CmaCh03G003800.1 (mRNA) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh03G003800.1 vs. ExPASy Swiss-Prot
Match: Q9SR40 (Laccase-7 OS=Arabidopsis thaliana OX=3702 GN=LAC7 PE=2 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 7.9e-212
Identity = 357/566 (63.07%), Postives = 432/566 (76.33%), Query Frame = 0

Query: 13  LLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHVHDGDILVVHVS 72
           L+LLA SS+ SA+IVEH+F VQ++TV RLC+ Q IT VNG  PGPTI V +GD LV+HV 
Sbjct: 12  LILLAISSITSASIVEHTFNVQNLTVSRLCKRQVITVVNGSLPGPTIRVKEGDSLVIHVL 71

Query: 73  NKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAHSSW 132
           N SP+++TIHWHG+F  L+ WADGP  +TQCPI+PG  Y YRF I  QEGTLWWHAH+S+
Sbjct: 72  NHSPHNITIHWHGIFHKLTVWADGPSMITQCPIQPGQRYAYRFNITGQEGTLWWHAHASF 131

Query: 133 LRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNISDAY 192
           LRATV+GAL+IRPKSG   P+PKP+K++PIL GEWWN +VV +EE  +ATG  PN SDAY
Sbjct: 132 LRATVYGALVIRPKSGHSYPFPKPHKEVPILFGEWWNTDVVALEEAAIATGVPPNNSDAY 191

Query: 193 TINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYT 252
           TING PGNLYPCS+++ ++L + +GK YLL++INAA+N Q FFK+ANH LTVVA DAVYT
Sbjct: 192 TINGRPGNLYPCSKDRMFSLNVVKGKRYLLRIINAAMNIQLFFKIANHRLTVVAADAVYT 251

Query: 253 DPYVTDVIVLAPGQTTDVLVKADQP-PRSYYMAARPYADARPPIDFSDTITRAIVVYNGA 312
            PYVTDVIV+APGQT D L+ ADQ    SYYMAA PYA A P + F +T TR ++ Y GA
Sbjct: 252 APYVTDVIVIAPGQTIDALLFADQSVDTSYYMAAHPYASA-PAVPFPNTTTRGVIHYGGA 311

Query: 313 SP---SAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFVTFGLNLAP 372
           S    S   LMP LP F DT TA++FY+N+TALV   HWVPVPR+VD  M VT GL L  
Sbjct: 312 SKTGRSKPVLMPKLPSFFDTLTAYRFYSNLTALVNGPHWVPVPRYVDEEMLVTIGLGLEA 371

Query: 373 CGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDFPDQPPVEF 432
           C   + T C      + SASM+N SFV+P    LS+LEA FH VKG+++ DFPDQPPV+F
Sbjct: 372 CA--DNTTC-----PKFSASMSNHSFVLPKK--LSILEAVFHDVKGIFTADFPDQPPVKF 431

Query: 433 DYTNSSIRLDN-SLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLA 492
           DYTN ++   N  L+F  K+TS K LKFN+TVE+VLQN A ++ E+HPMHLHGFNFHVLA
Sbjct: 432 DYTNPNVTQTNPGLLFTQKSTSAKILKFNTTVEVVLQNHALIAAESHPMHLHGFNFHVLA 491

Query: 493 QGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGL 552
           QGFGNYDP  D  K N ++PQ RNT+AVPV GWAVIRF ANNPG W+ HCH+DVH+P+GL
Sbjct: 492 QGFGNYDPSRDRSKLNLVDPQSRNTLAVPVGGWAVIRFTANNPGAWIFHCHIDVHLPFGL 551

Query: 553 AMGFEVENGPTPSTWLPPPPHDLPKC 574
            M F V+NGPT ST LPPPP DLPKC
Sbjct: 552 GMIFVVKNGPTKSTTLPPPPPDLPKC 567

BLAST of CmaCh03G003800.1 vs. ExPASy Swiss-Prot
Match: Q9LFD1 (Laccase-9 OS=Arabidopsis thaliana OX=3702 GN=LAC9 PE=2 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 6.7e-195
Identity = 323/565 (57.17%), Postives = 410/565 (72.57%), Query Frame = 0

Query: 13  LLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHVHDGDILVVHVS 72
           L+LL  SS+ASAAIVEH   V+D+ V  LC++Q I  VNG  PGPTI+V +GD LVVHV 
Sbjct: 14  LVLLLFSSIASAAIVEHVLHVKDVVVTPLCKEQMIPIVNGSLPGPTINVREGDTLVVHVI 73

Query: 73  NKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAHSSW 132
           NKS Y++TIHWHGVFQL S W DG   +TQCPI+P  N+TY+F I  QEGTL WHAH   
Sbjct: 74  NKSTYNVTIHWHGVFQLKSVWMDGANMITQCPIQPSNNFTYQFDITGQEGTLLWHAHVVN 133

Query: 133 LRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNISDAY 192
           LRAT+HGAL+IRP+SGRP P+PKPYK++P++  +WW+ +V  +E           +SDAY
Sbjct: 134 LRATIHGALIIRPRSGRPYPFPKPYKEVPLIFQQWWDTDVRLLELRPAP------VSDAY 193

Query: 193 TINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYT 252
            INGL G+ YPCS+N+ + LK+ +GKTYLL++INAALN   FFK+ANHN+TVVAVDAVYT
Sbjct: 194 LINGLAGDSYPCSKNRMFNLKVVQGKTYLLRIINAALNTHLFFKIANHNVTVVAVDAVYT 253

Query: 253 DPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADA-RPPIDFSDTITRAIVVYNGA 312
            PY+TDV++L PGQT D ++ ADQP  +YYMA  PY  A   P       TR ++VY GA
Sbjct: 254 TPYLTDVMILTPGQTIDAILTADQPIGTYYMAIIPYFSAIGVPASPDTKPTRGLIVYEGA 313

Query: 313 SPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFVTFGLNLAPCGL 372
           + S++P  P +PP ND PTAH+F +NIT+LVG  HW PVPRHVD  MF+T GL L PC  
Sbjct: 314 TSSSSPTKPWMPPANDIPTAHRFSSNITSLVGGPHWTPVPRHVDEKMFITMGLGLDPC-- 373

Query: 373 PNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDFPDQPPVEFDYT 432
           P+   C GP  QRL+ S+NN +F+IP    +SM EA+F+ + GVY+ DFPDQPP++FD+T
Sbjct: 374 PSNAKCVGPLDQRLAGSLNNRTFMIPE--RISMQEAYFYNITGVYTDDFPDQPPLKFDFT 433

Query: 433 NSSIRLDNS---LIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLAQ 492
                  NS   ++F  + TSVK ++FNSTVE+VLQNT  ++ E+HPMHLHGFNF+VL  
Sbjct: 434 KFEQHPTNSDMEMMFPERKTSVKTIRFNSTVEIVLQNTGILTPESHPMHLHGFNFYVLGY 493

Query: 493 GFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGLA 552
           GFGNYDPI D +K N  NPQ+ NT+ VP  GW V+RF ANNPG+WL HCH+D H+P G+ 
Sbjct: 494 GFGNYDPIRDARKLNLFNPQMHNTVGVPPGGWVVLRFIANNPGIWLFHCHMDAHLPLGIM 553

Query: 553 MGFEVENGPTPSTWLPPPPHDLPKC 574
           M F V+NGPT  T LP PP +LP+C
Sbjct: 554 MAFIVQNGPTRETSLPSPPSNLPQC 568

BLAST of CmaCh03G003800.1 vs. ExPASy Swiss-Prot
Match: Q9LFD2 (Laccase-8 OS=Arabidopsis thaliana OX=3702 GN=LAC8 PE=2 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 8.8e-195
Identity = 325/565 (57.52%), Postives = 413/565 (73.10%), Query Frame = 0

Query: 13  LLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHVHDGDILVVHVS 72
           L+LL  SS+ASAA+VEH   +QD+ V+ LC++Q I A NG  PGPTI+V +GD LVV+V 
Sbjct: 14  LVLLLFSSIASAAVVEHVLHIQDVVVKPLCKEQIIPAANGSLPGPTINVREGDTLVVNVI 73

Query: 73  NKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAHSSW 132
           N S Y++TIHWHGVFQL S W DG   +TQCPI+PG N+TY+F I  QEGTL WHAH   
Sbjct: 74  NNSTYNVTIHWHGVFQLKSVWMDGANMITQCPIQPGYNFTYQFDITGQEGTLLWHAHVVN 133

Query: 133 LRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNISDAY 192
           LRAT+HGAL+IRP+SGRP P+PKPYK++PI+  +WW+ +V       L     P +SDAY
Sbjct: 134 LRATLHGALVIRPRSGRPYPFPKPYKEVPIVFQQWWDTDV-----RLLQLRPAP-VSDAY 193

Query: 193 TINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYT 252
            INGL G+ YPCS+N+ + LK+ +GKTYLL+++NAALN   FFK+ANHN+TVVAVDAVY+
Sbjct: 194 LINGLAGDSYPCSENRMFNLKVVQGKTYLLRIVNAALNTHLFFKIANHNVTVVAVDAVYS 253

Query: 253 DPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADA--RPPIDFSDTITRAIVVYNG 312
            PY+TDV++L PGQT D L+ ADQ    YYMA  PY  A   P  D     TR ++VY G
Sbjct: 254 TPYLTDVMILTPGQTVDALLTADQAIGKYYMATLPYISAIGIPTPDIKP--TRGLIVYQG 313

Query: 313 ASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFVTFGLNLAPCG 372
           A+ S++P  P +P  ND  TAH+F +NIT+LVG  HW PVPRHVD  MF+T GL L PC 
Sbjct: 314 ATSSSSPAEPLMPVPNDMSTAHRFTSNITSLVGGPHWTPVPRHVDEKMFITMGLGLDPC- 373

Query: 373 LPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDFPDQPPVEFDY 432
            P GT C GP GQR + S+NN +F+IP    +SM EA+F+ + G+Y+ DFP+QPP++FDY
Sbjct: 374 -PAGTKCIGPLGQRYAGSLNNRTFMIPE--RISMQEAYFYNISGIYTDDFPNQPPLKFDY 433

Query: 433 TNSSIRLDN--SLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLAQ 492
           T    R +N   ++F  + TSVKK++FNSTVE+VLQNTA +S E+HPMHLHGFNF+VL  
Sbjct: 434 TKFEQRTNNDMKMMFPERKTSVKKIRFNSTVEIVLQNTAIISPESHPMHLHGFNFYVLGY 493

Query: 493 GFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGLA 552
           GFGNYDPI D +K N  NPQ+ NT+ VP  GW V+RF ANNPGVWL HCH+D H+P+G+ 
Sbjct: 494 GFGNYDPIRDARKLNLFNPQMHNTVGVPPGGWVVLRFIANNPGVWLFHCHMDAHLPYGIM 553

Query: 553 MGFEVENGPTPSTWLPPPPHDLPKC 574
             F V+NGPTP T LP PP +LP+C
Sbjct: 554 SAFIVQNGPTPETSLPSPPSNLPQC 566

BLAST of CmaCh03G003800.1 vs. ExPASy Swiss-Prot
Match: Q9FLB5 (Laccase-12 OS=Arabidopsis thaliana OX=3702 GN=LAC12 PE=2 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 1.8e-176
Identity = 303/568 (53.35%), Postives = 397/568 (69.89%), Query Frame = 0

Query: 9   LLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHVHDGDILV 68
           +LL    L S+S+  A +  H F +Q+  V+RLC+ +    VNG +PGPT+ V++GD L 
Sbjct: 9   ILLFFCSLFSASLIIAKVQHHDFVIQETPVKRLCKTRNAITVNGMFPGPTLEVNNGDTLE 68

Query: 69  VHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHA 128
           V V N++ Y++TIHWHGV Q+ +GWADGPE VTQCPIRPG +YTYRF I+ QEGTLWWHA
Sbjct: 69  VKVHNRARYNITIHWHGVRQIRTGWADGPEFVTQCPIRPGKSYTYRFTIQGQEGTLWWHA 128

Query: 129 HSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNI 188
           HSSWLRATV+GAL+I P  G   P+PKP ++  ++LGEWWNAN V V  +   TG+ PNI
Sbjct: 129 HSSWLRATVYGALIIHPTPGSSFPFPKPDRQTALMLGEWWNANPVDVINQATRTGAAPNI 188

Query: 189 SDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVD 248
           SDAYTING PG+LY CS  +T  + +  G+T LL+VINAALN   FF +ANH LTVV  D
Sbjct: 189 SDAYTINGQPGDLYNCSTKETVVVPINSGETSLLRVINAALNQPLFFTVANHKLTVVGAD 248

Query: 249 AVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDTITRAIVVY 308
           A Y  P+ T V++L PGQTTDVL+ ADQPP+ YY+AAR Y  A+    F +T T AI+ Y
Sbjct: 249 ASYLKPFTTKVLMLGPGQTTDVLLTADQPPKRYYIAARAYQSAQ-NAPFDNTTTTAILQY 308

Query: 309 NGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFVTFGLNLAP 368
              + ++ P+MP LP FNDT T   F     +L      V VP+ +D ++F T GL L  
Sbjct: 309 KKTTTTSKPIMPVLPAFNDTNTVTSFSRKFKSLRN----VVVPKTIDDNLFFTIGLGLDN 368

Query: 369 C--GLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDFPDQPPV 428
           C    P  + C G NG R +ASMNNVSFV+P++   S+L+A  + + GV++TDFP +PPV
Sbjct: 369 CPKKFPK-SRCQGLNGTRFTASMNNVSFVLPSN--FSLLQAHSNGIPGVFTTDFPSKPPV 428

Query: 429 EFDYTNSSIRLDNSLIFAP-KATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHV 488
           +FDYT ++I   +  +F P K T + KLK+ S V++VLQ+T  V+ ENHP+HLHG++F++
Sbjct: 429 KFDYTGNNI---SRALFQPVKGTKLYKLKYGSRVQVVLQDTNIVTSENHPIHLHGYDFYI 488

Query: 489 LAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPW 548
           + +GFGN++P  D  KFN ++P +RNT+AVPV GWAVIRF A+NPGVWLMHCHLDVH+ W
Sbjct: 489 VGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGVWLMHCHLDVHIKW 548

Query: 549 GLAMGFEVENGPTPSTWLPPPPHDLPKC 574
           GLAM F V+NG      L  PPHDLP C
Sbjct: 549 GLAMAFLVDNGVGELETLEAPPHDLPIC 565

BLAST of CmaCh03G003800.1 vs. ExPASy Swiss-Prot
Match: Q2QUN2 (Laccase-24 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC24 PE=2 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 1.6e-175
Identity = 311/580 (53.62%), Postives = 403/580 (69.48%), Query Frame = 0

Query: 8   LLLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQE-ITAVNGEYPGPTIHVHDGDI 67
           LLLL   L   +S+A AA+VE++F V ++++ +LC+ +  ITAVNG+ PGPTI   +GD 
Sbjct: 7   LLLLPFALALVASVAQAAVVEYTFNVGNLSISQLCQQEMIITAVNGQLPGPTIVATEGDT 66

Query: 68  LVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWW 127
           +VVH+ N+SPY++TIHWHG+FQ  + WADGP  VTQCP+RPGGNYTYRF +  QEGTLWW
Sbjct: 67  VVVHMVNESPYNMTIHWHGIFQRGTPWADGPAMVTQCPVRPGGNYTYRFNVTGQEGTLWW 126

Query: 128 HAHSSWLRATVHGALLIRPKSG-RPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSG 187
           H+H S+LRATV+GAL+I+P+ G +  P+P P +++ ++LGEWW  NV  +++  L TG+ 
Sbjct: 127 HSHFSFLRATVYGALIIKPRGGAKAYPFPVPDEEVVVILGEWWKTNVYDLQQRSLVTGNP 186

Query: 188 PNISDAYTINGLPGNLYPCS-QNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTV 247
              +DAYTING PG+ Y CS  NQT+  ++ + KTY+L++INAALN   FFK+ANH+  V
Sbjct: 187 APHADAYTINGKPGDFYNCSAPNQTHKFELKQNKTYMLRIINAALNTPLFFKVANHSFNV 246

Query: 248 VAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRS-----YYMAARPYAD----ARPPI 307
           VA DA YT PY TDV+V++PGQT D L+  D    +     YYMA  PY      A P  
Sbjct: 247 VAADACYTKPYKTDVVVISPGQTVDALLVPDAGVAAAVGGRYYMAVIPYNSAVNAADPSF 306

Query: 308 DFSDTITRAIVVYNGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVD 367
            +S T + AIV Y G   ++ P++P +P +NDT TAH+F +N+TALV  R    VP  VD
Sbjct: 307 LYSLTNSTAIVEYGGGPATSPPMVPDMPEYNDTATAHRFLSNMTALVPNR----VPLAVD 366

Query: 368 HHMFVTFGLNLAPCGLPNGTAC-GGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFH-KVK 427
            HMFVT  +    CG P  T C     G   ++SMNN SF++PN    SMLEA +   + 
Sbjct: 367 THMFVTVSMGDTFCG-PEQTMCMPDDKGTIFASSMNNASFILPNTT--SMLEAMYKGSID 426

Query: 428 GVYSTDFPDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLEN 487
           GVY+ DFPD PP+ FDYT  +   + +L    K+T VK LK+NSTV+MVLQNT  VS E+
Sbjct: 427 GVYTRDFPDTPPIVFDYTADASDDNATLKHTFKSTKVKTLKYNSTVQMVLQNTRLVSKES 486

Query: 488 HPMHLHGFNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVW 547
           HPMHLHGFNF VLAQGFGNY+   DP KFN ++PQ RNT+AVP  GWAVIRF A+NPGVW
Sbjct: 487 HPMHLHGFNFFVLAQGFGNYNETTDPAKFNLVDPQERNTVAVPTGGWAVIRFVADNPGVW 546

Query: 548 LMHCHLDVHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 574
            MHCH D H+ +GL M FEV+NGPT  T LPPPP DLP+C
Sbjct: 547 FMHCHFDAHLEFGLGMVFEVQNGPTQETSLPPPPSDLPQC 579

BLAST of CmaCh03G003800.1 vs. ExPASy TrEMBL
Match: A0A6J1IKA9 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111478200 PE=3 SV=1)

HSP 1 Score: 1200.7 bits (3105), Expect = 0.0e+00
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MASSCLPLLLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH 60
           MASSCLPLLLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH
Sbjct: 1   MASSCLPLLLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH 60

Query: 61  VHDGDILVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQ 120
           VHDGDILVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQ
Sbjct: 61  VHDGDILVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQ 120

Query: 121 EGTLWWHAHSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 180
           EGTLWWHAHSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL
Sbjct: 121 EGTLWWHAHSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 180

Query: 181 ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH 240
           ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH
Sbjct: 181 ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH 240

Query: 241 NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT 300
           NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT
Sbjct: 241 NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT 300

Query: 301 ITRAIVVYNGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFV 360
           ITRAIVVYNGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFV
Sbjct: 301 ITRAIVVYNGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFV 360

Query: 361 TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDF 420
           TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDF
Sbjct: 361 TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDF 420

Query: 421 PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG 480
           PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG
Sbjct: 421 PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG 480

Query: 481 FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD 540
           FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD
Sbjct: 481 FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD 540

Query: 541 VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 574
           VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC
Sbjct: 541 VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 573

BLAST of CmaCh03G003800.1 vs. ExPASy TrEMBL
Match: A0A6J1GGH4 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453717 PE=3 SV=1)

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 559/573 (97.56%), Postives = 566/573 (98.78%), Query Frame = 0

Query: 1   MASSCLPLLLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH 60
           MASS LP  LLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVN EYPGPTIH
Sbjct: 1   MASSSLP--LLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNSEYPGPTIH 60

Query: 61  VHDGDILVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQ 120
            HDGD+LVVHVSNKSPYDLTIHWHG+FQLLS WADGPENVTQCPIRPGGNYTY+FKIKEQ
Sbjct: 61  AHDGDVLVVHVSNKSPYDLTIHWHGIFQLLSAWADGPENVTQCPIRPGGNYTYKFKIKEQ 120

Query: 121 EGTLWWHAHSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 180
           EGTLWWHAHSSWLRATVHGALLIRPK+GRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL
Sbjct: 121 EGTLWWHAHSSWLRATVHGALLIRPKNGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 180

Query: 181 ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH 240
           ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH
Sbjct: 181 ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH 240

Query: 241 NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT 300
           NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT
Sbjct: 241 NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT 300

Query: 301 ITRAIVVYNGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFV 360
           ITRAIVVY+GASPSAAPLMPTLP FNDTPTAHKFYTNIT+LVGARHWVPVPRHVDHHMFV
Sbjct: 301 ITRAIVVYDGASPSAAPLMPTLPSFNDTPTAHKFYTNITSLVGARHWVPVPRHVDHHMFV 360

Query: 361 TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDF 420
           TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEA+FHKVKGVYSTDF
Sbjct: 361 TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAYFHKVKGVYSTDF 420

Query: 421 PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG 480
           PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG
Sbjct: 421 PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG 480

Query: 481 FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD 540
           FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD
Sbjct: 481 FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD 540

Query: 541 VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 574
           VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC
Sbjct: 541 VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 571

BLAST of CmaCh03G003800.1 vs. ExPASy TrEMBL
Match: A0A6J1GEQ7 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453486 PE=3 SV=1)

HSP 1 Score: 1048.1 bits (2709), Expect = 1.3e-302
Identity = 503/573 (87.78%), Postives = 524/573 (91.45%), Query Frame = 0

Query: 1   MASSCLPLLLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH 60
           MASS   L LLLLLLLASSSMASAAIVEHSFTV+DMTVRRLCRDQ ITAVNGEYPGPTIH
Sbjct: 1   MASS---LALLLLLLLASSSMASAAIVEHSFTVEDMTVRRLCRDQVITAVNGEYPGPTIH 60

Query: 61  VHDGDILVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQ 120
           V D D+L+VHVSN SPYDLTIHWHGVFQLLS WADGPENVTQCPIRPGGNYTYRF IK Q
Sbjct: 61  VQDDDVLIVHVSNNSPYDLTIHWHGVFQLLSAWADGPENVTQCPIRPGGNYTYRFNIKGQ 120

Query: 121 EGTLWWHAHSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 180
           EGTLWWHAHSSWLRATVHGALLIRPKSG PLPY KPYKK+PILLGEWWNANVVHVEEEGL
Sbjct: 121 EGTLWWHAHSSWLRATVHGALLIRPKSGLPLPYQKPYKKVPILLGEWWNANVVHVEEEGL 180

Query: 181 ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH 240
           A G GPN SDAYTINGLPGNLYPC QNQTY LKMARGKT LLQV+NAALNNQ FFKLANH
Sbjct: 181 AAGRGPNGSDAYTINGLPGNLYPCPQNQTYQLKMARGKTSLLQVVNAALNNQLFFKLANH 240

Query: 241 NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT 300
           NLTVVAVDA YT PYVTDVIVLAPGQTTDVLV ADQP  SYYMAARPYAD RP I F +T
Sbjct: 241 NLTVVAVDATYTVPYVTDVIVLAPGQTTDVLVNADQPLGSYYMAARPYADTRPQIAFPNT 300

Query: 301 ITRAIVVYNGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFV 360
           ITRAIV+Y+GA  ++ P+MP LP FNDTPTAHKFYTN+TALVGARHW P PRHVDHHMFV
Sbjct: 301 ITRAIVIYDGAPTNSTPIMPLLPAFNDTPTAHKFYTNLTALVGARHWDPAPRHVDHHMFV 360

Query: 361 TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDF 420
           TF LNLAPCG PNG  CGGPNGQRLSASM NVSFVIPNDAGLSMLEAFFHKV+GVY+ DF
Sbjct: 361 TFRLNLAPCGTPNG--CGGPNGQRLSASMXNVSFVIPNDAGLSMLEAFFHKVEGVYTGDF 420

Query: 421 PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG 480
           PD PPVEFDYTNSSIRLDNSLIFA KATSVKKLKFNSTVE+VLQNTAF++ ENHP+HLHG
Sbjct: 421 PDLPPVEFDYTNSSIRLDNSLIFAAKATSVKKLKFNSTVEIVLQNTAFIAKENHPLHLHG 480

Query: 481 FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD 540
           FNFHVLAQGFGNYDPIHDP+KFNFLNPQIRNTIAVP+ GW VIRFQANNPGVWLMHCHLD
Sbjct: 481 FNFHVLAQGFGNYDPIHDPEKFNFLNPQIRNTIAVPIGGWPVIRFQANNPGVWLMHCHLD 540

Query: 541 VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 574
           VH+PWGLAMGFEV NGPTPST LPPPPHDLPKC
Sbjct: 541 VHLPWGLAMGFEVANGPTPSTRLPPPPHDLPKC 568

BLAST of CmaCh03G003800.1 vs. ExPASy TrEMBL
Match: A0A1S3BPD5 (Laccase OS=Cucumis melo OX=3656 GN=LOC103491871 PE=3 SV=1)

HSP 1 Score: 1028.5 bits (2658), Expect = 1.1e-296
Identity = 489/573 (85.34%), Postives = 519/573 (90.58%), Query Frame = 0

Query: 3   SSCLPLLLLLLLLLASSS--MASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH 62
           +SCL LLLLL L  +SSS  M SAAIVEHSFTV+DM+VRRLCRDQ ITAVNGEYPGPTIH
Sbjct: 2   ASCLVLLLLLFLASSSSSYTMVSAAIVEHSFTVEDMSVRRLCRDQVITAVNGEYPGPTIH 61

Query: 63  VHDGDILVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQ 122
           V D D+LVVHV+N SP+DLTIHWHGVFQLLSGWADGPEN+TQCPIRPG  YTYRFKIK Q
Sbjct: 62  VQDEDVLVVHVTNNSPHDLTIHWHGVFQLLSGWADGPENITQCPIRPGKKYTYRFKIKGQ 121

Query: 123 EGTLWWHAHSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 182
           EGTLWWHAHSSWLRATVHGALLIRPK   PLPYP PYKKIPILLGEWWNANVV VE+EGL
Sbjct: 122 EGTLWWHAHSSWLRATVHGALLIRPKFDLPLPYPTPYKKIPILLGEWWNANVVQVEQEGL 181

Query: 183 ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH 242
           ATG GPN SDAYTING PGNLYPCSQNQTY LKM RGKTYLLQVIN ALNNQ FFKLANH
Sbjct: 182 ATGRGPNGSDAYTINGHPGNLYPCSQNQTYELKMVRGKTYLLQVINVALNNQLFFKLANH 241

Query: 243 NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT 302
             TVVAVDA YTDPY+TDVIVLAPGQTTDVLVKA+QP  SYYMAARPYAD +P I F ++
Sbjct: 242 KFTVVAVDATYTDPYITDVIVLAPGQTTDVLVKANQPIGSYYMAARPYADTQPQIGFPNS 301

Query: 303 ITRAIVVYNGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFV 362
           ITRA+V+Y+GASPS  P+MPTLP FNDTPTAHKFYTNITALVGARHWVPVPRHVD+HMFV
Sbjct: 302 ITRAVVIYDGASPSTTPIMPTLPGFNDTPTAHKFYTNITALVGARHWVPVPRHVDNHMFV 361

Query: 363 TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDF 422
           TFGLNLA CG  NG+ CGGPNGQRLSASMNNVSFVIPNDAGLSMLEA+FHKV+GVYS DF
Sbjct: 362 TFGLNLASCGAVNGSTCGGPNGQRLSASMNNVSFVIPNDAGLSMLEAYFHKVEGVYSRDF 421

Query: 423 PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG 482
           PD+P V+FDYTNSS+ LDNSLIFAPK T VKKLKFNSTVE+VLQNTAF++ ENHPMHLHG
Sbjct: 422 PDEPTVKFDYTNSSLGLDNSLIFAPKGTKVKKLKFNSTVEIVLQNTAFIAKENHPMHLHG 481

Query: 483 FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD 542
           FNFHVLAQGFGNYDPI DP  FNF+NPQIRNTIAVPV GWAVIRFQANNPGVWLMHCHLD
Sbjct: 482 FNFHVLAQGFGNYDPIRDPYMFNFVNPQIRNTIAVPVGGWAVIRFQANNPGVWLMHCHLD 541

Query: 543 VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 574
           VH+PWGLAMGFEVENGPTPST LPPPP DLPKC
Sbjct: 542 VHLPWGLAMGFEVENGPTPSTRLPPPPSDLPKC 574

BLAST of CmaCh03G003800.1 vs. ExPASy TrEMBL
Match: A0A6J1DEW8 (Laccase OS=Momordica charantia OX=3673 GN=LOC111019442 PE=3 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 4.0e-291
Identity = 476/564 (84.40%), Postives = 510/564 (90.43%), Query Frame = 0

Query: 10  LLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHVHDGDILVV 69
           L  L LLASSSMASAAI++HSFTVQDMTVRRLCRDQ ITAVNG  PGPTIH  DGD+LVV
Sbjct: 37  LFALFLLASSSMASAAILQHSFTVQDMTVRRLCRDQVITAVNGNXPGPTIHAQDGDVLVV 96

Query: 70  HVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAH 129
           HV NKSPYDLTIHWHGVFQLLS WADGPENVTQCPIR G NYTYRFKI  QEGTLWWHAH
Sbjct: 97  HVFNKSPYDLTIHWHGVFQLLSAWADGPENVTQCPIRSGENYTYRFKITGQEGTLWWHAH 156

Query: 130 SSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNIS 189
           SSWLRATVHGALLI PKSGRPLP+PKPY++IPILLGEWWNANVV VEE GLATG+GPN S
Sbjct: 157 SSWLRATVHGALLIAPKSGRPLPFPKPYRQIPILLGEWWNANVVEVEEGGLATGAGPNNS 216

Query: 190 DAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDA 249
           DAYTIN LPGNLYPCSQNQTY LKM   KTYLLQVINAALNNQ FFKLANH +TVVAVDA
Sbjct: 217 DAYTINALPGNLYPCSQNQTYQLKMVPKKTYLLQVINAALNNQLFFKLANHKMTVVAVDA 276

Query: 250 VYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDTITRAIVVYN 309
           +YTDPYVTDVIVLAPGQTTDVLVKADQP  SYYMAA PY+D RPPI F ++ITRA+V+Y+
Sbjct: 277 MYTDPYVTDVIVLAPGQTTDVLVKADQPIGSYYMAASPYSDLRPPIPFPNSITRAVVIYD 336

Query: 310 GASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFVTFGLNLAPC 369
           GASPSA P MP LP FNDTPTAH+F+TNITAL GARHWVPVP HVD HMFVTFG+NLAPC
Sbjct: 337 GASPSATPRMPVLPAFNDTPTAHRFFTNITALAGARHWVPVPXHVDEHMFVTFGVNLAPC 396

Query: 370 GLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDFPDQPPVEFD 429
             PN   CGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFH+V+GVY+ DFPD+PPVEFD
Sbjct: 397 EEPN-RPCGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHRVEGVYTADFPDRPPVEFD 456

Query: 430 YTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLAQG 489
           YTN +I +++ LIFAPKAT VKKLKFNSTVEMVLQNTAF+++ NHP+HLH FNFHVLAQG
Sbjct: 457 YTNPNISVESPLIFAPKATKVKKLKFNSTVEMVLQNTAFIAMGNHPLHLHRFNFHVLAQG 516

Query: 490 FGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGLAM 549
           FGNYDPI+DPKKFNF NPQIRNT+AVPV GWAVIRFQANNPGVWLMHCHLDVH+PWGLAM
Sbjct: 517 FGNYDPINDPKKFNFYNPQIRNTVAVPVGGWAVIRFQANNPGVWLMHCHLDVHLPWGLAM 576

Query: 550 GFEVENGPTPSTWLPPPPHDLPKC 574
           GFEVENGP PS+ LPPPPHDLPKC
Sbjct: 577 GFEVENGPAPSSRLPPPPHDLPKC 599

BLAST of CmaCh03G003800.1 vs. NCBI nr
Match: XP_022978127.1 (laccase-7-like [Cucurbita maxima])

HSP 1 Score: 1200.7 bits (3105), Expect = 0.0e+00
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MASSCLPLLLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH 60
           MASSCLPLLLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH
Sbjct: 1   MASSCLPLLLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH 60

Query: 61  VHDGDILVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQ 120
           VHDGDILVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQ
Sbjct: 61  VHDGDILVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQ 120

Query: 121 EGTLWWHAHSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 180
           EGTLWWHAHSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL
Sbjct: 121 EGTLWWHAHSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 180

Query: 181 ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH 240
           ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH
Sbjct: 181 ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH 240

Query: 241 NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT 300
           NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT
Sbjct: 241 NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT 300

Query: 301 ITRAIVVYNGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFV 360
           ITRAIVVYNGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFV
Sbjct: 301 ITRAIVVYNGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFV 360

Query: 361 TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDF 420
           TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDF
Sbjct: 361 TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDF 420

Query: 421 PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG 480
           PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG
Sbjct: 421 PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG 480

Query: 481 FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD 540
           FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD
Sbjct: 481 FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD 540

Query: 541 VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 574
           VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC
Sbjct: 541 VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 573

BLAST of CmaCh03G003800.1 vs. NCBI nr
Match: XP_022950700.1 (laccase-7-like [Cucurbita moschata])

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 559/573 (97.56%), Postives = 566/573 (98.78%), Query Frame = 0

Query: 1   MASSCLPLLLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH 60
           MASS LP  LLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVN EYPGPTIH
Sbjct: 1   MASSSLP--LLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNSEYPGPTIH 60

Query: 61  VHDGDILVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQ 120
            HDGD+LVVHVSNKSPYDLTIHWHG+FQLLS WADGPENVTQCPIRPGGNYTY+FKIKEQ
Sbjct: 61  AHDGDVLVVHVSNKSPYDLTIHWHGIFQLLSAWADGPENVTQCPIRPGGNYTYKFKIKEQ 120

Query: 121 EGTLWWHAHSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 180
           EGTLWWHAHSSWLRATVHGALLIRPK+GRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL
Sbjct: 121 EGTLWWHAHSSWLRATVHGALLIRPKNGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 180

Query: 181 ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH 240
           ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH
Sbjct: 181 ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH 240

Query: 241 NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT 300
           NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT
Sbjct: 241 NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT 300

Query: 301 ITRAIVVYNGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFV 360
           ITRAIVVY+GASPSAAPLMPTLP FNDTPTAHKFYTNIT+LVGARHWVPVPRHVDHHMFV
Sbjct: 301 ITRAIVVYDGASPSAAPLMPTLPSFNDTPTAHKFYTNITSLVGARHWVPVPRHVDHHMFV 360

Query: 361 TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDF 420
           TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEA+FHKVKGVYSTDF
Sbjct: 361 TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAYFHKVKGVYSTDF 420

Query: 421 PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG 480
           PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG
Sbjct: 421 PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG 480

Query: 481 FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD 540
           FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD
Sbjct: 481 FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD 540

Query: 541 VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 574
           VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC
Sbjct: 541 VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 571

BLAST of CmaCh03G003800.1 vs. NCBI nr
Match: XP_023543234.1 (laccase-7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 543/553 (98.19%), Postives = 548/553 (99.10%), Query Frame = 0

Query: 21  MASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHVHDGDILVVHVSNKSPYDLT 80
           MASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH HDGD+LVVHVSNKSPYDLT
Sbjct: 1   MASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHAHDGDVLVVHVSNKSPYDLT 60

Query: 81  IHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAHSSWLRATVHGA 140
           IHWHG+FQ LS WADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAHSSWLRATVHGA
Sbjct: 61  IHWHGIFQQLSAWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAHSSWLRATVHGA 120

Query: 141 LLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNISDAYTINGLPGN 200
           LLIRPK+GRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL TGSGPNISDAYTINGLPGN
Sbjct: 121 LLIRPKNGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLTTGSGPNISDAYTINGLPGN 180

Query: 201 LYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYTDPYVTDVI 260
           LYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYTDPYVTDVI
Sbjct: 181 LYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYTDPYVTDVI 240

Query: 261 VLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDTITRAIVVYNGASPSAAPLMP 320
           VLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDTITRAIVVY+GASPSAAPLMP
Sbjct: 241 VLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDTITRAIVVYDGASPSAAPLMP 300

Query: 321 TLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFVTFGLNLAPCGLPNGTACGGP 380
           TLP FNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFVTFGLNLAPCGLPNGTACGGP
Sbjct: 301 TLPSFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFVTFGLNLAPCGLPNGTACGGP 360

Query: 381 NGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDFPDQPPVEFDYTNSSIRLDNS 440
           NGQRLSASMNNVSFVIPNDAGLSMLEA+FHKVKGVYSTDFPDQPPVEFDYTNSSIRLDNS
Sbjct: 361 NGQRLSASMNNVSFVIPNDAGLSMLEAYFHKVKGVYSTDFPDQPPVEFDYTNSSIRLDNS 420

Query: 441 LIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLAQGFGNYDPIHDPK 500
           LIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLAQGFGNYDPIHDPK
Sbjct: 421 LIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLAQGFGNYDPIHDPK 480

Query: 501 KFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGLAMGFEVENGPTPS 560
           KFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGLAMGFEVENGPTPS
Sbjct: 481 KFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGLAMGFEVENGPTPS 540

Query: 561 TWLPPPPHDLPKC 574
           TWLPPPPHDLPKC
Sbjct: 541 TWLPPPPHDLPKC 553

BLAST of CmaCh03G003800.1 vs. NCBI nr
Match: XP_038883561.1 (laccase-7-like [Benincasa hispida])

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 519/573 (90.58%), Postives = 540/573 (94.24%), Query Frame = 0

Query: 1   MASSCLPLLLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH 60
           MASSC  L+ LLLLL +SSSM SAAIVEHSFTVQDMTVRRLCR+QEITAVNGEYPGPTIH
Sbjct: 1   MASSC--LMALLLLLASSSSMVSAAIVEHSFTVQDMTVRRLCREQEITAVNGEYPGPTIH 60

Query: 61  VHDGDILVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQ 120
           V DGD+LVVHV+N SPYDLTIHWHGVFQLLS WADGPEN+TQCPIRPGGNYTY+F+IKEQ
Sbjct: 61  VQDGDVLVVHVTNNSPYDLTIHWHGVFQLLSAWADGPENITQCPIRPGGNYTYKFQIKEQ 120

Query: 121 EGTLWWHAHSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 180
           EGTLWWHAHSSWLRATVHGALLIRPKS RPLPYPKPYKKIPILLGEWWNANVVHVEEEGL
Sbjct: 121 EGTLWWHAHSSWLRATVHGALLIRPKSNRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 180

Query: 181 ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH 240
           ATGSGPNISDAYTINGLPGNLYPCSQNQTY LKM RGKTYLLQVINAALNNQFFFK ANH
Sbjct: 181 ATGSGPNISDAYTINGLPGNLYPCSQNQTYQLKMVRGKTYLLQVINAALNNQFFFKFANH 240

Query: 241 NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT 300
           N TVVAVDA YTDPYVTDVIVLAPGQTTDVLVKADQP  SYYMAARPYADARPPIDFSDT
Sbjct: 241 NFTVVAVDATYTDPYVTDVIVLAPGQTTDVLVKADQPLGSYYMAARPYADARPPIDFSDT 300

Query: 301 ITRAIVVYNGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFV 360
           ITRAIV Y+GAS S AP+MP LP FNDTPTAHKFY+NITALVGARHW+PVPRHVD+HMFV
Sbjct: 301 ITRAIVTYDGASSSTAPVMPVLPAFNDTPTAHKFYSNITALVGARHWIPVPRHVDNHMFV 360

Query: 361 TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDF 420
           TFGLNLAPCG  N + CGGPNGQRLSASMNNVSFVIPNDAGLSMLEA+FHKV+GVYS DF
Sbjct: 361 TFGLNLAPCGTGNESTCGGPNGQRLSASMNNVSFVIPNDAGLSMLEAYFHKVEGVYSRDF 420

Query: 421 PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG 480
           PD PPV+FDYTN S+ LDNSLIFAPKAT VKKLKFNSTVEM+LQNTAF+SLENHPMHLHG
Sbjct: 421 PDDPPVKFDYTNPSLGLDNSLIFAPKATKVKKLKFNSTVEMILQNTAFISLENHPMHLHG 480

Query: 481 FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD 540
           FNFHVLAQGFGNYDPI DPKKFNF+NPQIRNTIAVPV GWAVIRFQANNPGVWLMHCHLD
Sbjct: 481 FNFHVLAQGFGNYDPIRDPKKFNFVNPQIRNTIAVPVGGWAVIRFQANNPGVWLMHCHLD 540

Query: 541 VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 574
           VHVPWGLAMGFEVENGPTPST LPPPPHDLPKC
Sbjct: 541 VHVPWGLAMGFEVENGPTPSTRLPPPPHDLPKC 571

BLAST of CmaCh03G003800.1 vs. NCBI nr
Match: XP_022950373.1 (LOW QUALITY PROTEIN: laccase-7-like [Cucurbita moschata])

HSP 1 Score: 1048.1 bits (2709), Expect = 2.7e-302
Identity = 503/573 (87.78%), Postives = 524/573 (91.45%), Query Frame = 0

Query: 1   MASSCLPLLLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH 60
           MASS   L LLLLLLLASSSMASAAIVEHSFTV+DMTVRRLCRDQ ITAVNGEYPGPTIH
Sbjct: 1   MASS---LALLLLLLLASSSMASAAIVEHSFTVEDMTVRRLCRDQVITAVNGEYPGPTIH 60

Query: 61  VHDGDILVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQ 120
           V D D+L+VHVSN SPYDLTIHWHGVFQLLS WADGPENVTQCPIRPGGNYTYRF IK Q
Sbjct: 61  VQDDDVLIVHVSNNSPYDLTIHWHGVFQLLSAWADGPENVTQCPIRPGGNYTYRFNIKGQ 120

Query: 121 EGTLWWHAHSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 180
           EGTLWWHAHSSWLRATVHGALLIRPKSG PLPY KPYKK+PILLGEWWNANVVHVEEEGL
Sbjct: 121 EGTLWWHAHSSWLRATVHGALLIRPKSGLPLPYQKPYKKVPILLGEWWNANVVHVEEEGL 180

Query: 181 ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH 240
           A G GPN SDAYTINGLPGNLYPC QNQTY LKMARGKT LLQV+NAALNNQ FFKLANH
Sbjct: 181 AAGRGPNGSDAYTINGLPGNLYPCPQNQTYQLKMARGKTSLLQVVNAALNNQLFFKLANH 240

Query: 241 NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT 300
           NLTVVAVDA YT PYVTDVIVLAPGQTTDVLV ADQP  SYYMAARPYAD RP I F +T
Sbjct: 241 NLTVVAVDATYTVPYVTDVIVLAPGQTTDVLVNADQPLGSYYMAARPYADTRPQIAFPNT 300

Query: 301 ITRAIVVYNGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFV 360
           ITRAIV+Y+GA  ++ P+MP LP FNDTPTAHKFYTN+TALVGARHW P PRHVDHHMFV
Sbjct: 301 ITRAIVIYDGAPTNSTPIMPLLPAFNDTPTAHKFYTNLTALVGARHWDPAPRHVDHHMFV 360

Query: 361 TFGLNLAPCGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDF 420
           TF LNLAPCG PNG  CGGPNGQRLSASM NVSFVIPNDAGLSMLEAFFHKV+GVY+ DF
Sbjct: 361 TFRLNLAPCGTPNG--CGGPNGQRLSASMXNVSFVIPNDAGLSMLEAFFHKVEGVYTGDF 420

Query: 421 PDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHG 480
           PD PPVEFDYTNSSIRLDNSLIFA KATSVKKLKFNSTVE+VLQNTAF++ ENHP+HLHG
Sbjct: 421 PDLPPVEFDYTNSSIRLDNSLIFAAKATSVKKLKFNSTVEIVLQNTAFIAKENHPLHLHG 480

Query: 481 FNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLD 540
           FNFHVLAQGFGNYDPIHDP+KFNFLNPQIRNTIAVP+ GW VIRFQANNPGVWLMHCHLD
Sbjct: 481 FNFHVLAQGFGNYDPIHDPEKFNFLNPQIRNTIAVPIGGWPVIRFQANNPGVWLMHCHLD 540

Query: 541 VHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 574
           VH+PWGLAMGFEV NGPTPST LPPPPHDLPKC
Sbjct: 541 VHLPWGLAMGFEVANGPTPSTRLPPPPHDLPKC 568

BLAST of CmaCh03G003800.1 vs. TAIR 10
Match: AT3G09220.1 (laccase 7 )

HSP 1 Score: 738.0 bits (1904), Expect = 5.6e-213
Identity = 357/566 (63.07%), Postives = 432/566 (76.33%), Query Frame = 0

Query: 13  LLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHVHDGDILVVHVS 72
           L+LLA SS+ SA+IVEH+F VQ++TV RLC+ Q IT VNG  PGPTI V +GD LV+HV 
Sbjct: 12  LILLAISSITSASIVEHTFNVQNLTVSRLCKRQVITVVNGSLPGPTIRVKEGDSLVIHVL 71

Query: 73  NKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAHSSW 132
           N SP+++TIHWHG+F  L+ WADGP  +TQCPI+PG  Y YRF I  QEGTLWWHAH+S+
Sbjct: 72  NHSPHNITIHWHGIFHKLTVWADGPSMITQCPIQPGQRYAYRFNITGQEGTLWWHAHASF 131

Query: 133 LRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNISDAY 192
           LRATV+GAL+IRPKSG   P+PKP+K++PIL GEWWN +VV +EE  +ATG  PN SDAY
Sbjct: 132 LRATVYGALVIRPKSGHSYPFPKPHKEVPILFGEWWNTDVVALEEAAIATGVPPNNSDAY 191

Query: 193 TINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYT 252
           TING PGNLYPCS+++ ++L + +GK YLL++INAA+N Q FFK+ANH LTVVA DAVYT
Sbjct: 192 TINGRPGNLYPCSKDRMFSLNVVKGKRYLLRIINAAMNIQLFFKIANHRLTVVAADAVYT 251

Query: 253 DPYVTDVIVLAPGQTTDVLVKADQP-PRSYYMAARPYADARPPIDFSDTITRAIVVYNGA 312
            PYVTDVIV+APGQT D L+ ADQ    SYYMAA PYA A P + F +T TR ++ Y GA
Sbjct: 252 APYVTDVIVIAPGQTIDALLFADQSVDTSYYMAAHPYASA-PAVPFPNTTTRGVIHYGGA 311

Query: 313 SP---SAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFVTFGLNLAP 372
           S    S   LMP LP F DT TA++FY+N+TALV   HWVPVPR+VD  M VT GL L  
Sbjct: 312 SKTGRSKPVLMPKLPSFFDTLTAYRFYSNLTALVNGPHWVPVPRYVDEEMLVTIGLGLEA 371

Query: 373 CGLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDFPDQPPVEF 432
           C   + T C      + SASM+N SFV+P    LS+LEA FH VKG+++ DFPDQPPV+F
Sbjct: 372 CA--DNTTC-----PKFSASMSNHSFVLPKK--LSILEAVFHDVKGIFTADFPDQPPVKF 431

Query: 433 DYTNSSIRLDN-SLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLA 492
           DYTN ++   N  L+F  K+TS K LKFN+TVE+VLQN A ++ E+HPMHLHGFNFHVLA
Sbjct: 432 DYTNPNVTQTNPGLLFTQKSTSAKILKFNTTVEVVLQNHALIAAESHPMHLHGFNFHVLA 491

Query: 493 QGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGL 552
           QGFGNYDP  D  K N ++PQ RNT+AVPV GWAVIRF ANNPG W+ HCH+DVH+P+GL
Sbjct: 492 QGFGNYDPSRDRSKLNLVDPQSRNTLAVPVGGWAVIRFTANNPGAWIFHCHIDVHLPFGL 551

Query: 553 AMGFEVENGPTPSTWLPPPPHDLPKC 574
            M F V+NGPT ST LPPPP DLPKC
Sbjct: 552 GMIFVVKNGPTKSTTLPPPPPDLPKC 567

BLAST of CmaCh03G003800.1 vs. TAIR 10
Match: AT5G01050.1 (Laccase/Diphenol oxidase family protein )

HSP 1 Score: 681.8 bits (1758), Expect = 4.8e-196
Identity = 323/565 (57.17%), Postives = 410/565 (72.57%), Query Frame = 0

Query: 13  LLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHVHDGDILVVHVS 72
           L+LL  SS+ASAAIVEH   V+D+ V  LC++Q I  VNG  PGPTI+V +GD LVVHV 
Sbjct: 14  LVLLLFSSIASAAIVEHVLHVKDVVVTPLCKEQMIPIVNGSLPGPTINVREGDTLVVHVI 73

Query: 73  NKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAHSSW 132
           NKS Y++TIHWHGVFQL S W DG   +TQCPI+P  N+TY+F I  QEGTL WHAH   
Sbjct: 74  NKSTYNVTIHWHGVFQLKSVWMDGANMITQCPIQPSNNFTYQFDITGQEGTLLWHAHVVN 133

Query: 133 LRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNISDAY 192
           LRAT+HGAL+IRP+SGRP P+PKPYK++P++  +WW+ +V  +E           +SDAY
Sbjct: 134 LRATIHGALIIRPRSGRPYPFPKPYKEVPLIFQQWWDTDVRLLELRPAP------VSDAY 193

Query: 193 TINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYT 252
            INGL G+ YPCS+N+ + LK+ +GKTYLL++INAALN   FFK+ANHN+TVVAVDAVYT
Sbjct: 194 LINGLAGDSYPCSKNRMFNLKVVQGKTYLLRIINAALNTHLFFKIANHNVTVVAVDAVYT 253

Query: 253 DPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADA-RPPIDFSDTITRAIVVYNGA 312
            PY+TDV++L PGQT D ++ ADQP  +YYMA  PY  A   P       TR ++VY GA
Sbjct: 254 TPYLTDVMILTPGQTIDAILTADQPIGTYYMAIIPYFSAIGVPASPDTKPTRGLIVYEGA 313

Query: 313 SPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFVTFGLNLAPCGL 372
           + S++P  P +PP ND PTAH+F +NIT+LVG  HW PVPRHVD  MF+T GL L PC  
Sbjct: 314 TSSSSPTKPWMPPANDIPTAHRFSSNITSLVGGPHWTPVPRHVDEKMFITMGLGLDPC-- 373

Query: 373 PNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDFPDQPPVEFDYT 432
           P+   C GP  QRL+ S+NN +F+IP    +SM EA+F+ + GVY+ DFPDQPP++FD+T
Sbjct: 374 PSNAKCVGPLDQRLAGSLNNRTFMIPE--RISMQEAYFYNITGVYTDDFPDQPPLKFDFT 433

Query: 433 NSSIRLDNS---LIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLAQ 492
                  NS   ++F  + TSVK ++FNSTVE+VLQNT  ++ E+HPMHLHGFNF+VL  
Sbjct: 434 KFEQHPTNSDMEMMFPERKTSVKTIRFNSTVEIVLQNTGILTPESHPMHLHGFNFYVLGY 493

Query: 493 GFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGLA 552
           GFGNYDPI D +K N  NPQ+ NT+ VP  GW V+RF ANNPG+WL HCH+D H+P G+ 
Sbjct: 494 GFGNYDPIRDARKLNLFNPQMHNTVGVPPGGWVVLRFIANNPGIWLFHCHMDAHLPLGIM 553

Query: 553 MGFEVENGPTPSTWLPPPPHDLPKC 574
           M F V+NGPT  T LP PP +LP+C
Sbjct: 554 MAFIVQNGPTRETSLPSPPSNLPQC 568

BLAST of CmaCh03G003800.1 vs. TAIR 10
Match: AT5G01040.1 (laccase 8 )

HSP 1 Score: 681.4 bits (1757), Expect = 6.3e-196
Identity = 325/565 (57.52%), Postives = 413/565 (73.10%), Query Frame = 0

Query: 13  LLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHVHDGDILVVHVS 72
           L+LL  SS+ASAA+VEH   +QD+ V+ LC++Q I A NG  PGPTI+V +GD LVV+V 
Sbjct: 14  LVLLLFSSIASAAVVEHVLHIQDVVVKPLCKEQIIPAANGSLPGPTINVREGDTLVVNVI 73

Query: 73  NKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAHSSW 132
           N S Y++TIHWHGVFQL S W DG   +TQCPI+PG N+TY+F I  QEGTL WHAH   
Sbjct: 74  NNSTYNVTIHWHGVFQLKSVWMDGANMITQCPIQPGYNFTYQFDITGQEGTLLWHAHVVN 133

Query: 133 LRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNISDAY 192
           LRAT+HGAL+IRP+SGRP P+PKPYK++PI+  +WW+ +V       L     P +SDAY
Sbjct: 134 LRATLHGALVIRPRSGRPYPFPKPYKEVPIVFQQWWDTDV-----RLLQLRPAP-VSDAY 193

Query: 193 TINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVDAVYT 252
            INGL G+ YPCS+N+ + LK+ +GKTYLL+++NAALN   FFK+ANHN+TVVAVDAVY+
Sbjct: 194 LINGLAGDSYPCSENRMFNLKVVQGKTYLLRIVNAALNTHLFFKIANHNVTVVAVDAVYS 253

Query: 253 DPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADA--RPPIDFSDTITRAIVVYNG 312
            PY+TDV++L PGQT D L+ ADQ    YYMA  PY  A   P  D     TR ++VY G
Sbjct: 254 TPYLTDVMILTPGQTVDALLTADQAIGKYYMATLPYISAIGIPTPDIKP--TRGLIVYQG 313

Query: 313 ASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFVTFGLNLAPCG 372
           A+ S++P  P +P  ND  TAH+F +NIT+LVG  HW PVPRHVD  MF+T GL L PC 
Sbjct: 314 ATSSSSPAEPLMPVPNDMSTAHRFTSNITSLVGGPHWTPVPRHVDEKMFITMGLGLDPC- 373

Query: 373 LPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDFPDQPPVEFDY 432
            P GT C GP GQR + S+NN +F+IP    +SM EA+F+ + G+Y+ DFP+QPP++FDY
Sbjct: 374 -PAGTKCIGPLGQRYAGSLNNRTFMIPE--RISMQEAYFYNISGIYTDDFPNQPPLKFDY 433

Query: 433 TNSSIRLDN--SLIFAPKATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHVLAQ 492
           T    R +N   ++F  + TSVKK++FNSTVE+VLQNTA +S E+HPMHLHGFNF+VL  
Sbjct: 434 TKFEQRTNNDMKMMFPERKTSVKKIRFNSTVEIVLQNTAIISPESHPMHLHGFNFYVLGY 493

Query: 493 GFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPWGLA 552
           GFGNYDPI D +K N  NPQ+ NT+ VP  GW V+RF ANNPGVWL HCH+D H+P+G+ 
Sbjct: 494 GFGNYDPIRDARKLNLFNPQMHNTVGVPPGGWVVLRFIANNPGVWLFHCHMDAHLPYGIM 553

Query: 553 MGFEVENGPTPSTWLPPPPHDLPKC 574
             F V+NGPTP T LP PP +LP+C
Sbjct: 554 SAFIVQNGPTPETSLPSPPSNLPQC 566

BLAST of CmaCh03G003800.1 vs. TAIR 10
Match: AT5G05390.1 (laccase 12 )

HSP 1 Score: 620.5 bits (1599), Expect = 1.3e-177
Identity = 303/568 (53.35%), Postives = 397/568 (69.89%), Query Frame = 0

Query: 9   LLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIHVHDGDILV 68
           +LL    L S+S+  A +  H F +Q+  V+RLC+ +    VNG +PGPT+ V++GD L 
Sbjct: 9   ILLFFCSLFSASLIIAKVQHHDFVIQETPVKRLCKTRNAITVNGMFPGPTLEVNNGDTLE 68

Query: 69  VHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHA 128
           V V N++ Y++TIHWHGV Q+ +GWADGPE VTQCPIRPG +YTYRF I+ QEGTLWWHA
Sbjct: 69  VKVHNRARYNITIHWHGVRQIRTGWADGPEFVTQCPIRPGKSYTYRFTIQGQEGTLWWHA 128

Query: 129 HSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGLATGSGPNI 188
           HSSWLRATV+GAL+I P  G   P+PKP ++  ++LGEWWNAN V V  +   TG+ PNI
Sbjct: 129 HSSWLRATVYGALIIHPTPGSSFPFPKPDRQTALMLGEWWNANPVDVINQATRTGAAPNI 188

Query: 189 SDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNLTVVAVD 248
           SDAYTING PG+LY CS  +T  + +  G+T LL+VINAALN   FF +ANH LTVV  D
Sbjct: 189 SDAYTINGQPGDLYNCSTKETVVVPINSGETSLLRVINAALNQPLFFTVANHKLTVVGAD 248

Query: 249 AVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDTITRAIVVY 308
           A Y  P+ T V++L PGQTTDVL+ ADQPP+ YY+AAR Y  A+    F +T T AI+ Y
Sbjct: 249 ASYLKPFTTKVLMLGPGQTTDVLLTADQPPKRYYIAARAYQSAQ-NAPFDNTTTTAILQY 308

Query: 309 NGASPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHWVPVPRHVDHHMFVTFGLNLAP 368
              + ++ P+MP LP FNDT T   F     +L      V VP+ +D ++F T GL L  
Sbjct: 309 KKTTTTSKPIMPVLPAFNDTNTVTSFSRKFKSLRN----VVVPKTIDDNLFFTIGLGLDN 368

Query: 369 C--GLPNGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLEAFFHKVKGVYSTDFPDQPPV 428
           C    P  + C G NG R +ASMNNVSFV+P++   S+L+A  + + GV++TDFP +PPV
Sbjct: 369 CPKKFPK-SRCQGLNGTRFTASMNNVSFVLPSN--FSLLQAHSNGIPGVFTTDFPSKPPV 428

Query: 429 EFDYTNSSIRLDNSLIFAP-KATSVKKLKFNSTVEMVLQNTAFVSLENHPMHLHGFNFHV 488
           +FDYT ++I   +  +F P K T + KLK+ S V++VLQ+T  V+ ENHP+HLHG++F++
Sbjct: 429 KFDYTGNNI---SRALFQPVKGTKLYKLKYGSRVQVVLQDTNIVTSENHPIHLHGYDFYI 488

Query: 489 LAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQANNPGVWLMHCHLDVHVPW 548
           + +GFGN++P  D  KFN ++P +RNT+AVPV GWAVIRF A+NPGVWLMHCHLDVH+ W
Sbjct: 489 VGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGVWLMHCHLDVHIKW 548

Query: 549 GLAMGFEVENGPTPSTWLPPPPHDLPKC 574
           GLAM F V+NG      L  PPHDLP C
Sbjct: 549 GLAMAFLVDNGVGELETLEAPPHDLPIC 565

BLAST of CmaCh03G003800.1 vs. TAIR 10
Match: AT2G40370.1 (laccase 5 )

HSP 1 Score: 604.0 bits (1556), Expect = 1.3e-172
Identity = 302/587 (51.45%), Postives = 388/587 (66.10%), Query Frame = 0

Query: 1   MASSCLPLLLLLLLLLASSSMASAAIVEHSFTVQDMTVRRLCRDQEITAVNGEYPGPTIH 60
           +  S L  +  +  LL SS   +     H F +Q   V+RLC       VNG +PGP + 
Sbjct: 3   VTKSLLCFISFVAFLLFSSVAEANKAHHHEFIIQATKVKRLCETHNSITVNGMFPGPMLV 62

Query: 61  VHDGDILVVHVSNKSPYDLTIHWHGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQ 120
           V++GD LVV V N++ Y++TIHWHGV Q+ +GWADGPE VTQCPIRPG +YTYRF I+ Q
Sbjct: 63  VNNGDTLVVKVINRARYNITIHWHGVRQMRTGWADGPEFVTQCPIRPGSSYTYRFTIQGQ 122

Query: 121 EGTLWWHAHSSWLRATVHGALLIRPKSGRPLPYPKPYKKIPILLGEWWNANVVHVEEEGL 180
           EGTLWWHAHSSWLRATV+G+LL+ P +G   P+ KP++ +P+LLGEWW+AN V V  E +
Sbjct: 123 EGTLWWHAHSSWLRATVYGSLLVFPPAGSSYPFTKPHRNVPLLLGEWWDANPVDVLRESI 182

Query: 181 ATGSGPNISDAYTINGLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANH 240
            TG  PN SDAYTING PG+LY CS   T  + +  G+T LL+VIN+ALN   FF +ANH
Sbjct: 183 RTGGAPNNSDAYTINGQPGDLYKCSSQDTTVVPINVGETILLRVINSALNQPLFFTVANH 242

Query: 241 NLTVVAVDAVYTDPYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPYADARPPIDFSDT 300
            LTVV  DA Y  P+ T+VIVL PGQTTDVL+  DQPP  YYMAAR Y  A+    F +T
Sbjct: 243 KLTVVGADASYLKPFTTNVIVLGPGQTTDVLITGDQPPNRYYMAARAYQSAQ-NAPFGNT 302

Query: 301 ITRAIVVYNGA-------------SPSAAPLMPTLPPFNDTPTAHKFYTNITALVGARHW 360
            T AI+ Y  A               S  P+MP LP +NDT T  +F  +  +L  A   
Sbjct: 303 TTTAILQYKSAPCCGVGGGSGTKKGNSFKPIMPILPAYNDTNTVTRFSQSFRSLRRAE-- 362

Query: 361 VPVPRHVDHHMFVTFGLNLAPCGLP-NGTACGGPNGQRLSASMNNVSFVIPNDAGLSMLE 420
             VP  +D ++FVT GL L  C        C GPNG R +ASMNNVSF +P++   S+L+
Sbjct: 363 --VPTEIDENLFVTIGLGLNNCPKNFRSRRCQGPNGTRFTASMNNVSFALPSN--YSLLQ 422

Query: 421 AFFHKVKGVYSTDFPDQPPVEFDYTNSSIRLDNSLIFAPKATSVKKLKFNSTVEMVLQNT 480
           A  H + GV++TDFP +PPV+FDYT ++I    SL    + T + KLK+ S V++VLQ+T
Sbjct: 423 AHHHGIPGVFTTDFPAKPPVKFDYTGNNI--SRSLYQPDRGTKLYKLKYGSRVQIVLQDT 482

Query: 481 AFVSLENHPMHLHGFNFHVLAQGFGNYDPIHDPKKFNFLNPQIRNTIAVPVAGWAVIRFQ 540
             V+ ENHP+HLHG++F+++A+GFGN++P  D  KFN  +P +RNT+ VPV GWAVIRF 
Sbjct: 483 GIVTPENHPIHLHGYDFYIIAEGFGNFNPKKDTAKFNLEDPPLRNTVGVPVNGWAVIRFI 542

Query: 541 ANNPGVWLMHCHLDVHVPWGLAMGFEVENGPTPSTWLPPPPHDLPKC 574
           A+NPGVW+MHCHLD H+ WGLAM F VENG      +  PPHDLP C
Sbjct: 543 ADNPGVWIMHCHLDAHISWGLAMAFLVENGNGVLQTIEQPPHDLPVC 580