CSPI03G05210.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G05210.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	fatty acid desaturase 4, chloroplastic
Location	Chr3: 4228228 .. 4229169 (+)
Sequence length	942
RNA-Seq Expression	CSPI03G05210.1
Synteny	CSPI03G05210.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCATCTTAGCTCAAAACAAGTTCCTTCTGAGCTCTCGGTCCCATGTTAATCCATGCAGCACTCCGATTCCACGCAGAGTTGTCTACTGCATAGCTAATACCACCGTCAATGGTGTTGCTACCAGTCCGAAGCATGCTGATGGTCAACTAGTCATTGAGCGTGAGGTTATATCTCCTACTCCACTTACATTTGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGATCAACTTGGAGTCATCGTGCGTGGGTGGCTGGTGGGTGCACCACAGTGCTAATCTCTTTGGCAAAATCAATAGTGGGTGCAGCCAGTTCACAAATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCCGGAGTATATCATTGGGGCATCGATAACTATGGTGATGCGTCAACCCCAATCTTCGGTGCTCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCCTGGACAATCACGAAGCGACAGTTCGCCAACAATTTGTACGCTTTGGCACGAGCTGTGACTTTTGCTGTACTTCCTGTAAGCCTTGCATTTGATGATCCCATTCTACATGGTTTTGTTGGTATGTGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCACAGTAAAAAGAGTGAACTACCTCCACTCGTGGTGGCAATGCAAAATGCTGGCCTGCTCGTGTCACGAACACAGCATTCAACACATCATCGTCCACCCTATAACAACAACTATTGCATAGTAAGTGGAATATGGAATAAGCTTTTGGATGAAACAAAGCTTTTTGAGGCATTGGAGATGATCTTGTACTTCAAACTTGGGCTGAGACCAAGATCTTGGACTGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTTGAACAAACTACAGCCCATTAA

mRNA sequence

ATGTCCATCTTAGCTCAAAACAAGTTCCTTCTGAGCTCTCGGTCCCATGTTAATCCATGCAGCACTCCGATTCCACGCAGAGTTGTCTACTGCATAGCTAATACCACCGTCAATGGTGTTGCTACCAGTCCGAAGCATGCTGATGGTCAACTAGTCATTGAGCGTGAGGTTATATCTCCTACTCCACTTACATTTGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGATCAACTTGGAGTCATCGTGCGTGGGTGGCTGGTGGGTGCACCACAGTGCTAATCTCTTTGGCAAAATCAATAGTGGGTGCAGCCAGTTCACAAATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCCGGAGTATATCATTGGGGCATCGATAACTATGGTGATGCGTCAACCCCAATCTTCGGTGCTCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCCTGGACAATCACGAAGCGACAGTTCGCCAACAATTTGTACGCTTTGGCACGAGCTGTGACTTTTGCTGTACTTCCTGTAAGCCTTGCATTTGATGATCCCATTCTACATGGTTTTGTTGGTATGTGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCACAGTAAAAAGAGTGAACTACCTCCACTCGTGGTGGCAATGCAAAATGCTGGCCTGCTCGTGTCACGAACACAGCATTCAACACATCATCGTCCACCCTATAACAACAACTATTGCATAGTAAGTGGAATATGGAATAAGCTTTTGGATGAAACAAAGCTTTTTGAGGCATTGGAGATGATCTTGTACTTCAAACTTGGGCTGAGACCAAGATCTTGGACTGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTTGAACAAACTACAGCCCATTAA

Coding sequence (CDS)

ATGTCCATCTTAGCTCAAAACAAGTTCCTTCTGAGCTCTCGGTCCCATGTTAATCCATGCAGCACTCCGATTCCACGCAGAGTTGTCTACTGCATAGCTAATACCACCGTCAATGGTGTTGCTACCAGTCCGAAGCATGCTGATGGTCAACTAGTCATTGAGCGTGAGGTTATATCTCCTACTCCACTTACATTTGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGATCAACTTGGAGTCATCGTGCGTGGGTGGCTGGTGGGTGCACCACAGTGCTAATCTCTTTGGCAAAATCAATAGTGGGTGCAGCCAGTTCACAAATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCCGGAGTATATCATTGGGGCATCGATAACTATGGTGATGCGTCAACCCCAATCTTCGGTGCTCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCCTGGACAATCACGAAGCGACAGTTCGCCAACAATTTGTACGCTTTGGCACGAGCTGTGACTTTTGCTGTACTTCCTGTAAGCCTTGCATTTGATGATCCCATTCTACATGGTTTTGTTGGTATGTGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCACAGTAAAAAGAGTGAACTACCTCCACTCGTGGTGGCAATGCAAAATGCTGGCCTGCTCGTGTCACGAACACAGCATTCAACACATCATCGTCCACCCTATAACAACAACTATTGCATAGTAAGTGGAATATGGAATAAGCTTTTGGATGAAACAAAGCTTTTTGAGGCATTGGAGATGATCTTGTACTTCAAACTTGGGCTGAGACCAAGATCTTGGACTGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTTGAACAAACTACAGCCCATTAA

Protein sequence

MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETEILEQTTAH*

Homology

[Close]

BLAST of CSPI03G05210.1 vs. ExPASy Swiss-Prot
Match: Q9SZ42 (Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 PE=1 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.4e-106
Identity = 183/292 (62.67%), Postives = 221/292 (75.68%), Query Frame = 0


Query: 23  PIPRRVVYCIANTTVN--------GVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSV 82
           P P R    +   TVN          +T P+    +LV+E+ + SP PL+          
Sbjct: 39  PQPNREKLLVEQRTVNLPLSNDQSLQSTKPRPNREKLVVEQRLASP-PLS---------- 98

Query: 83  LNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILADLGSGV 142
            NDP+L+STW+HR WVA GCTT+ +SLAKS++G   S + LEPALA   GYILADLGSGV
Sbjct: 99  -NDPTLKSTWTHRLWVAAGCTTLFVSLAKSVIGGFDSHLCLEPALAGYAGYILADLGSGV 158

Query: 143 YHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAF 202
           YHW IDNYGD STP+ G QIEAFQGHHKWPWTIT+RQFANNL+ALA+ +TF VLP+ LAF
Sbjct: 159 YHWAIDNYGDESTPVVGTQIEAFQGHHKWPWTITRRQFANNLHALAQVITFTVLPLDLAF 218

Query: 203 DDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPY 262
           +DP+ HGFV   + CI+FSQQFHAWAH  KS+LPPLVVA+Q+ GLLVSR QH+ HHR PY
Sbjct: 219 NDPVFHGFVCTFAFCILFSQQFHAWAHGTKSKLPPLVVALQDMGLLVSRRQHAEHHRAPY 278

Query: 263 NNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETEI 307
           NNNYCIVSG WN +LDE+K+FEALEM+ YF+LG+RPRSW+EPNS+W EETEI
Sbjct: 279 NNNYCIVSGAWNNVLDESKVFEALEMVFYFQLGVRPRSWSEPNSDWIEETEI 318

[Close]

BLAST of CSPI03G05210.1 vs. ExPASy Swiss-Prot
Match: O04584 (Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L1 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.5e-98
Identity = 168/284 (59.15%), Postives = 209/284 (73.59%), Query Frame = 0


Query: 22  TPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSVLNDPSLR 81
           T IPR         T +   T+P+     LV+E+ +++P PL         S  NDP+L+
Sbjct: 15  TNIPRSYGPTRVRVTCSVTTTNPQLNHENLVVEKRLVNP-PL---------SKNNDPTLQ 74

Query: 82  STWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILADLGSGVYHWGIDN 141
           STW+HR WVA G TT+  S AKSI+G   S +WL+PALA   GY+ ADLGSGVYHW IDN
Sbjct: 75  STWTHRLWVAAGSTTIFASFAKSIIGGFGSHLWLQPALACYAGYVFADLGSGVYHWAIDN 134

Query: 142 YGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAFDDPILHG 201
           YG ASTPI GAQ+EA QGHHK+PWTITKRQFANN Y +ARA+TF VLP++LA ++P+ H 
Sbjct: 135 YGGASTPIVGAQLEASQGHHKYPWTITKRQFANNSYTIARAITFIVLPLNLAINNPLFHS 194

Query: 202 FVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPYNNNYCIV 261
           FV   + CI+ SQQFHAWAH  KS+LPPLV+A+Q+ GLLVSR  H  HH+ PYN+NYC+V
Sbjct: 195 FVSTFAFCILLSQQFHAWAHGTKSKLPPLVMALQDMGLLVSRKDHPGHHQAPYNSNYCVV 254

Query: 262 SGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETE 306
           SG WNK+LDE+ LF+ALEM L+F+ G+RP SW EPNS+WTEETE
Sbjct: 255 SGAWNKVLDESNLFKALEMALFFQFGVRPNSWNEPNSDWTEETE 288

[Close]

BLAST of CSPI03G05210.1 vs. ExPASy Swiss-Prot
Match: O81006 (Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L2 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 3.0e-96
Identity = 167/291 (57.39%), Postives = 212/291 (72.85%), Query Frame = 0


Query: 11  LSSRSHVNPCSTPIPR--RVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNS 70
           L ++  +NP +  IPR  R  +    +T N   + P H + +LV+E+ +++P PL+    
Sbjct: 5   LQTKYTLNPITNNIPRSHRPSFLRVTSTTN---SQPNH-EMKLVVEQRLVNP-PLS---- 64

Query: 71  STYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILA 130
                  NDP+L+STW+HR WVA GCTTV +S +KSI+GA  S +WLEP+LA   GYILA
Sbjct: 65  -------NDPTLQSTWTHRLWVAAGCTTVFVSFSKSIIGAFGSHLWLEPSLAGFAGYILA 124

Query: 131 DLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVL 190
           DLGSGVYHW  DNYGD STP+ G  IE  Q HHK PWTITKRQFANNL+ +AR  T  VL
Sbjct: 125 DLGSGVYHWATDNYGDESTPLVGIHIEDSQDHHKCPWTITKRQFANNLHFMARGTTLIVL 184

Query: 191 PVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHST 250
           P+ LAFDD ++HGFV M + C++F Q FHAWAH  KS+LPPLVV +Q+ GLLVSR  H  
Sbjct: 185 PLDLAFDDHVVHGFVSMFAFCVLFCQLFHAWAHGTKSKLPPLVVGLQDIGLLVSRIHHMN 244

Query: 251 HHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSE 300
           HHR PYNNNYC+VSG+WNK+LDE+ +F+A+EM+LY +LG+RPRSWTEPN E
Sbjct: 245 HHRAPYNNNYCVVSGVWNKVLDESNVFKAMEMVLYIQLGVRPRSWTEPNYE 279

[Close]

BLAST of CSPI03G05210.1 vs. ExPASy Swiss-Prot
Match: Q9AE87 (Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 8.7e-19
Identity = 65/199 (32.66%), Postives = 89/199 (44.72%), Query Frame = 0


Query: 99  ISLAKSIVGAASSQIWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFG-AQIEAF 158
           ++L   + G   +  WL  + A L+GY+ AD  SG  HW  D +G    P+ G A I  F
Sbjct: 42  VALVYRLWGTPYAGTWLLLS-AVLLGYLAADFVSGFVHWMGDTWGSTEMPVLGKALIRPF 101

Query: 159 QGHHKWPWTITKRQFA-----NNLYALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMF 218
           + HH     IT+  F      N L +L  A+    LP+S          F+G     +M 
Sbjct: 102 REHHVDEKAITRHDFVETNGNNCLISLPVAIIALCLPMS-GPGWVFCASFLGAMIFWVMA 161

Query: 219 SQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDET 278
           + QFH W+H      P LV  +Q   L++    H  HH  PYN  YCI  G  NK L   
Sbjct: 162 TNQFHKWSHMDSP--PALVGFLQRVHLILPPDHHRIHHTKPYNKYYCITVGWMNKPLTMV 221

Query: 279 KLFEALEMILYFKLGLRPR 292
             F   E ++ +  GL PR
Sbjct: 222 HFFPTAERLITWATGLLPR 236

[Close]

BLAST of CSPI03G05210.1 vs. ExPASy Swiss-Prot
Match: Q5UR78 (Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L630 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.7e-11
Identity = 47/179 (26.26%), Postives = 78/179 (43.58%), Query Frame = 0


Query: 115 LEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGAQ-IEAFQGHHKWPWTITKRQFA 174
           ++  +  ++GY+L+D   G+YHW  D Y    TPI G   I   + HH  P  + +    
Sbjct: 6   IDKFIQIVLGYLLSDFIMGIYHWIKDTYFSPFTPIIGKTFIWGSRLHHVRPRYVLEFTDK 65

Query: 175 NNLYALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVA 234
           + +   A+     + P+   F    L  F+      I  +   H + H    E P     
Sbjct: 66  DLIIDSAKWTLSWIGPLFFWFG---LTPFLVTMFIMISLNDVIHKYTHEIDHERPMWATI 125

Query: 235 MQNAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRS 293
           +Q  G   S  +H  HH  P+  NYC V+   N  L++  L+  LE  + +  G++PR+
Sbjct: 126 LQRIGFFQSHDEHHLHHIAPHEINYCPVTPYVNIWLEKINLWRKLESFVEYLTGVKPRA 181

[Close]

BLAST of CSPI03G05210.1 vs. ExPASy TrEMBL
Match: A0A0A0L6B9 (TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G077710 PE=3 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.2e-180
Identity = 310/313 (99.04%), Postives = 310/313 (99.04%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALA 120
           TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS IWLEPALA
Sbjct: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGVYHWGIDNYGDASTPIFG QIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLA DDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL
Sbjct: 181 RAVTFAVLPVSLAIDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

[Close]

BLAST of CSPI03G05210.1 vs. ExPASy TrEMBL
Match: A0A5A7SXQ7 (Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold925G00380 PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 8.3e-174
Identity = 297/313 (94.89%), Postives = 305/313 (97.44%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGVATS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVATSSKHGDGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS+IWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGVYHWGIDNYG ASTPIFG QIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLAFDDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

[Close]

BLAST of CSPI03G05210.1 vs. ExPASy TrEMBL
Match: A0A1S3CJN9 (fatty acid desaturase 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501595 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 3.2e-173
Identity = 295/313 (94.25%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGV TS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS+IWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSG+YHWGIDNYG ASTPIFG QIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLAFDDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

[Close]

BLAST of CSPI03G05210.1 vs. ExPASy TrEMBL
Match: A0A6J1DD70 (fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111019014 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.6e-153
Identity = 263/316 (83.23%), Postives = 283/316 (89.56%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANT----TVNGVATSPKHADGQLVIERE 60
           MSILAQNKFLLSSR+ VNP S PIPR++VYC+ANT          T PKHADGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60

Query: 61  VISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASS IWLE
Sbjct: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120

Query: 121 PALAALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSGVYHWGIDNYGDASTPIFG QIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQN 240
           YALARAVTF VLP++LAF DPILHGFV +CSGCIMFSQQFHAWAH KKS+LPPLVVAMQ+
Sbjct: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240

Query: 241 AGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEP 300
           AGLLVSR QHS HHRPPYNNNYCIVSGIWN+LLDETK+FEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300

Query: 301 NSEWTEETEILEQTTA 313
           N+EWTEETE +EQTTA
Sbjct: 301 NTEWTEETETVEQTTA 315

[Close]

BLAST of CSPI03G05210.1 vs. ExPASy TrEMBL
Match: A0A6J1IGF1 (fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111476701 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 8.4e-150
Identity = 261/318 (82.08%), Postives = 280/318 (88.05%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVN-----GVATSPKHADGQLVIER 60
           MSIL QNKFLLSS++HV+  S  +PR+ V CIANTTVN       A  PKH DGQLVIER
Sbjct: 1   MSILVQNKFLLSSKTHVSLWSPAVPRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60

Query: 61  EVISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWL 120
           E +SPT     NSSTYYSVLNDPSL+STWSHR WV  GCT VLISLAKSI GAASS  WL
Sbjct: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASSHTWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFG QIEAFQGHHK PWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180

Query: 181 LYALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQ 240
           LYALARAVTF VLP++LA +DPILHGFV +CSGCIMFSQQFHAWAH KKS+LPPLVVAMQ
Sbjct: 181 LYALARAVTFTVLPITLAVNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 NAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTE 300
           +AGLLVSR QHS HHRPPYNNNYCIVSGIWNKLLDETK+FEALEMIL+FKLG+RPRSWTE
Sbjct: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PNSEWTEETEILEQTTAH 314
           P+SEWTEETEI+EQTTA+
Sbjct: 301 PHSEWTEETEIIEQTTAY 318

[Close]

BLAST of CSPI03G05210.1 vs. NCBI nr
Match: XP_004152938.1 (fatty acid desaturase 4, chloroplastic [Cucumis sativus] >KGN56137.1 hypothetical protein Csa_011564 [Cucumis sativus])

HSP 1 Score: 642.1 bits (1655), Expect = 2.5e-180
Identity = 310/313 (99.04%), Postives = 310/313 (99.04%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALA 120
           TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS IWLEPALA
Sbjct: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGVYHWGIDNYGDASTPIFG QIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLA DDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL
Sbjct: 181 RAVTFAVLPVSLAIDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

[Close]

BLAST of CSPI03G05210.1 vs. NCBI nr
Match: KAA0033959.1 (fatty acid desaturase 4 [Cucumis melo var. makuwa] >TYK02585.1 fatty acid desaturase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 619.4 bits (1596), Expect = 1.7e-173
Identity = 297/313 (94.89%), Postives = 305/313 (97.44%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGVATS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVATSSKHGDGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS+IWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGVYHWGIDNYG ASTPIFG QIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLAFDDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

[Close]

BLAST of CSPI03G05210.1 vs. NCBI nr
Match: XP_008463431.1 (PREDICTED: fatty acid desaturase 4, chloroplastic [Cucumis melo])

HSP 1 Score: 617.5 bits (1591), Expect = 6.5e-173
Identity = 295/313 (94.25%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGV TS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS+IWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSG+YHWGIDNYG ASTPIFG QIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLAFDDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

[Close]

BLAST of CSPI03G05210.1 vs. NCBI nr
Match: XP_038905900.1 (fatty acid desaturase 4, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 601.7 bits (1550), Expect = 3.7e-168
Identity = 288/317 (90.85%), Postives = 301/317 (94.95%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVA----TSPKHADGQLVIERE 60
           MSILAQNKFLLSSRSHVNPCSTPIPRRV YCIAN+TVN  A    T+PKHADGQL+IERE
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVFYCIANSTVNVAAGATTTNPKHADGQLLIERE 60

Query: 61  VISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLE 120
           VISPTPLT VNSSTYYSVLNDPSLRSTWSHRAWVA GCTTVL+SLAKSIVGAASS IWLE
Sbjct: 61  VISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWLE 120

Query: 121 PALAALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNL 180
           PALAALVGYILADLGSGVYHWGIDNYG+ STPIFG QIEAFQGHHKWPWTITKRQFANN+
Sbjct: 121 PALAALVGYILADLGSGVYHWGIDNYGNVSTPIFGTQIEAFQGHHKWPWTITKRQFANNM 180

Query: 181 YALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQN 240
           YALARAVTFAVLPVSLAFDDPILHGFVG+CSGCIMFSQQFHAWAH KKSELPPLVVAMQ+
Sbjct: 181 YALARAVTFAVLPVSLAFDDPILHGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQD 240

Query: 241 AGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEP 300
           AGLLVSR QHS HHRPPYNNNYCIVSGIWNK+LDETK+FEALEMIL+FKLG+RPRSWTEP
Sbjct: 241 AGLLVSRRQHSAHHRPPYNNNYCIVSGIWNKILDETKVFEALEMILFFKLGVRPRSWTEP 300

Query: 301 NSEWTEETEILEQTTAH 314
           NS WTEETEI+EQTTAH
Sbjct: 301 NSAWTEETEIIEQTTAH 317

[Close]

BLAST of CSPI03G05210.1 vs. NCBI nr
Match: XP_022150986.1 (fatty acid desaturase 4, chloroplastic [Momordica charantia])

HSP 1 Score: 550.8 bits (1418), Expect = 7.5e-153
Identity = 263/316 (83.23%), Postives = 283/316 (89.56%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANT----TVNGVATSPKHADGQLVIERE 60
           MSILAQNKFLLSSR+ VNP S PIPR++VYC+ANT          T PKHADGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60

Query: 61  VISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASS IWLE
Sbjct: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120

Query: 121 PALAALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSGVYHWGIDNYGDASTPIFG QIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQN 240
           YALARAVTF VLP++LAF DPILHGFV +CSGCIMFSQQFHAWAH KKS+LPPLVVAMQ+
Sbjct: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240

Query: 241 AGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEP 300
           AGLLVSR QHS HHRPPYNNNYCIVSGIWN+LLDETK+FEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300

Query: 301 NSEWTEETEILEQTTA 313
           N+EWTEETE +EQTTA
Sbjct: 301 NTEWTEETETVEQTTA 315

[Close]

BLAST of CSPI03G05210.1 vs. TAIR 10
Match: AT4G27030.1 (fatty acid desaturase A )

HSP 1 Score: 387.5 bits (994), Expect = 1.0e-107
Identity = 183/292 (62.67%), Postives = 221/292 (75.68%), Query Frame = 0


Query: 23  PIPRRVVYCIANTTVN--------GVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSV 82
           P P R    +   TVN          +T P+    +LV+E+ + SP PL+          
Sbjct: 39  PQPNREKLLVEQRTVNLPLSNDQSLQSTKPRPNREKLVVEQRLASP-PLS---------- 98

Query: 83  LNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILADLGSGV 142
            NDP+L+STW+HR WVA GCTT+ +SLAKS++G   S + LEPALA   GYILADLGSGV
Sbjct: 99  -NDPTLKSTWTHRLWVAAGCTTLFVSLAKSVIGGFDSHLCLEPALAGYAGYILADLGSGV 158

Query: 143 YHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAF 202
           YHW IDNYGD STP+ G QIEAFQGHHKWPWTIT+RQFANNL+ALA+ +TF VLP+ LAF
Sbjct: 159 YHWAIDNYGDESTPVVGTQIEAFQGHHKWPWTITRRQFANNLHALAQVITFTVLPLDLAF 218

Query: 203 DDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPY 262
           +DP+ HGFV   + CI+FSQQFHAWAH  KS+LPPLVVA+Q+ GLLVSR QH+ HHR PY
Sbjct: 219 NDPVFHGFVCTFAFCILFSQQFHAWAHGTKSKLPPLVVALQDMGLLVSRRQHAEHHRAPY 278

Query: 263 NNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETEI 307
           NNNYCIVSG WN +LDE+K+FEALEM+ YF+LG+RPRSW+EPNS+W EETEI
Sbjct: 279 NNNYCIVSGAWNNVLDESKVFEALEMVFYFQLGVRPRSWSEPNSDWIEETEI 318

[Close]

BLAST of CSPI03G05210.1 vs. TAIR 10
Match: AT1G62190.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 360.1 bits (923), Expect = 1.7e-99
Identity = 168/284 (59.15%), Postives = 209/284 (73.59%), Query Frame = 0


Query: 22  TPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSVLNDPSLR 81
           T IPR         T +   T+P+     LV+E+ +++P PL         S  NDP+L+
Sbjct: 15  TNIPRSYGPTRVRVTCSVTTTNPQLNHENLVVEKRLVNP-PL---------SKNNDPTLQ 74

Query: 82  STWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILADLGSGVYHWGIDN 141
           STW+HR WVA G TT+  S AKSI+G   S +WL+PALA   GY+ ADLGSGVYHW IDN
Sbjct: 75  STWTHRLWVAAGSTTIFASFAKSIIGGFGSHLWLQPALACYAGYVFADLGSGVYHWAIDN 134

Query: 142 YGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAFDDPILHG 201
           YG ASTPI GAQ+EA QGHHK+PWTITKRQFANN Y +ARA+TF VLP++LA ++P+ H 
Sbjct: 135 YGGASTPIVGAQLEASQGHHKYPWTITKRQFANNSYTIARAITFIVLPLNLAINNPLFHS 194

Query: 202 FVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPYNNNYCIV 261
           FV   + CI+ SQQFHAWAH  KS+LPPLV+A+Q+ GLLVSR  H  HH+ PYN+NYC+V
Sbjct: 195 FVSTFAFCILLSQQFHAWAHGTKSKLPPLVMALQDMGLLVSRKDHPGHHQAPYNSNYCVV 254

Query: 262 SGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETE 306
           SG WNK+LDE+ LF+ALEM L+F+ G+RP SW EPNS+WTEETE
Sbjct: 255 SGAWNKVLDESNLFKALEMALFFQFGVRPNSWNEPNSDWTEETE 288

[Close]

BLAST of CSPI03G05210.1 vs. TAIR 10
Match: AT2G22890.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 353.2 bits (905), Expect = 2.1e-97
Identity = 167/291 (57.39%), Postives = 212/291 (72.85%), Query Frame = 0


Query: 11  LSSRSHVNPCSTPIPR--RVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNS 70
           L ++  +NP +  IPR  R  +    +T N   + P H + +LV+E+ +++P PL+    
Sbjct: 5   LQTKYTLNPITNNIPRSHRPSFLRVTSTTN---SQPNH-EMKLVVEQRLVNP-PLS---- 64

Query: 71  STYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILA 130
                  NDP+L+STW+HR WVA GCTTV +S +KSI+GA  S +WLEP+LA   GYILA
Sbjct: 65  -------NDPTLQSTWTHRLWVAAGCTTVFVSFSKSIIGAFGSHLWLEPSLAGFAGYILA 124

Query: 131 DLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVL 190
           DLGSGVYHW  DNYGD STP+ G  IE  Q HHK PWTITKRQFANNL+ +AR  T  VL
Sbjct: 125 DLGSGVYHWATDNYGDESTPLVGIHIEDSQDHHKCPWTITKRQFANNLHFMARGTTLIVL 184

Query: 191 PVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHST 250
           P+ LAFDD ++HGFV M + C++F Q FHAWAH  KS+LPPLVV +Q+ GLLVSR  H  
Sbjct: 185 PLDLAFDDHVVHGFVSMFAFCVLFCQLFHAWAHGTKSKLPPLVVGLQDIGLLVSRIHHMN 244

Query: 251 HHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSE 300
           HHR PYNNNYC+VSG+WNK+LDE+ +F+A+EM+LY +LG+RPRSWTEPN E
Sbjct: 245 HHRAPYNNNYCVVSGVWNKVLDESNVFKAMEMVLYIQLGVRPRSWTEPNYE 279

The following BLAST results are available for this feature:

BLAST of CSPI03G05210.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZ42	1.4e-106	62.67	Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 P...	[more]
O04584	2.5e-98	59.15	Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
O81006	3.0e-96	57.39	Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9AE87	8.7e-19	32.66	Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1	[more]
Q5UR78	1.7e-11	26.26	Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=...	[more]

BLAST of CSPI03G05210.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6B9	1.2e-180	99.04	TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G07...	[more]
A0A5A7SXQ7	8.3e-174	94.89	Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3CJN9	3.2e-173	94.25	fatty acid desaturase 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501595 P...	[more]
A0A6J1DD70	3.6e-153	83.23	fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1IGF1	8.4e-150	82.08	fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114767...	[more]

BLAST of CSPI03G05210.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152938.1	2.5e-180	99.04	fatty acid desaturase 4, chloroplastic [Cucumis sativus] >KGN56137.1 hypothetica...	[more]
KAA0033959.1	1.7e-173	94.89	fatty acid desaturase 4 [Cucumis melo var. makuwa] >TYK02585.1 fatty acid desatu...	[more]
XP_008463431.1	6.5e-173	94.25	PREDICTED: fatty acid desaturase 4, chloroplastic [Cucumis melo]	[more]
XP_038905900.1	3.7e-168	90.85	fatty acid desaturase 4, chloroplastic-like [Benincasa hispida]	[more]
XP_022150986.1	7.5e-153	83.23	fatty acid desaturase 4, chloroplastic [Momordica charantia]	[more]

BLAST of CSPI03G05210.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27030.1	1.0e-107	62.67	fatty acid desaturase A	[more]
AT1G62190.1	1.7e-99	59.15	Kua-ubiquitin conjugating enzyme hybrid localisation domain	[more]
AT2G22890.1	2.1e-97	57.39	Kua-ubiquitin conjugating enzyme hybrid localisation domain	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019547	B domain of TMEM189, localisation domain	PFAM	PF10520	TMEM189_B_dmain	coord: 125..293 e-value: 1.5E-64 score: 217.3
None	No IPR available	PANTHER	PTHR48140	FAMILY NOT NAMED	coord: 1..309

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G05210	CSPI03G05210	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G05210.1.cds1	CSPI03G05210.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G05210.1	CSPI03G05210.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006631	fatty acid metabolic process
cellular_component	GO:0016021	integral component of membrane