Cucsat.G1469 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G1469
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Pyruvate dehydrogenase E1 component subunit alpha
Location	ctg10: 149975 .. 153036 (-)
RNA-Seq Expression	Cucsat.G1469
Synteny	Cucsat.G1469

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTGATTTGGTTTGAGAGTAAAATTCTCATAAATACACACACTTTAATATTTAGATAAATATGTGAAGAAAATTAGGAAGTAGTTGGTTTTAGTTGAGTGGCTTTCTTCTTTCTCCTCCGTTTCAATTTCATCTTTGTGGCTTTTGCAGTGAAAAAACGAACACCAAAATCCTCACGCCTCAGAAAAGGCGGCGATTTCCAAGCTCCTTCTCCTCGTTTCCTCCGCCCCTTTCTTCATTCTCTTCCCCTTTTTATATCTTTCTCTTCTTTTCTCTTTTCTTTCATTTTCCCATCTCTCCTTTTTCCCATTTACTCCCTTCTTCTTTCTCACACACAATGTCCTTCTCTTCATCACTCAAAATCCTCCAACCCCTTCCTCTTAACTCCACCAGATCCAATGACAAACCCCTTTTATTTGATCCCTTTAGATCCACCTCCAAGTTTCTTGGATCTCGATTTCGTCTCCCTTCTCTCTCTAAATCCAATACTCGTTGTCGATCTTCCCCTGTTGTTGCCGTTTCCGATGTTGTCAAGGAGAAGAAGCTCAAACCCAGTTCCAATTTGGTACTTTTTTTTTTTTTTTTTTTTTTTAATTCTAGATTCTGAATTTGTTGTATTGATTGATTTGTTATTTGGGTTTTTAATGTCCTGCAAATTATGGGGGGATTTGGTAATGTTGCATGACATTTTAGCTTTAACGATTTTGCTTTTGTTGGTTTTGGTTTCCCTGTTGTTATTTTCTTTTGGAAAATTGGATGGTTTGATTGAGATTGTCTTTTTGAAATTGGAAGTTATATTGTTCTTTTGTCCCATCAGTGGAATTAAAATTTTACTCTACTGAAGCTTTTGTGTGCATTGCTTTGCTGCATGAAGCATCTTGGTTTAAAAGTTCTTCACTTTTTTTGGTTCCTTTTATTGTCTCCACACAGCTGATTACAAAAGAAGAGGGTTTGGTACTCTATGAGGACATGATATTAGGCAGAGAATTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCCACTGGGTTCATCAAGCTTCTTACACAGAGGGATACTGTAGTAAGCACTTATCGTGATCACGTTCATGCTCTCAGTAAGGGAGTTCCATCTCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGGTGCTGCCGTGGCCAAGGTGGCTCAATGCATATGTTCTCAAAAGAACATAATCTGATTGGGGGATTTGCCTTCATTGGAGAAGGCATACCAGTGGCCACTGGTGCTGCATTCACGTCTAAATACAAGAGAGAAGTTTTAAAAGAGGCAGACTGTCAAGATGTGACATTGGCATTTTTCGGGGATGGAACTTGCAACAATGGCCAGTTCTTCGAGTGCTTGAACATGGCAGCTCTGTGGAAATTGCCAATCGTATTTGTTGTGGAGAATAATCTTTGGGCAATTGGAATGTCGCATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAAAAAGGTCCTGCATTTGGAATGCCTGGTGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGAGAGGTTGCAAAGGAGGCAATTGGAAGAGCCAGAAGAGGAGAAGGACCGACTCTCGTGGAATGTGAAACCTATAGGTTTAGAGGACACTCTCTGGCTGATCCAGATGAACTCCGTGACCCAGGTTAGTGCTCTTAAATCCATACCTACAATTGGGTCTGTCATATTCAAAATATGCCTTTGCTTGGCCTTCTTGACATTTATTATTACGGCTAGTCAAAGTTCCTTAATTCATTTCTCGTGAATATGATAGCTGATCTCTTAATTCGTACTCCATCATAGCCATTTAAGGATAATTCATTCAGGGTTGTTAATGAGCTGCACCGCTTGCAATTCACTCATTAGTTAACTCGAAATTTTTTCTCATTTGTTAATTAGATTTTTTGTTTTTCTTTATTTTTTAAATGGAATCAACTAACTTTGATTGAAAAAAAAAATGTAAGAATACAAGGACATACGTCCACCAAAGCACCCCTTCTAAAGAAAGGGCTTCCACAACTGAGTAAGATATTACTTAGAGAATAATTACAAAAGAAAAAAAAGCTTTGAAACTGAAGCACGAAGCGAGACATGAAGACTCACGAGGAATCAAATCTCACTCGTGTTCCTGTTCAAACCTCTAAACACACTAGTGTTCCTCTTCCCTCGAATATCCCACAATAAAGCTGATTCTTCATAGATGATAGAAATTGATGTCCCCATCAGGTTCTTCATTGTACCTTACACGCACAAATGTAGTAGTTTCTTATCTAAGTATAGTAACTCCGGAAAGTACAAAGGATTAAGAATTCCCTGATTTAACCATTGCAGATGAGAAGGCTCGTTATGCTGCCAGAGATCCCATTGCAGCTTTGAAGAAGTACATGTTGGAGAATAAACTAGCCAATGAACAGGAGCTAAAGGCAATAAAGGATAAGATAGTTGAGGTGGTTGAGGAGGCAGTTCAATTTGCAGATGAAAGCCCCCATCCAGCAAGAAGCCAGTTATTAGAGAATGTCTTTGCCGATCCTAAAGGCTTTGGAATTGGGCCTGATGGAAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGAACTGCACATGTCTAAACTTCACGAACTTTGTTGTCTATCTAAGTCTTTTTATTTATTTTTATTTGTTTTCTTTACTGTTCCTGTTGGGTTATTTTCTGATTATTTGTAATTGAATGTTAAATTAGCTTTTTTCTGAAGCAACTAGGAATTTCCTTCCTTTCTTCAGGTTGTGCTGTAGTTTTTTTTTTCTTTCTTTTTCTTCTTTTTCTTTTTACCAATTTTGATTTAGTGTATGATACAAACATTCCTCTACTAATCTAGTTATGTGGGAGATGGAAGCATTGTTTAATGCTTTACATATTATGATCATGAGCAGTTTAAACGCTATCGTAGTTTTGGAACTTTTATACTTCATACTTCATATTCTGTTTTCATCCTTCTACTCTGGTTTTTGCTCCTTTTG

Coding sequence (CDS)

ATGTCCTTCTCTTCATCACTCAAAATCCTCCAACCCCTTCCTCTTAACTCCACCAGATCCAATGACAAACCCCTTTTATTTGATCCCTTTAGATCCACCTCCAAGTTTCTTGGATCTCGATTTCGTCTCCCTTCTCTCTCTAAATCCAATACTCGTTGTCGATCTTCCCCTGTTGTTGCCGTTTCCGATGTTGTCAAGGAGAAGAAGCTCAAACCCAGTTCCAATTTGCTGATTACAAAAGAAGAGGGTTTGGTACTCTATGAGGACATGATATTAGGCAGAGAATTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCCACTGGGTTCATCAAGCTTCTTACACAGAGGGATACTGTAGTAAGCACTTATCGTGATCACGTTCATGCTCTCAGTAAGGGAGTTCCATCTCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGGTGCTGCCGTGGCCAAGGTGGCTCAATGCATATGTTCTCAAAAGAACATAATCTGATTGGGGGATTTGCCTTCATTGGAGAAGGCATACCAGTGGCCACTGGTGCTGCATTCACGTCTAAATACAAGAGAGAAGTTTTAAAAGAGGCAGACTGTCAAGATGTGACATTGGCATTTTTCGGGGATGGAACTTGCAACAATGGCCAGTTCTTCGAGTGCTTGAACATGGCAGCTCTGTGGAAATTGCCAATCGTATTTGTTGTGGAGAATAATCTTTGGGCAATTGGAATGTCGCATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAAAAAGGTCCTGCATTTGGAATGCCTGGTGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGAGAGGTTGCAAAGGAGGCAATTGGAAGAGCCAGAAGAGGAGAAGGACCGACTCTCGTGGAATGTGAAACCTATAGGTTTAGAGGACACTCTCTGGCTGATCCAGATGAACTCCGTGACCCAGATGAGAAGGCTCGTTATGCTGCCAGAGATCCCATTGCAGCTTTGAAGAAGTACATGTTGGAGAATAAACTAGCCAATGAACAGGAGCTAAAGGCAATAAAGGATAAGATAGTTGAGGTGGTTGAGGAGGCAGTTCAATTTGCAGATGAAAGCCCCCATCCAGCAAGAAGCCAGTTATTAGAGAATGTCTTTGCCGATCCTAAAGGCTTTGGAATTGGGCCTGATGGAAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGAACTGCACATGTCTAA

Protein sequence

MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVAVSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKLANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTEGTAHV

Homology

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BLAST of Cucsat.G1469 vs. ExPASy Swiss-Prot
Match: O24457 (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PDH-E1 ALPHA PE=2 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 7.3e-194
Identity = 340/423 (80.38%), Postives = 372/423 (87.94%), Query Frame = 0


Query: 8   KILQPLPLNSTRSNDKPLLFDPFRSTSKFLGS--RFRLPSLSKSNTRCRSSPVVAVSDVV 67
           K+   +PL+ +  N + LL       S FLGS     L  L+ SN   R SPVV+V +VV
Sbjct: 9   KLTATVPLHGSHEN-RLLLPIRLAPPSSFLGSTRSLSLRRLNHSNA-TRRSPVVSVQEVV 68

Query: 68  KEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTG 127
           KEK+   +++LLITKEEGL LYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTG
Sbjct: 69  KEKQSTNNTSLLITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVSTG 128

Query: 128 FIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLI 187
           FIKLLT+ D+VVSTYRDHVHALSKGV +R VMSELFGK TGCCRGQGGSMHMFSKEHN++
Sbjct: 129 FIKLLTKSDSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHNML 188

Query: 188 GGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALW 247
           GGFAFIGEGIPVATGAAF+SKY+REVLK+ DC DVT+AFFGDGTCNNGQFFECLNMAAL+
Sbjct: 189 GGFAFIGEGIPVATGAAFSSKYRREVLKQ-DCDDVTVAFFGDGTCNNGQFFECLNMAALY 248

Query: 248 KLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGR 307
           KLPI+FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEA+ R
Sbjct: 249 KLPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAVTR 308

Query: 308 ARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKLANEQE 367
           ARRGEGPTLVECETYRFRGHSLADPDELRD  EKA+YAARDPIAALKKY++ENKLA E E
Sbjct: 309 ARRGEGPTLVECETYRFRGHSLADPDELRDAAEKAKYAARDPIAALKKYLIENKLAKEAE 368

Query: 368 LKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTEGT 427
           LK+I+ KI E+VEEAV+FAD SP P RSQLLENVFADPKGFGIGPDG+YRCEDPKFTEGT
Sbjct: 369 LKSIEKKIDELVEEAVEFADASPQPGRSQLLENVFADPKGFGIGPDGRYRCEDPKFTEGT 428

Query: 428 AHV 429
           A V
Sbjct: 429 AQV 428

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BLAST of Cucsat.G1469 vs. ExPASy Swiss-Prot
Match: Q7XTJ3 (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0119400 PE=2 SV=2)

HSP 1 Score: 629.8 bits (1623), Expect = 2.3e-179
Identity = 320/431 (74.25%), Postives = 355/431 (82.37%), Query Frame = 0


Query: 2   SFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVAV 61
           SF+++ K L P+   S      PL   P  +++     R       K   R R++  V+ 
Sbjct: 6   SFTAAAKFLAPVSARSAGDYKPPL---PLPASASLRPGR-------KPAPRLRTALAVS- 65

Query: 62  SDVVKEKKLKP--SSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQ 121
           SDV+   K  P  +++  +T+EE L LYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQ
Sbjct: 66  SDVLPGNKAAPAAAAHSAVTREEALELYEDMVLGRIFEDMCAQMYYRGKMFGFVHLYNGQ 125

Query: 122 EAVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFS 181
           EAVSTGFIKLL Q D VVSTYRDHVHALSKGVP+R VM+ELFGK TGCCRGQGGSMHMFS
Sbjct: 126 EAVSTGFIKLLNQADCVVSTYRDHVHALSKGVPARSVMAELFGKATGCCRGQGGSMHMFS 185

Query: 182 KEHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEA--DCQDVTLAFFGDGTCNNGQFFE 241
           + HNL+GGFAFIGEGIPVATGAAF +KY+ EVLK++  D  DVTLAFFGDGTCNNGQFFE
Sbjct: 186 EPHNLLGGFAFIGEGIPVATGAAFAAKYRHEVLKQSSPDGLDVTLAFFGDGTCNNGQFFE 245

Query: 242 CLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVRE 301
           CLNMA LWKLPIVFVVENNLWAIGMSHLRATSDPEI+KKGPAFGMPGVHVDGMDVLKVRE
Sbjct: 246 CLNMAQLWKLPIVFVVENNLWAIGMSHLRATSDPEIYKKGPAFGMPGVHVDGMDVLKVRE 305

Query: 302 VAKEAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLE 361
           VAKEAI RARRGEGPTLVECETYRFRGHSLADPDELR PDEK+ YAARDPI ALKKY++E
Sbjct: 306 VAKEAIERARRGEGPTLVECETYRFRGHSLADPDELRRPDEKSHYAARDPITALKKYIIE 365

Query: 362 NKLANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCE 421
             LA E ELK+I+ KI +VVEEAV+FAD SP P RSQLLENVF+DPKGFGIGPDGKYRCE
Sbjct: 366 QNLATESELKSIEKKIDDVVEEAVEFADASPLPPRSQLLENVFSDPKGFGIGPDGKYRCE 425

Query: 422 DPKFTEGTAHV 429
           DP FT+GTA V
Sbjct: 426 DPLFTQGTAQV 425

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BLAST of Cucsat.G1469 vs. ExPASy Swiss-Prot
Match: Q1XDM0 (Pyruvate dehydrogenase E1 component subunit alpha OS=Pyropia yezoensis OX=2788 GN=pdhA PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 9.7e-130
Identity = 225/330 (68.18%), Postives = 264/330 (80.00%), Query Frame = 0


Query: 73  SSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTQ 132
           S+ L + K   LVLYEDM+LGR FEDMCAQMYY+GKMFGFVHLYNGQEAVSTG IKLL  
Sbjct: 16  STGLNLNKSNLLVLYEDMLLGRNFEDMCAQMYYKGKMFGFVHLYNGQEAVSTGVIKLLNP 75

Query: 133 RDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIG 192
            D V STYRDHVHALSKGVPS+ VM+ELFGK TGC +G+GGSMH+FS  HN +GGFAFI 
Sbjct: 76  TDYVCSTYRDHVHALSKGVPSKNVMAELFGKETGCSKGRGGSMHIFSAPHNFLGGFAFIA 135

Query: 193 EGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFV 252
           EGIPVATGAAF S Y+++VLKE +   VT  FFGDGT NNGQFFECLNMA LWKLPI+FV
Sbjct: 136 EGIPVATGAAFQSIYRQQVLKETEDLRVTACFFGDGTTNNGQFFECLNMAVLWKLPIIFV 195

Query: 253 VENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGP 312
           VENN WAIGM+H R++S PEI KK  AFG+PG+ VDGMDVL VR+ AK+A+ RAR+G+GP
Sbjct: 196 VENNQWAIGMAHHRSSSIPEIHKKAEAFGLPGIEVDGMDVLAVRQAAKQAVQRARQGDGP 255

Query: 313 TLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKLANEQELKAIKDK 372
           TL+E  TYRFRGHSLADPDELR   EK  + ARDPI  LKKY+L+N++AN  EL  I++ 
Sbjct: 256 TLIEALTYRFRGHSLADPDELRSRQEKEAWVARDPIKKLKKYILDNEIANIGELNEIQNA 315

Query: 373 IVEVVEEAVQFADESPHPARSQLLENVFAD 403
           +   +E+AV+FA  SP P  S+L   +FAD
Sbjct: 316 VKTELEQAVKFAISSPEPNMSELKRYLFAD 345

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BLAST of Cucsat.G1469 vs. ExPASy Swiss-Prot
Match: P51267 (Pyruvate dehydrogenase E1 component subunit alpha OS=Porphyra purpurea OX=2787 GN=pdhA PE=3 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 4.1e-128
Identity = 224/336 (66.67%), Postives = 267/336 (79.46%), Query Frame = 0


Query: 67  EKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGF 126
           E  L   + + +TK + LVLYEDM+LGR FEDMCAQMYY+GKMFGFVHLYNGQEAVSTG 
Sbjct: 8   ELPLTNCNQINLTKHKLLVLYEDMLLGRNFEDMCAQMYYKGKMFGFVHLYNGQEAVSTGV 67

Query: 127 IKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIG 186
           IKLL  +D V STYRDHVHALSKGVPS+ VM+ELFGK TGC RG+GGSMH+FS  HN +G
Sbjct: 68  IKLLDSKDYVCSTYRDHVHALSKGVPSQNVMAELFGKETGCSRGRGGSMHIFSAPHNFLG 127

Query: 187 GFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWK 246
           GFAFI EGIPVATGAAF S Y+++VLKE     VT  FFGDGT NNGQFFECLNMA LWK
Sbjct: 128 GFAFIAEGIPVATGAAFQSIYRQQVLKEPGELRVTACFFGDGTTNNGQFFECLNMAVLWK 187

Query: 247 LPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRA 306
           LPI+FVVENN WAIGM+H R++S PEI KK  AFG+PG+ VDGMDVL VR+VA++A+ RA
Sbjct: 188 LPIIFVVENNQWAIGMAHHRSSSIPEIHKKAEAFGLPGIEVDGMDVLAVRQVAEKAVERA 247

Query: 307 RRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKLANEQEL 366
           R+G+GPTL+E  TYRFRGHSLADPDELR   EK  + ARDPI  LKK++L+N++A+  EL
Sbjct: 248 RQGQGPTLIEALTYRFRGHSLADPDELRSRQEKEAWVARDPIKKLKKHILDNQIASSDEL 307

Query: 367 KAIKDKIVEVVEEAVQFADESPHPARSQLLENVFAD 403
             I+  +   +E++V+FA  SP P  S+L   +FAD
Sbjct: 308 NDIQSSVKIDLEQSVEFAMSSPEPNISELKRYLFAD 343

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BLAST of Cucsat.G1469 vs. ExPASy Swiss-Prot
Match: Q92IS3 (Pyruvate dehydrogenase E1 component subunit alpha OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=pdhA PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 2.3e-75
Identity = 134/336 (39.88%), Postives = 209/336 (62.20%), Query Frame = 0


Query: 65  VKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVST 124
           +K  K KP      TKEE +  ++DM+L R FE+ C Q+Y  G++ GF HLY GQEAV +
Sbjct: 3   IKLGKYKP------TKEEYIKSFKDMLLLRRFEEKCGQLYGMGEIGGFCHLYIGQEAVIS 62

Query: 125 GFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNL 184
               +  + D+ +++YRDH H +  G   + V++EL G+ TGC +G+GGSMH+F+  +  
Sbjct: 63  AIDMVKQKGDSTITSYRDHAHIILAGTEPKYVLAELMGRATGCSKGKGGSMHLFNVPNKF 122

Query: 185 IGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAAL 244
            GG   +G  +P+ TG AF  KY        D  ++   F GDG  N GQ +E  NMAAL
Sbjct: 123 YGGHGIVGAQVPIGTGLAFVEKYN-------DTHNICFTFLGDGAVNQGQVYEAFNMAAL 182

Query: 245 WKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIG 304
           W LP+V+++ENN +++G S  R+T   +++KKG +FG+ G  +DGMD  ++ + +K+A  
Sbjct: 183 WGLPVVYIIENNEYSMGTSVARSTFMRDLYKKGASFGIKGFQLDGMDFEEMYDGSKQAAE 242

Query: 305 RARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKLANEQ 364
             R    P ++E +TYR+RGHS++DP + R  +E  +Y  RDP+  ++K +L+NK   E 
Sbjct: 243 YVRENSFPLILEVKTYRYRGHSMSDPAKYRSKEEVEQYKERDPLVIIRKTILDNKYVTEA 302

Query: 365 ELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVF 401
           +LKAI+  + E+V+EAV+F++ SP P   +L   V+
Sbjct: 303 DLKAIEQSVKEIVKEAVEFSENSPLPDEGELYTQVY 325

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BLAST of Cucsat.G1469 vs. NCBI nr
Match: XP_004140530.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis sativus] >KGN46471.1 hypothetical protein Csa_005756 [Cucumis sativus])

HSP 1 Score: 867 bits (2241), Expect = 0.0
Identity = 428/428 (100.00%), Postives = 428/428 (100.00%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469 vs. NCBI nr
Match: XP_008459830.1 (PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis melo] >KAA0039805.1 pyruvate dehydrogenase E1 component subunit alpha-3 [Cucumis melo var. makuwa] >TYK24692.1 pyruvate dehydrogenase E1 component subunit alpha-3 [Cucumis melo var. makuwa])

HSP 1 Score: 855 bits (2208), Expect = 8.26e-313
Identity = 420/428 (98.13%), Postives = 425/428 (99.30%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTQRDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKY+LENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ANEQELKAIKDKIVEVVE+AVQFADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469 vs. NCBI nr
Match: XP_038875269.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Benincasa hispida])

HSP 1 Score: 838 bits (2165), Expect = 2.96e-306
Identity = 411/428 (96.03%), Postives = 420/428 (98.13%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFSSSLKILQPLPLNSTRSN+KPL FDPFRS+SKFLGSRFR+PSLSKSNTR RSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDM+LGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPI ALKKY+LEN L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELK IKDKIVEVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469 vs. NCBI nr
Match: XP_022992260.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita maxima])

HSP 1 Score: 812 bits (2097), Expect = 6.84e-296
Identity = 398/428 (92.99%), Postives = 412/428 (96.26%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFS+S KILQPL LNS RSN+KPL FDPFRSTS FLGSRFR PS+SKS TRCRSSPVVA
Sbjct: 1   MSFSASPKILQPLLLNSPRSNEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLV+YEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVVYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKE DC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEGDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKY++E  L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELKAI+ +I EVVEEAV+FADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIGEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469 vs. NCBI nr
Match: XP_022929856.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita moschata])

HSP 1 Score: 810 bits (2092), Expect = 3.95e-295
Identity = 398/428 (92.99%), Postives = 411/428 (96.03%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MS S+S KILQPL LNS RS +KPL FDPFRSTS FLGSRFR PS+SKS TRCRSSPVVA
Sbjct: 1   MSLSASPKILQPLLLNSPRSTEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLVLYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVLYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKY++E  L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELKAI+ +I EVVEEAV+FADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIDEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469 vs. ExPASy TrEMBL
Match: A0A0A0KFF3 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_6G095870 PE=4 SV=1)

HSP 1 Score: 867 bits (2241), Expect = 0.0
Identity = 428/428 (100.00%), Postives = 428/428 (100.00%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469 vs. ExPASy TrEMBL
Match: A0A5A7TEC1 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002450 PE=4 SV=1)

HSP 1 Score: 855 bits (2208), Expect = 4.00e-313
Identity = 420/428 (98.13%), Postives = 425/428 (99.30%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTQRDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKY+LENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ANEQELKAIKDKIVEVVE+AVQFADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469 vs. ExPASy TrEMBL
Match: A0A1S3CB66 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC103498837 PE=4 SV=1)

HSP 1 Score: 855 bits (2208), Expect = 4.00e-313
Identity = 420/428 (98.13%), Postives = 425/428 (99.30%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTQRDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKY+LENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ANEQELKAIKDKIVEVVE+AVQFADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469 vs. ExPASy TrEMBL
Match: A0A6J1JV75 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111488634 PE=4 SV=1)

HSP 1 Score: 812 bits (2097), Expect = 3.31e-296
Identity = 398/428 (92.99%), Postives = 412/428 (96.26%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MSFS+S KILQPL LNS RSN+KPL FDPFRSTS FLGSRFR PS+SKS TRCRSSPVVA
Sbjct: 1   MSFSASPKILQPLLLNSPRSNEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLV+YEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVVYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKE DC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEGDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKY++E  L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELKAI+ +I EVVEEAV+FADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIGEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469 vs. ExPASy TrEMBL
Match: A0A6J1EPB1 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111436336 PE=4 SV=1)

HSP 1 Score: 810 bits (2092), Expect = 1.91e-295
Identity = 398/428 (92.99%), Postives = 411/428 (96.03%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60
           MS S+S KILQPL LNS RS +KPL FDPFRSTS FLGSRFR PS+SKS TRCRSSPVVA
Sbjct: 1   MSLSASPKILQPLLLNSPRSTEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLVLYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVLYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKY++E  L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELKAI+ +I EVVEEAV+FADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIDEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 428
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Cucsat.G1469 vs. TAIR 10
Match: AT1G01090.1 (pyruvate dehydrogenase E1 alpha )

HSP 1 Score: 677.9 bits (1748), Expect = 5.2e-195
Identity = 340/423 (80.38%), Postives = 372/423 (87.94%), Query Frame = 0


Query: 8   KILQPLPLNSTRSNDKPLLFDPFRSTSKFLGS--RFRLPSLSKSNTRCRSSPVVAVSDVV 67
           K+   +PL+ +  N + LL       S FLGS     L  L+ SN   R SPVV+V +VV
Sbjct: 9   KLTATVPLHGSHEN-RLLLPIRLAPPSSFLGSTRSLSLRRLNHSNA-TRRSPVVSVQEVV 68

Query: 68  KEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTG 127
           KEK+   +++LLITKEEGL LYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQEAVSTG
Sbjct: 69  KEKQSTNNTSLLITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVSTG 128

Query: 128 FIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLI 187
           FIKLLT+ D+VVSTYRDHVHALSKGV +R VMSELFGK TGCCRGQGGSMHMFSKEHN++
Sbjct: 129 FIKLLTKSDSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHNML 188

Query: 188 GGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALW 247
           GGFAFIGEGIPVATGAAF+SKY+REVLK+ DC DVT+AFFGDGTCNNGQFFECLNMAAL+
Sbjct: 189 GGFAFIGEGIPVATGAAFSSKYRREVLKQ-DCDDVTVAFFGDGTCNNGQFFECLNMAALY 248

Query: 248 KLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGR 307
           KLPI+FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEA+ R
Sbjct: 249 KLPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAVTR 308

Query: 308 ARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKLANEQE 367
           ARRGEGPTLVECETYRFRGHSLADPDELRD  EKA+YAARDPIAALKKY++ENKLA E E
Sbjct: 309 ARRGEGPTLVECETYRFRGHSLADPDELRDAAEKAKYAARDPIAALKKYLIENKLAKEAE 368

Query: 368 LKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTEGT 427
           LK+I+ KI E+VEEAV+FAD SP P RSQLLENVFADPKGFGIGPDG+YRCEDPKFTEGT
Sbjct: 369 LKSIEKKIDELVEEAVEFADASPQPGRSQLLENVFADPKGFGIGPDGRYRCEDPKFTEGT 428

Query: 428 AHV 429
           A V
Sbjct: 429 AQV 428

[Close]

BLAST of Cucsat.G1469 vs. TAIR 10
Match: AT1G59900.1 (pyruvate dehydrogenase complex E1 alpha subunit )

HSP 1 Score: 251.5 bits (641), Expect = 1.2e-66
Identity = 140/348 (40.23%), Postives = 201/348 (57.76%), Query Frame = 0


Query: 72  PSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMF-GFVHLYNGQEAVSTGFIKLL 131
           PS ++  + +E L  +  M L R  E + A   Y+ K+  GF HLY+GQEAV+ G    +
Sbjct: 48  PSRSVESSSQELLDFFRTMALMRRME-IAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAI 107

Query: 132 TQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAF 191
           T++D +++ YRDH   L +G    EV SEL G+  GC +G+GGSMH + KE +  GG   
Sbjct: 108 TKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGI 167

Query: 192 IGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWKLPIV 251
           +G  +P+  G AF  KY +E       + VT A +GDG  N GQ FE LN++ALW LP +
Sbjct: 168 VGAQVPLGCGIAFAQKYNKE-------EAVTFALYGDGAANQGQLFEALNISALWDLPAI 227

Query: 252 FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGE 311
            V ENN + +G +  RA   P  +K+G    +PG+ VDGMD   V++  K A   A   +
Sbjct: 228 LVCENNHYGMGTAEWRAAKSPSYYKRGDY--VPGLKVDGMDAFAVKQACKFAKQHALE-K 287

Query: 312 GPTLVECETYRFRGHSLADP-DELRDPDE-KARYAARDPIAALKKYMLENKLANEQELKA 371
           GP ++E +TYR+ GHS++DP    R  DE       RDPI  +KK +L + LA E+ELK 
Sbjct: 288 GPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKD 347

Query: 372 IKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFG---IGPDGK 414
           ++ +I + V++A+  A + P P  S+L  NV+   KGFG    GPD K
Sbjct: 348 MEKEIRKEVDDAIAKAKDCPMPEPSELFTNVYV--KGFGTESFGPDRK 382

[Close]

BLAST of Cucsat.G1469 vs. TAIR 10
Match: AT1G24180.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.5e-64
Identity = 141/377 (37.40%), Postives = 211/377 (55.97%), Query Frame = 0


Query: 43  LPSLSKSNTRCRSSPVVAVSDVVKEKKL--KPSSNLLITKEEGLVLYEDMILGREFEDMC 102
           LPS  + +    SSP+   + V     L   PS ++  + EE L  + DM   R  E + 
Sbjct: 21  LPSSIRRHVSTDSSPITIETAVPFTSHLCESPSRSVETSSEEILAFFRDMARMRRME-IA 80

Query: 103 AQMYYRGKMF-GFVHLYNGQEAVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSE 162
           A   Y+ K+  GF HLY+GQEA++ G    +T++D ++++YRDH   + +G    +  SE
Sbjct: 81  ADSLYKAKLIRGFCHLYDGQEALAVGMEAAITKKDAIITSYRDHCTFIGRGGKLVDAFSE 140

Query: 163 LFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQD 222
           L G+ TGC  G+GGSMH + K+ +  GG   +G  IP+  G AF  KY ++       + 
Sbjct: 141 LMGRKTGCSHGKGGSMHFYKKDASFYGGHGIVGAQIPLGCGLAFAQKYNKD-------EA 200

Query: 223 VTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPA 282
           VT A +GDG  N GQ FE LN++ALW LP + V ENN + +G +  R+   P  +K+G  
Sbjct: 201 VTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTATWRSAKSPAYFKRGDY 260

Query: 283 FGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVECETYRFRGHSLADP-DELRDPDE 342
             +PG+ VDGMD L V++  K A   A +  GP ++E +TYR+ GHS++DP    R  DE
Sbjct: 261 --VPGLKVDGMDALAVKQACKFAKEHALK-NGPIILEMDTYRYHGHSMSDPGSTYRTRDE 320

Query: 343 -KARYAARDPIAALKKYMLENKLANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLE 402
                  RDPI  ++K +L + +A E+ELK ++ +I + V++AV  A ESP P  S+L  
Sbjct: 321 ISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIRKEVDDAVAQAKESPIPDASELFT 380

Query: 403 NVFADPKGF-GIGPDGK 414
           N++    G    G D K
Sbjct: 381 NMYVKDCGVESFGADRK 386

[Close]

BLAST of Cucsat.G1469 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 8.4e-28
Identity = 86/327 (26.30%), Postives = 160/327 (48.93%), Query Frame = 0


Query: 78  ITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTQRDTVV 137
           +++E  + +Y DM+  +  +++  +   +G++  F     G+EA++      LT +D + 
Sbjct: 125 VSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRL-SFYATAIGEEAINIASAAALTPQDVIF 184

Query: 138 STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 197
             YR+    L +G   +E  ++ FG  +   +G+   +H  S + N     A I   +P 
Sbjct: 185 PQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPN 244

Query: 198 ATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 257
           A GAA++ K  +   K+A      + +FGDG  + G F   LN+AA+ + P++F+  NN 
Sbjct: 245 AVGAAYSLKMDK---KDA----CAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNG 304

Query: 258 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 317
           WAI            +  KG A+G+  + VDG D L +      A   A R + P L+E 
Sbjct: 305 WAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEA 364

Query: 318 ETYRFRGHSLADPD-ELRDPDEKARY-AARDPIAALKKYMLENKLANEQELKAIKDKIVE 377
            TYR   HS +D     R   E   +  AR+P++  + ++  N   +++    ++ +I +
Sbjct: 365 LTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKK 424

Query: 378 VVEEAVQFADESPHPARSQLLENVFAD 403
            + EA++ A+++  P     L+N+F+D
Sbjct: 425 EMLEALRVAEKTEKPN----LQNMFSD 439

[Close]

BLAST of Cucsat.G1469 vs. TAIR 10
Match: AT5G09300.2 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 8.4e-28
Identity = 86/327 (26.30%), Postives = 160/327 (48.93%), Query Frame = 0


Query: 78  ITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTQRDTVV 137
           +++E  + +Y DM+  +  +++  +   +G++  F     G+EA++      LT +D + 
Sbjct: 54  VSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRL-SFYATAIGEEAINIASAAALTPQDVIF 113

Query: 138 STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 197
             YR+    L +G   +E  ++ FG  +   +G+   +H  S + N     A I   +P 
Sbjct: 114 PQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPN 173

Query: 198 ATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 257
           A GAA++ K  +   K+A      + +FGDG  + G F   LN+AA+ + P++F+  NN 
Sbjct: 174 AVGAAYSLKMDK---KDA----CAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNG 233

Query: 258 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 317
           WAI            +  KG A+G+  + VDG D L +      A   A R + P L+E 
Sbjct: 234 WAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEA 293

Query: 318 ETYRFRGHSLADPD-ELRDPDEKARY-AARDPIAALKKYMLENKLANEQELKAIKDKIVE 377
            TYR   HS +D     R   E   +  AR+P++  + ++  N   +++    ++ +I +
Sbjct: 294 LTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKK 353

Query: 378 VVEEAVQFADESPHPARSQLLENVFAD 403
            + EA++ A+++  P     L+N+F+D
Sbjct: 354 EMLEALRVAEKTEKPN----LQNMFSD 368

The following BLAST results are available for this feature:

BLAST of Cucsat.G1469 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24457	7.3e-194	80.38	Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Arabidopsi...	[more]
Q7XTJ3	2.3e-179	74.25	Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Oryza sati...	[more]
Q1XDM0	9.7e-130	68.18	Pyruvate dehydrogenase E1 component subunit alpha OS=Pyropia yezoensis OX=2788 G...	[more]
P51267	4.1e-128	66.67	Pyruvate dehydrogenase E1 component subunit alpha OS=Porphyra purpurea OX=2787 G...	[more]
Q92IS3	2.3e-75	39.88	Pyruvate dehydrogenase E1 component subunit alpha OS=Rickettsia conorii (strain ...	[more]

BLAST of Cucsat.G1469 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140530.1	0.0	100.00	pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis sati...	[more]
XP_008459830.1	8.26e-313	98.13	PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [C...	[more]
XP_038875269.1	2.96e-306	96.03	pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Benincasa hi...	[more]
XP_022992260.1	6.84e-296	92.99	pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita ma...	[more]
XP_022929856.1	3.95e-295	92.99	pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita mo...	[more]

BLAST of Cucsat.G1469 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KFF3	0.0	100.00	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=...	[more]
A0A5A7TEC1	4.00e-313	98.13	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX...	[more]
A0A1S3CB66	4.00e-313	98.13	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A6J1JV75	3.31e-296	92.99	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1EPB1	1.91e-295	92.99	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 ...	[more]

BLAST of Cucsat.G1469 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G01090.1	5.2e-195	80.38	pyruvate dehydrogenase E1 alpha	[more]
AT1G59900.1	1.2e-66	40.23	pyruvate dehydrogenase complex E1 alpha subunit	[more]
AT1G24180.1	2.5e-64	37.40	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G09300.1	8.4e-28	26.30	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G09300.2	8.4e-28	26.30	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017597	Pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit, subgroup y	TIGRFAM	TIGR03182	TIGR03182	coord: 81..335 e-value: 4.7E-120 score: 398.2
None	No IPR available	GENE3D	3.40.50.970		coord: 46..336 e-value: 4.8E-101 score: 340.4
None	No IPR available	PANTHER	PTHR11516:SF58	PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA	coord: 5..336
None	No IPR available	PANTHER	PTHR11516	PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR	coord: 5..336
None	No IPR available	CDD	cd02000	TPP_E1_PDC_ADC_BCADC	coord: 86..332 e-value: 2.58576E-132 score: 376.836
IPR001017	Dehydrogenase, E1 component	PFAM	PF00676	E1_dh	coord: 89..331 e-value: 1.5E-68 score: 230.9
IPR029061	Thiamin diphosphate-binding fold	SUPERFAMILY	52518	Thiamin diphosphate-binding fold (THDP-binding)	coord: 75..330

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G1469.T1	Cucsat.G1469.T1	mRNA
Cucsat.G1469.T3	Cucsat.G1469.T3	mRNA
Cucsat.G1469.T2	Cucsat.G1469.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006086	acetyl-CoA biosynthetic process from pyruvate
biological_process	GO:0006096	glycolytic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0004739	pyruvate dehydrogenase (acetyl-transferring) activity
molecular_function	GO:0016624	oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor