CmaCh03G003940.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh03G003940.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Proteasome inhibitor PI31 subunit
Location	Cma_Chr03: 4396781 .. 4401834 (-)
Sequence length	1580
RNA-Seq Expression	CmaCh03G003940.1
Synteny	CmaCh03G003940.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCCATTTCTATTTCTTCTCTTCATCTTCAGCAATGCTCTTCTCGGTTCTGTCGCCGTCGGAGCCGCTCCACCACCGACCGTGGCCGAACCACCTTCCGGCAGAAAGCTCGGGAAGCACCACAGTACGGAGGTTGTTTTTTCGAGTCCGTCTGAAGCACCGCGAAGTGAAAAGAAAGTTCAGAGGATCTCGACGGCGAACGACGGTGGTACGGGGAATGAGATTGAATTGGAAAATCGTGAGCATCATAAGTCGATAGATAAGTCTGTCGCCGGCGGTGGCGTGATATTGGGCGGACTCGCCACCACGTTTTTGGTGGCGATTGTATGTTACATTAGAGCTACGAGGCGACAGAGTGAAGTCAGCCTCTGAGACGACATGTCGTACGAGTTGTTTGTTGGTAATTGCATTTTTTATTATTTTTTTTAAAGGAGGTTGCAATTGTAATTAATCATAAAAATATAAAAGTGAAAGGATTAAATATAATGTATTGTTCTCTTTATTTTTGGTGGAATTTGATTGGTGAATTGCACCAAATAAAACAGTATTGGAAATTGCCATTTAAGTGTTTACTTGTTTTACTAAAAAAAAATAAATTACTTCGTTAATTTAAATTAAATTATTATCCGTTAAATCCCTAATTTAGTCTTCAAAATTCAATTTAATGTCTAATTATACATTTAATTTTCAATTTTGGACCCTGGAAATTTTGTAATTCTTTTTAAAAATATCATTTGGAGAACAATTAAAATTTACACTTTTAAATATATTATATAACCTTTTAAAAATTTTAAGACTTTATTCATTCTTTTATTTTGGTTCCATTGTAATATTTATTTTTAAGATATTATAATTTTGATTTCTAAATTGTCAGAAAATTTTATTTTAACTCTAAATGTTAAAAATTTTCATTTTGATTTTTGCTATATTATTTTGTGAGTTACATTTGGTTTATTGTATTGTTTTATTTTATTTTCTTCAATAATTTTAATGACATGTTTCCAAGATATTTTAAAATATTTATTTTCTTGCACGAAAGTCTCTCTAAACACATACGTTTAAACTTTCAAAATCAATTTTGACTAAATGTAAAATCAAACAATATTTTGATTTTAAATTAATAAATATATGTTTATAGTAATTTTTTAACATAAAAAAAATGTTAGTTCAAAATTTTCAAAATTACTTCTATTTCGTGTTAAAAATATTATATATTTTTAATCATCATAAAAGCAATGGGGATGTAGCTCAGATGGTAGAGCGCTCGCTTAGCATGCGAGAGGTACGGGGATCGATACCCCGCATCTCCAATTTTGTTAAAATTTTAATTTTTAGTATTAAATAATATTTATACTATTTCCGATTACTTCACAACATGTGGGCGTGCCGGAGTGGTTATCGGGCATGACTAGAAATCATGTGGGCTCTGCCCGCGCAGGTTCGAATCCTGCCGCTCACGGTACTGTTTTTTCATTTTTATTTTGTTTTGTTTTCTTGATTATTCCTCGTTCTTTTTTTTTGTTTTATTATTCCTCGTTCTTTATTTATTTATTTTATTATTTCTCGTTCTTTTTTTGTTTTAATATTCCTCGTTCTTTTTTTTATTTTAATATTCCTCGTTCTTTTTTTAATATAAGGGGATGTAACTCAAATGGTAAAGCGATCACTTAGCATACGAGAGACATGGGGATCGATACCCTGCATTTCTATTTTAAATAGACCAAAGACTAGAAAAAATAAATCCTAAATGAATTATTACACATTTTATTCTATAAATTTAAATTAATCTCAAAATATTTAATTTGGTATCTTTGAAGATTGTAATGATATGTTAAATAAAAATATTATAAAAAAGAAACTATGAGCGACCATTTAAAATTTACTTAAAATTAACAAGTCATTAAAAAGTTTTCTTGCTACGAAAGTCATTCATTAGTAGACGCCCCTTGCATATAGAGAGCCACCAAAACTTGCTCTCTCTGGCTCTCCGTCTGGTTCGTCGGAATTGAATTGGAGAAGTACAAAGAATCTGCAAGGCAATACAAAAATGGTGAATGAGAAATCGGTTATGGCCGTCATCAGGGCAACTAGGCCTACCTTTCGCAATGACAACGACAAGGTCGCGTTTGCGGTTCACGCCATATTTCTCGCCTCCGGCTGCGTGCTCACTGCCACCGGACCTCCTGCTTTTACTGACGCCGCCTTTTCTTCTCCCTCAAAGGGTATGACTTCTTTGTTTATTGAAAAATTAAAAATTCTTCCCATATTTGGTATTTCTGAGTCTTGGATTGGGGTTTAGATGAAATTAGTATCGAGGGCTGGAACGAATTAGAGGATGAGTACGCATACGTTTACGCAAACCTTGAAAAGGATTCTAACTGGAAGAAAGTGATAGTGAAGTGCCTGGTGATGAACGGGAAATTGCTTATTGATGCCTTGACTGATGAGAATTCCGAGCCACTCCATCTTGAAATTGAGTACGCTCTCCCAGATATCAGATATATATGTGTATTGGCGAATTAGTACTTTGTCCTGTTTCGGTATTGAGTGTTTTTTTGTTCCTATTGTTCTTTTAGCGTTGAAGAAATCGTCAATCCGAATGGGGGAAGTAACTATTCAACGCAGTACAGAAATTTGGATACCCTGGTGAAACGCTTGGGCTTGGGAATATTGTCAAAATTGGGTGGTTCTTCGAAAGCCACGACAAGTAATCCAGCAAGGTAGTGATTAGATCAGTAATCAACACGTACGGTTATTTTAATTCTTCATTTTTCCTGATGTTATTGAGACGATTACTCTCTTTTGTGATTCTTATTTCTTGGCGTGTTATAAATATGAATCGAAATTATGTCTCTGGTAATCGTTCTTTAATCTGGTTCTTGGCGGTCTACATGCTTTTTGCAGGGCTGAGAGTGAGAGTAGTGAAAGATCATCTGTAGTTGTAAATAAGCCTACTTCTGGAATTCCTGAGCTTCCTGGTCCACTGCTCGATCCTTCCGGGTCTTTCTCTTTATCCACACTTGCGCAATCACAAAACGTCATGACATAATTTGAGAAATATGTTGGGTCTTGACTTAAGTTCTTAACTATTGTATGCTACCATGCTTTTACGTTTGTGTTGGGTGCTATTATCACTGTCTCCAACATTCAGATTTGCTAGTTTTTAAGTTTCTAACAGTAATTTGAATGCATTTTCTTTATGATATCTTGTTTTCCCCTTTATGGTTAGATCAAGAAGATAATGGTTTATATGCTTTATGCTTTGATTGATTTGATACCCAGTTCCTCTTAGGTTTGATTGATTTGACACAAGAAGATAATGGTTTCCTTTGAGAGAGATTGTAATAGTCTTACTTAATGTGCAGGAATTGTTTTTGCTGAATTATTGCGACGGTCAAATATTTAATGGCAAGCATATTGTGCCTTTGTCCAATCCTTAATTGTATCAATTTAGGAATTTTCGAAGGTTCCATGTTTACAAATAATTTAGCTAAGAACCTGTATCCCATGAGTGAGTGAGTGAGTTGACATAGTCTGAATGTGGCTGTTTTTGCTATTCTCTTAGAGTTGTAATTCCTCCTATTAATCCCCTTGGTGGTGGCGATCTTTTCCCTGGACCCGGTGCTGAAATGTACCCTGCTGGGTAATTTTTTAAGTTTGTCGATTAATTGTGTACGTATGTGTAACAGTCCAAGCTTACCGTTAGTAGATATTGTTTTTTTTGGGCATTCTCTCAAGGTTTTTAAAACGTGTTTGCTAGTGAGAGGTCTCCACAATCTTAAAAAGAATGTTTGTTCCCCTCTCCAATCGATGTGGGATCTCACAATCCAATCCCTTCGAGGCTCAGCATTCTCGTTGGCACTTTATACCCTCTCCAAGGGGAACAAAACATTTATTATAAAGGTGTGGAAACTTGTCCCTAGCAGACGTGTTTTAAAAACCTTGAGAGAAAACTCGAAAAGAAAAACACAACGAGAACAATATCTGCCAGTGATGGACTTGAGAGCTGTTACAATATGATTGTCATTGGTGCAAAATATCATGTTAATTTTGTTTTTATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAATTGATTACAGAGGTGGTTTCAGCGGCGATGGAAGCATGCTTTTAGGTTAGTGAATTGTTAAGTCATCTAGTATTAGATTGAAAATAAGCGTAATTTCACTATTTTATCATGCAGGACCGAATGACCCGCGATGGTTAGGAATTGGTATAGGACCTGGATTTGAAGGCGGGCAGCCGTGAGTCAATTAGCTCTCTCTCTCTCTCACTCATAAAGACACACACAAACACTCACCTTTGCATTATGACTGCTGCTAATTCCAAGTATCTTGAAACAGGGGTGTTCCTCCAGGTGCTCGTTTTGATCCATATGGTCCACCTGGTGTTCCTGGTTTTGAACCCTTTCGGTTTGTTAGGTATGTGGAACTTTCCTTACCTTGTCATTGCATATGGGGGGCTCTCTTTTGATCATTAATTTCTATCAGCATTTATCATTTGCTGAATGCTGCTTTGACAACGACTAAATTCAAACCATGTATCTTGGCATTCTAAAATAGGATTGGTTAATCCATTGAGAAACAGGATTGGTTGGGTCATTAGCCCAGTTGTCATTCATACAAACAACTTGTTTCTGAATACATTTTGTATGGAGCGGCAGAGTAGTTAGTTAACTGGGATGCTTCTTCTTCTTCTTCTTCTTCCCTTTGTTACAGGAATCCTGGGAGACCTGGAGGGGGAACTCATCCGGACCTGGAGCATTTTGGAGACGGTTCAGATTTCATATAGTCCGCCATAGTTATAAAGCTCTGAAAGTTGTTCAAAGTGTTAGGGAAAGACGATAAATTTGGGGGATGGATGGTCAACCCATTTTATGCATATGCTTGTATTGAGTTCCTTTTTTCGTATGTTTTAGGTGGCCAGTAATGACTGTATGTACGGTGAATATAGGAATGGTGTTTACTGAATTTTACGTGTCAAAAAACATTTTAACTTCAAACTTTTACACCCATATA

mRNA sequence

ATGGCTCCATTTCTATTTCTTCTCTTCATCTTCAGCAATGCTCTTCTCGGTTCTGTCGCCGTCGGAGCCGCTCCACCACCGACCGTGGCCGAACCACCTTCCGGCAGAAAGCTCGGGAAGCACCACAGTACGGAGGTTGTTTTTTCGAGTCCGTCTGAAGCACCGCGAAGTGAAAAGAAAGTTCAGAGGATCTCGACGGCGAACGACGGTGGTACGGGGAATGAGATTGAATTGGAAAATCGTGAGCATCATAAGTCGATAGATAAGTCTGTCGCCGGCGGTGGCGTGATATTGGGCGGACTCGCCACCACGTTTTTGGTGGCGATTACGCCCCTTGCATATAGAGAGCCACCAAAACTTGCTCTCTCTGGCTCTCCGTCTGGTTCGTCGGAATTGAATTGGAGAAGTACAAAGAATCTGCAAGGCAATACAAAAATGGTGAATGAGAAATCGGTTATGGCCGTCATCAGGGCAACTAGGCCTACCTTTCGCAATGACAACGACAAGGTCGCGTTTGCGGTTCACGCCATATTTCTCGCCTCCGGCTGCGTGCTCACTGCCACCGGACCTCCTGCTTTTACTGACGCCGCCTTTTCTTCTCCCTCAAAGGATGAAATTAGTATCGAGGGCTGGAACGAATTAGAGGATGAGTACGCATACGTTTACGCAAACCTTGAAAAGGATTCTAACTGGAAGAAAGTGATAGTGAAGTGCCTGGTGATGAACGGGAAATTGCTTATTGATGCCTTGACTGATGAGAATTCCGAGCCACTCCATCTTGAAATTGACGTTGAAGAAATCGTCAATCCGAATGGGGGAAGTAACTATTCAACGCAGTACAGAAATTTGGATACCCTGGTGAAACGCTTGGGCTTGGGAATATTGTCAAAATTGGGTGGTTCTTCGAAAGCCACGACAAGTAATCCAGCAAGGGCTGAGAGTGAGAGTAGTGAAAGATCATCTGTAGTTGTAAATAAGCCTACTTCTGGAATTCCTGAGCTTCCTGGTCCACTGCTCGATCCTTCCGGAGTTGTAATTCCTCCTATTAATCCCCTTGGTGGTGGCGATCTTTTCCCTGGACCCGGTGCTGAAATGTACCCTGCTGGAGGTGGTTTCAGCGGCGATGGAAGCATGCTTTTAGGACCGAATGACCCGCGATGGTTAGGAATTGGTATAGGACCTGGATTTGAAGGCGGGCAGCCGGGTGTTCCTCCAGGTGCTCGTTTTGATCCATATGGTCCACCTGGTGTTCCTGGTTTTGAACCCTTTCGGTTTGTTAGGAATCCTGGGAGACCTGGAGGGGGAACTCATCCGGACCTGGAGCATTTTGGAGACGGTTCAGATTTCATATAGTCCGCCATAGTTATAAAGCTCTGAAAGTTGTTCAAAGTGTTAGGGAAAGACGATAAATTTGGGGGATGGATGGTCAACCCATTTTATGCATATGCTTGTATTGAGTTCCTTTTTTCGTATGTTTTAGGTGGCCAGTAATGACTGTATGTACGGTGAATATAGGAATGGTGTTTACTGAATTTTACGTGTCAAAAAACATTTTAACTTCAAACTTTTACACCCATATA

Coding sequence (CDS)

ATGGCTCCATTTCTATTTCTTCTCTTCATCTTCAGCAATGCTCTTCTCGGTTCTGTCGCCGTCGGAGCCGCTCCACCACCGACCGTGGCCGAACCACCTTCCGGCAGAAAGCTCGGGAAGCACCACAGTACGGAGGTTGTTTTTTCGAGTCCGTCTGAAGCACCGCGAAGTGAAAAGAAAGTTCAGAGGATCTCGACGGCGAACGACGGTGGTACGGGGAATGAGATTGAATTGGAAAATCGTGAGCATCATAAGTCGATAGATAAGTCTGTCGCCGGCGGTGGCGTGATATTGGGCGGACTCGCCACCACGTTTTTGGTGGCGATTACGCCCCTTGCATATAGAGAGCCACCAAAACTTGCTCTCTCTGGCTCTCCGTCTGGTTCGTCGGAATTGAATTGGAGAAGTACAAAGAATCTGCAAGGCAATACAAAAATGGTGAATGAGAAATCGGTTATGGCCGTCATCAGGGCAACTAGGCCTACCTTTCGCAATGACAACGACAAGGTCGCGTTTGCGGTTCACGCCATATTTCTCGCCTCCGGCTGCGTGCTCACTGCCACCGGACCTCCTGCTTTTACTGACGCCGCCTTTTCTTCTCCCTCAAAGGATGAAATTAGTATCGAGGGCTGGAACGAATTAGAGGATGAGTACGCATACGTTTACGCAAACCTTGAAAAGGATTCTAACTGGAAGAAAGTGATAGTGAAGTGCCTGGTGATGAACGGGAAATTGCTTATTGATGCCTTGACTGATGAGAATTCCGAGCCACTCCATCTTGAAATTGACGTTGAAGAAATCGTCAATCCGAATGGGGGAAGTAACTATTCAACGCAGTACAGAAATTTGGATACCCTGGTGAAACGCTTGGGCTTGGGAATATTGTCAAAATTGGGTGGTTCTTCGAAAGCCACGACAAGTAATCCAGCAAGGGCTGAGAGTGAGAGTAGTGAAAGATCATCTGTAGTTGTAAATAAGCCTACTTCTGGAATTCCTGAGCTTCCTGGTCCACTGCTCGATCCTTCCGGAGTTGTAATTCCTCCTATTAATCCCCTTGGTGGTGGCGATCTTTTCCCTGGACCCGGTGCTGAAATGTACCCTGCTGGAGGTGGTTTCAGCGGCGATGGAAGCATGCTTTTAGGACCGAATGACCCGCGATGGTTAGGAATTGGTATAGGACCTGGATTTGAAGGCGGGCAGCCGGGTGTTCCTCCAGGTGCTCGTTTTGATCCATATGGTCCACCTGGTGTTCCTGGTTTTGAACCCTTTCGGTTTGTTAGGAATCCTGGGAGACCTGGAGGGGGAACTCATCCGGACCTGGAGCATTTTGGAGACGGTTCAGATTTCATATAG

Protein sequence

MAPFLFLLFIFSNALLGSVAVGAAPPPTVAEPPSGRKLGKHHSTEVVFSSPSEAPRSEKKVQRISTANDGGTGNEIELENREHHKSIDKSVAGGGVILGGLATTFLVAITPLAYREPPKLALSGSPSGSSELNWRSTKNLQGNTKMVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVEEIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLGPNDPRWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGDGSDFI

Homology

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BLAST of CmaCh03G003940.1 vs. ExPASy Swiss-Prot
Match: Q9M330 (Probable proteasome inhibitor OS=Arabidopsis thaliana OX=3702 GN=At3g53970 PE=1 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 7.9e-82
Identity = 175/311 (56.27%), Postives = 212/311 (68.17%), Query Frame = 0


Query: 146 MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 205
           M N ++VMA+IR  RP FRN++DKVAFA+H+ F+ASG +LTATG PAF D A SS S+++
Sbjct: 1   MANSQTVMAMIRLARPPFRNNHDKVAFAIHSSFVASGYILTATGRPAFADEALSSSSQND 60

Query: 206 ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 265
           + IEGWNE E EYA+VYAN +K S  KK++VKCL M+ KLL+DA+ D  +EP HLEI V 
Sbjct: 61  VGIEGWNEFEGEYAFVYANPKKGS--KKILVKCLAMDDKLLVDAIADGGAEPAHLEIKVG 120

Query: 266 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 325
           +    +   +YS Q++NLD LV  L   I+ KL G  K   S  A++ SE++E      +
Sbjct: 121 DYAEESNEGDYSAQFKNLDKLVTDLQSEIIDKLDGKPKPVASR-AQSSSETNEEPRYYDD 180

Query: 326 KPTSGIPELPGPLLDPSGVVIPPINPLGG-GDLFPGPGAEMYPAGGGFSGDGSMLLGPND 385
            P        GP + PSGVV+PPI   GG  DLFPGPGA MYP  GGF GDGSML+GP D
Sbjct: 181 TPNP-----LGPQIHPSGVVVPPIPGNGGYSDLFPGPGAGMYPGRGGF-GDGSMLVGPTD 240

Query: 386 PRWLGIGIG---PGFEG-GQPGV-PPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPD 445
           PR+   G G   PGF G   PG+ PPGARFDPYGPPGVPGFEP RF R P R  GG HPD
Sbjct: 241 PRFFPFGDGSDRPGFMGPPHPGMPPPGARFDPYGPPGVPGFEPGRFTRQPPRGPGGGHPD 300

Query: 446 LEHFGDGSDFI 451
           LEHF  GSDFI
Sbjct: 301 LEHFPGGSDFI 302

[Close]

BLAST of CmaCh03G003940.1 vs. ExPASy Swiss-Prot
Match: Q92530 (Proteasome inhibitor PI31 subunit OS=Homo sapiens OX=9606 GN=PSMF1 PE=1 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 2.1e-05
Identity = 65/232 (28.02%), Postives = 104/232 (44.83%), Query Frame = 0


Query: 203 KDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEI 262
           K E+   GWN  +D Y   Y   E     +K++VK + +   ++++ L   + +   L +
Sbjct: 50  KSELLPAGWNNNKDLYVLRY---EYKDGSRKLLVKAITVESSMILNVLEYGSQQVADLTL 109

Query: 263 DVEEIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGS-SKATTSNPARAESESSERSS 322
           ++++ ++     ++   Y+N + L  R+  GI++ +     KA  S+P R    ++ R  
Sbjct: 110 NLDDYIDAEHLGDFHRTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPPATAREV 169

Query: 323 VVVNKP-----TSGIPELPGPLLDPSGVVIPPINPLGGGDLFP-GP---GAEMYPAGGGF 382
             +  P     TS  P    P  DP G  +     +GG DL P GP   G  + P   GF
Sbjct: 170 DPLRIPPHHPHTSRQP----PWCDPLGPFV-----VGGEDLDPFGPRRGGMIVDPLRSGF 229

Query: 383 SGDGSMLLGPNDPRWLGIGIGPGFEGGQPGVPPGARFDPYGPPGV--PGFEP 423
                 L+ P+    L   + PG       VPPGARFDP+GP G   PG  P
Sbjct: 230 P---RALIDPSS--GLPNRLPPG------AVPPGARFDPFGPIGTSPPGPNP 258

[Close]

BLAST of CmaCh03G003940.1 vs. ExPASy Swiss-Prot
Match: Q5RDN3 (Proteasome inhibitor PI31 subunit OS=Pongo abelii OX=9601 GN=PSMF1 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 3.9e-04
Identity = 62/228 (27.19%), Postives = 100/228 (43.86%), Query Frame = 0


Query: 203 KDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEI 262
           K E+   GWN  +D Y   Y   E     +K++VK + +   ++++ L   + +   L +
Sbjct: 50  KSELLPAGWNNNKDLYVLRY---EYKDGSRKLLVKAITVESSMILNVLEYGSQQVADLTL 109

Query: 263 DVEEIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGS-SKATTSNPARAESESSERSS 322
           ++++ ++     ++   Y+N + L  R+  GI++ +     KA  S+P R    ++ R  
Sbjct: 110 NLDDYIDAEHLGDFHRTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPPATAREV 169

Query: 323 VVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLG 382
             +  P    P  P     P     P  +PLG    F   G ++ P G      G M++ 
Sbjct: 170 DPLRIP----PHHPHTSRQP-----PWCDPLGP---FAVGGEDLDPFG---HRRGGMIVD 229

Query: 383 P---NDPRWLGIGIGPGFEGGQP--GVPPGARFDPYGPPGV--PGFEP 423
           P     PR L I    G     P   VPPGARFDP+GP G   PG  P
Sbjct: 230 PLRSGFPRAL-IDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPPGPNP 258

[Close]

BLAST of CmaCh03G003940.1 vs. ExPASy TrEMBL
Match: A0A6J1GF71 (probable proteasome inhibitor isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453381 PE=4 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 1.2e-173
Identity = 306/319 (95.92%), Postives = 310/319 (97.18%), Query Frame = 0


Query: 132 LNWRSTKNLQGNTKMVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 191
           +NWRSTKNLQ NTKMVNE+SVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP
Sbjct: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60

Query: 192 AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 251
           AFTDAAFSSPSKDEISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALT
Sbjct: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALT 120

Query: 252 DENSEPLHLEIDVEEIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPAR 311
           DENSEPLHLEIDVE+IVNPNGGSNYSTQYRNLDTLVKRLG GILSKLGGSSKATTSNPAR
Sbjct: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180

Query: 312 AESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGG 371
           AESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGA MYP GGG
Sbjct: 181 AESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAGMYPTGGG 240

Query: 372 FSGDGSMLLGPNDPRWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGR 431
           F GDGSMLLGPNDPRWLG G GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRF RNPGR
Sbjct: 241 FGGDGSMLLGPNDPRWLGTGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARNPGR 300

Query: 432 PGGGTHPDLEHFGDGSDFI 451
           PGGGTHPDLEHFGDGSDFI
Sbjct: 301 PGGGTHPDLEHFGDGSDFI 319

[Close]

BLAST of CmaCh03G003940.1 vs. ExPASy TrEMBL
Match: A0A6J1ISG6 (probable proteasome inhibitor isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478282 PE=4 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 6.5e-172
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0


Query: 146 MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 205
           MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 1   MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60

Query: 206 ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 265
           ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120

Query: 266 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 325
           EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN
Sbjct: 121 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 180

Query: 326 KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLGPNDP 385
           KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLGPNDP
Sbjct: 181 KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLGPNDP 240

Query: 386 RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 445
           RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD
Sbjct: 241 RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 300

Query: 446 GSDFI 451
           GSDFI
Sbjct: 301 GSDFI 305

[Close]

BLAST of CmaCh03G003940.1 vs. ExPASy TrEMBL
Match: A0A6J1GF52 (probable proteasome inhibitor isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453381 PE=4 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 9.5e-155
Identity = 282/319 (88.40%), Postives = 286/319 (89.66%), Query Frame = 0


Query: 132 LNWRSTKNLQGNTKMVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 191
           +NWRSTKNLQ NTKMVNE+SVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP
Sbjct: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60

Query: 192 AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 251
           AFTDAAFSSPSKDEISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALT
Sbjct: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALT 120

Query: 252 DENSEPLHLEIDVEEIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPAR 311
           DENSEPLHLEIDVE+IVNPNGGSNYSTQYRNLDTLVKRLG GILSKLGGSSKATTSNPAR
Sbjct: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180

Query: 312 AESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGG 371
           AESESSERSSVVVNKPTSGIPELPGPLLDPS                          GGG
Sbjct: 181 AESESSERSSVVVNKPTSGIPELPGPLLDPS--------------------------GGG 240

Query: 372 FSGDGSMLLGPNDPRWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGR 431
           F GDGSMLLGPNDPRWLG G GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRF RNPGR
Sbjct: 241 FGGDGSMLLGPNDPRWLGTGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARNPGR 293

Query: 432 PGGGTHPDLEHFGDGSDFI 451
           PGGGTHPDLEHFGDGSDFI
Sbjct: 301 PGGGTHPDLEHFGDGSDFI 293

[Close]

BLAST of CmaCh03G003940.1 vs. ExPASy TrEMBL
Match: A0A6J1ITK3 (probable proteasome inhibitor isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478282 PE=4 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 1.3e-151
Identity = 279/305 (91.48%), Postives = 279/305 (91.48%), Query Frame = 0


Query: 146 MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 205
           MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 1   MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60

Query: 206 ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 265
           ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120

Query: 266 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 325
           EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN
Sbjct: 121 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 180

Query: 326 KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLGPNDP 385
           KPTSGIPELPGPLLDPS                          GGGFSGDGSMLLGPNDP
Sbjct: 181 KPTSGIPELPGPLLDPS--------------------------GGGFSGDGSMLLGPNDP 240

Query: 386 RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 445
           RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD
Sbjct: 241 RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 279

Query: 446 GSDFI 451
           GSDFI
Sbjct: 301 GSDFI 279

[Close]

BLAST of CmaCh03G003940.1 vs. ExPASy TrEMBL
Match: A0A6J1GE95 (probable proteasome inhibitor isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111453381 PE=4 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.9e-151
Identity = 274/319 (85.89%), Postives = 278/319 (87.15%), Query Frame = 0


Query: 132 LNWRSTKNLQGNTKMVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 191
           +NWRSTKNLQ NTKMVNE+SVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP
Sbjct: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60

Query: 192 AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 251
           AFTDAAFSSPSKDEISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALT
Sbjct: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALT 120

Query: 252 DENSEPLHLEIDVEEIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPAR 311
           DENSEPLHLEIDVE+IVNPNGGSNYSTQYRNLDTLVKRLG GILSKLGGSSKATTSNPAR
Sbjct: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180

Query: 312 AESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGG 371
                                           VVIPPINPLGGGDLFPGPGA MYP GGG
Sbjct: 181 --------------------------------VVIPPINPLGGGDLFPGPGAGMYPTGGG 240

Query: 372 FSGDGSMLLGPNDPRWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGR 431
           F GDGSMLLGPNDPRWLG G GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRF RNPGR
Sbjct: 241 FGGDGSMLLGPNDPRWLGTGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARNPGR 287

Query: 432 PGGGTHPDLEHFGDGSDFI 451
           PGGGTHPDLEHFGDGSDFI
Sbjct: 301 PGGGTHPDLEHFGDGSDFI 287

[Close]

BLAST of CmaCh03G003940.1 vs. NCBI nr
Match: XP_022950229.1 (probable proteasome inhibitor isoform X1 [Cucurbita moschata])

HSP 1 Score: 619.4 bits (1596), Expect = 2.5e-173
Identity = 306/319 (95.92%), Postives = 310/319 (97.18%), Query Frame = 0


Query: 132 LNWRSTKNLQGNTKMVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 191
           +NWRSTKNLQ NTKMVNE+SVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP
Sbjct: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60

Query: 192 AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 251
           AFTDAAFSSPSKDEISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALT
Sbjct: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALT 120

Query: 252 DENSEPLHLEIDVEEIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPAR 311
           DENSEPLHLEIDVE+IVNPNGGSNYSTQYRNLDTLVKRLG GILSKLGGSSKATTSNPAR
Sbjct: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180

Query: 312 AESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGG 371
           AESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGA MYP GGG
Sbjct: 181 AESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAGMYPTGGG 240

Query: 372 FSGDGSMLLGPNDPRWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGR 431
           F GDGSMLLGPNDPRWLG G GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRF RNPGR
Sbjct: 241 FGGDGSMLLGPNDPRWLGTGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARNPGR 300

Query: 432 PGGGTHPDLEHFGDGSDFI 451
           PGGGTHPDLEHFGDGSDFI
Sbjct: 301 PGGGTHPDLEHFGDGSDFI 319

[Close]

BLAST of CmaCh03G003940.1 vs. NCBI nr
Match: XP_022978238.1 (probable proteasome inhibitor isoform X1 [Cucurbita maxima])

HSP 1 Score: 613.6 bits (1581), Expect = 1.3e-171
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0


Query: 146 MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 205
           MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 1   MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60

Query: 206 ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 265
           ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120

Query: 266 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 325
           EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN
Sbjct: 121 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 180

Query: 326 KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLGPNDP 385
           KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLGPNDP
Sbjct: 181 KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLGPNDP 240

Query: 386 RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 445
           RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD
Sbjct: 241 RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 300

Query: 446 GSDFI 451
           GSDFI
Sbjct: 301 GSDFI 305

[Close]

BLAST of CmaCh03G003940.1 vs. NCBI nr
Match: XP_023545137.1 (probable proteasome inhibitor isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 595.1 bits (1533), Expect = 5.0e-166
Identity = 295/305 (96.72%), Postives = 298/305 (97.70%), Query Frame = 0


Query: 146 MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 205
           MVNE+SVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60

Query: 206 ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 265
           ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120

Query: 266 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 325
           +IVNPNGGSNYSTQYRNLDTLVKRLG GI SKLGG SKATTSNPARAESESSERSSVVVN
Sbjct: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180

Query: 326 KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLGPNDP 385
           KPTSGIPELPGPLLDPSGVVIPPINP GGGDLFPGPGA MYPAGGG SGDGSMLLGPNDP
Sbjct: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGGGDLFPGPGAGMYPAGGGLSGDGSMLLGPNDP 240

Query: 386 RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 445
           RWLG+G GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD
Sbjct: 241 RWLGVGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 300

Query: 446 GSDFI 451
           GSDFI
Sbjct: 301 GSDFI 305

[Close]

BLAST of CmaCh03G003940.1 vs. NCBI nr
Match: KAG6603621.1 (putative proteasome inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 559.3 bits (1440), Expect = 3.0e-155
Identity = 283/298 (94.97%), Postives = 286/298 (95.97%), Query Frame = 0


Query: 132 LNWRSTKNLQGNTKMVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 191
           +NWRSTKNLQ NTKMVNE+SVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP
Sbjct: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60

Query: 192 AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 251
           AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT
Sbjct: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 120

Query: 252 DENSEPLHLEIDVEEIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPAR 311
           DENSEPLHLEIDVE+IVNPNGGSNYSTQYRNLDTLVKRLG GILSKLGGSSKATTSNPAR
Sbjct: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180

Query: 312 AESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGG 371
           AESESSERSSVVVNKP   IPELPGPLLDPSGVVIPPINPLGGGDLFPGP A MYP GGG
Sbjct: 181 AESESSERSSVVVNKP---IPELPGPLLDPSGVVIPPINPLGGGDLFPGPVAGMYPTGGG 240

Query: 372 FSGDGSMLLGPNDPRWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNP 430
           FSGDGSMLLGPNDPRWLG G GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRF R P
Sbjct: 241 FSGDGSMLLGPNDPRWLGTGKGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARWP 295

[Close]

BLAST of CmaCh03G003940.1 vs. NCBI nr
Match: XP_022950230.1 (probable proteasome inhibitor isoform X2 [Cucurbita moschata])

HSP 1 Score: 556.6 bits (1433), Expect = 2.0e-154
Identity = 282/319 (88.40%), Postives = 286/319 (89.66%), Query Frame = 0


Query: 132 LNWRSTKNLQGNTKMVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 191
           +NWRSTKNLQ NTKMVNE+SVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP
Sbjct: 1   MNWRSTKNLQSNTKMVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPP 60

Query: 192 AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALT 251
           AFTDAAFSSPSKDEISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALT
Sbjct: 61  AFTDAAFSSPSKDEISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALT 120

Query: 252 DENSEPLHLEIDVEEIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPAR 311
           DENSEPLHLEIDVE+IVNPNGGSNYSTQYRNLDTLVKRLG GILSKLGGSSKATTSNPAR
Sbjct: 121 DENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR 180

Query: 312 AESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGG 371
           AESESSERSSVVVNKPTSGIPELPGPLLDPS                          GGG
Sbjct: 181 AESESSERSSVVVNKPTSGIPELPGPLLDPS--------------------------GGG 240

Query: 372 FSGDGSMLLGPNDPRWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGR 431
           F GDGSMLLGPNDPRWLG G GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRF RNPGR
Sbjct: 241 FGGDGSMLLGPNDPRWLGTGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARNPGR 293

Query: 432 PGGGTHPDLEHFGDGSDFI 451
           PGGGTHPDLEHFGDGSDFI
Sbjct: 301 PGGGTHPDLEHFGDGSDFI 293

[Close]

BLAST of CmaCh03G003940.1 vs. TAIR 10
Match: AT3G53970.1 (proteasome inhibitor-related )

HSP 1 Score: 305.8 bits (782), Expect = 5.6e-83
Identity = 175/311 (56.27%), Postives = 212/311 (68.17%), Query Frame = 0


Query: 146 MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 205
           M N ++VMA+IR  RP FRN++DKVAFA+H+ F+ASG +LTATG PAF D A SS S+++
Sbjct: 1   MANSQTVMAMIRLARPPFRNNHDKVAFAIHSSFVASGYILTATGRPAFADEALSSSSQND 60

Query: 206 ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 265
           + IEGWNE E EYA+VYAN +K S  KK++VKCL M+ KLL+DA+ D  +EP HLEI V 
Sbjct: 61  VGIEGWNEFEGEYAFVYANPKKGS--KKILVKCLAMDDKLLVDAIADGGAEPAHLEIKVG 120

Query: 266 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 325
           +    +   +YS Q++NLD LV  L   I+ KL G  K   S  A++ SE++E      +
Sbjct: 121 DYAEESNEGDYSAQFKNLDKLVTDLQSEIIDKLDGKPKPVASR-AQSSSETNEEPRYYDD 180

Query: 326 KPTSGIPELPGPLLDPSGVVIPPINPLGG-GDLFPGPGAEMYPAGGGFSGDGSMLLGPND 385
            P        GP + PSGVV+PPI   GG  DLFPGPGA MYP  GGF GDGSML+GP D
Sbjct: 181 TPNP-----LGPQIHPSGVVVPPIPGNGGYSDLFPGPGAGMYPGRGGF-GDGSMLVGPTD 240

Query: 386 PRWLGIGIG---PGFEG-GQPGV-PPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPD 445
           PR+   G G   PGF G   PG+ PPGARFDPYGPPGVPGFEP RF R P R  GG HPD
Sbjct: 241 PRFFPFGDGSDRPGFMGPPHPGMPPPGARFDPYGPPGVPGFEPGRFTRQPPRGPGGGHPD 300

Query: 446 LEHFGDGSDFI 451
           LEHF  GSDFI
Sbjct: 301 LEHFPGGSDFI 302

[Close]

BLAST of CmaCh03G003940.1 vs. TAIR 10
Match: AT3G53970.2 (proteasome inhibitor-related )

HSP 1 Score: 234.6 bits (597), Expect = 1.6e-61
Identity = 135/257 (52.53%), Postives = 171/257 (66.54%), Query Frame = 0


Query: 146 MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 205
           M N ++VMA+IR  RP FRN++DKVAFA+H+ F+ASG +LTATG PAF D A SS S+++
Sbjct: 1   MANSQTVMAMIRLARPPFRNNHDKVAFAIHSSFVASGYILTATGRPAFADEALSSSSQND 60

Query: 206 ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 265
           + IEGWNE E EYA+VYAN +K S  KK++VKCL M+ KLL+DA+ D  +EP HLEI V 
Sbjct: 61  VGIEGWNEFEGEYAFVYANPKKGS--KKILVKCLAMDDKLLVDAIADGGAEPAHLEIKVG 120

Query: 266 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 325
           +    +   +YS Q++NLD LV  L   I+ KL G  K   S  A++ SE++E      +
Sbjct: 121 DYAEESNEGDYSAQFKNLDKLVTDLQSEIIDKLDGKPKPVASR-AQSSSETNEEPRYYDD 180

Query: 326 KPTSGIPELPGPLLDPSGVVIPPINPLGG-GDLFPGPGAEMYPAGGGFSGDGSMLLGPND 385
            P        GP + PSGVV+PPI   GG  DLFPGPGA MYP  GGF GDGSML+GP D
Sbjct: 181 TPNP-----LGPQIHPSGVVVPPIPGNGGYSDLFPGPGAGMYPGRGGF-GDGSMLVGPTD 240

Query: 386 PRWLGIGIG---PGFEG 399
           PR+   G G   PGF G
Sbjct: 241 PRFFPFGDGSDRPGFMG 248

[Close]

BLAST of CmaCh03G003940.1 vs. TAIR 10
Match: AT1G48530.1 (CONTAINS InterPro DOMAIN/s: Proteasome Inhibitor PI31 (InterPro:IPR021625); BEST Arabidopsis thaliana protein match is: proteasome inhibitor-related (TAIR:AT3G53970.2); Has 43 Blast hits to 43 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 11; Fungi - 0; Plants - 32; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 132.1 bits (331), Expect = 1.1e-30
Identity = 75/165 (45.45%), Postives = 101/165 (61.21%), Query Frame = 0


Query: 146 MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFT-DAAFSSPSKD 205
           M    +VM VIR+  P FRN NDK+AF VHA    SG +LT+TG PAF  DA  SS ++ 
Sbjct: 12  MATTHAVMGVIRSVMPAFRNKNDKIAFVVHASLAVSGFILTSTGRPAFAHDALSSSTTQC 71

Query: 206 EISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDV 265
            + IEGWNE ++EYA+VY         K+ +VKCL MN KLL+DA+ ++  E  HL+I+V
Sbjct: 72  LVGIEGWNEFDEEYAFVY-----KCPVKRYLVKCLAMNDKLLVDAIAEDGKEFGHLQIEV 131

Query: 266 EEIVNPNG-GSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSN 309
              ++ +G   +Y TQ++N D LV  L   IL KL   S  T S+
Sbjct: 132 GNYIDESGEEGDYDTQFKNFDKLVTELKSEILCKLNTVSTTTKSS 171

[Close]

BLAST of CmaCh03G003940.1 vs. TAIR 10
Match: AT3G09280.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: root; Has 31 Blast hits to 31 proteins in 9 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 31; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 57.0 bits (136), Expect = 4.5e-08
Identity = 34/80 (42.50%), Postives = 44/80 (55.00%), Query Frame = 0


Query: 30  AEPPSGRKLGKHHSTEVVFSSPSEAPRSEKKVQRISTANDGGTGNEIELENREHHKSIDK 89
           AEPP+ RKLG+H           E P  E +   +S         E  +    HH ++++
Sbjct: 25  AEPPATRKLGRH-----------EWPGEEAEAPEVSHL-------EETVRRGHHHSTVER 84

Query: 90  SVAGGGVILGGLATTFLVAI 110
           SVAGGGVILGGLATTFLV +
Sbjct: 85  SVAGGGVILGGLATTFLVVV 86

The following BLAST results are available for this feature:

BLAST of CmaCh03G003940.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M330	7.9e-82	56.27	Probable proteasome inhibitor OS=Arabidopsis thaliana OX=3702 GN=At3g53970 PE=1 ...	[more]
Q92530	2.1e-05	28.02	Proteasome inhibitor PI31 subunit OS=Homo sapiens OX=9606 GN=PSMF1 PE=1 SV=2	[more]
Q5RDN3	3.9e-04	27.19	Proteasome inhibitor PI31 subunit OS=Pongo abelii OX=9601 GN=PSMF1 PE=2 SV=1	[more]

BLAST of CmaCh03G003940.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GF71	1.2e-173	95.92	probable proteasome inhibitor isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1ISG6	6.5e-172	100.00	probable proteasome inhibitor isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1GF52	9.5e-155	88.40	probable proteasome inhibitor isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1ITK3	1.3e-151	91.48	probable proteasome inhibitor isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1GE95	2.9e-151	85.89	probable proteasome inhibitor isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of CmaCh03G003940.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022950229.1	2.5e-173	95.92	probable proteasome inhibitor isoform X1 [Cucurbita moschata]	[more]
XP_022978238.1	1.3e-171	100.00	probable proteasome inhibitor isoform X1 [Cucurbita maxima]	[more]
XP_023545137.1	5.0e-166	96.72	probable proteasome inhibitor isoform X1 [Cucurbita pepo subsp. pepo]	[more]
KAG6603621.1	3.0e-155	94.97	putative proteasome inhibitor, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022950230.1	2.0e-154	88.40	probable proteasome inhibitor isoform X2 [Cucurbita moschata]	[more]

BLAST of CmaCh03G003940.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53970.1	5.6e-83	56.27	proteasome inhibitor-related	[more]
AT3G53970.2	1.6e-61	52.53	proteasome inhibitor-related	[more]
AT1G48530.1	1.1e-30	45.45	CONTAINS InterPro DOMAIN/s: Proteasome Inhibitor PI31 (InterPro:IPR021625); BEST...	[more]
AT3G09280.1	4.5e-08	42.50	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.1000.30		coord: 146..313 e-value: 3.3E-39 score: 135.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 28..77
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 429..450
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 300..338
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 300..327
IPR021625	PI31 proteasome regulator, N-terminal	PFAM	PF11566	PI31_Prot_N	coord: 160..299 e-value: 3.6E-14 score: 52.8
IPR045128	Proteasome inhibitor PI31-like	PANTHER	PTHR13266	PROTEASOME INHIBITOR	coord: 146..446

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh03G003940	CmaCh03G003940	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh03G003940.1:exon:69	CmaCh03G003940.1:exon:69	exon
CmaCh03G003940.1:exon:68	CmaCh03G003940.1:exon:68	exon
CmaCh03G003940.1:exon:67	CmaCh03G003940.1:exon:67	exon
CmaCh03G003940.1:exon:66	CmaCh03G003940.1:exon:66	exon
CmaCh03G003940.1:exon:65	CmaCh03G003940.1:exon:65	exon
CmaCh03G003940.1:exon:64	CmaCh03G003940.1:exon:64	exon
CmaCh03G003940.1:exon:63	CmaCh03G003940.1:exon:63	exon
CmaCh03G003940.1:exon:62	CmaCh03G003940.1:exon:62	exon
CmaCh03G003940.1:exon:61	CmaCh03G003940.1:exon:61	exon
CmaCh03G003940.1:exon:60	CmaCh03G003940.1:exon:60	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh03G003940.1:three_prime_utr	CmaCh03G003940.1:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh03G003940.1:cds	CmaCh03G003940.1:cds_10	CDS
CmaCh03G003940.1:cds	CmaCh03G003940.1:cds_9	CDS
CmaCh03G003940.1:cds	CmaCh03G003940.1:cds_8	CDS
CmaCh03G003940.1:cds	CmaCh03G003940.1:cds_7	CDS
CmaCh03G003940.1:cds	CmaCh03G003940.1:cds_6	CDS
CmaCh03G003940.1:cds	CmaCh03G003940.1:cds_5	CDS
CmaCh03G003940.1:cds	CmaCh03G003940.1:cds_4	CDS
CmaCh03G003940.1:cds	CmaCh03G003940.1:cds_3	CDS
CmaCh03G003940.1:cds	CmaCh03G003940.1:cds_2	CDS
CmaCh03G003940.1:cds	CmaCh03G003940.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh03G003940.1	CmaCh03G003940.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0070628	proteasome binding