Carg09932-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09932-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Auxin-responsive protein
Location	Carg_Chr10: 2486748 .. 2487747 (+)
Sequence length	765
RNA-Seq Expression	Carg09932-RA
Synteny	Carg09932-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTATGGAGCTTCAGCTGAGTCTTGGTTTGTTCCCCACCAATCCCACTAAACCCTTCGACCTAAACACCCATCACCGCGCTCTCCCTTGCTTGGATTTCGACTCAATCAGCCGCAAAAAGCGCCCTCTCGATATCGACCCCTCTTCGGCATTTCGTCGAACACTTCCTCTGCTTCTCTGGAATGACCATCCCAATAATGGCGATGACGACCCTAAAAACCCCAATTCCGCCTCCTCCAATCATCCGTACGCTACTCCTACCCATTTTTCTCTCTTTAATTTCGAATTCCGCGGCTGAATTTGTTAATTTGATTGATTTAACAGAGACAATCCGGAGGAACAGGAAATTGAGCTTGTCGGATGGCCGCCGCTTAAAAAGAGGAGGAAGACACTTTTCGTACAGAAGCGCATCGGAAGGGCCGTAGCGATCAGGCGTCCGGCGGCGGAGAACGACGGCGGGGTTTACAGGGAATTGAATTCGAATAATTCCCGGTACGTGAAGGTGAAGATGGAGGGAGTGGGAATTGGTAGGAAGGTTGATATACGGCAACACCATTGTTTGGATGCGCTTAGAGCTACTTTGATGAACATGTTTGGTGAGCTGGTGTTGTTATCCCAATTAAGAATAGTTTATCCGGGTTACTGTGTACTGATTTTTGGGTTGTTTGGAAACAGATAAGCCGGATTCCGACGGCTACAACCTCACCTACCAGGATACCGACGGTGATTGGTTGCTTGCTGAGGACGTAACTTGGAGGTAAAGCCAAAGACTCACAAAAATAATAATATTTATATATTAGTTTAAAAAAATCTGAGTGGGTTTTGATTATGACAGAGATTTCATCCGAACCGTCCAACGGATCAAACTGGAAAAGAACGGCGGCTGATTAGGGGTTACCGACACCGGCGGTCAGAGAACCAGATGCTGTCTAATTAAATAGATTTTTTTTTTTTTTTAGCACTTTAATTTTTAACGACCGTATGAATGGGTTCTTACT

mRNA sequence

ATGGCTATGGAGCTTCAGCTGAGTCTTGGTTTGTTCCCCACCAATCCCACTAAACCCTTCGACCTAAACACCCATCACCGCGCTCTCCCTTGCTTGGATTTCGACTCAATCAGCCGCAAAAAGCGCCCTCTCGATATCGACCCCTCTTCGGCATTTCGTCGAACACTTCCTCTGCTTCTCTGGAATGACCATCCCAATAATGGCGATGACGACCCTAAAAACCCCAATTCCGCCTCCTCCAATCATCCAGACAATCCGGAGGAACAGGAAATTGAGCTTGTCGGATGGCCGCCGCTTAAAAAGAGGAGGAAGACACTTTTCGTACAGAAGCGCATCGGAAGGGCCGTAGCGATCAGGCGTCCGGCGGCGGAGAACGACGGCGGGGTTTACAGGGAATTGAATTCGAATAATTCCCGGTACGTGAAGGTGAAGATGGAGGGAGTGGGAATTGGTAGGAAGGTTGATATACGGCAACACCATTGTTTGGATGCGCTTAGAGCTACTTTGATGAACATGTTTGATAAGCCGGATTCCGACGGCTACAACCTCACCTACCAGGATACCGACGGTGATTGGTTGCTTGCTGAGGACGTAACTTGGAGAGATTTCATCCGAACCGTCCAACGGATCAAACTGGAAAAGAACGGCGGCTGATTAGGGGTTACCGACACCGGCGGTCAGAGAACCAGATGCTGTCTAATTAAATAGATTTTTTTTTTTTTTTAGCACTTTAATTTTTAACGACCGTATGAATGGGTTCTTACT

Coding sequence (CDS)

ATGGCTATGGAGCTTCAGCTGAGTCTTGGTTTGTTCCCCACCAATCCCACTAAACCCTTCGACCTAAACACCCATCACCGCGCTCTCCCTTGCTTGGATTTCGACTCAATCAGCCGCAAAAAGCGCCCTCTCGATATCGACCCCTCTTCGGCATTTCGTCGAACACTTCCTCTGCTTCTCTGGAATGACCATCCCAATAATGGCGATGACGACCCTAAAAACCCCAATTCCGCCTCCTCCAATCATCCAGACAATCCGGAGGAACAGGAAATTGAGCTTGTCGGATGGCCGCCGCTTAAAAAGAGGAGGAAGACACTTTTCGTACAGAAGCGCATCGGAAGGGCCGTAGCGATCAGGCGTCCGGCGGCGGAGAACGACGGCGGGGTTTACAGGGAATTGAATTCGAATAATTCCCGGTACGTGAAGGTGAAGATGGAGGGAGTGGGAATTGGTAGGAAGGTTGATATACGGCAACACCATTGTTTGGATGCGCTTAGAGCTACTTTGATGAACATGTTTGATAAGCCGGATTCCGACGGCTACAACCTCACCTACCAGGATACCGACGGTGATTGGTTGCTTGCTGAGGACGTAACTTGGAGAGATTTCATCCGAACCGTCCAACGGATCAAACTGGAAAAGAACGGCGGCTGA

Protein sequence

MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLLWNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRRPAAENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDGYNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKNGG

Homology

[Close]

BLAST of Carg09932-RA vs. NCBI nr
Match: KAG7023504.1 (Auxin-responsive protein IAA29, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 453.8 bits (1166), Expect = 8.6e-124
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL 60
           MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL
Sbjct: 1   MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL 60

Query: 61  WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR 120
           WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR
Sbjct: 61  WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR 120

Query: 121 PAAENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG 180
           PAAENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG
Sbjct: 121 PAAENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG 180

Query: 181 YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKNGG 218
           YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKNGG
Sbjct: 181 YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKNGG 217

[Close]

BLAST of Carg09932-RA vs. NCBI nr
Match: KAG6589834.1 (Auxin-responsive protein IAA29, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 451.1 bits (1159), Expect = 5.6e-123
Identity = 215/217 (99.08%), Postives = 216/217 (99.54%), Query Frame = 0


Query: 1   MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL 60
           MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRK+RPLDIDPSSAFRRTLPLLL
Sbjct: 1   MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRKRRPLDIDPSSAFRRTLPLLL 60

Query: 61  WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR 120
           WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR
Sbjct: 61  WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR 120

Query: 121 PAAENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG 180
           PA ENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG
Sbjct: 121 PATENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG 180

Query: 181 YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKNGG 218
           YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKNGG
Sbjct: 181 YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKNGG 217

[Close]

BLAST of Carg09932-RA vs. NCBI nr
Match: XP_022960765.1 (uncharacterized protein LOC111461467 isoform X2 [Cucurbita moschata])

HSP 1 Score: 444.9 bits (1143), Expect = 4.0e-121
Identity = 213/215 (99.07%), Postives = 213/215 (99.07%), Query Frame = 0


Query: 1   MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL 60
           MAMELQLSL LFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL
Sbjct: 1   MAMELQLSLALFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL 60

Query: 61  WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR 120
           WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR
Sbjct: 61  WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR 120

Query: 121 PAAENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG 180
           PA ENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG
Sbjct: 121 PAMENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG 180

Query: 181 YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKN 216
           YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKN
Sbjct: 181 YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKN 215

[Close]

BLAST of Carg09932-RA vs. NCBI nr
Match: XP_023515749.1 (uncharacterized protein LOC111779818 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 431.4 bits (1108), Expect = 4.6e-117
Identity = 205/215 (95.35%), Postives = 209/215 (97.21%), Query Frame = 0


Query: 1   MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL 60
           MAMELQLSL LFPTNPTKPFDLNTHHRALPC DFDSISRKKRPLDIDPSSAFRRTLPLLL
Sbjct: 1   MAMELQLSLALFPTNPTKPFDLNTHHRALPCFDFDSISRKKRPLDIDPSSAFRRTLPLLL 60

Query: 61  WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR 120
           WNDHPNN DDDP +PNSASSNHP+NPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR
Sbjct: 61  WNDHPNNDDDDPNDPNSASSNHPNNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR 120

Query: 121 PAAENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG 180
           PA ENDGGVYRELN NNSRYVKVKMEGVGIGRKVD+RQHHCLDALRATLMNMFDKPDSDG
Sbjct: 121 PATENDGGVYRELNPNNSRYVKVKMEGVGIGRKVDVRQHHCLDALRATLMNMFDKPDSDG 180

Query: 181 YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKN 216
           YNLTYQDTDGDWLLAEDVTWRDFI+TVQRIKLEKN
Sbjct: 181 YNLTYQDTDGDWLLAEDVTWRDFIQTVQRIKLEKN 215

[Close]

BLAST of Carg09932-RA vs. NCBI nr
Match: XP_022987761.1 (uncharacterized protein LOC111485214 isoform X2 [Cucurbita maxima])

HSP 1 Score: 416.0 bits (1068), Expect = 2.0e-112
Identity = 199/215 (92.56%), Postives = 204/215 (94.88%), Query Frame = 0


Query: 1   MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL 60
           MAMELQL L LFPTNPTKPF LNTHHRALPCLDFDSIS KKRPLDIDPSSAFRRTLPLLL
Sbjct: 79  MAMELQLGLALFPTNPTKPFHLNTHHRALPCLDFDSISGKKRPLDIDPSSAFRRTLPLLL 138

Query: 61  WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR 120
           WNDHPN+GDDDP +PNSASSNHPDNP+EQEIELVGWPPLKKRRKTLFVQKRI RAV IRR
Sbjct: 139 WNDHPNDGDDDPNDPNSASSNHPDNPKEQEIELVGWPPLKKRRKTLFVQKRIRRAVVIRR 198

Query: 121 PAAENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG 180
           PA EN GGV+RELNSNNS YVKVKMEGVGIGRKVDIRQHHC DALRATLMNMFDKPDSDG
Sbjct: 199 PAMENGGGVHRELNSNNSLYVKVKMEGVGIGRKVDIRQHHCFDALRATLMNMFDKPDSDG 258

Query: 181 YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKN 216
           YNLTYQDTDGDWLLA+DVTWRDFIRTVQRIKLEKN
Sbjct: 259 YNLTYQDTDGDWLLADDVTWRDFIRTVQRIKLEKN 293

[Close]

BLAST of Carg09932-RA vs. ExPASy Swiss-Prot
Match: Q93WC4 (Auxin-responsive protein IAA29 OS=Arabidopsis thaliana OX=3702 GN=IAA29 PE=1 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 1.7e-18
Identity = 78/242 (32.23%), Postives = 110/242 (45.45%), Query Frame = 0


Query: 3   MELQLSLGLFPTNPTK---PFDLNTH---HRALPCLDFDSISRKKR-PLDIDPSSAFRRT 62
           MEL L L L P   +K    FDLN H     A  CL  + +  +    L     + +   
Sbjct: 1   MELDLGLSLSPHKSSKLGFNFDLNKHCAIEGAASCLGTEKLRFEATFGLGNVEENCYMPK 60

Query: 63  LPLLLWNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKK-------------R 122
             L   N  PN  D+DP     + S+   + EE+  E+VGWPP+K              R
Sbjct: 61  QRLFALNGQPNEEDEDPL---ESESSIVYDDEEENSEVVGWPPVKTCMIKYGSYHHRHIR 120

Query: 123 RKTLFVQKRIGRAVA-----IRRPAAENDG-GVYRELNSNNSRYVKVKMEGVGIGRKVDI 182
                     GR +      I  P     G      ++S +S YVKVKM+GV I RKVDI
Sbjct: 121 NHHHCPYHHRGRRITAMNNNISNPTTATVGSSSSSSISSRSSMYVKVKMDGVAIARKVDI 180

Query: 183 RQHHCLDALRATLMNMF------DKPDSDGYNLTYQDTDGDWLLAEDVTWRDFIRTVQRI 213
           +  +  ++L  +L+ MF      D+ D++ Y  T+Q  +GDWLL  DVTW+ F  +V RI
Sbjct: 181 KLFNSYESLTNSLITMFTEYEDCDREDTN-YTFTFQGKEGDWLLRGDVTWKIFAESVHRI 238

[Close]

BLAST of Carg09932-RA vs. ExPASy Swiss-Prot
Match: Q38829 (Auxin-responsive protein IAA11 OS=Arabidopsis thaliana OX=3702 GN=IAA11 PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.6e-16
Identity = 51/149 (34.23%), Postives = 75/149 (50.34%), Query Frame = 0


Query: 92  ELVGWPPLKKRRKTLFVQKRIGRAVAIRRPAAENDGGVYRELNSN----NSRYVKVKMEG 151
           ++VGWPP++  R    V +   +A A   P  E    V +  + +    NS +VKV M+G
Sbjct: 88  QVVGWPPIRTYRMNSMVNQ--AKASATEDPNLEISQAVNKNRSDSTKMRNSMFVKVTMDG 147

Query: 152 VGIGRKVDIRQHHCLDALRATLMNMFDKP----------------------DSDGYNLTY 211
           + IGRK+D+  H C ++L  TL  MF KP                       S G  LTY
Sbjct: 148 IPIGRKIDLNAHKCYESLSNTLEEMFLKPKLGSRTLETDGHMETPVKILPDGSSGLVLTY 207

Query: 212 QDTDGDWLLAEDVTWRDFIRTVQRIKLEK 215
           +D +GDW+L  DV W  FI +V+R+++ K
Sbjct: 208 EDKEGDWMLVGDVPWGMFIGSVRRLRIMK 234

[Close]

BLAST of Carg09932-RA vs. ExPASy Swiss-Prot
Match: A2XB18 (Auxin-responsive protein IAA10 OS=Oryza sativa subsp. indica OX=39946 GN=IAA10 PE=2 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 4.7e-16
Identity = 51/162 (31.48%), Postives = 78/162 (48.15%), Query Frame = 0


Query: 74  NPNSASSNHPDNPEEQEIELVGWPPLKK-RRKTLFVQKRIGRAVAIRRPAAENDGGVYRE 133
           +P + +S HP         +VGWPP+++ R  +LF Q +   +    +  A N+  V ++
Sbjct: 97  SPGTVASGHP----HSSFGVVGWPPIRQFRMNSLFNQAKENTSETDTKKTATNESDVQKD 156

Query: 134 LNSNNSR-----YVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKP----------- 193
                 +     +VKV M+G  IGRKVD+  H     L   L  MF KP           
Sbjct: 157 KEEGEKKGRVAGWVKVNMDGEVIGRKVDLNAHRSYKTLALALELMFTKPSIGLCASHNTN 216

Query: 194 ------DSDGYNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKL 213
                 +S  Y LTY+D DGDW+L  DV W  F+ +V+R+++
Sbjct: 217 SLKLLDNSAEYQLTYEDRDGDWMLVGDVPWEMFVSSVKRLRI 254

[Close]

BLAST of Carg09932-RA vs. ExPASy Swiss-Prot
Match: Q0DWF2 (Auxin-responsive protein IAA10 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA10 PE=2 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 4.7e-16
Identity = 51/162 (31.48%), Postives = 78/162 (48.15%), Query Frame = 0


Query: 74  NPNSASSNHPDNPEEQEIELVGWPPLKK-RRKTLFVQKRIGRAVAIRRPAAENDGGVYRE 133
           +P + +S HP         +VGWPP+++ R  +LF Q +   +    +  A N+  V ++
Sbjct: 97  SPGTVASGHP----HSSFGVVGWPPIRQFRMNSLFNQAKENTSETDTKKTATNESDVQKD 156

Query: 134 LNSNNSR-----YVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKP----------- 193
                 +     +VKV M+G  IGRKVD+  H     L   L  MF KP           
Sbjct: 157 KEEGEKKGRVAGWVKVNMDGEVIGRKVDLNAHRSYKTLALALELMFTKPSIGLCASHNTN 216

Query: 194 ------DSDGYNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKL 213
                 +S  Y LTY+D DGDW+L  DV W  F+ +V+R+++
Sbjct: 217 SLKLLDNSAEYQLTYEDRDGDWMLVGDVPWEMFVSSVKRLRI 254

[Close]

BLAST of Carg09932-RA vs. ExPASy Swiss-Prot
Match: Q38830 (Auxin-responsive protein IAA12 OS=Arabidopsis thaliana OX=3702 GN=IAA12 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.5e-14
Identity = 57/180 (31.67%), Postives = 83/180 (46.11%), Query Frame = 0


Query: 58  LLLWNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVA 117
           +L   D P+ G       ++ SS+H      +  ++VGWPP+   R    V  +  +A  
Sbjct: 39  ILTAKDFPSVG----SKRSAESSSHQGASPPRSSQVVGWPPIGLHRMNSLVNNQAMKAAR 98

Query: 118 IRRPAAENDGGVYRELNSNNSR---------YVKVKMEGVGIGRKVDIRQHHCLDALRAT 177
                 E       EL   + +         +VKV M+GVGIGRKVD+R H   + L  T
Sbjct: 99  AEEGDGEKKVVKNDELKDVSMKVNPKVQGLGFVKVNMDGVGIGRKVDMRAHSSYENLAQT 158

Query: 178 LMNMF-----------DKP-----DSDGYNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKL 213
           L  MF            KP      S  + LTY+D +GDW+L  DV WR FI +V+R+++
Sbjct: 159 LEEMFFGMTGTTCREKVKPLRLLDGSSDFVLTYEDKEGDWMLVGDVPWRMFINSVKRLRI 214

[Close]

BLAST of Carg09932-RA vs. ExPASy TrEMBL
Match: A0A6J1HA19 (Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111461467 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 1.9e-121
Identity = 213/215 (99.07%), Postives = 213/215 (99.07%), Query Frame = 0


Query: 1   MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL 60
           MAMELQLSL LFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL
Sbjct: 1   MAMELQLSLALFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL 60

Query: 61  WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR 120
           WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR
Sbjct: 61  WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR 120

Query: 121 PAAENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG 180
           PA ENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG
Sbjct: 121 PAMENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG 180

Query: 181 YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKN 216
           YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKN
Sbjct: 181 YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKN 215

[Close]

BLAST of Carg09932-RA vs. ExPASy TrEMBL
Match: A0A6J1JF88 (Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111485214 PE=3 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 9.6e-113
Identity = 199/215 (92.56%), Postives = 204/215 (94.88%), Query Frame = 0


Query: 1   MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL 60
           MAMELQL L LFPTNPTKPF LNTHHRALPCLDFDSIS KKRPLDIDPSSAFRRTLPLLL
Sbjct: 79  MAMELQLGLALFPTNPTKPFHLNTHHRALPCLDFDSISGKKRPLDIDPSSAFRRTLPLLL 138

Query: 61  WNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIRR 120
           WNDHPN+GDDDP +PNSASSNHPDNP+EQEIELVGWPPLKKRRKTLFVQKRI RAV IRR
Sbjct: 139 WNDHPNDGDDDPNDPNSASSNHPDNPKEQEIELVGWPPLKKRRKTLFVQKRIRRAVVIRR 198

Query: 121 PAAENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSDG 180
           PA EN GGV+RELNSNNS YVKVKMEGVGIGRKVDIRQHHC DALRATLMNMFDKPDSDG
Sbjct: 199 PAMENGGGVHRELNSNNSLYVKVKMEGVGIGRKVDIRQHHCFDALRATLMNMFDKPDSDG 258

Query: 181 YNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKN 216
           YNLTYQDTDGDWLLA+DVTWRDFIRTVQRIKLEKN
Sbjct: 259 YNLTYQDTDGDWLLADDVTWRDFIRTVQRIKLEKN 293

[Close]

BLAST of Carg09932-RA vs. ExPASy TrEMBL
Match: A0A0A0LWC5 (Auxin-responsive protein OS=Cucumis sativus OX=3659 GN=Csa_1G231530 PE=3 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.9e-77
Identity = 146/218 (66.97%), Postives = 173/218 (79.36%), Query Frame = 0


Query: 1   MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLLL 60
           MAMELQL L L PTNP KPFDLNTH+     L FDSI+ KKR   +D  + FR+TLPLLL
Sbjct: 83  MAMELQLGLALIPTNPIKPFDLNTHNNNPISLVFDSINHKKRSRHLDSPATFRQTLPLLL 142

Query: 61  WNDHPNNG-DDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIR 120
           WNDHPN+G DDDP +P+SASSN  D  EE+E  LVGWPPLKKRRK+LF+++ +GR   IR
Sbjct: 143 WNDHPNDGDDDDPNDPHSASSNDSDEEEEEENGLVGWPPLKKRRKSLFMKEGVGRRAVIR 202

Query: 121 RPAAENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDSD 180
           RPA +NDG  +R L+ NNSRYVKVKMEGVGI RKVD+R+HH  DALRATLM MFD+ +S+
Sbjct: 203 RPAVKNDGVFWRGLDLNNSRYVKVKMEGVGIARKVDLREHHSFDALRATLMKMFDETNSE 262

Query: 181 GYNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKNGG 218
           GY LT+Q+T G+WLLAE+VTWR+FI TVQRI LEK  G
Sbjct: 263 GYKLTFQNTKGEWLLAENVTWRNFIGTVQRINLEKKSG 300

[Close]

BLAST of Carg09932-RA vs. ExPASy TrEMBL
Match: A0A1S3B8T8 (Auxin-responsive protein OS=Cucumis melo OX=3656 GN=LOC103487427 PE=3 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 4.7e-75
Identity = 148/220 (67.27%), Postives = 173/220 (78.64%), Query Frame = 0


Query: 1   MAMELQLSLGLFPTNPTKPFDLNTHHRALPCLDFDSISRKKRPLDIDPSSAF-RRTLPLL 60
           MAMELQL L L PTNP KPFDLNTH+     L FDSI+ KKR   +D S  F R TLPLL
Sbjct: 1   MAMELQLGLALIPTNPIKPFDLNTHNNNPISLVFDSINHKKRSRHLDSSPTFPRTTLPLL 60

Query: 61  LWNDHPNNGD-DDPKNPNSASSNHPDNPEEQ-EIELVGWPPLKKRRKTLFVQKRIGRAVA 120
           LWNDHPN+GD DD  +P SASSN  D  EE+ E  LVGWPPLKKRRK+LF+++ +GR   
Sbjct: 61  LWNDHPNDGDGDDSNDPLSASSNDSDEEEEERENGLVGWPPLKKRRKSLFMKEGVGRRAV 120

Query: 121 IRRPAAENDGGVYRELNSNNSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPD 180
           IRRPA +NDGG +R L+ NNSRYVKVKMEGVGI RKVD+R+HH  DALRATLMNMFD+ +
Sbjct: 121 IRRPAVKNDGGFWRGLDLNNSRYVKVKMEGVGIARKVDLREHHSFDALRATLMNMFDETN 180

Query: 181 SDGYNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKNGG 218
           S+GY LT+Q+T+G+WLLAE+VTWR+FI  VQRIKLEK  G
Sbjct: 181 SEGYRLTFQNTNGEWLLAENVTWRNFIGDVQRIKLEKKSG 220

[Close]

BLAST of Carg09932-RA vs. ExPASy TrEMBL
Match: A0A6J1I2H3 (Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111469303 PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 1.1e-68
Identity = 144/219 (65.75%), Postives = 161/219 (73.52%), Query Frame = 0


Query: 3   MELQLSLGLFPTNPTKPFDLNTH---HRALPCLDFDSISRKKRPLDIDPSSAFRRTLPLL 62
           MELQL L L    P KPFDLN     H    CLD  S  RKKRPL +DPSSAFRRTLPLL
Sbjct: 1   MELQLGLSLI---PAKPFDLNNSLLLHDTASCLDLTSTIRKKRPLHVDPSSAFRRTLPLL 60

Query: 63  LWNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVAIR 122
           LWNDHPNNGDDD  +P+SAS++  ++ +E++  LVGWPPLKKR+    +QKR GR     
Sbjct: 61  LWNDHPNNGDDDDNDPHSASNDSDEDEDEEDNRLVGWPPLKKRKTRSLMQKRRGRG---- 120

Query: 123 RPAAENDGGVYRELNSN-NSRYVKVKMEGVGIGRKVDIRQHHCLDALRATLMNMFDKPDS 182
           RPA  N GG    LN N NSRYVKVKMEGVGIGRKVD+ Q+HC D LRATLM MFDK D 
Sbjct: 121 RPAV-NGGG----LNWNSNSRYVKVKMEGVGIGRKVDLSQYHCFDQLRATLMKMFDKMDP 180

Query: 183 DGYNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKLEKNGG 218
           D + LTYQD DGDWLLA+DVTWR+FI TVQRIKLEKN G
Sbjct: 181 DSHRLTYQDIDGDWLLADDVTWRNFIGTVQRIKLEKNRG 207

[Close]

BLAST of Carg09932-RA vs. TAIR 10
Match: AT4G32280.1 (indole-3-acetic acid inducible 29 )

HSP 1 Score: 94.4 bits (233), Expect = 1.2e-19
Identity = 78/242 (32.23%), Postives = 110/242 (45.45%), Query Frame = 0


Query: 3   MELQLSLGLFPTNPTK---PFDLNTH---HRALPCLDFDSISRKKR-PLDIDPSSAFRRT 62
           MEL L L L P   +K    FDLN H     A  CL  + +  +    L     + +   
Sbjct: 1   MELDLGLSLSPHKSSKLGFNFDLNKHCAIEGAASCLGTEKLRFEATFGLGNVEENCYMPK 60

Query: 63  LPLLLWNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKK-------------R 122
             L   N  PN  D+DP     + S+   + EE+  E+VGWPP+K              R
Sbjct: 61  QRLFALNGQPNEEDEDPL---ESESSIVYDDEEENSEVVGWPPVKTCMIKYGSYHHRHIR 120

Query: 123 RKTLFVQKRIGRAVA-----IRRPAAENDG-GVYRELNSNNSRYVKVKMEGVGIGRKVDI 182
                     GR +      I  P     G      ++S +S YVKVKM+GV I RKVDI
Sbjct: 121 NHHHCPYHHRGRRITAMNNNISNPTTATVGSSSSSSISSRSSMYVKVKMDGVAIARKVDI 180

Query: 183 RQHHCLDALRATLMNMF------DKPDSDGYNLTYQDTDGDWLLAEDVTWRDFIRTVQRI 213
           +  +  ++L  +L+ MF      D+ D++ Y  T+Q  +GDWLL  DVTW+ F  +V RI
Sbjct: 181 KLFNSYESLTNSLITMFTEYEDCDREDTN-YTFTFQGKEGDWLLRGDVTWKIFAESVHRI 238

[Close]

BLAST of Carg09932-RA vs. TAIR 10
Match: AT4G28640.1 (indole-3-acetic acid inducible 11 )

HSP 1 Score: 87.8 bits (216), Expect = 1.2e-17
Identity = 51/149 (34.23%), Postives = 75/149 (50.34%), Query Frame = 0


Query: 92  ELVGWPPLKKRRKTLFVQKRIGRAVAIRRPAAENDGGVYRELNSN----NSRYVKVKMEG 151
           ++VGWPP++  R    V +   +A A   P  E    V +  + +    NS +VKV M+G
Sbjct: 88  QVVGWPPIRTYRMNSMVNQ--AKASATEDPNLEISQAVNKNRSDSTKMRNSMFVKVTMDG 147

Query: 152 VGIGRKVDIRQHHCLDALRATLMNMFDKP----------------------DSDGYNLTY 211
           + IGRK+D+  H C ++L  TL  MF KP                       S G  LTY
Sbjct: 148 IPIGRKIDLNAHKCYESLSNTLEEMFLKPKLGSRTLETDGHMETPVKILPDGSSGLVLTY 207

Query: 212 QDTDGDWLLAEDVTWRDFIRTVQRIKLEK 215
           +D +GDW+L  DV W  FI +V+R+++ K
Sbjct: 208 EDKEGDWMLVGDVPWGMFIGSVRRLRIMK 234

[Close]

BLAST of Carg09932-RA vs. TAIR 10
Match: AT4G28640.2 (indole-3-acetic acid inducible 11 )

HSP 1 Score: 87.8 bits (216), Expect = 1.2e-17
Identity = 51/149 (34.23%), Postives = 75/149 (50.34%), Query Frame = 0


Query: 92  ELVGWPPLKKRRKTLFVQKRIGRAVAIRRPAAENDGGVYRELNSN----NSRYVKVKMEG 151
           ++VGWPP++  R    V +   +A A   P  E    V +  + +    NS +VKV M+G
Sbjct: 88  QVVGWPPIRTYRMNSMVNQ--AKASATEDPNLEISQAVNKNRSDSTKMRNSMFVKVTMDG 147

Query: 152 VGIGRKVDIRQHHCLDALRATLMNMFDKP----------------------DSDGYNLTY 211
           + IGRK+D+  H C ++L  TL  MF KP                       S G  LTY
Sbjct: 148 IPIGRKIDLNAHKCYESLSNTLEEMFLKPKLGSRTLETDGHMETPVKILPDGSSGLVLTY 207

Query: 212 QDTDGDWLLAEDVTWRDFIRTVQRIKLEK 215
           +D +GDW+L  DV W  FI +V+R+++ K
Sbjct: 208 EDKEGDWMLVGDVPWGMFIGSVRRLRIMK 234

[Close]

BLAST of Carg09932-RA vs. TAIR 10
Match: AT4G28640.3 (indole-3-acetic acid inducible 11 )

HSP 1 Score: 87.8 bits (216), Expect = 1.2e-17
Identity = 51/149 (34.23%), Postives = 75/149 (50.34%), Query Frame = 0


Query: 92  ELVGWPPLKKRRKTLFVQKRIGRAVAIRRPAAENDGGVYRELNSN----NSRYVKVKMEG 151
           ++VGWPP++  R    V +   +A A   P  E    V +  + +    NS +VKV M+G
Sbjct: 88  QVVGWPPIRTYRMNSMVNQ--AKASATEDPNLEISQAVNKNRSDSTKMRNSMFVKVTMDG 147

Query: 152 VGIGRKVDIRQHHCLDALRATLMNMFDKP----------------------DSDGYNLTY 211
           + IGRK+D+  H C ++L  TL  MF KP                       S G  LTY
Sbjct: 148 IPIGRKIDLNAHKCYESLSNTLEEMFLKPKLGSRTLETDGHMETPVKILPDGSSGLVLTY 207

Query: 212 QDTDGDWLLAEDVTWRDFIRTVQRIKLEK 215
           +D +GDW+L  DV W  FI +V+R+++ K
Sbjct: 208 EDKEGDWMLVGDVPWGMFIGSVRRLRIMK 234

[Close]

BLAST of Carg09932-RA vs. TAIR 10
Match: AT1G04550.2 (AUX/IAA transcriptional regulator family protein )

HSP 1 Score: 81.3 bits (199), Expect = 1.1e-15
Identity = 57/180 (31.67%), Postives = 83/180 (46.11%), Query Frame = 0


Query: 58  LLLWNDHPNNGDDDPKNPNSASSNHPDNPEEQEIELVGWPPLKKRRKTLFVQKRIGRAVA 117
           +L   D P+ G       ++ SS+H      +  ++VGWPP+   R    V  +  +A  
Sbjct: 39  ILTAKDFPSVG----SKRSAESSSHQGASPPRSSQVVGWPPIGLHRMNSLVNNQAMKAAR 98

Query: 118 IRRPAAENDGGVYRELNSNNSR---------YVKVKMEGVGIGRKVDIRQHHCLDALRAT 177
                 E       EL   + +         +VKV M+GVGIGRKVD+R H   + L  T
Sbjct: 99  AEEGDGEKKVVKNDELKDVSMKVNPKVQGLGFVKVNMDGVGIGRKVDMRAHSSYENLAQT 158

Query: 178 LMNMF-----------DKP-----DSDGYNLTYQDTDGDWLLAEDVTWRDFIRTVQRIKL 213
           L  MF            KP      S  + LTY+D +GDW+L  DV WR FI +V+R+++
Sbjct: 159 LEEMFFGMTGTTCREKVKPLRLLDGSSDFVLTYEDKEGDWMLVGDVPWRMFINSVKRLRI 214

The following BLAST results are available for this feature:

BLAST of Carg09932-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023504.1	8.6e-124	100.00	Auxin-responsive protein IAA29, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]
KAG6589834.1	5.6e-123	99.08	Auxin-responsive protein IAA29, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022960765.1	4.0e-121	99.07	uncharacterized protein LOC111461467 isoform X2 [Cucurbita moschata]	[more]
XP_023515749.1	4.6e-117	95.35	uncharacterized protein LOC111779818 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022987761.1	2.0e-112	92.56	uncharacterized protein LOC111485214 isoform X2 [Cucurbita maxima]	[more]

BLAST of Carg09932-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93WC4	1.7e-18	32.23	Auxin-responsive protein IAA29 OS=Arabidopsis thaliana OX=3702 GN=IAA29 PE=1 SV=...	[more]
Q38829	1.6e-16	34.23	Auxin-responsive protein IAA11 OS=Arabidopsis thaliana OX=3702 GN=IAA11 PE=1 SV=...	[more]
A2XB18	4.7e-16	31.48	Auxin-responsive protein IAA10 OS=Oryza sativa subsp. indica OX=39946 GN=IAA10 P...	[more]
Q0DWF2	4.7e-16	31.48	Auxin-responsive protein IAA10 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA10...	[more]
Q38830	1.5e-14	31.67	Auxin-responsive protein IAA12 OS=Arabidopsis thaliana OX=3702 GN=IAA12 PE=1 SV=...	[more]

BLAST of Carg09932-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HA19	1.9e-121	99.07	Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111461467 PE=3 SV=1	[more]
A0A6J1JF88	9.6e-113	92.56	Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111485214 PE=3 SV=1	[more]
A0A0A0LWC5	2.9e-77	66.97	Auxin-responsive protein OS=Cucumis sativus OX=3659 GN=Csa_1G231530 PE=3 SV=1	[more]
A0A1S3B8T8	4.7e-75	67.27	Auxin-responsive protein OS=Cucumis melo OX=3656 GN=LOC103487427 PE=3 SV=1	[more]
A0A6J1I2H3	1.1e-68	65.75	Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111469303 PE=3 SV=1	[more]

BLAST of Carg09932-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32280.1	1.2e-19	32.23	indole-3-acetic acid inducible 29	[more]
AT4G28640.1	1.2e-17	34.23	indole-3-acetic acid inducible 11	[more]
AT4G28640.2	1.2e-17	34.23	indole-3-acetic acid inducible 11	[more]
AT4G28640.3	1.2e-17	34.23	indole-3-acetic acid inducible 11	[more]
AT1G04550.2	1.1e-15	31.67	AUX/IAA transcriptional regulator family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR033389	AUX/IAA domain	PFAM	PF02309	AUX_IAA	coord: 179..214 e-value: 2.6E-8 score: 34.1 coord: 72..175 e-value: 2.2E-16 score: 60.5
None	No IPR available	GENE3D	3.10.20.90		coord: 133..216 e-value: 4.0E-22 score: 80.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 62..95
None	No IPR available	PANTHER	PTHR31734:SF38	AUXIN-RESPONSIVE PROTEIN IAA20	coord: 3..215
None	No IPR available	SUPERFAMILY	54277	CAD & PB1 domains	coord: 132..212
IPR003311	AUX/IAA protein	PANTHER	PTHR31734	AUXIN-RESPONSIVE PROTEIN IAA17	coord: 3..215
IPR000270	PB1 domain	PROSITE	PS51745	PB1	coord: 138..217 score: 21.290037

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg09932	Carg09932	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg09932-RA:1	Carg09932-RA:1	exon
Carg09932-RA:2	Carg09932-RA:2	exon
Carg09932-RA:3	Carg09932-RA:3	exon
Carg09932-RA:4	Carg09932-RA:4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg09932-RA:cds	Carg09932-RA:cds	CDS
Carg09932-RA:cds	Carg09932-RA:cds_2	CDS
Carg09932-RA:cds	Carg09932-RA:cds_3	CDS
Carg09932-RA:cds	Carg09932-RA:cds_4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg09932-RA:three_prime_utr	Carg09932-RA:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg09932-RA	Carg09932-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0009734	auxin-activated signaling pathway
biological_process	GO:0006487	protein N-linked glycosylation
cellular_component	GO:0005634	nucleus
cellular_component	GO:0016020	membrane
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003830	beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity