Homology
BLAST of CmoCh02G015390 vs. ExPASy Swiss-Prot
Match:
A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)
HSP 1 Score: 965.3 bits (2494), Expect = 3.9e-280
Identity = 494/756 (65.34%), Postives = 583/756 (77.12%), Query Frame = 0
Query: 4 FPSLFLLLLLNFHYVGALVTELPLINLQT--------YIVHVKKPE-----TTDDLEIWH 63
F + L LL FH A +ELP ++ YI+HV PE ++DLE W+
Sbjct: 4 FLFIALTFLLTFHVHNAQGSELPTTTTESTETSSSKIYIIHVTGPEGKMLTESEDLESWY 63
Query: 64 RSFLP-------SSSSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTT 123
SFLP ++YSY+NV+ GFAA L++E++ A+E+ +GF+SA +R+L TT
Sbjct: 64 HSFLPPTLMSSEEQPRVIYSYKNVLRGFAASLTQEELSAVEKKNGFISAHPQRVLHRQTT 123
Query: 124 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNF 183
HTP FLGL + G WK+SNFGKGVIIGVLD GI P HPSF DVG+PPPPPKWKGRC+ N
Sbjct: 124 HTPKFLGLQQDTGVWKESNFGKGVIIGVLDSGITPGHPSFSDVGIPPPPPKWKGRCDLNV 183
Query: 184 SACNNKLIGARSFNLATKALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKG 243
+ACNNKLIGAR+FNLA +A+ G+ ++PIDEDGHGTHTASTAAGAFV AE LGNAKG
Sbjct: 184 TACNNKLIGARAFNLAAEAMNGKKA--EAPIDEDGHGTHTASTAAGAFVNYAEVLGNAKG 243
Query: 244 TAVGMAPLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLG-SPSVPFFQDLVA 303
TA GMAP AHLAIYKVCFGEDCP++DILAALDAA+EDGVDV+S+SLG S PFF D A
Sbjct: 244 TAAGMAPHAHLAIYKVCFGEDCPESDILAALDAAVEDGVDVISISLGLSEPPPFFNDSTA 303
Query: 304 IGAFAAIQKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDG 363
IGAFAA+QKGIFVSC+A NSGPF +++ N APWILTV ASTIDRRI A AKLGNG+EFDG
Sbjct: 304 IGAFAAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTVGASTIDRRIVATAKLGNGQEFDG 363
Query: 364 ESLFQPSDFPPTLLPLVYAGEK-NQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGT 423
ES+FQPS F PTLLPL YAG+ + +A C GSL D +GKVV+CERGGGIARIAKG
Sbjct: 364 ESVFQPSSFTPTLLPLAYAGKNGKEESAFCANGSLDDSAFRGKVVLCERGGGIARIAKGE 423
Query: 424 EVKNAGGAAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKG 483
EVK AGGAAMIL+N + + FS AD H LPA+HVS+ A ++IKAYINST PTATILFKG
Sbjct: 424 EVKRAGGAAMILMNDETNAFSLSADVHALPATHVSYAAGIEIKAYINSTATPTATILFKG 483
Query: 484 TVIGDDNFSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNII 543
TVIG ++ +PA+ASFSSRGP++ SPGILKPDI GPGV+ILAAWPFPL + ++K TFNI
Sbjct: 484 TVIG-NSLAPAVASFSSRGPNLPSPGILKPDIIGPGVNILAAWPFPLSNSTDSKLTFNIE 543
Query: 544 SGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFAT 603
SGTSMSCPHLSGIAAL+KSSHP WSPAAIKSAIMT+AD NL + IVDE LQP DLFAT
Sbjct: 544 SGTSMSCPHLSGIAALLKSSHPHWSPAAIKSAIMTSADTINLGNKLIVDETLQPTDLFAT 603
Query: 604 GAGHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLN 663
G+GHVNPS+A DPGLVYDIQPDDYIPYLCGLGY EV IA + I C A SIPEG+LN
Sbjct: 604 GSGHVNPSRANDPGLVYDIQPDDYIPYLCGLGYSETEVGIIAHRKIKCSA--SIPEGELN 663
Query: 664 YPSFTVVLGPPQTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSV 723
YPSF+V LG +TFTRTVTNVG Y +V AP + V ++P K+ FS++NQK T+SV
Sbjct: 664 YPSFSVELGSSKTFTRTVTNVGEAHSSYDLIVAAPQGVDVKVQPYKLNFSEVNQKETYSV 723
Query: 724 TFKRIGSISPSTEFGKGYLKWVSDKHVVRSPISFKF 738
TF R G + + E+ +G+LKWVS KH VRSPIS KF
Sbjct: 724 TFSRTGLGNKTQEYAQGFLKWVSTKHTVRSPISVKF 754
BLAST of CmoCh02G015390 vs. ExPASy Swiss-Prot
Match:
A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)
HSP 1 Score: 858.6 bits (2217), Expect = 5.2e-248
Identity = 449/725 (61.93%), Postives = 549/725 (75.72%), Query Frame = 0
Query: 29 NLQTYIVHVKKPE------TTDDLEIWHRSFLPSSSS---LLYSYRNVMSGFAARLSEEQ 88
NL TYIVHVKK E +T++L WH SFLP +S+ +++SYRNV SGFA RL+ E+
Sbjct: 37 NLTTYIVHVKKLEIEGPLQSTEELHTWHHSFLPETSNKDRMVFSYRNVASGFAVRLTPEE 96
Query: 89 VKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPS 148
A++E + +S R ER L LHTTHTP FLGL + G W DSN GKGVIIGV+D GI P
Sbjct: 97 ANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDTGIYPF 156
Query: 149 HPSFDDVGMPPPPPKWKGRCEF-NFSACNNKLIGARSFNLATKALKGETMMDDSPIDEDG 208
H SF+D GMPPPP KWKG CEF S CNNKLIGAR NL A++ + P ++
Sbjct: 157 HLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGAR--NLVKSAIQ------EPPYEDFF 216
Query: 209 HGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVCFG---EDCPDTDILAALD 268
HGTHTA+ AAG FV+GA GNA+GTA GMAP AHLAIYKVC ++CP++ ILAA+D
Sbjct: 217 HGTHTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAILAAMD 276
Query: 269 AAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAANSGPFKATLSNEAPW 328
AIEDGVDVLSLSLG S+PFF+D +AIGAFAA QKGIFVSCSAANSGP ++LSNEAPW
Sbjct: 277 IAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSNEAPW 336
Query: 329 ILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY-AGEKNQTAALCGEG 388
ILTV ASTIDR+I A+AKLGNG E++GE+LFQP DF LLPLVY A EKN ++ALC G
Sbjct: 337 ILTVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSALCAPG 396
Query: 389 SLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDGFSTEADAHVLPASH 448
SL++I+VKGKVVVC+ GGGI IAKG EV +AGG+AMIL N + GF+T A+AHVLPA H
Sbjct: 397 SLRNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHVLPAVH 456
Query: 449 VSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRGPSVASPGILKPDIT 508
VS+ A+L IKAYINST PTAT+LF+GT+IG D+ +P++A+FSSRGPS SPGILKPDI
Sbjct: 457 VSYAASLAIKAYINSTYTPTATVLFQGTIIG-DSLAPSVAAFSSRGPSQQSPGILKPDII 516
Query: 509 GPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAI 568
GPGV+ILAAW +D N F+IISGTSMSCPHLSGIAAL+KS+HPDWSPAAIKSAI
Sbjct: 517 GPGVNILAAWAVSVD---NKIPAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAIKSAI 576
Query: 569 MTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLGY 628
MTTA+ NL G PI+D+ LQPAD+FATGAGHVNP +A DPGLVYDIQP+DY+PYLCGLGY
Sbjct: 577 MTTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLCGLGY 636
Query: 629 KSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLG-PPQTFTRTVTNVGCGREVYTAVV 688
EV I ++ + C SI + +LNYPSF+++LG Q +TRT+TNVG YT +
Sbjct: 637 SDREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTYTVKI 696
Query: 689 EAPPSISVTIRPSKIFFSKINQKVTFSVTF-KRIGSISPSTEFGKGYLKWVSDKHVVRSP 738
+ P ++ +++ PS+I F+++NQKV + V F +I + F +G + WVSDKHVVR+P
Sbjct: 697 DVPLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHVVRTP 749
BLAST of CmoCh02G015390 vs. ExPASy Swiss-Prot
Match:
A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)
HSP 1 Score: 858.2 bits (2216), Expect = 6.8e-248
Identity = 445/742 (59.97%), Postives = 552/742 (74.39%), Query Frame = 0
Query: 9 LLLLLNFHYVGALVTELPLINLQTYIVHVKKPE-----TTDDLEIWHRSFLPSS------ 68
+ LL+FH L T LQTYIVHV KP+ + DLE ++ SFLP++
Sbjct: 9 IAFLLSFH----LTTAQRSTGLQTYIVHVDKPDAQVLANSADLESYYNSFLPATVSGSEV 68
Query: 69 -SSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFG 128
S +++SY +V +GFAA+LS E+VK ME+ GFVSA+ E++L LHTTHTP+FLGL + G
Sbjct: 69 PSRIIHSYHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLGLYQNMG 128
Query: 129 FWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSA-CNNKLIGARS 188
FW++SN+GKGVIIG+LD GI P HPSF DV MP PP KWKG+CEF +A CN K+IGAR+
Sbjct: 129 FWQESNYGKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFTGNATCNKKIIGARN 188
Query: 189 FNLATKALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLA 248
F + G + P DE+GHGTHTASTAAG FV A GNA GTAVGMAPLAH+A
Sbjct: 189 F------ISGSGV---PPTDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGMAPLAHIA 248
Query: 249 IYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFV 308
+YKVC + C D DILAALDAAI+DGVDVLSLSLG S PF+ D +AIGAFAAI+KGIFV
Sbjct: 249 MYKVCSEDGCSDADILAALDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAAIRKGIFV 308
Query: 309 SCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTL 368
S SA N GP +TLSNEAPWILTV AST DR+I A A LGNG+++DGES FQP+DFP TL
Sbjct: 309 SASAGNDGPLNSTLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESAFQPADFPHTL 368
Query: 369 LPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLN 428
LPLVY G ++ AA C GSL DVKGKVVVC+RGG +AR+ K VK+AGGAAMIL N
Sbjct: 369 LPLVYPGTSDEEAAFCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAGGAAMILAN 428
Query: 429 QQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIAS 488
+ DG T ADAHVLPA+HV + A IK+YINST+ PTA ILFKGT+IG + SP+++S
Sbjct: 429 LEIDGEGTFADAHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTIIGFKS-SPSVSS 488
Query: 489 FSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIA 548
FSSRGP++ASPGI+KPDI GPGV+ILAAWP ++ T TFNIISGTSMSCPHLSGI
Sbjct: 489 FSSRGPNLASPGIVKPDIIGPGVNILAAWPVSVENKTGTDLTFNIISGTSMSCPHLSGIV 548
Query: 549 ALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPG 608
AL+KS+HPDWSPAAIKSAIMT+AD +NLEGQPI+DE PAD+FATGAGHVNPSKA+DPG
Sbjct: 549 ALLKSAHPDWSPAAIKSAIMTSADQSNLEGQPILDERNLPADIFATGAGHVNPSKASDPG 608
Query: 609 LVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGP-PQT 668
L+YDIQ +DYI YLCGLGY+ ++ I ++ + C + SI E +LNYPSF+++LGP Q
Sbjct: 609 LIYDIQLEDYIQYLCGLGYREKDIGLIVQETVKC--ESSISEAELNYPSFSIILGPKTQN 668
Query: 669 FTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTE 728
+TRTVTNVG YT + + + + P+ + F+K+ Q+ T++V+F + G +
Sbjct: 669 YTRTVTNVGDASSTYTVNIAKTQGVDIVVEPATLVFTKMYQQATYTVSFTQSGDF--TDR 728
Query: 729 FGKGYLKWVSDKHVVRSPISFK 737
F +G + W S+++VVRSPIS K
Sbjct: 729 FVQGAISWSSNEYVVRSPISVK 732
BLAST of CmoCh02G015390 vs. ExPASy Swiss-Prot
Match:
A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)
HSP 1 Score: 825.1 bits (2130), Expect = 6.3e-238
Identity = 430/720 (59.72%), Postives = 529/720 (73.47%), Query Frame = 0
Query: 29 NLQTYIVHVKKPET--TDDLEIWHRSFLPSSSS-----LLYSYRNVMSGFAARLSEEQVK 88
NL TYIVHV+KP+ +DDL ++ S LP S+ ++++YRNV++GFA +L+ E+ K
Sbjct: 41 NLSTYIVHVRKPQVIQSDDLHTFYYSLLPESTKTTNQRIVFTYRNVVNGFAVKLTPEEAK 100
Query: 89 AMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPSHP 148
A+++ + VSAR E+IL LHTTHTP FLGL + G WK SN GKGVIIG+LD GI+P HP
Sbjct: 101 ALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKGVIIGILDTGISPFHP 160
Query: 149 SFDDVGMPPPPPKWKGRCEFNFS-ACNNKLIGARSFNLATKALKGETMMDDSPIDEDGHG 208
SF D GMP PP KW G CEF CNNK+IGAR+F + TK L P D+ GHG
Sbjct: 161 SFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNF-VKTKNL-------TLPFDDVGHG 220
Query: 209 THTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVCFGEDCPDTDILAALDAAIED 268
THTASTAAG V+GA GNA GTAVGMAP AH+A+YKVC C ++ ILA +D A++D
Sbjct: 221 THTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAILAGMDTAVDD 280
Query: 269 GVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAANSGPFKATLSNEAPWILTVA 328
GVDVLSLSLG PS PFF+D +A+GAF AIQKGIFVSCSAANSGP ++LSNEAPWILTV
Sbjct: 281 GVDVLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLSNEAPWILTVG 340
Query: 329 ASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVYAGE--KNQTAALCGEGSLKD 388
AS+IDR I A AKLGNG+E+ G+S+FQP DF P+LLPLVYAG N + C SL
Sbjct: 341 ASSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGANGNNNFSVFCAPESLNR 400
Query: 389 IDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDGFSTEADAHVLPASHVSHK 448
DV+GKVV+CE GG + R+ KG VK+AGGAAMIL+N + F+ AD HVLPA H+S++
Sbjct: 401 SDVEGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADVHVLPAVHISYE 460
Query: 449 AALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRGPSVASPGILKPDITGPGV 508
A L +K YINST+ PTATILF+GTVIG + +P + SFSSRGPS ASPGILKPDI GPG+
Sbjct: 461 AGLALKEYINSTSTPTATILFEGTVIG-NLLAPQVTSFSSRGPSKASPGILKPDIIGPGL 520
Query: 509 SILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTA 568
+ILAAWP LD +T FNIISGTSMSCPHLSGIAAL+K+SHPDWSPAAIKSAIMTTA
Sbjct: 521 NILAAWPVSLD--NSTTPPFNIISGTSMSCPHLSGIAALLKNSHPDWSPAAIKSAIMTTA 580
Query: 569 DITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKSNE 628
NL G PI+D+ L PAD+FATGAGHVNP KA DPGLVYDI+P+DYIPYLCGL Y E
Sbjct: 581 SQVNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPNDYIPYLCGLNYTDRE 640
Query: 629 VATIARKPINCLAKPSIPEGDLNYPSFTVVLG-PPQTFTRTVTNVGCGREVYTAVVEAPP 688
V I ++ + C I E +LNYPSF+++LG Q +TRTV NVG YTA + P
Sbjct: 641 VGVILQQRVRCSEVNHIAEAELNYPSFSILLGNTTQLYTRTVANVGPANSTYTAEIGVPV 700
Query: 689 SISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYLKWVSDKHVVRSPISFKF 738
+ +++ P+++ F+++ QK+T+SV+F + F +G LKWVS K+ VRSPISF F
Sbjct: 701 GVGMSLSPAQLTFTEVGQKLTYSVSFIPFSEDRDNHTFAQGSLKWVSGKYSVRSPISFIF 749
BLAST of CmoCh02G015390 vs. ExPASy Swiss-Prot
Match:
G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)
HSP 1 Score: 816.2 bits (2107), Expect = 2.9e-235
Identity = 434/728 (59.62%), Postives = 527/728 (72.39%), Query Frame = 0
Query: 28 INLQTYIVHVKKPET-----TDDLEIWHRSFLPSS----SSLLYSYRNVMSGFAARLSEE 87
INL TYIVHVKK E ++DL W+ SFLP + +++SYR V SGFA +L+ E
Sbjct: 33 INLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQTFPHKERMVFSYRKVASGFAVKLTPE 92
Query: 88 QVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAP 147
+ K+++E VSAR ER L+LHTTHTP FLGL + G W D N GKGVIIG++D GI P
Sbjct: 93 EAKSLQEKGEIVSARPERTLELHTTHTPTFLGLKQGQGLWSDDNLGKGVIIGIIDTGIFP 152
Query: 148 SHPSFDDVGMPPPPPKWKGRCEF-NFSACNNKLIGARSFNLATKALKGETMMDDSPIDED 207
HPSF+D GMPPPP KWKG CEF CNNKLIGAR NL A++ + P +
Sbjct: 153 LHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLIGAR--NLVKSAIQ------EPPFENF 212
Query: 208 GHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVCFGE-DCPDTDILAALDA 267
HGTHTA+ AAG F++ A GNAKG A GMAP AHLAIYKVC + C ++ ILAA+D
Sbjct: 213 FHGTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNAHLAIYKVCNDKIGCTESAILAAMDI 272
Query: 268 AIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAANSGPFKATLSNEAPWI 327
AIEDGVDVLSLSLG S+PFF+D +AIGAFAA Q G+FVSCSAANSGP +TLSNEAPWI
Sbjct: 273 AIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQNGVFVSCSAANSGPGYSTLSNEAPWI 332
Query: 328 LTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVYA-----GEKNQTAALC 387
LTV ASTIDR+I A+AKLGNGEE++GE+LFQP DF LLPLVY G + Q +LC
Sbjct: 333 LTVGASTIDRKIVASAKLGNGEEYEGETLFQPKDFSQQLLPLVYPGSFGYGNQTQNQSLC 392
Query: 388 GEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDGFSTEADAHVLP 447
GSLK+ID+ GKVV+C+ G ++ I KG EV N+GG AMIL N + GFST A AHVLP
Sbjct: 393 LPGSLKNIDLSGKVVLCD-VGNVSSIVKGQEVLNSGGIAMILANSEALGFSTFAIAHVLP 452
Query: 448 ASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRGPSVASPGILKP 507
A VS+ A L IK+YI ST PTAT++FKGT+IG D+ +P++ FSSRGPS SPGILKP
Sbjct: 453 AVEVSYAAGLTIKSYIKSTYNPTATLIFKGTIIG-DSLAPSVVYFSSRGPSQESPGILKP 512
Query: 508 DITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKSSHPDWSPAAIK 567
DI GPGV+ILAAW +D N F+I+SGTSMSCPHLSGIAALIKSSHPDWSPAAIK
Sbjct: 513 DIIGPGVNILAAWAVSVD---NKIPAFDIVSGTSMSCPHLSGIAALIKSSHPDWSPAAIK 572
Query: 568 SAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDIQPDDYIPYLCG 627
SAIMTTA+ NL G PI+D+ L PAD+FATGAGHVNP KA DPGLVYDI+P+DY+PYLCG
Sbjct: 573 SAIMTTANTLNLGGIPILDQRLFPADIFATGAGHVNPVKANDPGLVYDIEPEDYVPYLCG 632
Query: 628 LGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLG-PPQTFTRTVTNVGCGREVYT 687
LGY E+ I + + C SIPE LNYPSF+++LG Q +TRT+TNVG Y
Sbjct: 633 LGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYPSFSILLGSDSQYYTRTLTNVGFANSTYK 692
Query: 688 AVVEAPPSISVTIRPSKIFFSKINQKVTFSVTF-KRIGSISPSTEFGKGYLKWVSDKHVV 738
+E P ++ +++ PS+I F+++N+KV+FSV F +I + FG+G L WVSD+H V
Sbjct: 693 VELEVPLALGMSVNPSEITFTEVNEKVSFSVEFIPQIKENRRNHTFGQGSLTWVSDRHAV 747
BLAST of CmoCh02G015390 vs. ExPASy TrEMBL
Match:
A0A6J1H242 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111459745 PE=3 SV=1)
HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 738/738 (100.00%), Postives = 738/738 (100.00%), Query Frame = 0
Query: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60
MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL
Sbjct: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60
Query: 61 LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD
Sbjct: 61 LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
Query: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT
Sbjct: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
Query: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC
Sbjct: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
Query: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA
Sbjct: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
Query: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY
Sbjct: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
Query: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG
Sbjct: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
Query: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG
Sbjct: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
Query: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS
Sbjct: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
Query: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI
Sbjct: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
Query: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT
Sbjct: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
Query: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL
Sbjct: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
Query: 721 KWVSDKHVVRSPISFKFA 739
KWVSDKHVVRSPISFKFA
Sbjct: 721 KWVSDKHVVRSPISFKFA 738
BLAST of CmoCh02G015390 vs. ExPASy TrEMBL
Match:
A0A5A7TJ55 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003250 PE=3 SV=1)
HSP 1 Score: 1238.4 bits (3203), Expect = 0.0e+00
Identity = 623/745 (83.62%), Postives = 663/745 (88.99%), Query Frame = 0
Query: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSS--- 60
MV PSLFLLLLLNFH A VTELPL NL TYIVHVKKPE DDLEIWHRSFLP+S
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 ----SSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNR 120
+LLYSYRNVMSGF+ARL+EE VKAMEE DGFVSARRE I+ LHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGI P+HPSFDD GMP PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
RS NLA++ALKG+ T +DDSPIDEDGHGTHTASTAAG FV GAEALGNA GTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
HLAIYKVCFGEDC D DILA LDAA+EDGVDVLS+SLG PSVPFF D+ AIG+FAAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
IFVSCSAANSGPF ATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 PTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMI 420
TLLPLV+ GEKN+T ALC EGSLK+IDVKGKVVVCERGGGIARIAKG EVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
LLN + DGF+TE DAHVLPASHVSH AALKIKAYINSTTYPTATILFKGT IGDD FSPA
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDD-FSPA 480
Query: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLS 540
IASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFNIISGTSMSCPHLS
Sbjct: 481 IASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLS 540
Query: 541 GIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAA 600
GIAALIKS+HPDWSPAAIKS+IMTTA+ITNLEG PI+DE LQPADLFA GAGHVNPSKA
Sbjct: 541 GIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAI 600
Query: 601 DPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPP 660
DPGLVYDIQPDDYIPYLCGLGY +N+V+ IA KPI+CL SIPEG+LNYPSF V LGP
Sbjct: 601 DPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPV 660
Query: 661 QTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPS 720
QTF+RTVT+VG GR VY V+EAP +SVT+RP K+ FS +NQK T+SVTFKRIGSISPS
Sbjct: 661 QTFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPS 720
Query: 721 TEFGKGYLKWVSDKHVVRSPISFKF 738
EF +GYLKWVS KHVVRSPIS KF
Sbjct: 721 IEFAEGYLKWVSAKHVVRSPISVKF 744
BLAST of CmoCh02G015390 vs. ExPASy TrEMBL
Match:
A0A1S3ATZ7 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482686 PE=3 SV=1)
HSP 1 Score: 1233.0 bits (3189), Expect = 0.0e+00
Identity = 621/745 (83.36%), Postives = 661/745 (88.72%), Query Frame = 0
Query: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSS--- 60
MV PSLFLLLLLNFH A VTELPL NL TYIVHVKKPE DDLEIWHRSFLP+S
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 ----SSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNR 120
+LLYSYRNVMSGF+ARL+EE VKAMEE DGFVSARRE I+ LHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGI P+HPSFDD GM PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
RS NLA++ALKG+ T +DDSPIDEDGHGTHTASTAAG FV GAEALGNA GTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
HLAIYKVCFGEDC D DILA LDAA+EDGVDVLS+SLG PSVPFF D+ AIG+FAAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
IFVSCSAANSGPF ATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 PTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMI 420
TLLPLV+ GEKN+T ALC EGSLK+IDVKGKVVVCERGGGIARIAKG EVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
LLN + DGF+TE DAHVLPASHVSH AALKIKAYINSTTYPTATILFKGT IGDD FSPA
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDD-FSPA 480
Query: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLS 540
IASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFNIISGTSMSCPHLS
Sbjct: 481 IASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLS 540
Query: 541 GIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAA 600
GIAALIKS+HPDWSPAAIKS+IMTTA+ITNLEG PI+DE LQPADLFA GAGHVNPSKA
Sbjct: 541 GIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAI 600
Query: 601 DPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPP 660
DPGLVYDIQPDDYIPYLCGLGY +N+V+ IA KPI+CL SIPEG+LNYPSF V LGP
Sbjct: 601 DPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPV 660
Query: 661 QTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPS 720
QTF+RTVT+VG GR VY V+EAP +SVT+RP K+ FS +NQK T+SVTFKR GSISPS
Sbjct: 661 QTFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRSGSISPS 720
Query: 721 TEFGKGYLKWVSDKHVVRSPISFKF 738
EF +GYLKWVS KHVVRSPIS KF
Sbjct: 721 IEFAEGYLKWVSAKHVVRSPISVKF 744
BLAST of CmoCh02G015390 vs. ExPASy TrEMBL
Match:
A0A0A0KKE3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157240 PE=3 SV=1)
HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 614/745 (82.42%), Postives = 659/745 (88.46%), Query Frame = 0
Query: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSS--- 60
MV PSLFLLLLLNFH A VTELP NL TYIVHVKKPE DDLE WHRSFLP+S
Sbjct: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
Query: 61 ----SSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNR 120
+LLYSYRNVMSGF+ARL+EE VKAMEE DGFVSARRE I+ LHTTH+P+FLGLNR
Sbjct: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGI PSHPSF D GMP PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
RS NLA++ALKG+ T +DDSPIDEDGHGTHTASTAAG FV GAEALGNA GTAVGMAPLA
Sbjct: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
HLAIYKVCFGE C + DILA LDAA+EDGVDVLS+SLG P VPFF D+ AIGAFAAIQKG
Sbjct: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
Query: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
IFVSCSAANSGPF ATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 PTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMI 420
T LPLV+ GEKN+T ALC EGSLK+IDVKGKVVVC+RGGGIARIAKG EVKNAGGAAMI
Sbjct: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
Query: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
LLN + DGF+TEADAHVLPASHVSH AALKIKAYINSTTYPTATI+FKGT IGDD FSPA
Sbjct: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDD-FSPA 480
Query: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLS 540
IA+FSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFNI+SGTSMSCPHLS
Sbjct: 481 IAAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLS 540
Query: 541 GIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAA 600
GIAALIKS+HPDWSPAAIKS+IMTTA+ITNLEG PIVD+ LQPADLFA GAGHVNPSKA
Sbjct: 541 GIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAV 600
Query: 601 DPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPP 660
DPGLVYDIQPDDYIPYLCGLGY +N+V+ IA KPI+CL SIPEG+LNYPSF V LG
Sbjct: 601 DPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQV 660
Query: 661 QTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPS 720
QTF+RTVT VG GREVY V+EAP +SVT+RP K+ FS +NQK T+SVTFKRIGSISPS
Sbjct: 661 QTFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPS 720
Query: 721 TEFGKGYLKWVSDKHVVRSPISFKF 738
TEF +GYLKWVS KH+VRSPIS KF
Sbjct: 721 TEFAEGYLKWVSAKHLVRSPISVKF 744
BLAST of CmoCh02G015390 vs. ExPASy TrEMBL
Match:
A0A6J1E0E3 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111025997 PE=3 SV=1)
HSP 1 Score: 1213.7 bits (3139), Expect = 0.0e+00
Identity = 622/752 (82.71%), Postives = 667/752 (88.70%), Query Frame = 0
Query: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPE------TTDDLEIWHRSFL 60
MV PSLFLL+L +FH GA T NLQTYIVHVKKPE +DLE WHRSFL
Sbjct: 1 MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60
Query: 61 PSSS----SLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLG 120
PSSS LLYS+ NVMSGFAARL+EE VKAMEE DGF+SARRERIL+LHTTHTP+FLG
Sbjct: 61 PSSSEDHPKLLYSFXNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPNFLG 120
Query: 121 LNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKL 180
LNRQFGFWKDSNFGKG IIGVLDGGI P+HPSFDD GMPPPP KWKGRCEFNFSACNNKL
Sbjct: 121 LNRQFGFWKDSNFGKGXIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACNNKL 180
Query: 181 IGARSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMA 240
IGARSFNLATKALKGE DSPIDEDGHGTHTASTAAGAFV AEALGNAKGTAVGMA
Sbjct: 181 IGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAVGMA 240
Query: 241 PLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAI 300
PLAHLAIYKVCFGEDCPD+DILAALDAA+EDGVDVLSLSLGS S PFF+D +AIGAFAA
Sbjct: 241 PLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAFAAT 300
Query: 301 QKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPS 360
QKGIFVSCSA+NSGP ATLSNEAPWILTVAASTIDRRI A AKLGNGEEFDGESLFQPS
Sbjct: 301 QKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLFQPS 360
Query: 361 DFPPTL-LPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGG 420
DFPPTL LPLVYAGEKN+TAALCGEGSLKDIDVKGK VVCERGGGIARIAKG EVKNAGG
Sbjct: 361 DFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKNAGG 420
Query: 421 AAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDN 480
AAMILLNQQ DGF+TEADAHVLPA+HV+H AALKIK YINST P A I F+GTVIGDD+
Sbjct: 421 AAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIGDDD 480
Query: 481 FSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKN-RNTKSTFNIISGTSMS 540
FSPAIASFSSRGPS+ASPGILKPDITGPG+SILAAWPFPLD N +NTK TFN+ISGTSMS
Sbjct: 481 FSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGTSMS 540
Query: 541 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVN 600
CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITN +G+PI+D+N QPADLFATGAGHVN
Sbjct: 541 CPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAGHVN 600
Query: 601 PSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTV 660
PSKA DPGLVYDIQPDDYIPYLCGLGYK NEV+ IA +P++C AKPSIPEG+LNYPSF+V
Sbjct: 601 PSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPSFSV 660
Query: 661 VLGPPQTFTRTVTNVGCGREVYTAVVEAPPS-ISVTIRPSKIFFSKINQKVTFSVTFKRI 720
LGPPQTFTRTVTNVG G EVYT ++EAPPS +SVT+RP KIFFS +NQKV +SVTFKRI
Sbjct: 661 ALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTFKRI 720
Query: 721 GSIS-PSTEFGKGYLKWVSDKHVVRSPISFKF 738
GSI+ PS+ + YLK +S KH+VRSPIS KF
Sbjct: 721 GSITPPSSGSAQAYLKCMSSKHIVRSPISIKF 749
BLAST of CmoCh02G015390 vs. NCBI nr
Match:
XP_022958542.1 (subtilisin-like protease SBT1.8 [Cucurbita moschata])
HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 738/738 (100.00%), Postives = 738/738 (100.00%), Query Frame = 0
Query: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60
MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL
Sbjct: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60
Query: 61 LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD
Sbjct: 61 LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
Query: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT
Sbjct: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
Query: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC
Sbjct: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
Query: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA
Sbjct: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
Query: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY
Sbjct: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
Query: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG
Sbjct: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
Query: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG
Sbjct: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
Query: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS
Sbjct: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
Query: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI
Sbjct: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
Query: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT
Sbjct: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
Query: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL
Sbjct: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
Query: 721 KWVSDKHVVRSPISFKFA 739
KWVSDKHVVRSPISFKFA
Sbjct: 721 KWVSDKHVVRSPISFKFA 738
BLAST of CmoCh02G015390 vs. NCBI nr
Match:
KAG7036083.1 (Subtilisin-like protease SBT1.8, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1465.3 bits (3792), Expect = 0.0e+00
Identity = 737/738 (99.86%), Postives = 737/738 (99.86%), Query Frame = 0
Query: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60
MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL
Sbjct: 65 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 124
Query: 61 LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD
Sbjct: 125 LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 184
Query: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT
Sbjct: 185 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 244
Query: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC
Sbjct: 245 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 304
Query: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA
Sbjct: 305 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 364
Query: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY
Sbjct: 365 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 424
Query: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG
Sbjct: 425 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 484
Query: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG
Sbjct: 485 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 544
Query: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS
Sbjct: 545 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 604
Query: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI
Sbjct: 605 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 664
Query: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT
Sbjct: 665 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 724
Query: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
NVGCGREVYTAVVEA PSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL
Sbjct: 725 NVGCGREVYTAVVEASPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 784
Query: 721 KWVSDKHVVRSPISFKFA 739
KWVSDKHVVRSPISFKFA
Sbjct: 785 KWVSDKHVVRSPISFKFA 802
BLAST of CmoCh02G015390 vs. NCBI nr
Match:
KAG6606138.1 (Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1458.0 bits (3773), Expect = 0.0e+00
Identity = 734/738 (99.46%), Postives = 735/738 (99.59%), Query Frame = 0
Query: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60
MVAFPSLFLLLLL+FHYVGALVTELPLINLQTYIVHVKKPETTDDLE WHRSFLPSSSSL
Sbjct: 1 MVAFPSLFLLLLLDFHYVGALVTELPLINLQTYIVHVKKPETTDDLESWHRSFLPSSSSL 60
Query: 61 LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD
Sbjct: 61 LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
Query: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT
Sbjct: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
Query: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC
Sbjct: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
Query: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA
Sbjct: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
Query: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEE DGESLFQPSDFPPTLLPLVY
Sbjct: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEECDGESLFQPSDFPPTLLPLVY 360
Query: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG
Sbjct: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
Query: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG
Sbjct: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
Query: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS
Sbjct: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
Query: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI
Sbjct: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
Query: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT
Sbjct: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
Query: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
NVGCGREVYTAVVEA PSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL
Sbjct: 661 NVGCGREVYTAVVEASPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
Query: 721 KWVSDKHVVRSPISFKFA 739
KWVSDKHVVRSPISFKFA
Sbjct: 721 KWVSDKHVVRSPISFKFA 738
BLAST of CmoCh02G015390 vs. NCBI nr
Match:
XP_023532974.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1454.1 bits (3763), Expect = 0.0e+00
Identity = 729/738 (98.78%), Postives = 733/738 (99.32%), Query Frame = 0
Query: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSSL 60
MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHV+KPETTDDLE WHRSFLPSSSSL
Sbjct: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVEKPETTDDLESWHRSFLPSSSSL 60
Query: 61 LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
LYSYRNVMSGFAARLSEEQVKAMEE DGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD
Sbjct: 61 LYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
Query: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT
Sbjct: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
Query: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
K LKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC
Sbjct: 181 KVLKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
Query: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA
Sbjct: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
Query: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPT LPLVY
Sbjct: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTFLPLVY 360
Query: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
AGEKNQTAALCGEGSLKDIDVKGK+VVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG
Sbjct: 361 AGEKNQTAALCGEGSLKDIDVKGKIVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
Query: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG
Sbjct: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
Query: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
PSVASPGILKPDITGPGVSILAAWPFPLDKN NTKSTFNIISGTSMSCPHLSGIAALIKS
Sbjct: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNSNTKSTFNIISGTSMSCPHLSGIAALIKS 540
Query: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI
Sbjct: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
Query: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT
Sbjct: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
Query: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKIN+KVT+SVTFKRIGSISPSTEFGKGYL
Sbjct: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINEKVTYSVTFKRIGSISPSTEFGKGYL 720
Query: 721 KWVSDKHVVRSPISFKFA 739
KWVSDKHVVRSPISFKFA
Sbjct: 721 KWVSDKHVVRSPISFKFA 738
BLAST of CmoCh02G015390 vs. NCBI nr
Match:
XP_023522248.1 (subtilisin-like protease SBT1.8, partial [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1345.5 bits (3481), Expect = 0.0e+00
Identity = 671/678 (98.97%), Postives = 674/678 (99.41%), Query Frame = 0
Query: 61 LYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 120
LYSYRNVMSGFAARLSEEQVKAMEE DGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD
Sbjct: 1 LYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKD 60
Query: 121 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 180
SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT
Sbjct: 61 SNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGARSFNLAT 120
Query: 181 KALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 240
K LKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC
Sbjct: 121 KVLKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVC 180
Query: 241 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 300
FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA
Sbjct: 181 FGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAA 240
Query: 301 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVY 360
NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPT LPLVY
Sbjct: 241 NSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTFLPLVY 300
Query: 361 AGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 420
AGEKNQTAALCGEGSLKDIDVKGK+VVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG
Sbjct: 301 AGEKNQTAALCGEGSLKDIDVKGKIVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDG 360
Query: 421 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 480
FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG
Sbjct: 361 FSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRG 420
Query: 481 PSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKS 540
PSVASPGILKPDITGPGVSILAAWPFPLDKN NTKSTFNIISGTSMSCPHLSGIAALIKS
Sbjct: 421 PSVASPGILKPDITGPGVSILAAWPFPLDKNSNTKSTFNIISGTSMSCPHLSGIAALIKS 480
Query: 541 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 600
SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI
Sbjct: 481 SHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDI 540
Query: 601 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 660
QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT
Sbjct: 541 QPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPPQTFTRTVT 600
Query: 661 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYL 720
NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKIN+KVT+SVTFKRIGSISPSTEFGKGYL
Sbjct: 601 NVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINEKVTYSVTFKRIGSISPSTEFGKGYL 660
Query: 721 KWVSDKHVVRSPISFKFA 739
KWVSDKHVVRSPISFKFA
Sbjct: 661 KWVSDKHVVRSPISFKFA 678
BLAST of CmoCh02G015390 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 605.9 bits (1561), Expect = 4.3e-173
Identity = 339/730 (46.44%), Postives = 458/730 (62.74%), Query Frame = 0
Query: 31 QTYIV---HVKKPETTDDLEIWHRSFLPSSSSLLYSYRNVMSGFAARL-SEEQVKAMEEM 90
+TYI+ H KPE+ W+ S L S SSLLY+Y GF+A L S E +
Sbjct: 28 KTYIIRVNHSDKPESFLTHHDWYTSQLNSESSLLYTYTTSFHGFSAYLDSTEADSLLSSS 87
Query: 91 DGFVSARRERILQLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDV 150
+ + + + LHTT TP+FLGLN +FG + GVIIGVLD G+ P SFDD
Sbjct: 88 NSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDT 147
Query: 151 GMPPPPPKWKGRCE----FNFSACNNKLIGARSFNLATKALKGETMMDD----SPIDEDG 210
MP P KWKG CE F+ CN KLIGARSF+ + G SP D DG
Sbjct: 148 DMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDG 207
Query: 211 HGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVCFGEDCPDTDILAALDAAI 270
HGTHT++TAAG+ V+ A LG A GTA GMA A +A YKVC+ C +DILAA+D AI
Sbjct: 208 HGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAMDRAI 267
Query: 271 EDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAANSGPFKATLSNEAPWILT 330
DGVDVLSLSLG S P+++D +AIGAF+A+++G+FVSCSA NSGP +A+++N APW++T
Sbjct: 268 LDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMT 327
Query: 331 VAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVYAGEKNQTAALCGEGSLKD 390
V A T+DR A A LGNG+ G SL+ L LVY + ++ LC GSL
Sbjct: 328 VGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDS 387
Query: 391 IDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDGFSTEADAHVLPASHVSHK 450
V+GK+VVC+RG AR+ KG V++AGG MI+ N G AD+H+LPA V K
Sbjct: 388 SIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKK 447
Query: 451 AALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRGPSVASPGILKPDITGPGV 510
++ Y+ S + PTA ++FKGTV+ D SP +A+FSSRGP+ +P ILKPD+ GPGV
Sbjct: 448 TGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGV 507
Query: 511 SILAAW-----PFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKSSHPDWSPAAIKSA 570
+ILA W P LDK+ + ++ FNI+SGTSMSCPH+SG+A L+K++HP+WSP+AIKSA
Sbjct: 508 NILAGWSDAIGPTGLDKD-SRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSA 567
Query: 571 IMTTA---DITNLEGQPIVDENLQPADLFATGAGHVNPSKAADPGLVYDIQPDDYIPYLC 630
+MTTA D TN D +L ++ +A G+GHV+P KA PGLVYDI ++YI +LC
Sbjct: 568 LMTTAYVLDNTNAPLHDAADNSL--SNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLC 627
Query: 631 GLGYKSNEVATIARKP-INCLAKPSIPEGDLNYPSFTVVLGPPQT--FTRTVTNVGCGRE 690
L Y + + I ++P +NC K S P G LNYPSF+V+ G + +TR VTNVG
Sbjct: 628 SLDYTVDHIVAIVKRPSVNCSKKFSDP-GQLNYPSFSVLFGGKRVVRYTREVTNVGAASS 687
Query: 691 VYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPSTEFGKGYLKWVSDKH 738
VY V PS+ ++++PSK+ F + +K ++VTF +S + + G + W + +H
Sbjct: 688 VYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQH 747
BLAST of CmoCh02G015390 vs. TAIR 10
Match:
AT3G14067.1 (Subtilase family protein )
HSP 1 Score: 590.1 bits (1520), Expect = 2.4e-168
Identity = 344/747 (46.05%), Postives = 458/747 (61.31%), Query Frame = 0
Query: 30 LQTYIVHVK---KPETTDDLEIWHRSFL------PSSSSLLYSYRNVMSGFAARLSEEQV 89
L++YIVHV+ KP WH S L P ++LLYSY + GF+ARLS Q
Sbjct: 30 LESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSYSRAVHGFSARLSPIQT 89
Query: 90 KAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPSH 149
A+ +S ++ ++HTTHTP FLG ++ G W +SN+G+ VI+GVLD GI P H
Sbjct: 90 AALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPEH 149
Query: 150 PSFDDVGMPPPPPKWKGRCE----FNFSACNNKLIGARSFNLATKALKGETMM-----DD 209
PSF D G+ P P WKG CE F S+CN KLIGAR+F + T
Sbjct: 150 PSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESR 209
Query: 210 SPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVCFGEDCPDTDIL 269
SP D +GHGTHTASTAAG+ V A A+GTA GMA A +A YK+C+ C D+DIL
Sbjct: 210 SPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICWTGGCYDSDIL 269
Query: 270 AALDAAIEDGVDVLSLSLG-SPSVP-FFQDLVAIGAFAAIQKGIFVSCSAANSGPFKATL 329
AA+D A+ DGV V+SLS+G S S P + D +AIGAF A + GI VSCSA NSGP T
Sbjct: 270 AAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETA 329
Query: 330 SNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPTLLPLVYAGEKNQTAA 389
+N APWILTV AST+DR A A G+G+ F G SL+ P + L LVY+G+ +
Sbjct: 330 TNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVYSGDCG--SR 389
Query: 390 LCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDGFSTEADAHV 449
LC G L V+GK+V+C+RGG AR+ KG+ VK AGGA MIL N + G AD+H+
Sbjct: 390 LCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAESGEELTADSHL 449
Query: 450 LPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASFSSRGPSVASPGIL 509
+PA+ V KA +I+ YI ++ PTA I F GT+IG SP +A+FSSRGP+ +P IL
Sbjct: 450 VPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVIL 509
Query: 510 KPDITGPGVSILAAW-----PFPLDKNRNTKSTFNIISGTSMSCPHLSGIAALIKSSHPD 569
KPD+ PGV+ILA W P LD + + FNIISGTSMSCPH+SG+AAL++ +HPD
Sbjct: 510 KPDVIAPGVNILAGWTGMVGPTDLDIDPR-RVQFNIISGTSMSCPHVSGLAALLRKAHPD 569
Query: 570 WSPAAIKSAIMTTADITNLEGQPIVD-ENLQPADLFATGAGHVNPSKAADPGLVYDIQPD 629
WSPAAIKSA++TTA G+PI D + ++ F GAGHV+P+KA +PGLVYDI+
Sbjct: 570 WSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVK 629
Query: 630 DYIPYLCGLGYKSNEVATIARKPI---NCLAKPSIPEGDLNYPSFTVVL---GPPQTFTR 689
+Y+ +LC +GY+ + + P C GDLNYPSF+VV G + R
Sbjct: 630 EYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKYKR 689
Query: 690 TVTNVGCGRE-VYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKR------IGSIS 738
V NVG + VY V++P ++ + + PSK+ FSK + + VTFK +GS+
Sbjct: 690 VVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSV- 749
BLAST of CmoCh02G015390 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 582.4 bits (1500), Expect = 5.1e-166
Identity = 339/756 (44.84%), Postives = 464/756 (61.38%), Query Frame = 0
Query: 6 SLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEI---WHRSFLPS---SSS 65
+ FLLL L F +V + ++ TYIVH+ K + ++ W+ S L S S+
Sbjct: 10 AFFLLLCLGFCHVSSSSSD-----QGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE 69
Query: 66 LLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNRQFG-FW 125
LLY+Y N + GF+ RL++E+ ++ G +S E +LHTT TP FLGL+ +
Sbjct: 70 LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLF 129
Query: 126 KDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCE----FNFSACNNKLIGAR 185
++ V++GVLD G+ P S+ D G P P WKG CE F S CN KLIGAR
Sbjct: 130 PEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGAR 189
Query: 186 SFNLATKALKG---ETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPL 245
F ++ G E+ SP D+DGHGTHT+STAAG+ V+GA LG A GTA GMAP
Sbjct: 190 FFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPR 249
Query: 246 AHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQK 305
A +A+YKVC+ C +DILAA+D AI D V+VLS+SLG +++D VAIGAFAA+++
Sbjct: 250 ARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMER 309
Query: 306 GIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDF 365
GI VSCSA N+GP ++LSN APWI TV A T+DR A A LGNG+ F G SLF+
Sbjct: 310 GILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL 369
Query: 366 PPTLLPLVYAGEKNQ--TAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGA 425
P LLP +YAG + LC G+L VKGK+V+C+RG AR+ KG VK AGG
Sbjct: 370 PDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGGV 429
Query: 426 AMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNF 485
MIL N +G ADAH+LPA+ V KA I+ Y+ + PTA+I GTV+G
Sbjct: 430 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP- 489
Query: 486 SPAIASFSSRGPSVASPGILKPDITGPGVSILAAW-----PFPLDKNRNTKSTFNIISGT 545
SP +A+FSSRGP+ +P ILKPD+ PGV+ILAAW P L + + + FNIISGT
Sbjct: 490 SPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASD-SRRVEFNIISGT 549
Query: 546 SMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVD-ENLQPADLFATGA 605
SMSCPH+SG+AAL+KS HP+WSPAAI+SA+MTTA T +G+P++D +P+ F GA
Sbjct: 550 SMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGA 609
Query: 606 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYP 665
GHV+P+ A +PGL+YD+ +DY+ +LC L Y S ++ +++R+ C S DLNYP
Sbjct: 610 GHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYP 669
Query: 666 SFTVVLG--PPQTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSV 725
SF V + +TRTVT+VG V + +++ P+ + F + N+K +++V
Sbjct: 670 SFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTV 729
Query: 726 TFKRIGSISPSTEFGKGYLKWVSDKHVVRSPISFKF 738
TF + S PS G ++W KHVV SP++ +
Sbjct: 730 TF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756
BLAST of CmoCh02G015390 vs. TAIR 10
Match:
AT1G04110.1 (Subtilase family protein )
HSP 1 Score: 579.3 bits (1492), Expect = 4.3e-165
Identity = 334/756 (44.18%), Postives = 459/756 (60.71%), Query Frame = 0
Query: 27 LINLQTYIVHV----KKPETTDDLEIWHRSFLPS------------SSSLLYSYRNVMSG 86
++ QTYIV + + +T WH SFL SS LLYSY + + G
Sbjct: 22 ILQKQTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEG 81
Query: 87 FAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLN--RQFGFWKDSNFGKGVI 146
FAA+L+E + + + V+ R + +LQ+ TT++ FLGL+ G W S FG+G I
Sbjct: 82 FAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTI 141
Query: 147 IGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCE----FNFSACNNKLIGARSF----NLAT 206
IGVLD G+ P PSFDD GMP P KWKG C+ F+ S+CN KLIGAR F +A
Sbjct: 142 IGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFFIRGHRVAN 201
Query: 207 KALKGETMMDD--SPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYK 266
+ M + S D GHGTHTAST G+ V A LGN G A GMAP AH+A+YK
Sbjct: 202 SPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYK 261
Query: 267 VCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCS 326
VC+ C +DILAA+D AI+D VDVLSLSLG +P + D +AIG F A+++GI V C+
Sbjct: 262 VCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICA 321
Query: 327 AANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFPPT--LL 386
A N+GP +++++N APW+ T+ A T+DRR A +L NG+ GESL+ +
Sbjct: 322 AGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREV 381
Query: 387 PLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQ 446
++Y ++ + C GSL +++GK+V+C+RG R KG VK AGG AMIL N
Sbjct: 382 EVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMILANT 441
Query: 447 QQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPAIASF 506
+ + D H+LPA+ + + ++ +KAY+N+T P A I+F GTVIG +P +A F
Sbjct: 442 EINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGRSR-APEVAQF 501
Query: 507 SSRGPSVASPGILKPDITGPGVSILAAWP-------FPLDKNRNTKSTFNIISGTSMSCP 566
S+RGPS+A+P ILKPD+ PGV+I+AAWP P D R F ++SGTSMSCP
Sbjct: 502 SARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRR---VNFTVMSGTSMSCP 561
Query: 567 HLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPS 626
H+SGI ALI+S++P+WSPAAIKSA+MTTAD+ + +G+ I D N +PA +FA GAGHVNP
Sbjct: 562 HVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQ 621
Query: 627 KAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINC---LAKPSIPEGDLNYPSFT 686
KA +PGLVY+IQP DYI YLC LG+ +++ I K ++C L K P LNYPS
Sbjct: 622 KAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKN--PGFSLNYPSIA 681
Query: 687 VVLGPPQT---FTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTF 735
V+ +T TR VTNVG +Y+ V+AP I V + P ++ F ++Q +++ V F
Sbjct: 682 VIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWF 741
BLAST of CmoCh02G015390 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 572.4 bits (1474), Expect = 5.3e-163
Identity = 337/746 (45.17%), Postives = 453/746 (60.72%), Query Frame = 0
Query: 29 NLQTYIVHV---KKPETTDDLEIWHRSFLPSSS----SLLYSYRNVMSGFAARLSEEQVK 88
N TYIVHV KP W+ S L S + S++++Y V GF+ARL+ +
Sbjct: 24 NSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARLTSQDAS 83
Query: 89 AMEEMDGFVSARRERILQLHTTHTPDFLGLNR--QFGFWKDSNFGKGVIIGVLDGGIAPS 148
+ + +S E++ LHTT +P+FLGL + G ++S+FG ++IGV+D G+ P
Sbjct: 84 QLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPE 143
Query: 149 HPSFDDVGMPPPPPKWKGRC----EFNFSACNNKLIGARSF---NLATKALKGETMMDDS 208
PSFDD G+ P P KWKG+C +F SACN KL+GAR F AT ET S
Sbjct: 144 RPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRS 203
Query: 209 PIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLAHLAIYKVCFGEDCPDTDILA 268
P D DGHGTHTAS +AG +V A LG A G A GMAP A LA YKVC+ C D+DILA
Sbjct: 204 PRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILA 263
Query: 269 ALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKGIFVSCSAANSGPFKATLSNE 328
A D A+ DGVDV+SLS+G VP++ D +AIGAF AI +GIFVS SA N GP T++N
Sbjct: 264 AFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNV 323
Query: 329 APWILTVAASTIDRRIKAAAKLGNGEEFDGESLF-QPSDFPPTLLPLVYAGE----KNQT 388
APW+ TV A TIDR A KLGNG+ G S++ P P + PLVY G +
Sbjct: 324 APWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYS 383
Query: 389 AALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMILLNQQQDGFSTEADA 448
++LC EGSL VKGK+V+C+RG +R KG V+ GG MI+ N DG AD
Sbjct: 384 SSLCLEGSLDPNLVKGKIVLCDRGIN-SRATKGEIVRKNGGLGMIIANGVFDGEGLVADC 443
Query: 449 HVLPASHVSHKAALKIKAYIN------STTYPTATILFKGTVIGDDNFSPAIASFSSRGP 508
HVLPA+ V +I+ YI+ S+ +PTATI+FKGT +G +P +ASFS+RGP
Sbjct: 444 HVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLG-IRPAPVVASFSARGP 503
Query: 509 SVASPGILKPDITGPGVSILAAWPFPLD----KNRNTKSTFNIISGTSMSCPHLSGIAAL 568
+ +P ILKPD+ PG++ILAAWP + + N ++ FNI+SGTSM+CPH+SG+AAL
Sbjct: 504 NPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAAL 563
Query: 569 IKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENL-QPADLFATGAGHVNPSKAADPGL 628
+K++HPDWSPAAI+SA++TTA + G+P++DE+ + + G+GHV+P+KA DPGL
Sbjct: 564 LKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPGL 623
Query: 629 VYDIQPDDYIPYLCGLGYKSNEVATIARKPINC-LAKPSIPEGDLNYPSFTVVLGP---- 688
VYDI DYI +LC Y + TI R+ +C A+ + G+LNYPSF+VV
Sbjct: 624 VYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGES 683
Query: 689 --PQTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIG-S 734
F RTVTNVG VY + P +VT+ P K+ F ++ QK++F V K
Sbjct: 684 KMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVK 743
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| A9QY38 | 3.9e-280 | 65.34 | Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1 | [more] |
| A9QY40 | 5.2e-248 | 61.93 | Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1 | [more] |
| A0A0M3R8G2 | 6.8e-248 | 59.97 | Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1 | [more] |
| A9QY39 | 6.3e-238 | 59.72 | Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1 | [more] |
| G7KEU7 | 2.9e-235 | 59.62 | Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1H242 | 0.0e+00 | 100.00 | subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111459745 PE... | [more] |
| A0A5A7TJ55 | 0.0e+00 | 83.62 | Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |
| A0A1S3ATZ7 | 0.0e+00 | 83.36 | subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482686 PE=3 SV=... | [more] |
| A0A0A0KKE3 | 0.0e+00 | 82.42 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157240 PE=3 SV=1 | [more] |
| A0A6J1E0E3 | 0.0e+00 | 82.71 | subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111025997 P... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_022958542.1 | 0.0e+00 | 100.00 | subtilisin-like protease SBT1.8 [Cucurbita moschata] | [more] |
| KAG7036083.1 | 0.0e+00 | 99.86 | Subtilisin-like protease SBT1.8, partial [Cucurbita argyrosperma subsp. argyrosp... | [more] |
| KAG6606138.1 | 0.0e+00 | 99.46 | Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| XP_023532974.1 | 0.0e+00 | 98.78 | subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo] | [more] |
| XP_023522248.1 | 0.0e+00 | 98.97 | subtilisin-like protease SBT1.8, partial [Cucurbita pepo subsp. pepo] | [more] |
