Carg12148-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12148-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	NAD(+) diphosphatase
Location	Carg_Chr11: 2463570 .. 2465907 (+)
Sequence length	1287
RNA-Seq Expression	Carg12148-RA
Synteny	Carg12148-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAACGGCGCAAGAACATTCCTCACTGTATCCAAATCGACTTCTCGACTGCTTCGTCTTCCTCCTGCTTCGTCTCGTTTCTTCACGCCGTTTAACTCCTCCTGCAATGGAGCAACCTTCTGTTCCTCCACCGGAACCCCTTCCATGTCCATTAATTTACAGTCACATGCCTTTGCGCGAAACCCTATAAGATCCAAAACCCCCAATCCTGAAAATCCCCTTTCACCCGATGCGTCGTTCGAATCGCTGAAATCGAAGCTTCTGGCGAAAACCCCTTTGCTATCTTCACCAGATTTCAAAATTTTGCCCTTCAGGAAGGGCTGGCCGTTGGTTTCTTGCAATGGTGGTAAGAGCAACGGTGGTTTGGCGTCTGGGTGGGGCCTGGGTTGGATCAGCTTGGCTGATTGCGAAAGTCTATTGGCCTTGTCTGGGTCGAAGGTAACAGAAGATTTGTTGGTTTATCTCGGTTCTTCTGTTGAAGACGATGCTGTTTATTGGGCCACTGATTTCTCTGGCGCGGATAATTTGGATGCGGAATTGGGTCAAAGGAAGCTCAATTTTATCGAGCTGCGAATGCTCATGGTAGCCACGAATTGGTCGGATACATGGGCAATGGGACAGTTGGCTATTGCTGGTCATGTAAGTGCTCAGAATTGGTTTTTCAATTTGGAGTATGAACATCGTTTCTTAAACTTCTTAATTACTTAAAATGTGGTTGAATCTGTGCGTACCTTTCTGGTTTGTTCGATTGATTGCTCAGGGCAGGGCTTTGCTAGATTGGCATAGAACATCACGTTTCTGTGGGCATTGTGGAGAAAGGACAGTCCCAATGGAAGCTGGGAAGCGAAGGGAATGCTCCAATCAGTCATGCAAGTTGAGGATTTTCCCTCGTGTCGATCCTGTGAGATCATAATCCTTTGCTTTTATGATTGCTGAACCTCTGCATACTGAAGGCTATCTATTGATCTTGTTCATTGCAGGTTGTTATCATGTTAGTCATTGACCAGGAGAACGACCGCGCACTCTTAAGCAGGCAATCGAGATATGTGCCCCGAAAATGGAGTTGCTTAGCTGGTTTTATGGAGGTACCTTTCTTTGCCAATGGCAAGAAGAATGATAGAAATACATTTGATAGAATAAGACTTCTAGCACTAATGAATATGACTAGTTTATTTGTAGTATGATATCATGTGTTGTGAGATCTCACGTCGGTTAGGGAGGAGAACAAAACATCCCTTGGGTGTGGAAACCTATCCCTAGTAGACGCGTTTTAAAACCTTGAGGGAAGCCTAAGAGGGAAAGACCAAATAGGACAATATCTGCTAACGGTGGGCTTGAGCTGTGACAAATGGTATCTGAGCAGCGGTGTGCCAGTGAGGACGCTGGGCTCTCAAGAGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAGGGGAACAAAGCATCCTTATAGGGGTGTGAAAACCCCTCTCTAGTATAGACGAGTTCTAAAACTTTGAGGGAAAGCTCAAAGAGGACGATATTTGCTAGCAGTGGACTTGTTTGTTACGTGTATTTCATGAACGAGAGAATTCATTGATTTTCTTTTGTAATTGTCATCAGCCTGGAGAAAGCTTGGAAGAAGCTGTGATAAGAGAAACATTGGAAGAGACTGGTATTGAAGTTGGAAAGGTGGTCTATCACAGTTCTCAGCCATGGCCAGGTCTGTTCATTCTGAAAGTACAGCTTCTTAACCACGCCTTTGCACTATCTGTTGGTACTTTCGAAACAAAAACGCTCATTTTTCATGTTTCTCATCGAGATATATATATCTGTAATCTCTCTTTTAGGCCATCAGTTTGAATCTTCGTTTAAATTATAATTCTTTCTCAGTTGGACGAAGCACAATGCCGTGTCAATTGATGGTTGGGTTCTTTGCGTATGCAAAATCGCTCGATATCAAGGTGGACAAGGCTGAGTTAGAAGGTATATTGACTAATGTTCACATTATTTGCATTATAATGTCGCCACTTCTAGTTTTCTTTCTAAGACAACACCTCTTGAAATTCTCGTTCACTTTGAATGGATCTGAATTGGTTGAAACTGTGTGTACAGACGCCCAATGGCACACTAGAGAAGATGTGAAGAAAGCTTTAACATTAAGCGACTACGAGAAGACGCAAAGATCTGCAGCAGCCAAAGTCGAACAACTGTGCAACGGAGTTGAAAGCAGACACAGTCAGTCTGCTGATATCAACGCTGAAGATGGTGGACTTGCTTCCATGTTCTTTCCTGGCCCTTATGCAATCGCTCATCAACTTATCTCCTCTTGGGCTTATCAAGACAATGAAAAATGA

mRNA sequence

ATGGCGAACGGCGCAAGAACATTCCTCACTGTATCCAAATCGACTTCTCGACTGCTTCGTCTTCCTCCTGCTTCGTCTCGTTTCTTCACGCCGTTTAACTCCTCCTGCAATGGAGCAACCTTCTGTTCCTCCACCGGAACCCCTTCCATGTCCATTAATTTACAGTCACATGCCTTTGCGCGAAACCCTATAAGATCCAAAACCCCCAATCCTGAAAATCCCCTTTCACCCGATGCGTCGTTCGAATCGCTGAAATCGAAGCTTCTGGCGAAAACCCCTTTGCTATCTTCACCAGATTTCAAAATTTTGCCCTTCAGGAAGGGCTGGCCGTTGGTTTCTTGCAATGGTGGTAAGAGCAACGGTGGTTTGGCGTCTGGGTGGGGCCTGGGTTGGATCAGCTTGGCTGATTGCGAAAGTCTATTGGCCTTGTCTGGGTCGAAGGTAACAGAAGATTTGTTGGTTTATCTCGGTTCTTCTGTTGAAGACGATGCTGTTTATTGGGCCACTGATTTCTCTGGCGCGGATAATTTGGATGCGGAATTGGGTCAAAGGAAGCTCAATTTTATCGAGCTGCGAATGCTCATGGTAGCCACGAATTGGTCGGATACATGGGCAATGGGACAGTTGGCTATTGCTGGTCATGGCAGGGCTTTGCTAGATTGGCATAGAACATCACGTTTCTGTGGGCATTGTGGAGAAAGGACAGTCCCAATGGAAGCTGGGAAGCGAAGGGAATGCTCCAATCAGTCATGCAAGTTGAGGATTTTCCCTCGTGTCGATCCTGTTGTTATCATGTTAGTCATTGACCAGGAGAACGACCGCGCACTCTTAAGCAGGCAATCGAGATATGTGCCCCGAAAATGGAGTTGCTTAGCTGGTTTTATGGAGCCATGGCCAGGTCTGTTCATTCTGAAAGTACAGCTTCTTAACCACGCCTTTGCACTATCTGTTGTTGGACGAAGCACAATGCCGTGTCAATTGATGGTTGGGTTCTTTGCGTATGCAAAATCGCTCGATATCAAGGTGGACAAGGCTGAGTTAGAAGACGCCCAATGGCACACTAGAGAAGATGTGAAGAAAGCTTTAACATTAAGCGACTACGAGAAGACGCAAAGATCTGCAGCAGCCAAAGTCGAACAACTGTGCAACGGAGTTGAAAGCAGACACAGTCAGTCTGCTGATATCAACGCTGAAGATGGTGGACTTGCTTCCATGTTCTTTCCTGGCCCTTATGCAATCGCTCATCAACTTATCTCCTCTTGGGCTTATCAAGACAATGAAAAATGA

Coding sequence (CDS)

ATGGCGAACGGCGCAAGAACATTCCTCACTGTATCCAAATCGACTTCTCGACTGCTTCGTCTTCCTCCTGCTTCGTCTCGTTTCTTCACGCCGTTTAACTCCTCCTGCAATGGAGCAACCTTCTGTTCCTCCACCGGAACCCCTTCCATGTCCATTAATTTACAGTCACATGCCTTTGCGCGAAACCCTATAAGATCCAAAACCCCCAATCCTGAAAATCCCCTTTCACCCGATGCGTCGTTCGAATCGCTGAAATCGAAGCTTCTGGCGAAAACCCCTTTGCTATCTTCACCAGATTTCAAAATTTTGCCCTTCAGGAAGGGCTGGCCGTTGGTTTCTTGCAATGGTGGTAAGAGCAACGGTGGTTTGGCGTCTGGGTGGGGCCTGGGTTGGATCAGCTTGGCTGATTGCGAAAGTCTATTGGCCTTGTCTGGGTCGAAGGTAACAGAAGATTTGTTGGTTTATCTCGGTTCTTCTGTTGAAGACGATGCTGTTTATTGGGCCACTGATTTCTCTGGCGCGGATAATTTGGATGCGGAATTGGGTCAAAGGAAGCTCAATTTTATCGAGCTGCGAATGCTCATGGTAGCCACGAATTGGTCGGATACATGGGCAATGGGACAGTTGGCTATTGCTGGTCATGGCAGGGCTTTGCTAGATTGGCATAGAACATCACGTTTCTGTGGGCATTGTGGAGAAAGGACAGTCCCAATGGAAGCTGGGAAGCGAAGGGAATGCTCCAATCAGTCATGCAAGTTGAGGATTTTCCCTCGTGTCGATCCTGTTGTTATCATGTTAGTCATTGACCAGGAGAACGACCGCGCACTCTTAAGCAGGCAATCGAGATATGTGCCCCGAAAATGGAGTTGCTTAGCTGGTTTTATGGAGCCATGGCCAGGTCTGTTCATTCTGAAAGTACAGCTTCTTAACCACGCCTTTGCACTATCTGTTGTTGGACGAAGCACAATGCCGTGTCAATTGATGGTTGGGTTCTTTGCGTATGCAAAATCGCTCGATATCAAGGTGGACAAGGCTGAGTTAGAAGACGCCCAATGGCACACTAGAGAAGATGTGAAGAAAGCTTTAACATTAAGCGACTACGAGAAGACGCAAAGATCTGCAGCAGCCAAAGTCGAACAACTGTGCAACGGAGTTGAAAGCAGACACAGTCAGTCTGCTGATATCAACGCTGAAGATGGTGGACTTGCTTCCATGTTCTTTCCTGGCCCTTATGCAATCGCTCATCAACTTATCTCCTCTTGGGCTTATCAAGACAATGAAAAATGA

Protein sequence

MANGARTFLTVSKSTSRLLRLPPASSRFFTPFNSSCNGATFCSSTGTPSMSINLQSHAFARNPIRSKTPNPENPLSPDASFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSNGGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAELGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPGLFILKVQLLNHAFALSVVGRSTMPCQLMVGFFAYAKSLDIKVDKAELEDAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFPGPYAIAHQLISSWAYQDNEK

Homology

[Close]

BLAST of Carg12148-RA vs. NCBI nr
Match: KAG7021802.1 (Nudix hydrolase 19, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 866.7 bits (2238), Expect = 8.4e-248
Identity = 428/428 (100.00%), Postives = 428/428 (100.00%), Query Frame = 0


Query: 1   MANGARTFLTVSKSTSRLLRLPPASSRFFTPFNSSCNGATFCSSTGTPSMSINLQSHAFA 60
           MANGARTFLTVSKSTSRLLRLPPASSRFFTPFNSSCNGATFCSSTGTPSMSINLQSHAFA
Sbjct: 1   MANGARTFLTVSKSTSRLLRLPPASSRFFTPFNSSCNGATFCSSTGTPSMSINLQSHAFA 60

Query: 61  RNPIRSKTPNPENPLSPDASFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSN 120
           RNPIRSKTPNPENPLSPDASFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSN
Sbjct: 61  RNPIRSKTPNPENPLSPDASFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSN 120

Query: 121 GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE 180
           GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE
Sbjct: 121 GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE 180

Query: 181 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA 240
           LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA
Sbjct: 181 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA 240

Query: 241 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPG 300
           GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPG
Sbjct: 241 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPG 300

Query: 301 LFILKVQLLNHAFALSVVGRSTMPCQLMVGFFAYAKSLDIKVDKAELEDAQWHTREDVKK 360
           LFILKVQLLNHAFALSVVGRSTMPCQLMVGFFAYAKSLDIKVDKAELEDAQWHTREDVKK
Sbjct: 301 LFILKVQLLNHAFALSVVGRSTMPCQLMVGFFAYAKSLDIKVDKAELEDAQWHTREDVKK 360

Query: 361 ALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFPGPYAIAHQLISS 420
           ALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFPGPYAIAHQLISS
Sbjct: 361 ALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFPGPYAIAHQLISS 420

Query: 421 WAYQDNEK 429
           WAYQDNEK
Sbjct: 421 WAYQDNEK 428

[Close]

BLAST of Carg12148-RA vs. NCBI nr
Match: KAG6587917.1 (Nudix hydrolase 19, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 805.4 bits (2079), Expect = 2.3e-229
Identity = 406/440 (92.27%), Postives = 412/440 (93.64%), Query Frame = 0


Query: 1   MANGARTFLTVSKSTSRLLRLPPASSRFFTPFNSSCNGATFCSSTGTPSMSINLQSHAFA 60
           MANGARTFLTVSKSTSRLLRLPPASSRFF PFNSS NGATFCSSTGTPSMSINLQSHAFA
Sbjct: 45  MANGARTFLTVSKSTSRLLRLPPASSRFFPPFNSSFNGATFCSSTGTPSMSINLQSHAFA 104

Query: 61  RNPIRSKTPNPENPLSPDASFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSN 120
           RNPIRSKTP PENPLSPD+SFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSN
Sbjct: 105 RNPIRSKTPKPENPLSPDSSFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSN 164

Query: 121 GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE 180
           GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE
Sbjct: 165 GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE 224

Query: 181 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA 240
           LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA
Sbjct: 225 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA 284

Query: 241 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPG 300
           GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEP   
Sbjct: 285 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPGES 344

Query: 301 LFILKV------------QLLNHAFALSVVGRSTMPCQLMVGFFAYAKSLDIKVDKAELE 360
           L    +            +++ H+     VGRSTMPCQLMVGFFAYAKSLDIKVDKAELE
Sbjct: 345 LEEAVIRETLEETGIEVGKVVYHSSQPWPVGRSTMPCQLMVGFFAYAKSLDIKVDKAELE 404

Query: 361 DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFP 420
           DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFP
Sbjct: 405 DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFP 464

Query: 421 GPYAIAHQLISSWAYQDNEK 429
           GPYAIAHQLISSWAYQDNEK
Sbjct: 465 GPYAIAHQLISSWAYQDNEK 484

[Close]

BLAST of Carg12148-RA vs. NCBI nr
Match: XP_022931635.1 (nudix hydrolase 19, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 800.0 bits (2065), Expect = 9.7e-228
Identity = 403/440 (91.59%), Postives = 410/440 (93.18%), Query Frame = 0


Query: 1   MANGARTFLTVSKSTSRLLRLPPASSRFFTPFNSSCNGATFCSSTGTPSMSINLQSHAFA 60
           MANGARTFLTVSKSTSRLL LPPASSRFF PFNSS NGATFCSSTGTPSMSINLQSHAFA
Sbjct: 45  MANGARTFLTVSKSTSRLLHLPPASSRFFPPFNSSFNGATFCSSTGTPSMSINLQSHAFA 104

Query: 61  RNPIRSKTPNPENPLSPDASFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSN 120
           RNPIRSKTP PENPLSPD+SFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGK N
Sbjct: 105 RNPIRSKTPKPENPLSPDSSFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKRN 164

Query: 121 GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE 180
           GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE
Sbjct: 165 GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE 224

Query: 181 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA 240
           LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA
Sbjct: 225 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA 284

Query: 241 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPG 300
           GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEP   
Sbjct: 285 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPGES 344

Query: 301 LFILKV------------QLLNHAFALSVVGRSTMPCQLMVGFFAYAKSLDIKVDKAELE 360
           L    +            +++ H+     VGRSTMPCQLMVGFFAYAKSLDIKVDKAELE
Sbjct: 345 LEEAVIRETLEETGIEVGKVVYHSSQPWPVGRSTMPCQLMVGFFAYAKSLDIKVDKAELE 404

Query: 361 DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFP 420
           DAQWHTREDVKKALTLSDY+KTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFP
Sbjct: 405 DAQWHTREDVKKALTLSDYKKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFP 464

Query: 421 GPYAIAHQLISSWAYQDNEK 429
           GPYAIAHQLISSWAYQDNEK
Sbjct: 465 GPYAIAHQLISSWAYQDNEK 484

[Close]

BLAST of Carg12148-RA vs. NCBI nr
Match: XP_023530992.1 (nudix hydrolase 19, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 791.6 bits (2043), Expect = 3.4e-225
Identity = 401/440 (91.14%), Postives = 407/440 (92.50%), Query Frame = 0


Query: 1   MANGARTFLTVSKSTSRLLRLPPASSRFFTPFNSSCNGATFCSSTGTPSMSINLQSHAFA 60
           MANGARTFLTVSKSTSRLLRLPPASSRFF PFNSS NGATFCSSTGTPSMSINLQSHAFA
Sbjct: 45  MANGARTFLTVSKSTSRLLRLPPASSRFFPPFNSSFNGATFCSSTGTPSMSINLQSHAFA 104

Query: 61  RNPIRSKTPNPENPLSPDASFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSN 120
           RNPIRSKTP P NPLSPD+SFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSN
Sbjct: 105 RNPIRSKTPKPGNPLSPDSSFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSN 164

Query: 121 GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE 180
           GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFS ADNLDAE
Sbjct: 165 GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSVADNLDAE 224

Query: 181 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA 240
           LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTS FCGHCGERTVPMEA
Sbjct: 225 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSGFCGHCGERTVPMEA 284

Query: 241 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPG 300
           GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEP   
Sbjct: 285 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPGES 344

Query: 301 LFILKV------------QLLNHAFALSVVGRSTMPCQLMVGFFAYAKSLDIKVDKAELE 360
           L    +            +++ H+     VGRSTMPCQLMVGFFAYAKSLDIKVDKAELE
Sbjct: 345 LEEAVIRETLEETGIEVGKVVYHSSQPWPVGRSTMPCQLMVGFFAYAKSLDIKVDKAELE 404

Query: 361 DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFP 420
           DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAED GLASMFFP
Sbjct: 405 DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAEDSGLASMFFP 464

Query: 421 GPYAIAHQLISSWAYQDNEK 429
           GPYAIAHQLISSWAYQDN K
Sbjct: 465 GPYAIAHQLISSWAYQDNGK 484

[Close]

BLAST of Carg12148-RA vs. NCBI nr
Match: XP_023006376.1 (nudix hydrolase 19, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 786.6 bits (2030), Expect = 1.1e-223
Identity = 398/440 (90.45%), Postives = 406/440 (92.27%), Query Frame = 0


Query: 1   MANGARTFLTVSKSTSRLLRLPPASSRFFTPFNSSCNGATFCSSTGTPSMSINLQSHAFA 60
           MANGAR FLTVSKSTSRLLRLPPASSRFF PFNSS NGATFCSSTGTPSMSINLQSHAFA
Sbjct: 1   MANGARAFLTVSKSTSRLLRLPPASSRFFPPFNSSFNGATFCSSTGTPSMSINLQSHAFA 60

Query: 61  RNPIRSKTPNPENPLSPDASFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSN 120
           RNPIRSKTP PENPLSPD+SFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGK N
Sbjct: 61  RNPIRSKTPKPENPLSPDSSFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKRN 120

Query: 121 GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE 180
           GGLASGWGLGWISLADC SLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE
Sbjct: 121 GGLASGWGLGWISLADCASLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE 180

Query: 181 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA 240
           LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHC ERTVPMEA
Sbjct: 181 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCRERTVPMEA 240

Query: 241 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPG 300
           GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEP   
Sbjct: 241 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPGES 300

Query: 301 LFILKV------------QLLNHAFALSVVGRSTMPCQLMVGFFAYAKSLDIKVDKAELE 360
           L    +            +++ H+     VGRSTMPCQLMVGFFAYAKSLDIKVDKAELE
Sbjct: 301 LEEAVIRETLEETGIEVGKVVYHSSQPWPVGRSTMPCQLMVGFFAYAKSLDIKVDKAELE 360

Query: 361 DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFP 420
           DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCN VESRHSQSADINAEDGGLASMFFP
Sbjct: 361 DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNRVESRHSQSADINAEDGGLASMFFP 420

Query: 421 GPYAIAHQLISSWAYQDNEK 429
           GP+AIAHQLISSWAYQD+ K
Sbjct: 421 GPHAIAHQLISSWAYQDDGK 440

[Close]

BLAST of Carg12148-RA vs. ExPASy Swiss-Prot
Match: Q94A82 (Nudix hydrolase 19, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT19 PE=1 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 1.4e-123
Identity = 228/390 (58.46%), Postives = 284/390 (72.82%), Query Frame = 0


Query: 49  SMSINLQSHAFARNPIRSKTPNPENPLSPDASFESLKSKLLAKTPLLS--SPDFKILPFR 108
           +MS+NL++HAFA NP++SKTP   +P SP ++FESLK+ L+   P  S  SPDFK+LPF 
Sbjct: 35  TMSMNLKTHAFAGNPLKSKTPKSTDPFSPTSAFESLKT-LIPVIPNHSTPSPDFKVLPFS 94

Query: 109 KGWPLVSCNGGKSNGGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVY 168
           KG PLV  +GG +N      W LGW+SLADC+ LLA  G  + ED LVYLG  +E+D VY
Sbjct: 95  KGRPLVFSSGGDAN--TTPIWHLGWVSLADCKVLLASCGVDLNEDSLVYLGPKLEEDLVY 154

Query: 169 WATDFSGADNLDAELGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSR 228
           WA D +  D   +ELG RKL F+ELR LMVA +W+D  AM +LAIAG+ RALL+WH  S+
Sbjct: 155 WAVDLA-EDGFVSELGGRKLCFVELRTLMVAADWADQRAMDELAIAGNARALLEWHNVSQ 214

Query: 229 FCGHCGERTVPMEAGKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPR 288
           FCG CG +T P EAG+R++CS+++C+ R++PRVDPVVIMLVID+ENDRALLSRQSRYVPR
Sbjct: 215 FCGSCGSKTFPKEAGRRKQCSDETCRKRVYPRVDPVVIMLVIDRENDRALLSRQSRYVPR 274

Query: 289 KWSCLAGFMEP------------WPGLFILKVQLLNHAFALSVVGRSTMPCQLMVGFFAY 348
            WSCLAGF+EP            W    I    ++ H+     VG S+MPCQLM+GFFA+
Sbjct: 275 MWSCLAGFIEPGESLEEAVRRETWEETGIEVGDVVYHSSQPWPVGPSSMPCQLMLGFFAF 334

Query: 349 AKSLDIKVDKAELEDAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSAD 408
           AK+LDI VDK ELEDAQWH+RE+VKKAL +++Y K QR+AAAKVEQ+C GVE   S S D
Sbjct: 335 AKTLDINVDKEELEDAQWHSREEVKKALAVAEYRKAQRTAAAKVEQICKGVERSQSLSTD 394

Query: 409 INAEDGGLASMFFPGPYAIAHQLISSWAYQ 425
            N E G LA MF PGP+AIAH LIS+W  Q
Sbjct: 395 FNLESGELAPMFIPGPFAIAHHLISAWVNQ 420

[Close]

BLAST of Carg12148-RA vs. ExPASy Swiss-Prot
Match: Q4R7L8 (NAD-capped RNA hydrolase NUDT12 OS=Macaca fascicularis OX=9541 GN=NUDT12 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.4e-24
Identity = 96/292 (32.88%), Postives = 122/292 (41.78%), Query Frame = 0


Query: 147 KVTEDLLVYLGS---SVEDDAVYWATDFSGADNLDA-ELGQRKLNFIELRMLMVATNWSD 206
           ++ + LL Y G      ED  V W     G D + A E  QR  N   L   M A     
Sbjct: 204 EMKDKLLNYAGEVPREEEDGLVAWFA--LGIDPIAAEEFKQRHENCYFLHPPMPALLQLK 263

Query: 207 TWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKL------RIF 266
               G +A A   R++L WH   +FC  CG  T   E G +R C  + C          +
Sbjct: 264 EKEAGVVAQA---RSVLAWHSRYKFCPTCGNGTKIEEGGYKRVCLKEDCPSLNGVHNTSY 323

Query: 267 PRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPGLFILKVQLLNHAFALS 326
           PRVDPVVIM VI  +  R LL RQ R+ P  ++CLAGF+EP   +     + +     + 
Sbjct: 324 PRVDPVVIMQVIHPDGTRCLLGRQKRFPPGMFTCLAGFIEPGETIEDAVRREVEEESGVK 383

Query: 327 VVGRSTMPCQ-------LMVGFFAYAKSLDIKVDKAELEDAQWHTREDVKKALTLSDYEK 386
           V     + CQ       LM+G  A A S +IKVDK E+EDA+W TRE V   LT      
Sbjct: 384 VGHVQYVSCQPWPMPSSLMIGCLAVAVSTEIKVDKNEIEDARWFTREQVLDVLT------ 443

Query: 387 TQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFPGPYAIAHQLISSW 422
                                         G   + F P   AIAHQLI  W
Sbjct: 444 -----------------------------KGKQQAFFVPPSRAIAHQLIKHW 455

[Close]

BLAST of Carg12148-RA vs. ExPASy Swiss-Prot
Match: Q9BQG2 (NAD-capped RNA hydrolase NUDT12 OS=Homo sapiens OX=9606 GN=NUDT12 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.9e-24
Identity = 95/292 (32.53%), Postives = 122/292 (41.78%), Query Frame = 0


Query: 147 KVTEDLLVYLGS---SVEDDAVYWATDFSGADNLDA-ELGQRKLNFIELRMLMVATNWSD 206
           ++ + LL Y G      ED  V W     G D + A E  QR  N   L   M A     
Sbjct: 204 EIKDKLLNYAGEVPREEEDGLVAWFA--LGIDPIAAEEFKQRHENCYFLHPPMPALLQLK 263

Query: 207 TWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKL------RIF 266
               G +A A   R++L WH   +FC  CG  T   E G +R C  + C          +
Sbjct: 264 EKEAGVVAQA---RSVLAWHSRYKFCPTCGNATKIEEGGYKRLCLKEDCPSLNGVHNTSY 323

Query: 267 PRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPGLFILKVQLLNHAFALS 326
           PRVDPVVIM VI  +  + LL RQ R+ P  ++CLAGF+EP   +     + +     + 
Sbjct: 324 PRVDPVVIMQVIHPDGTKCLLGRQKRFPPGMFTCLAGFIEPGETIEDAVRREVEEESGVK 383

Query: 327 VVGRSTMPCQ-------LMVGFFAYAKSLDIKVDKAELEDAQWHTREDVKKALTLSDYEK 386
           V     + CQ       LM+G  A A S +IKVDK E+EDA+W TRE V   LT      
Sbjct: 384 VGHVQYVACQPWPMPSSLMIGCLALAVSTEIKVDKNEIEDARWFTREQVLDVLT------ 443

Query: 387 TQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFPGPYAIAHQLISSW 422
                                         G   + F P   AIAHQLI  W
Sbjct: 444 -----------------------------KGKQQAFFVPPSRAIAHQLIKHW 455

[Close]

BLAST of Carg12148-RA vs. ExPASy Swiss-Prot
Match: Q9DCN1 (NAD-capped RNA hydrolase NUDT12 OS=Mus musculus OX=10090 GN=Nudt12 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 9.3e-24
Identity = 74/227 (32.60%), Postives = 99/227 (43.61%), Query Frame = 0


Query: 208 QLAIAGHGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKL------RIFPRVDP 267
           +  +    R++L WH   +FC  CG  T   E G +R C  ++C          +PRVDP
Sbjct: 264 EAGVVAQARSVLAWHSRYKFCPTCGSATKIEEGGYKRVCVRETCPSLQGVHNTSYPRVDP 323

Query: 268 VVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPGLFILKVQLLNHAFALSVVGRS 327
           VVIM VI  +  + LL RQ R+ P  ++CLAGF+EP   +     + +     + V    
Sbjct: 324 VVIMQVIHPDGTKCLLGRQKRFPPGMFTCLAGFIEPGETIEDAVRREVEEESGVKVGHVQ 383

Query: 328 TMPCQ-------LMVGFFAYAKSLDIKVDKAELEDAQWHTREDVKKALTLSDYEKTQRSA 387
            + CQ       LM+G  A A S +IKVDK E+EDA+W TRE V   LT           
Sbjct: 384 YVSCQPWPMPSSLMIGCLAVAVSTEIKVDKNEIEDARWFTREQVVDVLT----------- 443

Query: 388 AAKVEQLCNGVESRHSQSADINAEDGGLASMFFPGPYAIAHQLISSW 422
                                    G   + F P   AIAHQLI  W
Sbjct: 444 ------------------------KGKQQAFFVPPSRAIAHQLIKHW 455

[Close]

BLAST of Carg12148-RA vs. ExPASy Swiss-Prot
Match: Q29RH3 (NAD-capped RNA hydrolase NUDT12 OS=Bos taurus OX=9913 GN=NUDT12 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.2e-23
Identity = 74/227 (32.60%), Postives = 98/227 (43.17%), Query Frame = 0


Query: 208 QLAIAGHGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKL------RIFPRVDP 267
           +  +    R++L WH   +FC  CG  T   E G +R C  + C          +PRVDP
Sbjct: 246 EAGVVAQARSVLAWHSRYKFCPTCGNATKIEEGGYKRVCLKEDCPSLHGVHNTSYPRVDP 305

Query: 268 VVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPGLFILKVQLLNHAFALSVVGRS 327
           VVIM VI  +  + LL RQ R+ P  ++CLAGF+EP   +     + +     + V    
Sbjct: 306 VVIMQVIHPDGTKCLLGRQKRFPPGMFTCLAGFIEPGETIEDAVRREVEEESGVKVGHVQ 365

Query: 328 TMPCQ-------LMVGFFAYAKSLDIKVDKAELEDAQWHTREDVKKALTLSDYEKTQRSA 387
            + CQ       LM+G  A A S +IKVDK E+EDA+W TRE V   LT           
Sbjct: 366 YVSCQPWPMPSSLMIGCLAVAVSTEIKVDKNEIEDARWFTREQVVDVLT----------- 425

Query: 388 AAKVEQLCNGVESRHSQSADINAEDGGLASMFFPGPYAIAHQLISSW 422
                                    G   + F P   AIAHQLI  W
Sbjct: 426 ------------------------KGKQQAFFVPPSRAIAHQLIKHW 437

[Close]

BLAST of Carg12148-RA vs. ExPASy TrEMBL
Match: A0A6J1F012 (NAD(+) diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111437793 PE=4 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 4.7e-228
Identity = 403/440 (91.59%), Postives = 410/440 (93.18%), Query Frame = 0


Query: 1   MANGARTFLTVSKSTSRLLRLPPASSRFFTPFNSSCNGATFCSSTGTPSMSINLQSHAFA 60
           MANGARTFLTVSKSTSRLL LPPASSRFF PFNSS NGATFCSSTGTPSMSINLQSHAFA
Sbjct: 45  MANGARTFLTVSKSTSRLLHLPPASSRFFPPFNSSFNGATFCSSTGTPSMSINLQSHAFA 104

Query: 61  RNPIRSKTPNPENPLSPDASFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSN 120
           RNPIRSKTP PENPLSPD+SFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGK N
Sbjct: 105 RNPIRSKTPKPENPLSPDSSFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKRN 164

Query: 121 GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE 180
           GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE
Sbjct: 165 GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE 224

Query: 181 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA 240
           LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA
Sbjct: 225 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA 284

Query: 241 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPG 300
           GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEP   
Sbjct: 285 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPGES 344

Query: 301 LFILKV------------QLLNHAFALSVVGRSTMPCQLMVGFFAYAKSLDIKVDKAELE 360
           L    +            +++ H+     VGRSTMPCQLMVGFFAYAKSLDIKVDKAELE
Sbjct: 345 LEEAVIRETLEETGIEVGKVVYHSSQPWPVGRSTMPCQLMVGFFAYAKSLDIKVDKAELE 404

Query: 361 DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFP 420
           DAQWHTREDVKKALTLSDY+KTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFP
Sbjct: 405 DAQWHTREDVKKALTLSDYKKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFP 464

Query: 421 GPYAIAHQLISSWAYQDNEK 429
           GPYAIAHQLISSWAYQDNEK
Sbjct: 465 GPYAIAHQLISSWAYQDNEK 484

[Close]

BLAST of Carg12148-RA vs. ExPASy TrEMBL
Match: A0A6J1KXL6 (NAD(+) diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111499124 PE=4 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 5.4e-224
Identity = 398/440 (90.45%), Postives = 406/440 (92.27%), Query Frame = 0


Query: 1   MANGARTFLTVSKSTSRLLRLPPASSRFFTPFNSSCNGATFCSSTGTPSMSINLQSHAFA 60
           MANGAR FLTVSKSTSRLLRLPPASSRFF PFNSS NGATFCSSTGTPSMSINLQSHAFA
Sbjct: 1   MANGARAFLTVSKSTSRLLRLPPASSRFFPPFNSSFNGATFCSSTGTPSMSINLQSHAFA 60

Query: 61  RNPIRSKTPNPENPLSPDASFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKSN 120
           RNPIRSKTP PENPLSPD+SFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGK N
Sbjct: 61  RNPIRSKTPKPENPLSPDSSFESLKSKLLAKTPLLSSPDFKILPFRKGWPLVSCNGGKRN 120

Query: 121 GGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE 180
           GGLASGWGLGWISLADC SLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE
Sbjct: 121 GGLASGWGLGWISLADCASLLALSGSKVTEDLLVYLGSSVEDDAVYWATDFSGADNLDAE 180

Query: 181 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCGERTVPMEA 240
           LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHC ERTVPMEA
Sbjct: 181 LGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSRFCGHCRERTVPMEA 240

Query: 241 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPWPG 300
           GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEP   
Sbjct: 241 GKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPRKWSCLAGFMEPGES 300

Query: 301 LFILKV------------QLLNHAFALSVVGRSTMPCQLMVGFFAYAKSLDIKVDKAELE 360
           L    +            +++ H+     VGRSTMPCQLMVGFFAYAKSLDIKVDKAELE
Sbjct: 301 LEEAVIRETLEETGIEVGKVVYHSSQPWPVGRSTMPCQLMVGFFAYAKSLDIKVDKAELE 360

Query: 361 DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSADINAEDGGLASMFFP 420
           DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCN VESRHSQSADINAEDGGLASMFFP
Sbjct: 361 DAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNRVESRHSQSADINAEDGGLASMFFP 420

Query: 421 GPYAIAHQLISSWAYQDNEK 429
           GP+AIAHQLISSWAYQD+ K
Sbjct: 421 GPHAIAHQLISSWAYQDDGK 440

[Close]

BLAST of Carg12148-RA vs. ExPASy TrEMBL
Match: A0A5A7TZD4 (NAD(+) diphosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G00380 PE=4 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.4e-195
Identity = 353/467 (75.59%), Postives = 384/467 (82.23%), Query Frame = 0


Query: 1   MANGARTFLTVSKSTSRLLRLPPAS---------------------------SRFFTPFN 60
           MAN ART +TVSKST RLLRLPP+S                           SRF +PFN
Sbjct: 1   MANSARTIVTVSKSTCRLLRLPPSSASINLHTPFFLSYPPPPNPNTRNGIPISRFCSPFN 60

Query: 61  SSCNGATFCSSTGTPSMSINLQSHAFARNPIRSKTPNPENPLSPDASFESLKSKLLAKTP 120
           +S +G TF SST TPSMSINLQSHAFARNPIRSKTP P +PL PD+SFESLK+KLL KTP
Sbjct: 61  TSFHGPTFSSSTRTPSMSINLQSHAFARNPIRSKTPKPGHPLFPDSSFESLKTKLLTKTP 120

Query: 121 LLSSPDFKILPFRKGWPLVSCNGGKSNGGLASGWGLGWISLADCESLLALSGSKVTEDLL 180
           L+SSPDFKILPF+KGWPLVSCNGGK NG  ASGWGLGWISLADC+SLLAL GS++TEDL 
Sbjct: 121 LISSPDFKILPFKKGWPLVSCNGGKGNGDSASGWGLGWISLADCKSLLALRGSQLTEDLF 180

Query: 181 VYLGSSVEDDAVYWATDFSGADNLDAELGQRKLNFIELRMLMVATNWSDTWAMGQLAIAG 240
           VYLGSSVEDDAVYWA DFSG D+LD ELG+++L+FIELR LMVATNWSD+WAMGQLA+AG
Sbjct: 181 VYLGSSVEDDAVYWAIDFSGEDDLDEELGRKQLSFIELRTLMVATNWSDSWAMGQLAVAG 240

Query: 241 HGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKLRIFPRVDPVVIMLVIDQEND 300
           HGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKLRIFPRVDPVVIMLVIDQEND
Sbjct: 241 HGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKLRIFPRVDPVVIMLVIDQEND 300

Query: 301 RALLSRQSRYVPRKWSCLAGFMEPWPGLFILKV------------QLLNHAFALSVVGRS 360
           RALLSRQSRYVPRKWSCLAGFMEP   L    +            +++ H+     VGRS
Sbjct: 301 RALLSRQSRYVPRKWSCLAGFMEPGESLEEAVIRETLEETGIEVGEVVYHSSQPWPVGRS 360

Query: 361 TMPCQLMVGFFAYAKSLDIKVDKAELEDAQWHTREDVKKALTLSDYEKTQRSAAAKVEQL 420
           TMPCQLMVGFFAYAKSLDI+VDKAELEDAQWH REDVK+ALTLS+YEKTQR+AAAKVEQ+
Sbjct: 361 TMPCQLMVGFFAYAKSLDIRVDKAELEDAQWHKREDVKRALTLSEYEKTQRTAAAKVEQM 420

Query: 421 CNGVESRHSQSADINAEDGGLASMFFPGPYAIAHQLISSWAYQDNEK 429
           C G E+RH  S D NAED GLASM FPGPYAIAHQLISSWAYQDN K
Sbjct: 421 CKGAETRH--SGDFNAEDSGLASMLFPGPYAIAHQLISSWAYQDNGK 465

[Close]

BLAST of Carg12148-RA vs. ExPASy TrEMBL
Match: A0A1S3CNB6 (NAD(+) diphosphatase OS=Cucumis melo OX=3656 GN=LOC103502742 PE=4 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.4e-195
Identity = 353/467 (75.59%), Postives = 384/467 (82.23%), Query Frame = 0


Query: 1   MANGARTFLTVSKSTSRLLRLPPAS---------------------------SRFFTPFN 60
           MAN ART +TVSKST RLLRLPP+S                           SRF +PFN
Sbjct: 1   MANSARTIVTVSKSTCRLLRLPPSSASINLHTPFFLSYPPPPNPNTRNGIPISRFCSPFN 60

Query: 61  SSCNGATFCSSTGTPSMSINLQSHAFARNPIRSKTPNPENPLSPDASFESLKSKLLAKTP 120
           +S +G TF SST TPSMSINLQSHAFARNPIRSKTP P +PL PD+SFESLK+KLL KTP
Sbjct: 61  TSFHGPTFSSSTRTPSMSINLQSHAFARNPIRSKTPKPGHPLFPDSSFESLKTKLLTKTP 120

Query: 121 LLSSPDFKILPFRKGWPLVSCNGGKSNGGLASGWGLGWISLADCESLLALSGSKVTEDLL 180
           L+SSPDFKILPF+KGWPLVSCNGGK NG  ASGWGLGWISLADC+SLLAL GS++TEDL 
Sbjct: 121 LISSPDFKILPFKKGWPLVSCNGGKGNGDSASGWGLGWISLADCKSLLALRGSQLTEDLF 180

Query: 181 VYLGSSVEDDAVYWATDFSGADNLDAELGQRKLNFIELRMLMVATNWSDTWAMGQLAIAG 240
           VYLGSSVEDDAVYWA DFSG D+LD ELG+++L+FIELR LMVATNWSD+WAMGQLA+AG
Sbjct: 181 VYLGSSVEDDAVYWAIDFSGEDDLDEELGRKQLSFIELRTLMVATNWSDSWAMGQLAVAG 240

Query: 241 HGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKLRIFPRVDPVVIMLVIDQEND 300
           HGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKLRIFPRVDPVVIMLVIDQEND
Sbjct: 241 HGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKLRIFPRVDPVVIMLVIDQEND 300

Query: 301 RALLSRQSRYVPRKWSCLAGFMEPWPGLFILKV------------QLLNHAFALSVVGRS 360
           RALLSRQSRYVPRKWSCLAGFMEP   L    +            +++ H+     VGRS
Sbjct: 301 RALLSRQSRYVPRKWSCLAGFMEPGESLEEAVIRETLEETGIEVGEVVYHSSQPWPVGRS 360

Query: 361 TMPCQLMVGFFAYAKSLDIKVDKAELEDAQWHTREDVKKALTLSDYEKTQRSAAAKVEQL 420
           TMPCQLMVGFFAYAKSLDI+VDKAELEDAQWH REDVK+ALTLS+YEKTQR+AAAKVEQ+
Sbjct: 361 TMPCQLMVGFFAYAKSLDIRVDKAELEDAQWHKREDVKRALTLSEYEKTQRTAAAKVEQM 420

Query: 421 CNGVESRHSQSADINAEDGGLASMFFPGPYAIAHQLISSWAYQDNEK 429
           C G E+RH  S D NAED GLASM FPGPYAIAHQLISSWAYQDN K
Sbjct: 421 CKGAETRH--SGDFNAEDSGLASMLFPGPYAIAHQLISSWAYQDNGK 465

[Close]

BLAST of Carg12148-RA vs. ExPASy TrEMBL
Match: A0A5D3E183 (NAD(+) diphosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold64G00440 PE=4 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 5.2e-195
Identity = 353/467 (75.59%), Postives = 384/467 (82.23%), Query Frame = 0


Query: 1   MANGARTFLTVSKSTSRLLRLPPAS---------------------------SRFFTPFN 60
           MAN ART +TVSKST RLLRLPP+S                           SRF +PFN
Sbjct: 1   MANSARTIVTVSKSTCRLLRLPPSSASINLHSPFFLSYPPPPNPNTRNGIPISRFCSPFN 60

Query: 61  SSCNGATFCSSTGTPSMSINLQSHAFARNPIRSKTPNPENPLSPDASFESLKSKLLAKTP 120
           +S +G TF SST TPSMSINLQSHAFARNPIRSKTP P +PL PD+SFESLK+KLL KTP
Sbjct: 61  TSFHGPTFSSSTRTPSMSINLQSHAFARNPIRSKTPKPGHPLFPDSSFESLKTKLLTKTP 120

Query: 121 LLSSPDFKILPFRKGWPLVSCNGGKSNGGLASGWGLGWISLADCESLLALSGSKVTEDLL 180
           L+SSPDFKILPF+KGWPLVSCNG K NG  ASGWGLGWISLADC+SLLALSGS++TEDL 
Sbjct: 121 LISSPDFKILPFKKGWPLVSCNGLKGNGDSASGWGLGWISLADCKSLLALSGSQLTEDLF 180

Query: 181 VYLGSSVEDDAVYWATDFSGADNLDAELGQRKLNFIELRMLMVATNWSDTWAMGQLAIAG 240
           VYLGSSVEDDAVYWA DFSG D+LD ELG+++L+FIELR LMVATNWSD+WAMGQLA+AG
Sbjct: 181 VYLGSSVEDDAVYWAIDFSGEDDLDEELGRKQLSFIELRTLMVATNWSDSWAMGQLAVAG 240

Query: 241 HGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKLRIFPRVDPVVIMLVIDQEND 300
           HGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKLRIFPRVDPVVIMLVIDQEND
Sbjct: 241 HGRALLDWHRTSRFCGHCGERTVPMEAGKRRECSNQSCKLRIFPRVDPVVIMLVIDQEND 300

Query: 301 RALLSRQSRYVPRKWSCLAGFMEPWPGLFILKV------------QLLNHAFALSVVGRS 360
           RALLSRQSRYVPRKWSCLAGFMEP   L    +            +++ H+     VGRS
Sbjct: 301 RALLSRQSRYVPRKWSCLAGFMEPGESLEEAVIRETLEETGIEVGEVVYHSSQPWPVGRS 360

Query: 361 TMPCQLMVGFFAYAKSLDIKVDKAELEDAQWHTREDVKKALTLSDYEKTQRSAAAKVEQL 420
           TMPCQLMVGFFAYAKSLDI+VDKAELEDAQWH REDVK+ALTLS+YEKTQR+AAAKVEQ+
Sbjct: 361 TMPCQLMVGFFAYAKSLDIRVDKAELEDAQWHKREDVKRALTLSEYEKTQRTAAAKVEQM 420

Query: 421 CNGVESRHSQSADINAEDGGLASMFFPGPYAIAHQLISSWAYQDNEK 429
           C G E+RH  S D NAED GLASM FPGPYAIAHQLISSWAYQDN K
Sbjct: 421 CKGAETRH--SGDFNAEDSGLASMLFPGPYAIAHQLISSWAYQDNGK 465

[Close]

BLAST of Carg12148-RA vs. TAIR 10
Match: AT5G20070.1 (nudix hydrolase homolog 19 )

HSP 1 Score: 444.5 bits (1142), Expect = 9.6e-125
Identity = 228/390 (58.46%), Postives = 284/390 (72.82%), Query Frame = 0


Query: 49  SMSINLQSHAFARNPIRSKTPNPENPLSPDASFESLKSKLLAKTPLLS--SPDFKILPFR 108
           +MS+NL++HAFA NP++SKTP   +P SP ++FESLK+ L+   P  S  SPDFK+LPF 
Sbjct: 35  TMSMNLKTHAFAGNPLKSKTPKSTDPFSPTSAFESLKT-LIPVIPNHSTPSPDFKVLPFS 94

Query: 109 KGWPLVSCNGGKSNGGLASGWGLGWISLADCESLLALSGSKVTEDLLVYLGSSVEDDAVY 168
           KG PLV  +GG +N      W LGW+SLADC+ LLA  G  + ED LVYLG  +E+D VY
Sbjct: 95  KGRPLVFSSGGDAN--TTPIWHLGWVSLADCKVLLASCGVDLNEDSLVYLGPKLEEDLVY 154

Query: 169 WATDFSGADNLDAELGQRKLNFIELRMLMVATNWSDTWAMGQLAIAGHGRALLDWHRTSR 228
           WA D +  D   +ELG RKL F+ELR LMVA +W+D  AM +LAIAG+ RALL+WH  S+
Sbjct: 155 WAVDLA-EDGFVSELGGRKLCFVELRTLMVAADWADQRAMDELAIAGNARALLEWHNVSQ 214

Query: 229 FCGHCGERTVPMEAGKRRECSNQSCKLRIFPRVDPVVIMLVIDQENDRALLSRQSRYVPR 288
           FCG CG +T P EAG+R++CS+++C+ R++PRVDPVVIMLVID+ENDRALLSRQSRYVPR
Sbjct: 215 FCGSCGSKTFPKEAGRRKQCSDETCRKRVYPRVDPVVIMLVIDRENDRALLSRQSRYVPR 274

Query: 289 KWSCLAGFMEP------------WPGLFILKVQLLNHAFALSVVGRSTMPCQLMVGFFAY 348
            WSCLAGF+EP            W    I    ++ H+     VG S+MPCQLM+GFFA+
Sbjct: 275 MWSCLAGFIEPGESLEEAVRRETWEETGIEVGDVVYHSSQPWPVGPSSMPCQLMLGFFAF 334

Query: 349 AKSLDIKVDKAELEDAQWHTREDVKKALTLSDYEKTQRSAAAKVEQLCNGVESRHSQSAD 408
           AK+LDI VDK ELEDAQWH+RE+VKKAL +++Y K QR+AAAKVEQ+C GVE   S S D
Sbjct: 335 AKTLDINVDKEELEDAQWHSREEVKKALAVAEYRKAQRTAAAKVEQICKGVERSQSLSTD 394

Query: 409 INAEDGGLASMFFPGPYAIAHQLISSWAYQ 425
            N E G LA MF PGP+AIAH LIS+W  Q
Sbjct: 395 FNLESGELAPMFIPGPFAIAHHLISAWVNQ 420

The following BLAST results are available for this feature:

BLAST of Carg12148-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021802.1	8.4e-248	100.00	Nudix hydrolase 19, chloroplastic, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6587917.1	2.3e-229	92.27	Nudix hydrolase 19, chloroplastic, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022931635.1	9.7e-228	91.59	nudix hydrolase 19, chloroplastic-like [Cucurbita moschata]	[more]
XP_023530992.1	3.4e-225	91.14	nudix hydrolase 19, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_023006376.1	1.1e-223	90.45	nudix hydrolase 19, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of Carg12148-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94A82	1.4e-123	58.46	Nudix hydrolase 19, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT19 PE=1...	[more]
Q4R7L8	1.4e-24	32.88	NAD-capped RNA hydrolase NUDT12 OS=Macaca fascicularis OX=9541 GN=NUDT12 PE=2 SV...	[more]
Q9BQG2	1.9e-24	32.53	NAD-capped RNA hydrolase NUDT12 OS=Homo sapiens OX=9606 GN=NUDT12 PE=1 SV=1	[more]
Q9DCN1	9.3e-24	32.60	NAD-capped RNA hydrolase NUDT12 OS=Mus musculus OX=10090 GN=Nudt12 PE=1 SV=1	[more]
Q29RH3	1.2e-23	32.60	NAD-capped RNA hydrolase NUDT12 OS=Bos taurus OX=9913 GN=NUDT12 PE=2 SV=1	[more]

BLAST of Carg12148-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F012	4.7e-228	91.59	NAD(+) diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111437793 PE=4 SV=1	[more]
A0A6J1KXL6	5.4e-224	90.45	NAD(+) diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111499124 PE=4 SV=1	[more]
A0A5A7TZD4	1.4e-195	75.59	NAD(+) diphosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216...	[more]
A0A1S3CNB6	1.4e-195	75.59	NAD(+) diphosphatase OS=Cucumis melo OX=3656 GN=LOC103502742 PE=4 SV=1	[more]
A0A5D3E183	5.2e-195	75.59	NAD(+) diphosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold64G...	[more]

BLAST of Carg12148-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20070.1	9.6e-125	58.46	nudix hydrolase homolog 19	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015375	NADH pyrophosphatase-like, N-terminal	PFAM	PF09296	NUDIX-like	coord: 146..221 e-value: 1.1E-7 score: 32.5
None	No IPR available	GENE3D	3.90.79.10	Nucleoside Triphosphate Pyrophosphohydrolase	coord: 300..424 e-value: 4.4E-5 score: 25.2
None	No IPR available	GENE3D	3.90.79.20		coord: 131..257 e-value: 6.8E-18 score: 67.1
None	No IPR available	PANTHER	PTHR42904	NUDIX HYDROLASE, NUDC SUBFAMILY	coord: 50..424
None	No IPR available	PANTHER	PTHR42904:SF7	NADH PYROPHOSPHATASE-LIKE, NUDIX HYDROLASE DOMAIN-LIKE PROTEIN-RELATED	coord: 50..424
IPR015797	NUDIX hydrolase-like domain superfamily	SUPERFAMILY	55811	Nudix	coord: 209..364

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg12148	Carg12148	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12148-RA:1	Carg12148-RA:1	exon
Carg12148-RA:2	Carg12148-RA:2	exon
Carg12148-RA:3	Carg12148-RA:3	exon
Carg12148-RA:4	Carg12148-RA:4	exon
Carg12148-RA:5	Carg12148-RA:5	exon
Carg12148-RA:6	Carg12148-RA:6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12148-RA:cds	Carg12148-RA:cds	CDS
Carg12148-RA:cds	Carg12148-RA:cds_2	CDS
Carg12148-RA:cds	Carg12148-RA:cds_3	CDS
Carg12148-RA:cds	Carg12148-RA:cds_4	CDS
Carg12148-RA:cds	Carg12148-RA:cds_5	CDS
Carg12148-RA:cds	Carg12148-RA:cds_6	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg12148-RA	Carg12148-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0000210	NAD+ diphosphatase activity