CmoCh02G015390.1 (mRNA) Cucurbita moschata (Rifu) v1

BLAST of CmoCh02G015390.1 vs. ExPASy Swiss-Prot
Match: A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 3.9e-280
Identity = 494/756 (65.34%), Postives = 583/756 (77.12%), Query Frame = 0

Query: 4   FPSLFLLLLLNFHYVGALVTELPLINLQT--------YIVHVKKPE-----TTDDLEIWH 63
           F  + L  LL FH   A  +ELP    ++        YI+HV  PE      ++DLE W+
Sbjct: 4   FLFIALTFLLTFHVHNAQGSELPTTTTESTETSSSKIYIIHVTGPEGKMLTESEDLESWY 63

Query: 64  RSFLP-------SSSSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTT 123
            SFLP           ++YSY+NV+ GFAA L++E++ A+E+ +GF+SA  +R+L   TT
Sbjct: 64  HSFLPPTLMSSEEQPRVIYSYKNVLRGFAASLTQEELSAVEKKNGFISAHPQRVLHRQTT 123

Query: 124 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNF 183
           HTP FLGL +  G WK+SNFGKGVIIGVLD GI P HPSF DVG+PPPPPKWKGRC+ N 
Sbjct: 124 HTPKFLGLQQDTGVWKESNFGKGVIIGVLDSGITPGHPSFSDVGIPPPPPKWKGRCDLNV 183

Query: 184 SACNNKLIGARSFNLATKALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKG 243
           +ACNNKLIGAR+FNLA +A+ G+    ++PIDEDGHGTHTASTAAGAFV  AE LGNAKG
Sbjct: 184 TACNNKLIGARAFNLAAEAMNGKKA--EAPIDEDGHGTHTASTAAGAFVNYAEVLGNAKG 243

Query: 244 TAVGMAPLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLG-SPSVPFFQDLVA 303
           TA GMAP AHLAIYKVCFGEDCP++DILAALDAA+EDGVDV+S+SLG S   PFF D  A
Sbjct: 244 TAAGMAPHAHLAIYKVCFGEDCPESDILAALDAAVEDGVDVISISLGLSEPPPFFNDSTA 303

Query: 304 IGAFAAIQKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDG 363
           IGAFAA+QKGIFVSC+A NSGPF +++ N APWILTV ASTIDRRI A AKLGNG+EFDG
Sbjct: 304 IGAFAAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTVGASTIDRRIVATAKLGNGQEFDG 363

Query: 364 ESLFQPSDFPPTLLPLVYAGEK-NQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGT 423
           ES+FQPS F PTLLPL YAG+   + +A C  GSL D   +GKVV+CERGGGIARIAKG 
Sbjct: 364 ESVFQPSSFTPTLLPLAYAGKNGKEESAFCANGSLDDSAFRGKVVLCERGGGIARIAKGE 423

Query: 424 EVKNAGGAAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKG 483
           EVK AGGAAMIL+N + + FS  AD H LPA+HVS+ A ++IKAYINST  PTATILFKG
Sbjct: 424 EVKRAGGAAMILMNDETNAFSLSADVHALPATHVSYAAGIEIKAYINSTATPTATILFKG 483

Query: 484 TVIGDDNFSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNII 543
           TVIG ++ +PA+ASFSSRGP++ SPGILKPDI GPGV+ILAAWPFPL  + ++K TFNI 
Sbjct: 484 TVIG-NSLAPAVASFSSRGPNLPSPGILKPDIIGPGVNILAAWPFPLSNSTDSKLTFNIE 543

Query: 544 SGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFAT 603
           SGTSMSCPHLSGIAAL+KSSHP WSPAAIKSAIMT+AD  NL  + IVDE LQP DLFAT
Sbjct: 544 SGTSMSCPHLSGIAALLKSSHPHWSPAAIKSAIMTSADTINLGNKLIVDETLQPTDLFAT 603

Query: 604 GAGHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLN 663
           G+GHVNPS+A DPGLVYDIQPDDYIPYLCGLGY   EV  IA + I C A  SIPEG+LN
Sbjct: 604 GSGHVNPSRANDPGLVYDIQPDDYIPYLCGLGYSETEVGIIAHRKIKCSA--SIPEGELN 663

Query: 664 YPSFTVVLGPPQTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSV 723
           YPSF+V LG  +TFTRTVTNVG     Y  +V AP  + V ++P K+ FS++NQK T+SV
Sbjct: 664 YPSFSVELGSSKTFTRTVTNVGEAHSSYDLIVAAPQGVDVKVQPYKLNFSEVNQKETYSV 723

Query: 724 TFKRIGSISPSTEFGKGYLKWVSDKHVVRSPISFKF 738
           TF R G  + + E+ +G+LKWVS KH VRSPIS KF
Sbjct: 724 TFSRTGLGNKTQEYAQGFLKWVSTKHTVRSPISVKF 754

BLAST of CmoCh02G015390.1 vs. ExPASy Swiss-Prot
Match: A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 5.2e-248
Identity = 449/725 (61.93%), Postives = 549/725 (75.72%), Query Frame = 0

Query: 29  NLQTYIVHVKKPE------TTDDLEIWHRSFLPSSSS---LLYSYRNVMSGFAARLSEEQ 88
           NL TYIVHVKK E      +T++L  WH SFLP +S+   +++SYRNV SGFA RL+ E+
Sbjct: 37  NLTTYIVHVKKLEIEGPLQSTEELHTWHHSFLPETSNKDRMVFSYRNVASGFAVRLTPEE 96

Query: 89  VKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPS 148
             A++E +  +S R ER L LHTTHTP FLGL +  G W DSN GKGVIIGV+D GI P 
Sbjct: 97  ANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDTGIYPF 156

Query: 149 HPSFDDVGMPPPPPKWKGRCEF-NFSACNNKLIGARSFNLATKALKGETMMDDSPIDEDG 208
           H SF+D GMPPPP KWKG CEF   S CNNKLIGAR  NL   A++      + P ++  
Sbjct: 157 HLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGAR--NLVKSAIQ------EPPYEDFF 216

Query: 209 HGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVCFG---EDCPDTDILAALD 268
           HGTHTA+ AAG FV+GA   GNA+GTA GMAP AHLAIYKVC     ++CP++ ILAA+D
Sbjct: 217 HGTHTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAILAAMD 276

Query: 269 AAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAANSGPFKATLSNEAPW 328
            AIEDGVDVLSLSLG  S+PFF+D +AIGAFAA QKGIFVSCSAANSGP  ++LSNEAPW
Sbjct: 277 IAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSNEAPW 336

Query: 329 ILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY-AGEKNQTAALCGEG 388
           ILTV ASTIDR+I A+AKLGNG E++GE+LFQP DF   LLPLVY A EKN ++ALC  G
Sbjct: 337 ILTVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSALCAPG 396

Query: 389 SLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDGFSTEADAHVLPASH 448
           SL++I+VKGKVVVC+ GGGI  IAKG EV +AGG+AMIL N +  GF+T A+AHVLPA H
Sbjct: 397 SLRNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHVLPAVH 456

Query: 449 VSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRGPSVASPGILKPDIT 508
           VS+ A+L IKAYINST  PTAT+LF+GT+IG D+ +P++A+FSSRGPS  SPGILKPDI 
Sbjct: 457 VSYAASLAIKAYINSTYTPTATVLFQGTIIG-DSLAPSVAAFSSRGPSQQSPGILKPDII 516

Query: 509 GPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAI 568
           GPGV+ILAAW   +D   N    F+IISGTSMSCPHLSGIAAL+KS+HPDWSPAAIKSAI
Sbjct: 517 GPGVNILAAWAVSVD---NKIPAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAIKSAI 576

Query: 569 MTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLGY 628
           MTTA+  NL G PI+D+ LQPAD+FATGAGHVNP +A DPGLVYDIQP+DY+PYLCGLGY
Sbjct: 577 MTTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLCGLGY 636

Query: 629 KSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLG-PPQTFTRTVTNVGCGREVYTAVV 688
              EV  I ++ + C    SI + +LNYPSF+++LG   Q +TRT+TNVG     YT  +
Sbjct: 637 SDREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTYTVKI 696

Query: 689 EAPPSISVTIRPSKIFFSKINQKVTFSVTF-KRIGSISPSTEFGKGYLKWVSDKHVVRSP 738
           + P ++ +++ PS+I F+++NQKV + V F  +I     +  F +G + WVSDKHVVR+P
Sbjct: 697 DVPLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHVVRTP 749

BLAST of CmoCh02G015390.1 vs. ExPASy Swiss-Prot
Match: A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 6.8e-248
Identity = 445/742 (59.97%), Postives = 552/742 (74.39%), Query Frame = 0

Query: 9   LLLLLNFHYVGALVTELPLINLQTYIVHVKKPE-----TTDDLEIWHRSFLPSS------ 68
           +  LL+FH    L T      LQTYIVHV KP+      + DLE ++ SFLP++      
Sbjct: 9   IAFLLSFH----LTTAQRSTGLQTYIVHVDKPDAQVLANSADLESYYNSFLPATVSGSEV 68

Query: 69  -SSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFG 128
            S +++SY +V +GFAA+LS E+VK ME+  GFVSA+ E++L LHTTHTP+FLGL +  G
Sbjct: 69  PSRIIHSYHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLGLYQNMG 128

Query: 129 FWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSA-CNNKLIGARS 188
           FW++SN+GKGVIIG+LD GI P HPSF DV MP PP KWKG+CEF  +A CN K+IGAR+
Sbjct: 129 FWQESNYGKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFTGNATCNKKIIGARN 188

Query: 189 FNLATKALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLA 248
           F      + G  +    P DE+GHGTHTASTAAG FV  A   GNA GTAVGMAPLAH+A
Sbjct: 189 F------ISGSGV---PPTDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGMAPLAHIA 248

Query: 249 IYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFV 308
           +YKVC  + C D DILAALDAAI+DGVDVLSLSLG  S PF+ D +AIGAFAAI+KGIFV
Sbjct: 249 MYKVCSEDGCSDADILAALDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAAIRKGIFV 308

Query: 309 SCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTL 368
           S SA N GP  +TLSNEAPWILTV AST DR+I A A LGNG+++DGES FQP+DFP TL
Sbjct: 309 SASAGNDGPLNSTLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESAFQPADFPHTL 368

Query: 369 LPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLN 428
           LPLVY G  ++ AA C  GSL   DVKGKVVVC+RGG +AR+ K   VK+AGGAAMIL N
Sbjct: 369 LPLVYPGTSDEEAAFCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAGGAAMILAN 428

Query: 429 QQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIAS 488
            + DG  T ADAHVLPA+HV + A   IK+YINST+ PTA ILFKGT+IG  + SP+++S
Sbjct: 429 LEIDGEGTFADAHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTIIGFKS-SPSVSS 488

Query: 489 FSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIA 548
           FSSRGP++ASPGI+KPDI GPGV+ILAAWP  ++    T  TFNIISGTSMSCPHLSGI 
Sbjct: 489 FSSRGPNLASPGIVKPDIIGPGVNILAAWPVSVENKTGTDLTFNIISGTSMSCPHLSGIV 548

Query: 549 ALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPG 608
           AL+KS+HPDWSPAAIKSAIMT+AD +NLEGQPI+DE   PAD+FATGAGHVNPSKA+DPG
Sbjct: 549 ALLKSAHPDWSPAAIKSAIMTSADQSNLEGQPILDERNLPADIFATGAGHVNPSKASDPG 608

Query: 609 LVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGP-PQT 668
           L+YDIQ +DYI YLCGLGY+  ++  I ++ + C  + SI E +LNYPSF+++LGP  Q 
Sbjct: 609 LIYDIQLEDYIQYLCGLGYREKDIGLIVQETVKC--ESSISEAELNYPSFSIILGPKTQN 668

Query: 669 FTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTE 728
           +TRTVTNVG     YT  +     + + + P+ + F+K+ Q+ T++V+F + G    +  
Sbjct: 669 YTRTVTNVGDASSTYTVNIAKTQGVDIVVEPATLVFTKMYQQATYTVSFTQSGDF--TDR 728

Query: 729 FGKGYLKWVSDKHVVRSPISFK 737
           F +G + W S+++VVRSPIS K
Sbjct: 729 FVQGAISWSSNEYVVRSPISVK 732

BLAST of CmoCh02G015390.1 vs. ExPASy Swiss-Prot
Match: A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 6.3e-238
Identity = 430/720 (59.72%), Postives = 529/720 (73.47%), Query Frame = 0

Query: 29  NLQTYIVHVKKPET--TDDLEIWHRSFLPSSSS-----LLYSYRNVMSGFAARLSEEQVK 88
           NL TYIVHV+KP+   +DDL  ++ S LP S+      ++++YRNV++GFA +L+ E+ K
Sbjct: 41  NLSTYIVHVRKPQVIQSDDLHTFYYSLLPESTKTTNQRIVFTYRNVVNGFAVKLTPEEAK 100

Query: 89  AMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPSHP 148
           A+++ +  VSAR E+IL LHTTHTP FLGL +  G WK SN GKGVIIG+LD GI+P HP
Sbjct: 101 ALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKGVIIGILDTGISPFHP 160

Query: 149 SFDDVGMPPPPPKWKGRCEFNFS-ACNNKLIGARSFNLATKALKGETMMDDSPIDEDGHG 208
           SF D GMP PP KW G CEF     CNNK+IGAR+F + TK L         P D+ GHG
Sbjct: 161 SFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNF-VKTKNL-------TLPFDDVGHG 220

Query: 209 THTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVCFGEDCPDTDILAALDAAIED 268
           THTASTAAG  V+GA   GNA GTAVGMAP AH+A+YKVC    C ++ ILA +D A++D
Sbjct: 221 THTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAILAGMDTAVDD 280

Query: 269 GVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAANSGPFKATLSNEAPWILTVA 328
           GVDVLSLSLG PS PFF+D +A+GAF AIQKGIFVSCSAANSGP  ++LSNEAPWILTV 
Sbjct: 281 GVDVLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLSNEAPWILTVG 340

Query: 329 ASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVYAGE--KNQTAALCGEGSLKD 388
           AS+IDR I A AKLGNG+E+ G+S+FQP DF P+LLPLVYAG    N  +  C   SL  
Sbjct: 341 ASSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGANGNNNFSVFCAPESLNR 400

Query: 389 IDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDGFSTEADAHVLPASHVSHK 448
            DV+GKVV+CE GG + R+ KG  VK+AGGAAMIL+N   + F+  AD HVLPA H+S++
Sbjct: 401 SDVEGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADVHVLPAVHISYE 460

Query: 449 AALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRGPSVASPGILKPDITGPGV 508
           A L +K YINST+ PTATILF+GTVIG +  +P + SFSSRGPS ASPGILKPDI GPG+
Sbjct: 461 AGLALKEYINSTSTPTATILFEGTVIG-NLLAPQVTSFSSRGPSKASPGILKPDIIGPGL 520

Query: 509 SILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTA 568
           +ILAAWP  LD   +T   FNIISGTSMSCPHLSGIAAL+K+SHPDWSPAAIKSAIMTTA
Sbjct: 521 NILAAWPVSLD--NSTTPPFNIISGTSMSCPHLSGIAALLKNSHPDWSPAAIKSAIMTTA 580

Query: 569 DITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKSNE 628
              NL G PI+D+ L PAD+FATGAGHVNP KA DPGLVYDI+P+DYIPYLCGL Y   E
Sbjct: 581 SQVNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPNDYIPYLCGLNYTDRE 640

Query: 629 VATIARKPINCLAKPSIPEGDLNYPSFTVVLG-PPQTFTRTVTNVGCGREVYTAVVEAPP 688
           V  I ++ + C     I E +LNYPSF+++LG   Q +TRTV NVG     YTA +  P 
Sbjct: 641 VGVILQQRVRCSEVNHIAEAELNYPSFSILLGNTTQLYTRTVANVGPANSTYTAEIGVPV 700

Query: 689 SISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYLKWVSDKHVVRSPISFKF 738
            + +++ P+++ F+++ QK+T+SV+F        +  F +G LKWVS K+ VRSPISF F
Sbjct: 701 GVGMSLSPAQLTFTEVGQKLTYSVSFIPFSEDRDNHTFAQGSLKWVSGKYSVRSPISFIF 749

BLAST of CmoCh02G015390.1 vs. ExPASy Swiss-Prot
Match: G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 2.9e-235
Identity = 434/728 (59.62%), Postives = 527/728 (72.39%), Query Frame = 0

Query: 28  INLQTYIVHVKKPET-----TDDLEIWHRSFLPSS----SSLLYSYRNVMSGFAARLSEE 87
           INL TYIVHVKK E      ++DL  W+ SFLP +      +++SYR V SGFA +L+ E
Sbjct: 33  INLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQTFPHKERMVFSYRKVASGFAVKLTPE 92

Query: 88  QVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAP 147
           + K+++E    VSAR ER L+LHTTHTP FLGL +  G W D N GKGVIIG++D GI P
Sbjct: 93  EAKSLQEKGEIVSARPERTLELHTTHTPTFLGLKQGQGLWSDDNLGKGVIIGIIDTGIFP 152

Query: 148 SHPSFDDVGMPPPPPKWKGRCEF-NFSACNNKLIGARSFNLATKALKGETMMDDSPIDED 207
            HPSF+D GMPPPP KWKG CEF     CNNKLIGAR  NL   A++      + P +  
Sbjct: 153 LHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLIGAR--NLVKSAIQ------EPPFENF 212

Query: 208 GHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVCFGE-DCPDTDILAALDA 267
            HGTHTA+ AAG F++ A   GNAKG A GMAP AHLAIYKVC  +  C ++ ILAA+D 
Sbjct: 213 FHGTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNAHLAIYKVCNDKIGCTESAILAAMDI 272

Query: 268 AIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAANSGPFKATLSNEAPWI 327
           AIEDGVDVLSLSLG  S+PFF+D +AIGAFAA Q G+FVSCSAANSGP  +TLSNEAPWI
Sbjct: 273 AIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQNGVFVSCSAANSGPGYSTLSNEAPWI 332

Query: 328 LTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVYA-----GEKNQTAALC 387
           LTV ASTIDR+I A+AKLGNGEE++GE+LFQP DF   LLPLVY      G + Q  +LC
Sbjct: 333 LTVGASTIDRKIVASAKLGNGEEYEGETLFQPKDFSQQLLPLVYPGSFGYGNQTQNQSLC 392

Query: 388 GEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDGFSTEADAHVLP 447
             GSLK+ID+ GKVV+C+  G ++ I KG EV N+GG AMIL N +  GFST A AHVLP
Sbjct: 393 LPGSLKNIDLSGKVVLCD-VGNVSSIVKGQEVLNSGGIAMILANSEALGFSTFAIAHVLP 452

Query: 448 ASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRGPSVASPGILKP 507
           A  VS+ A L IK+YI ST  PTAT++FKGT+IG D+ +P++  FSSRGPS  SPGILKP
Sbjct: 453 AVEVSYAAGLTIKSYIKSTYNPTATLIFKGTIIG-DSLAPSVVYFSSRGPSQESPGILKP 512

Query: 508 DITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKSSHPDWSPAAIK 567
           DI GPGV+ILAAW   +D   N    F+I+SGTSMSCPHLSGIAALIKSSHPDWSPAAIK
Sbjct: 513 DIIGPGVNILAAWAVSVD---NKIPAFDIVSGTSMSCPHLSGIAALIKSSHPDWSPAAIK 572

Query: 568 SAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDIQPDDYIPYLCG 627
           SAIMTTA+  NL G PI+D+ L PAD+FATGAGHVNP KA DPGLVYDI+P+DY+PYLCG
Sbjct: 573 SAIMTTANTLNLGGIPILDQRLFPADIFATGAGHVNPVKANDPGLVYDIEPEDYVPYLCG 632

Query: 628 LGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLG-PPQTFTRTVTNVGCGREVYT 687
           LGY   E+  I +  + C    SIPE  LNYPSF+++LG   Q +TRT+TNVG     Y 
Sbjct: 633 LGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYPSFSILLGSDSQYYTRTLTNVGFANSTYK 692

Query: 688 AVVEAPPSISVTIRPSKIFFSKINQKVTFSVTF-KRIGSISPSTEFGKGYLKWVSDKHVV 738
             +E P ++ +++ PS+I F+++N+KV+FSV F  +I     +  FG+G L WVSD+H V
Sbjct: 693 VELEVPLALGMSVNPSEITFTEVNEKVSFSVEFIPQIKENRRNHTFGQGSLTWVSDRHAV 747

BLAST of CmoCh02G015390.1 vs. ExPASy TrEMBL
Match: A0A6J1H242 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111459745 PE=3 SV=1)

HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 738/738 (100.00%), Postives = 738/738 (100.00%), Query Frame = 0

Query: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60
           MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL
Sbjct: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60

Query: 61  LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
           LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD
Sbjct: 61  LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120

Query: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
           SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT
Sbjct: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180

Query: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
           KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC
Sbjct: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240

Query: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
           FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA
Sbjct: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300

Query: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
           NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY
Sbjct: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360

Query: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
           AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG
Sbjct: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420

Query: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
           FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG
Sbjct: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480

Query: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
           PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS
Sbjct: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540

Query: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
           SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI
Sbjct: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600

Query: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
           QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT
Sbjct: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660

Query: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
           NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL
Sbjct: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720

Query: 721 KWVSDKHVVRSPISFKFA 739
           KWVSDKHVVRSPISFKFA
Sbjct: 721 KWVSDKHVVRSPISFKFA 738

BLAST of CmoCh02G015390.1 vs. ExPASy TrEMBL
Match: A0A5A7TJ55 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003250 PE=3 SV=1)

HSP 1 Score: 1238.4 bits (3203), Expect = 0.0e+00
Identity = 623/745 (83.62%), Postives = 663/745 (88.99%), Query Frame = 0

Query: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSS--- 60
           MV  PSLFLLLLLNFH   A VTELPL NL TYIVHVKKPE  DDLEIWHRSFLP+S   
Sbjct: 1   MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60

Query: 61  ----SSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNR 120
                +LLYSYRNVMSGF+ARL+EE VKAMEE DGFVSARRE I+ LHTTHTPDFLGLNR
Sbjct: 61  EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120

Query: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
           QFGFWKDSNFGKGVIIGVLDGGI P+HPSFDD GMP PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180

Query: 181 RSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
           RS NLA++ALKG+ T +DDSPIDEDGHGTHTASTAAG FV GAEALGNA GTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240

Query: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
           HLAIYKVCFGEDC D DILA LDAA+EDGVDVLS+SLG PSVPFF D+ AIG+FAAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300

Query: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
           IFVSCSAANSGPF ATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360

Query: 361 PTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMI 420
            TLLPLV+ GEKN+T ALC EGSLK+IDVKGKVVVCERGGGIARIAKG EVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420

Query: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
           LLN + DGF+TE DAHVLPASHVSH AALKIKAYINSTTYPTATILFKGT IGDD FSPA
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDD-FSPA 480

Query: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLS 540
           IASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFNIISGTSMSCPHLS
Sbjct: 481 IASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLS 540

Query: 541 GIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAA 600
           GIAALIKS+HPDWSPAAIKS+IMTTA+ITNLEG PI+DE LQPADLFA GAGHVNPSKA 
Sbjct: 541 GIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAI 600

Query: 601 DPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPP 660
           DPGLVYDIQPDDYIPYLCGLGY +N+V+ IA KPI+CL   SIPEG+LNYPSF V LGP 
Sbjct: 601 DPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPV 660

Query: 661 QTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPS 720
           QTF+RTVT+VG GR VY  V+EAP  +SVT+RP K+ FS +NQK T+SVTFKRIGSISPS
Sbjct: 661 QTFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPS 720

Query: 721 TEFGKGYLKWVSDKHVVRSPISFKF 738
            EF +GYLKWVS KHVVRSPIS KF
Sbjct: 721 IEFAEGYLKWVSAKHVVRSPISVKF 744

BLAST of CmoCh02G015390.1 vs. ExPASy TrEMBL
Match: A0A1S3ATZ7 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482686 PE=3 SV=1)

HSP 1 Score: 1233.0 bits (3189), Expect = 0.0e+00
Identity = 621/745 (83.36%), Postives = 661/745 (88.72%), Query Frame = 0

Query: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSS--- 60
           MV  PSLFLLLLLNFH   A VTELPL NL TYIVHVKKPE  DDLEIWHRSFLP+S   
Sbjct: 1   MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60

Query: 61  ----SSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNR 120
                +LLYSYRNVMSGF+ARL+EE VKAMEE DGFVSARRE I+ LHTTHTPDFLGLNR
Sbjct: 61  EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120

Query: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
           QFGFWKDSNFGKGVIIGVLDGGI P+HPSFDD GM  PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFNFSACNNKLIGA 180

Query: 181 RSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
           RS NLA++ALKG+ T +DDSPIDEDGHGTHTASTAAG FV GAEALGNA GTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240

Query: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
           HLAIYKVCFGEDC D DILA LDAA+EDGVDVLS+SLG PSVPFF D+ AIG+FAAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300

Query: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
           IFVSCSAANSGPF ATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360

Query: 361 PTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMI 420
            TLLPLV+ GEKN+T ALC EGSLK+IDVKGKVVVCERGGGIARIAKG EVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420

Query: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
           LLN + DGF+TE DAHVLPASHVSH AALKIKAYINSTTYPTATILFKGT IGDD FSPA
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDD-FSPA 480

Query: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLS 540
           IASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFNIISGTSMSCPHLS
Sbjct: 481 IASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLS 540

Query: 541 GIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAA 600
           GIAALIKS+HPDWSPAAIKS+IMTTA+ITNLEG PI+DE LQPADLFA GAGHVNPSKA 
Sbjct: 541 GIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAI 600

Query: 601 DPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPP 660
           DPGLVYDIQPDDYIPYLCGLGY +N+V+ IA KPI+CL   SIPEG+LNYPSF V LGP 
Sbjct: 601 DPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPV 660

Query: 661 QTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPS 720
           QTF+RTVT+VG GR VY  V+EAP  +SVT+RP K+ FS +NQK T+SVTFKR GSISPS
Sbjct: 661 QTFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRSGSISPS 720

Query: 721 TEFGKGYLKWVSDKHVVRSPISFKF 738
            EF +GYLKWVS KHVVRSPIS KF
Sbjct: 721 IEFAEGYLKWVSAKHVVRSPISVKF 744

BLAST of CmoCh02G015390.1 vs. ExPASy TrEMBL
Match: A0A0A0KKE3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157240 PE=3 SV=1)

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 614/745 (82.42%), Postives = 659/745 (88.46%), Query Frame = 0

Query: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSS--- 60
           MV  PSLFLLLLLNFH   A VTELP  NL TYIVHVKKPE  DDLE WHRSFLP+S   
Sbjct: 1   MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60

Query: 61  ----SSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNR 120
                +LLYSYRNVMSGF+ARL+EE VKAMEE DGFVSARRE I+ LHTTH+P+FLGLNR
Sbjct: 61  SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120

Query: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
           QFGFWKDSNFGKGVIIGVLDGGI PSHPSF D GMP PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180

Query: 181 RSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
           RS NLA++ALKG+ T +DDSPIDEDGHGTHTASTAAG FV GAEALGNA GTAVGMAPLA
Sbjct: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240

Query: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
           HLAIYKVCFGE C + DILA LDAA+EDGVDVLS+SLG P VPFF D+ AIGAFAAIQKG
Sbjct: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300

Query: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
           IFVSCSAANSGPF ATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360

Query: 361 PTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMI 420
            T LPLV+ GEKN+T ALC EGSLK+IDVKGKVVVC+RGGGIARIAKG EVKNAGGAAMI
Sbjct: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420

Query: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
           LLN + DGF+TEADAHVLPASHVSH AALKIKAYINSTTYPTATI+FKGT IGDD FSPA
Sbjct: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDD-FSPA 480

Query: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLS 540
           IA+FSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFNI+SGTSMSCPHLS
Sbjct: 481 IAAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLS 540

Query: 541 GIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAA 600
           GIAALIKS+HPDWSPAAIKS+IMTTA+ITNLEG PIVD+ LQPADLFA GAGHVNPSKA 
Sbjct: 541 GIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAV 600

Query: 601 DPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPP 660
           DPGLVYDIQPDDYIPYLCGLGY +N+V+ IA KPI+CL   SIPEG+LNYPSF V LG  
Sbjct: 601 DPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQV 660

Query: 661 QTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPS 720
           QTF+RTVT VG GREVY  V+EAP  +SVT+RP K+ FS +NQK T+SVTFKRIGSISPS
Sbjct: 661 QTFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPS 720

Query: 721 TEFGKGYLKWVSDKHVVRSPISFKF 738
           TEF +GYLKWVS KH+VRSPIS KF
Sbjct: 721 TEFAEGYLKWVSAKHLVRSPISVKF 744

BLAST of CmoCh02G015390.1 vs. ExPASy TrEMBL
Match: A0A6J1E0E3 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111025997 PE=3 SV=1)

HSP 1 Score: 1213.7 bits (3139), Expect = 0.0e+00
Identity = 622/752 (82.71%), Postives = 667/752 (88.70%), Query Frame = 0

Query: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPE------TTDDLEIWHRSFL 60
           MV  PSLFLL+L +FH  GA  T     NLQTYIVHVKKPE        +DLE WHRSFL
Sbjct: 1   MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60

Query: 61  PSSS----SLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLG 120
           PSSS     LLYS+ NVMSGFAARL+EE VKAMEE DGF+SARRERIL+LHTTHTP+FLG
Sbjct: 61  PSSSEDHPKLLYSFXNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLG 120

Query: 121 LNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKL 180
           LNRQFGFWKDSNFGKG IIGVLDGGI P+HPSFDD GMPPPP KWKGRCEFNFSACNNKL
Sbjct: 121 LNRQFGFWKDSNFGKGXIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACNNKL 180

Query: 181 IGARSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMA 240
           IGARSFNLATKALKGE     DSPIDEDGHGTHTASTAAGAFV  AEALGNAKGTAVGMA
Sbjct: 181 IGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMA 240

Query: 241 PLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAI 300
           PLAHLAIYKVCFGEDCPD+DILAALDAA+EDGVDVLSLSLGS S PFF+D +AIGAFAA 
Sbjct: 241 PLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAAT 300

Query: 301 QKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPS 360
           QKGIFVSCSA+NSGP  ATLSNEAPWILTVAASTIDRRI A AKLGNGEEFDGESLFQPS
Sbjct: 301 QKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPS 360

Query: 361 DFPPTL-LPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGG 420
           DFPPTL LPLVYAGEKN+TAALCGEGSLKDIDVKGK VVCERGGGIARIAKG EVKNAGG
Sbjct: 361 DFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGG 420

Query: 421 AAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDN 480
           AAMILLNQQ DGF+TEADAHVLPA+HV+H AALKIK YINST  P A I F+GTVIGDD+
Sbjct: 421 AAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDD 480

Query: 481 FSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKN-RNTKSTFNIISGTSMS 540
           FSPAIASFSSRGPS+ASPGILKPDITGPG+SILAAWPFPLD N +NTK TFN+ISGTSMS
Sbjct: 481 FSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMS 540

Query: 541 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVN 600
           CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITN +G+PI+D+N QPADLFATGAGHVN
Sbjct: 541 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVN 600

Query: 601 PSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTV 660
           PSKA DPGLVYDIQPDDYIPYLCGLGYK NEV+ IA +P++C AKPSIPEG+LNYPSF+V
Sbjct: 601 PSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSV 660

Query: 661 VLGPPQTFTRTVTNVGCGREVYTAVVEAPPS-ISVTIRPSKIFFSKINQKVTFSVTFKRI 720
            LGPPQTFTRTVTNVG G EVYT ++EAPPS +SVT+RP KIFFS +NQKV +SVTFKRI
Sbjct: 661 ALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRI 720

Query: 721 GSIS-PSTEFGKGYLKWVSDKHVVRSPISFKF 738
           GSI+ PS+   + YLK +S KH+VRSPIS KF
Sbjct: 721 GSITPPSSGSAQAYLKCMSSKHIVRSPISIKF 749

BLAST of CmoCh02G015390.1 vs. NCBI nr
Match: XP_022958542.1 (subtilisin-like protease SBT1.8 [Cucurbita moschata])

HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 738/738 (100.00%), Postives = 738/738 (100.00%), Query Frame = 0

Query: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60
           MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL
Sbjct: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60

Query: 61  LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
           LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD
Sbjct: 61  LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120

Query: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
           SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT
Sbjct: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180

Query: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
           KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC
Sbjct: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240

Query: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
           FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA
Sbjct: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300

Query: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
           NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY
Sbjct: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360

Query: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
           AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG
Sbjct: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420

Query: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
           FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG
Sbjct: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480

Query: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
           PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS
Sbjct: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540

Query: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
           SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI
Sbjct: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600

Query: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
           QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT
Sbjct: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660

Query: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
           NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL
Sbjct: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720

Query: 721 KWVSDKHVVRSPISFKFA 739
           KWVSDKHVVRSPISFKFA
Sbjct: 721 KWVSDKHVVRSPISFKFA 738

BLAST of CmoCh02G015390.1 vs. NCBI nr
Match: KAG7036083.1 (Subtilisin-like protease SBT1.8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1465.3 bits (3792), Expect = 0.0e+00
Identity = 737/738 (99.86%), Postives = 737/738 (99.86%), Query Frame = 0

Query: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60
           MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL
Sbjct: 65  MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 124

Query: 61  LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
           LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD
Sbjct: 125 LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 184

Query: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
           SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT
Sbjct: 185 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 244

Query: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
           KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC
Sbjct: 245 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 304

Query: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
           FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA
Sbjct: 305 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 364

Query: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
           NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY
Sbjct: 365 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 424

Query: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
           AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG
Sbjct: 425 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 484

Query: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
           FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG
Sbjct: 485 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 544

Query: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
           PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS
Sbjct: 545 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 604

Query: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
           SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI
Sbjct: 605 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 664

Query: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
           QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT
Sbjct: 665 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 724

Query: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
           NVGCGREVYTAVVEA PSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL
Sbjct: 725 NVGCGREVYTAVVEASPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 784

Query: 721 KWVSDKHVVRSPISFKFA 739
           KWVSDKHVVRSPISFKFA
Sbjct: 785 KWVSDKHVVRSPISFKFA 802

BLAST of CmoCh02G015390.1 vs. NCBI nr
Match: KAG6606138.1 (Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1458.0 bits (3773), Expect = 0.0e+00
Identity = 734/738 (99.46%), Postives = 735/738 (99.59%), Query Frame = 0

Query: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60
           MVAFPSLFLLLLL+FHYVGALVTELPLINLQTYIVHVKKPETTDDLE WHRSFLPSSSSL
Sbjct: 1   MVAFPSLFLLLLLDFHYVGALVTELPLINLQTYIVHVKKPETTDDLESWHRSFLPSSSSL 60

Query: 61  LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
           LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD
Sbjct: 61  LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120

Query: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
           SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT
Sbjct: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180

Query: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
           KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC
Sbjct: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240

Query: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
           FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA
Sbjct: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300

Query: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
           NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEE DGESLFQPSDFPPTLLPLVY
Sbjct: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEECDGESLFQPSDFPPTLLPLVY 360

Query: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
           AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG
Sbjct: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420

Query: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
           FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG
Sbjct: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480

Query: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
           PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS
Sbjct: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540

Query: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
           SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI
Sbjct: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600

Query: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
           QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT
Sbjct: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660

Query: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
           NVGCGREVYTAVVEA PSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL
Sbjct: 661 NVGCGREVYTAVVEASPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720

Query: 721 KWVSDKHVVRSPISFKFA 739
           KWVSDKHVVRSPISFKFA
Sbjct: 721 KWVSDKHVVRSPISFKFA 738

BLAST of CmoCh02G015390.1 vs. NCBI nr
Match: XP_023532974.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1454.1 bits (3763), Expect = 0.0e+00
Identity = 729/738 (98.78%), Postives = 733/738 (99.32%), Query Frame = 0

Query: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60
           MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHV+KPETTDDLE WHRSFLPSSSSL
Sbjct: 1   MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVEKPETTDDLESWHRSFLPSSSSL 60

Query: 61  LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
           LYSYRNVMSGFAARLSEEQVKAMEE DGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD
Sbjct: 61  LYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120

Query: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
           SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT
Sbjct: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180

Query: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
           K LKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC
Sbjct: 181 KVLKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240

Query: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
           FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA
Sbjct: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300

Query: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
           NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPT LPLVY
Sbjct: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTFLPLVY 360

Query: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
           AGEKNQTAALCGEGSLKDIDVKGK+VVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG
Sbjct: 361 AGEKNQTAALCGEGSLKDIDVKGKIVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420

Query: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
           FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG
Sbjct: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480

Query: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
           PSVASPGILKPDITGPGVSILAAWPFPLDKN NTKSTFNIISGTSMSCPHLSGIAALIKS
Sbjct: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNSNTKSTFNIISGTSMSCPHLSGIAALIKS 540

Query: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
           SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI
Sbjct: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600

Query: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
           QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT
Sbjct: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660

Query: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
           NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKIN+KVT+SVTFKRIGSISPSTEFGKGYL
Sbjct: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINEKVTYSVTFKRIGSISPSTEFGKGYL 720

Query: 721 KWVSDKHVVRSPISFKFA 739
           KWVSDKHVVRSPISFKFA
Sbjct: 721 KWVSDKHVVRSPISFKFA 738

BLAST of CmoCh02G015390.1 vs. NCBI nr
Match: XP_023522248.1 (subtilisin-like protease SBT1.8, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1345.5 bits (3481), Expect = 0.0e+00
Identity = 671/678 (98.97%), Postives = 674/678 (99.41%), Query Frame = 0

Query: 61  LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
           LYSYRNVMSGFAARLSEEQVKAMEE DGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD
Sbjct: 1   LYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 60

Query: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
           SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT
Sbjct: 61  SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 120

Query: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
           K LKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC
Sbjct: 121 KVLKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 180

Query: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
           FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA
Sbjct: 181 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 240

Query: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
           NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPT LPLVY
Sbjct: 241 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTFLPLVY 300

Query: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
           AGEKNQTAALCGEGSLKDIDVKGK+VVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG
Sbjct: 301 AGEKNQTAALCGEGSLKDIDVKGKIVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 360

Query: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
           FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG
Sbjct: 361 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 420

Query: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
           PSVASPGILKPDITGPGVSILAAWPFPLDKN NTKSTFNIISGTSMSCPHLSGIAALIKS
Sbjct: 421 PSVASPGILKPDITGPGVSILAAWPFPLDKNSNTKSTFNIISGTSMSCPHLSGIAALIKS 480

Query: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
           SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI
Sbjct: 481 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 540

Query: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
           QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT
Sbjct: 541 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 600

Query: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
           NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKIN+KVT+SVTFKRIGSISPSTEFGKGYL
Sbjct: 601 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINEKVTYSVTFKRIGSISPSTEFGKGYL 660

Query: 721 KWVSDKHVVRSPISFKFA 739
           KWVSDKHVVRSPISFKFA
Sbjct: 661 KWVSDKHVVRSPISFKFA 678

BLAST of CmoCh02G015390.1 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 605.9 bits (1561), Expect = 4.3e-173
Identity = 339/730 (46.44%), Postives = 458/730 (62.74%), Query Frame = 0

Query: 31  QTYIV---HVKKPETTDDLEIWHRSFLPSSSSLLYSYRNVMSGFAARL-SEEQVKAMEEM 90
           +TYI+   H  KPE+      W+ S L S SSLLY+Y     GF+A L S E    +   
Sbjct: 28  KTYIIRVNHSDKPESFLTHHDWYTSQLNSESSLLYTYTTSFHGFSAYLDSTEADSLLSSS 87

Query: 91  DGFVSARRERILQLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDV 150
           +  +    + +  LHTT TP+FLGLN +FG     +   GVIIGVLD G+ P   SFDD 
Sbjct: 88  NSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDT 147

Query: 151 GMPPPPPKWKGRCE----FNFSACNNKLIGARSFNLATKALKGETMMDD----SPIDEDG 210
            MP  P KWKG CE    F+   CN KLIGARSF+   +   G          SP D DG
Sbjct: 148 DMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDG 207

Query: 211 HGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVCFGEDCPDTDILAALDAAI 270
           HGTHT++TAAG+ V+ A  LG A GTA GMA  A +A YKVC+   C  +DILAA+D AI
Sbjct: 208 HGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAMDRAI 267

Query: 271 EDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAANSGPFKATLSNEAPWILT 330
            DGVDVLSLSLG  S P+++D +AIGAF+A+++G+FVSCSA NSGP +A+++N APW++T
Sbjct: 268 LDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMT 327

Query: 331 VAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVYAGEKNQTAALCGEGSLKD 390
           V A T+DR   A A LGNG+   G SL+         L LVY    + ++ LC  GSL  
Sbjct: 328 VGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDS 387

Query: 391 IDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDGFSTEADAHVLPASHVSHK 450
             V+GK+VVC+RG   AR+ KG  V++AGG  MI+ N    G    AD+H+LPA  V  K
Sbjct: 388 SIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKK 447

Query: 451 AALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRGPSVASPGILKPDITGPGV 510
               ++ Y+ S + PTA ++FKGTV+ D   SP +A+FSSRGP+  +P ILKPD+ GPGV
Sbjct: 448 TGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGV 507

Query: 511 SILAAW-----PFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKSSHPDWSPAAIKSA 570
           +ILA W     P  LDK+ + ++ FNI+SGTSMSCPH+SG+A L+K++HP+WSP+AIKSA
Sbjct: 508 NILAGWSDAIGPTGLDKD-SRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSA 567

Query: 571 IMTTA---DITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDIQPDDYIPYLC 630
           +MTTA   D TN       D +L  ++ +A G+GHV+P KA  PGLVYDI  ++YI +LC
Sbjct: 568 LMTTAYVLDNTNAPLHDAADNSL--SNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLC 627

Query: 631 GLGYKSNEVATIARKP-INCLAKPSIPEGDLNYPSFTVVLGPPQT--FTRTVTNVGCGRE 690
            L Y  + +  I ++P +NC  K S P G LNYPSF+V+ G  +   +TR VTNVG    
Sbjct: 628 SLDYTVDHIVAIVKRPSVNCSKKFSDP-GQLNYPSFSVLFGGKRVVRYTREVTNVGAASS 687

Query: 691 VYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYLKWVSDKH 738
           VY   V   PS+ ++++PSK+ F  + +K  ++VTF     +S + +   G + W + +H
Sbjct: 688 VYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQH 747

BLAST of CmoCh02G015390.1 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 590.1 bits (1520), Expect = 2.4e-168
Identity = 344/747 (46.05%), Postives = 458/747 (61.31%), Query Frame = 0

Query: 30  LQTYIVHVK---KPETTDDLEIWHRSFL------PSSSSLLYSYRNVMSGFAARLSEEQV 89
           L++YIVHV+   KP        WH S L      P  ++LLYSY   + GF+ARLS  Q 
Sbjct: 30  LESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSYSRAVHGFSARLSPIQT 89

Query: 90  KAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPSH 149
            A+      +S   ++  ++HTTHTP FLG ++  G W +SN+G+ VI+GVLD GI P H
Sbjct: 90  AALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPEH 149

Query: 150 PSFDDVGMPPPPPKWKGRCE----FNFSACNNKLIGARSFNLATKALKGETMM-----DD 209
           PSF D G+ P P  WKG CE    F  S+CN KLIGAR+F       +  T         
Sbjct: 150 PSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESR 209

Query: 210 SPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVCFGEDCPDTDIL 269
           SP D +GHGTHTASTAAG+ V  A     A+GTA GMA  A +A YK+C+   C D+DIL
Sbjct: 210 SPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICWTGGCYDSDIL 269

Query: 270 AALDAAIEDGVDVLSLSLG-SPSVP-FFQDLVAIGAFAAIQKGIFVSCSAANSGPFKATL 329
           AA+D A+ DGV V+SLS+G S S P +  D +AIGAF A + GI VSCSA NSGP   T 
Sbjct: 270 AAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETA 329

Query: 330 SNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVYAGEKNQTAA 389
           +N APWILTV AST+DR   A A  G+G+ F G SL+     P + L LVY+G+    + 
Sbjct: 330 TNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVYSGDCG--SR 389

Query: 390 LCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDGFSTEADAHV 449
           LC  G L    V+GK+V+C+RGG  AR+ KG+ VK AGGA MIL N  + G    AD+H+
Sbjct: 390 LCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAESGEELTADSHL 449

Query: 450 LPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRGPSVASPGIL 509
           +PA+ V  KA  +I+ YI ++  PTA I F GT+IG    SP +A+FSSRGP+  +P IL
Sbjct: 450 VPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVIL 509

Query: 510 KPDITGPGVSILAAW-----PFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKSSHPD 569
           KPD+  PGV+ILA W     P  LD +   +  FNIISGTSMSCPH+SG+AAL++ +HPD
Sbjct: 510 KPDVIAPGVNILAGWTGMVGPTDLDIDPR-RVQFNIISGTSMSCPHVSGLAALLRKAHPD 569

Query: 570 WSPAAIKSAIMTTADITNLEGQPIVD-ENLQPADLFATGAGHVNPSKAADPGLVYDIQPD 629
           WSPAAIKSA++TTA      G+PI D    + ++ F  GAGHV+P+KA +PGLVYDI+  
Sbjct: 570 WSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVK 629

Query: 630 DYIPYLCGLGYKSNEVATIARKPI---NCLAKPSIPEGDLNYPSFTVVL---GPPQTFTR 689
           +Y+ +LC +GY+   +    + P     C        GDLNYPSF+VV    G    + R
Sbjct: 630 EYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKYKR 689

Query: 690 TVTNVGCGRE-VYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKR------IGSIS 738
            V NVG   + VY   V++P ++ + + PSK+ FSK    + + VTFK       +GS+ 
Sbjct: 690 VVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSV- 749

BLAST of CmoCh02G015390.1 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 582.4 bits (1500), Expect = 5.1e-166
Identity = 339/756 (44.84%), Postives = 464/756 (61.38%), Query Frame = 0

Query: 6   SLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEI---WHRSFLPS---SSS 65
           + FLLL L F +V +  ++       TYIVH+ K +     ++   W+ S L S   S+ 
Sbjct: 10  AFFLLLCLGFCHVSSSSSD-----QGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE 69

Query: 66  LLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFG-FW 125
           LLY+Y N + GF+ RL++E+  ++    G +S   E   +LHTT TP FLGL+      +
Sbjct: 70  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLF 129

Query: 126 KDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCE----FNFSACNNKLIGAR 185
            ++     V++GVLD G+ P   S+ D G  P P  WKG CE    F  S CN KLIGAR
Sbjct: 130 PEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGAR 189

Query: 186 SFNLATKALKG---ETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPL 245
            F    ++  G   E+    SP D+DGHGTHT+STAAG+ V+GA  LG A GTA GMAP 
Sbjct: 190 FFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPR 249

Query: 246 AHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQK 305
           A +A+YKVC+   C  +DILAA+D AI D V+VLS+SLG     +++D VAIGAFAA+++
Sbjct: 250 ARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMER 309

Query: 306 GIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDF 365
           GI VSCSA N+GP  ++LSN APWI TV A T+DR   A A LGNG+ F G SLF+    
Sbjct: 310 GILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL 369

Query: 366 PPTLLPLVYAGEKNQ--TAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGA 425
           P  LLP +YAG  +      LC  G+L    VKGK+V+C+RG   AR+ KG  VK AGG 
Sbjct: 370 PDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGGV 429

Query: 426 AMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNF 485
            MIL N   +G    ADAH+LPA+ V  KA   I+ Y+ +   PTA+I   GTV+G    
Sbjct: 430 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP- 489

Query: 486 SPAIASFSSRGPSVASPGILKPDITGPGVSILAAW-----PFPLDKNRNTKSTFNIISGT 545
           SP +A+FSSRGP+  +P ILKPD+  PGV+ILAAW     P  L  + + +  FNIISGT
Sbjct: 490 SPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASD-SRRVEFNIISGT 549

Query: 546 SMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVD-ENLQPADLFATGA 605
           SMSCPH+SG+AAL+KS HP+WSPAAI+SA+MTTA  T  +G+P++D    +P+  F  GA
Sbjct: 550 SMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGA 609

Query: 606 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYP 665
           GHV+P+ A +PGL+YD+  +DY+ +LC L Y S ++ +++R+   C    S    DLNYP
Sbjct: 610 GHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYP 669

Query: 666 SFTVVLG--PPQTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSV 725
           SF V +       +TRTVT+VG        V      + +++ P+ + F + N+K +++V
Sbjct: 670 SFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTV 729

Query: 726 TFKRIGSISPSTEFGKGYLKWVSDKHVVRSPISFKF 738
           TF  + S  PS     G ++W   KHVV SP++  +
Sbjct: 730 TF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of CmoCh02G015390.1 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 579.3 bits (1492), Expect = 4.3e-165
Identity = 334/756 (44.18%), Postives = 459/756 (60.71%), Query Frame = 0

Query: 27  LINLQTYIVHV----KKPETTDDLEIWHRSFLPS------------SSSLLYSYRNVMSG 86
           ++  QTYIV +    +  +T      WH SFL              SS LLYSY + + G
Sbjct: 22  ILQKQTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEG 81

Query: 87  FAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLN--RQFGFWKDSNFGKGVI 146
           FAA+L+E + + +      V+ R + +LQ+ TT++  FLGL+     G W  S FG+G I
Sbjct: 82  FAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTI 141

Query: 147 IGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCE----FNFSACNNKLIGARSF----NLAT 206
           IGVLD G+ P  PSFDD GMP  P KWKG C+    F+ S+CN KLIGAR F     +A 
Sbjct: 142 IGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFFIRGHRVAN 201

Query: 207 KALKGETMMDD--SPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYK 266
              +   M  +  S  D  GHGTHTAST  G+ V  A  LGN  G A GMAP AH+A+YK
Sbjct: 202 SPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYK 261

Query: 267 VCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCS 326
           VC+   C  +DILAA+D AI+D VDVLSLSLG   +P + D +AIG F A+++GI V C+
Sbjct: 262 VCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICA 321

Query: 327 AANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPT--LL 386
           A N+GP +++++N APW+ T+ A T+DRR  A  +L NG+   GESL+           +
Sbjct: 322 AGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREV 381

Query: 387 PLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQ 446
            ++Y    ++ +  C  GSL   +++GK+V+C+RG    R  KG  VK AGG AMIL N 
Sbjct: 382 EVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMILANT 441

Query: 447 QQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASF 506
           + +      D H+LPA+ + +  ++ +KAY+N+T  P A I+F GTVIG    +P +A F
Sbjct: 442 EINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGRSR-APEVAQF 501

Query: 507 SSRGPSVASPGILKPDITGPGVSILAAWP-------FPLDKNRNTKSTFNIISGTSMSCP 566
           S+RGPS+A+P ILKPD+  PGV+I+AAWP        P D  R     F ++SGTSMSCP
Sbjct: 502 SARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRR---VNFTVMSGTSMSCP 561

Query: 567 HLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPS 626
           H+SGI ALI+S++P+WSPAAIKSA+MTTAD+ + +G+ I D N +PA +FA GAGHVNP 
Sbjct: 562 HVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQ 621

Query: 627 KAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINC---LAKPSIPEGDLNYPSFT 686
           KA +PGLVY+IQP DYI YLC LG+  +++  I  K ++C   L K   P   LNYPS  
Sbjct: 622 KAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKN--PGFSLNYPSIA 681

Query: 687 VVLGPPQT---FTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTF 735
           V+    +T    TR VTNVG    +Y+  V+AP  I V + P ++ F  ++Q +++ V F
Sbjct: 682 VIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWF 741

BLAST of CmoCh02G015390.1 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 572.4 bits (1474), Expect = 5.3e-163
Identity = 337/746 (45.17%), Postives = 453/746 (60.72%), Query Frame = 0

Query: 29  NLQTYIVHV---KKPETTDDLEIWHRSFLPSSS----SLLYSYRNVMSGFAARLSEEQVK 88
           N  TYIVHV    KP        W+ S L S +    S++++Y  V  GF+ARL+ +   
Sbjct: 24  NSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARLTSQDAS 83

Query: 89  AMEEMDGFVSARRERILQLHTTHTPDFLGLNR--QFGFWKDSNFGKGVIIGVLDGGIAPS 148
            + +    +S   E++  LHTT +P+FLGL    + G  ++S+FG  ++IGV+D G+ P 
Sbjct: 84  QLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPE 143

Query: 149 HPSFDDVGMPPPPPKWKGRC----EFNFSACNNKLIGARSF---NLATKALKGETMMDDS 208
            PSFDD G+ P P KWKG+C    +F  SACN KL+GAR F     AT     ET    S
Sbjct: 144 RPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRS 203

Query: 209 PIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVCFGEDCPDTDILA 268
           P D DGHGTHTAS +AG +V  A  LG A G A GMAP A LA YKVC+   C D+DILA
Sbjct: 204 PRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILA 263

Query: 269 ALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAANSGPFKATLSNE 328
           A D A+ DGVDV+SLS+G   VP++ D +AIGAF AI +GIFVS SA N GP   T++N 
Sbjct: 264 AFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNV 323

Query: 329 APWILTVAASTIDRRIKAAAKLGNGEEFDGESLF-QPSDFPPTLLPLVYAGE----KNQT 388
           APW+ TV A TIDR   A  KLGNG+   G S++  P   P  + PLVY G        +
Sbjct: 324 APWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYS 383

Query: 389 AALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDGFSTEADA 448
           ++LC EGSL    VKGK+V+C+RG   +R  KG  V+  GG  MI+ N   DG    AD 
Sbjct: 384 SSLCLEGSLDPNLVKGKIVLCDRGIN-SRATKGEIVRKNGGLGMIIANGVFDGEGLVADC 443

Query: 449 HVLPASHVSHKAALKIKAYIN------STTYPTATILFKGTVIGDDNFSPAIASFSSRGP 508
           HVLPA+ V      +I+ YI+      S+ +PTATI+FKGT +G    +P +ASFS+RGP
Sbjct: 444 HVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLG-IRPAPVVASFSARGP 503

Query: 509 SVASPGILKPDITGPGVSILAAWPFPLD----KNRNTKSTFNIISGTSMSCPHLSGIAAL 568
           +  +P ILKPD+  PG++ILAAWP  +      + N ++ FNI+SGTSM+CPH+SG+AAL
Sbjct: 504 NPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAAL 563

Query: 569 IKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENL-QPADLFATGAGHVNPSKAADPGL 628
           +K++HPDWSPAAI+SA++TTA   +  G+P++DE+    + +   G+GHV+P+KA DPGL
Sbjct: 564 LKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPGL 623

Query: 629 VYDIQPDDYIPYLCGLGYKSNEVATIARKPINC-LAKPSIPEGDLNYPSFTVVLGP---- 688
           VYDI   DYI +LC   Y    + TI R+  +C  A+ +   G+LNYPSF+VV       
Sbjct: 624 VYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGES 683

Query: 689 --PQTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIG-S 734
                F RTVTNVG    VY   +  P   +VT+ P K+ F ++ QK++F V  K     
Sbjct: 684 KMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVK 743