CSPI04G22410 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G22410
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Peptide-methionine (R)-S-oxide reductase
Location	Chr4: 20712885 .. 20714407 (-)
RNA-Seq Expression	CSPI04G22410
Synteny	CSPI04G22410

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTTCTCATCCACCCAATCAAATGCCCCTTCAGCGTTCAATCCAACCACCCAGACTCCATCAATCATCTAAAACACTCCATCATGCGTCTGAGATTGCAGATTGTTCATTTCTTAATCAATTTTCCTCACTACATGAGCCTTACCCTCCCCCTCGGGCCTCCGCTGGTGAGTACTTGAATCAAAACTAAAAGCCCAAGTTCCTTCCTCCTTTTAATATGTCGACAATGGCTGCCAGAAGTTTGAATTTATCTTTTCTTCCAACTGGGTTTATAAGTACTTCAAAAACCCAACAAAGTTTCAACTTTGTTTCTTTTTCTTCTCCTCCAAGTTTCAGAGCAATCAAAAACAAAAAGCTTTCATCATCTTCTACCAGAGTAATGGGCTGCTCTTCCTCCTCTTCCCAGAAACCAGATGGCATCAAAGGTTTTTCCTTTTCCTTTATGAACTTGAGATTTACTAAATATTGATCTATGATATAATGAATTCAAGTAATGATTATCAATGGCGTGGCATGAGAAAATTTACATAGACAGAGAATTTCTCCTACCAATTTGATGCTGTGCATGACTTTGATTTTGAACATTGAAGAAGCTTAGTTGCCTGTTGGTGCAGAGGCTGGCACCGTAAATTACAAATCTTTAAGCGATGATGAATGGAAGAAGCGGCTCACGGGAGAGCAGTTTTATATTACGCGTCAGAAGGGAACTGAGAGAGCTTTTACTGGGTATACTTCAATGAATATCTTCAATATAACGCACCTATTGATCACAGTTCTGATTCCTTTTTAACTTTTTGCTTCAATTTCAGTGAGTATTGGAACACCAAAACGGCAGGGATATACCACTGTATATGTTGTGACACACCTCTGTTTGAGTAAATATTTTGACTCTTTTCGTAACTTCTGTCTGCTACTGTTATTTCATAGAATTTCCAATAATGGTGTGAATATTATCTCTTTCAGATCATCTACTAAATTTGACAGTGGAACTGGTTGGCCATCATATTACCAACCTGTTGGTGATAATGTCAAATCCAAGCTAGACCTTTCCATCATTTTCATGCCTAGAGAGGAAGTTCTTTGTGCTGTCTGTGATGCTCATCTGGGCCATGTATTTAACGATGGACCGCCGCCAACTGGGAAACGTTACTGCATCAATAGGTAAGTTGATATTGCTGGCGTGCTTGTGTGCTTAACTACCTGCTGGTTGTCAGAACTGACTTGTAAATGAAACTTTCTGCAGTGCGGCACTGAAATTGAAGCCAGATAAGTGATGGTGAAGATATTAACATACCTGCCTGCGAATTCTGTAACTGAAACCCGTAATCATTACATGTTTGTGCTTATAAACTGAGGACTCTCGAAAGATGTACACGTTTATTGGGCTGAATGAAAATAATTTCATTGGGTTGTACCTCCCGTGTCTTTGTTCAACATGATAGACGACCCCATCTTGAGTAATTGTTCCAGATATAATGCAGCTAGGCAGAACACAAGCTGAAGCAAGTATCTGGACAAATTGAC

mRNA sequence

ACTTCTCATCCACCCAATCAAATGCCCCTTCAGCGTTCAATCCAACCACCCAGACTCCATCAATCATCTAAAACACTCCATCATGCGTCTGAGATTGCAGATTGTTCATTTCTTAATCAATTTTCCTCACTACATGAGCCTTACCCTCCCCCTCGGGCCTCCGCTGGTGAGTACTTGAATCAAAACTAAAAGCCCAAGTTCCTTCCTCCTTTTAATATGTCGACAATGGCTGCCAGAAGTTTGAATTTATCTTTTCTTCCAACTGGGTTTATAAGTACTTCAAAAACCCAACAAAGTTTCAACTTTGTTTCTTTTTCTTCTCCTCCAAGTTTCAGAGCAATCAAAAACAAAAAGCTTTCATCATCTTCTACCAGAGTAATGGGCTGCTCTTCCTCCTCTTCCCAGAAACCAGATGGCATCAAAGAGGCTGGCACCGTAAATTACAAATCTTTAAGCGATGATGAATGGAAGAAGCGGCTCACGGGAGAGCAGTTTTATATTACGCGTCAGAAGGGAACTGAGAGAGCTTTTACTGGTGAGTATTGGAACACCAAAACGGCAGGGATATACCACTGTATATGTTGTGACACACCTCTGTTTGAATCATCTACTAAATTTGACAGTGGAACTGGTTGGCCATCATATTACCAACCTGTTGGTGATAATGTCAAATCCAAGCTAGACCTTTCCATCATTTTCATGCCTAGAGAGGAAGTTCTTTGTGCTGTCTGTGATGCTCATCTGGGCCATGTATTTAACGATGGACCGCCGCCAACTGGGAAACGTTACTGCATCAATAGTGCGGCACTGAAATTGAAGCCAGATAAGTGATGGTGAAGATATTAACATACCTGCCTGCGAATTCTGTAACTGAAACCCGTAATCATTACATGTTTGTGCTTATAAACTGAGGACTCTCGAAAGATGTACACGTTTATTGGGCTGAATGAAAATAATTTCATTGGGTTGTACCTCCCGTGTCTTTGTTCAACATGATAGACGACCCCATCTTGAGTAATTGTTCCAGATATAATGCAGCTAGGCAGAACACAAGCTGAAGCAAGTATCTGGACAAATTGAC

Coding sequence (CDS)

ATGTCGACAATGGCTGCCAGAAGTTTGAATTTATCTTTTCTTCCAACTGGGTTTATAAGTACTTCAAAAACCCAACAAAGTTTCAACTTTGTTTCTTTTTCTTCTCCTCCAAGTTTCAGAGCAATCAAAAACAAAAAGCTTTCATCATCTTCTACCAGAGTAATGGGCTGCTCTTCCTCCTCTTCCCAGAAACCAGATGGCATCAAAGAGGCTGGCACCGTAAATTACAAATCTTTAAGCGATGATGAATGGAAGAAGCGGCTCACGGGAGAGCAGTTTTATATTACGCGTCAGAAGGGAACTGAGAGAGCTTTTACTGGTGAGTATTGGAACACCAAAACGGCAGGGATATACCACTGTATATGTTGTGACACACCTCTGTTTGAATCATCTACTAAATTTGACAGTGGAACTGGTTGGCCATCATATTACCAACCTGTTGGTGATAATGTCAAATCCAAGCTAGACCTTTCCATCATTTTCATGCCTAGAGAGGAAGTTCTTTGTGCTGTCTGTGATGCTCATCTGGGCCATGTATTTAACGATGGACCGCCGCCAACTGGGAAACGTTACTGCATCAATAGTGCGGCACTGAAATTGAAGCCAGATAAGTGA

Protein sequence

MSTMAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSSSSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDGPPPTGKRYCINSAALKLKPDK*

Homology

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BLAST of CSPI04G22410 vs. ExPASy Swiss-Prot
Match: Q9C8M2 (Peptide methionine sulfoxide reductase B1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSRB1 PE=1 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 2.0e-69
Identity = 128/197 (64.97%), Postives = 158/197 (80.20%), Query Frame = 0


Query: 6   ARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSF--RAIKNKKLSSSSTRVMGCSSSSSQ 65
           A S  L+ + + F+S    +   N+VS ++   F  R++   +  S S   MG SSSSS 
Sbjct: 2   ASSTRLTIIQSSFVS---ARTRLNYVSKTNHSGFACRSLSKPRNLSLSVYSMG-SSSSSP 61

Query: 66  KPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICC 125
           KPD ++EA    + SLS++EWKKRLT EQ+YITRQKGTERAFTGEYWN+KT G+Y+C+CC
Sbjct: 62  KPDNVQEAEKNEFASLSENEWKKRLTPEQYYITRQKGTERAFTGEYWNSKTPGVYNCVCC 121

Query: 126 DTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDG 185
           DTPLF+SSTKFDSGTGWPSYYQP+G+NVK+KLDLSIIFMPR+EV+CAVC+AHLGHVF+DG
Sbjct: 122 DTPLFDSSTKFDSGTGWPSYYQPIGNNVKTKLDLSIIFMPRQEVVCAVCNAHLGHVFDDG 181

Query: 186 PPPTGKRYCINSAALKL 201
           P PTGKRYC+NSAALKL
Sbjct: 182 PRPTGKRYCLNSAALKL 194

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BLAST of CSPI04G22410 vs. ExPASy Swiss-Prot
Match: Q0DC89 (Peptide methionine sulfoxide reductase B1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MSRB1 PE=2 SV=2)

HSP 1 Score: 260.0 bits (663), Expect = 2.3e-68
Identity = 124/162 (76.54%), Postives = 136/162 (83.95%), Query Frame = 0


Query: 50  SSTRVMGC------SSSSSQKPDGIKEA---GTVNYK-SLSDDEWKKRLTGEQFYITRQK 109
           +S R MG       SSSSS  P G  +A   G  NY  SL+D+EW+KRLT +Q+YITRQK
Sbjct: 50  ASVRAMGAASSSSSSSSSSPSPQGQAQAQAQGKPNYSTSLTDEEWRKRLTKDQYYITRQK 109

Query: 110 GTERAFTGEYWNTKTAGIYHCICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSI 169
           GTERAFTGEYWNTKT GIYHC+CCDTPLFESSTKFDSGTGWPSYYQP+GDNVK KLD+SI
Sbjct: 110 GTERAFTGEYWNTKTPGIYHCVCCDTPLFESSTKFDSGTGWPSYYQPIGDNVKCKLDMSI 169

Query: 170 IFMPREEVLCAVCDAHLGHVFNDGPPPTGKRYCINSAALKLK 202
           IFMPR EVLCAVCDAHLGHVF+DGP PTGKRYCINSA+LKLK
Sbjct: 170 IFMPRTEVLCAVCDAHLGHVFDDGPRPTGKRYCINSASLKLK 211

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BLAST of CSPI04G22410 vs. ExPASy Swiss-Prot
Match: Q8DJK9 (Peptide methionine sulfoxide reductase MsrB OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=msrB PE=3 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.4e-46
Identity = 83/125 (66.40%), Postives = 100/125 (80.00%), Query Frame = 0


Query: 80  SDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFESSTKFDSGTG 139
           +D EW+ +LT EQ+Y+TR+KGTERAFTG YWN K  G+Y C+CC TPLF S TK+DSGTG
Sbjct: 7   TDAEWQAQLTPEQYYVTRKKGTERAFTGCYWNNKKPGLYSCVCCGTPLFRSETKYDSGTG 66

Query: 140 WPSYYQPVG-DNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDGPPPTGKRYCINSAAL 199
           WPS++QP+  +N++ + DLS   M R EVLCAVCDAHLGHVF DGPPPTG RYCINSAAL
Sbjct: 67  WPSFWQPLDPNNIRMERDLS-HGMVRTEVLCAVCDAHLGHVFEDGPPPTGLRYCINSAAL 126

Query: 200 KLKPD 204
              P+
Sbjct: 127 AFVPE 130

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BLAST of CSPI04G22410 vs. ExPASy Swiss-Prot
Match: B0BYW4 (Peptide methionine sulfoxide reductase MsrB OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=msrB PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.5e-43
Identity = 81/124 (65.32%), Postives = 94/124 (75.81%), Query Frame = 0


Query: 80  SDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFESSTKFDSGTG 139
           ++ EW+ +LT EQF +TR  GTERAFTGEY + K AG Y C+CC T LF S TKFDSGTG
Sbjct: 7   TEQEWEAQLTPEQFRVTRHHGTERAFTGEYHDLKAAGTYQCVCCGTELFTSDTKFDSGTG 66

Query: 140 WPSYYQPVG-DNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDGPPPTGKRYCINSAAL 199
           WPS++ P    +V+ K D S +FM R EVLCAVCDAHLGHVFNDGP PTG RYC+NSAAL
Sbjct: 67  WPSFWAPADKTHVEEKTDRS-LFMVRTEVLCAVCDAHLGHVFNDGPKPTGLRYCMNSAAL 126

Query: 200 KLKP 203
           K  P
Sbjct: 127 KFVP 129

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BLAST of CSPI04G22410 vs. ExPASy Swiss-Prot
Match: Q78J03 (Methionine-R-sulfoxide reductase B2, mitochondrial OS=Mus musculus OX=10090 GN=Msrb2 PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.4e-41
Identity = 84/174 (48.28%), Postives = 113/174 (64.94%), Query Frame = 0


Query: 40  RAIKNKKLSSSSTRV-MGCSSSSSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQ 99
           RA++   L  +  R+  GC+ S S+    +    T + +SLS+ +W+K+LT EQFY+TR+
Sbjct: 6   RALRGLPLLQAPGRLARGCAGSGSKDTGSL----TKSKRSLSEADWQKKLTPEQFYVTRE 65

Query: 100 KGTERAFTGEYWNTKTAGIYHCICCDTPLFESSTKFDSGTGWPSYYQPVGD--------N 159
           KGTE  F+G Y N K  G+YHC+CCD+PLF S  K+ SGTGWPS+ +  G          
Sbjct: 66  KGTEAPFSGMYLNNKETGMYHCVCCDSPLFSSEKKYCSGTGWPSFSEAYGSKGSDESHTG 125

Query: 160 VKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDGPPPTGKRYCINSAALKLKPDK 205
           +  +LD S +  PR EV+C  C+AHLGHVF DGP PTG+R+CINS ALK KP K
Sbjct: 126 ILRRLDTS-LGCPRMEVVCKQCEAHLGHVFPDGPKPTGQRFCINSVALKFKPSK 174

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BLAST of CSPI04G22410 vs. ExPASy TrEMBL
Match: A0A0A0L4I4 (Peptide-methionine (R)-S-oxide reductase OS=Cucumis sativus OX=3659 GN=Csa_4G622800 PE=3 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.2e-114
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 0


Query: 1   MSTMAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSS 60
           MSTMAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSS
Sbjct: 1   MSTMAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSS 60

Query: 61  SSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 120
           SSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC
Sbjct: 61  SSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 120

Query: 121 ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 180
           ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF
Sbjct: 121 ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 180

Query: 181 NDGPPPTGKRYCINSAALKLKPDK 205
           NDGPPPTGKRYCINSAALKLKPDK
Sbjct: 181 NDGPPPTGKRYCINSAALKLKPDK 204

[Close]

BLAST of CSPI04G22410 vs. ExPASy TrEMBL
Match: A0A1S3BUL6 (Peptide-methionine (R)-S-oxide reductase OS=Cucumis melo OX=3656 GN=LOC103493463 PE=3 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 3.0e-108
Identity = 195/204 (95.59%), Postives = 200/204 (98.04%), Query Frame = 0


Query: 1   MSTMAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSS 60
           MSTMAARSLNLS LPTGFISTSKTQQSFNF+SFSSPP FRAIKNKKLSS STRVMG SSS
Sbjct: 1   MSTMAARSLNLSILPTGFISTSKTQQSFNFLSFSSPPGFRAIKNKKLSSFSTRVMG-SSS 60

Query: 61  SSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 120
           SSQKPDGIKEAGTV+YKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAG+YHC
Sbjct: 61  SSQKPDGIKEAGTVDYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGLYHC 120

Query: 121 ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 180
           ICCDTPLFESSTKFDSGTGWPSYYQP+GDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF
Sbjct: 121 ICCDTPLFESSTKFDSGTGWPSYYQPIGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 180

Query: 181 NDGPPPTGKRYCINSAALKLKPDK 205
           +DGPPPTGKRYCINSAALKLKPDK
Sbjct: 181 DDGPPPTGKRYCINSAALKLKPDK 203

[Close]

BLAST of CSPI04G22410 vs. ExPASy TrEMBL
Match: A0A6J1FBY9 (Peptide-methionine (R)-S-oxide reductase OS=Cucurbita moschata OX=3662 GN=LOC111442706 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.4e-91
Identity = 168/199 (84.42%), Postives = 182/199 (91.46%), Query Frame = 0


Query: 4   MAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSSSSQ 63
           MA+RSLNLS +P+GFI++SKTQQ+F FVS  SP  FRA K +KLSSSS R MG SSSSSQ
Sbjct: 1   MASRSLNLS-IPSGFINSSKTQQNFKFVSLPSPLIFRAPKYEKLSSSSIRAMG-SSSSSQ 60

Query: 64  KPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICC 123
           +PDGI+EAGT +YKSLSDDEWKKRLT EQFYITRQKGTERAFTGEYWNTKT G Y CICC
Sbjct: 61  RPDGIREAGTADYKSLSDDEWKKRLTSEQFYITRQKGTERAFTGEYWNTKTPGTYQCICC 120

Query: 124 DTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDG 183
           +TPLFESSTKFDSGTGWPSYYQP+GDNVK+KLDLSIIFMPREEVLCA CDAHLGHVF+DG
Sbjct: 121 NTPLFESSTKFDSGTGWPSYYQPIGDNVKTKLDLSIIFMPREEVLCAACDAHLGHVFDDG 180

Query: 184 PPPTGKRYCINSAALKLKP 203
           PPPTGKRYCINSAALKLKP
Sbjct: 181 PPPTGKRYCINSAALKLKP 197

[Close]

BLAST of CSPI04G22410 vs. ExPASy TrEMBL
Match: A0A6J1BQM8 (Peptide-methionine (R)-S-oxide reductase OS=Momordica charantia OX=3673 GN=LOC111004855 PE=3 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 1.3e-82
Identity = 163/201 (81.09%), Postives = 175/201 (87.06%), Query Frame = 0


Query: 4   MAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSSSSQ 63
           MAARS +LS +PTGF+++SKTQQ F  + F S PSFRA   KKLSSS  R MG SSSSSQ
Sbjct: 1   MAARSSSLS-IPTGFLNSSKTQQGFKII-FVSSPSFRA--PKKLSSSFVRAMG-SSSSSQ 60

Query: 64  KPDGIK--EAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCI 123
           +PDGI+  EA T +YKS+S+DEWKKRLT EQF ITRQKGTERAFTGEYWNTKT G YHCI
Sbjct: 61  RPDGIREAEADTTDYKSISNDEWKKRLTKEQFDITRQKGTERAFTGEYWNTKTPGTYHCI 120

Query: 124 CCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFN 183
           CCDT LFESSTKFDSGTGWPSYYQPV DNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF+
Sbjct: 121 CCDTTLFESSTKFDSGTGWPSYYQPVRDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFD 180

Query: 184 DGPPPTGKRYCINSAALKLKP 203
           DGP PTGKRYCINSAALKLKP
Sbjct: 181 DGPAPTGKRYCINSAALKLKP 196

[Close]

BLAST of CSPI04G22410 vs. ExPASy TrEMBL
Match: A0A5D3D9X6 (Peptide-methionine (R)-S-oxide reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G004200 PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 6.4e-82
Identity = 143/147 (97.28%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 58  SSSSSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGI 117
           SSSSSQKPDGIKEAGTV+YKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAG+
Sbjct: 3   SSSSSQKPDGIKEAGTVDYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGL 62

Query: 118 YHCICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLG 177
           YHCICCDTPLFESSTKFDSGTGWPSYYQP+GDNVKSKLDLSIIFMPREEVLCAVCDAHLG
Sbjct: 63  YHCICCDTPLFESSTKFDSGTGWPSYYQPIGDNVKSKLDLSIIFMPREEVLCAVCDAHLG 122

Query: 178 HVFNDGPPPTGKRYCINSAALKLKPDK 205
           HVF+DGPPPTGKRYCINSAALKLKPDK
Sbjct: 123 HVFDDGPPPTGKRYCINSAALKLKPDK 149

[Close]

BLAST of CSPI04G22410 vs. NCBI nr
Match: XP_004146010.2 (peptide methionine sulfoxide reductase B1, chloroplastic [Cucumis sativus] >KGN55021.1 hypothetical protein Csa_012810 [Cucumis sativus])

HSP 1 Score: 421.4 bits (1082), Expect = 4.5e-114
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 0


Query: 1   MSTMAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSS 60
           MSTMAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSS
Sbjct: 1   MSTMAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSS 60

Query: 61  SSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 120
           SSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC
Sbjct: 61  SSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 120

Query: 121 ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 180
           ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF
Sbjct: 121 ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 180

Query: 181 NDGPPPTGKRYCINSAALKLKPDK 205
           NDGPPPTGKRYCINSAALKLKPDK
Sbjct: 181 NDGPPPTGKRYCINSAALKLKPDK 204

[Close]

BLAST of CSPI04G22410 vs. NCBI nr
Match: XP_008452427.1 (PREDICTED: peptide methionine sulfoxide reductase B1, chloroplastic [Cucumis melo])

HSP 1 Score: 401.0 bits (1029), Expect = 6.3e-108
Identity = 195/204 (95.59%), Postives = 200/204 (98.04%), Query Frame = 0


Query: 1   MSTMAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSS 60
           MSTMAARSLNLS LPTGFISTSKTQQSFNF+SFSSPP FRAIKNKKLSS STRVMG SSS
Sbjct: 1   MSTMAARSLNLSILPTGFISTSKTQQSFNFLSFSSPPGFRAIKNKKLSSFSTRVMG-SSS 60

Query: 61  SSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 120
           SSQKPDGIKEAGTV+YKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAG+YHC
Sbjct: 61  SSQKPDGIKEAGTVDYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGLYHC 120

Query: 121 ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 180
           ICCDTPLFESSTKFDSGTGWPSYYQP+GDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF
Sbjct: 121 ICCDTPLFESSTKFDSGTGWPSYYQPIGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 180

Query: 181 NDGPPPTGKRYCINSAALKLKPDK 205
           +DGPPPTGKRYCINSAALKLKPDK
Sbjct: 181 DDGPPPTGKRYCINSAALKLKPDK 203

[Close]

BLAST of CSPI04G22410 vs. NCBI nr
Match: XP_038898316.1 (peptide methionine sulfoxide reductase B1, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 375.2 bits (962), Expect = 3.7e-100
Identity = 183/202 (90.59%), Postives = 192/202 (95.05%), Query Frame = 0


Query: 1   MSTMAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSS 60
           MS MAARSLN S LPTGFIS+SKTQQ+F F+S SSPPSF + KNKKLSSSS RVMG SSS
Sbjct: 1   MSAMAARSLNSSILPTGFISSSKTQQNFKFLSLSSPPSFGSPKNKKLSSSSVRVMG-SSS 60

Query: 61  SSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHC 120
           SSQ+PDGI+EAGTV+YKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKT GIYHC
Sbjct: 61  SSQRPDGIQEAGTVDYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTPGIYHC 120

Query: 121 ICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 180
           ICC+TPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF
Sbjct: 121 ICCNTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF 180

Query: 181 NDGPPPTGKRYCINSAALKLKP 203
           +DGPPPTGKRYCINSAALKLKP
Sbjct: 181 DDGPPPTGKRYCINSAALKLKP 201

[Close]

BLAST of CSPI04G22410 vs. NCBI nr
Match: XP_038898315.1 (peptide methionine sulfoxide reductase B1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 367.9 bits (943), Expect = 5.9e-98
Identity = 183/210 (87.14%), Postives = 192/210 (91.43%), Query Frame = 0


Query: 1   MSTMAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSS 60
           MS MAARSLN S LPTGFIS+SKTQQ+F F+S SSPPSF + KNKKLSSSS RVMG SSS
Sbjct: 1   MSAMAARSLNSSILPTGFISSSKTQQNFKFLSLSSPPSFGSPKNKKLSSSSVRVMG-SSS 60

Query: 61  SSQKPDGIK--------EAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNT 120
           SSQ+PDGI+        EAGTV+YKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNT
Sbjct: 61  SSQRPDGIQEAQLPVGAEAGTVDYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNT 120

Query: 121 KTAGIYHCICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVC 180
           KT GIYHCICC+TPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVC
Sbjct: 121 KTPGIYHCICCNTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVC 180

Query: 181 DAHLGHVFNDGPPPTGKRYCINSAALKLKP 203
           DAHLGHVF+DGPPPTGKRYCINSAALKLKP
Sbjct: 181 DAHLGHVFDDGPPPTGKRYCINSAALKLKP 209

[Close]

BLAST of CSPI04G22410 vs. NCBI nr
Match: XP_023534949.1 (peptide methionine sulfoxide reductase B1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 347.4 bits (890), Expect = 8.2e-92
Identity = 169/199 (84.92%), Postives = 182/199 (91.46%), Query Frame = 0


Query: 4   MAARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSFRAIKNKKLSSSSTRVMGCSSSSSQ 63
           MA+RSLNLS +P+GFIS+SKTQQ+  FVS  SPP FRA KN+KLSSSS R MG SSSSSQ
Sbjct: 1   MASRSLNLS-IPSGFISSSKTQQNLKFVSLPSPPIFRAAKNEKLSSSSIRAMG-SSSSSQ 60

Query: 64  KPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICC 123
           +PDGI+EAGT +YKSLSDDEWKKRLT EQFYITRQKGTERAFTGEYWNTKT G Y CICC
Sbjct: 61  RPDGIREAGTADYKSLSDDEWKKRLTSEQFYITRQKGTERAFTGEYWNTKTPGTYQCICC 120

Query: 124 DTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDG 183
           +TPLFESSTKFDSGTGWPSYYQP+GDNVK+KLDLSIIFMPREEVLCA CDAHLGHVF+DG
Sbjct: 121 NTPLFESSTKFDSGTGWPSYYQPIGDNVKTKLDLSIIFMPREEVLCAACDAHLGHVFDDG 180

Query: 184 PPPTGKRYCINSAALKLKP 203
           PPPT KRYCINSAALKLKP
Sbjct: 181 PPPTVKRYCINSAALKLKP 197

[Close]

BLAST of CSPI04G22410 vs. TAIR 10
Match: AT1G53670.1 (methionine sulfoxide reductase B 1 )

HSP 1 Score: 263.5 bits (672), Expect = 1.5e-70
Identity = 128/197 (64.97%), Postives = 158/197 (80.20%), Query Frame = 0


Query: 6   ARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSF--RAIKNKKLSSSSTRVMGCSSSSSQ 65
           A S  L+ + + F+S    +   N+VS ++   F  R++   +  S S   MG SSSSS 
Sbjct: 2   ASSTRLTIIQSSFVS---ARTRLNYVSKTNHSGFACRSLSKPRNLSLSVYSMG-SSSSSP 61

Query: 66  KPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICC 125
           KPD ++EA    + SLS++EWKKRLT EQ+YITRQKGTERAFTGEYWN+KT G+Y+C+CC
Sbjct: 62  KPDNVQEAEKNEFASLSENEWKKRLTPEQYYITRQKGTERAFTGEYWNSKTPGVYNCVCC 121

Query: 126 DTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDG 185
           DTPLF+SSTKFDSGTGWPSYYQP+G+NVK+KLDLSIIFMPR+EV+CAVC+AHLGHVF+DG
Sbjct: 122 DTPLFDSSTKFDSGTGWPSYYQPIGNNVKTKLDLSIIFMPRQEVVCAVCNAHLGHVFDDG 181

Query: 186 PPPTGKRYCINSAALKL 201
           P PTGKRYC+NSAALKL
Sbjct: 182 PRPTGKRYCLNSAALKL 194

[Close]

BLAST of CSPI04G22410 vs. TAIR 10
Match: AT1G53670.2 (methionine sulfoxide reductase B 1 )

HSP 1 Score: 243.4 bits (620), Expect = 1.6e-64
Identity = 121/197 (61.42%), Postives = 151/197 (76.65%), Query Frame = 0


Query: 6   ARSLNLSFLPTGFISTSKTQQSFNFVSFSSPPSF--RAIKNKKLSSSSTRVMGCSSSSSQ 65
           A S  L+ + + F+S    +   N+VS ++   F  R++   +  S S   MG SSSSS 
Sbjct: 2   ASSTRLTIIQSSFVS---ARTRLNYVSKTNHSGFACRSLSKPRNLSLSVYSMG-SSSSSP 61

Query: 66  KPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICC 125
           KPD ++EA    + SLS++EWKKRLT EQ+YITRQKGTERAFTGEYWN+KT G+Y+C+CC
Sbjct: 62  KPDNVQEAEKNEFASLSENEWKKRLTPEQYYITRQKGTERAFTGEYWNSKTPGVYNCVCC 121

Query: 126 DTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVFNDG 185
           DTPLF+       GTGWPSYYQP+G+NVK+KLDLSIIFMPR+EV+CAVC+AHLGHVF+DG
Sbjct: 122 DTPLFD-------GTGWPSYYQPIGNNVKTKLDLSIIFMPRQEVVCAVCNAHLGHVFDDG 181

Query: 186 PPPTGKRYCINSAALKL 201
           P PTGKRYC+NSAALKL
Sbjct: 182 PRPTGKRYCLNSAALKL 187

[Close]

BLAST of CSPI04G22410 vs. TAIR 10
Match: AT4G04830.1 (methionine sulfoxide reductase B5 )

HSP 1 Score: 118.6 bits (296), Expect = 5.8e-27
Identity = 56/127 (44.09%), Postives = 80/127 (62.99%), Query Frame = 0


Query: 80  SDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFESSTKFDSGTG 139
           +++EW+  L+ EQF I RQKGTE+  TGEY      GI+ C+ C TPL++S+TKFDSG G
Sbjct: 11  TEEEWRAVLSPEQFRILRQKGTEKPGTGEYDKFFEEGIFDCVGCKTPLYKSTTKFDSGCG 70

Query: 140 WPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF-NDG-PPPTGKRYCINSAA 199
           WP++++ +   +    D       R E+ CA CD HLGHVF  +G   PT +R+C+NS +
Sbjct: 71  WPAFFEGLPGAINRTPDPD---GRRTEITCAACDGHLGHVFKGEGYGNPTDERHCVNSVS 130

Query: 200 LKLKPDK 205
           +   P K
Sbjct: 131 ISFNPAK 134

[Close]

BLAST of CSPI04G22410 vs. TAIR 10
Match: AT4G04800.1 (methionine sulfoxide reductase B3 )

HSP 1 Score: 115.5 bits (288), Expect = 4.9e-26
Identity = 60/148 (40.54%), Postives = 85/148 (57.43%), Query Frame = 0


Query: 57  CSSSSSQKPDGIKEAGTVNYKSLSDDEWKKRLTGEQFYITRQKGTERAFTGEYWNTKTAG 116
           C SS       +   G+V      D+EW+  L+ EQF I RQKGTE   TGEY N    G
Sbjct: 30  CLSSGVASTVAMAAPGSV---QKGDEEWRAILSPEQFRILRQKGTEYPGTGEYVNFDKEG 89

Query: 117 IYHCICCDTPLFESSTKFDSGTGWPSYYQPVGDNVKSKLDLSIIFMPREEVLCAVCDAHL 176
           +Y C+ C+ PL++S+TKF++G GWP++++ +   +    D       R E+ CA C  HL
Sbjct: 90  VYGCVGCNAPLYKSTTKFNAGCGWPAFFEGIPGAITRTTDPD---GRRIEINCATCGGHL 149

Query: 177 GHVF-NDG-PPPTGKRYCINSAALKLKP 203
           GHVF  +G   PT +R+C+NS +LK  P
Sbjct: 150 GHVFKGEGFATPTDERHCVNSVSLKFTP 171

[Close]

BLAST of CSPI04G22410 vs. TAIR 10
Match: AT4G21860.1 (methionine sulfoxide reductase B 2 )

HSP 1 Score: 113.6 bits (283), Expect = 1.9e-25
Identity = 69/178 (38.76%), Postives = 98/178 (55.06%), Query Frame = 0


Query: 29  NFVSFSSPP-SFRAIKNKKLSSSSTRVMGCSSSSSQKPDGIKEAGTVNYKSLSDDEWKKR 88
           N + FSS P SF +++         R++   SS+   P+ + +          ++EW+  
Sbjct: 39  NLLRFSSSPLSFPSLRR---GFHGGRIVAMGSSA---PESVNK---------PEEEWRAI 98

Query: 89  LTGEQFYITRQKGTERAFTGEYWNTKTAGIYHCICCDTPLFESSTKFDSGTGWPSYYQPV 148
           L+ EQF I RQKGTE   TGEY      GIY C  C TPL++S+TKFDSG GWP+++  +
Sbjct: 99  LSPEQFRILRQKGTEYPGTGEYNKVFDDGIYCCAGCGTPLYKSTTKFDSGCGWPAFFDGL 158

Query: 149 GDNVKSKLDLSIIFMPREEVLCAVCDAHLGHVF-NDG-PPPTGKRYCINSAALKLKPD 204
              +    D       R E+ CA C  HLGHVF  +G P PT +R+C+NS +LK  P+
Sbjct: 159 PGAITRTPDPD---GRRIEITCAACGGHLGHVFKGEGFPTPTDERHCVNSISLKFTPE 198

The following BLAST results are available for this feature:

BLAST of CSPI04G22410 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C8M2	2.0e-69	64.97	Peptide methionine sulfoxide reductase B1, chloroplastic OS=Arabidopsis thaliana...	[more]
Q0DC89	2.3e-68	76.54	Peptide methionine sulfoxide reductase B1, chloroplastic OS=Oryza sativa subsp. ...	[more]
Q8DJK9	1.4e-46	66.40	Peptide methionine sulfoxide reductase MsrB OS=Thermosynechococcus elongatus (st...	[more]
B0BYW4	2.5e-43	65.32	Peptide methionine sulfoxide reductase MsrB OS=Acaryochloris marina (strain MBIC...	[more]
Q78J03	1.4e-41	48.28	Methionine-R-sulfoxide reductase B2, mitochondrial OS=Mus musculus OX=10090 GN=M...	[more]

BLAST of CSPI04G22410 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L4I4	2.2e-114	100.00	Peptide-methionine (R)-S-oxide reductase OS=Cucumis sativus OX=3659 GN=Csa_4G622...	[more]
A0A1S3BUL6	3.0e-108	95.59	Peptide-methionine (R)-S-oxide reductase OS=Cucumis melo OX=3656 GN=LOC103493463...	[more]
A0A6J1FBY9	3.4e-91	84.42	Peptide-methionine (R)-S-oxide reductase OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1BQM8	1.3e-82	81.09	Peptide-methionine (R)-S-oxide reductase OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A5D3D9X6	6.4e-82	97.28	Peptide-methionine (R)-S-oxide reductase OS=Cucumis melo var. makuwa OX=1194695 ...	[more]

BLAST of CSPI04G22410 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146010.2	4.5e-114	100.00	peptide methionine sulfoxide reductase B1, chloroplastic [Cucumis sativus] >KGN5...	[more]
XP_008452427.1	6.3e-108	95.59	PREDICTED: peptide methionine sulfoxide reductase B1, chloroplastic [Cucumis mel...	[more]
XP_038898316.1	3.7e-100	90.59	peptide methionine sulfoxide reductase B1, chloroplastic isoform X2 [Benincasa h...	[more]
XP_038898315.1	5.9e-98	87.14	peptide methionine sulfoxide reductase B1, chloroplastic isoform X1 [Benincasa h...	[more]
XP_023534949.1	8.2e-92	84.92	peptide methionine sulfoxide reductase B1, chloroplastic [Cucurbita pepo subsp. ...	[more]

BLAST of CSPI04G22410 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G53670.1	1.5e-70	64.97	methionine sulfoxide reductase B 1	[more]
AT1G53670.2	1.6e-64	61.42	methionine sulfoxide reductase B 1	[more]
AT4G04830.1	5.8e-27	44.09	methionine sulfoxide reductase B5	[more]
AT4G04800.1	4.9e-26	40.54	methionine sulfoxide reductase B3	[more]
AT4G21860.1	1.9e-25	38.76	methionine sulfoxide reductase B 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 144..144
None	No IPR available	GENE3D	2.170.150.20	Peptide methionine sulfoxide reductase.	coord: 11..144 e-value: 1.9E-59 score: 201.5
None	No IPR available	PANTHER	PTHR10173:SF52	METHIONINE-R-SULFOXIDE REDUCTASE B1	coord: 13..143
IPR002579	Peptide methionine sulphoxide reductase MrsB	PFAM	PF01641	SelR	coord: 23..142 e-value: 3.6E-52 score: 175.5
IPR002579	Peptide methionine sulphoxide reductase MrsB	TIGRFAM	TIGR00357	TIGR00357	coord: 19..142 e-value: 9.4E-56 score: 185.5
IPR002579	Peptide methionine sulphoxide reductase MrsB	PROSITE	PS51790	MSRB	coord: 21..143 score: 73.200645
IPR028427	Peptide methionine sulfoxide reductase	PANTHER	PTHR10173	METHIONINE SULFOXIDE REDUCTASE	coord: 13..143
IPR011057	Mss4-like superfamily	SUPERFAMILY	51316	Mss4-like	coord: 19..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G22410.1	CSPI04G22410.1	mRNA
CSPI04G22410.2	CSPI04G22410.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030091	protein repair
biological_process	GO:0006979	response to oxidative stress
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0033743	peptide-methionine (R)-S-oxide reductase activity
molecular_function	GO:0016671	oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor