Cucsat.G13250.T4 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G13250.T4
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	aquaporin NIP2-1-like
Location	ctg1838: 3149337 .. 3152368 (+)
RNA-Seq Expression	Cucsat.G13250.T4
Synteny	Cucsat.G13250.T4

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCCGCCCGAGTTCTCACGAAAGGTATGGATTTCTAGTTAATTTTGTTCTTGGTTGAATTTGAAGCGATTGGTTTGTGATCGGAGGAATTTTTGTGGTGGTTTAGCTTGTGGCGGAGGTGATCGCTACGTATTTGCTTGTGTTTGTGTCGTGTGGTGTGGCGGCATTGAGTGGGAGTGATGAACCGGTAGTGACGAAGCTTGGAGCTTCAATTACCTGTGGATTGATTGTGACGGTGATGATTTACTCCGTCGGACATATCTCCGGCGCTCATATGAATCCAGCGGTTACTATTGCTTTTGCTGCTGTTCGGCGATTTCCATGGAGACAGGTCATCTTCTTCTTCCTCCTCTGTTTTTCTCCCCAATTTTTATGCCAATTTACCATATTATCCTTTCGCTTTTTCATTTGTTATTTTTATGTTTTCTACAGATATTTTCTATTTTGAGAAAAGCCGTGCCAAATATTTAATTCACTTTTTTGCCTTTTTTTTTCTTCTTTTTTTAAGTTTTGATATTTAGTTTAAGAGTGGAAACCTATACTTAAAAAAGTTTCGAAATATTACATTTGTACCATTTAATTTTTTTATCATGTTATTTATTTTTAATTCTTTATTTTAAATTTAGTTTTCATTTACTCCCTACATTTCAATATTTAGTTTCAATTTTTGTTTGTTAATAGCACTGCTTGTTTTGTTATTCATACTTCAATAATTAAGTTCAAATGATTCTATCATAATTTTTTTACTTATAATTAATTAATTAATTTTTAGATATTAAAATTGATGTTGAGTATTTAAACCATATAAAATTCAAAAATCATGCTACATTTTCATGACTTAAAATACAGGAATTAAAAAGTTCCTTTTTTTTTTCTAAATGTAAAAAGAAAAAAAAAAGTTTTTCTTTCTTTCTTTTCAACATTAAAAGCATGCTTAGATATTGGTGGTTGAGTTGATAAGTGTACTGAGAATAGATGTGTATTAGGTAATATAATAAAAATGTCCAATAAAAAGACAAAACAATGTCTCCTTTCTTTTAACATTTAGAAAACAAAATCAAATTTATCATTATTTTTTTCAAGTTTACTAAATTAAAAATAGTAGAATTCTTATTTTAACTTTGGTACAAAATATTTGAACAATTATACACCGACAAATAATCACCCTGAATCTAGAGCTTTGGATTTCTAGTGCACTCTCTCATTTTCGAAAAATAAAATAAAATTATCAACATATTCTCTCTCTCTTTTTTTTTTTTCTTTTCAGAATACATACTCTTTTTTGCTCTATTTCACTCTTTTTTAAAAAATTTAAAAATTAAGAATATATACTCTTTTTCTTTTTCTTTTTTAGATTATATACTATCTTAATCTCTCTTTTTTTTCTGAAACTGTATTAAAAATTAAGATTATATATACTCTCTTACTTTTTTTTTTATTTAAGAATTATAATCTCTTACTTAAGAATTAAGAAAAAGGAACCCCCAATCAAATCAACAGTGATGGTGATATATATTATAAATGTCTATTTTATTGGACGGGCGGAAATTAATTATAATTTTTTTAAAATTGGAAATTTAGTGGTAATGATGTATGAGCTGCGCCACTTCATCAAAAAGAACAGTAAAAAATTATAAAAAAAGGGATGGAAAAGTGCAATTTATGCCACCTGAGAAGTGACGTGGATGAAGTGTACGGAAGATATTTCGATAAGTGTCCCCACCAGTTTGGCAGATATTATATATAAAAAGACTATTGGCAGATACTTTCTAAGGAAAGCAGCCTTAATTGCTTTTTAATTCACCAAAAGTCATCTTAATTGAGAATATGCTTTTCCATCTTTTCCAGTTTAGACCTTTCAAACCTTTTTTCTTTTTAACTCTTTTTCTATTTTATTTTCATATATATTTTTTACATGACTTAGTAATAATTGATGTATACTCTTATGGTTCGTGAATTGAATTTTATGCGGTTGATGCAGGTTCCACTTTATGCAGCAGCTCAATTAAGTGGAGCTACGTCTGCAGCCTTCACACTACGCATATTAATGGATCCAATTCAAGATTTAGGTACTACTTCACCACATGGACCGGCTTTGAAAGCACTTGTTATGGAGATTGTTGTCTCATTTTGTATGATGTTTGTCACTTCTGCGGTTGCCACCGACACCAAAGCTGTAAGAGCATCTTATTGTTGTTTACCTCTTACTCACAATCTTCTCTTAATTTCCTTTCGCTAAGGTTCACTGCTTTTTTATATGTAATTTTTGGCAGATAGGAGAGCTTGGAGGCGTAGCTGTGGGGTCAGCTGTATGTATCTCGTCCATCTTTGCTGGGTAAGCAAAGCAATTATAATTGTTTCTTTTTTTGGCTTTTTGTTCTTAGCTGGAAGTTTTGTGGGTATCTGGAAAAATCTTAAACAAGATTTTGTTTTGATTGAATTCCAAATTTGGACCCTATAGTTTGGAGAAAATTTGTTTATTTATGAGATTTTATCAAACTATAAAGCTGGGGTTAACTATGTGTAGGCCAATATCGGGTGGATCGATGAACCCAGCAAGATCAATAGGACCAGCCATTGCAAGTTCACGTTACGAAGGAATTTGGGTGTACATGATCGGTCCAGTTACAGGAACCTTGCTTGCATCATTTTCATATAATTTCATACGAGCCACTGAAAAACACACCCATTCACTCTCGTTACATTGACATCCAATCGATGGGATCATGCAGCTGGAACTTCAAGTCATACCAAAATCAACTAGTTCAATTATTGTATAAATTAATGTGTAGCTCCTATAATAAAAGTTGTAGTTTAAGTTGTAGCTTTAGAAAAACTTAAGGTAAGGTTAGCTTTAATTGTCTTGCAATTTATTTGTTTTTTTAGTTTCGAGAGAAGAATGAATCTGAAATAGAGATTCATTTTTCTTTTTATTACTCTTGTTATTTTACAAGTGTGAGTTTTGATCTGATTTTTTTTACAATAATCAATCATAAAATTGGTTCTTCTTTTCAAGTTAATTCTCTCATATAT

Coding sequence (CDS)

ATGATTTACTCCGTCGGACATATCTCCGGCGCTCATATGAATCCAGCGGTTACTATTGCTTTTGCTGCTGTTCGGCGATTTCCATGGAGACAGGTTCCACTTTATGCAGCAGCTCAATTAAGTGGAGCTACGTCTGCAGCCTTCACACTACGCATATTAATGGATCCAATTCAAGATTTAGGTACTACTTCACCACATGGACCGGCTTTGAAAGCACTTGTTATGGAGATTGTTGTCTCATTTTGTATGATGTTTGTCACTTCTGCGGTTGCCACCGACACCAAAGCTATAGGAGAGCTTGGAGGCGTAGCTGTGGGGTCAGCTGTATGTATCTCGTCCATCTTTGCTGGGCCAATATCGGGTGGATCGATGAACCCAGCAAGATCAATAGGACCAGCCATTGCAAGTTCACGTTACGAAGGAATTTGGGTGTACATGATCGGTCCAGTTACAGGAACCTTGCTTGCATCATTTTCATATAATTTCATACGAGCCACTGAAAAACACACCCATTCACTCTCGTTACATTGA

Protein sequence

MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH

Homology

[Close]

BLAST of Cucsat.G13250.T4 vs. ExPASy Swiss-Prot
Match: Q19KC1 (Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2)

HSP 1 Score: 221.1 bits (562), Expect = 1.0e-56
Identity = 108/171 (63.16%), Postives = 138/171 (80.70%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIY+VGHISGAHMNPAVT+AFA  R FPW QVP Y AAQ +G+  A+F L+ ++ PI  L
Sbjct: 93  MIYAVGHISGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTGSICASFVLKAVLHPIAVL 152

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTT+P GP   +LV+EI+V+F MMFVT AVATDT+A+GEL G+AVGSAVCI+SIFAG +S
Sbjct: 153 GTTTPTGPHWHSLVIEIIVTFNMMFVTLAVATDTRAVGELAGLAVGSAVCITSIFAGAVS 212

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTH 172
           GGSMNPAR++GPA+AS+ Y G+W+Y +GPV GTL  +++Y +IR  E  +H
Sbjct: 213 GGSMNPARTLGPALASNLYTGLWIYFLGPVLGTLSGAWTYTYIRFEEAPSH 263

[Close]

BLAST of Cucsat.G13250.T4 vs. ExPASy Swiss-Prot
Match: Q6Z2T3 (Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.7e-56
Identity = 105/164 (64.02%), Postives = 137/164 (83.54%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIY+VGHISGAHMNPAVT+AFA  R FPW QVP Y AAQ +GA  A+F L+ ++ P+  +
Sbjct: 95  MIYAVGHISGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTGAICASFVLKAVIHPVDVI 154

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTT+P GP   +LV+E++V+F MMFVT AVATDT+A+GEL G+AVGSAVCI+SIFAG IS
Sbjct: 155 GTTTPVGPHWHSLVVEVIVTFNMMFVTLAVATDTRAVGELAGLAVGSAVCITSIFAGAIS 214

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIR 165
           GGSMNPAR++GPA+AS++++G+W+Y +GPV GTL  +++Y FIR
Sbjct: 215 GGSMNPARTLGPALASNKFDGLWIYFLGPVMGTLSGAWTYTFIR 258

[Close]

BLAST of Cucsat.G13250.T4 vs. ExPASy Swiss-Prot
Match: Q67WJ8 (Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 2.2e-56
Identity = 109/167 (65.27%), Postives = 135/167 (80.84%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIY+ GHISGAHMNPAVT++FA  R FPW QVP Y AAQ +GA  AAF LR ++ PI+ L
Sbjct: 98  MIYATGHISGAHMNPAVTLSFAFFRHFPWIQVPFYWAAQFTGAMCAAFVLRAVLYPIEVL 157

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTT+P GP   ALV+EIVV+F MMFVT AVATD++A+GEL G+AVGSAVCI+SIFAGP+S
Sbjct: 158 GTTTPTGPHWHALVIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITSIFAGPVS 217

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATE 168
           GGSMNPAR++ PA+AS+ Y G+W+Y +GPV GTL  ++ Y +IR  E
Sbjct: 218 GGSMNPARTLAPAVASNVYTGLWIYFLGPVVGTLSGAWVYTYIRFEE 264

[Close]

BLAST of Cucsat.G13250.T4 vs. ExPASy Swiss-Prot
Match: Q9AT74 (Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 3.2e-55
Identity = 105/167 (62.87%), Postives = 133/167 (79.64%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIY+ GHISGAHMNPAVT++FA  R FPW QVP Y AAQ +GA  AAF L+ ++ PI  +
Sbjct: 101 MIYATGHISGAHMNPAVTLSFACFRHFPWIQVPFYWAAQFTGAMCAAFVLKAVLQPIAVI 160

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTT+P GP   AL +EIVV+F MMFVT AVATD++A+GEL G+AVGSAVCI+SIFAGP+S
Sbjct: 161 GTTTPSGPHWHALAIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITSIFAGPVS 220

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATE 168
           GGSMNPAR++ PA+AS+ + G+W+Y +GPV GTL  ++ Y +IR  E
Sbjct: 221 GGSMNPARTLAPAVASNVFTGLWIYFLGPVVGTLSGAWVYTYIRFEE 267

[Close]

BLAST of Cucsat.G13250.T4 vs. ExPASy Swiss-Prot
Match: Q9ATN2 (Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 4.2e-55
Identity = 109/178 (61.24%), Postives = 138/178 (77.53%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIY+ GHISGAHMNPAVT++FA  R FPW QVP Y AAQ +GA  AAF L+ ++ PI  +
Sbjct: 98  MIYATGHISGAHMNPAVTLSFACFRHFPWIQVPFYWAAQFTGAMCAAFVLKAVLHPIAVI 157

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTT+P GP   AL++EIVV+F MMFVT AVATD++A+GEL G+AVGSAVCI+SIFAGP+S
Sbjct: 158 GTTTPSGPHWHALLIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITSIFAGPVS 217

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATE----KHTHSLS 175
           GGSMNPAR++ PA+AS+ + G+W+Y +GPV GTL  ++ Y +IR  E    K T  LS
Sbjct: 218 GGSMNPARTLAPAVASNVFTGLWIYFLGPVIGTLSGAWVYTYIRFEEAPAAKDTQRLS 275

[Close]

BLAST of Cucsat.G13250.T4 vs. NCBI nr
Match: APT69294.1 (LSi6 [Cucumis sativus var. sativus] >KGN59571.1 hypothetical protein Csa_000858 [Cucumis sativus])

HSP 1 Score: 342 bits (878), Expect = 3.04e-117
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL
Sbjct: 86  MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 145

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS
Sbjct: 146 GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 205

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH 176
           GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH
Sbjct: 206 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH 261

[Close]

BLAST of Cucsat.G13250.T4 vs. NCBI nr
Match: NP_001292702.1 (aquaporin NIP2-1-like [Cucumis sativus] >ACU29604.1 Si transport-like protein 2 [Cucumis sativus])

HSP 1 Score: 341 bits (874), Expect = 1.24e-116
Identity = 175/176 (99.43%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIYS GHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL
Sbjct: 86  MIYSAGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 145

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS
Sbjct: 146 GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 205

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH 176
           GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH
Sbjct: 206 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH 261

[Close]

BLAST of Cucsat.G13250.T4 vs. NCBI nr
Match: NP_001380719.1 (aquaporin NIP2-2 [Cucumis melo])

HSP 1 Score: 335 bits (858), Expect = 3.38e-114
Identity = 169/176 (96.02%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIY+VGHISGAHMNPAVTIAFAAVRRFPW+QVPLYAAAQLSGATSAAFTLRIL+DPIQDL
Sbjct: 86  MIYAVGHISGAHMNPAVTIAFAAVRRFPWKQVPLYAAAQLSGATSAAFTLRILLDPIQDL 145

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGG+AVGSAVCISSIFAGPIS
Sbjct: 146 GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGIAVGSAVCISSIFAGPIS 205

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH 176
           GGSMNPARSIGPAIASSRYEGIWVYMIGP+TGTLLA+FSYNFIRATEKHTHSLS H
Sbjct: 206 GGSMNPARSIGPAIASSRYEGIWVYMIGPITGTLLAAFSYNFIRATEKHTHSLSSH 261

[Close]

BLAST of Cucsat.G13250.T4 vs. NCBI nr
Match: XP_038906096.1 (aquaporin NIP2-2-like [Benincasa hispida])

HSP 1 Score: 310 bits (793), Expect = 3.25e-104
Identity = 155/175 (88.57%), Postives = 166/175 (94.86%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIY+VGHISGAHMNPAVT AFAAVRRFPW QVPLYAAAQLSGAT+AAFTLRIL+DPIQDL
Sbjct: 92  MIYAVGHISGAHMNPAVTFAFAAVRRFPWNQVPLYAAAQLSGATTAAFTLRILLDPIQDL 151

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTTSP GP  KAL+MEIVVSFCMMFVTSAVATDTKA+GEL G+AVGSAVCI+SIFAGPIS
Sbjct: 152 GTTSPSGPIFKALLMEIVVSFCMMFVTSAVATDTKAVGELAGIAVGSAVCITSIFAGPIS 211

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSL 175
           GGSMNPARSIGPAIASSRYEGIWVY++GPV GTLL +FSYNFIRATEKH+ SLSL
Sbjct: 212 GGSMNPARSIGPAIASSRYEGIWVYLVGPVAGTLLGAFSYNFIRATEKHSRSLSL 266

[Close]

BLAST of Cucsat.G13250.T4 vs. NCBI nr
Match: XP_023528272.1 (aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302 bits (774), Expect = 2.28e-101
Identity = 150/175 (85.71%), Postives = 165/175 (94.29%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIY+VGHISGAHMNPAVT AFAAVRRFPW+QVPLYAAAQLSGATSAAFTLRIL+DPI++L
Sbjct: 89  MIYAVGHISGAHMNPAVTFAFAAVRRFPWKQVPLYAAAQLSGATSAAFTLRILLDPIKEL 148

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTTSP GP  KAL++EIVVSFCMMFVTSAVATDTKAIGEL G+AVGS+VCI+SIFAGPIS
Sbjct: 149 GTTSPSGPEFKALIVEIVVSFCMMFVTSAVATDTKAIGELAGIAVGSSVCITSIFAGPIS 208

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSL 175
           GGSMNPARSIGPAIASS YEGIWVY++GPVTGTLL ++SYNFIR +EKH H LSL
Sbjct: 209 GGSMNPARSIGPAIASSHYEGIWVYLVGPVTGTLLGAWSYNFIRTSEKHGHQLSL 263

[Close]

BLAST of Cucsat.G13250.T4 vs. ExPASy TrEMBL
Match: A0A1L7B7J9 (LSi6 OS=Cucumis sativus var. sativus OX=869827 GN=LSi6 PE=2 SV=1)

HSP 1 Score: 342 bits (878), Expect = 1.47e-117
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL
Sbjct: 86  MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 145

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS
Sbjct: 146 GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 205

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH 176
           GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH
Sbjct: 206 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH 261

[Close]

BLAST of Cucsat.G13250.T4 vs. ExPASy TrEMBL
Match: A0A0A0LHR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G826650 PE=3 SV=1)

HSP 1 Score: 342 bits (878), Expect = 1.47e-117
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL
Sbjct: 86  MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 145

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS
Sbjct: 146 GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 205

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH 176
           GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH
Sbjct: 206 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH 261

[Close]

BLAST of Cucsat.G13250.T4 vs. ExPASy TrEMBL
Match: G8XUV4 (Si transport-like protein 2 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 341 bits (874), Expect = 5.99e-117
Identity = 175/176 (99.43%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIYS GHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL
Sbjct: 86  MIYSAGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 145

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS
Sbjct: 146 GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 205

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH 176
           GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH
Sbjct: 206 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH 261

[Close]

BLAST of Cucsat.G13250.T4 vs. ExPASy TrEMBL
Match: A0A1S3B8P6 (aquaporin NIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103487003 PE=3 SV=1)

HSP 1 Score: 335 bits (858), Expect = 1.64e-114
Identity = 169/176 (96.02%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIY+VGHISGAHMNPAVTIAFAAVRRFPW+QVPLYAAAQLSGATSAAFTLRIL+DPIQDL
Sbjct: 86  MIYAVGHISGAHMNPAVTIAFAAVRRFPWKQVPLYAAAQLSGATSAAFTLRILLDPIQDL 145

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGG+AVGSAVCISSIFAGPIS
Sbjct: 146 GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGIAVGSAVCISSIFAGPIS 205

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSLH 176
           GGSMNPARSIGPAIASSRYEGIWVYMIGP+TGTLLA+FSYNFIRATEKHTHSLS H
Sbjct: 206 GGSMNPARSIGPAIASSRYEGIWVYMIGPITGTLLAAFSYNFIRATEKHTHSLSSH 261

[Close]

BLAST of Cucsat.G13250.T4 vs. ExPASy TrEMBL
Match: A0A172B0F5 (Silicon transporter 3 OS=Cucurbita ficifolia OX=37645 GN=Lsi3 PE=2 SV=1)

HSP 1 Score: 301 bits (770), Expect = 4.49e-101
Identity = 149/175 (85.14%), Postives = 164/175 (93.71%), Query Frame = 0


Query: 1   MIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDL 60
           MIY+VGHISGAHMNPAVT AFAAV+RFPW+QVPLYAAAQLSGATSAAFTLRIL+DPI++L
Sbjct: 89  MIYAVGHISGAHMNPAVTFAFAAVKRFPWKQVPLYAAAQLSGATSAAFTLRILLDPIKEL 148

Query: 61  GTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPIS 120
           GTTSP GP  KAL++EIVVSFCMMFVTSAVATDTKAIGEL G+AVGS+VCI+SIFAGPIS
Sbjct: 149 GTTSPSGPEFKALIVEIVVSFCMMFVTSAVATDTKAIGELAGIAVGSSVCITSIFAGPIS 208

Query: 121 GGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATEKHTHSLSL 175
           GGSMNPARSIGPAIASS YEGIWVY++GPV GTLL ++SYNFIR TEKH H LSL
Sbjct: 209 GGSMNPARSIGPAIASSHYEGIWVYLVGPVAGTLLGAWSYNFIRTTEKHGHQLSL 263

The following BLAST results are available for this feature:

BLAST of Cucsat.G13250.T4 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q19KC1	1.0e-56	63.16	Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2	[more]
Q6Z2T3	1.7e-56	64.02	Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1	[more]
Q67WJ8	2.2e-56	65.27	Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1	[more]
Q9AT74	3.2e-55	62.87	Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1	[more]
Q9ATN2	4.2e-55	61.24	Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1	[more]

BLAST of Cucsat.G13250.T4 vs. NCBI nr


Match Name	E-value	Identity	Description
APT69294.1	3.04e-117	100.00	LSi6 [Cucumis sativus var. sativus] >KGN59571.1 hypothetical protein Csa_000858 ...	[more]
NP_001292702.1	1.24e-116	99.43	aquaporin NIP2-1-like [Cucumis sativus] >ACU29604.1 Si transport-like protein 2 ...	[more]
NP_001380719.1	3.38e-114	96.02	aquaporin NIP2-2 [Cucumis melo]	[more]
XP_038906096.1	3.25e-104	88.57	aquaporin NIP2-2-like [Benincasa hispida]	[more]
XP_023528272.1	2.28e-101	85.71	aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G13250.T4 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1L7B7J9	1.47e-117	100.00	LSi6 OS=Cucumis sativus var. sativus OX=869827 GN=LSi6 PE=2 SV=1	[more]
A0A0A0LHR6	1.47e-117	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G826650 PE=3 SV=1	[more]
G8XUV4	5.99e-117	99.43	Si transport-like protein 2 OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]
A0A1S3B8P6	1.64e-114	96.02	aquaporin NIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103487003 PE=3 SV=1	[more]
A0A172B0F5	4.49e-101	85.14	Silicon transporter 3 OS=Cucurbita ficifolia OX=37645 GN=Lsi3 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 75..93 score: 22.08 coord: 106..128 score: 40.01 coord: 31..50 score: 34.45 coord: 143..163 score: 33.09
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 2..160 e-value: 5.8E-44 score: 150.5
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10	Glycerol uptake facilitator protein.	coord: 1..174 e-value: 2.9E-52 score: 179.0
IPR023271	Aquaporin-like	SUPERFAMILY	81338	Aquaporin-like	coord: 1..170
None	No IPR available	PANTHER	PTHR45724:SF30	MAJOR INTRINSIC PROTEIN, AQUAPORIN-LIKE PROTEIN-RELATED	coord: 1..175
IPR034294	Aquaporin transporter	PANTHER	PTHR45724	AQUAPORIN NIP2-1	coord: 1..175
IPR022357	Major intrinsic protein, conserved site	PROSITE	PS00221	MIP	coord: 12..20

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G13250	Cucsat.G13250	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13250.T4.E1	Cucsat.G13250.T4.E1	exon
Cucsat.G13250.T4.E2	Cucsat.G13250.T4.E2	exon
Cucsat.G13250.T4.E3	Cucsat.G13250.T4.E3	exon
Cucsat.G13250.T4.E4	Cucsat.G13250.T4.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13250.T4.C1	Cucsat.G13250.T4.C1	CDS
Cucsat.G13250.T4.C2	Cucsat.G13250.T4.C2	CDS
Cucsat.G13250.T4.C3	Cucsat.G13250.T4.C3	CDS
Cucsat.G13250.T4.C4	Cucsat.G13250.T4.C4	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13250.T4	Cucsat.G13250.T4-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016020	membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0015267	channel activity