CSPI04G22410.2 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI04G22410.2
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Peptide-methionine (R)-S-oxide reductase
Location	Chr4: 20712885 .. 20714407 (-)
Sequence length	1097
RNA-Seq Expression	CSPI04G22410.2
Synteny	CSPI04G22410.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTTCTCATCCACCCAATCAAATGCCCCTTCAGCGTTCAATCCAACCACCCAGACTCCATCAATCATCTAAAACACTCCATCATGCGTCTGAGATTGCAGATTGTTCATTTCTTAATCAATTTTCCTCACTACATGAGCCTTACCCTCCCCCTCGGGCCTCCGCTGGTGAGTACTTGAATCAAAACTAAAAGCCCAAGTTCCTTCCTCCTTTTAATATGTCGACAATGGCTGCCAGAAGTTTGAATTTATCTTTTCTTCCAACTGGGTTTATAAGTACTTCAAAAACCCAACAAAGTTTCAACTTTGTTTCTTTTTCTTCTCCTCCAAGTTTCAGAGCAATCAAAAACAAAAAGCTTTCATCATCTTCTACCAGAGTAATGGGCTGCTCTTCCTCCTCTTCCCAGAAACCAGATGGCATCAAAGGTTTTTCCTTTTCCTTTATGAACTTGAGATTTACTAAATATTGATCTATGATATAATGAATTCAAGTAATGATTATCAATGGCGTGGCATGAGAAAATTTACATAGACAGAGAATTTCTCCTACCAATTTGATGCTGTGCATGACTTTGATTTTGAACATTGAAGAAGCTTAGTTGCCTGTTGGTGCAGAGGCTGGCACCGTAAATTACAAATCTTTAAGCGATGATGAATGGAAGAAGCGGCTCACGGGAGAGCAGTTTTATATTACGCGTCAGAAGGGAACTGAGAGAGCTTTTACTGGGTATACTTCAATGAATATCTTCAATATAACGCACCTATTGATCACAGTTCTGATTCCTTTTTAACTTTTTGCTTCAATTTCAGTGAGTATTGGAACACCAAAACGGCAGGGATATACCACTGTATATGTTGTGACACACCTCTGTTTGAGTAAATATTTTGACTCTTTTCGTAACTTCTGTCTGCTACTGTTATTTCATAGAATTTCCAATAATGGTGTGAATATTATCTCTTTCAGATCATCTACTAAATTTGACAGTGGAACTGGTTGGCCATCATATTACCAACCTGTTGGTGATAATGTCAAATCCAAGCTAGACCTTTCCATCATTTTCATGCCTAGAGAGGAAGTTCTTTGTGCTGTCTGTGATGCTCATCTGGGCCATGTATTTAACGATGGACCGCCGCCAACTGGGAAACGTTACTGCATCAATAGGTAAGTTGATATTGCTGGCGTGCTTGTGTGCTTAACTACCTGCTGGTTGTCAGAACTGACTTGTAAATGAAACTTTCTGCAGTGCGGCACTGAAATTGAAGCCAGATAAGTGATGGTGAAGATATTAACATACCTGCCTGCGAATTCTGTAACTGAAACCCGTAATCATTACATGTTTGTGCTTATAAACTGAGGACTCTCGAAAGATGTACACGTTTATTGGGCTGAATGAAAATAATTTCATTGGGTTGTACCTCCCGTGTCTTTGTTCAACATGATAGACGACCCCATCTTGAGTAATTGTTCCAGATATAATGCAGCTAGGCAGAACACAAGCTGAAGCAAGTATCTGGACAAATTGAC

mRNA sequence

ACTTCTCATCCACCCAATCAAATGCCCCTTCAGCGTTCAATCCAACCACCCAGACTCCATCAATCATCTAAAACACTCCATCATGCGTCTGAGATTGCAGATTGTTCATTTCTTAATCAATTTTCCTCACTACATGAGCCTTACCCTCCCCCTCGGGCCTCCGCTGGTGAGTACTTGAATCAAAACTAAAAGCCCAAGTTCCTTCCTCCTTTTAATATGTCGACAATGGCTGCCAGAAGTTTGAATTTATCTTTTCTTCCAACTGGGTTTATAAGTACTTCAAAAACCCAACAAAGTTTCAACTTTGTTTCTTTTTCTTCTCCTCCAAGTTTCAGAGCAATCAAAAACAAAAAGCTTTCATCATCTTCTACCAGAGTAATGGGCTGCTCTTCCTCCTCTTCCCAGAAACCAGATGGCATCAAAGTTGCCTGTTGGTGCAGAGGCTGGCACCGTAAATTACAAATCTTTAAGCGATGATGAATGGAAGAAGCGGCTCACGGGAGAGCAGTTTTATATTACGCGTCAGAAGGGAACTGAGAGAGCTTTTACTGGTGAGTATTGGAACACCAAAACGGCAGGGATATACCACTGTATATGTTGTGACACACCTCTGTTTGAATCATCTACTAAATTTGACAGTGGAACTGGTTGGCCATCATATTACCAACCTGTTGGTGATAATGTCAAATCCAAGCTAGACCTTTCCATCATTTTCATGCCTAGAGAGGAAGTTCTTTGTGCTGTCTGTGATGCTCATCTGGGCCATGTATTTAACGATGGACCGCCGCCAACTGGGAAACGTTACTGCATCAATAGTGCGGCACTGAAATTGAAGCCAGATAAGTGATGGTGAAGATATTAACATACCTGCCTGCGAATTCTGTAACTGAAACCCGTAATCATTACATGTTTGTGCTTATAAACTGAGGACTCTCGAAAGATGTACACGTTTATTGGGCTGAATGAAAATAATTTCATTGGGTTGTACCTCCCGTGTCTTTGTTCAACATGATAGACGACCCCATCTTGAGTAATTGTTCCAGATATAATGCAGCTAGGCAGAACACAAGCTGAAGCAAGTATCTGGACAAATTGAC

Coding sequence (CDS)

ATGGCATCAAAGTTGCCTGTTGGTGCAGAGGCTGGCACCGTAAATTACAAATCTTTAAGCGATGATGAATGGAAGAAGCGGCTCACGGGAGAGCAGTTTTATATTACGCGTCAGAAGGGAACTGAGAGAGCTTTTACTGGTGAGTATTGGAACACCAAAACGGCAGGGATATACCACTGTATATGTTGTGACACACCTCTGTTTGAATCATCTACTAAATTTGACAGTGGAACTGGTTGGCCATCATATTACCAACCTGTTGGTGATAATGTCAAATCCAAGCTAGACCTTTCCATCATTTTCATGCCTAGAGAGGAAGTTCTTTGTGCTGTCTGTGATGCTCATCTGGGCCATGTATTTAACGATGGACCGCCGCCAACTGGGAAACGTTACTGCATCAATAGTGCGGCACTGAAATTGAAGCCAGATAAGTGA

Protein sequence

MASKLPVGAEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDGPPPTGKRYCINSAALKLKPDK*

Homology

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BLAST of CSPI04G22410.2 vs. ExPASy Swiss-Prot
Match: Q0DC89 (Peptide methionine sulfoxide reductase B1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MSRB1 PE=2 SV=2)

HSP 1 Score: 254.6 bits (649), Expect = 6.7e-67
Identity = 112/131 (85.50%), Postives = 122/131 (93.13%), Query Frame = 0


Query: 12  GTVNYK-SLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFES 71
           G  NY  SL+D+EW+KRLT +Q+YITRQKGTERAFTGEYWNTKT GIYHC+CCDTPLFES
Sbjct: 81  GKPNYSTSLTDEEWRKRLTKDQYYITRQKGTERAFTGEYWNTKTPGIYHCVCCDTPLFES 140

Query: 72  STKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDGPPPTGKR 131
           STKFDSGTGWPSYYQP+GDNVK KLD+SIIFMPR EVLCAVCDAHLGHVF+DGP PTGKR
Sbjct: 141 STKFDSGTGWPSYYQPIGDNVKCKLDMSIIFMPRTEVLCAVCDAHLGHVFDDGPRPTGKR 200

Query: 132 YCINSAALKLK 142
           YCINSA+LKLK
Sbjct: 201 YCINSASLKLK 211

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BLAST of CSPI04G22410.2 vs. ExPASy Swiss-Prot
Match: Q9C8M2 (Peptide methionine sulfoxide reductase B1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSRB1 PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 1.4e-64
Identity = 106/131 (80.92%), Postives = 123/131 (93.89%), Query Frame = 0


Query: 10  EAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFE 69
           EA    + SLS++EWKKRLT EQ+YITRQKGTERAFTGEYWN+KT G+Y+C+CCDTPLF+
Sbjct: 64  EAEKNEFASLSENEWKKRLTPEQYYITRQKGTERAFTGEYWNSKTPGVYNCVCCDTPLFD 123

Query: 70  SSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDGPPPTGK 129
           SSTKFDSGTGWPSYYQP+G+NVK+KLDLSIIFMPR+EV+CAVC+AHLGHVF+DGP PTGK
Sbjct: 124 SSTKFDSGTGWPSYYQPIGNNVKTKLDLSIIFMPRQEVVCAVCNAHLGHVFDDGPRPTGK 183

Query: 130 RYCINSAALKL 141
           RYC+NSAALKL
Sbjct: 184 RYCLNSAALKL 194

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BLAST of CSPI04G22410.2 vs. ExPASy Swiss-Prot
Match: Q8DJK9 (Peptide methionine sulfoxide reductase MsrB OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=msrB PE=3 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 5.9e-47
Identity = 83/125 (66.40%), Postives = 100/125 (80.00%), Query Frame = 0


Query: 20  SDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFESSTKFDSGTG 79
           +D EW+ +LT EQ+Y+TR+KGTERAFTG YWN K  G+Y C+CC TPLF S TK+DSGTG
Sbjct: 7   TDAEWQAQLTPEQYYVTRKKGTERAFTGCYWNNKKPGLYSCVCCGTPLFRSETKYDSGTG 66

Query: 80  WPSYYQPVG-DNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDGPPPTGKRYCINSAAL 139
           WPS++QP+  +N++ + DLS   M R EVLCAVCDAHLGHVF DGPPPTG RYCINSAAL
Sbjct: 67  WPSFWQPLDPNNIRMERDLS-HGMVRTEVLCAVCDAHLGHVFEDGPPPTGLRYCINSAAL 126

Query: 140 KLKPD 144
              P+
Sbjct: 127 AFVPE 130

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BLAST of CSPI04G22410.2 vs. ExPASy Swiss-Prot
Match: B0BYW4 (Peptide methionine sulfoxide reductase MsrB OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=msrB PE=3 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.4e-43
Identity = 81/124 (65.32%), Postives = 94/124 (75.81%), Query Frame = 0


Query: 20  SDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFESSTKFDSGTG 79
           ++ EW+ +LT EQF +TR  GTERAFTGEY + K AG Y C+CC T LF S TKFDSGTG
Sbjct: 7   TEQEWEAQLTPEQFRVTRHHGTERAFTGEYHDLKAAGTYQCVCCGTELFTSDTKFDSGTG 66

Query: 80  WPSYYQPVG-DNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDGPPPTGKRYCINSAAL 139
           WPS++ P    +V+ K D S +FM R EVLCAVCDAHLGHVFNDGP PTG RYC+NSAAL
Sbjct: 67  WPSFWAPADKTHVEEKTDRS-LFMVRTEVLCAVCDAHLGHVFNDGPKPTGLRYCMNSAAL 126

Query: 140 KLKP 143
           K  P
Sbjct: 127 KFVP 129

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BLAST of CSPI04G22410.2 vs. ExPASy Swiss-Prot
Match: Q78J03 (Methionine-R-sulfoxide reductase B2, mitochondrial OS=Mus musculus OX=10090 GN=Msrb2 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.3e-41
Identity = 76/140 (54.29%), Postives = 98/140 (70.00%), Query Frame = 0


Query: 13  TVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFESST 72
           T + +SLS+ +W+K+LT EQFY+TR+KGTE  F+G Y N K  G+YHC+CCD+PLF S  
Sbjct: 36  TKSKRSLSEADWQKKLTPEQFYVTREKGTEAPFSGMYLNNKETGMYHCVCCDSPLFSSEK 95

Query: 73  KFDSGTGWPSYYQPVGD--------NVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDGP 132
           K+ SGTGWPS+ +  G          +  +LD S +  PR EV+C  C+AHLGHVF DGP
Sbjct: 96  KYCSGTGWPSFSEAYGSKGSDESHTGILRRLDTS-LGCPRMEVVCKQCEAHLGHVFPDGP 155

Query: 133 PPTGKRYCINSAALKLKPDK 145
            PTG+R+CINS ALK KP K
Sbjct: 156 KPTGQRFCINSVALKFKPSK 174

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BLAST of CSPI04G22410.2 vs. ExPASy TrEMBL
Match: A0A0A0L4I4 (Peptide-methionine (R)-S-oxide reductase OS=Cucumis sativus OX=3659 GN=Csa_4G622800 PE=3 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 5.2e-78
Identity = 138/144 (95.83%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 2   ASKLPVG-AEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 61
           +S+ P G  EAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC
Sbjct: 61  SSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 120

Query: 62  ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 121
           ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF
Sbjct: 121 ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 180

Query: 122 NDGPPPTGKRYCINSAALKLKPDK 145
           NDGPPPTGKRYCINSAALKLKPDK
Sbjct: 181 NDGPPPTGKRYCINSAALKLKPDK 204

[Close]

BLAST of CSPI04G22410.2 vs. ExPASy TrEMBL
Match: A0A5D3D9X6 (Peptide-methionine (R)-S-oxide reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G004200 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.7e-76
Identity = 134/144 (93.06%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 2   ASKLPVG-AEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 61
           +S+ P G  EAGTV+YKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAG+YHC
Sbjct: 6   SSQKPDGIKEAGTVDYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGLYHC 65

Query: 62  ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 121
           ICCDTPLFESSTKFDSGTGWPSYYQP+GDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF
Sbjct: 66  ICCDTPLFESSTKFDSGTGWPSYYQPIGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 125

Query: 122 NDGPPPTGKRYCINSAALKLKPDK 145
           +DGPPPTGKRYCINSAALKLKPDK
Sbjct: 126 DDGPPPTGKRYCINSAALKLKPDK 149

[Close]

BLAST of CSPI04G22410.2 vs. ExPASy TrEMBL
Match: A0A1S3BUL6 (Peptide-methionine (R)-S-oxide reductase OS=Cucumis melo OX=3656 GN=LOC103493463 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.7e-76
Identity = 134/144 (93.06%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 2   ASKLPVG-AEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 61
           +S+ P G  EAGTV+YKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAG+YHC
Sbjct: 60  SSQKPDGIKEAGTVDYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGLYHC 119

Query: 62  ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 121
           ICCDTPLFESSTKFDSGTGWPSYYQP+GDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF
Sbjct: 120 ICCDTPLFESSTKFDSGTGWPSYYQPIGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 179

Query: 122 NDGPPPTGKRYCINSAALKLKPDK 145
           +DGPPPTGKRYCINSAALKLKPDK
Sbjct: 180 DDGPPPTGKRYCINSAALKLKPDK 203

[Close]

BLAST of CSPI04G22410.2 vs. ExPASy TrEMBL
Match: A0A5A7VD42 (Peptide-methionine (R)-S-oxide reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00050 PE=3 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 5.5e-72
Identity = 134/172 (77.91%), Postives = 140/172 (81.40%), Query Frame = 0


Query: 2   ASKLPVG-AEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTG------------- 61
           +S+ P G  EAGTV+YKSLSDDEWKKRLTGEQFYITRQKGTERAFTG             
Sbjct: 6   SSQKPDGIKEAGTVDYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGYASMNIFNIKHLC 65

Query: 62  ---------------EYWNTKTAGIYHCICCDTPLFESSTKFDSGTGWPSYYQPVGDNVK 121
                          EYWNTKTAG+YHCICCDTPLFESSTKFDSGTGWPSYYQP+GDNVK
Sbjct: 66  GRSSDPLLTFFFNFSEYWNTKTAGLYHCICCDTPLFESSTKFDSGTGWPSYYQPIGDNVK 125

Query: 122 SKLDLSIIFMPREEVLCAVCDAHLGHVFNDGPPPTGKRYCINSAALKLKPDK 145
           SKLDLSIIFMPREEVLCAVCDAHLGHVF+DGPPPTGKRYCINSAALKLKPDK
Sbjct: 126 SKLDLSIIFMPREEVLCAVCDAHLGHVFDDGPPPTGKRYCINSAALKLKPDK 177

[Close]

BLAST of CSPI04G22410.2 vs. ExPASy TrEMBL
Match: A0A6J1FBY9 (Peptide-methionine (R)-S-oxide reductase OS=Cucurbita moschata OX=3662 GN=LOC111442706 PE=3 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 8.0e-71
Identity = 125/142 (88.03%), Postives = 132/142 (92.96%), Query Frame = 0


Query: 2   ASKLPVG-AEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 61
           +S+ P G  EAGT +YKSLSDDEWKKRLT EQFYITRQKGTERAFTGEYWNTKT G Y C
Sbjct: 56  SSQRPDGIREAGTADYKSLSDDEWKKRLTSEQFYITRQKGTERAFTGEYWNTKTPGTYQC 115

Query: 62  ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 121
           ICC+TPLFESSTKFDSGTGWPSYYQP+GDNVK+KLDLSIIFMPREEVLCA CDAHLGHVF
Sbjct: 116 ICCNTPLFESSTKFDSGTGWPSYYQPIGDNVKTKLDLSIIFMPREEVLCAACDAHLGHVF 175

Query: 122 NDGPPPTGKRYCINSAALKLKP 143
           +DGPPPTGKRYCINSAALKLKP
Sbjct: 176 DDGPPPTGKRYCINSAALKLKP 197

[Close]

BLAST of CSPI04G22410.2 vs. NCBI nr
Match: XP_004146010.2 (peptide methionine sulfoxide reductase B1, chloroplastic [Cucumis sativus] >KGN55021.1 hypothetical protein Csa_012810 [Cucumis sativus])

HSP 1 Score: 300.1 bits (767), Expect = 1.1e-77
Identity = 138/144 (95.83%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 2   ASKLPVG-AEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 61
           +S+ P G  EAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC
Sbjct: 61  SSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 120

Query: 62  ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 121
           ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF
Sbjct: 121 ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 180

Query: 122 NDGPPPTGKRYCINSAALKLKPDK 145
           NDGPPPTGKRYCINSAALKLKPDK
Sbjct: 181 NDGPPPTGKRYCINSAALKLKPDK 204

[Close]

BLAST of CSPI04G22410.2 vs. NCBI nr
Match: XP_038898315.1 (peptide methionine sulfoxide reductase B1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 298.1 bits (762), Expect = 4.0e-77
Identity = 134/140 (95.71%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 3   SKLPVGAEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCIC 62
           ++LPVGAEAGTV+YKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKT GIYHCIC
Sbjct: 70  AQLPVGAEAGTVDYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTPGIYHCIC 129

Query: 63  CDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFND 122
           C+TPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF+D
Sbjct: 130 CNTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFDD 189

Query: 123 GPPPTGKRYCINSAALKLKP 143
           GPPPTGKRYCINSAALKLKP
Sbjct: 190 GPPPTGKRYCINSAALKLKP 209

[Close]

BLAST of CSPI04G22410.2 vs. NCBI nr
Match: TYK20280.1 (peptide methionine sulfoxide reductase B1 [Cucumis melo var. makuwa])

HSP 1 Score: 295.0 bits (754), Expect = 3.4e-76
Identity = 134/144 (93.06%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 2   ASKLPVG-AEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 61
           +S+ P G  EAGTV+YKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAG+YHC
Sbjct: 6   SSQKPDGIKEAGTVDYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGLYHC 65

Query: 62  ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 121
           ICCDTPLFESSTKFDSGTGWPSYYQP+GDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF
Sbjct: 66  ICCDTPLFESSTKFDSGTGWPSYYQPIGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 125

Query: 122 NDGPPPTGKRYCINSAALKLKPDK 145
           +DGPPPTGKRYCINSAALKLKPDK
Sbjct: 126 DDGPPPTGKRYCINSAALKLKPDK 149

[Close]

BLAST of CSPI04G22410.2 vs. NCBI nr
Match: XP_008452427.1 (PREDICTED: peptide methionine sulfoxide reductase B1, chloroplastic [Cucumis melo])

HSP 1 Score: 295.0 bits (754), Expect = 3.4e-76
Identity = 134/144 (93.06%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 2   ASKLPVG-AEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 61
           +S+ P G  EAGTV+YKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAG+YHC
Sbjct: 60  SSQKPDGIKEAGTVDYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGLYHC 119

Query: 62  ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 121
           ICCDTPLFESSTKFDSGTGWPSYYQP+GDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF
Sbjct: 120 ICCDTPLFESSTKFDSGTGWPSYYQPIGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 179

Query: 122 NDGPPPTGKRYCINSAALKLKPDK 145
           +DGPPPTGKRYCINSAALKLKPDK
Sbjct: 180 DDGPPPTGKRYCINSAALKLKPDK 203

[Close]

BLAST of CSPI04G22410.2 vs. NCBI nr
Match: XP_038898316.1 (peptide methionine sulfoxide reductase B1, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 288.1 bits (736), Expect = 4.2e-74
Identity = 132/142 (92.96%), Postives = 137/142 (96.48%), Query Frame = 0


Query: 2   ASKLPVG-AEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 61
           +S+ P G  EAGTV+YKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKT GIYHC
Sbjct: 60  SSQRPDGIQEAGTVDYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTPGIYHC 119

Query: 62  ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 121
           ICC+TPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF
Sbjct: 120 ICCNTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 179

Query: 122 NDGPPPTGKRYCINSAALKLKP 143
           +DGPPPTGKRYCINSAALKLKP
Sbjct: 180 DDGPPPTGKRYCINSAALKLKP 201

[Close]

BLAST of CSPI04G22410.2 vs. TAIR 10
Match: AT1G53670.1 (methionine sulfoxide reductase B 1 )

HSP 1 Score: 246.9 bits (629), Expect = 1.0e-65
Identity = 106/131 (80.92%), Postives = 123/131 (93.89%), Query Frame = 0


Query: 10  EAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFE 69
           EA    + SLS++EWKKRLT EQ+YITRQKGTERAFTGEYWN+KT G+Y+C+CCDTPLF+
Sbjct: 64  EAEKNEFASLSENEWKKRLTPEQYYITRQKGTERAFTGEYWNSKTPGVYNCVCCDTPLFD 123

Query: 70  SSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDGPPPTGK 129
           SSTKFDSGTGWPSYYQP+G+NVK+KLDLSIIFMPR+EV+CAVC+AHLGHVF+DGP PTGK
Sbjct: 124 SSTKFDSGTGWPSYYQPIGNNVKTKLDLSIIFMPRQEVVCAVCNAHLGHVFDDGPRPTGK 183

Query: 130 RYCINSAALKL 141
           RYC+NSAALKL
Sbjct: 184 RYCLNSAALKL 194

[Close]

BLAST of CSPI04G22410.2 vs. TAIR 10
Match: AT1G53670.2 (methionine sulfoxide reductase B 1 )

HSP 1 Score: 226.5 bits (576), Expect = 1.4e-59
Identity = 99/131 (75.57%), Postives = 116/131 (88.55%), Query Frame = 0


Query: 10  EAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFE 69
           EA    + SLS++EWKKRLT EQ+YITRQKGTERAFTGEYWN+KT G+Y+C+CCDTPLF+
Sbjct: 64  EAEKNEFASLSENEWKKRLTPEQYYITRQKGTERAFTGEYWNSKTPGVYNCVCCDTPLFD 123

Query: 70  SSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDGPPPTGK 129
                  GTGWPSYYQP+G+NVK+KLDLSIIFMPR+EV+CAVC+AHLGHVF+DGP PTGK
Sbjct: 124 -------GTGWPSYYQPIGNNVKTKLDLSIIFMPRQEVVCAVCNAHLGHVFDDGPRPTGK 183

Query: 130 RYCINSAALKL 141
           RYC+NSAALKL
Sbjct: 184 RYCLNSAALKL 187

[Close]

BLAST of CSPI04G22410.2 vs. TAIR 10
Match: AT4G04830.1 (methionine sulfoxide reductase B5 )

HSP 1 Score: 119.4 bits (298), Expect = 2.4e-27
Identity = 56/127 (44.09%), Postives = 80/127 (62.99%), Query Frame = 0


Query: 20  SDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFESSTKFDSGTG 79
           +++EW+  L+ EQF I RQKGTE+  TGEY      GI+ C+ C TPL++S+TKFDSG G
Sbjct: 11  TEEEWRAVLSPEQFRILRQKGTEKPGTGEYDKFFEEGIFDCVGCKTPLYKSTTKFDSGCG 70

Query: 80  WPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF-NDG-PPPTGKRYCINSAA 139
           WP++++ +   +    D       R E+ CA CD HLGHVF  +G   PT +R+C+NS +
Sbjct: 71  WPAFFEGLPGAINRTPDPD---GRRTEITCAACDGHLGHVFKGEGYGNPTDERHCVNSVS 130

Query: 140 LKLKPDK 145
           +   P K
Sbjct: 131 ISFNPAK 134

[Close]

BLAST of CSPI04G22410.2 vs. TAIR 10
Match: AT4G21860.1 (methionine sulfoxide reductase B 2 )

HSP 1 Score: 117.9 bits (294), Expect = 7.0e-27
Identity = 58/125 (46.40%), Postives = 77/125 (61.60%), Query Frame = 0


Query: 21  DDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFESSTKFDSGTGW 80
           ++EW+  L+ EQF I RQKGTE   TGEY      GIY C  C TPL++S+TKFDSG GW
Sbjct: 77  EEEWRAILSPEQFRILRQKGTEYPGTGEYNKVFDDGIYCCAGCGTPLYKSTTKFDSGCGW 136

Query: 81  PSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF-NDG-PPPTGKRYCINSAAL 140
           P+++  +   +    D       R E+ CA C  HLGHVF  +G P PT +R+C+NS +L
Sbjct: 137 PAFFDGLPGAITRTPDPD---GRRIEITCAACGGHLGHVFKGEGFPTPTDERHCVNSISL 196

Query: 141 KLKPD 144
           K  P+
Sbjct: 197 KFTPE 198

[Close]

BLAST of CSPI04G22410.2 vs. TAIR 10
Match: AT4G21860.3 (methionine sulfoxide reductase B 2 )

HSP 1 Score: 117.9 bits (294), Expect = 7.0e-27
Identity = 58/125 (46.40%), Postives = 77/125 (61.60%), Query Frame = 0


Query: 21  DDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFESSTKFDSGTGW 80
           ++EW+  L+ EQF I RQKGTE   TGEY      GIY C  C TPL++S+TKFDSG GW
Sbjct: 77  EEEWRAILSPEQFRILRQKGTEYPGTGEYNKVFDDGIYCCAGCGTPLYKSTTKFDSGCGW 136

Query: 81  PSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF-NDG-PPPTGKRYCINSAAL 140
           P+++  +   +    D       R E+ CA C  HLGHVF  +G P PT +R+C+NS +L
Sbjct: 137 PAFFDGLPGAITRTPDPD---GRRIEITCAACGGHLGHVFKGEGFPTPTDERHCVNSISL 196

Query: 141 KLKPD 144
           K  P+
Sbjct: 197 KFTPE 198

The following BLAST results are available for this feature:

BLAST of CSPI04G22410.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0DC89	6.7e-67	85.50	Peptide methionine sulfoxide reductase B1, chloroplastic OS=Oryza sativa subsp. ...	[more]
Q9C8M2	1.4e-64	80.92	Peptide methionine sulfoxide reductase B1, chloroplastic OS=Arabidopsis thaliana...	[more]
Q8DJK9	5.9e-47	66.40	Peptide methionine sulfoxide reductase MsrB OS=Thermosynechococcus elongatus (st...	[more]
B0BYW4	1.4e-43	65.32	Peptide methionine sulfoxide reductase MsrB OS=Acaryochloris marina (strain MBIC...	[more]
Q78J03	1.3e-41	54.29	Methionine-R-sulfoxide reductase B2, mitochondrial OS=Mus musculus OX=10090 GN=M...	[more]

BLAST of CSPI04G22410.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L4I4	5.2e-78	95.83	Peptide-methionine (R)-S-oxide reductase OS=Cucumis sativus OX=3659 GN=Csa_4G622...	[more]
A0A5D3D9X6	1.7e-76	93.06	Peptide-methionine (R)-S-oxide reductase OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A1S3BUL6	1.7e-76	93.06	Peptide-methionine (R)-S-oxide reductase OS=Cucumis melo OX=3656 GN=LOC103493463...	[more]
A0A5A7VD42	5.5e-72	77.91	Peptide-methionine (R)-S-oxide reductase OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A6J1FBY9	8.0e-71	88.03	Peptide-methionine (R)-S-oxide reductase OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of CSPI04G22410.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146010.2	1.1e-77	95.83	peptide methionine sulfoxide reductase B1, chloroplastic [Cucumis sativus] >KGN5...	[more]
XP_038898315.1	4.0e-77	95.71	peptide methionine sulfoxide reductase B1, chloroplastic isoform X1 [Benincasa h...	[more]
TYK20280.1	3.4e-76	93.06	peptide methionine sulfoxide reductase B1 [Cucumis melo var. makuwa]	[more]
XP_008452427.1	3.4e-76	93.06	PREDICTED: peptide methionine sulfoxide reductase B1, chloroplastic [Cucumis mel...	[more]
XP_038898316.1	4.2e-74	92.96	peptide methionine sulfoxide reductase B1, chloroplastic isoform X2 [Benincasa h...	[more]

BLAST of CSPI04G22410.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G53670.1	1.0e-65	80.92	methionine sulfoxide reductase B 1	[more]
AT1G53670.2	1.4e-59	75.57	methionine sulfoxide reductase B 1	[more]
AT4G04830.1	2.4e-27	44.09	methionine sulfoxide reductase B5	[more]
AT4G21860.1	7.0e-27	46.40	methionine sulfoxide reductase B 2	[more]
AT4G21860.3	7.0e-27	46.40	methionine sulfoxide reductase B 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 144..144
None	No IPR available	GENE3D	2.170.150.20	Peptide methionine sulfoxide reductase.	coord: 11..144 e-value: 1.9E-59 score: 201.5
None	No IPR available	PANTHER	PTHR10173:SF52	METHIONINE-R-SULFOXIDE REDUCTASE B1	coord: 13..143
IPR002579	Peptide methionine sulphoxide reductase MrsB	PFAM	PF01641	SelR	coord: 23..142 e-value: 3.6E-52 score: 175.5
IPR002579	Peptide methionine sulphoxide reductase MrsB	TIGRFAM	TIGR00357	TIGR00357	coord: 19..142 e-value: 9.4E-56 score: 185.5
IPR002579	Peptide methionine sulphoxide reductase MrsB	PROSITE	PS51790	MSRB	coord: 21..143 score: 73.200645
IPR028427	Peptide methionine sulfoxide reductase	PANTHER	PTHR10173	METHIONINE SULFOXIDE REDUCTASE	coord: 13..143
IPR011057	Mss4-like superfamily	SUPERFAMILY	51316	Mss4-like	coord: 19..143

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI04G22410	CSPI04G22410	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G22410.2.utr3p1	CSPI04G22410.2.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G22410.2.cds5	CSPI04G22410.2.cds5	CDS
CSPI04G22410.2.cds4	CSPI04G22410.2.cds4	CDS
CSPI04G22410.2.cds3	CSPI04G22410.2.cds3	CDS
CSPI04G22410.2.cds2	CSPI04G22410.2.cds2	CDS
CSPI04G22410.2.cds1	CSPI04G22410.2.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G22410.2.utr5p1	CSPI04G22410.2.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI04G22410.2	CSPI04G22410.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030091	protein repair
biological_process	GO:0006979	response to oxidative stress
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0016671	oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor
molecular_function	GO:0033743	peptide-methionine (R)-S-oxide reductase activity
molecular_function	GO:0046872	metal ion binding