Cucsat.G1287.T4 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G1287.T4
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
Location	ctg1: 10912738 .. 10916357 (+)
RNA-Seq Expression	Cucsat.G1287.T4
Synteny	Cucsat.G1287.T4

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AATAGAAAAGGGAAAATCAGCACGTGGGAACCCAAATAATAATACTCCGCCAAATCTCCTTCCATTTCAATTCAATCCAAATTCTTCTTCTTCTTCTTCGCTCTTCTTCATCCTCCTTCAAATTCTTAACAATTGCAGTAATTTCCATTTCTTTTCCTTTGATCTTTTACCAGCAACTCGCTTAGTGATGTCGATTCTTACGATCGGCCGGAGGAGGATTTCGTCGACGAATGCTTGGGCCACCGGTCGCCGGTTACTCTCTCCCTTCACCTCTCAATCTCCTTCTCCTTCAATTTCTCAGTTTCCGTCGCAGCAGACGCCCGAATCCCTTCCACACCCATTTCCTCCGCTTGGATCCCCCAGTTCCGGTCGGGCATGGCTTCATTCACATGCTAATCGTTTCGAGGTGAGTTCCACTCTGGTTGTCGTTTTCCTTTTGTTGTTGTTAGCGTTGGTTCATCTGAATTTTAAGACGTAAGCAAGTTCTATTTCAGTTTTTTTTTTCTTTTTTTCTTTTTCCGGATTTTGAGCTAGCAGCGCTTTCGATGATTCAATGTGTTTGTTCTGCACTAATCTATTCATAATTGTAGAGATCTTCTCCTTGTAACAATCGGGAGAAATTTAGAAATTAGGTGATTGTGAATGTAATCGTGGAATTGGTTCTTCATGCAGGCTAGGATAAAAGGCGTTTCGAAGATATGTCGCTTTTCTAGCCAGGCTTTAGACGGTCTTCCACTTTTTAGGTTGGTCGATATACCTTTGGCTCAAACTGGTGAAGGAATTGCAGAATGTGAGCTTCTTAAATGGTTTGTGCAAGAGGTAAAACCAACAATACTTCTTTCTTTCTTTCATTGTTGGTCCTTTTGTGAAAATGAATGAGGCTTGGTTGATTTCTTCAATCACTTATGTACTGTTCCAAGCTCAAACTGCTGCAATGTGGCTGTCATGCGAGAGCTGTTTGTGTTGGATCTCTGTCTGGTTTACGGAGGATGCAGGATCAGTTCAAGTGATTTCTATTCTGTCAGTTGATTGATTGGACAATTTCTTATTAAAATATAGATGGATGTTCATATTTAATCTTTTGGCAGGGGGATGAAGTTGAGGAGTTTCAACCACTTTGTGAAGTTCAGAGTGACAAAGCAACAATTGAAATAACTAGTCGCTACAAGGGAAAAGTTGGTCAACTTTTATATGTTCCGGGGGACATTGTTAAGGTGATATTTCATCACCCAATTGTTAAGAATATGAACTCATGGAAACTTTTGCAGTTTATGAATGGAGGGTAGAAAGAAATGTGAATGTCTATTTTCGGGGAAATTATTAAGTTGTCAATATATCAAATGAAAAGGTCACTAAACTTTTTGTTTCCTTTACACTGCAATGTTGTAGCTTGTAGGCTTCTTGCTCAAGTTTGATTGTTATATGTTATCGTTTTCAACCTTGATTACTGCTTAGGAAAAGCATTTTTCATTCATGCAGAAGCACACTAGTGGTAAATTATCGTAATATCACATTCTAGAGTTGGAAGAGAAAAGACACTAATGTTATGTCAAACGTTATCTCAGGTTGGAGAAACTCTTTTAAAAGTACACGTCGAGGGTTTCGAGGATGAGATCCAGGTTTCAGGGTTAACTGAAGGGCATTTGGCAAAACCCGAAGTAAAAGAGTCCCAACAGGACAAAAGCAAAAACTGTGGAGTTCTGTCCACACCTCCTGTTCGGGATCTTGCAAAAGAATATGGTATAGACATAAATGATGTCAGTGGAAGTGGTCCAGATGGAAGGGTATTAAAAGAAGACGTTCTTCAGTATGCTGTCAAAAAAGGAATTCTTGAAGACCATGTTTCTTCTGCTGCTAGTTTTAGGGTGCAATTTGATGAATCAGAGACTCACACTCATGCACCAGATGGAGTTATGTGGACCTACGAGGATAAGAAAGTTCAATTGAGGTATTATTCGTAAGGAGCTATTAAGTAGCCAAGAGTGAGTACTTGTCTAAATGAACGTAGTCATATCTTTTCATCATTTAAACAACTCCTGTCCTCTTGGTTTTTCTTGGAATAACTGAAGTGCACCTTGAAGTTCTTTTCAACTAGCAAAATAATTATTTCCTTTGCTATGTTCTAATTGTGTATTATTTGGTTGTGCAATTTATTCCATTTAGAGTTGTATGCAATTGATATGTGTTTTGTTTGCAGAGGATTCCAACGAGCAATGGTTAAATCCATGGCCATTGCAGCAAAGGTTCCCCATTTTCACTACGTAGAGGAGATCAATTGTGATGCACTTCTGGAGCTTAAAGCATCTTTCCAAGGAAATACTACCGAACCAAATGTCAAACACACGTCTCTTCCCTTATTGATAAAGTCCCTTTCTATGGCAATGAGCAAATACCCAATGCTAAATAGTTGCTTCAATGAAGATTCATTTGAGGTCACTCTCAAAGGTATGCAGTAAAGAGAGATGTTTGAATTGAATACATTGTAGAATATAATCCCCCAGATTAGGTCGTGTTCTCTTACTGTTTGCTGCTATGCCTAAGATGAAATCTTTCCTTGTTCTTATTTGAATCACAGTTACATTTACTTTTGCTTGCTGTAGGCTCCCATAATATTGGAATCGCCATGGCTACTCCACATGGTTTGGTTGTGCCAAATATAAAGAATGTTCAGTCCCTTTCTGTTTTGGAGGTTTGTAAATCACCGCCCACACATTTTTGCTTAAACGTAACATGGTCATCTAAAAACTTTTTAGTTTCTTAATTGGGTTAGGAGATTGAATACTCATCTTATGAAGCTTTCTTGTTTCTTAAAACAATTGCCATCATTTGCCAATGTTGCTAGCATGGCTTCCCTGTCACTAAAGACTTATATTTCAAATACATTTGCTTAGATCACAAAGGAGCTATCACGCTTACAACTACTAGCAATGGAAAACAAGCTCAGTCCTGGGGACATATCCGGTGGCACAATAACCTTAAGCAATATCGGAGCCATTGGTGGAAAATACGGTTCTCCTTTGCTCAACTTGCCTGAAGTATCCATCATCGCAATTGGCCGAATAAAAAAAGTTCCACAGATTGCCGATGATGGGAGCGTGTATCCATCATCAATCATGACGGTGAGCAACACAAAAATGTAAAGTCCATCTTATATTTATTATTGACTGACCAACCTTCCCTTCTATTTGGTCAGGTGAACATTGGTGCAGACCATAGAGTTCTGGATGGAGCAACAGTGGCAAGGTTTTGTAACGAGTGGAAGCGATTCATTGAAAATCCAGAGCTTCTGATATTGCATATGAGATGAGAGAAAGTAAATGTATCTGTATCCCCCCATTTGATATAATAAAATGAAAGGGGCCGCCACAAAAAGAAAAAAAGAACGAAATCTTACTTTCAAATTTCATACTGATCGTCGCTCTTTGGATAAGAAGAAAGAAAGAAAGAAGGCTCAATGGTTACGTGACGTAGTCAACTTTGAAAGCTAGATAATGGATTTTGACAAACAGCTCTATGGATTTTGAAAAACAGCTTCTACAAATTTTACGAACTTTCAATTCAAAAGTAACTTATTCATTTAACTTTTAATAAAGTATTTTATTAATGAATTAACTTTTTAA

Coding sequence (CDS)

ATGTGTTTTGTTTGCAGAGGATTCCAACGAGCAATGGTTAAATCCATGGCCATTGCAGCAAAGGTTCCCCATTTTCACTACGTAGAGGAGATCAATTGTGATGCACTTCTGGAGCTTAAAGCATCTTTCCAAGGAAATACTACCGAACCAAATGTCAAACACACGTCTCTTCCCTTATTGATAAAGTCCCTTTCTATGGCAATGAGCAAATACCCAATGCTAAATAGTTGCTTCAATGAAGATTCATTTGAGGTCACTCTCAAAGGCTCCCATAATATTGGAATCGCCATGGCTACTCCACATGGTTTGGTTGTGCCAAATATAAAGAATGTTCAGTCCCTTTCTGTTTTGGAGATCACAAAGGAGCTATCACGCTTACAACTACTAGCAATGGAAAACAAGCTCAGTCCTGGGGACATATCCGGTGGCACAATAACCTTAAGCAATATCGGAGCCATTGGTGGAAAATACGGTTCTCCTTTGCTCAACTTGCCTGAAGTATCCATCATCGCAATTGGCCGAATAAAAAAAGTTCCACAGATTGCCGATGATGGGAGCGTGTATCCATCATCAATCATGACGGTGAACATTGGTGCAGACCATAGAGTTCTGGATGGAGCAACAGTGGCAAGGTTTTGTAACGAGTGGAAGCGATTCATTGAAAATCCAGAGCTTCTGATATTGCATATGAGATGA

Protein sequence

MCFVCRGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLSMAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDGSVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR

Homology

[Close]

BLAST of Cucsat.G1287.T4 vs. ExPASy Swiss-Prot
Match: Q9M7Z1 (Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BCE2 PE=1 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 1.6e-94
Identity = 166/226 (73.45%), Postives = 197/226 (87.17%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RGF RAMVK+M +A  VPHFH+VEEINCD+L+ELK  F+ N T+  +KHT LP LIKSLS
Sbjct: 258 RGFSRAMVKTMTMATSVPHFHFVEEINCDSLVELKQFFKENNTDSTIKHTFLPTLIKSLS 317

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           MA++KYP +NSCFN +S E+ LKGSHNIG+AMAT HGLVVPNIKNVQSLS+LEITKELSR
Sbjct: 318 MALTKYPFVNSCFNAESLEIILKGSHNIGVAMATEHGLVVPNIKNVQSLSLLEITKELSR 377

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQ LA  NKL+P D++GGTITLSNIGAIGGK+GSPLLNLPEV+IIA+GRI+KVP+ + +G
Sbjct: 378 LQHLAANNKLNPEDVTGGTITLSNIGAIGGKFGSPLLNLPEVAIIALGRIEKVPKFSKEG 437

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 232
           +VYP+SIM VNI ADHRVLDGATVARFC +WK ++E PELL+L MR
Sbjct: 438 TVYPASIMMVNIAADHRVLDGATVARFCCQWKEYVEKPELLMLQMR 483

[Close]

BLAST of Cucsat.G1287.T4 vs. ExPASy Swiss-Prot
Match: P11182 (Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial OS=Homo sapiens OX=9606 GN=DBT PE=1 SV=3)

HSP 1 Score: 218.8 bits (556), Expect = 6.5e-56
Identity = 103/226 (45.58%), Postives = 155/226 (68.58%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           +GFQ+AMVK+M+ A K+PHF Y +EI+   L++L+   +       +K + +P  +K+ S
Sbjct: 257 KGFQKAMVKTMSAALKIPHFGYCDEIDLTELVKLREELKPIAFARGIKLSFMPFFLKAAS 316

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           + + ++P+LN+  +E+   +T K SHNIGIAM T  GL+VPN+KNVQ  S+ +I  EL+R
Sbjct: 317 LGLLQFPILNASVDENCQNITYKASHNIGIAMDTEQGLIVPNVKNVQICSIFDIATELNR 376

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQ L    +LS  D++GGT TLSNIG+IGG +  P++  PEV+I A+G IK +P+    G
Sbjct: 377 LQKLGSVGQLSTTDLTGGTFTLSNIGSIGGTFAKPVIMPPEVAIGALGSIKAIPRFNQKG 436

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 232
            VY + IM V+  ADHRV+DGAT++RF N WK ++ENP  ++L ++
Sbjct: 437 EVYKAQIMNVSWSADHRVIDGATMSRFSNLWKSYLENPAFMLLDLK 482

[Close]

BLAST of Cucsat.G1287.T4 vs. ExPASy Swiss-Prot
Match: P53395 (Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial OS=Mus musculus OX=10090 GN=Dbt PE=1 SV=2)

HSP 1 Score: 218.0 bits (554), Expect = 1.1e-55
Identity = 105/225 (46.67%), Postives = 153/225 (68.00%), Query Frame = 0


Query: 7   GFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLSM 66
           GFQ+AMVK+M+ A K+PHF Y +EI+   L++L+   +       +K + +P  +K+ S+
Sbjct: 258 GFQKAMVKTMSAALKIPHFGYCDEIDLTQLVKLREELKPVALARGIKLSFMPFFLKAASL 317

Query: 67  AMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRL 126
            + ++P+LN+  +E+   +T K SHNIGIAM T  GL+VPN+KNVQ  SV EI  EL+RL
Sbjct: 318 GLLQFPILNASVDENCQNITYKASHNIGIAMDTELGLIVPNVKNVQVRSVFEIAMELNRL 377

Query: 127 QLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDGS 186
           Q L    +L   D++GGT TLSNIG+IGG Y  P++  PEV+I A+G IK +P+    G 
Sbjct: 378 QKLGSSGQLGTTDLTGGTFTLSNIGSIGGTYAKPVILPPEVAIGALGAIKALPRFDQKGD 437

Query: 187 VYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 232
           VY + IM V+  ADHRV+DGAT++RF N WK ++ENP  ++L ++
Sbjct: 438 VYKAQIMNVSWSADHRVIDGATMSRFSNLWKSYLENPAFMLLDLK 482

[Close]

BLAST of Cucsat.G1287.T4 vs. ExPASy Swiss-Prot
Match: P11181 (Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial OS=Bos taurus OX=9913 GN=DBT PE=1 SV=2)

HSP 1 Score: 215.7 bits (548), Expect = 5.5e-55
Identity = 102/226 (45.13%), Postives = 153/226 (67.70%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           +GF +AMVK+M+ A K+PHF Y +E++   L++L+   +       +K + +P  +K+ S
Sbjct: 257 KGFHKAMVKTMSAALKIPHFGYCDEVDLTELVKLREELKPIAFARGIKLSFMPFFLKAAS 316

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           + + ++P+LN+  +E+   +T K SHNIGIAM T  GL+VPN+KNVQ  S+ EI  EL+R
Sbjct: 317 LGLLQFPILNASVDENCQNITYKASHNIGIAMDTEQGLIVPNVKNVQIRSIFEIATELNR 376

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQ L    +LS  D+ GGT TLSNIG+IGG Y  P++  PEV+I A+G IK +P+  + G
Sbjct: 377 LQKLGSAGQLSTNDLIGGTFTLSNIGSIGGTYAKPVILPPEVAIGALGTIKALPRFNEKG 436

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 232
            V  + IM V+  ADHR++DGATV+RF N WK ++ENP  ++L ++
Sbjct: 437 EVCKAQIMNVSWSADHRIIDGATVSRFSNLWKSYLENPAFMLLDLK 482

[Close]

BLAST of Cucsat.G1287.T4 vs. ExPASy Swiss-Prot
Match: Q23571 (Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=dbt-1 PE=2 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 7.5e-52
Identity = 104/226 (46.02%), Postives = 152/226 (67.26%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RG+ RAMVK+M  A K+PHF Y +EIN D+L++ +A  +    E ++K + +P  IK+ S
Sbjct: 223 RGYTRAMVKTMTEALKIPHFGYNDEINVDSLVKYRAELKEFAKERHIKLSYMPFFIKAAS 282

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           +A+ +YP LNS  +E    V  K SHNI +AM TP GLVVPNIKN +  S+ EI +EL+R
Sbjct: 283 LALLEYPSLNSTTDEKMENVIHKASHNICLAMDTPGGLVVPNIKNCEQRSIFEIAQELNR 342

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           L     + ++   D+  GT +LSNIG IGG Y SP++  P+V+I AIG+I+K+P+     
Sbjct: 343 LLEAGKKQQIKREDLIDGTFSLSNIGNIGGTYASPVVFPPQVAIGAIGKIEKLPRFDKHD 402

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 232
           +V P +IM V+  ADHRV+DGAT+ARF N WK ++E+P  ++  ++
Sbjct: 403 NVIPVNIMKVSWCADHRVVDGATMARFSNRWKFYLEHPSAMLAQLK 448

[Close]

BLAST of Cucsat.G1287.T4 vs. NCBI nr
Match: XP_031739423.1 (lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 446 bits (1148), Expect = 5.75e-155
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS
Sbjct: 195 RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 254

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR
Sbjct: 255 MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 314

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG
Sbjct: 315 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 374

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 231
           SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR
Sbjct: 375 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 420

[Close]

BLAST of Cucsat.G1287.T4 vs. NCBI nr
Match: XP_031739422.1 (lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial isoform X1 [Cucumis sativus])

HSP 1 Score: 446 bits (1148), Expect = 7.83e-155
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS
Sbjct: 204 RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 263

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR
Sbjct: 264 MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 323

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG
Sbjct: 324 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 383

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 231
           SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR
Sbjct: 384 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 429

[Close]

BLAST of Cucsat.G1287.T4 vs. NCBI nr
Match: XP_004134223.1 (lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial isoform X3 [Cucumis sativus] >KGN57128.1 hypothetical protein Csa_010383 [Cucumis sativus])

HSP 1 Score: 446 bits (1148), Expect = 1.49e-153
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS
Sbjct: 292 RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 351

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR
Sbjct: 352 MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 411

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG
Sbjct: 412 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 471

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 231
           SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR
Sbjct: 472 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 517

[Close]

BLAST of Cucsat.G1287.T4 vs. NCBI nr
Match: XP_008438901.1 (PREDICTED: lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial [Cucumis melo])

HSP 1 Score: 441 bits (1133), Expect = 2.81e-151
Identity = 222/226 (98.23%), Postives = 224/226 (99.12%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS
Sbjct: 292 RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 351

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           MAMSKYPMLNSCFNED+FEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR
Sbjct: 352 MAMSKYPMLNSCFNEDAFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 411

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQLLAMENKLSPGDISGGTITLSNIGAIGGK+GSPLLNLPEVSIIAIGRIKKVPQI DDG
Sbjct: 412 LQLLAMENKLSPGDISGGTITLSNIGAIGGKFGSPLLNLPEVSIIAIGRIKKVPQITDDG 471

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 231
            VYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR
Sbjct: 472 GVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 517

[Close]

BLAST of Cucsat.G1287.T4 vs. NCBI nr
Match: KAA0049510.1 (lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex [Cucumis melo var. makuwa] >TYK16190.1 lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex [Cucumis melo var. makuwa])

HSP 1 Score: 441 bits (1133), Expect = 2.81e-151
Identity = 222/226 (98.23%), Postives = 224/226 (99.12%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS
Sbjct: 292 RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 351

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           MAMSKYPMLNSCFNED+FEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR
Sbjct: 352 MAMSKYPMLNSCFNEDAFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 411

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQLLAMENKLSPGDISGGTITLSNIGAIGGK+GSPLLNLPEVSIIAIGRIKKVPQI DDG
Sbjct: 412 LQLLAMENKLSPGDISGGTITLSNIGAIGGKFGSPLLNLPEVSIIAIGRIKKVPQITDDG 471

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 231
            VYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR
Sbjct: 472 GVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 517

[Close]

BLAST of Cucsat.G1287.T4 vs. ExPASy TrEMBL
Match: A0A0A0L5U6 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis sativus OX=3659 GN=Csa_3G164470 PE=3 SV=1)

HSP 1 Score: 446 bits (1148), Expect = 7.22e-154
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS
Sbjct: 292 RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 351

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR
Sbjct: 352 MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 411

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG
Sbjct: 412 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 471

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 231
           SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR
Sbjct: 472 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 517

[Close]

BLAST of Cucsat.G1287.T4 vs. ExPASy TrEMBL
Match: A0A5D3CXV6 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001180 PE=3 SV=1)

HSP 1 Score: 441 bits (1133), Expect = 1.36e-151
Identity = 222/226 (98.23%), Postives = 224/226 (99.12%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS
Sbjct: 292 RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 351

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           MAMSKYPMLNSCFNED+FEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR
Sbjct: 352 MAMSKYPMLNSCFNEDAFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 411

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQLLAMENKLSPGDISGGTITLSNIGAIGGK+GSPLLNLPEVSIIAIGRIKKVPQI DDG
Sbjct: 412 LQLLAMENKLSPGDISGGTITLSNIGAIGGKFGSPLLNLPEVSIIAIGRIKKVPQITDDG 471

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 231
            VYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR
Sbjct: 472 GVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 517

[Close]

BLAST of Cucsat.G1287.T4 vs. ExPASy TrEMBL
Match: A0A1S3AX39 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis melo OX=3656 GN=LOC103483861 PE=3 SV=1)

HSP 1 Score: 441 bits (1133), Expect = 1.36e-151
Identity = 222/226 (98.23%), Postives = 224/226 (99.12%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS
Sbjct: 292 RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 351

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           MAMSKYPMLNSCFNED+FEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR
Sbjct: 352 MAMSKYPMLNSCFNEDAFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 411

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQLLAMENKLSPGDISGGTITLSNIGAIGGK+GSPLLNLPEVSIIAIGRIKKVPQI DDG
Sbjct: 412 LQLLAMENKLSPGDISGGTITLSNIGAIGGKFGSPLLNLPEVSIIAIGRIKKVPQITDDG 471

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 231
            VYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR
Sbjct: 472 GVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 517

[Close]

BLAST of Cucsat.G1287.T4 vs. ExPASy TrEMBL
Match: A0A6J1C9A1 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Momordica charantia OX=3673 GN=LOC111009145 PE=3 SV=1)

HSP 1 Score: 418 bits (1075), Expect = 9.03e-143
Identity = 206/226 (91.15%), Postives = 220/226 (97.35%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RGFQRAMVKSM IAAKVPHFHYVEEINCD LLELKASFQGNT EP+VKHTSLPLLIKSLS
Sbjct: 294 RGFQRAMVKSMTIAAKVPHFHYVEEINCDGLLELKASFQGNTPEPDVKHTSLPLLIKSLS 353

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           MAM+KYPMLNSCFNE+S+EVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSV+EITKELSR
Sbjct: 354 MAMNKYPMLNSCFNEESYEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVMEITKELSR 413

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQ LA++NKL PGDISGGTITLSNIGAIGGK+GSPLLNLPEV+IIAIGRIKK+PQI+DDG
Sbjct: 414 LQQLALDNKLGPGDISGGTITLSNIGAIGGKFGSPLLNLPEVAIIAIGRIKKIPQISDDG 473

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 231
            VYP+SIMTVNIGADHRVLDGATVARFCNEWKRF+ENPELLIL+MR
Sbjct: 474 DVYPASIMTVNIGADHRVLDGATVARFCNEWKRFVENPELLILNMR 519

[Close]

BLAST of Cucsat.G1287.T4 vs. ExPASy TrEMBL
Match: A0A6J1G741 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucurbita moschata OX=3662 GN=LOC111451360 PE=3 SV=1)

HSP 1 Score: 402 bits (1034), Expect = 1.42e-136
Identity = 198/226 (87.61%), Postives = 217/226 (96.02%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RG+QRAMVKSM I+AKVPHFHYVEEINCD L+ELKA FQ NTTEP+VKHT LPLLIKSLS
Sbjct: 293 RGYQRAMVKSMTISAKVPHFHYVEEINCDGLVELKACFQENTTEPDVKHTFLPLLIKSLS 352

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           MAMSKYPM+NSCFNE+SFEVTLKGSHNIGIAMATP+GLVVPNIK+VQSLSVLEITKELSR
Sbjct: 353 MAMSKYPMVNSCFNEESFEVTLKGSHNIGIAMATPNGLVVPNIKSVQSLSVLEITKELSR 412

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQ LAM+NKLSP D+SGGTITLSNIGAIGGK+GSPLLNLPEV+IIA+GRIKKVPQI +DG
Sbjct: 413 LQRLAMDNKLSPVDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQITEDG 472

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 231
            VYP+SIMT+NIGADHRVLDGATV++FCNEWKRFIENP+LLILHMR
Sbjct: 473 DVYPASIMTINIGADHRVLDGATVSKFCNEWKRFIENPDLLILHMR 518

[Close]

BLAST of Cucsat.G1287.T4 vs. TAIR 10
Match: AT3G06850.1 (2-oxoacid dehydrogenases acyltransferase family protein )

HSP 1 Score: 347.1 bits (889), Expect = 1.1e-95
Identity = 166/226 (73.45%), Postives = 197/226 (87.17%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RGF RAMVK+M +A  VPHFH+VEEINCD+L+ELK  F+ N T+  +KHT LP LIKSLS
Sbjct: 258 RGFSRAMVKTMTMATSVPHFHFVEEINCDSLVELKQFFKENNTDSTIKHTFLPTLIKSLS 317

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           MA++KYP +NSCFN +S E+ LKGSHNIG+AMAT HGLVVPNIKNVQSLS+LEITKELSR
Sbjct: 318 MALTKYPFVNSCFNAESLEIILKGSHNIGVAMATEHGLVVPNIKNVQSLSLLEITKELSR 377

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQ LA  NKL+P D++GGTITLSNIGAIGGK+GSPLLNLPEV+IIA+GRI+KVP+ + +G
Sbjct: 378 LQHLAANNKLNPEDVTGGTITLSNIGAIGGKFGSPLLNLPEVAIIALGRIEKVPKFSKEG 437

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 232
           +VYP+SIM VNI ADHRVLDGATVARFC +WK ++E PELL+L MR
Sbjct: 438 TVYPASIMMVNIAADHRVLDGATVARFCCQWKEYVEKPELLMLQMR 483

[Close]

BLAST of Cucsat.G1287.T4 vs. TAIR 10
Match: AT3G06850.2 (2-oxoacid dehydrogenases acyltransferase family protein )

HSP 1 Score: 347.1 bits (889), Expect = 1.1e-95
Identity = 166/226 (73.45%), Postives = 197/226 (87.17%), Query Frame = 0


Query: 6   RGFQRAMVKSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLS 65
           RGF RAMVK+M +A  VPHFH+VEEINCD+L+ELK  F+ N T+  +KHT LP LIKSLS
Sbjct: 258 RGFSRAMVKTMTMATSVPHFHFVEEINCDSLVELKQFFKENNTDSTIKHTFLPTLIKSLS 317

Query: 66  MAMSKYPMLNSCFNEDSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSR 125
           MA++KYP +NSCFN +S E+ LKGSHNIG+AMAT HGLVVPNIKNVQSLS+LEITKELSR
Sbjct: 318 MALTKYPFVNSCFNAESLEIILKGSHNIGVAMATEHGLVVPNIKNVQSLSLLEITKELSR 377

Query: 126 LQLLAMENKLSPGDISGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKVPQIADDG 185
           LQ LA  NKL+P D++GGTITLSNIGAIGGK+GSPLLNLPEV+IIA+GRI+KVP+ + +G
Sbjct: 378 LQHLAANNKLNPEDVTGGTITLSNIGAIGGKFGSPLLNLPEVAIIALGRIEKVPKFSKEG 437

Query: 186 SVYPSSIMTVNIGADHRVLDGATVARFCNEWKRFIENPELLILHMR 232
           +VYP+SIM VNI ADHRVLDGATVARFC +WK ++E PELL+L MR
Sbjct: 438 TVYPASIMMVNIAADHRVLDGATVARFCCQWKEYVEKPELLMLQMR 483

[Close]

BLAST of Cucsat.G1287.T4 vs. TAIR 10
Match: AT3G52200.1 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 109.8 bits (273), Expect = 3.0e-24
Identity = 68/212 (32.08%), Postives = 111/212 (52.36%), Query Frame = 0


Query: 21  KVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLSMAMSKYPMLNSCFNE 80
           K+PH +   ++  D LL  +   Q N     VK +   ++IK++++A+      N+ ++ 
Sbjct: 429 KIPHLYLQSDVVLDPLLAFRKELQEN---HGVKVSVNDIVIKAVAVALRNVRQANAFWDA 488

Query: 81  DSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQLLAMENKLSPGDI 140
           +  ++ +  S +I IA+AT  GL+ P IKN    S+  I+ E+  L   A   KL+P + 
Sbjct: 489 EKGDIVMCDSVDISIAVATEKGLMTPIIKNADQKSISAISLEVKELAQKARSGKLAPHEF 548

Query: 141 SGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKV--PQIADDGSVYPSSI--MTVN 200
            GGT ++SN+G         ++N P+  I+A+GR  KV  P I  DG   PS +  M V 
Sbjct: 549 QGGTFSISNLGMYPVDNFCAIINPPQAGILAVGRGNKVVEPVIGLDGIEKPSVVTKMNVT 608

Query: 201 IGADHRVLDGATVARFCNEWKRFIENPELLIL 229
           + ADHR+ DG   A F +E +   E+   L+L
Sbjct: 609 LSADHRIFDGQVGASFMSELRSNFEDVRRLLL 637

[Close]

BLAST of Cucsat.G1287.T4 vs. TAIR 10
Match: AT3G52200.2 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 109.8 bits (273), Expect = 3.0e-24
Identity = 68/212 (32.08%), Postives = 111/212 (52.36%), Query Frame = 0


Query: 21  KVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLSMAMSKYPMLNSCFNE 80
           K+PH +   ++  D LL  +   Q N     VK +   ++IK++++A+      N+ ++ 
Sbjct: 505 KIPHLYLQSDVVLDPLLAFRKELQEN---HGVKVSVNDIVIKAVAVALRNVRQANAFWDA 564

Query: 81  DSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQLLAMENKLSPGDI 140
           +  ++ +  S +I IA+AT  GL+ P IKN    S+  I+ E+  L   A   KL+P + 
Sbjct: 565 EKGDIVMCDSVDISIAVATEKGLMTPIIKNADQKSISAISLEVKELAQKARSGKLAPHEF 624

Query: 141 SGGTITLSNIGAIGGKYGSPLLNLPEVSIIAIGRIKKV--PQIADDGSVYPSSI--MTVN 200
            GGT ++SN+G         ++N P+  I+A+GR  KV  P I  DG   PS +  M V 
Sbjct: 625 QGGTFSISNLGMYPVDNFCAIINPPQAGILAVGRGNKVVEPVIGLDGIEKPSVVTKMNVT 684

Query: 201 IGADHRVLDGATVARFCNEWKRFIENPELLIL 229
           + ADHR+ DG   A F +E +   E+   L+L
Sbjct: 685 LSADHRIFDGQVGASFMSELRSNFEDVRRLLL 713

[Close]

BLAST of Cucsat.G1287.T4 vs. TAIR 10
Match: AT1G54220.1 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 107.1 bits (266), Expect = 2.0e-23
Identity = 68/211 (32.23%), Postives = 113/211 (53.55%), Query Frame = 0


Query: 22  VPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSL-PLLIKSLSMAMSKYPMLNSCFNE 81
           +PH++   +   D L+ L++           K  S+  L++K+ ++A+ K P  NS + +
Sbjct: 332 IPHYYLTVDTCVDKLMALRSQLNSFKEASGGKRISVNDLVVKAAALALRKVPQCNSSWTD 391

Query: 82  DSFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQLLAMENKLSPGDI 141
           D   +    + NI +A+ T +GL VP +K+     +  I +E+  L   A EN L P D 
Sbjct: 392 D--YIRQFKNVNINVAVQTENGLYVPVVKDADRKGLSTIGEEVRLLAQKAKENSLKPEDY 451

Query: 142 SGGTITLSNIGA-IGGKYGSPLLNLPEVSIIAIGRIKK--VPQIADDGSVYPSSIMTVNI 201
            GGT T+SN+G   G K    ++N P+ +I+A+G  +K  VP    D   + +S M V +
Sbjct: 452 EGGTFTVSNLGGPFGIKQFCAVVNPPQAAILAVGSAEKRVVPGNGPDQFNF-ASYMPVTL 511

Query: 202 GADHRVLDGATVARFCNEWKRFIENPELLIL 229
             DHRV+DGA  A +   +K +IENP+ ++L
Sbjct: 512 SCDHRVVDGAIGAEWLKAFKGYIENPKSMLL 539

The following BLAST results are available for this feature:

BLAST of Cucsat.G1287.T4 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M7Z1	1.6e-94	73.45	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
P11182	6.5e-56	45.58	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
P53395	1.1e-55	46.67	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
P11181	5.5e-55	45.13	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
Q23571	7.5e-52	46.02	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]

BLAST of Cucsat.G1287.T4 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739423.1	5.75e-155	100.00	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
XP_031739422.1	7.83e-155	100.00	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
XP_004134223.1	1.49e-153	100.00	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
XP_008438901.1	2.81e-151	98.23	PREDICTED: lipoamide acyltransferase component of branched-chain alpha-keto acid...	[more]
KAA0049510.1	2.81e-151	98.23	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]

BLAST of Cucsat.G1287.T4 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5U6	7.22e-154	100.00	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O...	[more]
A0A5D3CXV6	1.36e-151	98.23	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O...	[more]
A0A1S3AX39	1.36e-151	98.23	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O...	[more]
A0A6J1C9A1	9.03e-143	91.15	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O...	[more]
A0A6J1G741	1.42e-136	87.61	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O...	[more]

BLAST of Cucsat.G1287.T4 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06850.1	1.1e-95	73.45	2-oxoacid dehydrogenases acyltransferase family protein	[more]
AT3G06850.2	1.1e-95	73.45	2-oxoacid dehydrogenases acyltransferase family protein	[more]
AT3G52200.1	3.0e-24	32.08	Dihydrolipoamide acetyltransferase, long form protein	[more]
AT3G52200.2	3.0e-24	32.08	Dihydrolipoamide acetyltransferase, long form protein	[more]
AT1G54220.1	2.0e-23	32.23	Dihydrolipoamide acetyltransferase, long form protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001078	2-oxoacid dehydrogenase acyltransferase, catalytic domain	PFAM	PF00198	2-oxoacid_dh	coord: 6..227 e-value: 6.9E-73 score: 244.9
IPR023213	Chloramphenicol acetyltransferase-like domain superfamily	GENE3D	3.30.559.10		coord: 4..230 e-value: 8.7E-78 score: 263.2
None	No IPR available	PANTHER	PTHR43178	DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX	coord: 5..231
None	No IPR available	PANTHER	PTHR43178:SF5	LIPOAMIDE ACYLTRANSFERASE COMPONENT OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX, MITOCHONDRIAL	coord: 5..231
None	No IPR available	SUPERFAMILY	52777	CoA-dependent acyltransferases	coord: 6..228

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G1287	Cucsat.G1287	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G1287.T4.E1	Cucsat.G1287.T4.E1	exon
Cucsat.G1287.T4.E2	Cucsat.G1287.T4.E2	exon
Cucsat.G1287.T4.E3	Cucsat.G1287.T4.E3	exon
Cucsat.G1287.T4.E4	Cucsat.G1287.T4.E4	exon
Cucsat.G1287.T4.E5	Cucsat.G1287.T4.E5	exon
Cucsat.G1287.T4.E6	Cucsat.G1287.T4.E6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G1287.T4.C1	Cucsat.G1287.T4.C1	CDS
Cucsat.G1287.T4.C2	Cucsat.G1287.T4.C2	CDS
Cucsat.G1287.T4.C3	Cucsat.G1287.T4.C3	CDS
Cucsat.G1287.T4.C4	Cucsat.G1287.T4.C4	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G1287.T4	Cucsat.G1287.T4-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0016746	acyltransferase activity
molecular_function	GO:0016407	acetyltransferase activity
molecular_function	GO:0031405	lipoic acid binding