Carg19284-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19284-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	E3 ubiquitin-protein ligase arkadia-A, putative
Location	Carg_Chr03: 9777763 .. 9778131 (+)
Sequence length	369
RNA-Seq Expression	Carg19284-RA
Synteny	Carg19284-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACGACCCTCATAAGCTTCAGAACCTACCCTCTTTCAAACTCTCCCGCTACTCCTCTACCGTCCGAGATTCCGACGCCGGCGATGACCTCCGCTACACCAGCTTGAAGGACATTCTTCTGAACTCCCCGTCCTACAGCGGCGTTAATGAGTTCAACGAGTTCAATTTGTCGAACATTACGATCCGAAACGAGCTCGTGAAAAGGGCCGCGTCGGCGTATCTTCAATCGGCGGCGATCTTGGTCGGCCGGAACGAGAGCTGTTTCGTGGGATTCTGGGAGAGGCTTAGGATCAAACTCGCCGCCCTCCGCTCCCGTTGGTGTGATTGCTTTCCCCGCTTTTTTGGCTTCTTTAGGTTTGTCTTTTAG

mRNA sequence

ATGGACGACCCTCATAAGCTTCAGAACCTACCCTCTTTCAAACTCTCCCGCTACTCCTCTACCGTCCGAGATTCCGACGCCGGCGATGACCTCCGCTACACCAGCTTGAAGGACATTCTTCTGAACTCCCCGTCCTACAGCGGCGTTAATGAGTTCAACGAGTTCAATTTGTCGAACATTACGATCCGAAACGAGCTCGTGAAAAGGGCCGCGTCGGCGTATCTTCAATCGGCGGCGATCTTGGTCGGCCGGAACGAGAGCTGTTTCGTGGGATTCTGGGAGAGGCTTAGGATCAAACTCGCCGCCCTCCGCTCCCGTTGGTGTGATTGCTTTCCCCGCTTTTTTGGCTTCTTTAGGTTTGTCTTTTAG

Coding sequence (CDS)

ATGGACGACCCTCATAAGCTTCAGAACCTACCCTCTTTCAAACTCTCCCGCTACTCCTCTACCGTCCGAGATTCCGACGCCGGCGATGACCTCCGCTACACCAGCTTGAAGGACATTCTTCTGAACTCCCCGTCCTACAGCGGCGTTAATGAGTTCAACGAGTTCAATTTGTCGAACATTACGATCCGAAACGAGCTCGTGAAAAGGGCCGCGTCGGCGTATCTTCAATCGGCGGCGATCTTGGTCGGCCGGAACGAGAGCTGTTTCGTGGGATTCTGGGAGAGGCTTAGGATCAAACTCGCCGCCCTCCGCTCCCGTTGGTGTGATTGCTTTCCCCGCTTTTTTGGCTTCTTTAGGTTTGTCTTTTAG

Protein sequence

MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNITIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRFVF

Homology

[Close]

BLAST of Carg19284-RA vs. NCBI nr
Match: XP_022925985.1 (uncharacterized protein LOC111433238 [Cucurbita moschata] >KAG6581442.1 hypothetical protein SDJN03_21444, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 249.2 bits (635), Expect = 1.8e-62
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 60
           MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI
Sbjct: 10  MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 69

Query: 61  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 120
           TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF
Sbjct: 70  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 129

Query: 121 VF 123
           VF
Sbjct: 130 VF 131

[Close]

BLAST of Carg19284-RA vs. NCBI nr
Match: KAG7034735.1 (hypothetical protein SDJN02_04465, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 249.2 bits (635), Expect = 1.8e-62
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 60
           MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI
Sbjct: 1   MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 60

Query: 61  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 120
           TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF
Sbjct: 61  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 120

Query: 121 VF 123
           VF
Sbjct: 121 VF 122

[Close]

BLAST of Carg19284-RA vs. NCBI nr
Match: XP_022977989.1 (uncharacterized protein LOC111478115 [Cucurbita maxima])

HSP 1 Score: 247.7 bits (631), Expect = 5.3e-62
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = 0


Query: 1   MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 60
           MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI
Sbjct: 10  MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 69

Query: 61  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 120
           TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF
Sbjct: 70  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 129

Query: 121 VF 123
            F
Sbjct: 130 AF 131

[Close]

BLAST of Carg19284-RA vs. NCBI nr
Match: XP_023544918.1 (uncharacterized protein LOC111804374 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 247.7 bits (631), Expect = 5.3e-62
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = 0


Query: 1   MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 60
           MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI
Sbjct: 65  MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 124

Query: 61  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 120
           TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF
Sbjct: 125 TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 184

Query: 121 VF 123
            F
Sbjct: 185 AF 186

[Close]

BLAST of Carg19284-RA vs. NCBI nr
Match: KAA0052530.1 (putative E3 ubiquitin-protein ligase arkadia-A [Cucumis melo var. makuwa])

HSP 1 Score: 217.2 bits (552), Expect = 7.6e-53
Identity = 107/120 (89.17%), Postives = 112/120 (93.33%), Query Frame = 0


Query: 1   MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 60
           M+D HKLQN+PSFKLSRYSS+ RDSDAGDDLRYTSLKDILLNSPSYSGVN+FNEFN SNI
Sbjct: 7   MEDSHKLQNIPSFKLSRYSSSARDSDAGDDLRYTSLKDILLNSPSYSGVNDFNEFNSSNI 66

Query: 61  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 120
           +IRNELVKRAASAYLQSAAILV RNES FVGFWERLRIKLAALRSRWCDCF R FGFF F
Sbjct: 67  SIRNELVKRAASAYLQSAAILVSRNESGFVGFWERLRIKLAALRSRWCDCFHRIFGFFSF 126

[Close]

BLAST of Carg19284-RA vs. ExPASy TrEMBL
Match: A0A6J1EJR7 (uncharacterized protein LOC111433238 OS=Cucurbita moschata OX=3662 GN=LOC111433238 PE=4 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 8.8e-63
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 60
           MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI
Sbjct: 10  MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 69

Query: 61  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 120
           TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF
Sbjct: 70  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 129

Query: 121 VF 123
           VF
Sbjct: 130 VF 131

[Close]

BLAST of Carg19284-RA vs. ExPASy TrEMBL
Match: A0A6J1ISV4 (uncharacterized protein LOC111478115 OS=Cucurbita maxima OX=3661 GN=LOC111478115 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.6e-62
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = 0


Query: 1   MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 60
           MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI
Sbjct: 10  MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 69

Query: 61  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 120
           TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF
Sbjct: 70  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 129

Query: 121 VF 123
            F
Sbjct: 130 AF 131

[Close]

BLAST of Carg19284-RA vs. ExPASy TrEMBL
Match: A0A5A7UBK0 (Putative E3 ubiquitin-protein ligase arkadia-A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G001310 PE=4 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.7e-53
Identity = 107/120 (89.17%), Postives = 112/120 (93.33%), Query Frame = 0


Query: 1   MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 60
           M+D HKLQN+PSFKLSRYSS+ RDSDAGDDLRYTSLKDILLNSPSYSGVN+FNEFN SNI
Sbjct: 7   MEDSHKLQNIPSFKLSRYSSSARDSDAGDDLRYTSLKDILLNSPSYSGVNDFNEFNSSNI 66

Query: 61  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 120
           +IRNELVKRAASAYLQSAAILV RNES FVGFWERLRIKLAALRSRWCDCF R FGFF F
Sbjct: 67  SIRNELVKRAASAYLQSAAILVSRNESGFVGFWERLRIKLAALRSRWCDCFHRIFGFFSF 126

[Close]

BLAST of Carg19284-RA vs. ExPASy TrEMBL
Match: A0A0A0KKP9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G524030 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 4.8e-53
Identity = 106/120 (88.33%), Postives = 112/120 (93.33%), Query Frame = 0


Query: 1   MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 60
           M+DPH+LQN+PSFKLSRYSS+ RDSDA DDLRYTSLKDILLNSPSYSGVN+FNEFN SNI
Sbjct: 9   MEDPHRLQNIPSFKLSRYSSSARDSDASDDLRYTSLKDILLNSPSYSGVNDFNEFNSSNI 68

Query: 61  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSRWCDCFPRFFGFFRF 120
           +IRNELVKRAASAYLQSAAILV RNES FVGFWERLRIKLAALRSRWCDCF R FGFF F
Sbjct: 69  SIRNELVKRAASAYLQSAAILVSRNESGFVGFWERLRIKLAALRSRWCDCFHRIFGFFSF 128

[Close]

BLAST of Carg19284-RA vs. ExPASy TrEMBL
Match: A0A6J1CYB8 (uncharacterized protein LOC111015712 OS=Momordica charantia OX=3673 GN=LOC111015712 PE=4 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.0e-42
Identity = 91/106 (85.85%), Postives = 97/106 (91.51%), Query Frame = 0


Query: 1   MDDPHKLQNLPSFKLSRYSSTVRDSDAGDDLRYTSLKDILLNSPSYSGVNEFNEFNLSNI 60
           + + HKLQN+PSFKLSRYSS  RDSDAGDDLRY SLKDILLNSPSYSGVN+FN+FN SNI
Sbjct: 4   ISESHKLQNIPSFKLSRYSSGARDSDAGDDLRYISLKDILLNSPSYSGVNDFNDFNSSNI 63

Query: 61  TIRNELVKRAASAYLQSAAILVGRNESCFVGFWERLRIKLAALRSR 107
           +IRNELVKRAASAYLQSAAILV RNESCFV FWERLR KLAALRSR
Sbjct: 64  SIRNELVKRAASAYLQSAAILVSRNESCFVSFWERLRNKLAALRSR 109

[Close]

BLAST of Carg19284-RA vs. TAIR 10
Match: AT4G18395.1 (unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 52.4 bits (124), Expect = 3.0e-07
Identity = 32/84 (38.10%), Postives = 50/84 (59.52%), Query Frame = 0


Query: 22 VRDSDAGDDLRYTSLKDILLNS---------PSYSGVNE----FNEFNLSNITIRNELVK 81
          + D +  ++L+YTSLKD++ +S         P YS  ++     +E + SNI I NELVK
Sbjct: 10 ISDDNDQNNLQYTSLKDVISSSDGFGSLFCHPQYSVPSQDGFLLSEMDSSNIAISNELVK 69

Query: 82 RAASAYLQSAAILVGRNESCFVGF 93
          RAAS Y+QS+ I++  + + F  F
Sbjct: 70 RAASMYVQSSMIVIAPDTNWFQRF 93

The following BLAST results are available for this feature:

BLAST of Carg19284-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022925985.1	1.8e-62	100.00	uncharacterized protein LOC111433238 [Cucurbita moschata] >KAG6581442.1 hypothet...	[more]
KAG7034735.1	1.8e-62	100.00	hypothetical protein SDJN02_04465, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022977989.1	5.3e-62	99.18	uncharacterized protein LOC111478115 [Cucurbita maxima]	[more]
XP_023544918.1	5.3e-62	99.18	uncharacterized protein LOC111804374 [Cucurbita pepo subsp. pepo]	[more]
KAA0052530.1	7.6e-53	89.17	putative E3 ubiquitin-protein ligase arkadia-A [Cucumis melo var. makuwa]	[more]

BLAST of Carg19284-RA vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg19284-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EJR7	8.8e-63	100.00	uncharacterized protein LOC111433238 OS=Cucurbita moschata OX=3662 GN=LOC1114332...	[more]
A0A6J1ISV4	2.6e-62	99.18	uncharacterized protein LOC111478115 OS=Cucurbita maxima OX=3661 GN=LOC111478115...	[more]
A0A5A7UBK0	3.7e-53	89.17	Putative E3 ubiquitin-protein ligase arkadia-A OS=Cucumis melo var. makuwa OX=11...	[more]
A0A0A0KKP9	4.8e-53	88.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G524030 PE=4 SV=1	[more]
A0A6J1CYB8	1.0e-42	85.85	uncharacterized protein LOC111015712 OS=Momordica charantia OX=3673 GN=LOC111015...	[more]

BLAST of Carg19284-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18395.1	3.0e-07	38.10	unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae -...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34569	EXPRESSED PROTEIN	coord: 2..121
None	No IPR available	PANTHER	PTHR34569:SF15	UBIQUITIN-PROTEIN LIGASE ARKADIA-A, PUTATIVE-RELATED	coord: 2..121

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg19284	Carg19284	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg19284-RA:1	Carg19284-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg19284-RA:cds	Carg19284-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg19284-RA	Carg19284-RA-protein	polypeptide