Cucsat.G13395.T2 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G13395.T2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	cytochrome P450 CYP82D47-like
Location	ctg1838: 5914295 .. 5915776 (+)
RNA-Seq Expression	Cucsat.G13395.T2
Synteny	Cucsat.G13395.T2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TAGCCACACTCGAACTCCTGACGAACCACCGTGTGGACCAGCTCCAATACATAAGAGCATTTGAGGTCGAGACATGGATGAAGGAACTTTATGAGTTATGGAGGCTCAATAACAAAGGTGAAAAAGTGGTGGTGGAAATGAAAAAGAGATTGGCAGATGTAACGCTGAATACAATGTTCAAGATGGTGATTGGGAAGAAATTTTCCTCTACGGAGTATGGTAATGAAAAGTTTCAAAAGGTGTTGATAGAGTTCTTTGGATTGTTTGGTATATTTATTCTATCGGATTCGTTTCCATTTCTGAGTTGGTTGGACTTGGGAGGACATAAGAAAGTCATGAAGAAGACAGCCAAAATAATAGATGAGGTGTTTGATAAATTCCTCAAAGAGCATCGGGAGAGGATAAATAATTTTGGTGAACTACCGGTGGCGGAAAAGGACTTCATGGATGTTATGATTTCTACAGTTGAAGACGATGGACAACATTTCAATTGCCATGTTGATACTGTTATTAAAGCTACATGCTTGGTAAGCAAAATATATTAGGGTATGCAAGTCTGTCTTATAGATTATAATTGCTATTTGCTAATAATGGAAGTCTATTAACAATAGTCTTAATTCTATCAACTTTATACCATTATCACCAACACAATTATATTATCGTGCTATATTTCACATTTTAGATTTAATTAGCTTTAAAATTGTTGTTATGGATTTAATTAGAAACATTTTTAAATAATGTAAAATGAATGGAAATATTTGTAAAATATAGCGAAATTTTCGATTTTCAGTTATATTAGGTAAATATTTTCAACCATTTTCGATTTAGACTTTTAAAATTAAAACTTAGGAAGTTTGTGTGATTACAGTAGCTTAGAAATTTTAACGTTAATTTTATATCTATGATTTTGTAACAAAAGGGGCTTAAAGTATGAAGAAATTCAACGTAGATTTATGAGTATTAGTTTGCAATGTTGAATGTTGAGATCTAGACTATTTGGTTTTTCAAAAAAGTATACATTTCATTTGTAAAATGACATATTTAGTTATGATATTAATAAATTTGGTATGATTCATTTTAACGCAGAATATGATATTAGGTGGATTTGACACAACAACAGTTACAATGACATGGGCTCTTTGTTTACTTCTCAACAATAAAGAAGCACTCAAGAAGGCTCAAGTTGAATTAGATGAACAAGTTGGGAGAGAAAGACAAGTGAAAGAGACGGATTTGAAAAATCTACCCTATCTCCAAGCTATTGTTAAGGAAACTCTACGATTGTATCCTGCTGCACCACTGTTAGTCCCTCATGAATCTATAGAAGATTGCACAGTTGCTGGCTATCACATACCGAAAGGAACACGCCTCATAGTTAATGTTCAAAAACTTCAAAGAGATCCACTTGTTTGGGAGGATCCATTTGAATTTCGACCAGAAAGATTTTTAACAAGTCAAAAGAATTTTGATGTTAGAGGACAA

Coding sequence (CDS)

GCCACACTCGAACTCCTGACGAACCACCGTGTGGACCAGCTCCAATACATAAGAGCATTTGAGGTCGAGACATGGATGAAGGAACTTTATGAGTTATGGAGGCTCAATAACAAAGGTGAAAAAGTGGTGGTGGAAATGAAAAAGAGATTGGCAGATGTAACGCTGAATACAATGTTCAAGATGGTGATTGGGAAGAAATTTTCCTCTACGGAGTATGGTAATGAAAAGTTTCAAAAGGTGTTGATAGAGTTCTTTGGATTGTTTGGTATATTTATTCTATCGGATTCGTTTCCATTTCTGAGTTGGTTGGACTTGGGAGGACATAAGAAAGTCATGAAGAAGACAGCCAAAATAATAGATGAGGTGTTTGATAAATTCCTCAAAGAGCATCGGGAGAGGATAAATAATTTTGGTGAACTACCGGTGGCGGAAAAGGACTTCATGGATGTTATGATTTCTACAGTTGAAGACGATGGACAACATTTCAATTGCCATGTTGATACTGTTATTAAAGCTACATGCTTGGTAAGCAAAATATATTAG

Protein sequence

ATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVVVEMKKRLADVTLNTMFKMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCLVSKIY

Homology

[Close]

BLAST of Cucsat.G13395.T2 vs. ExPASy Swiss-Prot
Match: H2DH24 (Cytochrome P450 CYP82D47 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 8.7e-32
Identity = 78/182 (42.86%), Postives = 122/182 (67.03%), Query Frame = 0


Query: 2   TLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGE-KVVVEMKKRLADVTLNTMFK 61
           +L+LL++ R++ L+++R  E+E  MK+LY++W     G  +V+VEMKK   D+TLN + +
Sbjct: 19  SLQLLSSRRLELLKHVRISEMEISMKQLYKIWSKKKDGSGRVLVEMKKLFGDLTLNVVLR 78

Query: 62  MVIGKKFSSTEYGNE-----KFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKT 121
           MV GK++      N+     + ++V  EFF L G+F+++DS PFL WLDLGG++K MKKT
Sbjct: 79  MVAGKRYFGGGNANDDEEARRCRRVWREFFRLHGVFVVADSLPFLRWLDLGGYEKAMKKT 138

Query: 122 AKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVED-DGQHFNCHVDTVIKATC 177
           AK +D +   +L+EHR + N+  E    ++DFMDVM+S V++ D   F+   D VIK+TC
Sbjct: 139 AKEMDNIVSGWLEEHRMKRNSSDE-DNTQQDFMDVMLSAVKNVDLCGFD--ADVVIKSTC 197

[Close]

BLAST of Cucsat.G13395.T2 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.2e-28
Identity = 74/181 (40.88%), Postives = 112/181 (61.88%), Query Frame = 0


Query: 2   TLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEK--VVVEMKKRLADVTLNTMF 61
           T E L+N R++Q  +IR  EV T +KEL+++W   NK E    +V++K+ LA +T N + 
Sbjct: 140 TFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVDIKQWLAYLTFNMVV 199

Query: 62  KMVIGKKFSSTEY--GNEK---FQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKK 121
           +MV+GK++    +  G +K   F K + EF  L G F ++D  P L WLDLGGH+K MK 
Sbjct: 200 RMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPCLRWLDLGGHEKAMKA 259

Query: 122 TAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATC 176
            AK +D++  ++L+EHR++    GE   +++DFMDVMIS + +  Q      DT+ KAT 
Sbjct: 260 NAKEVDKLLSEWLEEHRQK-KLLGENVESDRDFMDVMISAL-NGAQIGAFDADTICKATS 318

[Close]

BLAST of Cucsat.G13395.T2 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.0e-27
Identity = 71/184 (38.59%), Postives = 111/184 (60.33%), Query Frame = 0


Query: 1   ATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVVVEMKKRLADVTLNTMFK 60
           AT+ELL+N R+  L+++R  E+   +K+LY LW  N   + V+V++K  L D+TLN + +
Sbjct: 134 ATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIVR 193

Query: 61  MVIGKKF------SSTEYGNEKFQ--KVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVM 120
           MV GK++       S+E   E  Q  K + +FF L GIF +SD+FP LS+ DL GH+K M
Sbjct: 194 MVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEM 253

Query: 121 KKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDG-QHFNCHVDTVIK 176
           K+T   +D + +++++ HR++   F      + DF+DVM+S  E     H     +T IK
Sbjct: 254 KQTGSELDVILERWIENHRQQ-RKFSGTKENDSDFIDVMMSLAEQGKLSHLQYDANTSIK 313

[Close]

BLAST of Cucsat.G13395.T2 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.9e-26
Identity = 70/177 (39.55%), Postives = 114/177 (64.41%), Query Frame = 0


Query: 4   ELLTNHRVDQLQYIRAFEVETWMKELYELWR-LNNKGEKVVVEMKKRLADVTLNTMFKMV 63
           E+L++ RV+QLQ +R  EV+  + ELY++WR   N+ +   VE+K+  A    N + +MV
Sbjct: 144 EILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVELKQWFAQPIFNMVLRMV 203

Query: 64  IGKKF---SSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKII 123
           +GK+F   ++T+   EK  K + EF  L G+F + D+ P+L WLD GG++K MK+TAK +
Sbjct: 204 VGKRFLSATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRWLDFGGYEKAMKETAKEL 263

Query: 124 DEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFN-CHVDTVIKATCL 176
           D +  ++L+EHR++    GE     +DFM+VM+S++  DG+  +    DT+IK+T L
Sbjct: 264 DVMISEWLEEHRQK-RALGEGVDGAQDFMNVMLSSL--DGKTIDGIDADTLIKSTVL 317

[Close]

BLAST of Cucsat.G13395.T2 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.5e-26
Identity = 75/181 (41.44%), Postives = 107/181 (59.12%), Query Frame = 0


Query: 3   LELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVVVEMKKRLADVTLNTMFKMV 62
           LE L+N R+  L+++   E+    KELY+LW   N  +  +V+MK+ LAD+TLN   KMV
Sbjct: 139 LEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNI-DFCLVDMKQWLADLTLNMSVKMV 198

Query: 63  IGKKF-------SSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKT 122
           +GK+F       +  E  +    K L   F L G F+LSD  P+L WLDLGGH+K MK+T
Sbjct: 199 VGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYLRWLDLGGHEKEMKRT 258

Query: 123 AKIIDEVFDKFLKEH-RERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATC 176
            K +D +F  +L EH R+R++  G     ++DFMDVM+S +E+        VDT+ K  C
Sbjct: 259 VKELDILFKGWLDEHKRKRLS--GGKEDDDQDFMDVMLSILEE--SKLGNDVDTINKTAC 314

[Close]

BLAST of Cucsat.G13395.T2 vs. NCBI nr
Match: XP_011652397.1 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN60056.2 hypothetical protein Csa_002536 [Cucumis sativus])

HSP 1 Score: 350 bits (897), Expect = 2.00e-116
Identity = 171/175 (97.71%), Postives = 173/175 (98.86%), Query Frame = 0


Query: 1   ATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVVVEMKKRLADVTLNTMFK 60
           ATLELLTNHRVDQLQYIRAFEVETWM+ELYELWRLNNKGEKVVVEMKKRLADVTLNTMFK
Sbjct: 137 ATLELLTNHRVDQLQYIRAFEVETWMEELYELWRLNNKGEKVVVEMKKRLADVTLNTMFK 196

Query: 61  MVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKIID 120
           MVIGKKFSS EYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKI+D
Sbjct: 197 MVIGKKFSSMEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKIMD 256

Query: 121 EVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCL 175
           EVFDKFLKEHRERINNFGELP AEKDFMDVMISTVEDDGQHFNCHVDTVIKATCL
Sbjct: 257 EVFDKFLKEHRERINNFGELPAAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCL 311

[Close]

BLAST of Cucsat.G13395.T2 vs. NCBI nr
Match: KAA0032758.1 (cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa] >TYJ98789.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa])

HSP 1 Score: 331 bits (849), Expect = 7.69e-112
Identity = 160/179 (89.39%), Postives = 170/179 (94.97%), Query Frame = 0


Query: 1   ATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVVVEMKKRLADVTLNTMFK 60
           ATLELLTNHRV QLQYIRAFE++TWMKELYELWRLNNKGEKVVVEMKKRLAD+TLNT+FK
Sbjct: 137 ATLELLTNHRVAQLQYIRAFEIKTWMKELYELWRLNNKGEKVVVEMKKRLADITLNTIFK 196

Query: 61  MVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKIID 120
           MVIGK+FSS EYGNEKFQ VLIEFFGLFGIFI SDSFPFLSWLDLGGH+K MKKTAKIID
Sbjct: 197 MVIGKRFSSIEYGNEKFQNVLIEFFGLFGIFIPSDSFPFLSWLDLGGHEKAMKKTAKIID 256

Query: 121 EVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCLVSKI 179
           EVFDKFLKE RERINNFGE+  AEK FMD+MISTV+DDGQHFNCHVDTVIKATCLVSK+
Sbjct: 257 EVFDKFLKERRERINNFGEVEAAEKGFMDIMISTVQDDGQHFNCHVDTVIKATCLVSKL 315

[Close]

BLAST of Cucsat.G13395.T2 vs. NCBI nr
Match: XP_008464769.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo])

HSP 1 Score: 325 bits (833), Expect = 9.25e-107
Identity = 157/175 (89.71%), Postives = 166/175 (94.86%), Query Frame = 0


Query: 1   ATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVVVEMKKRLADVTLNTMFK 60
           ATLELLTNHRV QLQYIRAFE++TWMKELYELWRLNNKGEKVVVEMKKRLAD+TLNT+FK
Sbjct: 137 ATLELLTNHRVAQLQYIRAFEIKTWMKELYELWRLNNKGEKVVVEMKKRLADITLNTIFK 196

Query: 61  MVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKIID 120
           MVIGK+FSS EYGNEKFQ VLIEFFGLFGIFI SDSFPFLSWLDLGGH+K MKKTAKIID
Sbjct: 197 MVIGKRFSSIEYGNEKFQNVLIEFFGLFGIFIPSDSFPFLSWLDLGGHEKAMKKTAKIID 256

Query: 121 EVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCL 175
           EVFDKFLKE RERINNFGE+  AEK FMD+MISTV+DDGQHFNCHVDTVIKATCL
Sbjct: 257 EVFDKFLKERRERINNFGEVEAAEKGFMDIMISTVQDDGQHFNCHVDTVIKATCL 311

[Close]

BLAST of Cucsat.G13395.T2 vs. NCBI nr
Match: XP_022935746.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 253 bits (645), Expect = 1.69e-78
Identity = 125/176 (71.02%), Postives = 150/176 (85.23%), Query Frame = 0


Query: 1   ATLELLTNHRVDQLQYIRAFEVETWMKELYELW-RLNNKGEKVVVEMKKRLADVTLNTMF 60
           ATLELLTNHR++QLQ+IRAFEV+ WMKEL+++  + N +GEKV VEMKK LAD+TLNT+F
Sbjct: 137 ATLELLTNHRLEQLQHIRAFEVQRWMKELHKMSIKSNERGEKVAVEMKKWLADITLNTIF 196

Query: 61  KMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKII 120
           KMVIGK+FS+ ++G E F K LI+FFGLF IF+ SDSFPFLSWLDLGGH+K MKKTAKII
Sbjct: 197 KMVIGKRFSAIQHGTENFPKALIDFFGLFHIFVPSDSFPFLSWLDLGGHEKTMKKTAKII 256

Query: 121 DEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCL 175
           DE F KFL++HRER N+ GE   AEKDFMDVMI+ VE+DGQHF+   DT+IKATCL
Sbjct: 257 DEAFHKFLQQHRERRND-GEAETAEKDFMDVMIARVEEDGQHFSYDADTIIKATCL 311

[Close]

BLAST of Cucsat.G13395.T2 vs. NCBI nr
Match: XP_023535451.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 251 bits (640), Expect = 9.47e-78
Identity = 124/176 (70.45%), Postives = 149/176 (84.66%), Query Frame = 0


Query: 1   ATLELLTNHRVDQLQYIRAFEVETWMKELYELW-RLNNKGEKVVVEMKKRLADVTLNTMF 60
           ATLELLTNHR++QLQ+IRAFEV+ WM EL+++  + N +GEKV VEMKK LAD+TLNT+F
Sbjct: 137 ATLELLTNHRLEQLQHIRAFEVQRWMNELHKMSIKSNERGEKVAVEMKKWLADITLNTIF 196

Query: 61  KMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKII 120
           KMVIGK+FS+ ++GNE F K LI+FFGLF IF+ SDSFPFLSWLDLGGH+K MKKTAK+I
Sbjct: 197 KMVIGKRFSAIQHGNENFPKALIDFFGLFHIFVPSDSFPFLSWLDLGGHEKTMKKTAKVI 256

Query: 121 DEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCL 175
           DE F KFL++HRER N+  E   AEKDFMDVMI+ VE+DGQHF+   DTVIKATCL
Sbjct: 257 DEAFHKFLQQHRERRND-AEAETAEKDFMDVMIARVEEDGQHFSYDADTVIKATCL 311

[Close]

BLAST of Cucsat.G13395.T2 vs. ExPASy TrEMBL
Match: A0A0A0LIS1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G853140 PE=3 SV=1)

HSP 1 Score: 350 bits (897), Expect = 1.43e-119
Identity = 171/175 (97.71%), Postives = 173/175 (98.86%), Query Frame = 0


Query: 1   ATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVVVEMKKRLADVTLNTMFK 60
           ATLELLTNHRVDQLQYIRAFEVETWM+ELYELWRLNNKGEKVVVEMKKRLADVTLNTMFK
Sbjct: 137 ATLELLTNHRVDQLQYIRAFEVETWMEELYELWRLNNKGEKVVVEMKKRLADVTLNTMFK 196

Query: 61  MVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKIID 120
           MVIGKKFSS EYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKI+D
Sbjct: 197 MVIGKKFSSMEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKIMD 256

Query: 121 EVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCL 175
           EVFDKFLKEHRERINNFGELP AEKDFMDVMISTVEDDGQHFNCHVDTVIKATCL
Sbjct: 257 EVFDKFLKEHRERINNFGELPAAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCL 311

[Close]

BLAST of Cucsat.G13395.T2 vs. ExPASy TrEMBL
Match: A0A5A7SQD8 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00980 PE=3 SV=1)

HSP 1 Score: 331 bits (849), Expect = 3.72e-112
Identity = 160/179 (89.39%), Postives = 170/179 (94.97%), Query Frame = 0


Query: 1   ATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVVVEMKKRLADVTLNTMFK 60
           ATLELLTNHRV QLQYIRAFE++TWMKELYELWRLNNKGEKVVVEMKKRLAD+TLNT+FK
Sbjct: 137 ATLELLTNHRVAQLQYIRAFEIKTWMKELYELWRLNNKGEKVVVEMKKRLADITLNTIFK 196

Query: 61  MVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKIID 120
           MVIGK+FSS EYGNEKFQ VLIEFFGLFGIFI SDSFPFLSWLDLGGH+K MKKTAKIID
Sbjct: 197 MVIGKRFSSIEYGNEKFQNVLIEFFGLFGIFIPSDSFPFLSWLDLGGHEKAMKKTAKIID 256

Query: 121 EVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCLVSKI 179
           EVFDKFLKE RERINNFGE+  AEK FMD+MISTV+DDGQHFNCHVDTVIKATCLVSK+
Sbjct: 257 EVFDKFLKERRERINNFGEVEAAEKGFMDIMISTVQDDGQHFNCHVDTVIKATCLVSKL 315

[Close]

BLAST of Cucsat.G13395.T2 vs. ExPASy TrEMBL
Match: A0A1S3CMD6 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502577 PE=3 SV=1)

HSP 1 Score: 325 bits (833), Expect = 4.48e-107
Identity = 157/175 (89.71%), Postives = 166/175 (94.86%), Query Frame = 0


Query: 1   ATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVVVEMKKRLADVTLNTMFK 60
           ATLELLTNHRV QLQYIRAFE++TWMKELYELWRLNNKGEKVVVEMKKRLAD+TLNT+FK
Sbjct: 137 ATLELLTNHRVAQLQYIRAFEIKTWMKELYELWRLNNKGEKVVVEMKKRLADITLNTIFK 196

Query: 61  MVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKIID 120
           MVIGK+FSS EYGNEKFQ VLIEFFGLFGIFI SDSFPFLSWLDLGGH+K MKKTAKIID
Sbjct: 197 MVIGKRFSSIEYGNEKFQNVLIEFFGLFGIFIPSDSFPFLSWLDLGGHEKAMKKTAKIID 256

Query: 121 EVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCL 175
           EVFDKFLKE RERINNFGE+  AEK FMD+MISTV+DDGQHFNCHVDTVIKATCL
Sbjct: 257 EVFDKFLKERRERINNFGEVEAAEKGFMDIMISTVQDDGQHFNCHVDTVIKATCL 311

[Close]

BLAST of Cucsat.G13395.T2 vs. ExPASy TrEMBL
Match: A0A6J1F5K6 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442570 PE=3 SV=1)

HSP 1 Score: 253 bits (645), Expect = 8.19e-79
Identity = 125/176 (71.02%), Postives = 150/176 (85.23%), Query Frame = 0


Query: 1   ATLELLTNHRVDQLQYIRAFEVETWMKELYELW-RLNNKGEKVVVEMKKRLADVTLNTMF 60
           ATLELLTNHR++QLQ+IRAFEV+ WMKEL+++  + N +GEKV VEMKK LAD+TLNT+F
Sbjct: 137 ATLELLTNHRLEQLQHIRAFEVQRWMKELHKMSIKSNERGEKVAVEMKKWLADITLNTIF 196

Query: 61  KMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKII 120
           KMVIGK+FS+ ++G E F K LI+FFGLF IF+ SDSFPFLSWLDLGGH+K MKKTAKII
Sbjct: 197 KMVIGKRFSAIQHGTENFPKALIDFFGLFHIFVPSDSFPFLSWLDLGGHEKTMKKTAKII 256

Query: 121 DEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCL 175
           DE F KFL++HRER N+ GE   AEKDFMDVMI+ VE+DGQHF+   DT+IKATCL
Sbjct: 257 DEAFHKFLQQHRERRND-GEAETAEKDFMDVMIARVEEDGQHFSYDADTIIKATCL 311

[Close]

BLAST of Cucsat.G13395.T2 vs. ExPASy TrEMBL
Match: A0A6J1IFL6 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476811 PE=3 SV=1)

HSP 1 Score: 246 bits (628), Expect = 3.39e-76
Identity = 122/176 (69.32%), Postives = 147/176 (83.52%), Query Frame = 0


Query: 1   ATLELLTNHRVDQLQYIRAFEVETWMKELYELW-RLNNKGEKVVVEMKKRLADVTLNTMF 60
           ATLELLTNHR++QLQ+IR FEV+ WMKEL+++  R N +G+KV VEMKK LAD+TLNT+F
Sbjct: 137 ATLELLTNHRLEQLQHIRVFEVQRWMKELHKMSIRSNERGDKVAVEMKKWLADITLNTIF 196

Query: 61  KMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKII 120
           KMVIGK+FS+ ++G E F K LI+FFGLF IF+ SDSFPFLSWLDLGGH+K MKKTAK+I
Sbjct: 197 KMVIGKRFSAIQHGTENFPKALIDFFGLFHIFVPSDSFPFLSWLDLGGHEKTMKKTAKVI 256

Query: 121 DEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCL 175
           DE F KFL++HRER N  G+   AE+DFMDVMIS VE+D QHF+   DTVIKATCL
Sbjct: 257 DEAFHKFLQQHRERRNE-GQAETAEQDFMDVMISRVEEDEQHFSYDADTVIKATCL 311

The following BLAST results are available for this feature:

BLAST of Cucsat.G13395.T2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
H2DH24	8.7e-32	42.86	Cytochrome P450 CYP82D47 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
O49858	1.2e-28	40.88	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]
Q9SZ46	1.0e-27	38.59	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]
O49859	1.9e-26	39.55	Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1	[more]
A0A0N7F297	2.5e-26	41.44	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]

BLAST of Cucsat.G13395.T2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652397.1	2.00e-116	97.71	cytochrome P450 CYP82D47 [Cucumis sativus] >KGN60056.2 hypothetical protein Csa_...	[more]
KAA0032758.1	7.69e-112	89.39	cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa] >TYJ98789.1 cytochrome ...	[more]
XP_008464769.1	9.25e-107	89.71	PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo]	[more]
XP_022935746.1	1.69e-78	71.02	cytochrome P450 CYP82D47-like [Cucurbita moschata]	[more]
XP_023535451.1	9.47e-78	70.45	cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G13395.T2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIS1	1.43e-119	97.71	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G853140 PE=3 SV=1	[more]
A0A5A7SQD8	3.72e-112	89.39	Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3CMD6	4.48e-107	89.71	cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502577 PE=3 SV=1	[more]
A0A6J1F5K6	8.19e-79	71.02	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442570 PE=3...	[more]
A0A6J1IFL6	3.39e-76	69.32	cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476811 PE=3 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 22..163 e-value: 7.0E-11 score: 41.5
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..176 e-value: 2.3E-17 score: 64.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 4..163
None	No IPR available	PANTHER	PTHR24281	STEROID 21-HYDROXYLASE-RELATED	coord: 1..174
None	No IPR available	PANTHER	PTHR24281:SF48	CYTOCHROME P450	coord: 1..174

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G13395	Cucsat.G13395	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13395.T2.E1	Cucsat.G13395.T2.E1	exon
Cucsat.G13395.T2.E2	Cucsat.G13395.T2.E2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13395.T2.C1	Cucsat.G13395.T2.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13395.T2	Cucsat.G13395.T2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen