Carg01243-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01243-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Arabinogalactan peptide 23
Location	Carg_Chr06: 440374 .. 440660 (+)
Sequence length	204
RNA-Seq Expression	Carg01243-RA
Synteny	Carg01243-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATGAAGAAGGTTACCTGCGCCCTCCTCTTCGCTGCTGCCACCGTGAGCGCGGTTGTGGCCTCCGCTGAAGCCCCAGCTCTGGCCCCTGGCCCCTCCTCCGATGCGGCTGCTGCTGCTGTGCCTGCTGTTGGAGCGTTCATTGGCGCTTCTGTTGCGACGTTGTTTGCGTACTACTTGTAAGAAGGTGAGTGAGGGAATGAGGAGAGAATAGGAGGGCTCTTGGGGAGCGATGAAGGGAGATTATGTAACATGGGAACAGAGAAACTTGTAAAGGGTTTTGA

mRNA sequence

ATGGCCATGAAGAAGGTTACCTGCGCCCTCCTCTTCGCTGCTGCCACCGTGAGCGCGGTTGTGGCCTCCGCTGAAGCCCCAGCTCTGGCCCCTGGCCCCTCCTCCGATGCGGCTGCTGCTGCTGTGCCTGCTGTTGGAGCGTTCATTGGCGCTTCTGTTGCGACGTTGTTTGCGTACTACTTAGAAACTTGTAAAGGGTTTTGA

Coding sequence (CDS)

ATGGCCATGAAGAAGGTTACCTGCGCCCTCCTCTTCGCTGCTGCCACCGTGAGCGCGGTTGTGGCCTCCGCTGAAGCCCCAGCTCTGGCCCCTGGCCCCTCCTCCGATGCGGCTGCTGCTGCTGTGCCTGCTGTTGGAGCGTTCATTGGCGCTTCTGTTGCGACGTTGTTTGCGTACTACTTAGAAACTTGTAAAGGGTTTTGA

Protein sequence

MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYYLETCKGF

Homology

[Close]

BLAST of Carg01243-RA vs. NCBI nr
Match: KAG7027762.1 (hypothetical protein SDJN02_08939, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 113.6 bits (283), Expect = 6.5e-22
Identity = 67/67 (100.00%), Postives = 67/67 (100.00%), Query Frame = 0


Query: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY 60
          MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY
Sbjct: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY 60

Query: 61 LETCKGF 68
          LETCKGF
Sbjct: 61 LETCKGF 67

[Close]

BLAST of Carg01243-RA vs. NCBI nr
Match: KAG6596222.1 (putative pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 90.5 bits (223), Expect = 5.9e-15
Identity = 55/61 (90.16%), Postives = 59/61 (96.72%), Query Frame = 0


Query: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY 60
          MA+ K+TCALLFAAATV+AVVAS EAPALAPGPSSDAAAAAVPAVGAFIGASVA+LFAYY
Sbjct: 1  MAINKLTCALLFAAATVTAVVASTEAPALAPGPSSDAAAAAVPAVGAFIGASVASLFAYY 60

Query: 61 L 62
          L
Sbjct: 61 L 61

[Close]

BLAST of Carg01243-RA vs. NCBI nr
Match: KAG7027763.1 (putative pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 79.0 bits (193), Expect = 1.8e-11
Identity = 49/58 (84.48%), Postives = 54/58 (93.10%), Query Frame = 0


Query: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFA 59
          MA+ K+TCALLFAAATV+AVVAS EAPALAPGPSSDAAAAAVPAVGAFIGASVA+  +
Sbjct: 1  MAINKLTCALLFAAATVTAVVASTEAPALAPGPSSDAAAAAVPAVGAFIGASVASFLS 58

[Close]

BLAST of Carg01243-RA vs. NCBI nr
Match: XP_038906349.1 (arabinogalactan protein 23 [Benincasa hispida])

HSP 1 Score: 71.2 bits (173), Expect = 3.7e-09
Identity = 45/61 (73.77%), Postives = 52/61 (85.25%), Query Frame = 0


Query: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY 60
          M MKKVTCA+L +AA +SA +AS E PA APG SSDAAAAA+PAVGAFIGAS+A+ FAYY
Sbjct: 1  MDMKKVTCAVLVSAAVMSATMASGEGPAPAPGLSSDAAAAALPAVGAFIGASLASFFAYY 60

Query: 61 L 62
          L
Sbjct: 61 L 61

[Close]

BLAST of Carg01243-RA vs. NCBI nr
Match: KAA0043704.1 (Arabinogalactan peptide 23 [Cucumis melo var. makuwa] >TYK25430.1 Arabinogalactan peptide 23 [Cucumis melo var. makuwa])

HSP 1 Score: 62.0 bits (149), Expect = 2.3e-06
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 0


Query: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY 60
          M MKKVTCA+L AAA VSA +A++E PA AP  SSD A AA+PAVGAFIGAS+ + FAYY
Sbjct: 1  MDMKKVTCAVLVAAAAVSATMATSEGPAPAPSLSSD-ATAALPAVGAFIGASLVSFFAYY 60

Query: 61 L 62
          L
Sbjct: 61 L 60

[Close]

BLAST of Carg01243-RA vs. ExPASy Swiss-Prot
Match: Q8S2W4 (Arabinogalactan protein 23 OS=Arabidopsis thaliana OX=3702 GN=AGP23 PE=1 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 2.1e-07
Identity = 35/61 (57.38%), Postives = 51/61 (83.61%), Query Frame = 0


Query: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY 60
          M MKK+ C +LFAAA+++AV+A+ E  A APGP+S AA+AA+PA+G+ +GAS+ +LF+YY
Sbjct: 1  MEMKKIACGVLFAAASMTAVMAAEEVGAPAPGPAS-AASAALPALGSLVGASLVSLFSYY 60

Query: 61 L 62
          L
Sbjct: 61 L 60

[Close]

BLAST of Carg01243-RA vs. ExPASy TrEMBL
Match: A0A5D3DQ83 (Arabinogalactan peptide 23 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005730 PE=4 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.1e-06
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 0


Query: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY 60
          M MKKVTCA+L AAA VSA +A++E PA AP  SSD A AA+PAVGAFIGAS+ + FAYY
Sbjct: 1  MDMKKVTCAVLVAAAAVSATMATSEGPAPAPSLSSD-ATAALPAVGAFIGASLVSFFAYY 60

Query: 61 L 62
          L
Sbjct: 61 L 60

[Close]

BLAST of Carg01243-RA vs. ExPASy TrEMBL
Match: A0A4P1RCA0 (Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_20074 PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.4e-06
Identity = 40/61 (65.57%), Postives = 48/61 (78.69%), Query Frame = 0


Query: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY 60
          M MKKV CA+LFAAA++SAV A+AEAPA APGPSS AA      VG+F+GASV ++ AYY
Sbjct: 1  MEMKKVACAVLFAAASISAVAAAAEAPAPAPGPSSGAA-----TVGSFVGASVLSIVAYY 56

Query: 61 L 62
          L
Sbjct: 61 L 56

[Close]

BLAST of Carg01243-RA vs. ExPASy TrEMBL
Match: A0A6P5Z0H7 (arabinogalactan peptide 23-like OS=Durio zibethinus OX=66656 GN=LOC111296195 PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.4e-06
Identity = 34/62 (54.84%), Postives = 52/62 (83.87%), Query Frame = 0


Query: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY 60
          M MKK++CA++ AAA++SAV+AS +APA APGP+S AA+A +P +G+ +GAS+ + FAY+
Sbjct: 1  MDMKKISCAVIVAAASMSAVMASTDAPAAAPGPASSAASATLPVLGSLVGASLVSFFAYH 60

Query: 61 LE 63
          L+
Sbjct: 61 LQ 62

[Close]

BLAST of Carg01243-RA vs. ExPASy TrEMBL
Match: A0A6P6APJ2 (arabinogalactan peptide 23-like OS=Durio zibethinus OX=66656 GN=LOC111311539 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 3.2e-06
Identity = 33/62 (53.23%), Postives = 51/62 (82.26%), Query Frame = 0


Query: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY 60
          M MKK++CA++  AA++SAV+AS +APA APGP+S AA++ +P +G+ +GAS+ + FAYY
Sbjct: 1  MDMKKISCAVIVVAASMSAVMASTDAPAAAPGPASSAASSPLPVLGSLVGASLVSFFAYY 60

Query: 61 LE 63
          L+
Sbjct: 61 LQ 62

[Close]

BLAST of Carg01243-RA vs. ExPASy TrEMBL
Match: A0A6J1J6Y9 (arabinogalactan peptide 23-like OS=Cucurbita maxima OX=3661 GN=LOC111481924 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 5.4e-06
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 0


Query: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY 60
          M MKK+ CA+LFAAATVSAVVA  EA A APGPSS  AAA +PA+G+ IGAS+ ++ AYY
Sbjct: 1  MEMKKIACAVLFAAATVSAVVAHDEALAPAPGPSS-GAAAGLPAIGSAIGASLLSVAAYY 60

Query: 61 LE 63
          L+
Sbjct: 61 LQ 61

[Close]

BLAST of Carg01243-RA vs. TAIR 10
Match: AT3G57690.1 (arabinogalactan protein 23 )

HSP 1 Score: 55.8 bits (133), Expect = 1.5e-08
Identity = 35/61 (57.38%), Postives = 51/61 (83.61%), Query Frame = 0


Query: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY 60
          M MKK+ C +LFAAA+++AV+A+ E  A APGP+S AA+AA+PA+G+ +GAS+ +LF+YY
Sbjct: 1  MEMKKIACGVLFAAASMTAVMAAEEVGAPAPGPAS-AASAALPALGSLVGASLVSLFSYY 60

Query: 61 L 62
          L
Sbjct: 61 L 60

[Close]

BLAST of Carg01243-RA vs. TAIR 10
Match: AT2G41905.1 (BEST Arabidopsis thaliana protein match is: arabinogalactan protein 23 (TAIR:AT3G57690.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 54.7 bits (130), Expect = 3.4e-08
Identity = 34/61 (55.74%), Postives = 49/61 (80.33%), Query Frame = 0


Query: 1  MAMKKVTCALLFAAATVSAVVASAEAPALAPGPSSDAAAAAVPAVGAFIGASVATLFAYY 60
          M MKK+ C ++FAAA+++AV+A AE  A APGP++  A+ AVPA+G+ +GAS+ +LFAYY
Sbjct: 1  MEMKKIACGVVFAAASMTAVMA-AEVGAPAPGPAASGASVAVPALGSLVGASLVSLFAYY 60

Query: 61 L 62
          L
Sbjct: 61 L 60

The following BLAST results are available for this feature:

BLAST of Carg01243-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027762.1	6.5e-22	100.00	hypothetical protein SDJN02_08939, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6596222.1	5.9e-15	90.16	putative pentatricopeptide repeat-containing protein, mitochondrial, partial [Cu...	[more]
KAG7027763.1	1.8e-11	84.48	putative pentatricopeptide repeat-containing protein, mitochondrial, partial [Cu...	[more]
XP_038906349.1	3.7e-09	73.77	arabinogalactan protein 23 [Benincasa hispida]	[more]
KAA0043704.1	2.3e-06	68.85	Arabinogalactan peptide 23 [Cucumis melo var. makuwa] >TYK25430.1 Arabinogalacta...	[more]

BLAST of Carg01243-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S2W4	2.1e-07	57.38	Arabinogalactan protein 23 OS=Arabidopsis thaliana OX=3702 GN=AGP23 PE=1 SV=2	[more]

BLAST of Carg01243-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DQ83	1.1e-06	68.85	Arabinogalactan peptide 23 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A4P1RCA0	1.4e-06	65.57	Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_20074 PE=4 S...	[more]
A0A6P5Z0H7	1.4e-06	54.84	arabinogalactan peptide 23-like OS=Durio zibethinus OX=66656 GN=LOC111296195 PE=...	[more]
A0A6P6APJ2	3.2e-06	53.23	arabinogalactan peptide 23-like OS=Durio zibethinus OX=66656 GN=LOC111311539 PE=...	[more]
A0A6J1J6Y9	5.4e-06	66.13	arabinogalactan peptide 23-like OS=Cucurbita maxima OX=3661 GN=LOC111481924 PE=4...	[more]

BLAST of Carg01243-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57690.1	1.5e-08	57.38	arabinogalactan protein 23	[more]
AT2G41905.1	3.4e-08	55.74	BEST Arabidopsis thaliana protein match is: arabinogalactan protein 23 (TAIR:AT3...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044702	Arabinogalactan protein 23/40	PANTHER	PTHR34672	POLLEN-SPECIFIC ARABINOGALACTA PROTEIN BAN102	coord: 1..61
None	No IPR available	PANTHER	PTHR34672:SF2	ARABINOGALACTAN PROTEIN 23	coord: 1..61

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg01243	Carg01243	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg01243-RA:2	Carg01243-RA:2	exon
Carg01243-RA:1	Carg01243-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg01243-RA:cds	Carg01243-RA:cds	CDS
Carg01243-RA:cds	Carg01243-RA:cds_2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg01243-RA	Carg01243-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane