Cucsat.G13395 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G13395
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	cytochrome P450 CYP82D47-like
Location	ctg1838: 5913942 .. 5916111 (+)
RNA-Seq Expression	Cucsat.G13395
Synteny	Cucsat.G13395

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCGCAATCCTACCCATCCACATCTAATATATATATTATTTTCGTTCCACCATAGAAATAATAAATTTTTAAATCCATCACGGGTAGACCTTATGATATCTTCCCAATGAAATTATCCACTCACATAACATTAGCCAAAATGGCGGATGCATATGGACCAATGTTCACATTTAGGTTCGGTATGAAAAGGGCGTTGATTGTAAGCAATTGGGATATAGCAAAAGAGATCTTTACGACAAACGACAGAATCTTCGCGTCTCGTCCAAAGCTTGTAGCCTAAAAAATCCTTGCCTATGACTATGCCATGATGGGATTCAGCCCGTACAGTCCACACTGGCGCTATGTACGAAAAATAGCCACACTCGAACTCCTGACGAACCACCGTGTGGACCAGCTCCAATACATAAGAGCATTTGAGGTCGAGACATGGATGAAGGAACTTTATGAGTTATGGAGGCTCAATAACAAAGGTGAAAAAGTGGTGGTGGAAATGAAAAAGAGATTGGCAGATGTAACGCTGAATACAATGTTCAAGATGGTGATTGGGAAGAAATTTTCCTCTACGGAGTATGGTAATGAAAAGTTTCAAAAGGTGTTGATAGAGTTCTTTGGATTGTTTGGTATATTTATTCTATCGGATTCGTTTCCATTTCTGAGTTGGTTGGACTTGGGAGGACATAAGAAAGTCATGAAGAAGACAGCCAAAATAATAGATGAGGTGTTTGATAAATTCCTCAAAGAGCATCGGGAGAGGATAAATAATTTTGGTGAACTACCGGTGGCGGAAAAGGACTTCATGGATGTTATGATTTCTACAGTTGAAGACGATGGACAACATTTCAATTGCCATGTTGATACTGTTATTAAAGCTACATGCTTGGTAAGCAAAATATATTAGGGTATGCAAGTCTGTCTTATAGATTATAATTGCTATTTGCTAATAATGGAAGTCTATTAACAATAGTCTTAATTCTATCAACTTTATACCATTATCACCAACACAATTATATTATCGTGCTATATTTCACATTTTAGATTTAATTAGCTTTAAAATTGTTGTTATGGATTTAATTAGAAACATTTTTAAATAATGTAAAATGAATGGAAATATTTGTAAAATATAGCGAAATTTTCGATTTTCAGTTATATTAGGTAAATATTTTCAACCATTTTCGATTTAGACTTTTAAAATTAAAACTTAGGAAGTTTGTGTGATTACAGTAGCTTAGAAATTTTAACGTTAATTTTATATCTATGATTTTGTAACAAAAGGGGCTTAAAGTATGAAGAAATTCAACGTAGATTTATGAGTATTAGTTTGCAATGTTGAATGTTGAGATCTAGACTATTTGGTTTTTCAAAAAAGTATACATTTCATTTGTAAAATGACATATTTAGTTATGATATTAATAAATTTGGTATGATTCATTTTAACGCAGAATATGATATTAGGTGGATTTGACACAACAACAGTTACAATGACATGGGCTCTTTGTTTACTTCTCAACAATAAAGAAGCACTCAAGAAGGCTCAAGTTGAATTAGATGAACAAGTTGGGAGAGAAAGACAAGTGAAAGAGACGGATTTGAAAAATCTACCCTATCTCCAAGCTATTGTTAAGGAAACTCTACGATTGTATCCTGCTGCACCACTGTTAGTCCCTCATGAATCTATAGAAGATTGCACAGTTGCTGGCTATCACATACCGAAAGGAACACGCCTCATAGTTAATGTTCAAAAACTTCAAAGAGATCCACTTGTTTGGGAGGATCCATTTGAATTTCGACCAGAAAGATTTTTAACAAGTCAAAAGAATTTTGATGTTAGAGGACAAAATCCCCAATTCATACCGTTTGGAAATGGTCGAAGGATGTGCCCTGCAATCTCATTTGCACTTCAAATAATCTATCTTACACTCTCAAACTTTCTTCATGGGTTTGAAATTGATAGACCATCAGAAGAACTACTTGACATGGAAGAGAGTATTGGATTGACAAGCCTTAAAAAAACCCCACTCGAAGTTGTTTTAACTCCACGCCTACCTTCACATCTTTATGGTTAATATTTTTTCTTGTCCTTTTTTTTAAGTTTAATTTTATGTAATAAAAAGAAAATGGTGGGGTGGGGTAATAATTCACCCTTATTTTAAATTATATCAAGTTTGGTTTT

Coding sequence (CDS)

ATGATGGGATTCAGCCCGTACAGTCCACACTGGCGCTATGTACGAAAAATAGCCACACTCGAACTCCTGACGAACCACCGTGTGGACCAGCTCCAATACATAAGAGCATTTGAGGTCGAGACATGGATGAAGGAACTTTATGAGTTATGGAGGCTCAATAACAAAGGTGAAAAAGTGGTGGTGGAAATGAAAAAGAGATTGGCAGATGTAACGCTGAATACAATGTTCAAGATGGTGATTGGGAAGAAATTTTCCTCTACGGAGTATGGTAATGAAAAGTTTCAAAAGGTGTTGATAGAGTTCTTTGGATTGTTTGGTATATTTATTCTATCGGATTCGTTTCCATTTCTGAGTTGGTTGGACTTGGGAGGACATAAGAAAGTCATGAAGAAGACAGCCAAAATAATAGATGAGGTGTTTGATAAATTCCTCAAAGAGCATCGGGAGAGGATAAATAATTTTGGTGAACTACCGGTGGCGGAAAAGGACTTCATGGATGTTATGATTTCTACAGTTGAAGACGATGGACAACATTTCAATTGCCATGTTGATACTGTTATTAAAGCTACATGCTTGAATATGATATTAGGTGGATTTGACACAACAACAGTTACAATGACATGGGCTCTTTGTTTACTTCTCAACAATAAAGAAGCACTCAAGAAGGCTCAAGTTGAATTAGATGAACAAGTTGGGAGAGAAAGACAAGTGAAAGAGACGGATTTGAAAAATCTACCCTATCTCCAAGCTATTGTTAAGGAAACTCTACGATTGTATCCTGCTGCACCACTGTTAGTCCCTCATGAATCTATAGAAGATTGCACAGTTGCTGGCTATCACATACCGAAAGGAACACGCCTCATAGTTAATGTTCAAAAACTTCAAAGAGATCCACTTGTTTGGGAGGATCCATTTGAATTTCGACCAGAAAGATTTTTAACAAGTCAAAAGAATTTTGATGTTAGAGGACAAAATCCCCAATTCATACCGTTTGGAAATGGTCGAAGGATGTGCCCTGCAATCTCATTTGCACTTCAAATAATCTATCTTACACTCTCAAACTTTCTTCATGGGTTTGAAATTGATAGACCATCAGAAGAACTACTTGACATGGAAGAGAGTATTGGATTGACAAGCCTTAAAAAAACCCCACTCGAAGTTGTTTTAACTCCACGCCTACCTTCACATCTTTATGGTTAA

Protein sequence

MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVVVEMKKRLADVTLNTMFKMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWLDLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFNCHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVKETDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDPLVWEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGFEIDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLYG

Homology

[Close]

BLAST of Cucsat.G13395 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 1.0e-109
Identity = 204/408 (50.00%), Postives = 286/408 (70.10%), Query Frame = 0


Query: 1   MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVV 60
           + GF+PYS  WR +RKIAT+ELL+N R+  L+++R  E+   +K+LY LW  N   + V+
Sbjct: 117 VFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVM 176

Query: 61  VEMKKRLADVTLNTMFKMVIGKKF------SSTEYGNEKFQ--KVLIEFFGLFGIFILSD 120
           V++K  L D+TLN + +MV GK++       S+E   E  Q  K + +FF L GIF +SD
Sbjct: 177 VDLKSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSD 236

Query: 121 SFPFLSWLDLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTV 180
           +FP LS+ DL GH+K MK+T   +D + +++++ HR++   F      + DF+DVM+S  
Sbjct: 237 AFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQ-RKFSGTKENDSDFIDVMMSLA 296

Query: 181 EDDG-QHFNCHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQV 240
           E     H     +T IK+TCL +ILGG DT+  T+TWA+ LLLNNKE LKKAQ E+D  V
Sbjct: 297 EQGKLSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHV 356

Query: 241 GRERQVKETDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNV 300
           GR+R V+++D++NL YLQAI+KETLRLYPA PLL P E++EDCTVAGY++P GTRLIVNV
Sbjct: 357 GRDRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNV 416

Query: 301 QKLQRDPLVWEDPFEFRPERFLTSQ-KNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYL 360
            K+QRDP V+ +P EFRPERF+T + K FDVRGQN + +PFG+GRR CP  S A+Q+++L
Sbjct: 417 WKIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHL 476

Query: 361 TLSNFLHGFEIDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLY 399
            L+ FLH F++    +  +DM E+ GLT  K TPLEV+++PR+   L+
Sbjct: 477 GLARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEELF 523

[Close]

BLAST of Cucsat.G13395 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.6e-105
Identity = 204/401 (50.87%), Postives = 272/401 (67.83%), Query Frame = 0


Query: 1   MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVV 60
           M+G +PY P+WR +RKI  LE L+N R+  L+++   E+    KELY+LW   N  +  +
Sbjct: 120 MLGVAPYGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNI-DFCL 179

Query: 61  VEMKKRLADVTLNTMFKMVIGKKF-------SSTEYGNEKFQKVLIEFFGLFGIFILSDS 120
           V+MK+ LAD+TLN   KMV+GK+F       +  E  +    K L   F L G F+LSD 
Sbjct: 180 VDMKQWLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDY 239

Query: 121 FPFLSWLDLGGHKKVMKKTAKIIDEVFDKFLKEH-RERINNFGELPVAEKDFMDVMISTV 180
            P+L WLDLGGH+K MK+T K +D +F  +L EH R+R++  G     ++DFMDVM+S +
Sbjct: 240 LPYLRWLDLGGHEKEMKRTVKELDILFKGWLDEHKRKRLS--GGKEDDDQDFMDVMLSIL 299

Query: 181 EDDGQHFNCHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVG 240
           E+        VDT+ K  CL +ILGG DTT  T+TWAL LLLNN  ALKKAQ ELD  VG
Sbjct: 300 EE--SKLGNDVDTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVG 359

Query: 241 RERQVKETDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQ 300
           R+R V E+DL  L Y+ AI+KETLRLYP  PLL P    EDCT+AGYH+  GTRLIVN  
Sbjct: 360 RDRNVDESDLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAW 419

Query: 301 KLQRDPLVWEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTL 360
           K+QRDPLVW  P E++PERFL  +++ D++GQ+ + IPFG+GRR CPAIS ALQ++ LTL
Sbjct: 420 KIQRDPLVWSQPHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTL 479

Query: 361 SNFLHGFEIDRPSEELLDMEESIGLTSLKKTPLEVVLTPRL 394
           ++ LHGFE+  P++  +DM E+ G+   K TPLEV++ PR+
Sbjct: 480 AHILHGFELRTPNQNKVDMTETPGIVHAKATPLEVLVAPRI 513

[Close]

BLAST of Cucsat.G13395 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 3.4e-105
Identity = 194/404 (48.02%), Postives = 272/404 (67.33%), Query Frame = 0


Query: 2   MGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEK--V 61
           +G +PY P+WR +RKI T E L+N R++Q  +IR  EV T +KEL+++W   NK E    
Sbjct: 123 VGLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYT 182

Query: 62  VVEMKKRLADVTLNTMFKMVIGKKFSSTEY--GNEK---FQKVLIEFFGLFGIFILSDSF 121
           +V++K+ LA +T N + +MV+GK++    +  G +K   F K + EF  L G F ++D  
Sbjct: 183 LVDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGV 242

Query: 122 PFLSWLDLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVED 181
           P L WLDLGGH+K MK  AK +D++  ++L+EHR++    GE   +++DFMDVMIS + +
Sbjct: 243 PCLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQK-KLLGENVESDRDFMDVMISAL-N 302

Query: 182 DGQHFNCHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRE 241
             Q      DT+ KAT L +ILGG D+T VT+TWAL LLL N  AL KA+ E+D Q+G++
Sbjct: 303 GAQIGAFDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKD 362

Query: 242 RQVKETDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKL 301
             ++E+D+  L YLQAIVKETLRLYP AP   P E  E+C + GYHI KGTRLI N+ K+
Sbjct: 363 EYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKI 422

Query: 302 QRDPLVWEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSN 361
            RDP VW DP EF+PERFLT+ K+ D+RG N + +PFG+GRR+C  +S  L +++ TL+N
Sbjct: 423 HRDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLAN 482

Query: 362 FLHGFEIDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLY 399
            LH F+I  PS E +DM E  G T+ K TPLE+++ PR   + Y
Sbjct: 483 LLHSFDILNPSAEPVDMTEFFGFTNTKATPLEILVKPRQSPNYY 524

[Close]

BLAST of Cucsat.G13395 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 382.1 bits (980), Expect = 7.7e-105
Identity = 199/407 (48.89%), Postives = 279/407 (68.55%), Query Frame = 0


Query: 1   MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVV 60
           + GF+PYS  WR +RKIATLELL+N R+  L+++R  E+   M++LY LW      E V+
Sbjct: 117 VFGFAPYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVM 176

Query: 61  VEMKKRLADVTLNTMFKMVIGKKF-----SSTEYGNEKFQ--KVLIEFFGLFGIFILSDS 120
           V++K  L D++LN M +MV GK++      S E   E  Q  K +  FF L GIF +SD+
Sbjct: 177 VDLKSWLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDA 236

Query: 121 FPFLSWLDLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVE 180
           FP L W D  GH+K MK+T + +D + +++++ HR++    G     + DF+DVM+S  E
Sbjct: 237 FPKLGWFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSG-TKHNDSDFVDVMLSLAE 296

Query: 181 DDG-QHFNCHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVG 240
                H      T IK+TCL +ILGG +T+  T+TWA+ LLLNNK+ LKKAQ E+D  VG
Sbjct: 297 QGKFSHLQHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVG 356

Query: 241 RERQVKETDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQ 300
           R+R V+++D++NL Y+QAI+KETLRLYPA PLL   E+IEDCTVAGY++ +GTR++VNV 
Sbjct: 357 RDRNVEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVW 416

Query: 301 KLQRDPLVWEDPFEFRPERFLTSQ-KNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLT 360
           K+QRDP V+ +P EFRPERF+T + K FDVRGQN + +PFG+GRR CP  S A+Q+++L 
Sbjct: 417 KIQRDPRVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLG 476

Query: 361 LSNFLHGFEIDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLY 399
           L+ FL  F++    +  +DM ES GLT  K TPLE++++PRL   LY
Sbjct: 477 LARFLQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

[Close]

BLAST of Cucsat.G13395 vs. ExPASy Swiss-Prot
Match: O49396 (Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3)

HSP 1 Score: 375.6 bits (963), Expect = 7.2e-103
Identity = 196/398 (49.25%), Postives = 274/398 (68.84%), Query Frame = 0


Query: 11  WRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVVVEMKKRLADV 70
           W  +RKIA +ELL+N R+  L  +R  E+   +K+LY LW      E V+V++K  L D+
Sbjct: 115 WLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEPVMVDLKSWLEDM 174

Query: 71  TLNTMFKMVIGKKF------SSTEYGNE--KFQKVLIEFFGLFGIFILSDSFPFLSWLDL 130
             N + +MV GK++       S+E+  E  +++K + +FF L GIF +SD+FP L WLDL
Sbjct: 175 IANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDL 234

Query: 131 GGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDG-QHFNC 190
            GH+K MK+T + +D + +++++ HR++    G     + DF+DVM+S  E     H   
Sbjct: 235 QGHEKEMKQTRRELDVILERWIENHRQQRKVSG-TKHNDSDFVDVMLSLAEQGKLSHLQY 294

Query: 191 HVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVKETD 250
             +T IK TCL +ILGG +T+  T+TWA+ LLLNNK+ LKK Q E+D  VGR+R V+++D
Sbjct: 295 DANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSD 354

Query: 251 LKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDPLVW 310
           +KNL YLQAI+KETLRLYPAAPLL   E++EDCTVAGY++P GTRLIVNV K+QRDP V+
Sbjct: 355 IKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVY 414

Query: 311 EDPFEFRPERFLTSQ-KNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGFE 370
            +P EFRPERF+T + K+FDVRGQN + +PFG+GRR CP  S A+Q+++L L+ FLH FE
Sbjct: 415 MEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFE 474

Query: 371 IDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLY 399
           +    +  +DM ES GLT  K TPLEV++ PRL   L+
Sbjct: 475 VKTVLDRPVDMSESPGLTITKATPLEVLINPRLKRELF 511

[Close]

BLAST of Cucsat.G13395 vs. NCBI nr
Match: XP_011652397.1 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN60056.2 hypothetical protein Csa_002536 [Cucumis sativus])

HSP 1 Score: 806 bits (2081), Expect = 1.71e-292
Identity = 395/399 (99.00%), Postives = 397/399 (99.50%), Query Frame = 0


Query: 1   MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVV 60
           MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWM+ELYELWRLNNKGEKVV
Sbjct: 120 MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMEELYELWRLNNKGEKVV 179

Query: 61  VEMKKRLADVTLNTMFKMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWL 120
           VEMKKRLADVTLNTMFKMVIGKKFSS EYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWL
Sbjct: 180 VEMKKRLADVTLNTMFKMVIGKKFSSMEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWL 239

Query: 121 DLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFN 180
           DLGGHKKVMKKTAKI+DEVFDKFLKEHRERINNFGELP AEKDFMDVMISTVEDDGQHFN
Sbjct: 240 DLGGHKKVMKKTAKIMDEVFDKFLKEHRERINNFGELPAAEKDFMDVMISTVEDDGQHFN 299

Query: 181 CHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVKET 240
           CHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVKET
Sbjct: 300 CHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVKET 359

Query: 241 DLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDPLV 300
           DLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDPLV
Sbjct: 360 DLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDPLV 419

Query: 301 WEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGFE 360
           WEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGFE
Sbjct: 420 WEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGFE 479

Query: 361 IDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLYG 399
           IDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLYG
Sbjct: 480 IDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLYG 518

[Close]

BLAST of Cucsat.G13395 vs. NCBI nr
Match: XP_008464769.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo])

HSP 1 Score: 765 bits (1976), Expect = 1.68e-276
Identity = 370/399 (92.73%), Postives = 388/399 (97.24%), Query Frame = 0


Query: 1   MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVV 60
           MMGFSPYSPHWR+VRKIATLELLTNHRV QLQYIRAFE++TWMKELYELWRLNNKGEKVV
Sbjct: 120 MMGFSPYSPHWRHVRKIATLELLTNHRVAQLQYIRAFEIKTWMKELYELWRLNNKGEKVV 179

Query: 61  VEMKKRLADVTLNTMFKMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWL 120
           VEMKKRLAD+TLNT+FKMVIGK+FSS EYGNEKFQ VLIEFFGLFGIFI SDSFPFLSWL
Sbjct: 180 VEMKKRLADITLNTIFKMVIGKRFSSIEYGNEKFQNVLIEFFGLFGIFIPSDSFPFLSWL 239

Query: 121 DLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFN 180
           DLGGH+K MKKTAKIIDEVFDKFLKE RERINNFGE+  AEK FMD+MISTV+DDGQHFN
Sbjct: 240 DLGGHEKAMKKTAKIIDEVFDKFLKERRERINNFGEVEAAEKGFMDIMISTVQDDGQHFN 299

Query: 181 CHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVKET 240
           CHVDTVIKATCLNMILGGFDTTT+TMTWALCLLLNNKE LKKAQVEL+EQVGRERQV+ET
Sbjct: 300 CHVDTVIKATCLNMILGGFDTTTITMTWALCLLLNNKEELKKAQVELNEQVGRERQVEET 359

Query: 241 DLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDPLV 300
           DLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQ+DP+V
Sbjct: 360 DLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQKDPVV 419

Query: 301 WEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGFE 360
           W+DPFEFRPERFLTSQKNFDVRGQ+PQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGFE
Sbjct: 420 WQDPFEFRPERFLTSQKNFDVRGQHPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGFE 479

Query: 361 IDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLYG 399
           IDRPSEELLDMEES GLTSLKKTPLEVVLTPRLPSH+YG
Sbjct: 480 IDRPSEELLDMEESFGLTSLKKTPLEVVLTPRLPSHVYG 518

[Close]

BLAST of Cucsat.G13395 vs. NCBI nr
Match: XP_022935746.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 644 bits (1660), Expect = 2.07e-228
Identity = 310/399 (77.69%), Postives = 357/399 (89.47%), Query Frame = 0


Query: 1   MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELW-RLNNKGEKV 60
           MMGFSPY+PHWR VRKIATLELLTNHR++QLQ+IRAFEV+ WMKEL+++  + N +GEKV
Sbjct: 120 MMGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQRWMKELHKMSIKSNERGEKV 179

Query: 61  VVEMKKRLADVTLNTMFKMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSW 120
            VEMKK LAD+TLNT+FKMVIGK+FS+ ++G E F K LI+FFGLF IF+ SDSFPFLSW
Sbjct: 180 AVEMKKWLADITLNTIFKMVIGKRFSAIQHGTENFPKALIDFFGLFHIFVPSDSFPFLSW 239

Query: 121 LDLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHF 180
           LDLGGH+K MKKTAKIIDE F KFL++HRER N+ GE   AEKDFMDVMI+ VE+DGQHF
Sbjct: 240 LDLGGHEKTMKKTAKIIDEAFHKFLQQHRERRND-GEAETAEKDFMDVMIARVEEDGQHF 299

Query: 181 NCHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVKE 240
           +   DT+IKATCLNMI+GGFDTT VTMTWALCLLLNN+E LKK Q+ELDE+VGR RQVKE
Sbjct: 300 SYDADTIIKATCLNMIVGGFDTTAVTMTWALCLLLNNEETLKKVQLELDEKVGRSRQVKE 359

Query: 241 TDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDPL 300
           +D+KNLPYLQAIVKETLRLYPA PL+ PHES+EDCTVAGYHI KGTRLIVNVQKLQ+DP 
Sbjct: 360 SDVKNLPYLQAIVKETLRLYPAVPLMAPHESVEDCTVAGYHICKGTRLIVNVQKLQKDPR 419

Query: 301 VWEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGF 360
           VWE+P EFRPERFLT QKNFDVRG +PQFIPFGNGRR CPAISFALQ++++TL+NFLHGF
Sbjct: 420 VWEEPSEFRPERFLTGQKNFDVRGLSPQFIPFGNGRRRCPAISFALQVMHITLANFLHGF 479

Query: 361 EIDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLY 398
           EI+R SEELLDMEE +GLTSL+K PLEVVLTPRLP+H+Y
Sbjct: 480 EIERLSEELLDMEEGMGLTSLRKAPLEVVLTPRLPAHIY 517

[Close]

BLAST of Cucsat.G13395 vs. NCBI nr
Match: XP_023535451.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 642 bits (1656), Expect = 8.38e-228
Identity = 309/399 (77.44%), Postives = 356/399 (89.22%), Query Frame = 0


Query: 1   MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELW-RLNNKGEKV 60
           MMGFSPY+PHWR VRKIATLELLTNHR++QLQ+IRAFEV+ WM EL+++  + N +GEKV
Sbjct: 120 MMGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQRWMNELHKMSIKSNERGEKV 179

Query: 61  VVEMKKRLADVTLNTMFKMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSW 120
            VEMKK LAD+TLNT+FKMVIGK+FS+ ++GNE F K LI+FFGLF IF+ SDSFPFLSW
Sbjct: 180 AVEMKKWLADITLNTIFKMVIGKRFSAIQHGNENFPKALIDFFGLFHIFVPSDSFPFLSW 239

Query: 121 LDLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHF 180
           LDLGGH+K MKKTAK+IDE F KFL++HRER N+  E   AEKDFMDVMI+ VE+DGQHF
Sbjct: 240 LDLGGHEKTMKKTAKVIDEAFHKFLQQHRERRND-AEAETAEKDFMDVMIARVEEDGQHF 299

Query: 181 NCHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVKE 240
           +   DTVIKATCLNMI+GGFDTT VTMTWALCLLLNN+EAL K Q+ELDE VGR RQV+E
Sbjct: 300 SYDADTVIKATCLNMIVGGFDTTAVTMTWALCLLLNNEEALNKVQLELDENVGRSRQVEE 359

Query: 241 TDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDPL 300
           +D+KNLPYL+AIVKETLRLYPA PL+ PHES EDC VAGYHI KGTRLIVN+QKLQ+DP 
Sbjct: 360 SDIKNLPYLRAIVKETLRLYPAVPLMAPHESSEDCIVAGYHIYKGTRLIVNLQKLQKDPR 419

Query: 301 VWEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGF 360
           VWE+P EFRPERFLTSQKNFDVRG +PQFIPFGNGRRMCPAISFALQ++++TL+NFLHGF
Sbjct: 420 VWEEPSEFRPERFLTSQKNFDVRGVSPQFIPFGNGRRMCPAISFALQVMHITLANFLHGF 479

Query: 361 EIDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLY 398
           EIDRPSEELLDMEE +GLTSL+K PLEVVLTPRLP+H+Y
Sbjct: 480 EIDRPSEELLDMEEGMGLTSLRKAPLEVVLTPRLPAHIY 517

[Close]

BLAST of Cucsat.G13395 vs. NCBI nr
Match: XP_022976392.1 (cytochrome P450 CYP82D47-like [Cucurbita maxima])

HSP 1 Score: 639 bits (1649), Expect = 1.25e-226
Identity = 308/399 (77.19%), Postives = 356/399 (89.22%), Query Frame = 0


Query: 1   MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELW-RLNNKGEKV 60
           MMGFSPY+PHWR VRKIATLELLTNHR++QLQ+IR FEV+ WMKEL+++  R N +G+KV
Sbjct: 120 MMGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRVFEVQRWMKELHKMSIRSNERGDKV 179

Query: 61  VVEMKKRLADVTLNTMFKMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSW 120
            VEMKK LAD+TLNT+FKMVIGK+FS+ ++G E F K LI+FFGLF IF+ SDSFPFLSW
Sbjct: 180 AVEMKKWLADITLNTIFKMVIGKRFSAIQHGTENFPKALIDFFGLFHIFVPSDSFPFLSW 239

Query: 121 LDLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHF 180
           LDLGGH+K MKKTAK+IDE F KFL++HRER N  G+   AE+DFMDVMIS VE+D QHF
Sbjct: 240 LDLGGHEKTMKKTAKVIDEAFHKFLQQHRERRNE-GQAETAEQDFMDVMISRVEEDEQHF 299

Query: 181 NCHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVKE 240
           +   DTVIKATCLNMI+GGFDTT VTMTWALCLLLNN+EALKK Q+ELDE+VGR  QVKE
Sbjct: 300 SYDADTVIKATCLNMIVGGFDTTAVTMTWALCLLLNNEEALKKVQLELDEKVGRSTQVKE 359

Query: 241 TDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDPL 300
           +D+KNLPYLQAIVKETLRLYPA PL+ PHES+EDCTVAGYHI KGTRLIVNVQKLQ+DP 
Sbjct: 360 SDVKNLPYLQAIVKETLRLYPAVPLMAPHESVEDCTVAGYHICKGTRLIVNVQKLQKDPR 419

Query: 301 VWEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGF 360
           VWE+P EF PERF+TSQKNFDVRG +PQFIPFGNGRRMCPAISFALQ++++TL+NFLHGF
Sbjct: 420 VWEEPNEFCPERFVTSQKNFDVRGLSPQFIPFGNGRRMCPAISFALQVMHITLANFLHGF 479

Query: 361 EIDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLY 398
           EI+RPSEELLDMEE +GLTSL+K PLEVVLTPRLP+H+Y
Sbjct: 480 EIERPSEELLDMEEGMGLTSLRKAPLEVVLTPRLPAHIY 517

[Close]

BLAST of Cucsat.G13395 vs. ExPASy TrEMBL
Match: A0A1S3CMD6 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502577 PE=3 SV=1)

HSP 1 Score: 765 bits (1976), Expect = 8.11e-277
Identity = 370/399 (92.73%), Postives = 388/399 (97.24%), Query Frame = 0


Query: 1   MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVV 60
           MMGFSPYSPHWR+VRKIATLELLTNHRV QLQYIRAFE++TWMKELYELWRLNNKGEKVV
Sbjct: 120 MMGFSPYSPHWRHVRKIATLELLTNHRVAQLQYIRAFEIKTWMKELYELWRLNNKGEKVV 179

Query: 61  VEMKKRLADVTLNTMFKMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSWL 120
           VEMKKRLAD+TLNT+FKMVIGK+FSS EYGNEKFQ VLIEFFGLFGIFI SDSFPFLSWL
Sbjct: 180 VEMKKRLADITLNTIFKMVIGKRFSSIEYGNEKFQNVLIEFFGLFGIFIPSDSFPFLSWL 239

Query: 121 DLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHFN 180
           DLGGH+K MKKTAKIIDEVFDKFLKE RERINNFGE+  AEK FMD+MISTV+DDGQHFN
Sbjct: 240 DLGGHEKAMKKTAKIIDEVFDKFLKERRERINNFGEVEAAEKGFMDIMISTVQDDGQHFN 299

Query: 181 CHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVKET 240
           CHVDTVIKATCLNMILGGFDTTT+TMTWALCLLLNNKE LKKAQVEL+EQVGRERQV+ET
Sbjct: 300 CHVDTVIKATCLNMILGGFDTTTITMTWALCLLLNNKEELKKAQVELNEQVGRERQVEET 359

Query: 241 DLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDPLV 300
           DLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQ+DP+V
Sbjct: 360 DLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQKDPVV 419

Query: 301 WEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGFE 360
           W+DPFEFRPERFLTSQKNFDVRGQ+PQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGFE
Sbjct: 420 WQDPFEFRPERFLTSQKNFDVRGQHPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGFE 479

Query: 361 IDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLYG 399
           IDRPSEELLDMEES GLTSLKKTPLEVVLTPRLPSH+YG
Sbjct: 480 IDRPSEELLDMEESFGLTSLKKTPLEVVLTPRLPSHVYG 518

[Close]

BLAST of Cucsat.G13395 vs. ExPASy TrEMBL
Match: A0A6J1F5K6 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442570 PE=3 SV=1)

HSP 1 Score: 644 bits (1660), Expect = 1.00e-228
Identity = 310/399 (77.69%), Postives = 357/399 (89.47%), Query Frame = 0


Query: 1   MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELW-RLNNKGEKV 60
           MMGFSPY+PHWR VRKIATLELLTNHR++QLQ+IRAFEV+ WMKEL+++  + N +GEKV
Sbjct: 120 MMGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQRWMKELHKMSIKSNERGEKV 179

Query: 61  VVEMKKRLADVTLNTMFKMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSW 120
            VEMKK LAD+TLNT+FKMVIGK+FS+ ++G E F K LI+FFGLF IF+ SDSFPFLSW
Sbjct: 180 AVEMKKWLADITLNTIFKMVIGKRFSAIQHGTENFPKALIDFFGLFHIFVPSDSFPFLSW 239

Query: 121 LDLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHF 180
           LDLGGH+K MKKTAKIIDE F KFL++HRER N+ GE   AEKDFMDVMI+ VE+DGQHF
Sbjct: 240 LDLGGHEKTMKKTAKIIDEAFHKFLQQHRERRND-GEAETAEKDFMDVMIARVEEDGQHF 299

Query: 181 NCHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVKE 240
           +   DT+IKATCLNMI+GGFDTT VTMTWALCLLLNN+E LKK Q+ELDE+VGR RQVKE
Sbjct: 300 SYDADTIIKATCLNMIVGGFDTTAVTMTWALCLLLNNEETLKKVQLELDEKVGRSRQVKE 359

Query: 241 TDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDPL 300
           +D+KNLPYLQAIVKETLRLYPA PL+ PHES+EDCTVAGYHI KGTRLIVNVQKLQ+DP 
Sbjct: 360 SDVKNLPYLQAIVKETLRLYPAVPLMAPHESVEDCTVAGYHICKGTRLIVNVQKLQKDPR 419

Query: 301 VWEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGF 360
           VWE+P EFRPERFLT QKNFDVRG +PQFIPFGNGRR CPAISFALQ++++TL+NFLHGF
Sbjct: 420 VWEEPSEFRPERFLTGQKNFDVRGLSPQFIPFGNGRRRCPAISFALQVMHITLANFLHGF 479

Query: 361 EIDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLY 398
           EI+R SEELLDMEE +GLTSL+K PLEVVLTPRLP+H+Y
Sbjct: 480 EIERLSEELLDMEEGMGLTSLRKAPLEVVLTPRLPAHIY 517

[Close]

BLAST of Cucsat.G13395 vs. ExPASy TrEMBL
Match: A0A6J1IFL6 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476811 PE=3 SV=1)

HSP 1 Score: 639 bits (1649), Expect = 6.05e-227
Identity = 308/399 (77.19%), Postives = 356/399 (89.22%), Query Frame = 0


Query: 1   MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELW-RLNNKGEKV 60
           MMGFSPY+PHWR VRKIATLELLTNHR++QLQ+IR FEV+ WMKEL+++  R N +G+KV
Sbjct: 120 MMGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRVFEVQRWMKELHKMSIRSNERGDKV 179

Query: 61  VVEMKKRLADVTLNTMFKMVIGKKFSSTEYGNEKFQKVLIEFFGLFGIFILSDSFPFLSW 120
            VEMKK LAD+TLNT+FKMVIGK+FS+ ++G E F K LI+FFGLF IF+ SDSFPFLSW
Sbjct: 180 AVEMKKWLADITLNTIFKMVIGKRFSAIQHGTENFPKALIDFFGLFHIFVPSDSFPFLSW 239

Query: 121 LDLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQHF 180
           LDLGGH+K MKKTAK+IDE F KFL++HRER N  G+   AE+DFMDVMIS VE+D QHF
Sbjct: 240 LDLGGHEKTMKKTAKVIDEAFHKFLQQHRERRNE-GQAETAEQDFMDVMISRVEEDEQHF 299

Query: 181 NCHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVKE 240
           +   DTVIKATCLNMI+GGFDTT VTMTWALCLLLNN+EALKK Q+ELDE+VGR  QVKE
Sbjct: 300 SYDADTVIKATCLNMIVGGFDTTAVTMTWALCLLLNNEEALKKVQLELDEKVGRSTQVKE 359

Query: 241 TDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDPL 300
           +D+KNLPYLQAIVKETLRLYPA PL+ PHES+EDCTVAGYHI KGTRLIVNVQKLQ+DP 
Sbjct: 360 SDVKNLPYLQAIVKETLRLYPAVPLMAPHESVEDCTVAGYHICKGTRLIVNVQKLQKDPR 419

Query: 301 VWEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHGF 360
           VWE+P EF PERF+TSQKNFDVRG +PQFIPFGNGRRMCPAISFALQ++++TL+NFLHGF
Sbjct: 420 VWEEPNEFCPERFVTSQKNFDVRGLSPQFIPFGNGRRMCPAISFALQVMHITLANFLHGF 479

Query: 361 EIDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLY 398
           EI+RPSEELLDMEE +GLTSL+K PLEVVLTPRLP+H+Y
Sbjct: 480 EIERPSEELLDMEEGMGLTSLRKAPLEVVLTPRLPAHIY 517

[Close]

BLAST of Cucsat.G13395 vs. ExPASy TrEMBL
Match: A0A6J1CCZ4 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009608 PE=3 SV=1)

HSP 1 Score: 592 bits (1525), Expect = 4.26e-208
Identity = 287/402 (71.39%), Postives = 343/402 (85.32%), Query Frame = 0


Query: 1   MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVV 60
           M G SPY PHWR  R++ATLELLTNHR+++LQ++R  EV+ W+KELY+L   N   EK V
Sbjct: 125 MFGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENE--EKAV 184

Query: 61  VEMKKRLADVTLNTMFKMVIGKKFSST---EYGNEKFQKVLIEFFGLFGIFILSDSFPFL 120
           VEMK    DVTLNT+F+MV+GK+FS+      G+E+ +K L +FFGLFGIFI SD+F FL
Sbjct: 185 VEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQFL 244

Query: 121 SWLDLGGHKKVMKKTAKIIDEVFDKFLKEHRER-INNFGELPVAEKDFMDVMISTVEDDG 180
           SW DLGGH+K MK+TA+++D+ FDKFLKEH+++  +NFGE  + E+DFMDVMIS V+++ 
Sbjct: 245 SWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKNEA 304

Query: 181 QHFNCHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQ 240
           + F+   +TVIKATCLNMILGGFDTTTVTMTWA+ LLLNN+EALKKAQ+ELDEQVGRERQ
Sbjct: 305 ESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQ 364

Query: 241 VKETDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQR 300
           V E+D+KNL YL+AIVKETLRLYP APLL PHESIEDCT  GY+IP GTRLIVNV+KLQR
Sbjct: 365 VNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKLQR 424

Query: 301 DPLVWEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFL 360
           DP VWEDP EFRPERFLTSQ NFDVRGQ+PQFIPFGNGRRMCP ISFALQ+I+LTL+N L
Sbjct: 425 DPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLANLL 484

Query: 361 HGFEIDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLY 398
           HGFEI RPSEELLDMEES+GLTS++KTPLE+VLTPRLP+ +Y
Sbjct: 485 HGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 524

[Close]

BLAST of Cucsat.G13395 vs. ExPASy TrEMBL
Match: A0A6J1FQP6 (cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)

HSP 1 Score: 583 bits (1504), Expect = 4.99e-205
Identity = 290/400 (72.50%), Postives = 339/400 (84.75%), Query Frame = 0


Query: 1   MMGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMKELYELWRLNNKGEKVV 60
           MMG SPYSPHWR+ RKIATLELLTNHR++QLQ+IRA EV+ W  +LYELW +  K EK+V
Sbjct: 119 MMGLSPYSPHWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELW-VKRKDEKLV 178

Query: 61  VEMKKRLADVTLNTMFKMVIGKKFSSTEYGN--EKFQKVLIEFFGLFGIFILSDSFPFLS 120
           VEMK   AD+TLNT+F MVIGK+FS+   G+  E  QK L + F LFG+F+ SDSFPFL 
Sbjct: 179 VEMKNLFADITLNTIFMMVIGKRFSTAFDGHDGEDCQKALRDVFELFGMFVPSDSFPFLW 238

Query: 121 WLDLGGHKKVMKKTAKIIDEVFDKFLKEHRERINNFGELPVAEKDFMDVMISTVEDDGQH 180
           WLD GGH+K MKKTAKI+D  F+KFLKEHR+R +   +L + E+DFMDVMIS V +DG  
Sbjct: 239 WLDFGGHQKRMKKTAKILDGAFEKFLKEHRQRRDRV-KLEMKEEDFMDVMISRVNEDGNL 298

Query: 181 FNCHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVGRERQVK 240
           F+   DTV+KATCLNMILGGFDT TVTMTWAL LLLNN+E+LKKAQ+ELDEQVGRERQV+
Sbjct: 299 FSFDGDTVVKATCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQ 358

Query: 241 ETDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQKLQRDP 300
           E+D+KNLPYLQAIVKETLRLYPAAPLLVPHES+EDCTVAGY+IP GTRLIV+ QKLQ+DP
Sbjct: 359 ESDVKNLPYLQAIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDP 418

Query: 301 LVWEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTLSNFLHG 360
             WE+P+EFRP+RFLTSQKN DVRGQ+PQ IPFGNGRRMCP ISFALQ+++LTL N LHG
Sbjct: 419 SFWENPYEFRPKRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHG 478

Query: 361 FEIDRPSEELLDMEESIGLTSLKKTPLEVVLTPRLPSHLY 398
           F I R SEE LDMEES GLTS++K PLEVVL+PRLP+H+Y
Sbjct: 479 FNIGRMSEEPLDMEESFGLTSVRKAPLEVVLSPRLPAHVY 516

The following BLAST results are available for this feature:

BLAST of Cucsat.G13395 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZ46	1.0e-109	50.00	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]
A0A0N7F297	2.6e-105	50.87	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]
O49858	3.4e-105	48.02	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]
O49394	7.7e-105	48.89	Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=...	[more]
O49396	7.2e-103	49.25	Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3	[more]

BLAST of Cucsat.G13395 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652397.1	1.71e-292	99.00	cytochrome P450 CYP82D47 [Cucumis sativus] >KGN60056.2 hypothetical protein Csa_...	[more]
XP_008464769.1	1.68e-276	92.73	PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo]	[more]
XP_022935746.1	2.07e-228	77.69	cytochrome P450 CYP82D47-like [Cucurbita moschata]	[more]
XP_023535451.1	8.38e-228	77.44	cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo]	[more]
XP_022976392.1	1.25e-226	77.19	cytochrome P450 CYP82D47-like [Cucurbita maxima]	[more]

BLAST of Cucsat.G13395 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CMD6	8.11e-277	92.73	cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502577 PE=3 SV=1	[more]
A0A6J1F5K6	1.00e-228	77.69	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442570 PE=3...	[more]
A0A6J1IFL6	6.05e-227	77.19	cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476811 PE=3 S...	[more]
A0A6J1CCZ4	4.26e-208	71.39	cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009608 PE=...	[more]
A0A6J1FQP6	4.99e-205	72.50	cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 22..163 e-value: 7.0E-11 score: 41.5
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..176 e-value: 2.3E-17 score: 64.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 4..163
None	No IPR available	PANTHER	PTHR24281	STEROID 21-HYDROXYLASE-RELATED	coord: 1..174
None	No IPR available	PANTHER	PTHR24281:SF48	CYTOCHROME P450	coord: 1..174

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G13395.T1	Cucsat.G13395.T1	mRNA
Cucsat.G13395.T2	Cucsat.G13395.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen