Cucsat.G14820.T4 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G14820.T4
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Pentatricopeptide repeat
Location	ctg1869: 5404335 .. 5406631 (+)
RNA-Seq Expression	Cucsat.G14820.T4
Synteny	Cucsat.G14820.T4

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AATAAAATCAAGGAGTGGTTTGATTTGGGAAAAAATATATAAGGAGCAGAGTCTTTGAATGAGACTTTGCAAGGAGCAGAGTCGAGTTGAGTCTGAGAGGAAATGAAGAGAATACGGCAACAGCTTCTTACCCTACAACGTCTTCCACTCTCACCAGAGCTCCTCCGCTTTCAAATTCCCGCCATTCTTTCTCCATTTTCTTCTTCTTCCTCCTTCATTTCCGATTCTCCATCAGCTTCCATAGCCACCAAACCCAATACCACTCTGACCCACGACGAGCTCACAAGGATCAATCTCCTTCTTCCTCGTCTCTGTCTCCACAACCACCTTTCTACTGCTATCTCTCTTCTCCACGCCACTCTCCTCACCAATCCTTCACTTCACTCTCTTTCCCTTTCAGTTCTCTCCCACTCACTTGCTTCCCAGTCCGATTTTGCCCTCACAATGTCCCTCCTCACTCGTCTTAAGCACCATCCCAATGCCCTTCTCTATTCAACCCCTATCGTCACTATGCTTATTTCCTCTTATTGCAAACGCCGGAAATCCAAGGAGGCGTTGAAGCTTTTCCATTGGATGCTGAGACCGGGGTCGCCATGTAAGCCGGAAGAGAGGGTTTATAAAACCCTAATTGCGGGACTTTATAGGAAGGGTATGACATTTGATGCTTTGAAGGTTCTCAGGAATATGATTGATTCGAATTTGGTTCCGGATTGCGATTTGAGAAATTGGGTTTTTAGGTCTTTGCTGAAAGAAGCGATGATCCCCGAAGCCATGGAGTTTAATGATGCTTTGAATTTCGTTGGCGATCAGAATACAATTGACCATCTTCGAAGGGTGTCGGAATTGTTGAATCGTATCATCACCAATTGGATAGACTAGAGAAGATCTTCTGGTAAGCCATTTGCTCTCTGTCATTCTATTAATTTTTCACCATCTCTAGACTCTAATTTTGACGATATTTAGAATATAGCGGATGATTGAGTCAAGCAATTTGTTGATCATAAGCAGACAGACCTTACAATGCATTTATAAGGATTCAGCAACTTTATCACGGGATAGTCTACTGCATGATCTATTAAAGTGCATATAAAATTGGGAAGCTTGGTATCAATTATCTGATTGGTAATATTGAATTCTTTTTACTTCTTTGTATTGTGTTGTATAGTTAATTAGCAGAGTAGTTTTGTGACATTTGCTGCCATTTCATTTCAAAAGCTAGACCACATAAAGTAATTCCACACAAAAGGATTAGCATTTAAGCTAGTTAAACTATAAGCTAAAAATAAAGGGCAAGCTTCTAAACCATGTCATTCTCCCTCAACAAAGTGTAATTTCTGCATCCTTCTTGCAACTTGAATTGATATAATTGAAAGCTTAAAGCTTAGATAGCATGTAGCTAGAAGGAGAAGAGAAAGAAGAATAACAAAAACAGATATTATGGATTGTTTATGTTTAAGTGAGCATGGATAAGACAGGTTAAAGTTGATGGATTATGATAAATTTGATAGATAAGTAGTGACATTTTCTCTTGTTCAGCTTCAACAAATTTTCAAGTTTACAATGGAGGGAAAATATTTCATAACTCATCAACTTATGCGTATAACATGATTAGCGATTCAATAGCTTTTAAATTTTAGTTACTTTTCTTATATAGCTGGGTGCTAATCTAAGAGTTCAAAATCTTGCAGTATCAGGTTCCTTTCCTGGAGAAGGAAGAGAAACTTGGTCGTTACCGGTGTATTCAATTTCCAGGGAGAGGAAAGGCAGTGATGCAGGACCCCATAGTTTTCACAAATCTTCAAAGTAGAAAGGCTTGAGCATCAAGTTCACATCAAATGTTACAAACTGGGATAAACAGCTGCAGAAATTGAGGGTATAATGTAACATAATGGTGGTTAAATGTCCCTGCAACCCAAGTGGAGGTTAACTACATAATTTTTTCTCTGTTTAGTATCATCATTTTGGACATGGGGTTTCAATAGAATCTTCGCAAAGTTCTCTCAGTTGGTGGTGGGTACTAAGCATTACCTAATTCAAACTCGTTAAGACACAAGTAGGTGGGCGTTCCAACTTGAAGTCTCACTCGTTCGAGTTTAGGGAAGAAAAACATCAAACTCCAGTCAAAAGTTTGAATGTACTCGTCAGCAATTTTGAACTTGATAAAAAACACTGTCTTGTTTTATTTTTAATGTGAATATTGTATCGGTTAACTTCGAGACATTTTTATGTTCCGTATGAAATTTAAATTCGAATACATATTTCAATCATATTTCTAATTATTAACTTATTTCAATCTG

Coding sequence (CDS)

ATGAAGAGAATACGGCAACAGCTTCTTACCCTACAACGTCTTCCACTCTCACCAGAGCTCCTCCGCTTTCAAATTCCCGCCATTCTTTCTCCATTTTCTTCTTCTTCCTCCTTCATTTCCGATTCTCCATCAGCTTCCATAGCCACCAAACCCAATACCACTCTGACCCACGACGAGCTCACAAGGATCAATCTCCTTCTTCCTCGTCTCTGTCTCCACAACCACCTTTCTACTGCTATCTCTCTTCTCCACGCCACTCTCCTCACCAATCCTTCACTTCACTCTCTTTCCCTTTCAGTTCTCTCCCACTCACTTGCTTCCCAGTCCGATTTTGCCCTCACAATGTCCCTCCTCACTCGTCTTAAGCACCATCCCAATGCCCTTCTCTATTCAACCCCTATCGTCACTATGCTTATTTCCTCTTATTGCAAACGCCGGAAATCCAAGGAGGCGTTGAAGCTTTTCCATTGGATGCTGAGACCGGGGTCGCCATGTAAGCCGGAAGAGAGGGTTTATAAAACCCTAATTGCGGGACTTTATAGGAAGGGTATGACATTTGATGCTTTGAAGGTTCTCAGGAATATGATTGATTCGAATTTGGTTCCGGATTGCGATTTGAGAAATTGGGTTTTTAGGTCTTTGCTGAAAGAAGCGATGATCCCCGAAGCCATGGAGTTTAATGATGCTTTGAATTTCGTTGGCGATCAGAATACAATTGACCATCTTCGAAGGGTGTCGGAATTGTTGAATCGTATCATCACCAATTGGATAGACTAG

Protein sequence

MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSPSASIATKPNTTLTHDELTRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTRLKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLYRKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNTIDHLRRVSELLNRIITNWID

Homology

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BLAST of Cucsat.G14820.T4 vs. ExPASy Swiss-Prot
Match: Q9FJE6 (Putative pentatricopeptide repeat-containing protein At5g59900 OS=Arabidopsis thaliana OX=3702 GN=At5g59900 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.8e-06
Identity = 44/166 (26.51%), Postives = 74/166 (44.58%), Query Frame = 0


Query: 64  NLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALT---MSLLTR 123
           ++L+   C    L TA+S L   + T   L     + L +      D +     M+ +  
Sbjct: 406 SILIDMFCRRGKLDTALSFLGEMVDTGLKLSVYPYNSLINGHCKFGDISAAEGFMAEMIN 465

Query: 124 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY 183
            K  P  + Y     T L+  YC + K  +AL+L+H M   G    P    + TL++GL+
Sbjct: 466 KKLEPTVVTY-----TSLMGGYCSKGKINKALRLYHEM--TGKGIAPSIYTFTTLLSGLF 525

Query: 184 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEF 227
           R G+  DA+K+   M + N+ P+    N +     +E  + +A EF
Sbjct: 526 RAGLIRDAVKLFNEMAEWNVKPNRVTYNVMIEGYCEEGDMSKAFEF 564

[Close]

BLAST of Cucsat.G14820.T4 vs. ExPASy Swiss-Prot
Match: Q9FND8 (Pentatricopeptide repeat-containing protein At5g40400 OS=Arabidopsis thaliana OX=3702 GN=At5g40400 PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.4e-06
Identity = 36/133 (27.07%), Postives = 67/133 (50.38%), Query Frame = 0


Query: 94  HSLSLSVLSHSLASQSDFALTMSLLTRLKH---HPNALLYSTPIVTMLISSYCKRRKSKE 153
           ++ + ++L++   + S+F      L +++     P+ + Y+T     L+SSYC+R + KE
Sbjct: 235 NTYTFNILTNVFCNDSNFREVDDFLEKMEEEGFEPDLVTYNT-----LVSSYCRRGRLKE 294

Query: 154 ALKLFHWMLRPGSPCKPEERVYKTLIAGLYRKGMTFDALKVLRNMIDSNLVPDCDLRNWV 213
           A  L+  M R      P+   Y +LI GL + G   +A +    M+D  + PDC   N +
Sbjct: 295 AFYLYKIMYR--RRVVPDLVTYTSLIKGLCKDGRVREAHQTFHRMVDRGIKPDCMSYNTL 354

Query: 214 FRSLLKEAMIPEA 224
             +  KE M+ ++
Sbjct: 355 IYAYCKEGMMQQS 360

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BLAST of Cucsat.G14820.T4 vs. ExPASy Swiss-Prot
Match: Q3EDF8 (Pentatricopeptide repeat-containing protein At1g09900 OS=Arabidopsis thaliana OX=3702 GN=At1g09900 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.5e-05
Identity = 44/166 (26.51%), Postives = 78/166 (46.99%), Query Frame = 0


Query: 64  NLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTRLKH 123
           N+L+  LC    L  AI +L          +SLS + L H    +      +  L R+  
Sbjct: 348 NILINFLCRKGLLGRAIDILEKMPQHGCQPNSLSYNPLLHGFCKEKKMDRAIEYLERMVS 407

Query: 124 ---HPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY 183
              +P+ + Y+T     ++++ CK  K ++A+++ + +   G  C P    Y T+I GL 
Sbjct: 408 RGCYPDIVTYNT-----MLTALCKDGKVEDAVEILNQLSSKG--CSPVLITYNTVIDGLA 467

Query: 184 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEF 227
           + G T  A+K+L  M   +L PD    + +   L +E  + EA++F
Sbjct: 468 KAGKTGKAIKLLDEMRAKDLKPDTITYSSLVGGLSREGKVDEAIKF 506

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BLAST of Cucsat.G14820.T4 vs. ExPASy Swiss-Prot
Match: Q0WKZ3 (Pentatricopeptide repeat-containing protein At1g64580 OS=Arabidopsis thaliana OX=3702 GN=At1g64580 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 2.0e-05
Identity = 47/200 (23.50%), Postives = 92/200 (46.00%), Query Frame = 0


Query: 52  NTTLTHDELTRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDF 111
           N  ++HD L    +L+   C  + LS A++LL   +        ++L  L +     + F
Sbjct: 107 NLGISHD-LYSFTILIHCFCRCSRLSLALALLGKMMKLGFRPSIVTLGSLLNGFCQGNRF 166

Query: 112 ALTMSLLTRLKHH---PNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPE 171
              +SL+  +      PN ++Y+T     +I+  CK R    AL++F+ M + G   + +
Sbjct: 167 QEAVSLVDSMDGFGFVPNVVIYNT-----VINGLCKNRDLNNALEVFYCMEKKG--IRAD 226

Query: 172 ERVYKTLIAGLYRKGMTFDALKVLRNMIDSNLVPD-----------------CDLRNWVF 231
              Y TLI+GL   G   DA ++LR+M+   + P+                  + RN ++
Sbjct: 227 AVTYNTLISGLSNSGRWTDAARLLRDMVKRKIDPNVIFFTALIDTFVKEGNLLEARN-LY 286

[Close]

BLAST of Cucsat.G14820.T4 vs. ExPASy Swiss-Prot
Match: Q6NQ83 (Pentatricopeptide repeat-containing protein At3g22470, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g22470 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 3.4e-05
Identity = 44/167 (26.35%), Postives = 76/167 (45.51%), Query Frame = 0


Query: 59  ELTRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLL 118
           +L  ++ L+  LCL   +S A+ L+   +        ++   + + L    + AL + L 
Sbjct: 174 DLVTVSTLINGLCLKGRVSEALVLIDRMVEYGFQPDEVTYGPVLNRLCKSGNSALALDLF 233

Query: 119 TRLKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAG 178
            +++     +  S    +++I S CK     +AL LF+ M   G   K +   Y +LI G
Sbjct: 234 RKMEE--RNIKASVVQYSIVIDSLCKDGSFDDALSLFNEMEMKG--IKADVVTYSSLIGG 293

Query: 179 LYRKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAME 226
           L   G   D  K+LR MI  N++PD    + +    +KE  + EA E
Sbjct: 294 LCNDGKWDDGAKMLREMIGRNIIPDVVTFSALIDVFVKEGKLLEAKE 336

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BLAST of Cucsat.G14820.T4 vs. NCBI nr
Match: XP_004154059.1 (putative pentatricopeptide repeat-containing protein At5g59900 [Cucumis sativus] >KGN64453.1 hypothetical protein Csa_014366 [Cucumis sativus])

HSP 1 Score: 493 bits (1269), Expect = 2.01e-175
Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 0


Query: 1   MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSPSASIATKPNTTLTHDEL 60
           MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSPSASIATKPNTTLTHDEL
Sbjct: 1   MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSPSASIATKPNTTLTHDEL 60

Query: 61  TRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTR 120
           TRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTR
Sbjct: 61  TRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTR 120

Query: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY 180
           LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY
Sbjct: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY 180

Query: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNTID 240
           RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNTID
Sbjct: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNTID 240

Query: 241 HLRRVSELLNRIITNWID 258
           HLRRVSELLNRIITNWID
Sbjct: 241 HLRRVSELLNRIITNWID 258

[Close]

BLAST of Cucsat.G14820.T4 vs. NCBI nr
Match: KAA0055365.1 (protein PLASTID TRANSCRIPTIONALLY ACTIVE 14 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 443 bits (1140), Expect = 8.15e-149
Identity = 232/245 (94.69%), Postives = 239/245 (97.55%), Query Frame = 0


Query: 1   MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSPSASIATKPNTTLTHDEL 60
           MKRIRQQLLTLQRLPLSPELLRFQIP ILSPFSSSSSFIS SPSASIAT+PNTTLTHDEL
Sbjct: 1   MKRIRQQLLTLQRLPLSPELLRFQIPPILSPFSSSSSFISGSPSASIATEPNTTLTHDEL 60

Query: 61  TRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTR 120
           TRINLLLPRLCL+NHLSTAI+LLHATLLTNPSL SLSLSVLSHSLASQSDFALTMSLLTR
Sbjct: 61  TRINLLLPRLCLYNHLSTAITLLHATLLTNPSLQSLSLSVLSHSLASQSDFALTMSLLTR 120

Query: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY 180
           LKHHPNALLYSTPIVTMLISSYCKRRKSKEALK+FHWMLRPGSPCKPEERVYKTLIAGLY
Sbjct: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKIFHWMLRPGSPCKPEERVYKTLIAGLY 180

Query: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNTID 240
           RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFR LL+EAMIPEAMEFN+  NFVGDQ+TID
Sbjct: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRCLLREAMIPEAMEFNETFNFVGDQDTID 240

Query: 241 HLRRV 245
           HLRRV
Sbjct: 241 HLRRV 245

[Close]

BLAST of Cucsat.G14820.T4 vs. NCBI nr
Match: TYJ99292.1 (protein PLASTID TRANSCRIPTIONALLY ACTIVE 14 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 443 bits (1140), Expect = 2.19e-148
Identity = 232/245 (94.69%), Postives = 239/245 (97.55%), Query Frame = 0


Query: 1   MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSPSASIATKPNTTLTHDEL 60
           MKRIRQQLLTLQRLPLSPELLRFQIP ILSPFSSSSSFIS SPSASIAT+PNTTLTHDEL
Sbjct: 1   MKRIRQQLLTLQRLPLSPELLRFQIPPILSPFSSSSSFISGSPSASIATEPNTTLTHDEL 60

Query: 61  TRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTR 120
           TRINLLLPRLCL+NHLSTAI+LLHATLLTNPSL SLSLSVLSHSLASQSDFALTMSLLTR
Sbjct: 61  TRINLLLPRLCLYNHLSTAITLLHATLLTNPSLQSLSLSVLSHSLASQSDFALTMSLLTR 120

Query: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY 180
           LKHHPNALLYSTPIVTMLISSYCKRRKSKEALK+FHWMLRPGSPCKPEERVYKTLIAGLY
Sbjct: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKIFHWMLRPGSPCKPEERVYKTLIAGLY 180

Query: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNTID 240
           RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFR LL+EAMIPEAMEFN+  NFVGDQ+TID
Sbjct: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRCLLREAMIPEAMEFNETFNFVGDQDTID 240

Query: 241 HLRRV 245
           HLRRV
Sbjct: 241 HLRRV 245

[Close]

BLAST of Cucsat.G14820.T4 vs. NCBI nr
Match: XP_038876976.1 (pentatricopeptide repeat-containing protein At3g22470, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 389 bits (999), Expect = 2.95e-134
Identity = 210/260 (80.77%), Postives = 230/260 (88.46%), Query Frame = 0


Query: 1   MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSP-SASIATKPNTTLTHDE 60
           MKRIRQ+LL L RLPL P+LL +QIPAI S  SSSSS    +P SASIA++PNTTLTHDE
Sbjct: 1   MKRIRQRLLPLHRLPLPPQLLCYQIPAIPSSSSSSSSISDSAPASASIASEPNTTLTHDE 60

Query: 61  L-TRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLL 120
           L T+INLLLPRLC +NH STAI+LL ATLLTNPSL SL LS+LSHSLASQSDFALTMSLL
Sbjct: 61  LITKINLLLPRLCFYNHHSTAIALLDATLLTNPSLQSLPLSILSHSLASQSDFALTMSLL 120

Query: 121 TRLKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAG 180
           TRL HH NALLYSTPI+TMLI SY KRRKSK+ALK+FHWMLRPGSPCKP+ERVY TLIAG
Sbjct: 121 TRLNHHRNALLYSTPIITMLIFSYSKRRKSKDALKIFHWMLRPGSPCKPDERVYNTLIAG 180

Query: 181 LYRKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNT 240
           L RKGMT DALKVLRNM++SNLVPDCDLRNWVFR LL+EAMI EAME N+ LNFVGDQNT
Sbjct: 181 LCRKGMTLDALKVLRNMVNSNLVPDCDLRNWVFRCLLREAMIGEAMELNETLNFVGDQNT 240

Query: 241 IDHLRRVSELLNRIITNWID 258
            DHLR+VSELL+RIITNWID
Sbjct: 241 SDHLRKVSELLDRIITNWID 260

[Close]

BLAST of Cucsat.G14820.T4 vs. NCBI nr
Match: KAG6584178.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 353 bits (905), Expect = 5.19e-120
Identity = 192/258 (74.42%), Postives = 219/258 (84.88%), Query Frame = 0


Query: 1   MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSPSASIATKPNTTLTHDEL 60
           MK +RQ+LL L RLPL P+L+  QIPAI +PFSSSSS  S S   S A+K NTTLT DEL
Sbjct: 1   MKTVRQRLLHLHRLPLPPQLVHDQIPAIGTPFSSSSS--SSSVYDSAASKINTTLTQDEL 60

Query: 61  TRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTR 120
           T+I+LLLPRLC +NH STAI+LL   LLTN SL SL LS+LSHSLASQSDFALTMSLLTR
Sbjct: 61  TKISLLLPRLCFYNHHSTAITLLDTALLTNLSLQSLPLSILSHSLASQSDFALTMSLLTR 120

Query: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY 180
           LKHH NALLYS+PI+TMLI SY ++RK KEALK+FHWMLRPGSPCKP+ERVYKTLIAGL 
Sbjct: 121 LKHHRNALLYSSPIITMLILSYSRQRKLKEALKIFHWMLRPGSPCKPDERVYKTLIAGLC 180

Query: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNTID 240
           RKGM  DALK+L+NMIDSNLVPD DLR+WVFR LL+EAMI EA E ++ LN +GD+NT D
Sbjct: 181 RKGMVLDALKILKNMIDSNLVPDYDLRSWVFRCLLREAMIGEATELSETLNSIGDRNTSD 240

Query: 241 HLRRVSELLNRIITNWID 258
           HLRRVSELL+RIITNWI+
Sbjct: 241 HLRRVSELLDRIITNWIE 256

[Close]

BLAST of Cucsat.G14820.T4 vs. ExPASy TrEMBL
Match: A0A0A0LRQ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G051900 PE=4 SV=1)

HSP 1 Score: 493 bits (1269), Expect = 9.74e-176
Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 0


Query: 1   MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSPSASIATKPNTTLTHDEL 60
           MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSPSASIATKPNTTLTHDEL
Sbjct: 1   MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSPSASIATKPNTTLTHDEL 60

Query: 61  TRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTR 120
           TRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTR
Sbjct: 61  TRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTR 120

Query: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY 180
           LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY
Sbjct: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY 180

Query: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNTID 240
           RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNTID
Sbjct: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNTID 240

Query: 241 HLRRVSELLNRIITNWID 258
           HLRRVSELLNRIITNWID
Sbjct: 241 HLRRVSELLNRIITNWID 258

[Close]

BLAST of Cucsat.G14820.T4 vs. ExPASy TrEMBL
Match: A0A5A7UP05 (Protein PLASTID TRANSCRIPTIONALLY ACTIVE 14 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G002100 PE=4 SV=1)

HSP 1 Score: 443 bits (1140), Expect = 3.95e-149
Identity = 232/245 (94.69%), Postives = 239/245 (97.55%), Query Frame = 0


Query: 1   MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSPSASIATKPNTTLTHDEL 60
           MKRIRQQLLTLQRLPLSPELLRFQIP ILSPFSSSSSFIS SPSASIAT+PNTTLTHDEL
Sbjct: 1   MKRIRQQLLTLQRLPLSPELLRFQIPPILSPFSSSSSFISGSPSASIATEPNTTLTHDEL 60

Query: 61  TRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTR 120
           TRINLLLPRLCL+NHLSTAI+LLHATLLTNPSL SLSLSVLSHSLASQSDFALTMSLLTR
Sbjct: 61  TRINLLLPRLCLYNHLSTAITLLHATLLTNPSLQSLSLSVLSHSLASQSDFALTMSLLTR 120

Query: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY 180
           LKHHPNALLYSTPIVTMLISSYCKRRKSKEALK+FHWMLRPGSPCKPEERVYKTLIAGLY
Sbjct: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKIFHWMLRPGSPCKPEERVYKTLIAGLY 180

Query: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNTID 240
           RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFR LL+EAMIPEAMEFN+  NFVGDQ+TID
Sbjct: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRCLLREAMIPEAMEFNETFNFVGDQDTID 240

Query: 241 HLRRV 245
           HLRRV
Sbjct: 241 HLRRV 245

[Close]

BLAST of Cucsat.G14820.T4 vs. ExPASy TrEMBL
Match: A0A5D3BHR6 (Protein PLASTID TRANSCRIPTIONALLY ACTIVE 14 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005050 PE=4 SV=1)

HSP 1 Score: 443 bits (1140), Expect = 1.06e-148
Identity = 232/245 (94.69%), Postives = 239/245 (97.55%), Query Frame = 0


Query: 1   MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSPSASIATKPNTTLTHDEL 60
           MKRIRQQLLTLQRLPLSPELLRFQIP ILSPFSSSSSFIS SPSASIAT+PNTTLTHDEL
Sbjct: 1   MKRIRQQLLTLQRLPLSPELLRFQIPPILSPFSSSSSFISGSPSASIATEPNTTLTHDEL 60

Query: 61  TRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTR 120
           TRINLLLPRLCL+NHLSTAI+LLHATLLTNPSL SLSLSVLSHSLASQSDFALTMSLLTR
Sbjct: 61  TRINLLLPRLCLYNHLSTAITLLHATLLTNPSLQSLSLSVLSHSLASQSDFALTMSLLTR 120

Query: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY 180
           LKHHPNALLYSTPIVTMLISSYCKRRKSKEALK+FHWMLRPGSPCKPEERVYKTLIAGLY
Sbjct: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKIFHWMLRPGSPCKPEERVYKTLIAGLY 180

Query: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNTID 240
           RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFR LL+EAMIPEAMEFN+  NFVGDQ+TID
Sbjct: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRCLLREAMIPEAMEFNETFNFVGDQDTID 240

Query: 241 HLRRV 245
           HLRRV
Sbjct: 241 HLRRV 245

[Close]

BLAST of Cucsat.G14820.T4 vs. ExPASy TrEMBL
Match: A0A6J1E820 (pentatricopeptide repeat-containing protein At3g22470, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111431498 PE=4 SV=1)

HSP 1 Score: 344 bits (882), Expect = 7.93e-117
Identity = 190/260 (73.08%), Postives = 216/260 (83.08%), Query Frame = 0


Query: 1   MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFS--SSSSFISDSPSASIATKPNTTLTHD 60
           MK I Q+LL L RLPL P+L+  QIPAI +PFS  SSSS + DS     A+K NTTLT D
Sbjct: 1   MKTITQRLLPLHRLPLPPQLVHDQIPAIGTPFSFFSSSSSVYDSA----ASKINTTLTQD 60

Query: 61  ELTRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLL 120
           ELT+I+LLLPRLC +NH STAI+LL   LLTN SL SL LS+LSHSLASQSDFALTMSLL
Sbjct: 61  ELTKISLLLPRLCFYNHHSTAITLLDTALLTNLSLQSLPLSILSHSLASQSDFALTMSLL 120

Query: 121 TRLKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAG 180
           TRLKHH NALLYS+PI+TMLI SY K+RK KEALK+FHWMLRPGSPCKP+ERVYKTLIAG
Sbjct: 121 TRLKHHRNALLYSSPIITMLILSYSKQRKLKEALKIFHWMLRPGSPCKPDERVYKTLIAG 180

Query: 181 LYRKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNT 240
           L RKGM  DALK+L+NMIDSNLVPD DLR+WVFR LL+EAMI EA E ++ LN +GD+NT
Sbjct: 181 LCRKGMVLDALKILKNMIDSNLVPDYDLRSWVFRCLLREAMIGEATELSETLNSIGDRNT 240

Query: 241 IDHLRRVSELLNRIITNWID 258
            DHLRRVSELL+ II NWI+
Sbjct: 241 SDHLRRVSELLDGIINNWIE 256

[Close]

BLAST of Cucsat.G14820.T4 vs. ExPASy TrEMBL
Match: A0A6J1KEJ7 (pentatricopeptide repeat-containing protein At3g22470, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111495112 PE=4 SV=1)

HSP 1 Score: 343 bits (880), Expect = 1.60e-116
Identity = 188/258 (72.87%), Postives = 216/258 (83.72%), Query Frame = 0


Query: 1   MKRIRQQLLTLQRLPLSPELLRFQIPAILSPFSSSSSFISDSPSASIATKPNTTLTHDEL 60
           MK IRQ+LL L+RLPL P+L+  QIPAI +PFSSSSS  S S   S A+K NTTLT DEL
Sbjct: 1   MKTIRQRLLPLRRLPLPPQLVHDQIPAIGTPFSSSSS--SSSVYDSAASKINTTLTQDEL 60

Query: 61  TRINLLLPRLCLHNHLSTAISLLHATLLTNPSLHSLSLSVLSHSLASQSDFALTMSLLTR 120
           T+I+LLLPRLCL+NH STAI+LL   LLTN SL SL LS+LSHSLASQSDFALTMSLLTR
Sbjct: 61  TKISLLLPRLCLYNHHSTAITLLDTALLTNLSLQSLPLSILSHSLASQSDFALTMSLLTR 120

Query: 121 LKHHPNALLYSTPIVTMLISSYCKRRKSKEALKLFHWMLRPGSPCKPEERVYKTLIAGLY 180
           LKHH NALLYS+PI+TMLI SY K+RKSKEALK+FHWMLRPGSPCKP+E VYKTLIAGL 
Sbjct: 121 LKHHRNALLYSSPIITMLIFSYSKQRKSKEALKIFHWMLRPGSPCKPDEMVYKTLIAGLC 180

Query: 181 RKGMTFDALKVLRNMIDSNLVPDCDLRNWVFRSLLKEAMIPEAMEFNDALNFVGDQNTID 240
           RKGM  DALK+L+NMIDSNLVPD DLR+WV   LL+EAMI EA E ++ LN + D+N  D
Sbjct: 181 RKGMVLDALKILKNMIDSNLVPDYDLRSWVCMCLLREAMIGEATELSETLNSIADRNISD 240

Query: 241 HLRRVSELLNRIITNWID 258
           HLR+VSELL+R+I NWI+
Sbjct: 241 HLRKVSELLDRVINNWIE 256

The following BLAST results are available for this feature:

BLAST of Cucsat.G14820.T4 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJE6	1.8e-06	26.51	Putative pentatricopeptide repeat-containing protein At5g59900 OS=Arabidopsis th...	[more]
Q9FND8	2.4e-06	27.07	Pentatricopeptide repeat-containing protein At5g40400 OS=Arabidopsis thaliana OX...	[more]
Q3EDF8	1.5e-05	26.51	Pentatricopeptide repeat-containing protein At1g09900 OS=Arabidopsis thaliana OX...	[more]
Q0WKZ3	2.0e-05	23.50	Pentatricopeptide repeat-containing protein At1g64580 OS=Arabidopsis thaliana OX...	[more]
Q6NQ83	3.4e-05	26.35	Pentatricopeptide repeat-containing protein At3g22470, mitochondrial OS=Arabidop...	[more]

BLAST of Cucsat.G14820.T4 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004154059.1	2.01e-175	100.00	putative pentatricopeptide repeat-containing protein At5g59900 [Cucumis sativus]...	[more]
KAA0055365.1	8.15e-149	94.69	protein PLASTID TRANSCRIPTIONALLY ACTIVE 14 isoform X2 [Cucumis melo var. makuwa...	[more]
TYJ99292.1	2.19e-148	94.69	protein PLASTID TRANSCRIPTIONALLY ACTIVE 14 isoform X1 [Cucumis melo var. makuwa...	[more]
XP_038876976.1	2.95e-134	80.77	pentatricopeptide repeat-containing protein At3g22470, mitochondrial-like [Benin...	[more]
KAG6584178.1	5.19e-120	74.42	Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a...	[more]

BLAST of Cucsat.G14820.T4 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRQ9	9.74e-176	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G051900 PE=4 SV=1	[more]
A0A5A7UP05	3.95e-149	94.69	Protein PLASTID TRANSCRIPTIONALLY ACTIVE 14 isoform X2 OS=Cucumis melo var. maku...	[more]
A0A5D3BHR6	1.06e-148	94.69	Protein PLASTID TRANSCRIPTIONALLY ACTIVE 14 isoform X1 OS=Cucumis melo var. maku...	[more]
A0A6J1E820	7.93e-117	73.08	pentatricopeptide repeat-containing protein At3g22470, mitochondrial-like OS=Cuc...	[more]
A0A6J1KEJ7	1.60e-116	72.87	pentatricopeptide repeat-containing protein At3g22470, mitochondrial-like OS=Cuc...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002885	Pentatricopeptide repeat	TIGRFAM	TIGR00756	TIGR00756	coord: 171..204 e-value: 5.5E-6 score: 24.2 coord: 136..156 e-value: 6.6E-4 score: 17.7
IPR002885	Pentatricopeptide repeat	PFAM	PF01535	PPR	coord: 136..156 e-value: 8.7E-4 score: 19.4 coord: 171..199 e-value: 0.0013 score: 18.9
IPR002885	Pentatricopeptide repeat	PROSITE	PS51375	PPR	coord: 131..165 score: 9.04313
IPR002885	Pentatricopeptide repeat	PROSITE	PS51375	PPR	coord: 168..202 score: 9.656963
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 61..256 e-value: 2.4E-18 score: 68.6
None	No IPR available	PANTHER	PTHR47932	ATPASE EXPRESSION PROTEIN 3	coord: 166..236
None	No IPR available	PANTHER	PTHR47932:SF1	SUBFAMILY NOT NAMED	coord: 166..236

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G14820	Cucsat.G14820	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G14820.T4.E1	Cucsat.G14820.T4.E1	exon
Cucsat.G14820.T4.E2	Cucsat.G14820.T4.E2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G14820.T4.C1	Cucsat.G14820.T4.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G14820.T4	Cucsat.G14820.T4-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009658	chloroplast organization
biological_process	GO:0018026	peptidyl-lysine monomethylation
biological_process	GO:0042793	plastid transcription
biological_process	GO:0009416	response to light stimulus
biological_process	GO:0010027	thylakoid membrane organization
cellular_component	GO:0009534	chloroplast thylakoid
cellular_component	GO:0000427	plastid-encoded plastid RNA polymerase complex
molecular_function	GO:0005515	protein binding
molecular_function	GO:0016279	protein-lysine N-methyltransferase activity