CmUC02G040630.1 (mRNA) Watermelon (USVL531) v1

Overview


Name	CmUC02G040630.1
Type	mRNA
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	RNA pseudouridine synthase 1
Location	CmU531Chr02: 28419010 .. 28421425 (+)
Sequence length	1248
RNA-Seq Expression	CmUC02G040630.1
Synteny	CmUC02G040630.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCCTCTTCACTGCACTTCAGCCCACTTTCTCAATTTCCCACAAAACCCTCACCAAATTCCCAATCCCAAAACCCTTCTGTTTCCATAGATTCAAATCCGTCAGCAATAGCATGGCGGACTCATCGGAAAAGGTGGCCAGAAACTGCCCGTCAACCGGAGAGAATTACCCGGTGCCCTTATCGCCTCCACTGGCGGCCATATCCAAGAACTTGGAGCTCGCCAGAGCTATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAGGACGAGTGGCTTATTGCCGTCAATAAGCCTCAAGGAATCTACTGCGAAAGCGTTTTGGCCGCTGTTCCTCGTCTTCTCGGTGATTCGGCCATGGCTGGTTTGTGGATCTTACTGTGAAAGCGTTTTTCTTTTCAGACGAATTCGAATTTTAACTTGAACTTGGCAAGATGAATTCAATTTTACCCTATATTTTGAATTTGCTAATCGAATGGAAATGGTGGAACTGCAAGTTTTAACTTGCTCCTGTTTGAGGATTTATAAAAAGGTATAATCGCGTGTTTTCCATGGATTACAAAATGTAGACGACGCTGCAAAGGAAGAAGATATTAGGGCTGAATTTTTGTTGAAATTGACGAGTTTTTGAAAGATTTATTCATTATAGATCGTGTGCATTGATTTTCTAGATCATACTTTTGTACATTGATATGTAACTTGGTCTTGGTTGAAATTAGCTGAAATTAGTGGATAAATGTAATAGGAGTCTAACAGAAGTTTTAAAAATTGTGATGCTTACCTACGTGTAAGGTTCATTGTGATGAAATTTTGAAGTTTGGAGTAATAATTGACTCACCTTGCTAAGTTTTGGGTAGGATTTAATCTTTCATCATATTTGTTTACAAGGAATGATTTTCTGGAATTTGTTTTCTATTGTTTTAAGCTGCTTAAAAATGCAATTTCCCAAGTTCTATTTCTATTTCTACTTTTCTTTTTGAGAAACCTCGGTGTGAGGACGGTTTGGTCTTGTATTTGGTTCAACGTGTCTTTGTGGGCGTCTGTAATTAACCTTTTTGAAATTGTTCGTTTGGTCTTATATTGCTAAATTTATTTTCACTTTTGTGGGCTCCCTTTTTGTCTGCCCTTAATTCTTTTTACCTTTTTTTCTCAAATGAAAGTTCGGTTATTCTTTAAAAAAAGACAAAAATGATCAAAATTATCGATCCTTTTTCGTTTTGATGAAACTCATAGTCTAATGGTTTTTCAAGAGAGAGACAAAAATCTATCTCTACCGTTTTTTAGAATGAGCATTATTTTTAACTTAACTGTCAAAGAAGGCTATAAATTATGTCATCAAATTATATGGTATTCAAGTTTTCTGGGGTGCTTTGTGGTATATAGATATCATGATAGGTGGGCATGCTCTGGTAGTTTGATATGAATACATTTACTTATAAGCGAGAGCGAGTAATTGATTTCTTTTTCTTTCCCTGCATTGCCAGAACTAGCCATGTTGCTGATTGTTAGACAGTAAATATAAATGGTTTAGATGTATCCTGATTGTTGAAGAGAGAGATGTTTGAATTTGTCTGAAGTGTTTTAACGTTTGTCTAAAACAATGCCATGTAATTACATCTTTTGCTTATTCTATCATTATCGAGTTCTTGGGAGTGTTCTTTTCTCAGATTCTTTATATGCAGGGATCAAAACCAGTCTGCCAGAGCTTCATCTTGCTAACCGCCTCGATCGTGACACAAGTGGGGTTATGGTTATAACTAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACACAGTTTCAAAATCATATATCGCCTTCTGTGTTGGCTCTACTCCAAAGTGGAAAAAAATCAACGTCAAATCTGGTCATGGAAGGTCGAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCGTAGTCAGAGACATGGAAACATATTTTGAAGTACTATCTGTAAATGGAAAAAATGTCATGGAGGGATTTCAAAAATTTAGGAAACATGAAGAAGAAACTATTGTAGTCCAAACAAAATCTCTGGTGGAAATTGATGGTCAAAAGGACGAGATCTTGATAAGAGCACGACCTTGCAGTGGAAGAACTCATCAAATTCGTCTGCACTGCCAATATCTTGGAATTCCAATAAGAGGGGATGTGAAATATGAGGGTGTTACTGAGTGGAATGGAAAAGTTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTGTTACAGGTATTCCTCTCAAACTACAAGCTCCTCTACCATCATGGGCCAGCCAAGCATTGCAGCCCCAACAAGAAGTGAGTTCTCCAACATCCTTCAAACCTATGCCTTAA

mRNA sequence

ATGGCCCTCTTCACTGCACTTCAGCCCACTTTCTCAATTTCCCACAAAACCCTCACCAAATTCCCAATCCCAAAACCCTTCTGTTTCCATAGATTCAAATCCGTCAGCAATAGCATGGCGGACTCATCGGAAAAGGTGGCCAGAAACTGCCCGTCAACCGGAGAGAATTACCCGGTGCCCTTATCGCCTCCACTGGCGGCCATATCCAAGAACTTGGAGCTCGCCAGAGCTATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAGGACGAGTGGCTTATTGCCGTCAATAAGCCTCAAGGAATCTACTGCGAAAGCGTTTTGGCCGCTGTTCCTCGTCTTCTCGGTGATTCGGCCATGGCTGGTTTGTGGATCTTACTGTATAATCGCGTGTTTTCCATGGATTACAAAATATCGTCTGAAATTAGTGGATATCATGATAGGTGGGCATGCTCTGAACTAGCCATGTTGCTGATTGTTAGACATGTTCTTTTCTCAGATTCTTTATATGCAGGGATCAAAACCAGTCTGCCAGAGCTTCATCTTGCTAACCGCCTCGATCGTGACACAAGTGGGGTTATGGTTATAACTAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACACAGTTTCAAAATCATATATCGCCTTCTGTGTTGGCTCTACTCCAAAGTGGAAAAAAATCAACGTCAAATCTGGTCATGGAAGGTCGAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCGTAGTCAGAGACATGGAAACATATTTTGAAGTACTATCTGTAAATGGAAAAAATGTCATGGAGGGATTTCAAAAATTTAGGAAACATGAAGAAGAAACTATTGTAGTCCAAACAAAATCTCTGGTGGAAATTGATGGTCAAAAGGACGAGATCTTGATAAGAGCACGACCTTGCAGTGGAAGAACTCATCAAATTCGTCTGCACTGCCAATATCTTGGAATTCCAATAAGAGGGGATGTGAAATATGAGGGTGTTACTGAGTGGAATGGAAAAGTTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTGTTACAGGTATTCCTCTCAAACTACAAGCTCCTCTACCATCATGGGCCAGCCAAGCATTGCAGCCCCAACAAGAAGTGAGTTCTCCAACATCCTTCAAACCTATGCCTTAA

Coding sequence (CDS)

ATGGCCCTCTTCACTGCACTTCAGCCCACTTTCTCAATTTCCCACAAAACCCTCACCAAATTCCCAATCCCAAAACCCTTCTGTTTCCATAGATTCAAATCCGTCAGCAATAGCATGGCGGACTCATCGGAAAAGGTGGCCAGAAACTGCCCGTCAACCGGAGAGAATTACCCGGTGCCCTTATCGCCTCCACTGGCGGCCATATCCAAGAACTTGGAGCTCGCCAGAGCTATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAGGACGAGTGGCTTATTGCCGTCAATAAGCCTCAAGGAATCTACTGCGAAAGCGTTTTGGCCGCTGTTCCTCGTCTTCTCGGTGATTCGGCCATGGCTGGTTTGTGGATCTTACTGTATAATCGCGTGTTTTCCATGGATTACAAAATATCGTCTGAAATTAGTGGATATCATGATAGGTGGGCATGCTCTGAACTAGCCATGTTGCTGATTGTTAGACATGTTCTTTTCTCAGATTCTTTATATGCAGGGATCAAAACCAGTCTGCCAGAGCTTCATCTTGCTAACCGCCTCGATCGTGACACAAGTGGGGTTATGGTTATAACTAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACACAGTTTCAAAATCATATATCGCCTTCTGTGTTGGCTCTACTCCAAAGTGGAAAAAAATCAACGTCAAATCTGGTCATGGAAGGTCGAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCGTAGTCAGAGACATGGAAACATATTTTGAAGTACTATCTGTAAATGGAAAAAATGTCATGGAGGGATTTCAAAAATTTAGGAAACATGAAGAAGAAACTATTGTAGTCCAAACAAAATCTCTGGTGGAAATTGATGGTCAAAAGGACGAGATCTTGATAAGAGCACGACCTTGCAGTGGAAGAACTCATCAAATTCGTCTGCACTGCCAATATCTTGGAATTCCAATAAGAGGGGATGTGAAATATGAGGGTGTTACTGAGTGGAATGGAAAAGTTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTGTTACAGGTATTCCTCTCAAACTACAAGCTCCTCTACCATCATGGGCCAGCCAAGCATTGCAGCCCCAACAAGAAGTGAGTTCTCCAACATCCTTCAAACCTATGCCTTAA

Protein sequence

MALFTALQPTFSISHKTLTKFPIPKPFCFHRFKSVSNSMADSSEKVARNCPSTGENYPVPLSPPLAAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWACSELAMLLIVRHVLFSDSLYAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSTPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNVMEGFQKFRKHEEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQEVSSPTSFKPMP

Homology

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BLAST of CmUC02G040630.1 vs. NCBI nr
Match: XP_004144814.3 (RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein Csa_021313 [Cucumis sativus])

HSP 1 Score: 627.9 bits (1618), Expect = 6.4e-176
Identity = 328/416 (78.85%), Postives = 338/416 (81.25%), Query Frame = 0


Query: 1   MALFTALQPTFSISHKTLTKFPIPKPFCFHRFKSVSNSMADSSEKVARNCPSTGENYPVP 60
           MALF +L P FSIS KT T+FP  K FCFHRFKSVSNSM DSSE V  NCPST ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLAAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120
           LSPPL AISKNLELARAM ASSKSSL+ALSANDVIYEDEWLIAVNKPQGIYCE+VLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWACSELAMLLIVRHVLFSDSLYAG 180
           RLLGDSA                                                   AG
Sbjct: 121 RLLGDSA--------------------------------------------------NAG 180

Query: 181 IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSTPKWKK 240
           IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVG++PKWKK
Sbjct: 181 IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKK 240

Query: 241 INVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNVMEGFQKFRKH 300
           INVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKN ME  QKFR+ 
Sbjct: 241 INVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNTMEELQKFRRG 300

Query: 301 EEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWN 360
           EEETIVV TKSLV+ID  KDEILIRARP SGRTHQIRLHCQYLGIPIRGDVKYEGVTEWN
Sbjct: 301 EEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWN 360

Query: 361 GKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQ-EVSSPTSFKPMP 416
            K+YDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQ EV+SP SFK MP
Sbjct: 361 EKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHEVNSPQSFKTMP 366

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BLAST of CmUC02G040630.1 vs. NCBI nr
Match: XP_038889468.1 (RNA pseudouridine synthase 1 [Benincasa hispida])

HSP 1 Score: 612.5 bits (1578), Expect = 2.8e-171
Identity = 322/409 (78.73%), Postives = 334/409 (81.66%), Query Frame = 0


Query: 1   MALFTALQPTFSISHKTLTKFPIPKPFCFHRFKSVSNSMADSSEKVARNCPSTGENYPVP 60
           MAL  +LQPTFS  HKT T+F  PKPFC  RFKS+S SMADSSE V  NCPST ENYPVP
Sbjct: 1   MALLISLQPTFSTPHKTFTEF--PKPFCSGRFKSISKSMADSSEPVTGNCPSTRENYPVP 60

Query: 61  LSPPLAAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120
           LSPPL AISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120

Query: 121 RLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWACSELAMLLIVRHVLFSDSLYAG 180
           RLL DSAM                                                  AG
Sbjct: 121 RLLSDSAM--------------------------------------------------AG 180

Query: 181 IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSTPKWKK 240
           IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGS PKWKK
Sbjct: 181 IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKK 240

Query: 241 INVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNVMEGFQKFRKH 300
           IN+KSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLS+NG+N ME FQKF K 
Sbjct: 241 INIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSINGQNAMEEFQKFGK- 300

Query: 301 EEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWN 360
           +EET+VVQTKSLV+IDGQKDEILIRARP SGRTHQIRLHCQYLGIPIRGDVKYEGVTEWN
Sbjct: 301 DEETVVVQTKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWN 356

Query: 361 GKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQP-QQEVSSP 409
           GK+YD HELHAESLYFVHP+TGIPLKLQAPLPSWASQA QP QQEV+SP
Sbjct: 361 GKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWASQAWQPQQQEVNSP 356

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BLAST of CmUC02G040630.1 vs. NCBI nr
Match: XP_008453651.1 (PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa] >TYJ96474.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 576.2 bits (1484), Expect = 2.2e-160
Identity = 300/378 (79.37%), Postives = 310/378 (82.01%), Query Frame = 0


Query: 39  MADSSEKVARNCPSTGENYPVPLSPPLAAISKNLELARAMAASSKSSLFALSANDVIYED 98
           M DSSE V  NCPSTGENYPVPLSPPL AISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWA 158
           EWLIAVNKPQGIYCE+VLAAVPRLL DSA                               
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSA------------------------------- 120

Query: 159 CSELAMLLIVRHVLFSDSLYAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAF 218
                               AGIKT+LPELHLANRLDRDTSGVMVITKSHKVASKLVKAF
Sbjct: 121 -------------------NAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAF 180

Query: 219 TDHTVSKSYIAFCVGSTPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET 278
           TDHTVSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMET
Sbjct: 181 TDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMET 240

Query: 279 YFEVLSVNGKNVMEGFQKFRKHEEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIRL 338
           YFEVLSVNGKN ME  QKFRK EEETIVV TKSLV+ID QKDE+LIRARP SGRTHQIRL
Sbjct: 241 YFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRL 300

Query: 339 HCQYLGIPIRGDVKYEGVTEWNGKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQA 398
           HCQYLGIPIRGDVKYEGVTEWNGK YDSHELHAESLYFVHP+TGIPLKLQAPLPSWASQA
Sbjct: 301 HCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQA 328

Query: 399 LQP-QQEVSSPTSFKPMP 416
           LQP QQEV+SP SFK MP
Sbjct: 361 LQPQQQEVNSPQSFKTMP 328

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BLAST of CmUC02G040630.1 vs. NCBI nr
Match: KAG7021056.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 565.8 bits (1457), Expect = 3.0e-157
Identity = 306/420 (72.86%), Postives = 324/420 (77.14%), Query Frame = 0


Query: 1   MALFTALQPTFSISHKTLTKFPIPKPFCFHRFKSVSNSMADSSEKVARNCPSTGENYPVP 60
           MAL   +QP FSI HK+  KF  PKPF FHRF+ V+NSMADSS+      P TGENYPVP
Sbjct: 6   MALVVTIQPPFSIPHKSFAKF--PKPFRFHRFRPVTNSMADSSK------PVTGENYPVP 65

Query: 61  LSPPLAAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120
           LSPPL AISKNLELARAMAA SKSSLF+LSA DVIYEDEWLIAVNKPQG+YCESVLA+VP
Sbjct: 66  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKPQGVYCESVLASVP 125

Query: 121 RLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWACSELAMLLIVRHVLFSDSLYAG 180
           R LGDSAMAG                                             +    
Sbjct: 126 RFLGDSAMAG---------------------------------------------AQSGE 185

Query: 181 IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSTPKWKK 240
           IK SLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSKSYIAFCVGS PKWKK
Sbjct: 186 IKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKK 245

Query: 241 INVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNVMEGFQKFRK- 300
           I VKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET FEVLSVNG+N  E   + RK 
Sbjct: 246 IIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSVNGQNTKEELPESRKD 305

Query: 301 ---HEEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIRLHCQYLGIPIRGDVKYEGV 360
               EEETIV Q+KSL++ID +KDEILIRARP SGRTHQIRLH QYLGIPI GDVKYEGV
Sbjct: 306 EEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQYLGIPIVGDVKYEGV 365

Query: 361 TEWNGKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQP-QQEVSSPTSFKPMP 416
           TEWNGK+YDSHELHAESLYFVHPVTGIPLKLQAPLPSWA+QALQP QQEV+S  S KPMP
Sbjct: 366 TEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQALQPQQQEVNSAASLKPMP 372

[Close]

BLAST of CmUC02G040630.1 vs. NCBI nr
Match: KAG6586224.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 558.9 bits (1439), Expect = 3.6e-155
Identity = 306/422 (72.51%), Postives = 324/422 (76.78%), Query Frame = 0


Query: 1   MALFTALQPTFSISHKTLTKFPIPKPFCFHRFKSVSNSMADSSEKVARNCPSTGENYPVP 60
           MAL   LQ  FSI +K+  KF  PKPF FHRF+ V+NSMADSS+      P TGENYPVP
Sbjct: 1   MALVVTLQAPFSIPNKSFAKF--PKPFRFHRFRPVTNSMADSSK------PVTGENYPVP 60

Query: 61  LSPPLAAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120
           LSPPL AISKNLELARAMAA SKSSLF+LSA DV+YEDEWLIAVNKPQG+YCESVLA+VP
Sbjct: 61  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVVYEDEWLIAVNKPQGVYCESVLASVP 120

Query: 121 RLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWACSELAMLLIVRHVLFSDSLYAG 180
             LGDSAMAG                  E SG                            
Sbjct: 121 CFLGDSAMAG------------------EQSG---------------------------E 180

Query: 181 IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSTPKWKK 240
           IK SLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSKSYIAFCVGS PKWKK
Sbjct: 181 IKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKK 240

Query: 241 INVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNVMEGFQKFRK- 300
           I VKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET FEVLSVNG+N  E   + RK 
Sbjct: 241 IIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSVNGQNTKEELPESRKD 300

Query: 301 -----HEEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIRLHCQYLGIPIRGDVKYE 360
                 EEETIV Q+KSL++ID +KDEILIRARP SGRTHQIRLH QYLGIPI GDVKYE
Sbjct: 301 EEEEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQYLGIPIVGDVKYE 360

Query: 361 GVTEWNGKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQP-QQEVSSPTSFKP 416
           GVTEWNGK+YDSHELHAESLYFVHPVTGIPLKLQAPLPSWA+QALQP QQEV+S  S KP
Sbjct: 361 GVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQALQPQQQEVNSAASLKP 369

[Close]

BLAST of CmUC02G040630.1 vs. ExPASy Swiss-Prot
Match: Q2QNM3 (RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0560500 PE=2 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.2e-92
Identity = 183/350 (52.29%), Postives = 224/350 (64.00%), Query Frame = 0


Query: 52  STGENYPVPLSPPLAAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIY 111
           +TGE YPVP+SPP  A SK++EL RAM AS++S+  A S+  V++EDEWL  V+KP G+Y
Sbjct: 47  ATGE-YPVPVSPPYPAASKDVELRRAMTASARSA--AYSSAPVVFEDEWLAVVDKPAGVY 106

Query: 112 CESVLAAVPRLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWACSELAMLLIVRHV 171
           C+++L+A+P                                      CS   +       
Sbjct: 107 CDALLSALP--------------------------------------CSAATL------- 166

Query: 172 LFSDSLYAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFC 231
                   G + + P LHLANRLDRDTSG+MVITK +KVA KLVKAFT+H V K+Y+A C
Sbjct: 167 --------GDEATKPNLHLANRLDRDTSGLMVITKCNKVAGKLVKAFTEHKVKKTYLALC 226

Query: 232 VGSTPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNVM 291
           +G  P W+KI + SGHGRSK G WRVYA +DVGRSLPGGSVVRDM T FEVL +NGK   
Sbjct: 227 IGYPPAWEKIKICSGHGRSKHGAWRVYAMSDVGRSLPGGSVVRDMSTRFEVLGINGKGQF 286

Query: 292 EGFQKFRKHEEETIVVQTKSLVEIDGQKDE----ILIRARPCSGRTHQIRLHCQYLGIPI 351
                F   E E+I VQ K+  ++    DE    IL+RA P SGRTHQIRLHCQYLG PI
Sbjct: 287 REPSNFEVDETESITVQEKA-ADLTSDGDEKNSIILVRAYPQSGRTHQIRLHCQYLGFPI 339

Query: 352 RGDVKYEGVTEWNGKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQ 398
           RGDVKY GV EWNG  YD H LHAESL FVHPVTG+P+  ++PLPSWA++
Sbjct: 347 RGDVKYSGVIEWNGVDYDGHALHAESLSFVHPVTGLPVTFRSPLPSWANE 339

[Close]

BLAST of CmUC02G040630.1 vs. ExPASy Swiss-Prot
Match: Q7XA65 (RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 4.3e-90
Identity = 175/343 (51.02%), Postives = 219/343 (63.85%), Query Frame = 0


Query: 56  NYPVPLSPPLAAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESV 115
           NYP P+S P   ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 116 LAAVPRLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWACSELAMLLIVRHVLFSD 175
           L + P+++ DS+                                                
Sbjct: 99  LRSAPQIVLDSS------------------------------------------------ 158

Query: 176 SLYAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGST 235
                      E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + KSYIA C+GS+
Sbjct: 159 ----------SEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRKSYIALCIGSS 218

Query: 236 PKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNVMEGFQ 295
           P W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+SVN    ++   
Sbjct: 219 PNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNS---VKNES 278

Query: 296 KFRKHEEETIVVQTKSLVEIDGQKDE--ILIRARPCSGRTHQIRLHCQYLGIPIRGDVKY 355
              +     IV + +  +   G  D+  +++RA P SGRTHQIRLHCQYLGIPIRGDVKY
Sbjct: 279 CELEDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQYLGIPIRGDVKY 320

Query: 356 EGVTEWNGKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWAS 397
            GV EWNG+ ++ HELHAE L   HPVTG  + ++APLP WA+
Sbjct: 339 HGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]

BLAST of CmUC02G040630.1 vs. ExPASy Swiss-Prot
Match: Q8PHN2 (Ribosomal large subunit pseudouridine synthase D OS=Xanthomonas axonopodis pv. citri (strain 306) OX=190486 GN=rluD PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 2.9e-14
Identity = 69/238 (28.99%), Postives = 104/238 (43.70%), Query Frame = 0


Query: 167 IVRHVLFSDSLYAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKS 226
           +V  +LF D   A    S+P   + +RLD+DTSGVMV+ ++ +  + LV+  +   V + 
Sbjct: 121 LVNALLFRDPALA----SVPRAGVVHRLDKDTSGVMVVARTVQAQTALVEQLSAREVHRQ 180

Query: 227 YIAFCVGSTPKWKKINVK-SGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV 286
           Y+A  VG+       N     H R +  +    A  D G         RD  T++ +   
Sbjct: 181 YLAVVVGALVSGGTANAPIDRHPRDRLKM----AVRDDG---------RDAVTHYRLR-- 240

Query: 287 NGKNVMEGFQKFRKHEEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIRLHCQYLGI 346
                    ++FR H                       +  R  +GRTHQIR+H  +L  
Sbjct: 241 ---------ERFRAH---------------------TALECRLETGRTHQIRVHMAHLKS 300

Query: 347 PIRGDVKYEGVTEWNGKVYDS--HE--------LHAESLYFVHPVTGIPLKLQAPLPS 394
           PI GD  Y G  +      D   HE        LHAE+L F+HPV+G P++  AP+P+
Sbjct: 301 PIVGDPLYGGALKLPKGATDELVHELRTFKRQALHAETLEFLHPVSGEPVRASAPVPA 309

[Close]

BLAST of CmUC02G040630.1 vs. ExPASy Swiss-Prot
Match: Q8FIP7 (Ribosomal large subunit pseudouridine synthase C OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=rluC PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.9e-13
Identity = 62/230 (26.96%), Postives = 101/230 (43.91%), Query Frame = 0


Query: 180 GIKTSLPE---LHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSTP 239
           G++   PE   L L +RLDRDTSGV+++ K       L +   +  + K Y+A   G   
Sbjct: 126 GLRALRPEARFLELVHRLDRDTSGVLLVAKKRSALRSLHEQLREKGMQKDYLALVRGQ-- 185

Query: 240 KWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNVMEGFQK 299
                             W+ Y  +     L                    KN+++  ++
Sbjct: 186 ------------------WQSYVKSVQAPLL--------------------KNILQSGER 245

Query: 300 FRKHEEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIRLHCQYLGIPIRGDVKYEGV 359
             +  +E    +T+  VE +      L+R  P +GRTHQIR+H QY G PI  D +Y G 
Sbjct: 246 IVRVSQEGKPSETRFKVE-ERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRY-GD 305

Query: 360 TEWNGKVYDSHE------LHAESLYFVHPVTGIPLKLQAPLPSWASQALQ 401
            E++ ++ ++        LHA +L F HP TG  ++++AP+     + LQ
Sbjct: 306 REFDRQLTEAGTGLNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQ 313

[Close]

BLAST of CmUC02G040630.1 vs. ExPASy Swiss-Prot
Match: Q68XB2 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=rluC PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 2.5e-13
Identity = 64/215 (29.77%), Postives = 99/215 (46.05%), Query Frame = 0


Query: 187 ELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSTPKWKKINVKSG 246
           +  L +RLD++TSG+++I K++  + KL  AF +  V K Y A   G   K   I VKS 
Sbjct: 132 DFKLVHRLDKETSGLLLIAKNYLSSVKLHDAFKEKLVIKKYFAITYGRPVKNVGI-VKSN 191

Query: 247 HGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNVMEGFQKFRKHEEETIV 306
            G+SK    R++   D+      G +     TY+++L     N+                
Sbjct: 192 IGKSK---GRMFKITDIDSD--NGKLA---ITYYKLLKSLNNNL---------------- 251

Query: 307 VQTKSLVEIDGQKDEILIRARPCSGRTHQIRLHCQYLGIPIRGDVKY--EGVTEWNGKVY 366
                           LI   P +GR HQ+RLH Q LG PI GD KY  + V  ++  ++
Sbjct: 252 ---------------FLIEFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEVMPYSKYMF 302

Query: 367 DSHELHAESLYFVHPVTGIPLKLQAPLPSWASQAL 400
               LHA ++Y    + G  +KL+A LP + ++ L
Sbjct: 312 ----LHANNIYLSESIVGKEIKLEAKLPFYFTRRL 302

[Close]

BLAST of CmUC02G040630.1 vs. ExPASy TrEMBL
Match: A0A0A0LJY6 (PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G033960 PE=4 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 3.1e-176
Identity = 328/416 (78.85%), Postives = 338/416 (81.25%), Query Frame = 0


Query: 1   MALFTALQPTFSISHKTLTKFPIPKPFCFHRFKSVSNSMADSSEKVARNCPSTGENYPVP 60
           MALF +L P FSIS KT T+FP  K FCFHRFKSVSNSM DSSE V  NCPST ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLAAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120
           LSPPL AISKNLELARAM ASSKSSL+ALSANDVIYEDEWLIAVNKPQGIYCE+VLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWACSELAMLLIVRHVLFSDSLYAG 180
           RLLGDSA                                                   AG
Sbjct: 121 RLLGDSA--------------------------------------------------NAG 180

Query: 181 IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSTPKWKK 240
           IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVG++PKWKK
Sbjct: 181 IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKK 240

Query: 241 INVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNVMEGFQKFRKH 300
           INVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKN ME  QKFR+ 
Sbjct: 241 INVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNTMEELQKFRRG 300

Query: 301 EEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWN 360
           EEETIVV TKSLV+ID  KDEILIRARP SGRTHQIRLHCQYLGIPIRGDVKYEGVTEWN
Sbjct: 301 EEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWN 360

Query: 361 GKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQ-EVSSPTSFKPMP 416
            K+YDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQ EV+SP SFK MP
Sbjct: 361 EKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHEVNSPQSFKTMP 366

[Close]

BLAST of CmUC02G040630.1 vs. ExPASy TrEMBL
Match: A0A5A7TX25 (RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001190 PE=4 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 1.1e-160
Identity = 300/378 (79.37%), Postives = 310/378 (82.01%), Query Frame = 0


Query: 39  MADSSEKVARNCPSTGENYPVPLSPPLAAISKNLELARAMAASSKSSLFALSANDVIYED 98
           M DSSE V  NCPSTGENYPVPLSPPL AISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWA 158
           EWLIAVNKPQGIYCE+VLAAVPRLL DSA                               
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSA------------------------------- 120

Query: 159 CSELAMLLIVRHVLFSDSLYAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAF 218
                               AGIKT+LPELHLANRLDRDTSGVMVITKSHKVASKLVKAF
Sbjct: 121 -------------------NAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAF 180

Query: 219 TDHTVSKSYIAFCVGSTPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET 278
           TDHTVSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMET
Sbjct: 181 TDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMET 240

Query: 279 YFEVLSVNGKNVMEGFQKFRKHEEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIRL 338
           YFEVLSVNGKN ME  QKFRK EEETIVV TKSLV+ID QKDE+LIRARP SGRTHQIRL
Sbjct: 241 YFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRL 300

Query: 339 HCQYLGIPIRGDVKYEGVTEWNGKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQA 398
           HCQYLGIPIRGDVKYEGVTEWNGK YDSHELHAESLYFVHP+TGIPLKLQAPLPSWASQA
Sbjct: 301 HCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQA 328

Query: 399 LQP-QQEVSSPTSFKPMP 416
           LQP QQEV+SP SFK MP
Sbjct: 361 LQPQQQEVNSPQSFKTMP 328

[Close]

BLAST of CmUC02G040630.1 vs. ExPASy TrEMBL
Match: A0A1S3BW87 (RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 1.1e-160
Identity = 300/378 (79.37%), Postives = 310/378 (82.01%), Query Frame = 0


Query: 39  MADSSEKVARNCPSTGENYPVPLSPPLAAISKNLELARAMAASSKSSLFALSANDVIYED 98
           M DSSE V  NCPSTGENYPVPLSPPL AISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWA 158
           EWLIAVNKPQGIYCE+VLAAVPRLL DSA                               
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSA------------------------------- 120

Query: 159 CSELAMLLIVRHVLFSDSLYAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAF 218
                               AGIKT+LPELHLANRLDRDTSGVMVITKSHKVASKLVKAF
Sbjct: 121 -------------------NAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAF 180

Query: 219 TDHTVSKSYIAFCVGSTPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET 278
           TDHTVSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMET
Sbjct: 181 TDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMET 240

Query: 279 YFEVLSVNGKNVMEGFQKFRKHEEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIRL 338
           YFEVLSVNGKN ME  QKFRK EEETIVV TKSLV+ID QKDE+LIRARP SGRTHQIRL
Sbjct: 241 YFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRL 300

Query: 339 HCQYLGIPIRGDVKYEGVTEWNGKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQA 398
           HCQYLGIPIRGDVKYEGVTEWNGK YDSHELHAESLYFVHP+TGIPLKLQAPLPSWASQA
Sbjct: 301 HCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQA 328

Query: 399 LQP-QQEVSSPTSFKPMP 416
           LQP QQEV+SP SFK MP
Sbjct: 361 LQPQQQEVNSPQSFKTMP 328

[Close]

BLAST of CmUC02G040630.1 vs. ExPASy TrEMBL
Match: A0A6J1DT56 (RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 2.0e-151
Identity = 287/375 (76.53%), Postives = 307/375 (81.87%), Query Frame = 0


Query: 39  MADSSEKVARNCPSTGENYPVPLSPPLAAISKNLELARAMAASSKSSLFALSANDVIYED 98
           MAD S  V  N P+TG+NYP+PLSPPL AISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 99  EWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWA 158
           EWLIAVNKPQG+YCESVLA+VPRLLGDSAMAG                  E SG      
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG------------------EQSG-----G 120

Query: 159 CSELAMLLIVRHVLFSDSLYAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAF 218
            + L + L            A I+ SLPELHLANRLDRDTSGVMVITKSHKVASKLVKAF
Sbjct: 121 TTTLCLCL------------ARIQISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAF 180

Query: 219 TDHTVSKSYIAFCVGSTPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET 278
           TDH VSKSYIAFCVGS PKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET
Sbjct: 181 TDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET 240

Query: 279 YFEVLSVNGKNVMEGFQKFRKHEEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIRL 338
           YFEVLSVNG++ M+   + RK EEETIVVQ+KSL++ID  KDEILIRARP SGRTHQIRL
Sbjct: 241 YFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEILIRARPRSGRTHQIRL 300

Query: 339 HCQYLGIPIRGDVKYEGVTEWNGKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQA 398
           HCQYLGIPIRGDVKYEGV EWNG +YDSHELHAESL+FVHPVTGIPLKLQAPLPSWASQA
Sbjct: 301 HCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQAPLPSWASQA 340

Query: 399 -LQPQQEVSSPTSFK 413
            L  QQEVSSP++ K
Sbjct: 361 LLSQQQEVSSPSTLK 340

[Close]

BLAST of CmUC02G040630.1 vs. ExPASy TrEMBL
Match: A0A6J1DQ12 (RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 4.5e-151
Identity = 285/375 (76.00%), Postives = 303/375 (80.80%), Query Frame = 0


Query: 39  MADSSEKVARNCPSTGENYPVPLSPPLAAISKNLELARAMAASSKSSLFALSANDVIYED 98
           MAD S  V  N P+TG+NYP+PLSPPL AISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 99  EWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWA 158
           EWLIAVNKPQG+YCESVLA+VPRLLGDSAMAG                  E SG      
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG------------------EQSG------ 120

Query: 159 CSELAMLLIVRHVLFSDSLYAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAF 218
                                GI+ SLPELHLANRLDRDTSGVMVITKSHKVASKLVKAF
Sbjct: 121 ---------------------GIQISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAF 180

Query: 219 TDHTVSKSYIAFCVGSTPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET 278
           TDH VSKSYIAFCVGS PKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET
Sbjct: 181 TDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET 240

Query: 279 YFEVLSVNGKNVMEGFQKFRKHEEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIRL 338
           YFEVLSVNG++ M+   + RK EEETIVVQ+KSL++ID  KDEILIRARP SGRTHQIRL
Sbjct: 241 YFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEILIRARPRSGRTHQIRL 300

Query: 339 HCQYLGIPIRGDVKYEGVTEWNGKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQA 398
           HCQYLGIPIRGDVKYEGV EWNG +YDSHELHAESL+FVHPVTGIPLKLQAPLPSWASQA
Sbjct: 301 HCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQAPLPSWASQA 330

Query: 399 -LQPQQEVSSPTSFK 413
            L  QQEVSSP++ K
Sbjct: 361 LLSQQQEVSSPSTLK 330

[Close]

BLAST of CmUC02G040630.1 vs. TAIR 10
Match: AT1G56345.1 (Pseudouridine synthase family protein )

HSP 1 Score: 333.2 bits (853), Expect = 3.0e-91
Identity = 175/343 (51.02%), Postives = 219/343 (63.85%), Query Frame = 0


Query: 56  NYPVPLSPPLAAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESV 115
           NYP P+S P   ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 116 LAAVPRLLGDSAMAGLWILLYNRVFSMDYKISSEISGYHDRWACSELAMLLIVRHVLFSD 175
           L + P+++ DS+                                                
Sbjct: 99  LRSAPQIVLDSS------------------------------------------------ 158

Query: 176 SLYAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGST 235
                      E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + KSYIA C+GS+
Sbjct: 159 ----------SEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRKSYIALCIGSS 218

Query: 236 PKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNVMEGFQ 295
           P W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+SVN    ++   
Sbjct: 219 PNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNS---VKNES 278

Query: 296 KFRKHEEETIVVQTKSLVEIDGQKDE--ILIRARPCSGRTHQIRLHCQYLGIPIRGDVKY 355
              +     IV + +  +   G  D+  +++RA P SGRTHQIRLHCQYLGIPIRGDVKY
Sbjct: 279 CELEDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQYLGIPIRGDVKY 320

Query: 356 EGVTEWNGKVYDSHELHAESLYFVHPVTGIPLKLQAPLPSWAS 397
            GV EWNG+ ++ HELHAE L   HPVTG  + ++APLP WA+
Sbjct: 339 HGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]

BLAST of CmUC02G040630.1 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 75.5 bits (184), Expect = 1.1e-13
Identity = 80/321 (24.92%), Postives = 125/321 (38.94%), Query Frame = 0


Query: 93  DVIYEDEWLIAVNKPQGIYCESVLAAVPRLLGDSAMAGLWILLYNRVFSMDYKISSEISG 152
           D++YED+ ++ VNKP       V+   P     + + G  IL +  +  +DY  S E   
Sbjct: 149 DIVYEDKHVLVVNKP----AHMVVHPAPGNPTGTLVNG--ILHHCSLPCVDYSNSEEDDD 208

Query: 153 YHDRWACSELAMLLIVRHVLFSDSLYAGIKTSLPELHLANRLDRDTSGVMVITKSHKVAS 212
             +     +  M         S S YA    +     + +RLD+ T+G++V+ K     +
Sbjct: 209 SDEETFSDDEEMTT-------SPSSYA----ASVRPGIVHRLDKGTTGLLVVAKDEHSHA 268

Query: 213 KLVKAFTDHTVSKSYIAFCVG-STPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGS 272
            L + F  HT+ + Y++   G  +P   +I +  G   S     R+  AA     +PGG 
Sbjct: 269 HLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSN----RIRMAA-----IPGG- 328

Query: 273 VVRDMETYFEVLSVNGKNVMEGFQKFRKHEEETIVVQTKSLVEIDGQKDEILIRARPCSG 332
                        V G        +++             ++E        L+  R  +G
Sbjct: 329 -------------VRGGRARHAASRYK-------------VIETFAGGGSALVEWRLETG 388

Query: 333 RTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKVY-------DSHE------------LHAE 392
           RTHQIR H +Y+G+P+ GD  Y G       +        D  E            LHA 
Sbjct: 389 RTHQIRAHAKYMGVPLLGDEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAI 416

Query: 393 SLYFVHPVTGIPLKLQAPLPS 394
            L F HP TG  +K   P PS
Sbjct: 449 VLGFTHPCTGEIVKFSCPPPS 416

[Close]

BLAST of CmUC02G040630.1 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 1.2e-07
Identity = 58/197 (29.44%), Postives = 82/197 (41.62%), Query Frame = 0


Query: 179 AGIKTSLPEL-------------HLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 238
           +GIKTS+ EL              L +RLDRD SG++V+ ++   A+ L   F + T   
Sbjct: 207 SGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVLHSIFREKT--- 266

Query: 239 SYIAFCVGSTPKWKKINVKSGHGRSKFGVWRVYAAADVG--------RSLPGGSVVRDME 298
                  G++    K NVKS        + R Y A  +G         S P   VV D +
Sbjct: 267 ------TGASAYGVKKNVKS--------LKRKYMALVIGCPPRQRGQISAPLRKVVVD-D 326

Query: 299 TYFEVLSVNGKNVMEGFQKFRKHEEETIVVQTKSLVEIDGQKDEILIRARPCSGRTHQIR 354
              E ++VN    +   Q             T+  V          +  RP +GR HQ+R
Sbjct: 327 GKSERITVNDNGELVSTQH----------AITEYRVIESSPHGYTWLELRPLTGRKHQLR 375

[Close]

BLAST of CmUC02G040630.1 vs. TAIR 10
Match: AT5G51140.1 (Pseudouridine synthase family protein )

HSP 1 Score: 52.0 bits (123), Expect = 1.3e-06
Identity = 50/188 (26.60%), Postives = 70/188 (37.23%), Query Frame = 0


Query: 192 NRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSTPKWKKINVKSGHGRSK 251
           +RLDR  SG+++I ++   A    +      V K YIA  +G  P+ + I          
Sbjct: 184 HRLDRLVSGLLIIARTAAKADFFRQQIEGGMVKKRYIAKVIGVFPEDEMI---------- 243

Query: 252 FGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNVMEGFQKFRKHEEETIVVQTKS 311
                V A  +   S  G S   D  +  +   V GK     F +               
Sbjct: 244 -----VDANINYNGS-EGRSTAEDANSSGDDKKVKGKPACTKFTR--------------- 303

Query: 312 LVEIDGQKDEILIRARPCSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKVYDSHELHA 371
              ID      L+   P +GRTHQIR+H QY G PI  D  Y      N + Y +  + A
Sbjct: 304 ---IDTNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLYLNQHIDNLETYIAKRIDA 337

Query: 372 ESLYFVHP 380
                V P
Sbjct: 364 GERKIVSP 337

[Close]

BLAST of CmUC02G040630.1 vs. TAIR 10
Match: AT5G51140.2 (Pseudouridine synthase family protein )

HSP 1 Score: 52.0 bits (123), Expect = 1.3e-06
Identity = 50/188 (26.60%), Postives = 70/188 (37.23%), Query Frame = 0


Query: 192 NRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSTPKWKKINVKSGHGRSK 251
           +RLDR  SG+++I ++   A    +      V K YIA  +G  P+ + I          
Sbjct: 184 HRLDRLVSGLLIIARTAAKADFFRQQIEGGMVKKRYIAKVIGVFPEDEMI---------- 243

Query: 252 FGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNVMEGFQKFRKHEEETIVVQTKS 311
                V A  +   S  G S   D  +  +   V GK     F +               
Sbjct: 244 -----VDANINYNGS-EGRSTAEDANSSGDDKKVKGKPACTKFTR--------------- 303

Query: 312 LVEIDGQKDEILIRARPCSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKVYDSHELHA 371
              ID      L+   P +GRTHQIR+H QY G PI  D  Y      N + Y +  + A
Sbjct: 304 ---IDTNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLYLNQHIDNLETYIAKRIDA 337

Query: 372 ESLYFVHP 380
                V P
Sbjct: 364 GERKIVSP 337

The following BLAST results are available for this feature:

BLAST of CmUC02G040630.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144814.3	6.4e-176	78.85	RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein ...	[more]
XP_038889468.1	2.8e-171	78.73	RNA pseudouridine synthase 1 [Benincasa hispida]	[more]
XP_008453651.1	2.2e-160	79.37	PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudou...	[more]
KAG7021056.1	3.0e-157	72.86	RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]
KAG6586224.1	3.6e-155	72.51	RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmUC02G040630.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2QNM3	1.2e-92	52.29	RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g05...	[more]
Q7XA65	4.3e-90	51.02	RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 S...	[more]
Q8PHN2	2.9e-14	28.99	Ribosomal large subunit pseudouridine synthase D OS=Xanthomonas axonopodis pv. c...	[more]
Q8FIP7	1.9e-13	26.96	Ribosomal large subunit pseudouridine synthase C OS=Escherichia coli O6:H1 (stra...	[more]
Q68XB2	2.5e-13	29.77	Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATC...	[more]

BLAST of CmUC02G040630.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJY6	3.1e-176	78.85	PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G03...	[more]
A0A5A7TX25	1.1e-160	79.37	RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BW87	1.1e-160	79.37	RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1	[more]
A0A6J1DT56	2.0e-151	76.53	RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1DQ12	4.5e-151	76.00	RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of CmUC02G040630.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56345.1	3.0e-91	51.02	Pseudouridine synthase family protein	[more]
AT1G76050.2	1.1e-13	24.92	Pseudouridine synthase family protein	[more]
AT3G19440.1	1.2e-07	29.44	Pseudouridine synthase family protein	[more]
AT5G51140.1	1.3e-06	26.60	Pseudouridine synthase family protein	[more]
AT5G51140.2	1.3e-06	26.60	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.2350.10	Pseudouridine synthase	coord: 89..392 e-value: 3.7E-42 score: 146.3
None	No IPR available	PANTHER	PTHR21600	MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE	coord: 39..402
None	No IPR available	PANTHER	PTHR21600:SF47	RNA PSEUDOURIDINE SYNTHASE 1	coord: 39..402
IPR006145	Pseudouridine synthase, RsuA/RluA-like	PFAM	PF00849	PseudoU_synth_2	coord: 104..341 e-value: 5.5E-21 score: 75.3
IPR006145	Pseudouridine synthase, RsuA/RluA-like	CDD	cd02869	PseudoU_synth_RluA_like	coord: 186..376 e-value: 2.17729E-34 score: 124.372
IPR006224	Pseudouridine synthase, RluC/RluD, conserved site	PROSITE	PS01129	PSI_RLU	coord: 191..205
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 92..400

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmUC02G040630	CmUC02G040630	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmUC02G040630.1-exon	CmUC02G040630.1-exon-CmU531Chr02:28419010..28419413	exon
CmUC02G040630.1-exon	CmUC02G040630.1-exon-CmU531Chr02:28419566..28419598	exon
CmUC02G040630.1-exon	CmUC02G040630.1-exon-CmU531Chr02:28419683..28419687	exon
CmUC02G040630.1-exon	CmUC02G040630.1-exon-CmU531Chr02:28419753..28419765	exon
CmUC02G040630.1-exon	CmUC02G040630.1-exon-CmU531Chr02:28420417..28420442	exon
CmUC02G040630.1-exon	CmUC02G040630.1-exon-CmU531Chr02:28420518..28420545	exon
CmUC02G040630.1-exon	CmUC02G040630.1-exon-CmU531Chr02:28420687..28421425	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmUC02G040630.1-cds	CmUC02G040630.1-cds-CmU531Chr02:28419010..28419413	CDS
CmUC02G040630.1-cds	CmUC02G040630.1-cds-CmU531Chr02:28419566..28419598	CDS
CmUC02G040630.1-cds	CmUC02G040630.1-cds-CmU531Chr02:28419683..28419687	CDS
CmUC02G040630.1-cds	CmUC02G040630.1-cds-CmU531Chr02:28419753..28419765	CDS
CmUC02G040630.1-cds	CmUC02G040630.1-cds-CmU531Chr02:28420417..28420442	CDS
CmUC02G040630.1-cds	CmUC02G040630.1-cds-CmU531Chr02:28420518..28420545	CDS
CmUC02G040630.1-cds	CmUC02G040630.1-cds-CmU531Chr02:28420687..28421425	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmUC02G040630.1	CmUC02G040630.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding