFINHRYDGVQLWQLQSVP LHQLVWLSSFVSF FLYPSTFNLLEVAA
Sbjct: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPGLHQLVWLSSFVSFFFLYPSTFNLLEVAA 240

Query: 241 VDKPKMHLWETGIIRITRHPQV 262
           VDKPKMHLWETGIIRITRHPQ+
Sbjct: 241 VDKPKMHLWETGIIRITRHPQM 260

[Close]

BLAST of Cucsat.G16774.T5 vs. ExPASy TrEMBL
Match: A0A0A0KXF1 (NnrU domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G015720 PE=4 SV=1)

HSP 1 Score: 509 bits (1312), Expect = 3.55e-180
Identity = 261/262 (99.62%), Postives = 262/262 (100.00%), Query Frame = 0


Query: 1   MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV 60
           MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV
Sbjct: 1   MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV 60

Query: 61  AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120
           AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID
Sbjct: 61  AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120

Query: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180
           NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA
Sbjct: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180

Query: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA 240
           GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA
Sbjct: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA 240

Query: 241 VDKPKMHLWETGIIRITRHPQV 262
           VDKPKMHLWETGIIRITRHPQ+
Sbjct: 241 VDKPKMHLWETGIIRITRHPQM 262

[Close]

BLAST of Cucsat.G16774.T5 vs. ExPASy TrEMBL
Match: A0A5D3CYJ4 (15-cis-zeta-carotene isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001240 PE=4 SV=1)

HSP 1 Score: 474 bits (1221), Expect = 6.44e-168
Identity = 247/262 (94.27%), Postives = 248/262 (94.66%), Query Frame = 0


Query: 1   MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV 60
           MAVASATPPFSPP+LSSPRHR  RPDAIS RPFSIS PNCL QLPLSLNS HIFRRPR  
Sbjct: 1   MAVASATPPFSPPYLSSPRHR--RPDAISLRPFSISAPNCLLQLPLSLNSLHIFRRPRFA 60

Query: 61  AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120
           AEASIGDRESGGSTSVSDDEG VGEDAA FDLSEQKLTSWVYFTVILGVVLFVLNVVWID
Sbjct: 61  AEASIGDRESGGSTSVSDDEGLVGEDAAVFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120

Query: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180
           NSAGGVGKAFLDAVSGISDSHEVVMLLL  IFAIVHSGLASLRDQGEKLVGERAFRVLFA
Sbjct: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLTLIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180

Query: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA 240
           GVSLPLAVSTVVYFINHRYDGVQLWQLQSVP LHQLVWLSSFVSF FLYPSTFNLLEVAA
Sbjct: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPGLHQLVWLSSFVSFFFLYPSTFNLLEVAA 240

Query: 241 VDKPKMHLWETGIIRITRHPQV 262
           VDKPKMHLWETGIIRITRHPQV
Sbjct: 241 VDKPKMHLWETGIIRITRHPQV 260

[Close]

BLAST of Cucsat.G16774.T5 vs. ExPASy TrEMBL
Match: A0A5A7TMA2 (15-cis-zeta-carotene isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003540 PE=4 SV=1)

HSP 1 Score: 477 bits (1227), Expect = 2.49e-167
Identity = 248/265 (93.58%), Postives = 249/265 (93.96%), Query Frame = 0


Query: 1   MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV 60
           MAVASATPPFSPP+LSSPRHR  RPDAIS RPFSIS PNCL QLPLSLNS HIFRRPR  
Sbjct: 1   MAVASATPPFSPPYLSSPRHR--RPDAISLRPFSISAPNCLLQLPLSLNSLHIFRRPRFA 60

Query: 61  AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120
           AEASIGDRESGGSTSVSDDEG VGEDAA FDLSEQKLTSWVYFTVILGVVLFVLNVVWID
Sbjct: 61  AEASIGDRESGGSTSVSDDEGLVGEDAAVFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120

Query: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180
           NSAGGVGKAFLDAVSGISDSHEVVMLLL  IFAIVHSGLASLRDQGEKLVGERAFRVLFA
Sbjct: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLTLIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180

Query: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA 240
           GVSLPLAVSTVVYFINHRYDGVQLWQLQSVP LHQLVWLSSFVSF FLYPSTFNLLEVAA
Sbjct: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPGLHQLVWLSSFVSFFFLYPSTFNLLEVAA 240

Query: 241 VDKPKMHLWETGIIRITRHPQVCSC 265
           VDKPKMHLWETGIIRITRHPQV  C
Sbjct: 241 VDKPKMHLWETGIIRITRHPQVIWC 263

[Close]

BLAST of Cucsat.G16774.T5 vs. ExPASy TrEMBL
Match: A0A1S3BYR9 (15-cis-zeta-carotene isomerase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494534 PE=4 SV=1)

HSP 1 Score: 473 bits (1218), Expect = 6.71e-166
Identity = 246/262 (93.89%), Postives = 248/262 (94.66%), Query Frame = 0


Query: 1   MAVASATPPFSPPFLSSPRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRPRLV 60
           MAVASATPPFSPP+LSSPRHR  RPDAIS RPFSIS PNCL QLPLSLNS HIFRRPR  
Sbjct: 1   MAVASATPPFSPPYLSSPRHR--RPDAISLRPFSISAPNCLLQLPLSLNSLHIFRRPRFA 60

Query: 61  AEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120
           AEASIGDRESGGSTSVSDDEG VGEDAA FDLSEQKLTSWVYFTVILGVVLFVLNVVWID
Sbjct: 61  AEASIGDRESGGSTSVSDDEGLVGEDAAVFDLSEQKLTSWVYFTVILGVVLFVLNVVWID 120

Query: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180
           NSAGGVGKAFLDAVSGISDSHEVVMLLL  IFAIVHSGLASLRDQGEKLVGERAFRVLFA
Sbjct: 121 NSAGGVGKAFLDAVSGISDSHEVVMLLLTLIFAIVHSGLASLRDQGEKLVGERAFRVLFA 180

Query: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLEVAA 240
           GVSLPLAVSTVVYFINHRYDGVQLWQLQSVP LHQLVWLSSFVSF FLYPSTFNLLEVAA
Sbjct: 181 GVSLPLAVSTVVYFINHRYDGVQLWQLQSVPGLHQLVWLSSFVSFFFLYPSTFNLLEVAA 240

Query: 241 VDKPKMHLWETGIIRITRHPQV 262
           VDKPKMHLWETGIIRITRHPQ+
Sbjct: 241 VDKPKMHLWETGIIRITRHPQM 260

[Close]

BLAST of Cucsat.G16774.T5 vs. ExPASy TrEMBL
Match: A0A6J1I3V9 (15-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470740 PE=4 SV=1)

HSP 1 Score: 423 bits (1088), Expect = 4.73e-146
Identity = 223/265 (84.15%), Postives = 234/265 (88.30%), Query Frame = 0


Query: 1   MAVASATPPFSPPFLSS---PRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRP 60
           MAVASAT  F  PF +S   P HRHRRPDA + RP SISTPN L QL  SLNS  I RR 
Sbjct: 1   MAVASATTLFFSPFPTSSNQPHHRHRRPDAFTLRPSSISTPNFLPQLSFSLNSSPIPRRR 60

Query: 61  RLVAEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVV 120
           R  AEASIGD ESGGSTS SDD+G VGEDAA F+LSEQKL SWVYFTVILGVVLFVLNVV
Sbjct: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWVYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAF+DAVSG+SDSHEVVML LIFIFA+VHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLE 240
           LFAGVSLPLAVSTVVYFINHRYDGVQLWQL S+P LHQLVWLSSF+SF FLYPSTFNLLE
Sbjct: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYPSTFNLLE 240

Query: 241 VAAVDKPKMHLWETGIIRITRHPQV 262
           VAAVDKPKMHLWETGI+RITRHPQ+
Sbjct: 241 VAAVDKPKMHLWETGIMRITRHPQM 265

The following BLAST results are available for this feature:

BLAST of Cucsat.G16774.T5 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SAC0	3.2e-78	62.30	15-cis-zeta-carotene isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
B4FHU1	1.4e-73	58.20	15-cis-zeta-carotene isomerase, chloroplastic OS=Zea mays OX=4577 PE=1 SV=1	[more]

BLAST of Cucsat.G16774.T5 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152101.1	7.33e-180	99.62	15-cis-zeta-carotene isomerase, chloroplastic isoform X1 [Cucumis sativus] >KGN5...	[more]
TYK16983.1	1.33e-167	94.27	15-cis-zeta-carotene isomerase [Cucumis melo var. makuwa]	[more]
KAA0044603.1	5.13e-167	93.58	15-cis-zeta-carotene isomerase [Cucumis melo var. makuwa]	[more]
XP_031740105.1	3.40e-166	95.04	15-cis-zeta-carotene isomerase, chloroplastic isoform X2 [Cucumis sativus]	[more]
XP_008453974.1	1.39e-165	93.89	PREDICTED: 15-cis-zeta-carotene isomerase, chloroplastic [Cucumis melo]	[more]

BLAST of Cucsat.G16774.T5 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KXF1	3.55e-180	99.62	NnrU domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G015720 PE=4 S...	[more]
A0A5D3CYJ4	6.44e-168	94.27	15-cis-zeta-carotene isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A5A7TMA2	2.49e-167	93.58	15-cis-zeta-carotene isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A1S3BYR9	6.71e-166	93.89	15-cis-zeta-carotene isomerase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1I3V9	4.73e-146	84.15	15-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009915	NnrU domain	PFAM	PF07298	NnrU	coord: 144..266 e-value: 2.0E-27 score: 96.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR35988	15-CIS-ZETA-CAROTENE ISOMERASE, CHLOROPLASTIC	coord: 55..272

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G16774	Cucsat.G16774	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G16774.T5.E1	Cucsat.G16774.T5.E1	exon
Cucsat.G16774.T5.E2	Cucsat.G16774.T5.E2	exon
Cucsat.G16774.T5.E3	Cucsat.G16774.T5.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G16774.T5.C3	Cucsat.G16774.T5.C3	CDS
Cucsat.G16774.T5.C2	Cucsat.G16774.T5.C2	CDS
Cucsat.G16774.T5.C1	Cucsat.G16774.T5.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G16774.T5	Cucsat.G16774.T5-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016120	carotene biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0090471	9,15,9'-tri-cis-zeta-carotene isomerase activity
molecular_function	GO:0016853	isomerase activity