CSPI03G30280.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G30280.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Chaperone protein dnaJ 8
Location	Chr3: 27678224 .. 27680167 (+)
Sequence length	1212
RNA-Seq Expression	CSPI03G30280.1
Synteny	CSPI03G30280.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAATAATAGAAAAGAAGATAAGAAGAGAATAAAAGTCTGATGGAAGGAACACGTCATCTCTCTCTCTCTCTCTAAAATTGACCCATTGGATAAGAACCGACGATCTCGCCCCTCCACACCCTCCCTATTCCCAATCTTCTATTTATTTCCCTTCCCCTTCCCTTTCCTTTTCCTCCATTCTTCATTCCAACGTTACTACACTTTCCTCTTCCTTTCAACACCCTCCCATTTCCAAAATTAATGGCCTCCGTCGCTACTGCTGGATTCATCGCACCATCTTCCTCTTCCTCTTCATGTTTTTCCCATATTAATCGTCCAAACAAGAATCCGACTTCCACTAATCAATTTAGAGTTTCTTGTTCTTATTCTTCTTCTTCCGTCGCCGATCCTTATCGAACTCTTCGGATTCAACCCGGTTCCTCTGAATCTGAAGTCAAAAAAGCCTTCCGACGTCTCGCTCTTAAGGTTTCTTCTTTTCTTTCTTTCCATTTCTTTATATTACAGGATTTGTTCCATCCTACTGATTCTTACGATTTCTTTGATTCTTTTTTTTATCAGTATCATCCTGATGTTTGTAAAGGGAGAAATTGTGGCGTTCAGTTTGAACAAATCAACGAAGCTTACGTTGTACGTGATCATACCCTCTTTTTTCTTTTTAATTATTTACTCAAATTTGAATCAATTATTGAAATGATTGATTTTTTTTTTCTTTCTTTCTTATAATAATAATAATTGTGCACCTATTATTGCAATAACTAATTTTTGTTTCTTTCCTCACATAATTACTAAACTAATTAAATATATAGACATTTTAGAGGAAATAGTTTGAATTTCACTATATGTATCGGAATTGAGTGTTTGATTAGGTTCATGTCGGTTTCATAGTGTTTTCGGTCATTCTATCATTGATTTTCCTATACCTATTTGTCGTATATCATATCAGGTTAAATTATATACATACACACACTTGCACTTTGTCTTTTGCTTTTATTGGGTCCCAGATTGACCCAAATTTGTTTAAGCCTTTTTATGTAAATTGCAAATTTGATCGTAGCGCTGTGTTGGTTATCATAGATTGGGTAGAGATTAAGTTGGGTCCCAAACACTTCCACCGTGTTTGTCTTATACATAATATCTACTATTGGCCTATTTATAAATTCTAATAATCGATCAAATTTGTATGTGAATTGAATTCTTGCAACAGATCTGTGTTATGAAGATAGTTTGAATTTTGCTAACGAGGTGATTTTTGATTTGGTGAAACAGATTGTGATGAATAACTTAAGAGGTATAGCAACACCAATAGAAGAGACATATGAAACAAAATATTATGAAGGGACTGATGAACCGACGAGAAAATATGGGGAACCAGATTGGGACTCGTATGAAGAATGGATGGGATATGAAGGAGCGTGGATGGGTGATTATTCATCTCAATATTAATCCTGAAATTTAAAACCCAAAAACAAAAAATTAAAAGTCAGTTATGATTTGCACATAGAATTGGTCTTTAATCTATCAACATCATCAACAACCAACGGTTGTGATGATTTGGTTTGGCTTTGTATATATGGTGGAAAAAGAGGGGAAGTTGAAAATTGGTATTAATTCACTAATTTGTGTACCCCTTTATTTTAGTGGTGGTGGTGTCGTTGTTGTACATATGGTCAAGAACAGAGCCTTTTGGTCTTTTAATAATATGAAAAGGCTTTGAAGGAATGGCCTATCAAAGTTGTGGTGGGGCATTTGTGTTTTTATCTTTTTTTAAACTCTTGTCTGGTTGTTTGCCCAATAGAGGAGGTGGTGCTTTTTTGTGTACTCGTTTCTGTCCAATATTATCCGTTTGTGAATAAAATTAAAAAGGTGGGGGGTAGGAATATAACTATTACCTCTGTTGAGTTTCATTTGAATGGAAAGGAATCCCCTTCGGGAATATTCTGGAA

mRNA sequence

TAAATAATAGAAAAGAAGATAAGAAGAGAATAAAAGTCTGATGGAAGGAACACGTCATCTCTCTCTCTCTCTCTAAAATTGACCCATTGGATAAGAACCGACGATCTCGCCCCTCCACACCCTCCCTATTCCCAATCTTCTATTTATTTCCCTTCCCCTTCCCTTTCCTTTTCCTCCATTCTTCATTCCAACGTTACTACACTTTCCTCTTCCTTTCAACACCCTCCCATTTCCAAAATTAATGGCCTCCGTCGCTACTGCTGGATTCATCGCACCATCTTCCTCTTCCTCTTCATGTTTTTCCCATATTAATCGTCCAAACAAGAATCCGACTTCCACTAATCAATTTAGAGTTTCTTGTTCTTATTCTTCTTCTTCCGTCGCCGATCCTTATCGAACTCTTCGGATTCAACCCGGTTCCTCTGAATCTGAAGTCAAAAAAGCCTTCCGACGTCTCGCTCTTAAGTATCATCCTGATGTTTGTAAAGGGAGAAATTGTGGCGTTCAGTTTGAACAAATCAACGAAGCTTACGTTATTGTGATGAATAACTTAAGAGGTATAGCAACACCAATAGAAGAGACATATGAAACAAAATATTATGAAGGGACTGATGAACCGACGAGAAAATATGGGGAACCAGATTGGGACTCGTATGAAGAATGGATGGGATATGAAGGAGCGTGGATGGGTGATTATTCATCTCAATATTAATCCTGAAATTTAAAACCCAAAAACAAAAAATTAAAAGTCAGTTATGATTTGCACATAGAATTGGTCTTTAATCTATCAACATCATCAACAACCAACGGTTGTGATGATTTGGTTTGGCTTTGTATATATGGTGGAAAAAGAGGGGAAGTTGAAAATTGGTATTAATTCACTAATTTGTGTACCCCTTTATTTTAGTGGTGGTGGTGTCGTTGTTGTACATATGGTCAAGAACAGAGCCTTTTGGTCTTTTAATAATATGAAAAGGCTTTGAAGGAATGGCCTATCAAAGTTGTGGTGGGGCATTTGTGTTTTTATCTTTTTTTAAACTCTTGTCTGGTTGTTTGCCCAATAGAGGAGGTGGTGCTTTTTTGTGTACTCGTTTCTGTCCAATATTATCCGTTTGTGAATAAAATTAAAAAGGTGGGGGGTAGGAATATAACTATTACCTCTGTTGAGTTTCATTTGAATGGAAAGGAATCCCCTTCGGGAATATTCTGGAA

Coding sequence (CDS)

ATGGCCTCCGTCGCTACTGCTGGATTCATCGCACCATCTTCCTCTTCCTCTTCATGTTTTTCCCATATTAATCGTCCAAACAAGAATCCGACTTCCACTAATCAATTTAGAGTTTCTTGTTCTTATTCTTCTTCTTCCGTCGCCGATCCTTATCGAACTCTTCGGATTCAACCCGGTTCCTCTGAATCTGAAGTCAAAAAAGCCTTCCGACGTCTCGCTCTTAAGTATCATCCTGATGTTTGTAAAGGGAGAAATTGTGGCGTTCAGTTTGAACAAATCAACGAAGCTTACGTTATTGTGATGAATAACTTAAGAGGTATAGCAACACCAATAGAAGAGACATATGAAACAAAATATTATGAAGGGACTGATGAACCGACGAGAAAATATGGGGAACCAGATTGGGACTCGTATGAAGAATGGATGGGATATGAAGGAGCGTGGATGGGTGATTATTCATCTCAATATTAA

Protein sequence

MASVATAGFIAPSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYYEGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY*

Homology

[Close]

BLAST of CSPI03G30280.1 vs. ExPASy Swiss-Prot
Match: Q9SAG8 (Chaperone protein dnaJ 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ8 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 7.6e-32
Identity = 80/141 (56.74%), Postives = 97/141 (68.79%), Query Frame = 0


Query: 14  SSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQPGSSESEVKKAFRRLA 73
           SSSSS F   N   KN    N+ +V CS SSSSV DPY+TL+I+P SSE EVKKAFR+LA
Sbjct: 21  SSSSSSFRLKNSRRKNTKMLNRSKVVCS-SSSSVMDPYKTLKIRPDSSEYEVKKAFRQLA 80

Query: 74  LKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYYEGTDEPTRKYGEP 133
            KYHPDVC+G NCGVQF+ INEAY IV   L+ I   +E T E + ++  DE      +P
Sbjct: 81  KKYHPDVCRGSNCGVQFQTINEAYDIV---LKQIKNQMEGTEEFEPFDVYDEGLNGMNDP 140

Query: 134 DWDSYEEWMGYEGAWMGDYSS 155
           D D++EEWMG+EGA   DYSS
Sbjct: 141 DCDTWEEWMGWEGAGTRDYSS 157

[Close]

BLAST of CSPI03G30280.1 vs. ExPASy Swiss-Prot
Match: O66921 (Chaperone protein DnaJ 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=dnaJ2 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 4.1e-09
Identity = 27/58 (46.55%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 43  SSSSVADPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIV 101
           +SS+  D Y  L +   +S+ E+KKA+RRL  KYHPD+CK   C  +F++INEAY ++
Sbjct: 2   ASSTKKDYYEILGVPRNASQEEIKKAYRRLVRKYHPDICKKPECEEKFKEINEAYQVL 59

[Close]

BLAST of CSPI03G30280.1 vs. ExPASy Swiss-Prot
Match: A5W9N6 (Curved DNA-binding protein OS=Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1) OX=351746 GN=cbpA PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 5.9e-08
Identity = 38/107 (35.51%), Postives = 57/107 (53.27%), Query Frame = 0


Query: 49  DPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIA 108
           D Y+ L ++P + E  +K A+R+LA KYHPDV K R+   +F++ NEAY ++     G A
Sbjct: 5   DYYKILGVEPTADEKAIKAAYRKLARKYHPDVSKERDAEEKFKEANEAYEVL-----GDA 64

Query: 109 TPIEETYETKYYEGTDEPTRKY-GEPDWDSYEEWMGYEGAWMGDYSS 155
               E  E + Y G  +  R +   P W+S     G+EG    D+ S
Sbjct: 65  QKRAEFDEIRKYGG--QHGRPFQAPPGWESRGGGGGFEGGDFSDFFS 104

[Close]

BLAST of CSPI03G30280.1 vs. ExPASy Swiss-Prot
Match: Q88DH7 (Curved DNA-binding protein OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) OX=160488 GN=cbpA PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 5.9e-08
Identity = 38/107 (35.51%), Postives = 57/107 (53.27%), Query Frame = 0


Query: 49  DPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIA 108
           D Y+ L ++P + E  +K A+R+LA KYHPDV K R+   +F++ NEAY ++     G A
Sbjct: 5   DYYKILGVEPTADEKAIKAAYRKLARKYHPDVSKERDAEEKFKEANEAYEVL-----GDA 64

Query: 109 TPIEETYETKYYEGTDEPTRKY-GEPDWDSYEEWMGYEGAWMGDYSS 155
               E  E + Y G  +  R +   P W+S     G+EG    D+ S
Sbjct: 65  QKRAEFDEIRKYGG--QHGRPFQAPPGWESRGGGGGFEGGDFSDFFS 104

[Close]

BLAST of CSPI03G30280.1 vs. ExPASy Swiss-Prot
Match: Q65U54 (Chaperone protein DnaJ OS=Mannheimia succiniciproducens (strain MBEL55E) OX=221988 GN=dnaJ PE=3 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 7.7e-08
Identity = 26/55 (47.27%), Postives = 39/55 (70.91%), Query Frame = 0


Query: 49  DPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQ-FEQINEAYVIVMN 103
           D Y TL +Q G+ E E+K+A++RLA+KYHPD   G     + F+++NEAY I+M+
Sbjct: 5   DYYETLGVQKGADEKEIKRAYKRLAMKYHPDRTNGDKAAEEKFKEVNEAYEILMD 59

[Close]

BLAST of CSPI03G30280.1 vs. ExPASy TrEMBL
Match: A0A0A0LD63 (Chaperone protein dnaJ 8 OS=Cucumis sativus OX=3659 GN=Csa_3G710790 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 7.5e-83
Identity = 154/156 (98.72%), Postives = 155/156 (99.36%), Query Frame = 0


Query: 1   MASVATAGFIAPSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQPGS 60
           MASVATAGFIAPSSSSSSCFSH NRPNKNPTSTNQFRVSCSYSSSS+ADPYRTLRIQPGS
Sbjct: 1   MASVATAGFIAPSSSSSSCFSHTNRPNKNPTSTNQFRVSCSYSSSSLADPYRTLRIQPGS 60

Query: 61  SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY 120
           SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY
Sbjct: 61  SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY 120

Query: 121 EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY 157
           EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY
Sbjct: 121 EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY 156

[Close]

BLAST of CSPI03G30280.1 vs. ExPASy TrEMBL
Match: A0A0A0LF08 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G710780 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.3e-71
Identity = 145/158 (91.77%), Postives = 146/158 (92.41%), Query Frame = 0


Query: 1   MASVATAGFIAP--SSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQP 60
           MASVATAGFIAP  SSSSSSCF H NRPNKN TSTNQFRVS S+ SSSVADPYRTLRIQP
Sbjct: 1   MASVATAGFIAPSSSSSSSSCFPHTNRPNKNRTSTNQFRVSSSF-SSSVADPYRTLRIQP 60

Query: 61  GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETK 120
           GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIAT I ETYETK
Sbjct: 61  GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATSI-ETYETK 120

Query: 121 YYEGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY 157
           Y EGTDEP  KYGEPDWDSYEEWMGYEGAWMGDYSSQY
Sbjct: 121 YSEGTDEPKTKYGEPDWDSYEEWMGYEGAWMGDYSSQY 156

[Close]

BLAST of CSPI03G30280.1 vs. ExPASy TrEMBL
Match: A0A5D3DUL4 (Chaperone protein dnaJ 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold901G00100 PE=4 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 1.0e-55
Identity = 122/157 (77.71%), Postives = 132/157 (84.08%), Query Frame = 0


Query: 1   MASVATAGFIA---PSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQ 60
           MASVATAGFIA    SSSSSSC  + NRP KN TS+NQ RVSCS SSSSVADPYRTLRIQ
Sbjct: 1   MASVATAGFIARSSSSSSSSSCIRNTNRPKKNRTSSNQLRVSCS-SSSSVADPYRTLRIQ 60

Query: 61  PGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYET 120
           PG+SESEVKKAFR LAL+YHPDVC+G NCGVQF QINEAY IVMNNLRGI+TPI ETYET
Sbjct: 61  PGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPI-ETYET 120

Query: 121 KYYEGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSS 155
            Y EG DEP R  G+PDWD +EEWMG+EGA + DYSS
Sbjct: 121 IYDEGIDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSS 155

[Close]

BLAST of CSPI03G30280.1 vs. ExPASy TrEMBL
Match: A0A1S3CPN7 (chaperone protein dnaJ 8, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502812 PE=4 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 1.0e-55
Identity = 122/157 (77.71%), Postives = 132/157 (84.08%), Query Frame = 0


Query: 1   MASVATAGFIA---PSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQ 60
           MASVATAGFIA    SSSSSSC  + NRP KN TS+NQ RVSCS SSSSVADPYRTLRIQ
Sbjct: 1   MASVATAGFIARSSSSSSSSSCIRNTNRPKKNRTSSNQLRVSCS-SSSSVADPYRTLRIQ 60

Query: 61  PGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYET 120
           PG+SESEVKKAFR LAL+YHPDVC+G NCGVQF QINEAY IVMNNLRGI+TPI ETYET
Sbjct: 61  PGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPI-ETYET 120

Query: 121 KYYEGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSS 155
            Y EG DEP R  G+PDWD +EEWMG+EGA + DYSS
Sbjct: 121 IYDEGIDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSS 155

[Close]

BLAST of CSPI03G30280.1 vs. ExPASy TrEMBL
Match: A0A6J1GC69 (chaperone protein dnaJ 8, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452806 PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.9e-46
Identity = 111/156 (71.15%), Postives = 126/156 (80.77%), Query Frame = 0


Query: 1   MASVATAGFIAPSSSSSSCFSHINRPNK-NPTSTNQFRVS-CSYSSSSVADPYRTLRIQP 60
           MASV+TAGFIA  + SSS     NR NK + +S NQ R S  SYSSSS+ADPYRTLRIQP
Sbjct: 1   MASVSTAGFIA-RTGSSSWIRQRNRANKLHRSSRNQVRASYSSYSSSSMADPYRTLRIQP 60

Query: 61  GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETK 120
           G+SESEVKKAFR+LAL+YHPDVCKG NCGVQF QINEAY IVMNNLRGI+ PI ETY+T 
Sbjct: 61  GASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMPI-ETYDT- 120

Query: 121 YYEGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSS 155
           Y +G DEP R  G+PDWD +EEWMG+EGA + DYSS
Sbjct: 121 YDDGGDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSS 153

[Close]

BLAST of CSPI03G30280.1 vs. NCBI nr
Match: XP_004137653.2 (chaperone protein dnaJ 8, chloroplastic [Cucumis sativus] >KGN58652.1 hypothetical protein Csa_001598 [Cucumis sativus])

HSP 1 Score: 316.2 bits (809), Expect = 1.6e-82
Identity = 154/156 (98.72%), Postives = 155/156 (99.36%), Query Frame = 0


Query: 1   MASVATAGFIAPSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQPGS 60
           MASVATAGFIAPSSSSSSCFSH NRPNKNPTSTNQFRVSCSYSSSS+ADPYRTLRIQPGS
Sbjct: 1   MASVATAGFIAPSSSSSSCFSHTNRPNKNPTSTNQFRVSCSYSSSSLADPYRTLRIQPGS 60

Query: 61  SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY 120
           SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY
Sbjct: 61  SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY 120

Query: 121 EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY 157
           EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY
Sbjct: 121 EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY 156

[Close]

BLAST of CSPI03G30280.1 vs. NCBI nr
Match: XP_011651813.1 (chaperone protein dnaJ 8, chloroplastic [Cucumis sativus] >KGN58651.1 hypothetical protein Csa_002647 [Cucumis sativus])

HSP 1 Score: 278.1 bits (710), Expect = 4.7e-71
Identity = 145/158 (91.77%), Postives = 146/158 (92.41%), Query Frame = 0


Query: 1   MASVATAGFIAP--SSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQP 60
           MASVATAGFIAP  SSSSSSCF H NRPNKN TSTNQFRVS S+ SSSVADPYRTLRIQP
Sbjct: 1   MASVATAGFIAPSSSSSSSSCFPHTNRPNKNRTSTNQFRVSSSF-SSSVADPYRTLRIQP 60

Query: 61  GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETK 120
           GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIAT I ETYETK
Sbjct: 61  GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATSI-ETYETK 120

Query: 121 YYEGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY 157
           Y EGTDEP  KYGEPDWDSYEEWMGYEGAWMGDYSSQY
Sbjct: 121 YSEGTDEPKTKYGEPDWDSYEEWMGYEGAWMGDYSSQY 156

[Close]

BLAST of CSPI03G30280.1 vs. NCBI nr
Match: XP_008465135.1 (PREDICTED: chaperone protein dnaJ 8, chloroplastic [Cucumis melo] >TYK27005.1 chaperone protein dnaJ 8 [Cucumis melo var. makuwa])

HSP 1 Score: 226.1 bits (575), Expect = 2.1e-55
Identity = 122/157 (77.71%), Postives = 132/157 (84.08%), Query Frame = 0


Query: 1   MASVATAGFIA---PSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQ 60
           MASVATAGFIA    SSSSSSC  + NRP KN TS+NQ RVSCS SSSSVADPYRTLRIQ
Sbjct: 1   MASVATAGFIARSSSSSSSSSCIRNTNRPKKNRTSSNQLRVSCS-SSSSVADPYRTLRIQ 60

Query: 61  PGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYET 120
           PG+SESEVKKAFR LAL+YHPDVC+G NCGVQF QINEAY IVMNNLRGI+TPI ETYET
Sbjct: 61  PGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPI-ETYET 120

Query: 121 KYYEGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSS 155
            Y EG DEP R  G+PDWD +EEWMG+EGA + DYSS
Sbjct: 121 IYDEGIDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSS 155

[Close]

BLAST of CSPI03G30280.1 vs. NCBI nr
Match: XP_038906426.1 (chaperone protein dnaJ 8, chloroplastic [Benincasa hispida])

HSP 1 Score: 213.0 bits (541), Expect = 1.9e-51
Identity = 119/159 (74.84%), Postives = 129/159 (81.13%), Query Frame = 0


Query: 1   MASVATAGFIA--PSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSS---SSVADPYRTLR 60
           MASVATAGFIA   SSSSSS   H NRP K+ +STNQ RVSCS SS   SSV DPYRTLR
Sbjct: 1   MASVATAGFIARSGSSSSSSWIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYRTLR 60

Query: 61  IQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETY 120
           IQPG+SESEVKKAFR LAL+YHPDVC+G NCGVQF QINEAY IVMNNLRGI+TPI ETY
Sbjct: 61  IQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPI-ETY 120

Query: 121 ETKYYEGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSS 155
           ET Y EG DEP R   +PDWD +EEWMG+EGA + DYSS
Sbjct: 121 ET-YDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSS 157

[Close]

BLAST of CSPI03G30280.1 vs. NCBI nr
Match: XP_023525914.1 (chaperone protein dnaJ 8, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG6606847.1 Chaperone protein dnaJ 8, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036551.1 Chaperone protein dnaJ 8, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 196.4 bits (498), Expect = 1.8e-46
Identity = 112/156 (71.79%), Postives = 126/156 (80.77%), Query Frame = 0


Query: 1   MASVATAGFIAPSSSSSSCFSHINRPNK-NPTSTNQFRVS-CSYSSSSVADPYRTLRIQP 60
           MASV+TAGFIA  + SSS     NR NK + +S NQ R S  SYSSSS+ADPYRTLRIQP
Sbjct: 1   MASVSTAGFIA-RTGSSSWIRQRNRANKLHRSSRNQVRASYSSYSSSSMADPYRTLRIQP 60

Query: 61  GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETK 120
           G+SESEVKKAFR+LAL+YHPDVCKG NCGVQF QINEAY IVMNNLRGI+ PI ETYET 
Sbjct: 61  GASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMPI-ETYET- 120

Query: 121 YYEGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSS 155
           Y +G DEP R  G+PDWD +EEWMG+EGA + DYSS
Sbjct: 121 YDDGGDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSS 153

[Close]

BLAST of CSPI03G30280.1 vs. TAIR 10
Match: AT1G80920.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 5.4e-33
Identity = 80/141 (56.74%), Postives = 97/141 (68.79%), Query Frame = 0


Query: 14  SSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQPGSSESEVKKAFRRLA 73
           SSSSS F   N   KN    N+ +V CS SSSSV DPY+TL+I+P SSE EVKKAFR+LA
Sbjct: 21  SSSSSSFRLKNSRRKNTKMLNRSKVVCS-SSSSVMDPYKTLKIRPDSSEYEVKKAFRQLA 80

Query: 74  LKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYYEGTDEPTRKYGEP 133
            KYHPDVC+G NCGVQF+ INEAY IV   L+ I   +E T E + ++  DE      +P
Sbjct: 81  KKYHPDVCRGSNCGVQFQTINEAYDIV---LKQIKNQMEGTEEFEPFDVYDEGLNGMNDP 140

Query: 134 DWDSYEEWMGYEGAWMGDYSS 155
           D D++EEWMG+EGA   DYSS
Sbjct: 141 DCDTWEEWMGWEGAGTRDYSS 157

[Close]

BLAST of CSPI03G30280.1 vs. TAIR 10
Match: AT1G72070.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.1e-09
Identity = 42/112 (37.50%), Postives = 58/112 (51.79%), Query Frame = 0


Query: 35  QFRVSCSYSSSSVADPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQIN 94
           QF   C  S       Y  L + P +S++EVK+AF+RLALKYHPDV KG++    F++I 
Sbjct: 27  QFPTRCCLSPD--LSHYTVLGLTPLASQTEVKRAFKRLALKYHPDVHKGQD--KDFKEIK 86

Query: 95  EAYVIVMNNLRGIATPIEETYETKYYEGTDEPTRKYGEPDWDSYEEWMGYEG 147
            AY  +M   +     +E T              + GE   D +EEWMG+EG
Sbjct: 87  SAYECLMQKFKKEEEEMEIT--------------EMGE--IDEWEEWMGFEG 118

[Close]

BLAST of CSPI03G30280.1 vs. TAIR 10
Match: AT5G59610.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 1.0e-07
Identity = 26/72 (36.11%), Postives = 45/72 (62.50%), Query Frame = 0


Query: 32  STNQFRVSCSYSSSSVADPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFE 91
           S+ Q R +  + + + + PY  L + P ++  ++K+A+R+LALKYHPDV K  N   +F 
Sbjct: 56  SSIQRRRNRIFVTRARSSPYEILGVSPSATPQDIKRAYRKLALKYHPDVNKEANAQEKFL 115

Query: 92  QINEAYVIVMNN 104
           +I  AY  ++N+
Sbjct: 116 KIKHAYTTLINS 127

[Close]

BLAST of CSPI03G30280.1 vs. TAIR 10
Match: AT5G59610.2 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 1.0e-07
Identity = 26/72 (36.11%), Postives = 45/72 (62.50%), Query Frame = 0


Query: 32  STNQFRVSCSYSSSSVADPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFE 91
           S+ Q R +  + + + + PY  L + P ++  ++K+A+R+LALKYHPDV K  N   +F 
Sbjct: 56  SSIQRRRNRIFVTRARSSPYEILGVSPSATPQDIKRAYRKLALKYHPDVNKEANAQEKFL 115

Query: 92  QINEAYVIVMNN 104
           +I  AY  ++N+
Sbjct: 116 KIKHAYTTLINS 127

[Close]

BLAST of CSPI03G30280.1 vs. TAIR 10
Match: AT3G08970.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 50.4 bits (119), Expect = 1.5e-06
Identity = 21/52 (40.38%), Postives = 33/52 (63.46%), Query Frame = 0


Query: 49  DPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIV 101
           DPY+ L +   + + E++KAF + +LKYHPD  K +    +F +IN AY I+
Sbjct: 27  DPYKVLGVSKDAKQREIQKAFHKQSLKYHPDKNKDKGAQEKFAEINNAYEIL 78

The following BLAST results are available for this feature:

BLAST of CSPI03G30280.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SAG8	7.6e-32	56.74	Chaperone protein dnaJ 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ8 ...	[more]
O66921	4.1e-09	46.55	Chaperone protein DnaJ 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=dnaJ2 PE=...	[more]
A5W9N6	5.9e-08	35.51	Curved DNA-binding protein OS=Pseudomonas putida (strain ATCC 700007 / DSM 6899 ...	[more]
Q88DH7	5.9e-08	35.51	Curved DNA-binding protein OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 /...	[more]
Q65U54	7.7e-08	47.27	Chaperone protein DnaJ OS=Mannheimia succiniciproducens (strain MBEL55E) OX=2219...	[more]

BLAST of CSPI03G30280.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LD63	7.5e-83	98.72	Chaperone protein dnaJ 8 OS=Cucumis sativus OX=3659 GN=Csa_3G710790 PE=4 SV=1	[more]
A0A0A0LF08	2.3e-71	91.77	J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G710780 PE=4 SV=1	[more]
A0A5D3DUL4	1.0e-55	77.71	Chaperone protein dnaJ 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3CPN7	1.0e-55	77.71	chaperone protein dnaJ 8, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502812 ...	[more]
A0A6J1GC69	1.9e-46	71.15	chaperone protein dnaJ 8, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of CSPI03G30280.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137653.2	1.6e-82	98.72	chaperone protein dnaJ 8, chloroplastic [Cucumis sativus] >KGN58652.1 hypothetic...	[more]
XP_011651813.1	4.7e-71	91.77	chaperone protein dnaJ 8, chloroplastic [Cucumis sativus] >KGN58651.1 hypothetic...	[more]
XP_008465135.1	2.1e-55	77.71	PREDICTED: chaperone protein dnaJ 8, chloroplastic [Cucumis melo] >TYK27005.1 ch...	[more]
XP_038906426.1	1.9e-51	74.84	chaperone protein dnaJ 8, chloroplastic [Benincasa hispida]	[more]
XP_023525914.1	1.8e-46	71.79	chaperone protein dnaJ 8, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG66...	[more]

BLAST of CSPI03G30280.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80920.1	5.4e-33	56.74	Chaperone DnaJ-domain superfamily protein	[more]
AT1G72070.1	1.1e-09	37.50	Chaperone DnaJ-domain superfamily protein	[more]
AT5G59610.1	1.0e-07	36.11	Chaperone DnaJ-domain superfamily protein	[more]
AT5G59610.2	1.0e-07	36.11	Chaperone DnaJ-domain superfamily protein	[more]
AT3G08970.1	1.5e-06	40.38	DNAJ heat shock N-terminal domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 51..69 score: 34.72 coord: 85..105 score: 22.59 coord: 69..84 score: 50.08
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 48..105 e-value: 1.2E-15 score: 68.0
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 49..102 e-value: 3.3E-14 score: 52.7
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 49..119 score: 13.17625
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 49..97 e-value: 1.64985E-16 score: 67.1853
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 23..106 e-value: 7.3E-17 score: 63.1
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 40..103
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 8..22
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR24074:SF37	HEAT SHOCK PROTEIN BINDING PROTEIN	coord: 13..155
None	No IPR available	PANTHER	PTHR24074	CO-CHAPERONE PROTEIN DJLA	coord: 13..155

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G30280	CSPI03G30280	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G30280.1.utr5p1	CSPI03G30280.1.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G30280.1.cds1	CSPI03G30280.1.cds1	CDS
CSPI03G30280.1.cds2	CSPI03G30280.1.cds2	CDS
CSPI03G30280.1.cds3	CSPI03G30280.1.cds3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G30280.1.utr3p1	CSPI03G30280.1.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G30280.1	CSPI03G30280.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009416	response to light stimulus
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005634	nucleus