Cucsat.G1469.T1 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G1469.T1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Pyruvate dehydrogenase E1 component subunit alpha
Location	ctg10: 149975 .. 153036 (-)
RNA-Seq Expression	Cucsat.G1469.T1
Synteny	Cucsat.G1469.T1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTGATTTGGTTTGAGAGTAAAATTCTCATAAATACACACACTTTAATATTTAGATAAATATGTGAAGAAAATTAGGAAGTAGTTGGTTTTAGTTGAGTGGCTTTCTTCTTTCTCCTCCGTTTCAATTTCATCTTTGTGGCTTTTGCAGTGAAAAAACGAACACCAAAATCCTCACGCCTCAGAAAAGGCGGCGATTTCCAAGCTCCTTCTCCTCGTTTCCTCCGCCCCTTTCTTCATTCTCTTCCCCTTTTTATATCTTTCTCTTCTTTTCTCTTTTCTTTCATTTTCCCATCTCTCCTTTTTCCCATTTACTCCCTTCTTCTTTCTCACACACAATGTCCTTCTCTTCATCACTCAAAATCCTCCAACCCCTTCCTCTTAACTCCACCAGATCCAATGACAAACCCCTTTTATTTGATCCCTTTAGATCCACCTCCAAGTTTCTTGGATCTCGATTTCGTCTCCCTTCTCTCTCTAAATCCAATACTCGTTGTCGATCTTCCCCTGTTGTTGCCGTTTCCGATGTTGTCAAGGAGAAGAAGCTCAAACCCAGTTCCAATTTGGTACTTTTTTTTTTTTTTTTTTTTTTTAATTCTAGATTCTGAATTTGTTGTATTGATTGATTTGTTATTTGGGTTTTTAATGTCCTGCAAATTATGGGGGGATTTGGTAATGTTGCATGACATTTTAGCTTTAACGATTTTGCTTTTGTTGGTTTTGGTTTCCCTGTTGTTATTTTCTTTTGGAAAATTGGATGGTTTGATTGAGATTGTCTTTTTGAAATTGGAAGTTATATTGTTCTTTTGTCCCATCAGTGGAATTAAAATTTTACTCTACTGAAGCTTTTGTGTGCATTGCTTTGCTGCATGAAGCATCTTGGTTTAAAAGTTCTTCACTTTTTTTGGTTCCTTTTATTGTCTCCACACAGCTGATTACAAAAGAAGAGGGTTTGGTACTCTATGAGGACATGATATTAGGCAGAGAATTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCCACTGGGTTCATCAAGCTTCTTACACAGAGGGATACTGTAGTAAGCACTTATCGTGATCACGTTCATGCTCTCAGTAAGGGAGTTCCATCTCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGGTGCTGCCGTGGCCAAGGTGGCTCAATGCATATGTTCTCAAAAGAACATAATCTGATTGGGGGATTTGCCTTCATTGGAGAAGGCATACCAGTGGCCACTGGTGCTGCATTCACGTCTAAATACAAGAGAGAAGTTTTAAAAGAGGCAGACTGTCAAGATGTGACATTGGCATTTTTCGGGGATGGAACTTGCAACAATGGCCAGTTCTTCGAGTGCTTGAACATGGCAGCTCTGTGGAAATTGCCAATCGTATTTGTTGTGGAGAATAATCTTTGGGCAATTGGAATGTCGCATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAAAAAGGTCCTGCATTTGGAATGCCTGGTGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGAGAGGTTGCAAAGGAGGCAATTGGAAGAGCCAGAAGAGGAGAAGGACCGACTCTCGTGGAATGTGAAACCTATAGGTTTAGAGGACACTCTCTGGCTGATCCAGATGAACTCCGTGACCCAGGTTAGTGCTCTTAAATCCATACCTACAATTGGGTCTGTCATATTCAAAATATGCCTTTGCTTGGCCTTCTTGACATTTATTATTACGGCTAGTCAAAGTTCCTTAATTCATTTCTCGTGAATATGATAGCTGATCTCTTAATTCGTACTCCATCATAGCCATTTAAGGATAATTCATTCAGGGTTGTTAATGAGCTGCACCGCTTGCAATTCACTCATTAGTTAACTCGAAATTTTTTCTCATTTGTTAATTAGATTTTTTGTTTTTCTTTATTTTTTAAATGGAATCAACTAACTTTGATTGAAAAAAAAAATGTAAGAATACAAGGACATACGTCCACCAAAGCACCCCTTCTAAAGAAAGGGCTTCCACAACTGAGTAAGATATTACTTAGAGAATAATTACAAAAGAAAAAAAAGCTTTGAAACTGAAGCACGAAGCGAGACATGAAGACTCACGAGGAATCAAATCTCACTCGTGTTCCTGTTCAAACCTCTAAACACACTAGTGTTCCTCTTCCCTCGAATATCCCACAATAAAGCTGATTCTTCATAGATGATAGAAATTGATGTCCCCATCAGGTTCTTCATTGTACCTTACACGCACAAATGTAGTAGTTTCTTATCTAAGTATAGTAACTCCGGAAAGTACAAAGGATTAAGAATTCCCTGATTTAACCATTGCAGATGAGAAGGCTCGTTATGCTGCCAGAGATCCCATTGCAGCTTTGAAGAAGTACATGTTGGAGAATAAACTAGCCAATGAACAGGAGCTAAAGGCAATAAAGGATAAGATAGTTGAGGTGGTTGAGGAGGCAGTTCAATTTGCAGATGAAAGCCCCCATCCAGCAAGAAGCCAGTTATTAGAGAATGTCTTTGCCGATCCTAAAGGCTTTGGAATTGGGCCTGATGGAAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGAACTGCACATGTCTAAACTTCACGAACTTTGTTGTCTATCTAAGTCTTTTTATTTATTTTTATTTGTTTTCTTTACTGTTCCTGTTGGGTTATTTTCTGATTATTTGTAATTGAATGTTAAATTAGCTTTTTTCTGAAGCAACTAGGAATTTCCTTCCTTTCTTCAGGTTGTGCTGTAGTTTTTTTTTTCTTTCTTTTTCTTCTTTTTCTTTTTACCAATTTTGATTTAGTGTATGATACAAACATTCCTCTACTAATCTAGTTATGTGGGAGATGGAAGCATTGTTTAATGCTTTACATATTATGATCATGAGCAGTTTAAACGCTATCGTAGTTTTGGAACTTTTATACTTCATACTTCATATTCTGTTTTCATCCTTCTACTCTGGTTTTTGCTCCTTTTG

Coding sequence (CDS)

ATGTCCTTCTCTTCATCACTCAAAATCCTCCAACCCCTTCCTCTTAACTCCACCAGATCCAATGACAAACCCCTTTTATTTGATCCCTTTAGATCCACCTCCAAGTTTCTTGGATCTCGATTTCGTCTCCCTTCTCTCTCTAAATCCAATACTCGTTGTCGATCTTCCCCTGTTGTTGCCGTTTCCGATGTTGTCAAGGAGAAGAAGCTCAAACCCAGTTCCAATTTGCTGATTACAAAAGAAGAGGGTTTGGTACTCTATGAGGACATGATATTAGGCAGAGAATTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCCACTGGGTTCATCAAGCTTCTTACACAGAGGGATACTGTAGTAAGCACTTATCGTGATCACGTTCATGCTCTCAGTAAGGGAGTTCCATCTCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGGTGCTGCCGTGGCCAAGGTGGCTCAATGCATATGTTCTCAAAAGAACATAATCTGATTGGGGGATTTGCCTTCATTGGAGAAGGCATACCAGTGGCCACTGGTGCTGCATTCACGTCTAAATACAAGAGAGAAGTTTTAAAAGAGGCAGACTGTCAAGATGTGACATTGGCATTTTTCGGGGATGGAACTTGCAACAATGGCCAGTTCTTCGAGTGCTTGAACATGGCAGCTCTGTGGAAATTGCCAATCGTATTTGTTGTGGAGAATAATCTTTGGGCAATTGGAATGTCGCATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAAAAAGGTCCTGCATTTGGAATGCCTGGTGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGAGAGGTTGCAAAGGAGGCAATTGGAAGAGCCAGAAGAGGAGAAGGACCGACTCTCGTGGAATGTGAAACCTATAGGTTTAGAGGACACTCTCTGGCTGATCCAGATGAACTCCGTGACCCAGATGAGAAGGCTCGTTATGCTGCCAGAGATCCCATTGCAGCTTTGAAGAAGTACATGTTGGAGAATAAACTAGCCAATGAACAGGAGCTAAAGGCAATAAAGGATAAGATAGTTGAGGTGGTTGAGGAGGCAGTTCAATTTGCAGATGAAAGCCCCCATCCAGCAAGAAGCCAGTTATTAGAGAATGTCTTTGCCGATCCTAAAGGCTTTGGAATTGGGCCTGATGGAAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGAACTGCACATGTCTAA

Protein sequence

MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVAVSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKLANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTEGTAHV

Homology

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BLAST of Cucsat.G1469.T1 vs. ExPASy Swiss-Prot
Match: O24457 (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PDH-E1 ALPHA PE=2 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 7.3e-194
Identity = 340/423 (80.38%), Postives = 372/423 (87.94%), Query Frame = 0


Query: 8   KILQPLPLNSTRSNDKPLLFDPFRSTSKFLGS--RFRLPSLSKSNTRCRSSPVVAVSDVV 67
           K+   +PL+ +  N + LL       S FLGS     L  L+ SN   R SPVV+V +VV
Sbjct: 9   KLTATVPLHGSHEN-RLLLPIRLAPPSSFLGSTRSLSLRRLNHSNA-TRRSPVVSVQEVV 68

Query: 68  KEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTG 127
           KEK+   +++LLITKEEGL LYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTG
Sbjct: 69  KEKQSTNNTSLLITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVSTG 128

Query: 128 FIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLI 187
           FIKLLT+ D+VVSTYRDHVHALSKGV +R VMSELFGK TGCCRGQGGSMHMFSKEHN++
Sbjct: 129 FIKLLTKSDSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHNML 188

Query: 188 GGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALW 247
           GGFAFIGEGIPVATGAAF+SKY+REVLK+ DC DVT+AFFGDGTCNNGQFFECLNMAAL+
Sbjct: 189 GGFAFIGEGIPVATGAAFSSKYRREVLKQ-DCDDVTVAFFGDGTCNNGQFFECLNMAALY 248

Query: 248 KLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGR 307
           KLPI+FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEA+ R
Sbjct: 249 KLPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAVTR 308

Query: 308 ARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKLANEQE 367
           ARRGEGPTLVECETYRFRGHSLADPDELRD  EKA+YAARDPIAALKKY++ENKLA E E
Sbjct: 309 ARRGEGPTLVECETYRFRGHSLADPDELRDAAEKAKYAARDPIAALKKYLIENKLAKEAE 368

Query: 368 LKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTEGT 427
           LK+I+ KI E+VEEAV+FAD SP P RSQLLENVFADPKGFGIGPDG+YRCEDPKFTEGT
Sbjct: 369 LKSIEKKIDELVEEAVEFADASPQPGRSQLLENVFADPKGFGIGPDGRYRCEDPKFTEGT 428

Query: 428 AHV 429
           A V
Sbjct: 429 AQV 428

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BLAST of Cucsat.G1469.T1 vs. ExPASy Swiss-Prot
Match: Q7XTJ3 (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0119400 PE=2 SV=2)

HSP 1 Score: 629.8 bits (1623), Expect = 2.3e-179
Identity = 320/431 (74.25%), Postives = 355/431 (82.37%), Query Frame = 0


Query: 2   SFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVAV 61
           SF+++ K L P+   S      PL   P  +++     R       K   R R++  V+ 
Sbjct: 6   SFTAAAKFLAPVSARSAGDYKPPL---PLPASASLRPGR-------KPAPRLRTALAVS- 65

Query: 62  SDVVKEKKLKP--SSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQ 121
           SDV+   K  P  +++  +T+EE L LYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQ
Sbjct: 66  SDVLPGNKAAPAAAAHSAVTREEALELYEDMVLGRIFEDMCAQMYYRGKMFGFVHLYNGQ 125

Query: 122 EAVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFS 181
           EAVSTGFIKLL Q D VVSTYRDHVHALSKGVP+R VM+ELFGK TGCCRGQGGSMHMFS
Sbjct: 126 EAVSTGFIKLLNQADCVVSTYRDHVHALSKGVPARSVMAELFGKATGCCRGQGGSMHMFS 185

Query: 182 KEHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEA--DCQDVTLAFFGDGTCNNGQFFE 241
           + HNL+GGFAFIGEGIPVATGAAF +KY+ EVLK++  D  DVTLAFFGDGTCNNGQFFE
Sbjct: 186 EPHNLLGGFAFIGEGIPVATGAAFAAKYRHEVLKQSSPDGLDVTLAFFGDGTCNNGQFFE 245

Query: 242 CLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVRE 301
           CLNMA LWKLPIVFVVENNLWAIGMSHLRATSDPEI+KKGPAFGMPGVHVDGMDVLKVRE
Sbjct: 246 CLNMAQLWKLPIVFVVENNLWAIGMSHLRATSDPEIYKKGPAFGMPGVHVDGMDVLKVRE 305

Query: 302 VAKEAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLE 361
           VAKEAI RARRGEGPTLVECETYRFRGHSLADPDELR PDEK+ YAARDPI ALKKY++E
Sbjct: 306 VAKEAIERARRGEGPTLVECETYRFRGHSLADPDELRRPDEKSHYAARDPITALKKYIIE 365

Query: 362 NKLANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCE 421
             LA E ELK+I+ KI +VVEEAV+FAD SP P RSQLLENVF+DPKGFGIGPDGKYRCE
Sbjct: 366 QNLATESELKSIEKKIDDVVEEAVEFADASPLPPRSQLLENVFSDPKGFGIGPDGKYRCE 425

Query: 422 DPKFTEGTAHV 429
           DP FT+GTA V
Sbjct: 426 DPLFTQGTAQV 425

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BLAST of Cucsat.G1469.T1 vs. ExPASy Swiss-Prot
Match: Q1XDM0 (Pyruvate dehydrogenase E1 component subunit alpha OS=Pyropia yezoensis OX=2788 GN=pdhA PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 9.7e-130
Identity = 225/330 (68.18%), Postives = 264/330 (80.00%), Query Frame = 0


Query: 73  SSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTQ 132
           S+ L + K   LVLYEDM+LGR FEDMCAQMYY+GKMFGFVHLYNGQEAVSTG IKLL  
Sbjct: 16  STGLNLNKSNLLVLYEDMLLGRNFEDMCAQMYYKGKMFGFVHLYNGQEAVSTGVIKLLNP 75

Query: 133 RDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIG 192
            D V STYRDHVHALSKGVPS+ VM+ELFGK TGC +G+GGSMH+FS  HN +GGFAFI 
Sbjct: 76  TDYVCSTYRDHVHALSKGVPSKNVMAELFGKETGCSKGRGGSMHIFSAPHNFLGGFAFIA 135

Query: 193 EGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFV 252
           EGIPVATGAAF S Y+++VLKE +   VT  FFGDGT NNGQFFECLNMA LWKLPI+FV
Sbjct: 136 EGIPVATGAAFQSIYRQQVLKETEDLRVTACFFGDGTTNNGQFFECLNMAVLWKLPIIFV 195

Query: 253 VENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGP 312
           VENN WAIGM+H R++S PEI KK  AFG+PG+ VDGMDVL VR+ AK+A+ RAR+G+GP
Sbjct: 196 VENNQWAIGMAHHRSSSIPEIHKKAEAFGLPGIEVDGMDVLAVRQAAKQAVQRARQGDGP 255

Query: 313 TLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKLANEQELKAIKDK 372
           TL+E  TYRFRGHSLADPDELR   EK  + ARDPI  LKKY+L+N++AN  EL  I++ 
Sbjct: 256 TLIEALTYRFRGHSLADPDELRSRQEKEAWVARDPIKKLKKYILDNEIANIGELNEIQNA 315

Query: 373 IVEVVEEAVQFADESPHPARSQLLENVFAD 403
           +   +E+AV+FA  SP P  S+L   +FAD
Sbjct: 316 VKTELEQAVKFAISSPEPNMSELKRYLFAD 345

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BLAST of Cucsat.G1469.T1 vs. ExPASy Swiss-Prot
Match: P51267 (Pyruvate dehydrogenase E1 component subunit alpha OS=Porphyra purpurea OX=2787 GN=pdhA PE=3 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 4.1e-128
Identity = 224/336 (66.67%), Postives = 267/336 (79.46%), Query Frame = 0


Query: 67  EKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGF 126
           E  L   + + +TK + LVLYEDM+LGR FEDMCAQMYY+GKMFGFVHLYNGQEAVSTG 
Sbjct: 8   ELPLTNCNQINLTKHKLLVLYEDMLLGRNFEDMCAQMYYKGKMFGFVHLYNGQEAVSTGV 67

Query: 127 IKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIG 186
           IKLL  +D V STYRDHVHALSKGVPS+ VM+ELFGK TGC RG+GGSMH+FS  HN +G
Sbjct: 68  IKLLDSKDYVCSTYRDHVHALSKGVPSQNVMAELFGKETGCSRGRGGSMHIFSAPHNFLG 127

Query: 187 GFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWK 246
           GFAFI EGIPVATGAAF S Y+++VLKE     VT  FFGDGT NNGQFFECLNMA LWK
Sbjct: 128 GFAFIAEGIPVATGAAFQSIYRQQVLKEPGELRVTACFFGDGTTNNGQFFECLNMAVLWK 187

Query: 247 LPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRA 306
           LPI+FVVENN WAIGM+H R++S PEI KK  AFG+PG+ VDGMDVL VR+VA++A+ RA
Sbjct: 188 LPIIFVVENNQWAIGMAHHRSSSIPEIHKKAEAFGLPGIEVDGMDVLAVRQVAEKAVERA 247

Query: 307 RRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKLANEQEL 366
           R+G+GPTL+E  TYRFRGHSLADPDELR   EK  + ARDPI  LKK++L+N++A+  EL
Sbjct: 248 RQGQGPTLIEALTYRFRGHSLADPDELRSRQEKEAWVARDPIKKLKKHILDNQIASSDEL 307

Query: 367 KAIKDKIVEVVEEAVQFADESPHPARSQLLENVFAD 403
             I+  +   +E++V+FA  SP P  S+L   +FAD
Sbjct: 308 NDIQSSVKIDLEQSVEFAMSSPEPNISELKRYLFAD 343

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BLAST of Cucsat.G1469.T1 vs. ExPASy Swiss-Prot
Match: Q92IS3 (Pyruvate dehydrogenase E1 component subunit alpha OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=pdhA PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 2.3e-75
Identity = 134/336 (39.88%), Postives = 209/336 (62.20%), Query Frame = 0


Query: 65  VKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVST 124
           +K  K KP      TKEE +  ++DM+L R FE+ C Q+Y  G++ GF HLY GQEAV +
Sbjct: 3   IKLGKYKP------TKEEYIKSFKDMLLLRRFEEKCGQLYGMGEIGGFCHLYIGQEAVIS 62

Query: 125 GFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNL 184
               +  + D+ +++YRDH H +  G   + V++EL G+ TGC +G+GGSMH+F+  +  
Sbjct: 63  AIDMVKQKGDSTITSYRDHAHIILAGTEPKYVLAELMGRATGCSKGKGGSMHLFNVPNKF 122

Query: 185 IGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAAL 244
            GG   +G  +P+ TG AF  KY        D  ++   F GDG  N GQ +E  NMAAL
Sbjct: 123 YGGHGIVGAQVPIGTGLAFVEKYN-------DTHNICFTFLGDGAVNQGQVYEAFNMAAL 182

Query: 245 WKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIG 304
           W LP+V+++ENN +++G S  R+T   +++KKG +FG+ G  +DGMD  ++ + +K+A  
Sbjct: 183 WGLPVVYIIENNEYSMGTSVARSTFMRDLYKKGASFGIKGFQLDGMDFEEMYDGSKQAAE 242

Query: 305 RARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKLANEQ 364
             R    P ++E +TYR+RGHS++DP + R  +E  +Y  RDP+  ++K +L+NK   E 
Sbjct: 243 YVRENSFPLILEVKTYRYRGHSMSDPAKYRSKEEVEQYKERDPLVIIRKTILDNKYVTEA 302

Query: 365 ELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVF 401
           +LKAI+  + E+V+EAV+F++ SP P   +L   V+
Sbjct: 303 DLKAIEQSVKEIVKEAVEFSENSPLPDEGELYTQVY 325

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BLAST of Cucsat.G1469.T1 vs. NCBI nr
Match: XP_004140530.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis sativus] >KGN46471.1 hypothetical protein Csa_005756 [Cucumis sativus])

HSP 1 Score: 867 bits (2241), Expect = 0.0
Identity = 428/428 (100.00%), Postives = 428/428 (100.00%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469.T1 vs. NCBI nr
Match: XP_008459830.1 (PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis melo] >KAA0039805.1 pyruvate dehydrogenase E1 component subunit alpha-3 [Cucumis melo var. makuwa] >TYK24692.1 pyruvate dehydrogenase E1 component subunit alpha-3 [Cucumis melo var. makuwa])

HSP 1 Score: 855 bits (2208), Expect = 8.26e-313
Identity = 420/428 (98.13%), Postives = 425/428 (99.30%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTQRDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKY+LENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ANEQELKAIKDKIVEVVE+AVQFADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469.T1 vs. NCBI nr
Match: XP_038875269.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Benincasa hispida])

HSP 1 Score: 838 bits (2165), Expect = 2.96e-306
Identity = 411/428 (96.03%), Postives = 420/428 (98.13%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFSSSLKILQPLPLNSTRSN+KPL FDPFRS+SKFLGSRFR+PSLSKSNTR RSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDM+LGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPI ALKKY+LEN L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELK IKDKIVEVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469.T1 vs. NCBI nr
Match: XP_022992260.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita maxima])

HSP 1 Score: 812 bits (2097), Expect = 6.84e-296
Identity = 398/428 (92.99%), Postives = 412/428 (96.26%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFS+S KILQPL LNS RSN+KPL FDPFRSTS FLGSRFR PS+SKS TRCRSSPVVA
Sbjct: 1   MSFSASPKILQPLLLNSPRSNEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLV+YEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVVYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKE DC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEGDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKY++E  L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELKAI+ +I EVVEEAV+FADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIGEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469.T1 vs. NCBI nr
Match: XP_022929856.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita moschata])

HSP 1 Score: 810 bits (2092), Expect = 3.95e-295
Identity = 398/428 (92.99%), Postives = 411/428 (96.03%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MS S+S KILQPL LNS RS +KPL FDPFRSTS FLGSRFR PS+SKS TRCRSSPVVA
Sbjct: 1   MSLSASPKILQPLLLNSPRSTEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLVLYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVLYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKY++E  L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELKAI+ +I EVVEEAV+FADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIDEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469.T1 vs. ExPASy TrEMBL
Match: A0A0A0KFF3 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_6G095870 PE=4 SV=1)

HSP 1 Score: 867 bits (2241), Expect = 0.0
Identity = 428/428 (100.00%), Postives = 428/428 (100.00%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469.T1 vs. ExPASy TrEMBL
Match: A0A5A7TEC1 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002450 PE=4 SV=1)

HSP 1 Score: 855 bits (2208), Expect = 4.00e-313
Identity = 420/428 (98.13%), Postives = 425/428 (99.30%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTQRDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKY+LENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ANEQELKAIKDKIVEVVE+AVQFADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469.T1 vs. ExPASy TrEMBL
Match: A0A1S3CB66 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC103498837 PE=4 SV=1)

HSP 1 Score: 855 bits (2208), Expect = 4.00e-313
Identity = 420/428 (98.13%), Postives = 425/428 (99.30%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTQRDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKY+LENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ANEQELKAIKDKIVEVVE+AVQFADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469.T1 vs. ExPASy TrEMBL
Match: A0A6J1JV75 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111488634 PE=4 SV=1)

HSP 1 Score: 812 bits (2097), Expect = 3.31e-296
Identity = 398/428 (92.99%), Postives = 412/428 (96.26%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFS+S KILQPL LNS RSN+KPL FDPFRSTS FLGSRFR PS+SKS TRCRSSPVVA
Sbjct: 1   MSFSASPKILQPLLLNSPRSNEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLV+YEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVVYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKE DC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEGDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKY++E  L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELKAI+ +I EVVEEAV+FADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIGEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469.T1 vs. ExPASy TrEMBL
Match: A0A6J1EPB1 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111436336 PE=4 SV=1)

HSP 1 Score: 810 bits (2092), Expect = 1.91e-295
Identity = 398/428 (92.99%), Postives = 411/428 (96.03%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MS S+S KILQPL LNS RS +KPL FDPFRSTS FLGSRFR PS+SKS TRCRSSPVVA
Sbjct: 1   MSLSASPKILQPLLLNSPRSTEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLVLYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVLYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKY++E  L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELKAI+ +I EVVEEAV+FADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIDEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469.T1 vs. TAIR 10
Match: AT1G01090.1 (pyruvate dehydrogenase E1 alpha )

HSP 1 Score: 677.9 bits (1748), Expect = 5.2e-195
Identity = 340/423 (80.38%), Postives = 372/423 (87.94%), Query Frame = 0


Query: 8   KILQPLPLNSTRSNDKPLLFDPFRSTSKFLGS--RFRLPSLSKSNTRCRSSPVVAVSDVV 67
           K+   +PL+ +  N + LL       S FLGS     L  L+ SN   R SPVV+V +VV
Sbjct: 9   KLTATVPLHGSHEN-RLLLPIRLAPPSSFLGSTRSLSLRRLNHSNA-TRRSPVVSVQEVV 68

Query: 68  KEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTG 127
           KEK+   +++LLITKEEGL LYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTG
Sbjct: 69  KEKQSTNNTSLLITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVSTG 128

Query: 128 FIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLI 187
           FIKLLT+ D+VVSTYRDHVHALSKGV +R VMSELFGK TGCCRGQGGSMHMFSKEHN++
Sbjct: 129 FIKLLTKSDSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHNML 188

Query: 188 GGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALW 247
           GGFAFIGEGIPVATGAAF+SKY+REVLK+ DC DVT+AFFGDGTCNNGQFFECLNMAAL+
Sbjct: 189 GGFAFIGEGIPVATGAAFSSKYRREVLKQ-DCDDVTVAFFGDGTCNNGQFFECLNMAALY 248

Query: 248 KLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGR 307
           KLPI+FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEA+ R
Sbjct: 249 KLPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAVTR 308

Query: 308 ARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKLANEQE 367
           ARRGEGPTLVECETYRFRGHSLADPDELRD  EKA+YAARDPIAALKKY++ENKLA E E
Sbjct: 309 ARRGEGPTLVECETYRFRGHSLADPDELRDAAEKAKYAARDPIAALKKYLIENKLAKEAE 368

Query: 368 LKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTEGT 427
           LK+I+ KI E+VEEAV+FAD SP P RSQLLENVFADPKGFGIGPDG+YRCEDPKFTEGT
Sbjct: 369 LKSIEKKIDELVEEAVEFADASPQPGRSQLLENVFADPKGFGIGPDGRYRCEDPKFTEGT 428

Query: 428 AHV 429
           A V
Sbjct: 429 AQV 428

[Close]

BLAST of Cucsat.G1469.T1 vs. TAIR 10
Match: AT1G59900.1 (pyruvate dehydrogenase complex E1 alpha subunit )

HSP 1 Score: 251.5 bits (641), Expect = 1.2e-66
Identity = 140/348 (40.23%), Postives = 201/348 (57.76%), Query Frame = 0


Query: 72  PSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMF-GFVHLYNGQEAVSTGFIKLL 131
           PS ++  + +E L  +  M L R  E + A   Y+ K+  GF HLY+GQEAV+ G    +
Sbjct: 48  PSRSVESSSQELLDFFRTMALMRRME-IAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAI 107

Query: 132 TQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAF 191
           T++D +++ YRDH   L +G    EV SEL G+  GC +G+GGSMH + KE +  GG   
Sbjct: 108 TKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGI 167

Query: 192 IGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWKLPIV 251
           +G  +P+  G AF  KY +E       + VT A +GDG  N GQ FE LN++ALW LP +
Sbjct: 168 VGAQVPLGCGIAFAQKYNKE-------EAVTFALYGDGAANQGQLFEALNISALWDLPAI 227

Query: 252 FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGE 311
            V ENN + +G +  RA   P  +K+G    +PG+ VDGMD   V++  K A   A   +
Sbjct: 228 LVCENNHYGMGTAEWRAAKSPSYYKRGDY--VPGLKVDGMDAFAVKQACKFAKQHALE-K 287

Query: 312 GPTLVECETYRFRGHSLADP-DELRDPDE-KARYAARDPIAALKKYMLENKLANEQELKA 371
           GP ++E +TYR+ GHS++DP    R  DE       RDPI  +KK +L + LA E+ELK 
Sbjct: 288 GPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKD 347

Query: 372 IKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFG---IGPDGK 414
           ++ +I + V++A+  A + P P  S+L  NV+   KGFG    GPD K
Sbjct: 348 MEKEIRKEVDDAIAKAKDCPMPEPSELFTNVYV--KGFGTESFGPDRK 382

[Close]

BLAST of Cucsat.G1469.T1 vs. TAIR 10
Match: AT1G24180.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.5e-64
Identity = 141/377 (37.40%), Postives = 211/377 (55.97%), Query Frame = 0


Query: 43  LPSLSKSNTRCRSSPVVAVSDVVKEKKL--KPSSNLLITKEEGLVLYEDMILGREFEDMC 102
           LPS  + +    SSP+   + V     L   PS ++  + EE L  + DM   R  E + 
Sbjct: 21  LPSSIRRHVSTDSSPITIETAVPFTSHLCESPSRSVETSSEEILAFFRDMARMRRME-IA 80

Query: 103 AQMYYRGKMF-GFVHLYNGQEAVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSE 162
           A   Y+ K+  GF HLY+GQEA++ G    +T++D ++++YRDH   + +G    +  SE
Sbjct: 81  ADSLYKAKLIRGFCHLYDGQEALAVGMEAAITKKDAIITSYRDHCTFIGRGGKLVDAFSE 140

Query: 163 LFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQD 222
           L G+ TGC  G+GGSMH + K+ +  GG   +G  IP+  G AF  KY ++       + 
Sbjct: 141 LMGRKTGCSHGKGGSMHFYKKDASFYGGHGIVGAQIPLGCGLAFAQKYNKD-------EA 200

Query: 223 VTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPA 282
           VT A +GDG  N GQ FE LN++ALW LP + V ENN + +G +  R+   P  +K+G  
Sbjct: 201 VTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTATWRSAKSPAYFKRGDY 260

Query: 283 FGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVECETYRFRGHSLADP-DELRDPDE 342
             +PG+ VDGMD L V++  K A   A +  GP ++E +TYR+ GHS++DP    R  DE
Sbjct: 261 --VPGLKVDGMDALAVKQACKFAKEHALK-NGPIILEMDTYRYHGHSMSDPGSTYRTRDE 320

Query: 343 -KARYAARDPIAALKKYMLENKLANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLE 402
                  RDPI  ++K +L + +A E+ELK ++ +I + V++AV  A ESP P  S+L  
Sbjct: 321 ISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIRKEVDDAVAQAKESPIPDASELFT 380

Query: 403 NVFADPKGF-GIGPDGK 414
           N++    G    G D K
Sbjct: 381 NMYVKDCGVESFGADRK 386

[Close]

BLAST of Cucsat.G1469.T1 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 8.4e-28
Identity = 86/327 (26.30%), Postives = 160/327 (48.93%), Query Frame = 0


Query: 78  ITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTQRDTVV 137
           +++E  + +Y DM+  +  +++  +   +G++  F     G+EA++      LT +D + 
Sbjct: 125 VSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRL-SFYATAIGEEAINIASAAALTPQDVIF 184

Query: 138 STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 197
             YR+    L +G   +E  ++ FG  +   +G+   +H  S + N     A I   +P 
Sbjct: 185 PQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPN 244

Query: 198 ATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 257
           A GAA++ K  +   K+A      + +FGDG  + G F   LN+AA+ + P++F+  NN 
Sbjct: 245 AVGAAYSLKMDK---KDA----CAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNG 304

Query: 258 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 317
           WAI            +  KG A+G+  + VDG D L +      A   A R + P L+E 
Sbjct: 305 WAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEA 364

Query: 318 ETYRFRGHSLADPD-ELRDPDEKARY-AARDPIAALKKYMLENKLANEQELKAIKDKIVE 377
            TYR   HS +D     R   E   +  AR+P++  + ++  N   +++    ++ +I +
Sbjct: 365 LTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKK 424

Query: 378 VVEEAVQFADESPHPARSQLLENVFAD 403
            + EA++ A+++  P     L+N+F+D
Sbjct: 425 EMLEALRVAEKTEKPN----LQNMFSD 439

[Close]

BLAST of Cucsat.G1469.T1 vs. TAIR 10
Match: AT5G09300.2 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 8.4e-28
Identity = 86/327 (26.30%), Postives = 160/327 (48.93%), Query Frame = 0


Query: 78  ITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTQRDTVV 137
           +++E  + +Y DM+  +  +++  +   +G++  F     G+EA++      LT +D + 
Sbjct: 54  VSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRL-SFYATAIGEEAINIASAAALTPQDVIF 113

Query: 138 STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 197
             YR+    L +G   +E  ++ FG  +   +G+   +H  S + N     A I   +P 
Sbjct: 114 PQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPN 173

Query: 198 ATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 257
           A GAA++ K  +   K+A      + +FGDG  + G F   LN+AA+ + P++F+  NN 
Sbjct: 174 AVGAAYSLKMDK---KDA----CAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNG 233

Query: 258 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 317
           WAI            +  KG A+G+  + VDG D L +      A   A R + P L+E 
Sbjct: 234 WAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEA 293

Query: 318 ETYRFRGHSLADPD-ELRDPDEKARY-AARDPIAALKKYMLENKLANEQELKAIKDKIVE 377
            TYR   HS +D     R   E   +  AR+P++  + ++  N   +++    ++ +I +
Sbjct: 294 LTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKK 353

Query: 378 VVEEAVQFADESPHPARSQLLENVFAD 403
            + EA++ A+++  P     L+N+F+D
Sbjct: 354 EMLEALRVAEKTEKPN----LQNMFSD 368

The following BLAST results are available for this feature:

BLAST of Cucsat.G1469.T1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24457	7.3e-194	80.38	Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Arabidopsi...	[more]
Q7XTJ3	2.3e-179	74.25	Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Oryza sati...	[more]
Q1XDM0	9.7e-130	68.18	Pyruvate dehydrogenase E1 component subunit alpha OS=Pyropia yezoensis OX=2788 G...	[more]
P51267	4.1e-128	66.67	Pyruvate dehydrogenase E1 component subunit alpha OS=Porphyra purpurea OX=2787 G...	[more]
Q92IS3	2.3e-75	39.88	Pyruvate dehydrogenase E1 component subunit alpha OS=Rickettsia conorii (strain ...	[more]

BLAST of Cucsat.G1469.T1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140530.1	0.0	100.00	pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis sati...	[more]
XP_008459830.1	8.26e-313	98.13	PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [C...	[more]
XP_038875269.1	2.96e-306	96.03	pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Benincasa hi...	[more]
XP_022992260.1	6.84e-296	92.99	pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita ma...	[more]
XP_022929856.1	3.95e-295	92.99	pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita mo...	[more]

BLAST of Cucsat.G1469.T1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KFF3	0.0	100.00	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=...	[more]
A0A5A7TEC1	4.00e-313	98.13	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX...	[more]
A0A1S3CB66	4.00e-313	98.13	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A6J1JV75	3.31e-296	92.99	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1EPB1	1.91e-295	92.99	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 ...	[more]

BLAST of Cucsat.G1469.T1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G01090.1	5.2e-195	80.38	pyruvate dehydrogenase E1 alpha	[more]
AT1G59900.1	1.2e-66	40.23	pyruvate dehydrogenase complex E1 alpha subunit	[more]
AT1G24180.1	2.5e-64	37.40	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G09300.1	8.4e-28	26.30	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G09300.2	8.4e-28	26.30	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001017	Dehydrogenase, E1 component	PFAM	PF00676	E1_dh	coord: 89..390 e-value: 1.2E-80 score: 270.6
None	No IPR available	GENE3D	3.40.50.970		coord: 46..410 e-value: 4.1E-122 score: 409.7
None	No IPR available	PANTHER	PTHR11516	PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR	coord: 5..428
None	No IPR available	PANTHER	PTHR11516:SF58	PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA	coord: 5..428
None	No IPR available	CDD	cd02000	TPP_E1_PDC_ADC_BCADC	coord: 86..384 e-value: 1.16639E-152 score: 432.305
IPR017597	Pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit, subgroup y	TIGRFAM	TIGR03182	TIGR03182	coord: 81..401 e-value: 3.5E-140 score: 464.3
IPR029061	Thiamin diphosphate-binding fold	SUPERFAMILY	52518	Thiamin diphosphate-binding fold (THDP-binding)	coord: 75..403

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G1469	Cucsat.G1469	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G1469.T1.E1	Cucsat.G1469.T1.E1	exon
Cucsat.G1469.T1.E2	Cucsat.G1469.T1.E2	exon
Cucsat.G1469.T1.E3	Cucsat.G1469.T1.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G1469.T1.C3	Cucsat.G1469.T1.C3	CDS
Cucsat.G1469.T1.C2	Cucsat.G1469.T1.C2	CDS
Cucsat.G1469.T1.C1	Cucsat.G1469.T1.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G1469.T1	Cucsat.G1469.T1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006086	acetyl-CoA biosynthetic process from pyruvate
biological_process	GO:0006096	glycolytic process
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016624	oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
molecular_function	GO:0004739	pyruvate dehydrogenase (acetyl-transferring) activity