CSPI07G10400 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G10400
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionReverse transcriptase domain-containing protein
LocationChr7: 8501993 .. 8506164 (+)
RNA-Seq ExpressionCSPI07G10400
SyntenyCSPI07G10400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTAAGAAGTTTGACCACAAGTGGATTGAATGGATCTTGGGTTGTGTTAAGAACCTGAGATACTCAATATTCCTCAATAGAAGACCGGGAGGAAGAACGTTGGCTACAAAGGTTATCAGGCAGGGTGACCCCCTTTCGTCGTTCCTCTTCATACTTGTAAGTGAAGTTCTTGATGCTCTAATTGAAAATCTACATCAAAATGGCCTTTATGAAGGTTTTGTAGTGGGAAAGGACAAGATTCATGTTCCAATCCTTCAATTTGCAGATTACACCCTACTTTTTGCAAATGCGAAAGCAACATGTTAGTAACTTTGAAACAGACAATAGAGCTTTTTGAATGGTGCTCCTGGCAAAAAGTGAACTGGGACAAGTCGGCCCTTTTTAGATAAACGTGGAAGAGATATCCTCTTTTTGGCAACCAGTTCTTGATAAAATTCAACTAAAGTTAGATGAATGGAAGAGGTTTAACCTCTCGAGAGGGGGGGAGCTATGCTTTGTAAGTCTATATTCCTAATGCCAGAAGAAGTCACTTCAAAATTGGAAAGAATCCTGAGGAATTTCTTTTGGAAGGGAGGGCAGCAAATTAAACTACCTTGTTAGTTAGAAAGTGGTCAACAAATTCTTGATGGATGGTGGCCCTTGGCCTTGGTGGCTTCAAATCTAAAAACTTGGCGCTTCTAGCCAAATGGGAGTGGAGATACATGGAAGAAGACTCTCCGTGGTGCAAGGTTATCAGGAGTATCCATGACAAAGATTCCTTTAATCGGCATACTGCCAGAAAAGAAGTCAAATGCGTGAGAAGCCCTTGGGTTATTATTTCGAGAACTTGGTTGAAAATGGAATCTTTAGCCACCTTTAGCTTGGGAATGGGAGAAGTATAGCTTTTTGGTTAGATCACTTGGGGGAGTGACCATTTCATCTAGCCTTTCCACAGTTGTTCAGAATAGCTTTACTCCCAAATGTTTCGGTTGCTGATCGCTGAGACTCTGCATTATCATCTTGGTCCCTAATCTTCTGCAGTTTGCTAAAAGATGAAGAGATTTCAGACTTCCAAGTACTGCTTCGAAAATTAGCATCAAGAAATATTTCTGAAGTTCAAGACAGACAAATATGGTCACCTGGCGGCAGCAGAAAATTTACAGTAAAGTCCCTTACTCTTCACCTGTCTGCTTCCTCCCCTCTGGATAAATTCCTCTATACGGCCCTTTGGAAGTCTAATAGCCCAAGAAGGGTTAATATCTTCATTTGGATCACCCTGATAGGTTAACTAAATGTGTCCTCAGCTTTGCAACATAAATTGGCTAATAGTTGCCTCTCTCCCTCCATTTGCCCGCTGTGTCTACAACATGAAGAAGATTGCCAACACTTGTTCTTCCTATGCAGCTTTTCGGCCAATTGTTGGGAAAGTCTCCTCAACTTCTTTAATATTGGGCGTTTGGTGCAACTTTTTGTGATAATGTGATTCCTCTGTTAATCGGCTCCTGTCTAAATAGAGAAACTTCTTTACTTTGGGTTAACACGGTGAAAGCTATACTGGCAGAAATCTGGTTTGAGAGGAATATAGGGTGTTCCATGATAAATCTCTTGATTGGAAGGATCACTTTAGAAACGCGCATCAAAATACCTCCTCATGGTGTTCGGCCTCAAAGATCTTCAGCAGCTACAGCATCCAAGGGCTCAACTTAAATTGGAGTGCCTTCATTTTTCTCCAGCTTGATTATTCCCCAATGTGTTTGCGCTTCTGTTTTTTTCTTTTTTTGGTCGTACGCAATCTTTATAGCTTTCCTCCTTTGTTTTGGAATCTCTTTCTTCTCCTACTAGTTATCTTGTAAGCACTTGTTTAACTCTTTTGTACATTGAACTTTTTGCCTCATCACGAATATTAACAAAGCTACTCGTTTCCATTTAAAAAAAGGTGTACAGTTTTATCTCTTTGCAAATGGTACAATTTTCAAGGAAACAATTTTTTTCTTTTGGGGGGGGGGTAAGAAATCAAGTTTTCTTTGATAAAAGTTTATACAGGGACATACATTCGTTCTCAAAAAGTCCAACTATCTACAAGAATGGGCTGCGGTCCCTCAAACTTACAAGAAATCCGTTCTTACCCGAGGAAACAAATCTTTTGTTAACCTTTCTGTCACATCTCTTAGTTAGCCAGTGCACACAATTTCTCAATTATAGTTCTTGGTTTGAAGCTCAGCTCCTTCCAAATTACAATTTTTTTTATTTACTTTATTATTGATTATCATTTTTTTTTCCATTTGCAGGAGTTATTGGCCATTCAACAACAAGGTCCTAGAGCCATTGGCTTCTTTGGAACCCGAAATATGGGTTTCTTGCATCAAGAACTCATTGAGATTCTTAGCTATGCAATGGTTATAACGGTAGAATATGTGATTTATTGTCGCTGCACTATTGAAACGCTATTTAGTCTTTCATGACTGCAAAATATTCATATTGCTTAGTATATTCTGTATGCAGAAGAACCACATCTATACTTCAGGAGCATCTGGAACCAATGCAGCAGTTATCAGAGGTGCATTGAGGGCTGAAAAACCAGAACTACTTACTGTCATTTTGCCACAAAGTTTGAAAAAACAACCTCCCGAAAGCCAGGAATTGTTATCCAAAGTAAGTTTGATTGGTTATAAACATATTTACTCAAGAGTTAGAGCTTGTATATGTGTACTGTTTGATTGGTTTAGTCAAGTTTCCGTTTTGAGTTATTTAAAATGGCTTAGGGGGGCATTTGTTGCACAAAGTTGAGTTAGTAATTAAATTATTTAGAGTGTAGAGTTAAGTTGAGTTAGAGTATCTGGTGTAATATTTCTAAAGGATTCTGATTTTTGTTTTTTTCTAAATTTTTTTGTGCAAGTATTAAATTGCTGCATATTCTTCTAAAACTTTCAGTACTCAAATTACATTAACATAATCTTTTCCATTCTTAAAAAGTTATTTTGAACACTCATTTCCTTGACATATTTTTGCTCAATTCTTAAAAGCTTGAGATATCCTAAACACTAATTTTGGACACCTAATTACTTAAAAACTTAGCATATCTTTTTTCCATTCTTAAAAACTCAACATATTCTTGAAAACTTAGCATATTCTGAACACAAGTTTTCATTGCTCAATTACTTTAAGACGTGGTATGTTATTGTATTTCATTTTCAAATGAAATTTAATGCAAAATTAAGTCACAATACACCAAAATTGATTCACACTCAAATACTTAAAAATTTCATTCATACTCGAAGAGAGAAATTTGTGGTACATTTCTTTTTTCTATTTTAGGGCTATTTAGGGTTTTTTTTTGCCATGATACTGAAATTATGATTTTTAAAACGTTTTTTTTTACTTCAAAACAATTTAGCTGATTTCAAAACAATTTTATTTATTTTCAAAAAGTTTTTAGTTCAAAACAGTTCTGTTTACTTTTATGTCAAATTACTAGTTTTGATATAGTGCAAACATTTTCTTAAAAATCCTTCCATTTCAAACAGCACGATAGTACAGTAAGTGTACTGTTAGGAGAGGCTCTCAACTGTTGCAAAATCACTGGTCTGACTCGTAATAGAGAATGAATTTAATAGAACAGTGTTTGTTTTTATTCTGTTTTGTGTGTGTGCTGTTCTGGTGTTTTCCAGGTGCTACTAAGTTGGATTTTTAACAAAAAACTTCGGAAATCAAATGTAGTTTCGTTGCACTTGAAAATATCACAAGTTATTTTGTTAAGAGTTTTTACAGTGTGCTTTTAGAGTCAGAAAATAGTTTAAACACCATTTCTAAATCTTCCCCAACTTTTGATTGTCTATATCAACATTCTCTGTTAATATTATAGAGAATGAATTGTAAGGGCCCTTCAGTCAACTTTTTTTTTTTTTTTAAATTGTTCCTTTGGTCAACTGCTTAGATGATCTATTGTTGATGACTTCATTTGGATAAGTCTTTATTAAATTTTTTTTCTATTTTGTTTATCTTGGAATTTGTAGGTTAGGAATGTGATAGAGAAGCCCCACAATGATCATTTATCTTTGATAGAAGCTAGCAGGTATGTGTGTAGTTTTTTTCTTAGTTGACCATAAGGTGAATTAGAATTTCACATAGTGAAAATATTTTTACTGATGTTGATTTTTAATAATAGTTTATTGGTAAAGAAAAAGAATTAG

mRNA sequence

ATGAGTAAGAAGTTTGACCACAAGTGGATTGAATGGATCTTGGGTTGTGTTAAGAACCTGAGATACTCAATATTCCTCAATAGAAGACCGGGAGGAAGAACGTTGGCTACAAAGGTTATCAGGCAGGGTGACCCCCTTTCGTCGTTCCTCTTCATACTTGTAAGTGAAGTTCTTGATGCTCTAATTGAAAATCTACATCAAAATGGCCTTTATGAAGGTTTTGTAGTGGGAAAGGACAAGATTCATGTTCCAATCCTTCAATTTGCAGATTACACCCTACTTTTTGCAAATGCGAAAGCAACATTTCTTGATAAAATTCAACTAAAGTTAGATGAATGGAAGAGGTTTAACCTCTCGAGAGGGGGGGAGCTATGCTTTCCAAATGGGAGTGGAGATACATGGAAGAAGACTCTCCGTGGTGCAAGGTTATCAGGAGTATCCATGACAAAGATTCCTTTAATCGGCATACTGCCAGAAAAGAAGTCAAATGCTTTGCTAAAAGATGAAGAGATTTCAGACTTCCAAGTACTGCTTCGAAAATTAGCATCAAGAAATATTTCTGAAGTTCAAGACAGACAAATATGGTCACCTGGCGGCAGCAGAAAATTTACAGTAAAGTCCCTTACTCTTCACCTGTCTGCTTCCTCCCCTCTGGATAAATTCCTCTATACGGCCCTTTGGAAGTCTAATAGCCCAAGAAGGCTTTGCAACATAAATTGGCTAATAGTTGCCTCTCTCCCTCCATTTGCCCGCTGTGTCTACAACATGAAGAAGATTGCCAACACTTGTTCTTCCTATGCAGCTTTTCGGCCAATTGTTGGGAAAGGACATACATTCGTTCTCAAAAAGTCCAACTATCTACAAGAATGGGCTGCGGTCCCTCAAACTTACAAGAAATCCGAGTTATTGGCCATTCAACAACAAGGTCCTAGAGCCATTGGCTTCTTTGGAACCCGAAATATGGGTTTCTTGCATCAAGAACTCATTGAGATTCTTAGCTATGCAATGGTTATAACGAAGAACCACATCTATACTTCAGGAGCATCTGGAACCAATGCAGCAGTTATCAGAGGTGCATTGAGGGCTGAAAAACCAGAACTACTTACTGTCATTTTGCCACAAAGTTTGAAAAAACAACCTCCCGAAAGCCAGGAATTGTTATCCAAAGTTAGGAATGTGATAGAGAAGCCCCACAATGATCATTTATCTTTGATAGAAGCTAGCAGTTTATTGGTAAAGAAAAAGAATTAG

Coding sequence (CDS)

ATGAGTAAGAAGTTTGACCACAAGTGGATTGAATGGATCTTGGGTTGTGTTAAGAACCTGAGATACTCAATATTCCTCAATAGAAGACCGGGAGGAAGAACGTTGGCTACAAAGGTTATCAGGCAGGGTGACCCCCTTTCGTCGTTCCTCTTCATACTTGTAAGTGAAGTTCTTGATGCTCTAATTGAAAATCTACATCAAAATGGCCTTTATGAAGGTTTTGTAGTGGGAAAGGACAAGATTCATGTTCCAATCCTTCAATTTGCAGATTACACCCTACTTTTTGCAAATGCGAAAGCAACATTTCTTGATAAAATTCAACTAAAGTTAGATGAATGGAAGAGGTTTAACCTCTCGAGAGGGGGGGAGCTATGCTTTCCAAATGGGAGTGGAGATACATGGAAGAAGACTCTCCGTGGTGCAAGGTTATCAGGAGTATCCATGACAAAGATTCCTTTAATCGGCATACTGCCAGAAAAGAAGTCAAATGCTTTGCTAAAAGATGAAGAGATTTCAGACTTCCAAGTACTGCTTCGAAAATTAGCATCAAGAAATATTTCTGAAGTTCAAGACAGACAAATATGGTCACCTGGCGGCAGCAGAAAATTTACAGTAAAGTCCCTTACTCTTCACCTGTCTGCTTCCTCCCCTCTGGATAAATTCCTCTATACGGCCCTTTGGAAGTCTAATAGCCCAAGAAGGCTTTGCAACATAAATTGGCTAATAGTTGCCTCTCTCCCTCCATTTGCCCGCTGTGTCTACAACATGAAGAAGATTGCCAACACTTGTTCTTCCTATGCAGCTTTTCGGCCAATTGTTGGGAAAGGACATACATTCGTTCTCAAAAAGTCCAACTATCTACAAGAATGGGCTGCGGTCCCTCAAACTTACAAGAAATCCGAGTTATTGGCCATTCAACAACAAGGTCCTAGAGCCATTGGCTTCTTTGGAACCCGAAATATGGGTTTCTTGCATCAAGAACTCATTGAGATTCTTAGCTATGCAATGGTTATAACGAAGAACCACATCTATACTTCAGGAGCATCTGGAACCAATGCAGCAGTTATCAGAGGTGCATTGAGGGCTGAAAAACCAGAACTACTTACTGTCATTTTGCCACAAAGTTTGAAAAAACAACCTCCCGAAAGCCAGGAATTGTTATCCAAAGTTAGGAATGTGATAGAGAAGCCCCACAATGATCATTTATCTTTGATAGAAGCTAGCAGTTTATTGGTAAAGAAAAAGAATTAG

Protein sequence

MSKKFDHKWIEWILGCVKNLRYSIFLNRRPGGRTLATKVIRQGDPLSSFLFILVSEVLDALIENLHQNGLYEGFVVGKDKIHVPILQFADYTLLFANAKATFLDKIQLKLDEWKRFNLSRGGELCFPNGSGDTWKKTLRGARLSGVSMTKIPLIGILPEKKSNALLKDEEISDFQVLLRKLASRNISEVQDRQIWSPGGSRKFTVKSLTLHLSASSPLDKFLYTALWKSNSPRRLCNINWLIVASLPPFARCVYNMKKIANTCSSYAAFRPIVGKGHTFVLKKSNYLQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIEASSLLVKKKN*
Homology
BLAST of CSPI07G10400 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 3.2e-05
Identity = 28/72 (38.89%), Postives = 38/72 (52.78%), Query Frame = 0

Query: 22 YSIF-LNRRPGGRTLATKVIRQGDPLSSFLFILVSEVLDALIENLHQNGLYEGFVVGKDK 81
          Y +F +N  P G    ++ +RQGDPLS +LFIL +EVL  L     + G   G  V  + 
Sbjct: 9  YLLFIINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNS 68

Query: 82 IHVPILQFADYT 93
            +  L FAD T
Sbjct: 69 PRINHLLFADDT 80

BLAST of CSPI07G10400 vs. ExPASy TrEMBL
Match: A0A5D3D0Q1 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G00720 PE=4 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 1.5e-85
Identity = 206/397 (51.89%), Postives = 240/397 (60.45%), Query Frame = 0

Query: 1   MSKKFDHKWIEWILGCVKNLRYSIFLNRRPGGRTLATKVIRQGDPLSSFLFILVSEVLDA 60
           MSKKFDHKWIEWILGCVKN RYS+F+NRRP GR LA + IRQGDPLS FLFILVSEVL A
Sbjct: 1   MSKKFDHKWIEWILGCVKNPRYSVFINRRPRGRALAAEEIRQGDPLSPFLFILVSEVLSA 60

Query: 61  LIENLHQNGLYEGFVVGKDKIHVPILQFADYTLLFANAKATFLDKIQLKLDEWKRFNLSR 120
           LIENLHQNGLYEGFVVGKDKIHVPILQFAD TLLF       L  ++  ++    F    
Sbjct: 61  LIENLHQNGLYEGFVVGKDKIHVPILQFADDTLLFCKYDCNMLVTLKQTIEV---FEWCS 120

Query: 121 GGELCFPNGSGDTWKKTLRGARLSGVSMTKIPLIGILPEKKSNALLKDEEISDFQVLLRK 180
             ++   N S D  +  + G +    ++ K  L        S  L+ +E  S  + +LR 
Sbjct: 121 SQKVAKQNTSFDLPRFAIGGGQ---ATLCKSVLSNFPTYYMSIFLMPEEVTSILERILRN 180

Query: 181 LASRNISEVQDRQIWSPGGSRKFTVKSLTLH--LSASSPLDKFLY---TALWKSNSPRRL 240
              +             G      V    +H  L    P    ++   +  W +N     
Sbjct: 181 FFWKG----------HKGSKLNHLVNWKVVHEALMDGGPWPWSIHDKDSFNWHTNGKEGK 240

Query: 241 C-NINWLIVASLPPFARCVYNMKKIANTCSSYAAFRPIVGKGHTFVLKKSNYLQEWAAVP 300
           C   +W+I+      +R    M+ +A+           +G G         +L  W  V 
Sbjct: 241 CLRSSWVII------SRTWLKMEALASFS---------LGNGRRIAF----WLDLWEGVT 300

Query: 301 QTYKKS--ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTN 360
            +   S  ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTN
Sbjct: 301 ISSGLSTVELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTN 360

Query: 361 AAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSK 390
           AAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSK
Sbjct: 361 AAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSK 362

BLAST of CSPI07G10400 vs. ExPASy TrEMBL
Match: A0A1S3BHE5 (uncharacterized protein LOC103489683 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489683 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 2.4e-51
Identity = 111/124 (89.52%), Postives = 114/124 (91.94%), Query Frame = 0

Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 111 VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 170

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHLSLIE
Sbjct: 171 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVKNVIEKPHNDHLSLIE 230

Query: 407 ASSL 411
           AS L
Sbjct: 231 ASRL 234

BLAST of CSPI07G10400 vs. ExPASy TrEMBL
Match: A0A1S3BHN4 (uncharacterized protein LOC103489683 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489683 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 2.4e-51
Identity = 111/124 (89.52%), Postives = 114/124 (91.94%), Query Frame = 0

Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 30  VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 89

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHLSLIE
Sbjct: 90  GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVKNVIEKPHNDHLSLIE 149

Query: 407 ASSL 411
           AS L
Sbjct: 150 ASRL 153

BLAST of CSPI07G10400 vs. ExPASy TrEMBL
Match: A0A6J1DWJ8 (uncharacterized protein LOC111024157 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111024157 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 9.1e-51
Identity = 110/124 (88.71%), Postives = 113/124 (91.13%), Query Frame = 0

Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 119 VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 178

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHL LIE
Sbjct: 179 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVKNVIEKPHNDHLPLIE 238

Query: 407 ASSL 411
           AS L
Sbjct: 239 ASRL 242

BLAST of CSPI07G10400 vs. ExPASy TrEMBL
Match: A0A6J1DT66 (uncharacterized protein LOC111024157 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111024157 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 9.1e-51
Identity = 110/124 (88.71%), Postives = 113/124 (91.13%), Query Frame = 0

Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 30  VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 89

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHL LIE
Sbjct: 90  GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVKNVIEKPHNDHLPLIE 149

Query: 407 ASSL 411
           AS L
Sbjct: 150 ASRL 153

BLAST of CSPI07G10400 vs. NCBI nr
Match: KAA0040845.1 (uncharacterized protein E6C27_scaffold333G00680 [Cucumis melo var. makuwa] >TYK17821.1 uncharacterized protein E5676_scaffold306G00720 [Cucumis melo var. makuwa])

HSP 1 Score: 326.6 bits (836), Expect = 3.1e-85
Identity = 206/397 (51.89%), Postives = 240/397 (60.45%), Query Frame = 0

Query: 1   MSKKFDHKWIEWILGCVKNLRYSIFLNRRPGGRTLATKVIRQGDPLSSFLFILVSEVLDA 60
           MSKKFDHKWIEWILGCVKN RYS+F+NRRP GR LA + IRQGDPLS FLFILVSEVL A
Sbjct: 1   MSKKFDHKWIEWILGCVKNPRYSVFINRRPRGRALAAEEIRQGDPLSPFLFILVSEVLSA 60

Query: 61  LIENLHQNGLYEGFVVGKDKIHVPILQFADYTLLFANAKATFLDKIQLKLDEWKRFNLSR 120
           LIENLHQNGLYEGFVVGKDKIHVPILQFAD TLLF       L  ++  ++    F    
Sbjct: 61  LIENLHQNGLYEGFVVGKDKIHVPILQFADDTLLFCKYDCNMLVTLKQTIEV---FEWCS 120

Query: 121 GGELCFPNGSGDTWKKTLRGARLSGVSMTKIPLIGILPEKKSNALLKDEEISDFQVLLRK 180
             ++   N S D  +  + G +    ++ K  L        S  L+ +E  S  + +LR 
Sbjct: 121 SQKVAKQNTSFDLPRFAIGGGQ---ATLCKSVLSNFPTYYMSIFLMPEEVTSILERILRN 180

Query: 181 LASRNISEVQDRQIWSPGGSRKFTVKSLTLH--LSASSPLDKFLY---TALWKSNSPRRL 240
              +             G      V    +H  L    P    ++   +  W +N     
Sbjct: 181 FFWKG----------HKGSKLNHLVNWKVVHEALMDGGPWPWSIHDKDSFNWHTNGKEGK 240

Query: 241 C-NINWLIVASLPPFARCVYNMKKIANTCSSYAAFRPIVGKGHTFVLKKSNYLQEWAAVP 300
           C   +W+I+      +R    M+ +A+           +G G         +L  W  V 
Sbjct: 241 CLRSSWVII------SRTWLKMEALASFS---------LGNGRRIAF----WLDLWEGVT 300

Query: 301 QTYKKS--ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTN 360
            +   S  ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTN
Sbjct: 301 ISSGLSTVELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTN 360

Query: 361 AAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSK 390
           AAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSK
Sbjct: 361 AAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSK 362

BLAST of CSPI07G10400 vs. NCBI nr
Match: XP_011659013.1 (uncharacterized protein LOC101209189 isoform X2 [Cucumis sativus])

HSP 1 Score: 214.2 bits (544), Expect = 2.2e-51
Identity = 112/124 (90.32%), Postives = 114/124 (91.94%), Query Frame = 0

Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 32  VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 91

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE
Sbjct: 92  GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 151

Query: 407 ASSL 411
           AS L
Sbjct: 152 ASRL 155

BLAST of CSPI07G10400 vs. NCBI nr
Match: XP_004139811.1 (uncharacterized protein LOC101209189 isoform X1 [Cucumis sativus] >KAE8646086.1 hypothetical protein Csa_016304 [Cucumis sativus])

HSP 1 Score: 214.2 bits (544), Expect = 2.2e-51
Identity = 112/124 (90.32%), Postives = 114/124 (91.94%), Query Frame = 0

Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 109 VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 168

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE
Sbjct: 169 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 228

Query: 407 ASSL 411
           AS L
Sbjct: 229 ASRL 232

BLAST of CSPI07G10400 vs. NCBI nr
Match: XP_008447166.1 (PREDICTED: uncharacterized protein LOC103489683 isoform X1 [Cucumis melo])

HSP 1 Score: 213.0 bits (541), Expect = 4.9e-51
Identity = 111/124 (89.52%), Postives = 114/124 (91.94%), Query Frame = 0

Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 111 VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 170

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHLSLIE
Sbjct: 171 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVKNVIEKPHNDHLSLIE 230

Query: 407 ASSL 411
           AS L
Sbjct: 231 ASRL 234

BLAST of CSPI07G10400 vs. NCBI nr
Match: XP_038888205.1 (uncharacterized protein LOC120078074 isoform X2 [Benincasa hispida])

HSP 1 Score: 213.0 bits (541), Expect = 4.9e-51
Identity = 111/124 (89.52%), Postives = 114/124 (91.94%), Query Frame = 0

Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E+  VP      ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS
Sbjct: 39  VSEFKPVPDVDYLQELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 98

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHLSLIE
Sbjct: 99  GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVKNVIEKPHNDHLSLIE 158

Query: 407 ASSL 411
           AS L
Sbjct: 159 ASRL 162

BLAST of CSPI07G10400 vs. TAIR 10
Match: AT2G43945.1 (unknown protein; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G59870.1); Has 292 Blast hits to 292 proteins in 84 species: Archae - 0; Bacteria - 122; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 124 (source: NCBI BLink). )

HSP 1 Score: 203.0 bits (515), Expect = 4.7e-52
Identity = 102/110 (92.73%), Postives = 108/110 (98.18%), Query Frame = 0

Query: 301 ELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTSGASGTNAAVIRGAL 360
           ELLAIQQQGPR+IGFFGTRNMGF+HQELIEILSYAMVITKNHIYTSGASGTNAAVIRGAL
Sbjct: 137 ELLAIQQQGPRSIGFFGTRNMGFMHQELIEILSYAMVITKNHIYTSGASGTNAAVIRGAL 196

Query: 361 RAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIEASSL 411
           RAE+PELLTVILPQSLKKQPPESQELLSKV+NV+EKPHNDHL L+EAS L
Sbjct: 197 RAERPELLTVILPQSLKKQPPESQELLSKVQNVVEKPHNDHLPLLEASRL 246

BLAST of CSPI07G10400 vs. TAIR 10
Match: AT3G59870.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G43945.1); Has 292 Blast hits to 292 proteins in 84 species: Archae - 0; Bacteria - 122; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 124 (source: NCBI BLink). )

HSP 1 Score: 202.6 bits (514), Expect = 6.2e-52
Identity = 104/124 (83.87%), Postives = 111/124 (89.52%), Query Frame = 0

Query: 287 LQEWAAVPQTYKKSELLAIQQQGPRAIGFFGTRNMGFLHQELIEILSYAMVITKNHIYTS 346
           + E   VP      ELLAIQQQGPR IGFFGTRNMGF+HQELI+ILSYAMVITKNHIYTS
Sbjct: 122 VSELQRVPDVDYIQELLAIQQQGPRTIGFFGTRNMGFMHQELIQILSYAMVITKNHIYTS 181

Query: 347 GASGTNAAVIRGALRAEKPELLTVILPQSLKKQPPESQELLSKVRNVIEKPHNDHLSLIE 406
           GA+GTNAAVIRGALRAE+PELLTVILPQSLKKQPPESQELLSKV+NVIEKPHNDHL L+E
Sbjct: 182 GAAGTNAAVIRGALRAERPELLTVILPQSLKKQPPESQELLSKVQNVIEKPHNDHLPLLE 241

Query: 407 ASSL 411
           AS L
Sbjct: 242 ASRL 245

BLAST of CSPI07G10400 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 51.2 bits (121), Expect = 2.3e-06
Identity = 28/72 (38.89%), Postives = 38/72 (52.78%), Query Frame = 0

Query: 22 YSIF-LNRRPGGRTLATKVIRQGDPLSSFLFILVSEVLDALIENLHQNGLYEGFVVGKDK 81
          Y +F +N  P G    ++ +RQGDPLS +LFIL +EVL  L     + G   G  V  + 
Sbjct: 9  YLLFIINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNS 68

Query: 82 IHVPILQFADYT 93
            +  L FAD T
Sbjct: 69 PRINHLLFADDT 80

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P925553.2e-0538.89Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A5D3D0Q11.5e-8551.89Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3BHE52.4e-5189.52uncharacterized protein LOC103489683 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A1S3BHN42.4e-5189.52uncharacterized protein LOC103489683 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1DWJ89.1e-5188.71uncharacterized protein LOC111024157 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1DT669.1e-5188.71uncharacterized protein LOC111024157 isoform X2 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
KAA0040845.13.1e-8551.89uncharacterized protein E6C27_scaffold333G00680 [Cucumis melo var. makuwa] >TYK1... [more]
XP_011659013.12.2e-5190.32uncharacterized protein LOC101209189 isoform X2 [Cucumis sativus][more]
XP_004139811.12.2e-5190.32uncharacterized protein LOC101209189 isoform X1 [Cucumis sativus] >KAE8646086.1 ... [more]
XP_008447166.14.9e-5189.52PREDICTED: uncharacterized protein LOC103489683 isoform X1 [Cucumis melo][more]
XP_038888205.14.9e-5189.52uncharacterized protein LOC120078074 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G43945.14.7e-5292.73unknown protein; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein matc... [more]
AT3G59870.16.2e-5283.87unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
ATMG01250.12.3e-0638.89RNA-directed DNA polymerase (reverse transcriptase) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 4..113
e-value: 2.2E-7
score: 30.6
NoneNo IPR availablePANTHERPTHR32183:SF6CYANOBACTERIA-SPECIFIC PROTEIN-LIKEcoord: 298..410
NoneNo IPR availableSUPERFAMILY102405MCP/YpsA-likecoord: 305..382
IPR044995Thiocyanate methyltransferase/thiol methyltransferasePANTHERPTHR32183FAMILY NOT NAMEDcoord: 298..410

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G10400.1CSPI07G10400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity