CSPI07G10400.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI07G10400.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Reverse transcriptase domain-containing protein
Location	Chr7: 8501993 .. 8506164 (+)
Sequence length	1251
RNA-Seq Expression	CSPI07G10400.1
Synteny	CSPI07G10400.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTAAGAAGTTTGACCACAAGTGGATTGAATGGATCTTGGGTTGTGTTAAGAACCTGAGATACTCAATATTCCTCAATAGAAGACCGGGAGGAAGAACGTTGGCTACAAAGGTTATCAGGCAGGGTGACCCCCTTTCGTCGTTCCTCTTCATACTTGTAAGTGAAGTTCTTGATGCTCTAATTGAAAATCTACATCAAAATGGCCTTTATGAAGGTTTTGTAGTGGGAAAGGACAAGATTCATGTTCCAATCCTTCAATTTGCAGATTACACCCTACTTTTTGCAAATGCGAAAGCAACATGTTAGTAACTTTGAAACAGACAATAGAGCTTTTTGAATGGTGCTCCTGGCAAAAAGTGAACTGGGACAAGTCGGCCCTTTTTAGATAAACGTGGAAGAGATATCCTCTTTTTGGCAACCAGTTCTTGATAAAATTCAACTAAAGTTAGATGAATGGAAGAGGTTTAACCTCTCGAGAGGGGGGGAGCTATGCTTTGTAAGTCTATATTCCTAATGCCAGAAGAAGTCACTTCAAAATTGGAAAGAATCCTGAGGAATTTCTTTTGGAAGGGAGGGCAGCAAATTAAACTACCTTGTTAGTTAGAAAGTGGTCAACAAATTCTTGATGGATGGTGGCCCTTGGCCTTGGTGGCTTCAAATCTAAAAACTTGGCGCTTCTAGCCAAATGGGAGTGGAGATACATGGAAGAAGACTCTCCGTGGTGCAAGGTTATCAGGAGTATCCATGACAAAGATTCCTTTAATCGGCATACTGCCAGAAAAGAAGTCAAATGCGTGAGAAGCCCTTGGGTTATTATTTCGAGAACTTGGTTGAAAATGGAATCTTTAGCCACCTTTAGCTTGGGAATGGGAGAAGTATAGCTTTTTGGTTAGATCACTTGGGGGAGTGACCATTTCATCTAGCCTTTCCACAGTTGTTCAGAATAGCTTTACTCCCAAATGTTTCGGTTGCTGATCGCTGAGACTCTGCATTATCATCTTGGTCCCTAATCTTCTGCAGTTTGCTAAAAGATGAAGAGATTTCAGACTTCCAAGTACTGCTTCGAAAATTAGCATCAAGAAATATTTCTGAAGTTCAAGACAGACAAATATGGTCACCTGGCGGCAGCAGAAAATTTACAGTAAAGTCCCTTACTCTTCACCTGTCTGCTTCCTCCCCTCTGGATAAATTCCTCTATACGGCCCTTTGGAAGTCTAATAGCCCAAGAAGGGTTAATATCTTCATTTGGATCACCCTGATAGGTTAACTAAATGTGTCCTCAGCTTTGCAACATAAATTGGCTAATAGTTGCCTCTCTCCCTCCATTTGCCCGCTGTGTCTACAACATGAAGAAGATTGCCAACACTTGTTCTTCCTATGCAGCTTTTCGGCCAATTGTTGGGAAAGTCTCCTCAACTTCTTTAATATTGGGCGTTTGGTGCAACTTTTTGTGATAATGTGATTCCTCTGTTAATCGGCTCCTGTCTAAATAGAGAAACTTCTTTACTTTGGGTTAACACGGTGAAAGCTATACTGGCAGAAATCTGGTTTGAGAGGAATATAGGGTGTTCCATGATAAATCTCTTGATTGGAAGGATCACTTTAGAAACGCGCATCAAAATACCTCCTCATGGTGTTCGGCCTCAAAGATCTTCAGCAGCTACAGCATCCAAGGGCTCAACTTAAATTGGAGTGCCTTCATTTTTCTCCAGCTTGATTATTCCCCAATGTGTTTGCGCTTCTGTTTTTTTCTTTTTTTGGTCGTACGCAATCTTTATAGCTTTCCTCCTTTGTTTTGGAATCTCTTTCTTCTCCTACTAGTTATCTTGTAAGCACTTGTTTAACTCTTTTGTACATTGAACTTTTTGCCTCATCACGAATATTAACAAAGCTACTCGTTTCCATTTAAAAAAAGGTGTACAGTTTTATCTCTTTGCAAATGGTACAATTTTCAAGGAAACAATTTTTTTCTTTTGGGGGGGGGGTAAGAAATCAAGTTTTCTTTGATAAAAGTTTATACAGGGACATACATTCGTTCTCAAAAAGTCCAACTATCTACAAGAATGGGCTGCGGTCCCTCAAACTTACAAGAAATCCGTTCTTACCCGAGGAAACAAATCTTTTGTTAACCTTTCTGTCACATCTCTTAGTTAGCCAGTGCACACAATTTCTCAATTATAGTTCTTGGTTTGAAGCTCAGCTCCTTCCAAATTACAATTTTTTTTATTTACTTTATTATTGATTATCATTTTTTTTTCCATTTGCAGGAGTTATTGGCCATTCAACAACAAGGTCCTAGAGCCATTGGCTTCTTTGGAACCCGAAATATGGGTTTCTTGCATCAAGAACTCATTGAGATTCTTAGCTATGCAATGGTTATAACGGTAGAATATGTGATTTATTGTCGCTGCACTATTGAAACGCTATTTAGTCTTTCATGACTGCAAAATATTCATATTGCTTAGTATATTCTGTATGCAGAAGAACCACATCTATACTTCAGGAGCATCTGGAACCAATGCAGCAGTTATCAGAGGTGCATTGAGGGCTGAAAAACCAGAACTACTTACTGTCATTTTGCCACAAAGTTTGAAAAAACAACCTCCCGAAAGCCAGGAATTGTTATCCAAAGTAAGTTTGATTGGTTATAAACATATTTACTCAAGAGTTAGAGCTTGTATATGTGTACTGTTTGATTGGTTTAGTCAAGTTTCCGTTTTGAGTTATTTAAAATGGCTTAGGGGGGCATTTGTTGCACAAAGTTGAGTTAGTAATTAAATTATTTAGAGTGTAGAGTTAAGTTGAGTTAGAGTATCTGGTGTAATATTTCTAAAGGATTCTGATTTTTGTTTTTTTCTAAATTTTTTTGTGCAAGTATTAAATTGCTGCATATTCTTCTAAAACTTTCAGTACTCAAATTACATTAACATAATCTTTTCCATTCTTAAAAAGTTATTTTGAACACTCATTTCCTTGACATATTTTTGCTCAATTCTTAAAAGCTTGAGATATCCTAAACACTAATTTTGGACACCTAATTACTTAAAAACTTAGCATATCTTTTTTCCATTCTTAAAAACTCAACATATTCTTGAAAACTTAGCATATTCTGAACACAAGTTTTCATTGCTCAATTACTTTAAGACGTGGTATGTTATTGTATTTCATTTTCAAATGAAATTTAATGCAAAATTAAGTCACAATACACCAAAATTGATTCACACTCAAATACTTAAAAATTTCATTCATACTCGAAGAGAGAAATTTGTGGTACATTTCTTTTTTCTATTTTAGGGCTATTTAGGGTTTTTTTTTGCCATGATACTGAAATTATGATTTTTAAAACGTTTTTTTTTACTTCAAAACAATTTAGCTGATTTCAAAACAATTTTATTTATTTTCAAAAAGTTTTTAGTTCAAAACAGTTCTGTTTACTTTTATGTCAAATTACTAGTTTTGATATAGTGCAAACATTTTCTTAAAAATCCTTCCATTTCAAACAGCACGATAGTACAGTAAGTGTACTGTTAGGAGAGGCTCTCAACTGTTGCAAAATCACTGGTCTGACTCGTAATAGAGAATGAATTTAATAGAACAGTGTTTGTTTTTATTCTGTTTTGTGTGTGTGCTGTTCTGGTGTTTTCCAGGTGCTACTAAGTTGGATTTTTAACAAAAAACTTCGGAAATCAAATGTAGTTTCGTTGCACTTGAAAATATCACAAGTTATTTTGTTAAGAGTTTTTACAGTGTGCTTTTAGAGTCAGAAAATAGTTTAAACACCATTTCTAAATCTTCCCCAACTTTTGATTGTCTATATCAACATTCTCTGTTAATATTATAGAGAATGAATTGTAAGGGCCCTTCAGTCAACTTTTTTTTTTTTTTTAAATTGTTCCTTTGGTCAACTGCTTAGATGATCTATTGTTGATGACTTCATTTGGATAAGTCTTTATTAAATTTTTTTTCTATTTTGTTTATCTTGGAATTTGTAGGTTAGGAATGTGATAGAGAAGCCCCACAATGATCATTTATCTTTGATAGAAGCTAGCAGGTATGTGTGTAGTTTTTTTCTTAGTTGACCATAAGGTGAATTAGAATTTCACATAGTGAAAATATTTTTACTGATGTTGATTTTTAATAATAGTTTATTGGTAAAGAAAAAGAATTAG

mRNA sequence

ATGAGTAAGAAGTTTGACCACAAGTGGATTGAATGGATCTTGGGTTGTGTTAAGAACCTGAGATACTCAATATTCCTCAATAGAAGACCGGGAGGAAGAACGTTGGCTACAAAGGTTATCAGGCAGGGTGACCCCCTTTCGTCGTTCCTCTTCATACTTGTAAGTGAAGTTCTTGATGCTCTAATTGAAAATCTACATCAAAATGGCCTTTATGAAGGTTTTGTAGTGGGAAAGGACAAGATTCATGTTCCAATCCTTCAATTTGCAGATTACACCCTACTTTTTGCAAATGCGAAAGCAACATTTCTTGATAAAATTCAACTAAAGTTAGATGAATGGAAGAGGTTTAACCTCTCGAGAGGGGGGGAGCTATGCTTTCCAAATGGGAGTGGAGATACATGGAAGAAGACTCTCCGTGGTGCAAGGTTATCAGGAGTATCCATGACAAAGATTCCTTTAATCGGCATACTGCCAGAAAAGAAGTCAAATGCTTTGCTAAAAGATGAAGAGATTTCAGACTTCCAAGTACTGCTTCGAAAATTAGCATCAAGAAATATTTCTGAAGTTCAAGACAGACAAATATGGTCACCTGGCGGCAGCAGAAAATTTACAGTAAAGTCCCTTACTCTTCACCTGTCTGCTTCCTCCCCTCTGGATAAATTCCTCTATACGGCCCTTTGGAAGTCTAATAGCCCAAGAAGGCTTTGCAACATAAATTGGCTAATAGTTGCCTCTCTCCCTCCATTTGCCCGCTGTGTCTACAACATGAAGAAGATTGCCAACACTTGTTCTTCCTATGCAGCTTTTCGGCCAATTGTTGGGAAAGGACATACATTCGTTCTCAAAAAGTCCAACTATCTACAAGAATGGGCTGCGGTCCCTCAAACTTACAAGAAATCCGAGTTATTGGCCATTCAACAACAAGGTCCTAGAGCCATTGGCTTCTTTGGAACCCGAAATATGGGTTTCTTGCATCAAGAACTCATTGAGATTCTTAGCTATGCAATGGTTATAACGAAGAACCACATCTATACTTCAGGAGCATCTGGAACCAATGCAGCAGTTATCAGAGGTGCATTGAGGGCTGAAAAACCAGAACTACTTACTGTCATTTTGCCACAAAGTTTGAAAAAACAACCTCCCGAAAGCCAGGAATTGTTATCCAAAGTTAGGAATGTGATAGAGAAGCCCCACAATGATCATTTATCTTTGATAGAAGCTAGCAGTTTATTGGTAAAGAAAAAGAATTAG

Coding sequence (CDS)

ATGAGTAAGAAGTTTGACCACAAGTGGATTGAATGGATCTTGGGTTGTGTTAAGAACCTGAGATACTCAATATTCCTCAATAGAAGACCGGGAGGAAGAACGTTGGCTACAAAGGTTATCAGGCAGGGTGACCCCCTTTCGTCGTTCCTCTTCATACTTGTAAGTGAAGTTCTTGATGCTCTAATTGAAAATCTACATCAAAATGGCCTTTATGAAGGTTTTGTAGTGGGAAAGGACAAGATTCATGTTCCAATCCTTCAATTTGCAGATTACACCCTACTTTTTGCAAATGCGAAAGCAACATTTCTTGATAAAATTCAACTAAAGTTAGATGAATGGAAGAGGTTTAACCTCTCGAGAGGGGGGGAGCTATGCTTTCCAAATGGGAGTGGAGATACATGGAAGAAGACTCTCCGTGGTGCAAGGTTATCAGGAGTATCCATGACAAAGATTCCTTTAATCGGCATACTGCCAGAAAAGAAGTCAAATGCTTTGCTAAAAGATGAAGAGATTTCAGACTTCCAAGTACTGCTTCGAAAATTAGCATCAAGAAATATTTCTGAAGTTCAAGACAGACAAATATGGTCACCTGGCGGCAGCAGAAAATTTACAGTAAAGTCCCTTACTCTTCACCTGTCTGCTTCCTCCCCTCTGGATAAATTCCTCTATACGGCCCTTTGGAAGTCTAATAGCCCAAGAAGGCTTTGCAACATAAATTGGCTAATAGTTGCCTCTCTCCCTCCATTTGCCCGCTGTGTCTACAACATGAAGAAGATTGCCAACACTTGTTCTTCCTATGCAGCTTTTCGGCCAATTGTTGGGAAAGGACATACATTCGTTCTCAAAAAGTCCAACTATCTACAAGAATGGGCTGCGGTCCCTCAAACTTACAAGAAATCCGAGTTATTGGCCATTCAACAACAAGGTCCTAGAGCCATTGGCTTCTTTGGAACCCGAAATATGGGTTTCTTGCATCAAGAACTCATTGAGATTCTTAGCTATGCAATGGTTATAACGAAGAACCACATCTATACTTCAGGAGCATCTGGAACCAATGCAGCAGTTATCAGAGGTGCATTGAGGGCTGAAAAACCAGAACTACTTACTGTCATTTTGCCACAAAGTTTGAAAAAACAACCTCCCGAAAGCCAGGAATTGTTATCCAAAGTTAGGAATGTGATAGAGAAGCCCCACAATGATCATTTATCTTTGATAGAAGCTAGCAGTTTATTGGTAAAGAAAAAGAATTAG

Protein sequence

MSKKFDHKWIEWILGCVKNLRYSIFLNRRPGGRTLATKVIRQGDPLSSFLFILVSEVLDALIENLHQNGLYEGFVVGKDKIHVPILQFADYTLLFANAKATFLDKIQLKLDEWKRFNLSRGGELCFPNGSGDTWKKTLRGARLSGVSMTKIPLIGILPEKKSNALLKDEEISDFQVLLRKLASRNISEVQDRQIWSPGGSRKFTVKSLTLHLSASSPLDKFLYTALWKSNSPRRLCNINWLIVASLPPFARCVYNMKKIANTCSSYAAFRPIVGKGHTFVLKKSNYLQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIEASSLLVKKKN*

Homology

[Close]

BLAST of CSPI07G10400.1 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 3.2e-05
Identity = 28/72 (38.89%), Postives = 38/72 (52.78%), Query Frame = 0


Query: 22 YSIF-LNRRPGGRTLATKVIRQGDPLSSFLFILVSEVLDALIENLHQNGLYEGFVVGKDK 81
          Y +F +N  P G    ++ +RQGDPLS +LFIL +EVL  L     + G   G  V  + 
Sbjct: 9  YLLFIINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNS 68

Query: 82 IHVPILQFADYT 93
            +  L FAD T
Sbjct: 69 PRINHLLFADDT 80

[Close]

BLAST of CSPI07G10400.1 vs. ExPASy TrEMBL
Match: A0A5D3D0Q1 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G00720 PE=4 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 1.5e-85
Identity = 206/397 (51.89%), Postives = 240/397 (60.45%), Query Frame = 0


Query: 1   MSKKFDHKWIEWILGCVKNLRYSIFLNRRPGGRTLATKVIRQGDPLSSFLFILVSEVLDA 60
           MSKKFDHKWIEWILGCVKN RYS+F+NRRP GR LA + IRQGDPLS FLFILVSEVL A
Sbjct: 1   MSKKFDHKWIEWILGCVKNPRYSVFINRRPRGRALAAEEIRQGDPLSPFLFILVSEVLSA 60

Query: 61  LIENLHQNGLYEGFVVGKDKIHVPILQFADYTLLFANAKATFLDKIQLKLDEWKRFNLSR 120
           LIENLHQNGLYEGFVVGKDKIHVPILQFAD TLLF       L  ++  ++    F    
Sbjct: 61  LIENLHQNGLYEGFVVGKDKIHVPILQFADDTLLFCKYDCNMLVTLKQTIEV---FEWCS 120

Query: 121 GGELCFPNGSGDTWKKTLRGARLSGVSMTKIPLIGILPEKKSNALLKDEEISDFQVLLRK 180
             ++   N S D  +  + G +    ++ K  L        S  L+ +E  S  + +LR 
Sbjct: 121 SQKVAKQNTSFDLPRFAIGGGQ---ATLCKSVLSNFPTYYMSIFLMPEEVTSILERILRN 180

Query: 181 LASRNISEVQDRQIWSPGGSRKFTVKSLTLH--LSASSPLDKFLY---TALWKSNSPRRL 240
              +             G      V    +H  L    P    ++   +  W +N     
Sbjct: 181 FFWKG----------HKGSKLNHLVNWKVVHEALMDGGPWPWSIHDKDSFNWHTNGKEGK 240

Query: 241 C-NINWLIVASLPPFARCVYNMKKIANTCSSYAAFRPIVGKGHTFVLKKSNYLQEWAAVP 300
           C   +W+I+      +R    M+ +A+           +G G         +L  W  V 
Sbjct: 241 CLRSSWVII------SRTWLKMEALASFS---------LGNGRRIAF----WLDLWEGVT 300

Query: 301 QTYKKS--ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTN 360
            +   S  ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTN
Sbjct: 301 ISSGLSTVELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTN 360

Query: 361 AAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSK 390
           AAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSK
Sbjct: 361 AAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSK 362

[Close]

BLAST of CSPI07G10400.1 vs. ExPASy TrEMBL
Match: A0A1S3BHE5 (uncharacterized protein LOC103489683 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489683 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 2.4e-51
Identity = 111/124 (89.52%), Postives = 114/124 (91.94%), Query Frame = 0


Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 111 VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 170

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHLSLIE
Sbjct: 171 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVKNVIEKPHNDHLSLIE 230

Query: 407 ASSL 411
           AS L
Sbjct: 231 ASRL 234

[Close]

BLAST of CSPI07G10400.1 vs. ExPASy TrEMBL
Match: A0A1S3BHN4 (uncharacterized protein LOC103489683 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489683 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 2.4e-51
Identity = 111/124 (89.52%), Postives = 114/124 (91.94%), Query Frame = 0


Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 30  VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 89

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHLSLIE
Sbjct: 90  GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVKNVIEKPHNDHLSLIE 149

Query: 407 ASSL 411
           AS L
Sbjct: 150 ASRL 153

[Close]

BLAST of CSPI07G10400.1 vs. ExPASy TrEMBL
Match: A0A6J1DWJ8 (uncharacterized protein LOC111024157 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111024157 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 9.1e-51
Identity = 110/124 (88.71%), Postives = 113/124 (91.13%), Query Frame = 0


Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 119 VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 178

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHL LIE
Sbjct: 179 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVKNVIEKPHNDHLPLIE 238

Query: 407 ASSL 411
           AS L
Sbjct: 239 ASRL 242

[Close]

BLAST of CSPI07G10400.1 vs. ExPASy TrEMBL
Match: A0A6J1DT66 (uncharacterized protein LOC111024157 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111024157 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 9.1e-51
Identity = 110/124 (88.71%), Postives = 113/124 (91.13%), Query Frame = 0


Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 30  VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 89

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHL LIE
Sbjct: 90  GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVKNVIEKPHNDHLPLIE 149

Query: 407 ASSL 411
           AS L
Sbjct: 150 ASRL 153

[Close]

BLAST of CSPI07G10400.1 vs. NCBI nr
Match: KAA0040845.1 (uncharacterized protein E6C27_scaffold333G00680 [Cucumis melo var. makuwa] >TYK17821.1 uncharacterized protein E5676_scaffold306G00720 [Cucumis melo var. makuwa])

HSP 1 Score: 326.6 bits (836), Expect = 3.1e-85
Identity = 206/397 (51.89%), Postives = 240/397 (60.45%), Query Frame = 0


Query: 1   MSKKFDHKWIEWILGCVKNLRYSIFLNRRPGGRTLATKVIRQGDPLSSFLFILVSEVLDA 60
           MSKKFDHKWIEWILGCVKN RYS+F+NRRP GR LA + IRQGDPLS FLFILVSEVL A
Sbjct: 1   MSKKFDHKWIEWILGCVKNPRYSVFINRRPRGRALAAEEIRQGDPLSPFLFILVSEVLSA 60

Query: 61  LIENLHQNGLYEGFVVGKDKIHVPILQFADYTLLFANAKATFLDKIQLKLDEWKRFNLSR 120
           LIENLHQNGLYEGFVVGKDKIHVPILQFAD TLLF       L  ++  ++    F    
Sbjct: 61  LIENLHQNGLYEGFVVGKDKIHVPILQFADDTLLFCKYDCNMLVTLKQTIEV---FEWCS 120

Query: 121 GGELCFPNGSGDTWKKTLRGARLSGVSMTKIPLIGILPEKKSNALLKDEEISDFQVLLRK 180
             ++   N S D  +  + G +    ++ K  L        S  L+ +E  S  + +LR 
Sbjct: 121 SQKVAKQNTSFDLPRFAIGGGQ---ATLCKSVLSNFPTYYMSIFLMPEEVTSILERILRN 180

Query: 181 LASRNISEVQDRQIWSPGGSRKFTVKSLTLH--LSASSPLDKFLY---TALWKSNSPRRL 240
              +             G      V    +H  L    P    ++   +  W +N     
Sbjct: 181 FFWKG----------HKGSKLNHLVNWKVVHEALMDGGPWPWSIHDKDSFNWHTNGKEGK 240

Query: 241 C-NINWLIVASLPPFARCVYNMKKIANTCSSYAAFRPIVGKGHTFVLKKSNYLQEWAAVP 300
           C   +W+I+      +R    M+ +A+           +G G         +L  W  V 
Sbjct: 241 CLRSSWVII------SRTWLKMEALASFS---------LGNGRRIAF----WLDLWEGVT 300

Query: 301 QTYKKS--ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTN 360
            +   S  ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTN
Sbjct: 301 ISSGLSTVELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTN 360

Query: 361 AAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSK 390
           AAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSK
Sbjct: 361 AAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSK 362

[Close]

BLAST of CSPI07G10400.1 vs. NCBI nr
Match: XP_011659013.1 (uncharacterized protein LOC101209189 isoform X2 [Cucumis sativus])

HSP 1 Score: 214.2 bits (544), Expect = 2.2e-51
Identity = 112/124 (90.32%), Postives = 114/124 (91.94%), Query Frame = 0


Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 32  VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 91

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE
Sbjct: 92  GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 151

Query: 407 ASSL 411
           AS L
Sbjct: 152 ASRL 155

[Close]

BLAST of CSPI07G10400.1 vs. NCBI nr
Match: XP_004139811.1 (uncharacterized protein LOC101209189 isoform X1 [Cucumis sativus] >KAE8646086.1 hypothetical protein Csa_016304 [Cucumis sativus])

HSP 1 Score: 214.2 bits (544), Expect = 2.2e-51
Identity = 112/124 (90.32%), Postives = 114/124 (91.94%), Query Frame = 0


Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 109 VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 168

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE
Sbjct: 169 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 228

Query: 407 ASSL 411
           AS L
Sbjct: 229 ASRL 232

[Close]

BLAST of CSPI07G10400.1 vs. NCBI nr
Match: XP_008447166.1 (PREDICTED: uncharacterized protein LOC103489683 isoform X1 [Cucumis melo])

HSP 1 Score: 213.0 bits (541), Expect = 4.9e-51
Identity = 111/124 (89.52%), Postives = 114/124 (91.94%), Query Frame = 0


Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 111 VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 170

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHLSLIE
Sbjct: 171 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVKNVIEKPHNDHLSLIE 230

Query: 407 ASSL 411
           AS L
Sbjct: 231 ASRL 234

[Close]

BLAST of CSPI07G10400.1 vs. NCBI nr
Match: XP_038888205.1 (uncharacterized protein LOC120078074 isoform X2 [Benincasa hispida])

HSP 1 Score: 213.0 bits (541), Expect = 4.9e-51
Identity = 111/124 (89.52%), Postives = 114/124 (91.94%), Query Frame = 0


Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 39  VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 98

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHLSLIE
Sbjct: 99  GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVKNVIEKPHNDHLSLIE 158

Query: 407 ASSL 411
           AS L
Sbjct: 159 ASRL 162

[Close]

BLAST of CSPI07G10400.1 vs. TAIR 10
Match: AT2G43945.1 (unknown protein; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G59870.1); Has 292 Blast hits to 292 proteins in 84 species: Archae - 0; Bacteria - 122; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 124 (source: NCBI BLink). )

HSP 1 Score: 203.0 bits (515), Expect = 4.7e-52
Identity = 102/110 (92.73%), Postives = 108/110 (98.18%), Query Frame = 0


Query: 301 ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTNAAVIRGAL 360
           ELLAIQQQGPR+IGFFGTRNMGF+HQELIEILSYAMVITKNHIYTSGASGTNAAVIRGAL
Sbjct: 137 ELLAIQQQGPRSIGFFGTRNMGFMHQELIEILSYAMVITKNHIYTSGASGTNAAVIRGAL 196

Query: 361 RAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIEASSL 411
           RAE+PELLTVILPQSLKKQPPESQELLSKV+NV+EKPHNDHL L+EAS L
Sbjct: 197 RAERPELLTVILPQSLKKQPPESQELLSKVQNVVEKPHNDHLPLLEASRL 246

[Close]

BLAST of CSPI07G10400.1 vs. TAIR 10
Match: AT3G59870.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G43945.1); Has 292 Blast hits to 292 proteins in 84 species: Archae - 0; Bacteria - 122; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 124 (source: NCBI BLink). )

HSP 1 Score: 202.6 bits (514), Expect = 6.2e-52
Identity = 104/124 (83.87%), Postives = 111/124 (89.52%), Query Frame = 0


Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E   VP      ELLAIQQQGPR IGFFGTRNMGF+HQELI+ILSYAMVITKNHIYTS
Sbjct: 122 VSELQRVPDVDYIQELLAIQQQGPRTIGFFGTRNMGFMHQELIQILSYAMVITKNHIYTS 181

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GA+GTNAAVIRGALRAE+PELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHL L+E
Sbjct: 182 GAAGTNAAVIRGALRAERPELLTVILPQSLKKQPPESQELLSKVQNVIEKPHNDHLPLLE 241

Query: 407 ASSL 411
           AS L
Sbjct: 242 ASRL 245

[Close]

BLAST of CSPI07G10400.1 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 51.2 bits (121), Expect = 2.3e-06
Identity = 28/72 (38.89%), Postives = 38/72 (52.78%), Query Frame = 0


Query: 22 YSIF-LNRRPGGRTLATKVIRQGDPLSSFLFILVSEVLDALIENLHQNGLYEGFVVGKDK 81
          Y +F +N  P G    ++ +RQGDPLS +LFIL +EVL  L     + G   G  V  + 
Sbjct: 9  YLLFIINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNS 68

Query: 82 IHVPILQFADYT 93
            +  L FAD T
Sbjct: 69 PRINHLLFADDT 80

The following BLAST results are available for this feature:

BLAST of CSPI07G10400.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P92555	3.2e-05	38.89	Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of CSPI07G10400.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D0Q1	1.5e-85	51.89	Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3BHE5	2.4e-51	89.52	uncharacterized protein LOC103489683 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A1S3BHN4	2.4e-51	89.52	uncharacterized protein LOC103489683 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1DWJ8	9.1e-51	88.71	uncharacterized protein LOC111024157 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DT66	9.1e-51	88.71	uncharacterized protein LOC111024157 isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of CSPI07G10400.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0040845.1	3.1e-85	51.89	uncharacterized protein E6C27_scaffold333G00680 [Cucumis melo var. makuwa] >TYK1...	[more]
XP_011659013.1	2.2e-51	90.32	uncharacterized protein LOC101209189 isoform X2 [Cucumis sativus]	[more]
XP_004139811.1	2.2e-51	90.32	uncharacterized protein LOC101209189 isoform X1 [Cucumis sativus] >KAE8646086.1 ...	[more]
XP_008447166.1	4.9e-51	89.52	PREDICTED: uncharacterized protein LOC103489683 isoform X1 [Cucumis melo]	[more]
XP_038888205.1	4.9e-51	89.52	uncharacterized protein LOC120078074 isoform X2 [Benincasa hispida]	[more]

BLAST of CSPI07G10400.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G43945.1	4.7e-52	92.73	unknown protein; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein matc...	[more]
AT3G59870.1	6.2e-52	83.87	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
ATMG01250.1	2.3e-06	38.89	RNA-directed DNA polymerase (reverse transcriptase)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 4..113 e-value: 2.2E-7 score: 30.6
None	No IPR available	PANTHER	PTHR32183:SF6	CYANOBACTERIA-SPECIFIC PROTEIN-LIKE	coord: 298..410
None	No IPR available	SUPERFAMILY	102405	MCP/YpsA-like	coord: 305..382
IPR044995	Thiocyanate methyltransferase/thiol methyltransferase	PANTHER	PTHR32183	FAMILY NOT NAMED	coord: 298..410

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI07G10400	CSPI07G10400	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI07G10400.1.cds1	CSPI07G10400.1.cds1	CDS
CSPI07G10400.1.cds2	CSPI07G10400.1.cds2	CDS
CSPI07G10400.1.cds3	CSPI07G10400.1.cds3	CDS
CSPI07G10400.1.cds4	CSPI07G10400.1.cds4	CDS
CSPI07G10400.1.cds5	CSPI07G10400.1.cds5	CDS
CSPI07G10400.1.cds6	CSPI07G10400.1.cds6	CDS
CSPI07G10400.1.cds7	CSPI07G10400.1.cds7	CDS
CSPI07G10400.1.cds8	CSPI07G10400.1.cds8	CDS
CSPI07G10400.1.cds9	CSPI07G10400.1.cds9	CDS
CSPI07G10400.1.cds10	CSPI07G10400.1.cds10	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI07G10400.1	CSPI07G10400.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity